36results about How to "Improve induction efficiency" patented technology

The invention provides a piezoelectric self-feedback excitation type miniature electric field sensor, an electric field detection method and a sensor, and is applied to the technical field of electric field sensors. The piezoelectric self-feedback excitation type miniature electric field sensor comprises a substrate used for supporting a vibration pickup reference structure, a piezoelectric driving structure, a mutual shielding electric field sensing structure and an elastic beam; the piezoelectric driving structure is used for enabling the piezoelectric driving structure to deform under the condition that driving voltage is applied to the piezoelectric driving structure so as to enable the elastic beam to generate vibration; the mutual shielding electric field induction structure is used for responding to the vibration of the elastic beam, and the mutual shielding electric field induction structure outputs an induction current signal in a differential form so as to suppress common-mode noise and detect the amplitude intensity of a to-be-detected electric field; and the vibration pickup reference structure is used for responding to the vibration of the elastic beam and outputting a reference current signal so as to adjust the magnitude of the driving voltage and enable the output sensitivity of the piezoelectric self-feedback excitation type miniature electric field sensor to be a preset value.

The invention discloses a method for improving plant polyploidy induction rate and obtaining polyploidy based on leaf bud primordium cell cycle synchronization. The method comprises the following steps: carrying out pre-differentiation culture on an explant induction material; performing cell cycle synchronization excitation treatment on the leaf bud primordium cells obtained by the pre-differentiation treatment; and performing chromosome doubling treatment on the explant induction material subjected to the cell cycle synchronization excitation treatment. According to the method disclosed by the invention, an EdU staining labeling method and a flow cytometry are used for accurately judging the time for carrying out cell cycle synchronization excitation treatment on leaf bud primordium cells of an induction material, and carrying out synchronization excitation and doubling induction on the induction material in time; the dosage of a cell cycle inhibitor for synchronous excitation treatment is accurate, the treatment opportunity is accurate, the treatment duration is proper, and the polyploidy inductivity of the plant is remarkably improved; the obtained polyploidy plant is good in stability, and the method is high in repeatability.

The invention relates to the field of cell biology, and particularly discloses a method for preparing mesoderm progenitor cells from human pluripotent stem cells and an induced differentiation culture medium of the mesoderm progenitor cells. The method comprises the following steps: inoculating human pluripotent stem cells in a single cell state into a culture dish covered with matrigel; and inducing the human pluripotent stem cells to be differentiated into the mesoderm progenitor cells within 1-3 days by using the mesoderm progenitor cell induced differentiation culture medium. Wherein the mesoderm progenitor cell induced differentiation culture medium is composed of a basic culture medium and an additive, and the additive contains a B-27 additive, non-essential amino acid, glutamine, ascorbic acid, polyvinyl alcohol, a GSK-3 inhibitor and a CDK8 inhibitor. According to the method, the human pluripotent stem cells are subjected to induced differentiation by using the mesoderm progenitor cell induced differentiation culture medium with specific composition, the method does not depend on cloning of the pluripotent stem cells, a large number of high-uniformity mesoderm progenitor cells can be obtained through induced differentiation within 1-3 days, the induction efficiency is high, and the stability is high.

The invention discloses a method for efficiently promoting slash pine somatic embryo maturation, and relates to the technical field of plant breeding. According to the method, the slash pine embryogenic callus is inoculated into a somatic embryo maturation culture medium containing sulfonatin and / or activated carbon, or PEG8000 and / or chitosan oligosaccharide for somatic embryo induction culture, so that early maturation of slash pine somatic embryos can be remarkably promoted. Results of the invention show that when the concentration of the sulfokine added in the culture medium is 0.5 mg / L, the total number of mature somatic embryos is as high as 109 / dish; when 0.5 mg / L of sulfonatin and 1 mg / L of activated carbon are added at the same time, the number of mature somatic embryos is up to 288 / dish; when 120g / L of PEG 8000 and 6mg / L of chitosan oligosaccharide are added at the same time, the number of mature somatic embryos is up to 123 / dish. In conclusion, the method disclosed by the invention can efficiently promote slash pine somatic embryo maturation, and is high in somatic embryo induction efficiency and strong in practicability.

