88 results about "Cell cluster" patented technology

A disaggregation system is disclosed. The system includes a reservoir containing a solution including multiple cell clusters. The system further includes a cluster processing system. The processing system includes at least one separation device having multiple separation elements spaced apart from each other, each separation element including an edge portion configured to apply a separation force to cell clusters that contact the edge portion. The processing system further includes one or more channels in fluid communication with the reservoir, the one or more channels configured to receive the solution from the reservoir and direct the solution to pass through a corresponding separation device, causing at least one cell cluster to contact the edge portion of one or more of the separation elements, causing the at least one cell cluster to disaggregate into at least two separate cell clusters.

Facilitating energy aware multi-cell admission control in advanced communication networks is provided herein. A method includes, based on notification of a denial of an admission request by a first cell, facilitating a transmission of the admission request to a second cell and at least a third cell. The second cell and the third cell are neighbor cells of the first cell. The admission request can be received from a user equipment. The method also includes, based on receipt of an acceptance of the admission request from the second cell and the third cell, and based on a determination that a first utility of the second cell is higher than a second utility of at least the third cell, selecting the second cell as an admission cell for the user equipment. Further, the method includes facilitating admission of the user equipment to a cell cluster via the second cell.

One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

A method for establishing cell clusters in a grid of a plurality of cells for receiving cell contents may include, dependent on the cell contents contained in the plurality of cells, forming at least one cell cluster out of at least two cells of the plurality of cells. The method may further include updating a completed establishment of cell clusters only locally in a region by at least one cell for which updated cell contents are present.

The subject of the application is a method for replenishing goods and managing the content and arrangement of goods in an iZMS automated store warehouse with a cell cluster structure applicable to the logistics of goods in retail chains and e-commerce, in the field of loading, storing, and issuing collected goods. The method for replenishing goods and managing the content and arrangement of goods in an iZMS automated store warehouse, comprising an IT system and a database, as well as a set of shelves with drawers and automatic, electronically controlled manipulators with grippers for appropriate goods, is characterized in that in the IT system of the iZMS automated store warehouse, for each warehouse cell comprising goods grouped in shelves, based on a sales analysis based on counting sales transactions of given goods in a given period and a forecasted model behavior established by the analysis systemCustomer demand as a function of the time horizon covering a specific season related to the designated quantitative limits for storage cell fill, critical supply quantitative minimum and maximum fill levels for these storage cells are determined. At the same time, the minimum quantitative supply limits for the need to fill these storage cells with goods are established. The fill level of specific storage cells depends on the physical parameters of the goods to which the storage cell is adapted, and the goods are grouped into subsets comprising goods and their corresponding containers and slots, occurring in a series of sizes adapted to the size of the goods handled, and corresponding collective packaging, baskets, drawers, and trays, appearing in the iZMS system in a single dimension selected for the specific industry, defining the subsets as a product-container-tray, product-slot-drawer correlation.Goods-slot-basket, goods-basket, so that at the goods identification and picking station, the monitor displays information to the operator based on the established limit value for the filling of a specific storage cell, consistent with the forecast for the selected time horizon, regarding the need to replenish goods, along with a list and quantity of goods, and a definition of the subset for each of the goods specific to each cell. Each cell is loaded with a loading rack adapted to the specific collective packages for the said loading rack and the established subset. Additionally, during loading / replenishing of the loading rack, each item is automatically identified using cameras and / or a graphic code scanner and an image analysis module, while simultaneously measuring the weight of the empty and successively filled collective package, while simultaneously recording and counting the items placed in the rack.The paired product(s) is then linked and verified with the defined subset, with a final weight measurement of the full bulk package, along with position data after placing it in the loading rack, which is then loaded into its defined cell. Each cell is loaded by replacing the loaded loading rack with an automatically prepared empty loading rack under the control of the IT system. Finally, information about the weight, quantity of goods, and their location in a specific bulk package, along with defined details about the subset and the location in the loading rack loaded into a specific cell, is saved and synchronized with the IT system. The purpose is to update the data on goods located in each iZMS cell, which, with ongoing sales analysis by the IT system, closes the loop managing the iZMS content.The automated store warehouse shown in the figure is also subject to notification.

Provided herein are methods, compositions, systems, kits and uses for integrated, simultaneous spatial multiomic analyses of tissues and samples including chromatin accessibility epigenomic analysis, RNA expression transcriptomic analysis, cell cluster and cell lineage analysis, transcription factor motif analysis, extrachromosomal DNA analysis and mitochondrial DNA analysis.

