153 results about "Cell cluster" patented technology

The invention provides a cell specific transcription factor regulatory network analysis method and a visualization platform, and relates to the technical field of bioinformatics, the method comprises the following steps: constructing a gene regulatory network through a GRNBoost2-cisTarget-AUCell-Cell GRN workflow based on a transcription factor in combination with a motif database; screening a direct regulation relationship in combination with the database, and calculating an activity score of a regulator in each cell; based on activity scores and cell type annotation results, grouping the single cell data by using a unified manifold approximation and projection (UMAP) dimensionality reduction method and a Leiden clustering algorithm, and displaying the following results through an interactive visualization tool: a cell clustering UMAP graph, performing color marking according to cell types; a UMAP graph and a heat map of transcription factor regulator activity; according to the visual map of the gene regulation and control network, transcription factors and target genes are distinguished through node shapes, and regulation and control relations are marked through line weights and colors. According to the invention, an accurate regulation and control network can be provided.

The invention discloses an animal single cell data cell type annotation method, which is characterized by comprising the following steps: collecting Bulk RNA-seq data of purified cell types in various tissues and organs of a specific animal, and integrating the Bulk RNA-seq data into a reference data set after preprocessing; the method comprises the following steps: acquiring single-cell RNA-seq original data, screening the single-cell RNA-seq original data to obtain high-quality cells, screening high-variation genes from the high-quality cells, processing the high-variation genes, extracting principal components, performing dimensionality reduction on the principal components, and performing cell clustering based on a dimensionality reduction result to obtain cell clusters; and calculating expression similarity between the cell clusters and the reference data set based on the high-variation genes, determining initial cell types of the cell clusters according to a similarity result, and carrying out iterative tuning on the initial cell types with similar scores to obtain a final cell type annotation result. The method provides efficient and accurate technical support for animal single cell research.

The invention discloses an auxiliary diagnosis system for renal transplantation rejection and typing thereof based on a peripheral blood immune cell map. Comprising a peripheral blood single cell acquisition unit, a mass spectrum flow cytometry unit, an original data preprocessing unit, a data conversion and normalization processing unit, a peripheral blood immune cell grouping acquisition unit, a peripheral blood immune cell map construction unit, a feature vector acquisition unit, a rejection prediction unit and a rejection typing prediction unit. According to the invention, a kidney transplantation two-stage diagnosis architecture is created for the first time; rejection detection and typing identification are realized through classification of an independently trained random forest binary classifier, and different TCMR / ABMR immune mechanisms are accurately matched; the traditional biopsy limitation is broken through, an auxiliary diagnosis scheme with high precision, low damage, moderate cost and typing identification capability is provided for the kidney transplantation rejection, and the method has important significance on timely discovery of the rejection of a kidney transplantation patient and improvement of prognosis.

The present disclosure relates to automated systems and methods adapted to quickly and accurately train a neural network to detect and / or classify cells and / or nuclei. The present disclosure also relates to automated systems and methods for using a trained cell detection and classification engine, such as one including a neural network, to classify cells within an unlabeled image.

A disaggregation system is disclosed. The system includes a reservoir containing a solution including multiple cell clusters. The system further includes a cluster processing system. The processing system includes at least one separation device having multiple separation elements spaced apart from each other, each separation element including an edge portion configured to apply a separation force to cell clusters that contact the edge portion. The processing system further includes one or more channels in fluid communication with the reservoir, the one or more channels configured to receive the solution from the reservoir and direct the solution to pass through a corresponding separation device, causing at least one cell cluster to contact the edge portion of one or more of the separation elements, causing the at least one cell cluster to disaggregate into at least two separate cell clusters.

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.

Facilitating energy aware multi-cell admission control in advanced communication networks is provided herein. A method includes, based on notification of a denial of an admission request by a first cell, facilitating a transmission of the admission request to a second cell and at least a third cell. The second cell and the third cell are neighbor cells of the first cell. The admission request can be received from a user equipment. The method also includes, based on receipt of an acceptance of the admission request from the second cell and the third cell, and based on a determination that a first utility of the second cell is higher than a second utility of at least the third cell, selecting the second cell as an admission cell for the user equipment. Further, the method includes facilitating admission of the user equipment to a cell cluster via the second cell.