The invention belongs to the technical field of tumor vaccines, and discloses a personalized tumor vaccine based on hydrogel and a preparation method and application thereof, the preparation method comprises the following steps: adding a chemotherapeutic drug into a mixed solution of polyphenol and metal ions, uniformly mixing, adjusting the pH value of the solution, and reacting for 2-8 hours to obtain a metal polyphenol nano-carrier loaded with the chemotherapeutic drug; co-culturing the obtained metal polyphenol nano-carrier loaded with the chemotherapeutic drug and tumor cells for 12-48 hours, and inducing the tumor cells to generate immunogenic cell death, wherein the concentration of the metal polyphenol nano-carrier loaded with the chemotherapeutic drug is 1-20 [mu] g / mL; the tumor cell suspension in the ICD state and the STING agonist solution are mixed with the sodium alginate solution, and the personalized tumor vaccine is obtained. The ICD is induced in vitro by the chemotherapeutic drug, so that the toxic and side effects of systemic administration are reduced, the types of antigens are enriched, and a powerful guarantee is provided for the vaccine to activate a strong in-vivo anti-tumor immune response.

The invention discloses an optimization method of a pea tissue culture regeneration system. The optimization method comprises the following steps: step 1, preparing pea aseptic seedlings; step 2, preparing an explant; step 3, inducing cluster buds; 4, carrying out bud elongation culture; step 5, rooting culture; and 6, domesticating and transplanting regenerated seedlings. By systematically optimizing the tissue culture conditions of the peas, an efficient regeneration system is established and is suitable for multiple pea varieties, and the problem of low regeneration efficiency in the prior art is solved. By means of the optimization method, researchers can obtain high-efficiency regenerated pea plants in a short time, the success rate and rooting efficiency of tissue culture regeneration are improved, meanwhile, the survival rate of regenerated seedlings is increased, and an important technical guarantee is provided for molecular breeding and gene modification of peas and other leguminous crops.

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The present invention belongs to the technical field of tumor vaccines, and discloses a personalized tumor vaccine based on hydrogel, and a preparation method and application thereof, wherein the preparation method comprises the following steps: adding a chemotherapeutic drug to a mixed solution of polyphenols and metal ions, mixing, adjusting the pH value of the solution, reacting for 2-8 hours, and obtaining a metal polyphenol nanocarrier loaded with a chemotherapeutic drug; co-culturing the obtained metal polyphenol nanocarrier loaded with a chemotherapeutic drug with tumor cells for 12-48 hours, wherein the concentration of the metal polyphenol nanocarrier loaded with a chemotherapeutic drug is 1-20 μg / mL, inducing immunogenic cell death in tumor cells; mixing a tumor cell suspension in an ICD state and a STING agonist solution with a sodium alginate solution, and obtaining a personalized tumor vaccine. The chemotherapeutic drug induces ICD in vitro, which not only reduces the toxic and side effects of systemic administration, but also enriches the types of antigens, providing a strong guarantee for the vaccine to activate a powerful anti-tumor immune response in vivo.

The invention relates to a tissue culture technology, in particular to an awnless brome callus induction culture medium and application thereof, and the awnless brome callus induction culture medium is characterized by comprising MS, sucrose, agar, 6-BA and 2, 4-D. The induction is performed by using awnless brome seeds. The awnless brome seed callus is cultivated, a new experimental material is provided for molecular biology and genetic engineering research of awnless brome, and a solid foundation is laid for molecular biology research of awnless brome. Finally, according to the culture medium, the hormone proportion and variety difference are optimized, more calluses can be induced to be generated, the overall induction efficiency is improved, the quality of the calluses can be improved, the growth stability of the seed calluses can be kept through accurately controlled environmental conditions, the difference between batches is reduced, and the yield of the seed calluses is increased. Therefore, the method is more suitable for subsequent awnless brome regeneration or genetic engineering application.

The present disclosure provides a piezoelectric self-feedback excitation type micro electric field sensor, an electric field detection method and a sensor, and is applied to the technical field of electric field sensors. The piezoelectric self-feedback excitation type micro electric field sensor comprises: a substrate for supporting a vibration pickup reference structure, a piezoelectric driving structure, a mutual shielding electric field sensing structure and an elastic beam; the piezoelectric driving structure is used for deforming the piezoelectric driving structure to make the elastic beam vibrate when a driving voltage is applied to the piezoelectric driving structure; the mutual shielding electric field sensing structure is used for outputting a differential form of a sensing current signal in response to the vibration of the elastic beam, so as to suppress common mode noise and detect the amplitude strength of the electric field to be measured; the vibration pickup reference structure is used for outputting a reference current signal in response to the vibration of the elastic beam, so as to adjust the size of the driving voltage, so that the output sensitivity of the piezoelectric self-feedback excitation type micro electric field sensor is a predetermined value.

