11results about How to "Improve induction efficiency" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The present disclosure provides a piezoelectric self-feedback excitation type micro electric field sensor, an electric field detection method and a sensor, and is applied to the technical field of electric field sensors. The piezoelectric self-feedback excitation type micro electric field sensor comprises: a substrate for supporting a vibration pickup reference structure, a piezoelectric driving structure, a mutual shielding electric field sensing structure and an elastic beam; the piezoelectric driving structure is used for deforming the piezoelectric driving structure to make the elastic beam vibrate when a driving voltage is applied to the piezoelectric driving structure; the mutual shielding electric field sensing structure is used for outputting a differential form of a sensing current signal in response to the vibration of the elastic beam, so as to suppress common mode noise and detect the amplitude strength of the electric field to be measured; the vibration pickup reference structure is used for outputting a reference current signal in response to the vibration of the elastic beam, so as to adjust the size of the driving voltage, so that the output sensitivity of the piezoelectric self-feedback excitation type micro electric field sensor is a predetermined value.

This invention belongs to the field of biomedicine and regenerative medicine technology. It discloses a chimeric polypeptide, related biomaterials, and their application in inducing cardiomyocytes. The chimeric polypeptide is obtained through family homologous domain substitution and is selected from the following groups: (1) a MEF2 chimeric polypeptide with MEF2C protein as the backbone, wherein the N-terminal and C-terminal transcriptional activation domains are replaced by homologous regions of MEF2B and MEF2D, respectively; (2) a GATA chimeric polypeptide with GATA4 protein as the backbone, wherein the N-terminal transcriptional activation domain is replaced by homologous regions of GATA1 and GATA6. This invention also provides the nucleic acid encoding the polypeptide, the construct, and a method for efficiently inducing fibroblast reprogramming into cardiomyocytes under in vitro conditions. When the chimeric polypeptide of this invention is used alone or in combination, the induced cells exhibit calcium ion oscillations earlier and have higher maturity.

This invention provides a circRNA-LNP delivery system for in vivo induction of iTregs, its preparation method, and its applications. The delivery system includes circRNA encoding human TGF-β1, lipid nanoparticles encapsulating the circRNA, and IL-2 modified on the surface of the lipid nanoparticles. This invention synergistically combines retinoic acid as a functional immunomodulatory small molecule with five lipids, significantly improving the uptake efficiency of Treg precursor cells and the induction efficiency of iTregs. It can also efficiently increase the proportion of functional iTregs in diseased tissues in vivo, demonstrating excellent therapeutic effects on immunomodulatory diseases such as ulcerative colitis, and providing a novel technical pathway for the radical treatment of autoimmune and inflammatory diseases.

The invention provides a non-coding RNA combination and application thereof in mouse totipotent stem cell induction, and relates to the technical field of biology. The invention provides a non-coding RNA combination for inducing totipotency of mouse embryonic stem cells. The non-coding RNA combination comprises an enhancer RNA with a sequence as shown in SEQ ID NO: 1, an enhancer RNA with a sequence as shown in SEQ ID NO: 2 and a long-chain non-coding RNA with a sequence as shown in SEQ ID NO: 3. According to the invention, the CRISPR inhibition system is used for synergistically inhibiting the expression of the specific endogenous non-coding RNA combination for the first time, and the mouse embryonic stem cells can be efficiently promoted to be converted into 2C-like cells. Exogenous transcription factor integration is not needed, so that the safety risk of a genome is avoided; precise targeting is realized through sgRNA, and the defect of non-specific off-target of chemical small molecules is overcome; a remarkable synergistic effect is generated, and the induction efficiency of 2C-like cells can be remarkably improved to about 9.09%. The invention provides a safer, more efficient and controllable new strategy for stem cell fate reprogramming and early development research.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a culture medium for inducing mesenchymal cells to differentiate into insulin-secreting cells and application of the culture medium. By accurately regulating and controlling the oxidation-reduction state of NAD < + > / NADH in cells, the composition proportion of exogenous fatty acid, the dynamic supply concentration of glucose and the anti-oxidative stress level, the differentiation efficiency is remarkably improved, the induction period is shortened, and the method is suitable for industrial production on the premise of not depending on recombinant protein induction factors such as Activin A and Wnt3a. And a mature beta-like cell with stable glucose stimulus dependent insulin secretion (GSIS) capability is obtained. The kit is especially suitable for human adipose tissue-derived mesenchymal stem cells, and can be used as an independent kit product for preclinical research and drug screening of diabetes cell replacement therapy.