The invention relates to the technical field of image data processing, and discloses a bone marrow biopsy image blurring removal method for cell structure recovery, which comprises the following steps: firstly, purifying reliable low-frequency information in a blurred image into a structure blueprint by applying low-pass filtering, and adaptively decoupling cell clusters in the structure blueprint; guiding a programmed algorithm to redraw an idealized cell structure by taking the statistical property of the structure blueprint as an instruction; according to the method, reliable low-frequency information in the image is used as a control instruction, and high-frequency details are generated in a forward construction mode, so that the risk that an error structure is introduced due to guess in a traditional repairing method is avoided, and through an internal verification correction mechanism, the error structure is effectively corrected, and the error correction accuracy is improved. Self-adaptive processing of cell clusters and artifacts is realized, and logic integrity and final reliability of a reconstruction result in biology are guaranteed.

Various solutions for cluster-based measurement prediction in mobile communications are described. A user equipment (UE) may perform a first measurement on a first cell cluster. The UE may obtain first predicted results associated with a second cell cluster based on a first artificial intelligence (AI) or machine learning (ML) based model and first measurement results of the first measurement. The UE may determine that it moves from the first cell to a second cell. The first predicted results are associated with the first cell and the second cell in an event that the first cell and the second cell belong to the second cell cluster. Accordingly, no AI or ML model update is needed for UE movement between cells belonging to the same cluster, thus reducing the overhead.

The invention provides a cell switching method, a base station and a computer storage medium, and the method comprises the steps: sending a switching preparation instruction to a switching request entity according to a current cell switching mode of user equipment, and enabling the switching request entity to carry out the cell switching preparation work; in response to receiving the switching preparation response message, sending source side data of the user equipment to the switching request entity, and establishing data transmission between the user equipment and the target cell by the switching request entity; based on the fact that a plurality of cells of the same cell cluster are located in the same frequency layer and configured with the same virtual cell identifier, non-inductive switching is achieved; and the source cell does not need to carry out data interaction related to inter-cell switching preparation work with the user equipment and does not need to send a switching command to the user equipment, and meanwhile, the user equipment continuously carries out data transmission in the inter-cell switching process, so that the influence of interruption of UE service transmission delay is reduced.

One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

The application discloses a method for preserving sugarcane germplasm resources in vitro, and belongs to the technical field of sugarcane germplasm preservation. The method comprises the following steps: taking a sugarcane tender leaf sheath as an explant, inducing and culturing callus, obtaining embryogenic cell clusters, and cryopreserving the obtained embryogenic cell clusters; the culture medium for the callus induction culture and the proliferation culture both contain mannitol and corn silk extract; the corn silk extract is an alcohol extract of corn silk. By adding mannitol and corn silk extract in the callus induction culture medium and the proliferation culture medium, the browning of the explant in the process of sugarcane in vitro tissue culture is effectively alleviated, the light condition is optimized, the activity of the embryogenic callus cell clusters is improved, the generation of sugarcane in vitro regenerated seedlings is promoted, and the survival rate of the regenerated seedlings after transplanting is improved.

A method for establishing cell clusters in a grid of a plurality of cells for receiving cell contents may include, dependent on the cell contents contained in the plurality of cells, forming at least one cell cluster out of at least two cells of the plurality of cells. The method may further include updating a completed establishment of cell clusters only locally in a region by at least one cell for which updated cell contents are present.