The invention relates to the technical field of plant tissue culture, in particular to a method for accurately culturing populus hopeiensis tissues. The method comprises the following steps: picking tender populus bolleana leaves as explants; the method comprises the following steps: pretreating an explant to obtain a non-toxic explant; performing callus induction on the non-toxic explant to obtain a callus; carrying out adventitious bud induction on the calluses by using a composite culture medium containing populus lanceolata zymolyte; in the induction process of the adventitious buds, continuously shooting the adventitious buds formed by the calluses to obtain change data of the adventitious buds; according to the change data of the adventitious buds, determining the supplementing amount of the populus lanceolata zymolyte; according to the supplementing amount of the populus lanceolata zymolyte, supplementing the populus lanceolata zymolyte into the composite culture medium to obtain adventitious buds; wherein the populus henryi zymolyte has vitamin and trace element components of the populus henryi; and carrying out rooting culture on the adventitious buds to obtain populus lanceolata tissues. According to the method, the speed of forming adventitious buds by the calluses can be increased, and the propagation rate of populus hopeiensis tissues can be increased.

This invention belongs to the field of biomedicine and regenerative medicine technology. It discloses a chimeric polypeptide, related biomaterials, and their application in inducing cardiomyocytes. The chimeric polypeptide is obtained through family homologous domain substitution and is selected from the following groups: (1) a MEF2 chimeric polypeptide with MEF2C protein as the backbone, wherein the N-terminal and C-terminal transcriptional activation domains are replaced by homologous regions of MEF2B and MEF2D, respectively; (2) a GATA chimeric polypeptide with GATA4 protein as the backbone, wherein the N-terminal transcriptional activation domain is replaced by homologous regions of GATA1 and GATA6. This invention also provides the nucleic acid encoding the polypeptide, the construct, and a method for efficiently inducing fibroblast reprogramming into cardiomyocytes under in vitro conditions. When the chimeric polypeptide of this invention is used alone or in combination, the induced cells exhibit calcium ion oscillations earlier and have higher maturity.

The invention discloses a method for inducing mature embryos of Siberian apricot to breed seedlings, comprising the following steps: explant disinfection: disinfecting apricot kernels with seed shells removed and peeling off the seed coat to obtain mature embryos; sterile seedling induction: culturing mature embryos in sterile seedling induction medium to obtain sterile seedlings; sterile seedling proliferation: culturing sterile seedlings in sterile seedling proliferation medium to obtain sterile seedlings and strong seedlings; inducing callus: culturing rectangular leaves cut from sterile seedlings and strong seedlings in callus induction medium to obtain callus; differentiating cluster buds: transferring callus to cluster bud differentiation medium to obtain cluster buds; cluster bud subculture proliferation: culturing cluster buds in cluster bud subculture medium after cutting to obtain cluster bud strong seedlings; cluster bud rooting: cutting cluster buds into single buds and transferring them to rooting medium for rooting culture. The invention can solve the technical problems of long breeding cycle, low survival rate and poor breeding stability of Siberian apricot.

The invention relates to the field of skin perception, in particular to a skin perception system for a mechanical movable component. The problems that in an existing electronic skin, when a mechanical movable component covers the electronic skin to move and encounters an obstacle, the electronic skin cannot conduct sensing treatment in time, the mechanical movable component collides with the obstacle, and the electronic skin and the mechanical movable component are damaged are solved. The sensing unit, the data processing unit and the sensing control unit are arranged, so that in the moving process of the mechanical moving component covering the electronic skin, when the surrounding encounters an obstacle, the obstacle can be accurately captured, the electronic skin can sense and process the obstacle, and after sensing of the electronic skin is completed, the obstacle can be accurately captured. Therefore, the mechanical movable component is controlled and processed, and the mechanical movable component and the electronic skin are prevented from being damaged due to the fact that the mechanical movable component collides with an obstacle.

