51 results about "Glucose stimulation" patented technology

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

The invention discloses an establishment method for a type 2 diabetes mellitus animal model. The establishment method comprises the following steps: (1) preparing a lipopolysaccharide solution with aconcentration of 100ug/ml by virtue of normal saline; (2) selecting male rats which are 4-6 weeks old, feeding by virtue of a common feed, and simultaneously injecting the lipopolysaccharide solution;(3) weighing the male rats after 28 days; (4) after the weighing, fasting for 8 hours, separating serum, and measuring blood glucose and insulin; (5) evaluating the resistance of the insulin and glucose stimulated insulin secretion capacity; (6) respectively intraperitoneally injecting an STZ solution into the male rats in the 30th day and the 32th day; and (7) determining that the establishmentof the type 2 diabetes mellitus animal model is finished when the random blood sugar is more than 16.8mmol/L. According to the establishment method, lipopolysaccharide in Escherichia coli 0111:B4 subspecies is selected and is matched with the feeding of the common feed and the STZ injection so as to successfully establish the type 2 diabetes mellitus animal mode; according to the establishment method, the operation is easy, the model establishment cost is low, the model establishment rate is high, and the model establishment period is short.