The subject of the application is a method for replenishing goods and managing the content and arrangement of goods in an iZMS automated store warehouse with a cell cluster structure applicable to the logistics of goods in retail chains and e-commerce, in the field of loading, storing, and issuing collected goods. The method for replenishing goods and managing the content and arrangement of goods in an iZMS automated store warehouse, comprising an IT system and a database, as well as a set of shelves with drawers and automatic, electronically controlled manipulators with grippers for appropriate goods, is characterized in that in the IT system of the iZMS automated store warehouse, for each warehouse cell comprising goods grouped in shelves, based on a sales analysis based on counting sales transactions of given goods in a given period and a forecasted model behavior established by the analysis systemCustomer demand as a function of the time horizon covering a specific season related to the designated quantitative limits for storage cell fill, critical supply quantitative minimum and maximum fill levels for these storage cells are determined. At the same time, the minimum quantitative supply limits for the need to fill these storage cells with goods are established. The fill level of specific storage cells depends on the physical parameters of the goods to which the storage cell is adapted, and the goods are grouped into subsets comprising goods and their corresponding containers and slots, occurring in a series of sizes adapted to the size of the goods handled, and corresponding collective packaging, baskets, drawers, and trays, appearing in the iZMS system in a single dimension selected for the specific industry, defining the subsets as a product-container-tray, product-slot-drawer correlation.Goods-slot-basket, goods-basket, so that at the goods identification and picking station, the monitor displays information to the operator based on the established limit value for the filling of a specific storage cell, consistent with the forecast for the selected time horizon, regarding the need to replenish goods, along with a list and quantity of goods, and a definition of the subset for each of the goods specific to each cell. Each cell is loaded with a loading rack adapted to the specific collective packages for the said loading rack and the established subset. Additionally, during loading / replenishing of the loading rack, each item is automatically identified using cameras and / or a graphic code scanner and an image analysis module, while simultaneously measuring the weight of the empty and successively filled collective package, while simultaneously recording and counting the items placed in the rack.The paired product(s) is then linked and verified with the defined subset, with a final weight measurement of the full bulk package, along with position data after placing it in the loading rack, which is then loaded into its defined cell. Each cell is loaded by replacing the loaded loading rack with an automatically prepared empty loading rack under the control of the IT system. Finally, information about the weight, quantity of goods, and their location in a specific bulk package, along with defined details about the subset and the location in the loading rack loaded into a specific cell, is saved and synchronized with the IT system. The purpose is to update the data on goods located in each iZMS cell, which, with ongoing sales analysis by the IT system, closes the loop managing the iZMS content.The automated store warehouse shown in the figure is also subject to notification.

A cell group division method based on overburden remodeling coordination belongs to the field of cell group division in single-cell RNA sequencing data analysis, and comprises the following steps: firstly, constructing cell multi-fractal dimension characteristics for single-cell RNA sequencing data so as to better utilize different fractal dimension characteristic information; and then multi-fractal-dimension low-dimensional representation is extracted. Secondly, extracting multi-fractal-dimension fusion representation, multi-fractal-dimension remodeling representation and cross-fractal-dimension prediction representation by adopting an obstacle covering mechanism so as to improve the robustness of fractal-dimension remodeling; and optimizing the initial division loss to obtain the initial division of the cells. Then, a confidence weight is obtained based on the remodeling error, the division entropy, and the suspension to reduce the negative effects of the low-quality fractal dimension data. And the consistency and coordination of cell group division are ensured by minimizing the division loss target, and a final cell group division result is obtained. Compared with other methods, the cell group division accuracy is higher, and the performance is more stable.

By swinging a culture vessel before aspiration removal of a medium, a centrally dense state (83) of the cell population is formed. In the centrally dense state (83), a cell cluster (84) is separated from an outlet port (58a). After aspiration removal of the medium, a new medium is introduced into the culture vessel. After the introduction of the new medium, an overall dispersed state of the cell population is formed.

Provided herein are methods, compositions, systems, kits and uses for integrated, simultaneous spatial multiomic analyses of tissues and samples including chromatin accessibility epigenomic analysis, RNA expression transcriptomic analysis, cell cluster and cell lineage analysis, transcription factor motif analysis, extrachromosomal DNA analysis and mitochondrial DNA analysis.

This invention belongs to the field of organoid culture technology, specifically involving BCL2 Application of genes in 3D cluster culture of stem cell-derived organoids. Starting on day 4 of bovine embryonic stem cell cluster culture, the cell cluster edges became indistinct, cells began to die and darken, and the cell clusters began to diffuse. By day 7, the cell cluster survival rate was only 14.2%. This invention utilizes the overexpression of genes in bovine embryonic stem cells... BCL2 Following gene expression, the cells were cultured in clusters using the same method. The overexpressed cell clusters showed clear edges, and their volume increased continuously with the number of culture days. Even on day 7, clear cell cluster edges were still observable, and no diffuse cell death was observed; the survival rate remained high at 85.54%. BCL2 Genes can improve the survival rate of early cultured cell clusters of organoids, which is of great significance for the normal morphogenesis and functional maturation of organoids and can greatly improve the efficiency of organoid preparation.

The invention discloses a PARP inhibitor sensitivity prediction method based on a specific cell cluster and application. The method comprises the following steps: acquiring an ovarian cancer tumor tissue sample of a subject; detecting whether a specific cell cluster exists in the sample or not, and determining the abundance of the specific cell cluster, wherein the specific cell cluster comprises an IFN epithelial cell cluster CN12 taking an IFN tumor cell as a center and / or a TAM enrichment cluster CN29 taking an M2 type tumor related macrophage as a center; according to the detection result, predicting according to at least one of the following rules: if the CN12 abundance before treatment is greater than 0.8%, the CN29 abundance before treatment is greater than 32.7%, the CN12 abundance after treatment is greater than 0.009%, the CN29 abundance after treatment is greater than 2.9%, or the SPP1 cell density before treatment is greater than 1.7%, predicting that the drug resistance is not sensitive or easily generated. The prediction model is established from a cell cluster space tissue level, and the method has the advantages of high prediction accuracy, flexible sample requirements, high operability and the like.