The invention belongs to the technical field of tissue culture, and relates to a pithecellobium clypearia filament callus induction method and application, and the method comprises the following steps: taking pithecellobium clypearia buds, disinfecting and sterilizing with a disinfectant, taking pithecellobium clypearia filaments as explants in a sterile environment, inoculating the explants into an induction culture medium for dark culture to form calluses, and carrying out tissue culture to obtain the pithecellobium clypearia filament callus. And then transferring the callus to a proliferation culture medium for subculture proliferation to obtain the callus with higher quality. The induction method provided by the invention can induce pithecellobium clypearia filaments to quickly form calluses, and the induction rate of the calluses is as high as 100%; the induction method of the pithecellobium clypearia filament callus is short in cultivation period, high in induction efficiency and low in pollution rate, the research path of a pithecellobium clypearia rapid propagation technology is widened when the induction method is applied to pithecellobium clypearia cultivation, it is possible that pithecellobium clypearia filaments are regenerated into plants, and high-quality plants with excellent characters can be cultivated.

The invention relates to the technical field of cell models, and discloses a method for inducing osteoclast in vitro from mouse mononuclear macrophage leukemia cells, which comprises the following steps: S10: passage of mouse mononuclear macrophage leukemia cells: inoculating the mouse mononuclear macrophage leukemia cells into a DMEM (dulbecco's modified eagle medium) high-glucose complete medium containing special-grade fetal calf serum; performing passage; s20, inducing mouse mononuclear macrophage leukemia cells: collecting 1-5 generations of mouse mononuclear macrophage leukemia cells, inoculating the mouse mononuclear macrophage leukemia cells into a DMEM high-glucose complete medium containing special-grade fetal calf serum, and adding a nuclear factor kappa B receptor activating factor ligand into the medium after 20-24 hours; s30, continuously culturing for 40-48 hours, replacing the culture medium, and adding a nuclear factor kappa B receptor activating factor ligand at the same time; and S40, continuing to culture for 40-48 hours to obtain mature osteoclasts, so that the in-vitro osteoclast induction efficiency of RAW264.7 cells can be improved.

This invention provides a circRNA-LNP delivery system for in vivo induction of iTregs, its preparation method, and its applications. The delivery system includes circRNA encoding human TGF-β1, lipid nanoparticles encapsulating the circRNA, and IL-2 modified on the surface of the lipid nanoparticles. This invention synergistically combines retinoic acid as a functional immunomodulatory small molecule with five lipids, significantly improving the uptake efficiency of Treg precursor cells and the induction efficiency of iTregs. It can also efficiently increase the proportion of functional iTregs in diseased tissues in vivo, demonstrating excellent therapeutic effects on immunomodulatory diseases such as ulcerative colitis, and providing a novel technical pathway for the radical treatment of autoimmune and inflammatory diseases.

The invention discloses a method for artificially inducing triploid percobrachium glehniae, and belongs to the technical field of triploid fish breeding. In order to solve the problems that gonad development affects the growth and meat quality of percobrachium glehniae, reduces the commodity quality of percobrachium glehniae and the like, the invention determines the optimal starting time, the optimal hydrostatic pressure and treatment time, the optimal temperature and the optimal chemical conditions of triploid percobrachium glehniae induction in combination with the triploid proportion of percobrachium glehniae, so that the percobrachium glehniae is induced into the triploid percobrachium glehniae. The method for producing the triploid percobrachium glehniae fish through combined induction of hydrostatic pressure, temperature shock and chemicals is established. The triploid percobrachium glehniae induction method established by the invention has the advantages of clear operation process, strong practicability, high efficiency and the like, and the induction efficiency of the triploid percobrachium glehniae can reach up to 85%, so that the induction method provided by the invention is suitable for artificially and efficiently producing the triploid percobrachium glehniae and has a wide application prospect. And a new technical approach is opened up for polyploidy breeding of the percobrachium glehniae.