This invention belongs to the field of organoid culture technology, specifically involving BCL2 Application of genes in 3D cluster culture of stem cell-derived organoids. Starting on day 4 of bovine embryonic stem cell cluster culture, the cell cluster edges became indistinct, cells began to die and darken, and the cell clusters began to diffuse. By day 7, the cell cluster survival rate was only 14.2%. This invention utilizes the overexpression of genes in bovine embryonic stem cells... BCL2 Following gene expression, the cells were cultured in clusters using the same method. The overexpressed cell clusters showed clear edges, and their volume increased continuously with the number of culture days. Even on day 7, clear cell cluster edges were still observable, and no diffuse cell death was observed; the survival rate remained high at 85.54%. BCL2 Genes can improve the survival rate of early cultured cell clusters of organoids, which is of great significance for the normal morphogenesis and functional maturation of organoids and can greatly improve the efficiency of organoid preparation.

The invention discloses a PARP inhibitor sensitivity prediction method based on a specific cell cluster and application. The method comprises the following steps: acquiring an ovarian cancer tumor tissue sample of a subject; detecting whether a specific cell cluster exists in the sample or not, and determining the abundance of the specific cell cluster, wherein the specific cell cluster comprises an IFN epithelial cell cluster CN12 taking an IFN tumor cell as a center and / or a TAM enrichment cluster CN29 taking an M2 type tumor related macrophage as a center; according to the detection result, predicting according to at least one of the following rules: if the CN12 abundance before treatment is greater than 0.8%, the CN29 abundance before treatment is greater than 32.7%, the CN12 abundance after treatment is greater than 0.009%, the CN29 abundance after treatment is greater than 2.9%, or the SPP1 cell density before treatment is greater than 1.7%, predicting that the drug resistance is not sensitive or easily generated. The prediction model is established from a cell cluster space tissue level, and the method has the advantages of high prediction accuracy, flexible sample requirements, high operability and the like.

The application relates to the field of artificial intelligence, in particular to a cross-modal semantic alignment method of a multi-modal LLM-RAG local knowledge base, which comprises the following steps: S1, constructing a cross-modal semantic cell dynamic recombination base, which is used for disassembling original semantic information of various heterogeneous modes such as text, image and audio into the smallest independently representable semantic cells. The application disassembles various heterogeneous modes such as text, image and audio into semantic cells with mode identification, semantic weight and correlation threshold by constructing the cross-modal semantic cell dynamic recombination base, combines local data dynamic recombination iteration, solves the problem that the prior art ignores the structural difference of mode embedding space and is not suitable for sparse local data, builds a three-layer causal graph of mode-semantic-scene by means of a predictive distribution calibration mechanism with causal perception, locates the root of data distribution change and adjusts the cell cluster rule in combination with time series data prediction, and avoids semantic misalignment caused by data distribution deviation.

The present invention relates to a composition for the prevention or treatment of cisplatin-resistant cancer comprising an inhibitor of DUSP23 expression or activity as an active ingredient. It was found that DUSP23 expression is upregulated in cell clusters exhibiting characteristics of cancer stem cells, and that knocking down DUSP23 significantly reduces cell cluster formation as well as decreases the expression of SOX2, a major stem cell marker. Furthermore, DUSP23 expression was increased in lung cancer cells that had developed cisplatin resistance, and it was confirmed that the downregulation of DUSP23 inhibits self-renewal and invasive capabilities during the induction of cancer cell death. In addition, it was confirmed that DUSP23 plays an important role in promoting cancer stem cell characteristics through SOX2 expression. In conclusion, it has been shown that targeting DUSP23 can be a promising therapeutic strategy to overcome anticancer drug resistance in lung cancer, and the composition of the present invention is expected to be usefully utilized as a treatment for lung cancer cells with cisplatin resistance.

Embodiments of the present application relate to the field of communication technology, and disclose a wireless network optimization method, device and equipment, and a computer storage medium. The method comprises the following steps: determining a traffic matrix of a cell cluster in a preset time period; the traffic matrix comprises intra-cell traffic of each cell in the cell cluster and migration traffic from each cell to other cells in the cell cluster; determining a network performance characteristic matrix of a wireless network in which the cell cluster is located; determining total consumed traffic of the cell cluster according to the network performance characteristic matrix and the traffic matrix; taking the minimum total consumed traffic as a target and the sum of all intra-cell traffics in the cell cluster in the preset time period as a boundary condition, iteratively solving the network performance characteristic matrix to obtain an optimal solution; and adjusting the wireless network according to the optimal solution. In the above manner, the embodiments of the present application improve the efficiency and accuracy of wireless network optimization.