The application relates to the field of artificial intelligence, in particular to a cross-modal semantic alignment method of a multi-modal LLM-RAG local knowledge base, which comprises the following steps: S1, constructing a cross-modal semantic cell dynamic recombination base, which is used for disassembling original semantic information of various heterogeneous modes such as text, image and audio into the smallest independently representable semantic cells. The application disassembles various heterogeneous modes such as text, image and audio into semantic cells with mode identification, semantic weight and correlation threshold by constructing the cross-modal semantic cell dynamic recombination base, combines local data dynamic recombination iteration, solves the problem that the prior art ignores the structural difference of mode embedding space and is not suitable for sparse local data, builds a three-layer causal graph of mode-semantic-scene by means of a predictive distribution calibration mechanism with causal perception, locates the root of data distribution change and adjusts the cell cluster rule in combination with time series data prediction, and avoids semantic misalignment caused by data distribution deviation.

The present invention relates to a composition for the prevention or treatment of cisplatin-resistant cancer comprising an inhibitor of DUSP23 expression or activity as an active ingredient. It was found that DUSP23 expression is upregulated in cell clusters exhibiting characteristics of cancer stem cells, and that knocking down DUSP23 significantly reduces cell cluster formation as well as decreases the expression of SOX2, a major stem cell marker. Furthermore, DUSP23 expression was increased in lung cancer cells that had developed cisplatin resistance, and it was confirmed that the downregulation of DUSP23 inhibits self-renewal and invasive capabilities during the induction of cancer cell death. In addition, it was confirmed that DUSP23 plays an important role in promoting cancer stem cell characteristics through SOX2 expression. In conclusion, it has been shown that targeting DUSP23 can be a promising therapeutic strategy to overcome anticancer drug resistance in lung cancer, and the composition of the present invention is expected to be usefully utilized as a treatment for lung cancer cells with cisplatin resistance.

Embodiments of the present application relate to the field of communication technology, and disclose a wireless network optimization method, device and equipment, and a computer storage medium. The method comprises the following steps: determining a traffic matrix of a cell cluster in a preset time period; the traffic matrix comprises intra-cell traffic of each cell in the cell cluster and migration traffic from each cell to other cells in the cell cluster; determining a network performance characteristic matrix of a wireless network in which the cell cluster is located; determining total consumed traffic of the cell cluster according to the network performance characteristic matrix and the traffic matrix; taking the minimum total consumed traffic as a target and the sum of all intra-cell traffics in the cell cluster in the preset time period as a boundary condition, iteratively solving the network performance characteristic matrix to obtain an optimal solution; and adjusting the wireless network according to the optimal solution. In the above manner, the embodiments of the present application improve the efficiency and accuracy of wireless network optimization.

User equipment may include a transmitter and a receiver coupled to an antenna to enable the user equipment to transmit and receive user data with a base station of the wireless network. However, the user equipment may perform power-consuming searches to determine a base station for connection. Furthermore, the connection may be affected by blockages and transitions during mobility scenarios. As such, it may be beneficial for the user equipment to implement mobility procedures. For example, the user equipment may form links with multiple base stations of a cell cluster for transitioning. In another example, the wireless network may generate a map with locations of base stations and beam characteristics for the user equipment to determine coverage areas and decrease a number of transitions. Still in another example, the user equipment may receive blockage information to predict a blockage and implement mobility procedures to maintain wireless service during a blockage.

Device (1), and method for manufacturing the same, for detecting the growth of a three-dimensional cell cluster (C), comprising: a substrate (2) having a support surface (21); a plurality of electrodes (3) arranged according to a predetermined spatial distribution, for detecting an electrical potential in the presence of a three-dimensional cell cluster (C); a plurality of three-dimensional scaffolds (4), comprising at least a first scaffold (41), arranged at a respective measuring electrode (31), and at least a second scaffold (42), arranged at a respective reference electrode (32), wherein the at least one first scaffold (41) has a proliferation conformation, adapted to promote the growth of the three-dimensional cell cluster (C), and the at least one second scaffold (42) has an inhibition conformation adapted to prevent the growth of the three- dimensional cell cluster (C).