The invention provides a non-coding RNA combination and application thereof in mouse totipotent stem cell induction, and relates to the technical field of biology. The invention provides a non-coding RNA combination for inducing totipotency of mouse embryonic stem cells. The non-coding RNA combination comprises an enhancer RNA with a sequence as shown in SEQ ID NO: 1, an enhancer RNA with a sequence as shown in SEQ ID NO: 2 and a long-chain non-coding RNA with a sequence as shown in SEQ ID NO: 3. According to the invention, the CRISPR inhibition system is used for synergistically inhibiting the expression of the specific endogenous non-coding RNA combination for the first time, and the mouse embryonic stem cells can be efficiently promoted to be converted into 2C-like cells. Exogenous transcription factor integration is not needed, so that the safety risk of a genome is avoided; precise targeting is realized through sgRNA, and the defect of non-specific off-target of chemical small molecules is overcome; a remarkable synergistic effect is generated, and the induction efficiency of 2C-like cells can be remarkably improved to about 9.09%. The invention provides a safer, more efficient and controllable new strategy for stem cell fate reprogramming and early development research.

The invention discloses a system and a method for efficiently inducing regulatory B cells and application of the system and the method. The invention provides a system for inducing regulatory B cells, the system comprises a basal culture medium and an inducer, and the system can promote B cells to express IL-10 and reduce the generation of inflammatory factors TNF-alpha at the same time. On the basis, the invention further provides a method for obtaining the regulatory B cells through induction of the system, the obtained regulatory B cells are mainly enriched in CD19 < + > CD1c < + > B cells, and compared with the CD19 < + > CD1c <-> B cells, the regulatory B cells express IL-10 with a higher level. Besides, the CD19 + CD1c + B cell can be used for inhibiting the proliferation of PBMC (peripheral blood mononuclear cells) and reducing the release of effector T cell inflammatory factors, and is used for treating graft-versus-host diseases. The invention contributes to the acquisition of regulatory B cells and the treatment of graft versus host disease.

The invention discloses an efficient cultivation method for triploid mandarin fish and belongs to the technical field of mandarin fish breeding. In order to solve the problems that the growth and meat quality of the mandarin fish can be influenced by gonad development, the commodity quality of the mandarin fish is reduced and the like, the proper starting time, the optimal hydrostatic pressure, the optimal treatment time, the optimal temperature and the optimal chemical conditions for inducing the triploid mandarin fish are determined by combining the triploid proportion of the triploid mandarin fish; the cultivation method for producing the triploid mandarin fish through combined induction of hydrostatic pressure, temperature shock and chemicals is established. The triploid mandarin fish breeding method established by the invention is clear in operation process and high in practicability, triploid mandarin fish can be efficiently induced and generated, and the induction efficiency of the triploid mandarin fish can be up to 93%. The triploid mandarin fish breeding method provided by the invention opens up a new technical approach for mandarin fish polyploidy breeding.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a culture medium for inducing mesenchymal cells to differentiate into insulin-secreting cells and application of the culture medium. By accurately regulating and controlling the oxidation-reduction state of NAD < + > / NADH in cells, the composition proportion of exogenous fatty acid, the dynamic supply concentration of glucose and the anti-oxidative stress level, the differentiation efficiency is remarkably improved, the induction period is shortened, and the method is suitable for industrial production on the premise of not depending on recombinant protein induction factors such as Activin A and Wnt3a. And a mature beta-like cell with stable glucose stimulus dependent insulin secretion (GSIS) capability is obtained. The kit is especially suitable for human adipose tissue-derived mesenchymal stem cells, and can be used as an independent kit product for preclinical research and drug screening of diabetes cell replacement therapy.

This invention provides an amorphous Pd-MoS2 thin film, its preparation method, and its application, belonging to the field of hydrogen evolution catalyst development technology. This invention prepares an amorphous Pd-MoS2 thin film by magnetron sputtering, where Pd flakes are tightly bonded to a MoS2 target. Pd doping replaces adjacent Mo sites, altering the band structure of MoS2 and introducing conductive electronic states around the Fermi level. This enhances the metallic properties of MoS2, improving conductivity and catalytic activity, thus solving the problem of insufficient performance in existing hydrogen evolution catalysts. The amorphous Pd-MoS2 thin film provided by this invention exhibits good conductivity and excellent hydrogen evolution catalytic performance when used as a hydrogen evolution catalyst.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a method for inducing mesenchymal stem cells to differentiate into insulin-secreting cells and application of the method. According to the method, a culture system is regulated and controlled by stages, the glucose concentration is respectively regulated in different induction stages, an NAD + precursor substance, a fatty acid composition and an antioxidant factor are added, and a three-dimensional microcarrier culture mode is combined, so that the cell metabolism state, the oxidation-reduction balance and the space structure are dynamically regulated and controlled. Under the condition of not depending on recombinant protein inducing factors such as Activin A and Wnt3a, the efficient directional differentiation of the mesenchymal stem cells to the insulin-secreting cells is realized. By adopting the method disclosed by the invention, the differentiation efficiency can be improved, the induction period can be shortened, the mature beta-like cells with a stable glucose stimulation dependent insulin secretion function can be obtained, and the method is suitable for induced differentiation and related research application of the human adipose-derived mesenchymal stem cells.