Device (1), and method for manufacturing the same, for detecting the growth of a three-dimensional cell cluster (C), comprising: a substrate (2) having a support surface (21); a plurality of electrodes (3) arranged according to a predetermined spatial distribution, for detecting an electrical potential in the presence of a three-dimensional cell cluster (C); a plurality of three-dimensional scaffolds (4), comprising at least a first scaffold (41), arranged at a respective measuring electrode (31), and at least a second scaffold (42), arranged at a respective reference electrode (32), wherein the at least one first scaffold (41) has a proliferation conformation, adapted to promote the growth of the three-dimensional cell cluster (C), and the at least one second scaffold (42) has an inhibition conformation adapted to prevent the growth of the three- dimensional cell cluster (C).

The application discloses a kind of multi-cell cluster parallel energy storage system control circuit and its control method, wherein, energy storage battery cluster parallel control circuit includes: N energy storage battery cluster, wherein N is positive integer;N energy storage battery cluster management module, the energy storage battery cluster management module is one-to-one with the energy storage battery cluster, and the energy storage battery cluster management module is used to collect the state information of its corresponding energy storage battery cluster;System management controller is used to issue control signal according to the summary N energy storage battery cluster state information, to make the battery cluster management module control its corresponding energy storage battery cluster;DC-DC converter is used to perform charging / discharging work according to the control signal.This circuit can be realized in energy storage system internal energy storage battery cluster between by the way of energy transmission realizes mutual complement of energy and finally reaches the consistency of state, prevents the high surge circulating current impact between parallel battery cluster, ensures the safety and reliability of multi-cell cluster parallel.

System (1000) comprising a component (10) and a coating device (100) for applying a coating medium to the component (10), the coating device (100) comprising a detection device (102) configured to detect at least one geometric property (h) of the component (10), and at least one coating nozzle (104) movable relative to the component (10), configured to coat only selective areas of a component surface of the component (10) with the coating medium depending on the at least one geometric property (h) and depending on at least one predetermined, component-dependent coating criterion based on the at least one geometric property (h), wherein the component (10) comprises a cell cluster (16) with several energy storage cells (18), a cell contacting device (20) and a summation current connector (21), wherein the coating device (100) is configured toonly the cell shoulder areas (22) of the energy storage cells (18) are coated with the coating medium, wherein after coating the cell contacting device (20) and the summation current connector (21) are free of coating medium.

Circulating cell clusters found in subjects suffering from cancer, autoimmune disorders, infections, or other diseases may be intercepted or disrupted by filtering them with in vivo or in vitro devices and, in some cases, exposing them to substances that reduce intercellular adhesion, such as enzymes.

The utility model discloses a multi-cell cluster parallel pre-charge energy storage device and cascade energy storage system, at least comprising a first energy storage module and a second energy storage module, a second switch member and a first resistor member are connected in series to form a first series branch, and a first switch member and the first series branch are connected in parallel to form at least part of a first buffer parallel branch. The fourth switch piece and the second resistor piece are connected in series to form a second series branch, the third switch piece and the second series branch are connected in parallel to form at least part of a second buffer parallel branch, and the head end of the first buffer parallel branch is used for being connected with a converter device. The tail end of the first buffer parallel branch is connected with the head end of the first battery cluster and the head end of the second buffer parallel branch, the tail end of the second buffer parallel branch is connected with the head end of the second battery cluster, and the tail end of the first battery cluster is connected with the tail end of the second battery cluster; on the basis that original functions are not affected, the structure is optimized, and the arrangement cost is reduced.

The utility model provides a device for sorting and enriching, which comprises more than one sorting and enriching module and a power module, the separation and enrichment module comprises a separation and enrichment unit and a separation and enrichment unit, n sorting chips; a fluid inlet; a plurality of fluid outlets; in different sorting and enriching modules, N is independently an integer greater than or equal to 1; one or more sample storage containers; one or more buffer solution storage containers; two or more collection containers; the power module can enable fluid in the sample storage container and the buffer solution storage container to flow into the collection container through the sorting and enriching unit, and fluid in the collection container flows back to the cavity connected with the collection container. By means of the device, the cells and / or the cell clusters can be screened for multiple times, so that the sorting effect is improved, the sizes of the cells and / or the cell clusters collected in the collecting containers fall into the target size range more, and follow-up detection is facilitated.