The application discloses a kind of multi-cell cluster parallel energy storage system control circuit and its control method, wherein, energy storage battery cluster parallel control circuit includes: N energy storage battery cluster, wherein N is positive integer;N energy storage battery cluster management module, the energy storage battery cluster management module is one-to-one with the energy storage battery cluster, and the energy storage battery cluster management module is used to collect the state information of its corresponding energy storage battery cluster;System management controller is used to issue control signal according to the summary N energy storage battery cluster state information, to make the battery cluster management module control its corresponding energy storage battery cluster;DC-DC converter is used to perform charging / discharging work according to the control signal.This circuit can be realized in energy storage system internal energy storage battery cluster between by the way of energy transmission realizes mutual complement of energy and finally reaches the consistency of state, prevents the high surge circulating current impact between parallel battery cluster, ensures the safety and reliability of multi-cell cluster parallel.

System (1000) comprising a component (10) and a coating device (100) for applying a coating medium to the component (10), the coating device (100) comprising a detection device (102) configured to detect at least one geometric property (h) of the component (10), and at least one coating nozzle (104) movable relative to the component (10), configured to coat only selective areas of a component surface of the component (10) with the coating medium depending on the at least one geometric property (h) and depending on at least one predetermined, component-dependent coating criterion based on the at least one geometric property (h), wherein the component (10) comprises a cell cluster (16) with several energy storage cells (18), a cell contacting device (20) and a summation current connector (21), wherein the coating device (100) is configured toonly the cell shoulder areas (22) of the energy storage cells (18) are coated with the coating medium, wherein after coating the cell contacting device (20) and the summation current connector (21) are free of coating medium.

The embodiment of the application provides an antenna weight optimization method and device, comprising: collecting reference signal receiving power (RSRP) reported by each user equipment in a target area, performing cluster division on cells in the target area according to the RSRP to obtain a plurality of cell clusters, and optimizing antenna weights of each cell cluster according to user equipment distribution in the target area. Through the application, the problem that user distribution is difficult to accurately obtain under high frequency because high-frequency product beam configuration depends on simulation calculation or experience of network planning and optimization personnel is solved, and then accurate user distribution is obtained, so that network coverage and efficiency of the whole optimization area are optimized.

Circulating cell clusters found in subjects suffering from cancer, autoimmune disorders, infections, or other diseases may be intercepted or disrupted by filtering them with in vivo or in vitro devices and, in some cases, exposing them to substances that reduce intercellular adhesion, such as enzymes.

A cell cluster embedding box comprises a box body and a box cover, the box body is provided with a first concave cavity, a plurality of first through holes are formed in the bottom in the first concave cavity, the box cover comprises a cover plate and a box dam arranged on the cover plate, a second concave cavity is formed in the box dam, and after the box cover is matched with the box body, the box dam is inserted into the first concave cavity. A plurality of second through holes are formed in the cover plate in the second concave cavity; first weighing paper is arranged at the bottom in the first concave cavity, second weighing paper is arranged at the bottom in the second concave cavity, and a fixing piece used for pressing the first weighing paper and the second weighing paper is arranged between the box body and the box cover. The weighing paper can prevent the cell mass from falling out through the through hole in the embedding box after the cell mass is dehydrated and becomes small, but the dehydration operation of the cell mass is not influenced; the weighing paper is tightly pressed by the fixing piece, so that the weighing paper is tightly attached to the bottom, and the weighing paper can block the through hole and does not influence the space in the embedding box.

The utility model discloses a multi-cell cluster parallel pre-charge energy storage device and cascade energy storage system, at least comprising a first energy storage module and a second energy storage module, a second switch member and a first resistor member are connected in series to form a first series branch, and a first switch member and the first series branch are connected in parallel to form at least part of a first buffer parallel branch. The fourth switch piece and the second resistor piece are connected in series to form a second series branch, the third switch piece and the second series branch are connected in parallel to form at least part of a second buffer parallel branch, and the head end of the first buffer parallel branch is used for being connected with a converter device. The tail end of the first buffer parallel branch is connected with the head end of the first battery cluster and the head end of the second buffer parallel branch, the tail end of the second buffer parallel branch is connected with the head end of the second battery cluster, and the tail end of the first battery cluster is connected with the tail end of the second battery cluster; on the basis that original functions are not affected, the structure is optimized, and the arrangement cost is reduced.