8 results about "Staining technique" patented technology

The utility model relates to the technical field of cell slide dyeing, and discloses a gun head transverse spacing adjusting mechanism of a cell slide dyeing machine, which comprises a guide rail, the inner wall of the guide rail is in threaded connection with a screw rod, the right side of the guide rail is fixedly connected with a positioning frame, and the right end of the screw rod is fixedly connected with a fixed disc I; a second fixing disc is fixedly connected to the right side of the first fixing disc, a plurality of transmission blocks are fixedly connected to the outer wall of the second fixing disc, an inner gear ring is slidably connected to the inner wall of the positioning frame, a sun gear is arranged on the inner wall of the inner gear ring, and a plurality of planetary gears are connected to the outer wall of the sun gear in a meshed mode. And the left side of the planetary gear is fixedly connected with a planetary carrier. According to the utility model, the telescopic rod is started to push the asynchronous motor to slide in the positioning frame, and the asynchronous motor is clamped with the transmission groove in the transmission disc on the outer wall of the transmission shaft II, so that the purpose of adjusting the fineness while transversely moving is achieved, the tablet production precision is improved, and the practicability is improved.

The utility model relates to the technical field of cell staining, and discloses a liquid transfer device of a cell slide-making staining machine, which comprises a machine body and a belt, a motor I is arranged at the front end of the right side of the middle-upper part of the inner wall of the machine body, belt wheels are arranged at the front end and the rear end of the right side of the middle-upper part of the inner wall of the machine body, and the belt wheel at the front side is fixedly connected to the output end of the motor I; the two belt wheels are in transmission connection through a belt, first threaded rods are fixedly connected to the left sides of the outer walls of the belt wheels, first threaded blocks are in threaded connection to the middles of the outer walls of the two first threaded rods, and a second motor is arranged at the rear end of the right side of the upper middle portion of the inner wall of the machine body. The first motor drives the front belt wheel to rotate, the rear belt wheel is driven by belt transmission to rotate at the same time, the belt wheels drive the first threaded rod to rotate, and the position and the moving path of the distributor can be adjusted by accurately controlling rotation parameters of the first motor and the second motor so as to meet the requirements of different cell slide dyeing.

The utility model belongs to the technical field of cell staining, and particularly relates to a cell staining device which comprises a working table, the top of the working table is fixedly connected with a supporting frame, and one side of the supporting frame is connected with a first motor. According to the cell dyeing device, the supporting frame, the first motor, the screw rod, the movable block, the flow dividing pipe, the dropper, the connecting pipe, the stirring barrel, the second motor, the stirring rod, the water inlet pipe and the water outlet pipe are matched for use, so that the device can dye a plurality of groups of cells when being used by a worker, and the working efficiency is improved when the worker uses the device. According to the cell staining device, the problems that a staining agent is layered after standing for a long time, a plurality of groups of cells cannot be stained, and the staining agent on the glass slide needs to be manually and uniformly smeared are solved, so that the staining agent is not layered after standing for a long time, and the plurality of groups of cells can be stained; and the staining agent on the glass slide can be automatically and uniformly smeared.

The invention discloses an intelligent evaluation method and system for an immunohistochemical staining result, and relates to the technical field of immunohistochemical staining, and the method comprises the steps of data generation, cell region data generation, classification data generation, spatial analysis result data generation and adaptive scoring and quality control. The consistency and comparability of data are guaranteed, and deviation caused by dyeing difference and image quality fluctuation is overcome; secondly, accurately identifying each cell region by a cell nucleus instance segmentation technology, and providing detailed region attributes; further through multi-modal spatial analysis, the spatial distribution mode of protein expression and the interaction between cells are expressed, and more comprehensive data are provided for pathological mechanism research; and the reliability of the analysis process and the credibility of the result are ensured by a self-adaptive scoring and quality control mechanism, so that the accuracy, efficiency and interpretability of immunohistochemical image analysis are remarkably improved.

The invention discloses a method for detecting sperm morphological anomaly through an improved Pasteur staining method, and relates to the technical field of morphological analysis, and the method comprises the following steps: carrying out staining treatment on a sperm smear by using the improved Pasteur staining method to obtain a training data set and a test data set; based on a U-Net network, designing a convolutional neural network VCA-Net applied to sperm morphological anomaly detection; based on the training data set and the test data set, performing model training and testing on the designed convolutional neural network VCA-Net to obtain a final convolutional neural network VCA-Net; and evaluating the final convolutional neural network VCA-Net by using the evaluation index to complete the anomaly detection of the sperm morphology by the improved Pasteur staining method. The problems that in the prior art, SMA automatic detection is difficult to achieve, and manual detection consumes time and labor and is large in workload and high in subjectivity under existing microscopic imaging are solved.

The utility model provides a biological cell dyeing device relates to cell dyeing technical field, including base, the base upper surface one side is provided with a plurality of layers horizontal placement's superposition board, the superposition board upper surface four corner all fixed mounting has the socket and superposition board lower surface is located in any socket just below position all fixed mounting has the plug -in post, through setting up superposition board, and through setting up first jack, second jack and third jack of different distribution size on superposition board, utilize the plugging of front fender insert piece and rear fender insert piece and first jack, second jack or third jack realizes the relative position adjustment, and it is convenient for placing different size's object glass on superposition board, and utilize the resistance of front fender insert piece and rear fender insert piece to object glass corner position can fix the position of object glass.

The utility model discloses an immunohistochemical staining control device, which belongs to the technical field of immunohistochemical staining, and adopts the technical scheme that the immunohistochemical staining control device comprises a device body, a sealing cover is arranged at the top of the device body, and six sliding frames are arranged in the device body; a plurality of protection mechanisms and a plurality of auxiliary mechanisms are arranged in the sliding frame; the protection mechanism comprises a placement frame, a limiting frame, a disinfection frame, a connecting pipe and a fixing plate, the bottom of the placement frame extends into the sliding frame and is in close contact with the interior of the sliding frame, the bottom of the limiting frame is magnetically attracted to the top of the placement frame, and the interior of the disinfection frame is fixedly connected with the interior of the placement frame. The problems that in the existing reagent distribution and sample treatment process, most immunohistochemical staining control devices disinfect reagents after staining and moving out, so that when the samples are moved, the risk of cross contamination exists among residual samples in the devices, and the accuracy of a detection result is influenced are solved.

The present application relates to the technical field of rapid immunohistochemistry, and particularly relates to a rapid immunohistochemical staining method for pleural effusion cytology. The method comprises the following steps: S1, taking the pleural effusion for processing to obtain cell sediment, drying the water of the cell group, and embedding; S2, placing the cell group on a freezing mold, wrapping with OCT embedding agent, and placing in a quick freezer to quickly freeze into a block, and then slicing; S3, taking several slices from the prepared cell group freezing block of S2, and using flowing water and PBS to clean the OCT embedding agent on the slices, and then placing the slices in a preheated citric acid buffer for medium-low fire repair; S4, after the slice repair is completed, using an OptiView DAB IHC Detection Kit to perform manual immunohistochemical staining on the slice. The rapid immunohistochemical staining technology for pleural effusion cytology established by the present application can obtain consistent diagnostic results with conventional paraffin methods within 2 hours, and provides reliable support for intraoperative rapid cytological pathological diagnosis.