The invention relates to the technical field of somatic cell reprogramming and cell transplantation therapy, in particular to a method for reprogramming rat skin fibroblasts into neuronal cells, which comprises the following steps: taking in-vitro rat embryo skin fibroblasts, and reprogramming into intermediate state cells under the action of VPA, CHIR99021, Repsox, Forskolin, GO6983, Y-27632, Melatonin and P7C3-A20; and performing continuous induced maturation on the intermediate state cells by using CHIR99021, Forskolin, Dorsomorphin, P7C3-A20, NT3, N2, B27 containing vitamin A, and cAMP, so as to obtain mature neuronal cells. The neuronal cells obtained by the method provided by the invention can survive in a Parkinson rat brain microenvironment for a long time, and have a certain repairing effect on rat dyskinesia.

The present invention relates to the field of cell culture technology, and in particular to a CIK cell culture method and application thereof. The culture method comprises the following steps: (1) isolating peripheral blood mononuclear cells (PBMC); (2) preparing culture medium A; (3) preparing culture medium B; (4) resuspending and inoculating peripheral blood mononuclear cells; and (5) collecting cell culture. The culture method of the present invention can improve the yield of CIK cells, and wherein CD3 + CD56 + The proportion of double-positive cells is high, which increases cell proliferation activity and promotes the increase of CIK cell proliferation and killing activity.

The invention provides a real-time monitoring device for a reinforcing steel bar protective layer, and relates to the field of building construction, and the device comprises a sensor module which comprises an ultrasonic thickness gauge for measuring the thickness of the reinforcing steel bar protective layer and an electromagnetic inductor for measuring the performance of a reinforcing steel bar; the communication module is used for receiving the measurement information of the sensor module and converting the measurement information into a data form for transmission; the information processing module is used for receiving and processing the data information of the communication module; the power supply module is used for providing energy support for the sensor module and the communication module; the protection shell is fixed on the reinforcement cage in the reinforcement protection layer; and the sensor module, the power supply module and the communication module are all arranged in the protective shell. The method has the effect of improving the accuracy of monitoring the safety performance of the reinforcing steel bars in the reinforcing steel bar protection layer.

The invention relates to the technical field of cell culture, in particular to a culture medium for NKT cells and a use method of the culture medium. The invention provides a culture medium for NKT cells and a use method of the culture medium. The NKT cells are prepared from PBMC cells separated from peripheral blood; the separated PBMC cells are resuspended through a culture medium and then added into the culture medium to be subjected to primary culture and then subculture, and growth active components are added at set intervals in the subculture process, so that the induction efficiency of the NKT cells can be greatly improved, amplification of the NKT cells is facilitated, and the killing efficiency of the NKT cells on cancer cells can be remarkably improved.

This invention provides a method for differentiating SAN-like cells from pluripotent stem cells in vitro. The method for differentiating SAN-like cells from pluripotent stem cells in vitro includes S1, providing a population of pluripotent stem cells; S2, performing a first contact culture on the pluripotent stem cell population to obtain mesodermal cells; S3, performing a second contact culture on the mesodermal cells to obtain pSHF cells; and S4, performing a third contact culture on the pSHF cells to obtain SAN-like cells. This invention solves at least one of the following problems in the prior art: the induction efficiency of sinoatrial node (SAN)-like cells from pluripotent stem cells is generally low, usually only 30%–50%; and the differentiation cycle is long, the operation steps are cumbersome, and a variety of small molecule additives are required. In addition, the differentiation products contain a large number of NKX2.5 positive atrial or ventricular myocytes, resulting in insufficient purity, which still requires further cell separation and purification.