The invention belongs to the technical field of CHO cell culture, and particularly relates to a multi-parameter collaborative optimization temperature control culture tank. The culture tank comprises a tank body, a stirring assembly, a ventilation assembly and a controller, the stirring assembly comprises a vertically-arranged main stirring paddle and a transversely-arranged auxiliary stirring paddle, the main stirring paddle and the auxiliary stirring paddle are each provided with a plurality of stirring blades with different lengths, and the stirring blades are matched with the arc-shaped inner wall of the tank body; the main stirring paddle can break bubbles to increase the oxygen dissolving rate, and the auxiliary stirring paddle can form uniform axial fluid in the tank, reduce cell clusters and nutrition gradient, reduce shear force, realize efficient mass and oxygen transfer and avoid damage to shear force sensitive CHO cells; and a central controller and various sensors are matched, so that the stirring rotating speed and the introduction proportion and rate of each gas can be synergistically regulated and controlled, the culture environment is dynamically maintained to be in an optimal state, and the efficient culture of CHO cells is assisted.

The invention discloses a user information query method and device. The user information query method comprises the following steps: acquiring original crawling data; generating a standardized identity cell set according to the original crawling data; cellular group co-evolution is carried out on the standardized identity cellular set, so that a convergence cellular cluster set is obtained; performing evolution result dynamic index mapping on the convergence cell cluster set to obtain a dynamic association index database; obtaining user request information; and querying from the dynamic association index database according to user request information so as to obtain a query result. According to the user information query method, an identity information leakage monitoring scene is taken as core guidance, core pain points in the aspects of multi-source adaptation, association precision, cross-source aggregation and the like in the prior art are solved, and the user information query efficiency is improved through original designs such as cellular modeling, scene rule binding and cross-source association fusion. The comprehensive improvement of the query integrity, accuracy, efficiency and scene adaptability is realized, and the overall advantages are remarkable.

This invention relates to the field of early screening technology for atherosclerotic coronary heart disease (CAD), and discloses an early screening system for CAD based on peripheral blood immune cell atlases. The system includes a peripheral blood single-cell acquisition unit, a mass spectrometry flow cytometry detection unit, a raw data conversion unit, a peripheral blood immune cell cluster acquisition unit, a subpopulation merging unit, a feature vector acquisition unit, and a classification acquisition unit. It enables early screening and diagnosis of CAD, offering advantages such as high accuracy, high sensitivity, speed, efficiency, and moderate cost. Furthermore, the analysis samples are derived from patient peripheral blood, exhibiting good operability, non-invasiveness, stability, and repeatability. This provides richer diagnostic indicators for early screening and diagnosis of CAD, helping clinicians to develop timely and effective treatment plans for patients.

The application discloses a frozen embedding block of islet cell-like cell clusters meeting spatiotemporal omics continuous section analysis, a method and application thereof, and belongs to the field of frozen embedding block preparation. In order to solve the problem of RNA / DNA integrity and visualization of islet cell clusters, the method comprises the following steps: adding a buffer solution containing bromophenol blue drop by drop to the aggregated cell clusters on the inner surface of the bottom of a silk cloth to dye the cell clusters; keeping the selected area of the silk cloth in contact with the frozen embedding reagent surface of the lower half groove in the loading groove of a first mold, placing the first mold on dry ice, and completely freezing the frozen embedding reagent of the lower half groove and the cell clusters on the surface thereof; placing the first mold with the upper half groove frozen embedding reagent added in the loading groove on dry ice, completely freezing the frozen embedding reagent of the upper half groove and the cell clusters on the surface thereof, and making the frozen embedding reagent of the upper half groove and the frozen embedding reagent of the lower half groove be integrated into the frozen embedding block, and taking out the frozen embedding block, wherein the cell clusters are distributed at the middle position on the height of the frozen embedding reagent block.

The invention relates to a cell fate determinant identification method and a related device, and belongs to the technical field of bioinformatics, the cell fate determinant identification method comprises the following steps: determining expression quantities of transcription factors and target genes in cells in single cell transcriptome sequencing data, and constructing a cell lineage; constructing a gene regulation and control model based on the relationship between the expression quantity of each transcription factor in each cell cluster in the cell lineage in the cells and the expression quantity of each target gene in the cells; constructing a target function according to the gene regulation model precision of each cell cluster, the gene regulation model sparseness of each cell cluster and the gene regulation model similarity between the adjacent cell clusters, and determining a regulation coefficient of a transcription factor in each cell cluster to a target gene based on the target function; and determining a differential transcription factor between adjacent cell clusters based on the regulation coefficient, and determining a cell fate determinant based on the differential transcription factor. According to the invention, the cell fate determinant can be accurately identified.

