Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same

A technology of mesenchymal stem cells and culture medium, applied in biochemical equipment and methods, embryonic cells, animal cells, etc., can solve the problem of long proliferation of stem cells
CN103154241AInactive Publication Date: 2013-06-12RNL BIO
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
RNL BIO
Publication Date
2013-06-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a culture medium for culturing a mesenchymal stem cell, and more specifically, to a culture medium composition for culturing a mesenchymal stem cell, comprising a basic culture medium, L-ascorbic acid 2-phosphate, fetal bovine serum, basic fibroblast growth factor (b-FGF), non-essential amino acids (NEAA), insulin, N-acetyl-L-cysteine, calcium chloride and hydrocortisone,and a method for culturing a mesenchymal stem cell by using the same. According to the present invention, it is possible to obtain the number of mesenchymal stem cells necessary for stem cell treatment within a short time and the differentiation of mesenchymal stem cells is improved, and is useful for the treatment of cells using a stem cell.
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Description

technical field

[0001] The present invention relates to a medium for culturing mesenchymal stem cells, more particularly to a medium composition for culturing mesenchymal stem cells and a method for cultivating mesenchymal stem cells using said medium composition, wherein The medium composition contains basal medium, L-ascorbic acid 2-phosphate, fetal bovine serum, basic fibroblast growth factor (b-FGF), non-essential amino acid (NEAA), insulin, N-acetyl- L-cysteine, calcium chloride and hydrocortisone. Background technique

[0002] Stem cells refer to cells that not only have self-replication ability but also have the ability to differentiate into at least two types of cells, and can be divided into totipotent stem cells, pluripotent stem cells and multipotent stem cells ).

[0003] Adult stem cells are obtained by obtaining cells from various human organs and developing the cells into stem cells, which are characterized in that they differentiate only into specific tissu...

Claims

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