This invention provides an amorphous Pd-MoS2 thin film, its preparation method, and its application, belonging to the field of hydrogen evolution catalyst development technology. This invention prepares an amorphous Pd-MoS2 thin film by magnetron sputtering, where Pd flakes are tightly bonded to a MoS2 target. Pd doping replaces adjacent Mo sites, altering the band structure of MoS2 and introducing conductive electronic states around the Fermi level. This enhances the metallic properties of MoS2, improving conductivity and catalytic activity, thus solving the problem of insufficient performance in existing hydrogen evolution catalysts. The amorphous Pd-MoS2 thin film provided by this invention exhibits good conductivity and excellent hydrogen evolution catalytic performance when used as a hydrogen evolution catalyst.

The invention relates to the technical field of regenerative medicine and cell therapy, in particular to a method for inducing mesenchymal stem cells to differentiate into insulin-secreting cells and application of the method. According to the method, a culture system is regulated and controlled by stages, the glucose concentration is respectively regulated in different induction stages, an NAD + precursor substance, a fatty acid composition and an antioxidant factor are added, and a three-dimensional microcarrier culture mode is combined, so that the cell metabolism state, the oxidation-reduction balance and the space structure are dynamically regulated and controlled. Under the condition of not depending on recombinant protein inducing factors such as Activin A and Wnt3a, the efficient directional differentiation of the mesenchymal stem cells to the insulin-secreting cells is realized. By adopting the method disclosed by the invention, the differentiation efficiency can be improved, the induction period can be shortened, the mature beta-like cells with a stable glucose stimulation dependent insulin secretion function can be obtained, and the method is suitable for induced differentiation and related research application of the human adipose-derived mesenchymal stem cells.

This invention provides a method for differentiating SAN-like cells from pluripotent stem cells in vitro. The method for differentiating SAN-like cells from pluripotent stem cells in vitro includes S1, providing a population of pluripotent stem cells; S2, performing a first contact culture on the pluripotent stem cell population to obtain mesodermal cells; S3, performing a second contact culture on the mesodermal cells to obtain pSHF cells; and S4, performing a third contact culture on the pSHF cells to obtain SAN-like cells. This invention solves at least one of the following problems in the prior art: the induction efficiency of sinoatrial node (SAN)-like cells from pluripotent stem cells is generally low, usually only 30%–50%; and the differentiation cycle is long, the operation steps are cumbersome, and a variety of small molecule additives are required. In addition, the differentiation products contain a large number of NKX2.5 positive atrial or ventricular myocytes, resulting in insufficient purity, which still requires further cell separation and purification.

The application relates to the technical field of rice transplanter, and discloses a rice transplanter floating boat sensing mechanism, which comprises a floating boat and a rack, the sensing assembly comprises a T-shaped cylinder arranged on the upper side of the floating boat, the inner wall of the T-shaped cylinder is slidably provided with a sensing rod, the lower side of the sensing rod is provided with a pressure sensor, the upper end of the sensing rod is provided with a conducting rod, the outer wall of the rack is fixedly provided with a support, the two side walls of the support are rotationally connected with an electric cylinder, the output end of the electric cylinder is arranged on the upper side of the conducting rod, and the mud blocking assembly comprises a mud guard plate attached to the upper side of the floating boat and arranged on the outer wall of the conducting rod. Through the ups and downs of the floating boat and the driving of the electric cylinder, the pressure between the sensing rod and the T-shaped cylinder is maintained in a preset range, the uneven terrain in the field can be adapted, the mud and sundries can be intercepted by the mud guard plate, the situation that the sensing sensitivity is reduced due to the weight increase of the floating boat is avoided, and therefore the rice transplanting operation quality and efficiency are improved.

The application discloses a preparation method and application of a non-human primate embryoid. The preparation method of the application not only significantly improves the induction efficiency of the primate embryoid, but also can reproduce the morphology and transcriptional characteristics of the natural embryoid. In addition, the application also verifies the performance of the obtained primate embryoid, which has the typical morphology and cell lineage characteristics of the post-implantation embryo after long-term in-vitro culture, indicating that the primate embryoid can simulate the implantation process and key development events after early implantation, and can be used as a reliable model for primate embryonic research.