User equipment may include a transmitter and a receiver coupled to an antenna to enable the user equipment to transmit and receive user data with a base station of the wireless network. However, the user equipment may perform power-consuming searches to determine a base station for connection. Furthermore, the connection may be affected by blockages and transitions during mobility scenarios. As such, it may be beneficial for the user equipment to implement mobility procedures. For example, the user equipment may form links with multiple base stations of a cell cluster for transitioning. In another example, the wireless network may generate a map with locations of base stations and beam characteristics for the user equipment to determine coverage areas and decrease a number of transitions. Still in another example, the user equipment may receive blockage information to predict a blockage and implement mobility procedures to maintain wireless service during a blockage.

The application provides an inflammation hot spot area identification method and device, equipment and storage medium, relating to the technical field of spatial transcriptomics. The inflammation hot spot area identification method comprises: obtaining spatial transcriptome data; calculating the spatial inflammation score coefficient of each cell to be tested; screening out abnormal cells; performing clustering processing to obtain cell clusters; and determining the inflammation hot spot area in the tissue to be tested according to the spatial enrichment characteristics. The inflammation score coefficient is calculated by deeply coupling the spatial coordinates and the gene expression amount, and the real inflammation microenvironment of the cell is accurately restored. An abnormal baseline model is constructed in combination with the healthy control group data, an objective screening standard is established, background noise is effectively eliminated, and high-precision extraction of abnormal cells is realized. Further, through clustering and spatial enrichment characteristic analysis, the spatial boundary of the inflammation hot spot area is accurately determined, the defects of inaccurate evaluation and fuzzy boundary of the traditional technology are effectively overcome, and the identification accuracy is greatly improved.

Provided are a beam weight adjustment method, and an electronic device and a storage medium. The method comprises: acquiring any cell cluster within a preset region and at least one target operating parameter corresponding to the cell cluster, wherein the target operating parameter is an operating parameter corresponding to an uplink signal; adjusting the beam weight of each communication cell in the cell cluster on the basis of the at least one target operating parameter corresponding to the cell cluster, so as to obtain a target beam weight of each communication cell when the target operating parameter reaches the optimal; and updating the real-time beam weight of each communication cell on the basis of the target beam weight of each communication cell.

The embodiment of the invention discloses a gene classification method and device based on gene expression data and electronic equipment, and belongs to the technical field of bioinformatics and the field of data processing. The method comprises the following steps: determining expression values of genes in different cell clusters to obtain an expression value data set corresponding to the genes; determining a target expression value threshold value of the gene according to the expression value distribution condition in the expression value data set; and classifying the genes in the cell cluster based on the target expression value threshold. According to the embodiment of the invention, the distribution rule of the gene expression values can be utilized, the objectivity and accuracy of biological classification can be improved, meanwhile, fluctuation of the gene expression level caused by the batch effect can be effectively handled, and the consistency of gene classification is ensured.

A cell assembly (10) of a high-voltage storage device is presented. The cell assembly (10) has several cells (12), each cell (12) having a cell terminal (30) formed by a cell extension (28) of a first pole (24) and a second pole (26) forming a cell housing (34). The cell assembly (10) also includes connecting conductors (14) that electrically connect the cell terminals (30) and / or the second poles (26) to each other. Additionally, a short-circuit connection (42) is provided outside the cells (12) through which the cell terminal (30) and the second pole (26) are directly electrically connected when a predefined first reference temperature is exceeded.

A normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the defined set of clusters. Support Vector Machine (SVM) subroutines are employed to identify reference populations of interest by generating multidimensional boundary definitions. These boundary definitions may be used to identify reference populations to use in defining or refining a centroid line or a radius or radii defining a set of normal clusters, and to characterize and compare a test set of cells to the defined set of normal clusters.

The embodiment of the invention provides a cell PCI optimization method and device, equipment and a storage medium, and relates to the technical field of communication. The cell PCI optimization method comprises the following steps: detecting a target cell with demodulation interference in a target network, determining a co-coverage cell cluster comprising the target cell and a cell having a direct or indirect co-coverage relationship with the target cell, and optimizing the PCI of the cells in the co-coverage cell cluster by taking minimization of the PCI mode N conflict times in the co-coverage cell cluster as an optimization target. Based on the cell PCI optimization method and device, the equipment and the storage medium provided by the embodiment of the invention, lightweight optimization can be performed on the cell PCI, and the optimization efficiency is improved.