22 results about "Staining technique" patented technology

Techniques for implementing image normalization for multispectral fluorescence microscopy and virtual staining are disclosed. In an example method, a computing device receives, from an imaging device of a first imaging device type, a first autofluorescence image of a first tissue sample, the first autofluorescence image including one or more imaging channels. The computing device determines one or more normalization parameters for a first channel of the one or more imaging channels, the one or more normalization parameters for the first channel based on a first relationship between the first imaging device type and a second imaging device type, the second imaging device type being different from the first imaging device type. The computing device applies the one or more normalization parameters for the first channel to the first channel of the first autofluorescence image. The computing device outputs the normalized first channel of the first autofluorescence image.

The invention relates to the technical field of immunohistochemical staining, and discloses an automatic scoring method and system for immunohistochemical staining results. The method comprises the following steps: performing spectral signal intelligent deconvolution processing on a multi-immunohistochemical staining image to obtain target image data; performing multi-scale context modeling on the target image data to obtain a sub-region segmentation mask; performing depth map feature extraction on the target image data based on the subregion segmentation mask to obtain a multi-dimensional image feature vector; inputting the multi-dimensional image feature vector into a mixed deep learning recognition model to carry out marker intelligent recognition to obtain a multi-marker expression state recognition result; collaborative scoring is carried out based on the multiple marker expression state recognition result, a personalized unified scoring value is obtained, cell heterogeneity analysis is carried out on the personalized unified scoring value, and an intelligent scoring report is generated. According to the invention, the problem of spectrum crosstalk in multiple staining is effectively solved, and high-precision intelligent identification of multiple immunohistochemical markers is realized.

The utility model relates to the technical field of cell slide dyeing, and discloses a gun head transverse spacing adjusting mechanism of a cell slide dyeing machine, which comprises a guide rail, the inner wall of the guide rail is in threaded connection with a screw rod, the right side of the guide rail is fixedly connected with a positioning frame, and the right end of the screw rod is fixedly connected with a fixed disc I; a second fixing disc is fixedly connected to the right side of the first fixing disc, a plurality of transmission blocks are fixedly connected to the outer wall of the second fixing disc, an inner gear ring is slidably connected to the inner wall of the positioning frame, a sun gear is arranged on the inner wall of the inner gear ring, and a plurality of planetary gears are connected to the outer wall of the sun gear in a meshed mode. And the left side of the planetary gear is fixedly connected with a planetary carrier. According to the utility model, the telescopic rod is started to push the asynchronous motor to slide in the positioning frame, and the asynchronous motor is clamped with the transmission groove in the transmission disc on the outer wall of the transmission shaft II, so that the purpose of adjusting the fineness while transversely moving is achieved, the tablet production precision is improved, and the practicability is improved.

The invention discloses a DAB immune color development kit capable of enhancing color development performance and product stability and application of the DAB immune color development kit, and relates to the technical field of immunohistochemical staining. The DAB immune chromogenic kit provided by the invention comprises a DAB A solution, a DAB B solution and a DAB enhancer, the DAB A liquid comprises hydrochloric acid, 3, 3 '-diaminobenzidine and a specific polymer component; the DAB solution B comprises hydrogen peroxide, disodium ethylene diamine tetraacetate, tris (hydroxymethyl) aminomethane, hydrochloric acid and imidazole. A specific polymer component is introduced to form a compound with DAB, so that the rigidity of a precipitation product is remarkably enhanced, the problems of deformation and collapse caused by water loss in a dry sealing process are effectively solved, and the morphological characteristics of cell nucleuses are completely maintained. By optimizing the pH ratio system of the solution A and the solution B, the sensitivity and the dyeing strength of the chromogenic reaction are optimized, and the chromogenic strength and the sensitivity are further improved in cooperation with the copper sulfate enhancer. The kit is particularly suitable for nuclear antigen labeling items such as Ki-67 and the like, and is suitable for manual and automatic platforms.

The utility model relates to the technical field of cell staining, and discloses a liquid transfer device of a cell slide-making staining machine, which comprises a machine body and a belt, a motor I is arranged at the front end of the right side of the middle-upper part of the inner wall of the machine body, belt wheels are arranged at the front end and the rear end of the right side of the middle-upper part of the inner wall of the machine body, and the belt wheel at the front side is fixedly connected to the output end of the motor I; the two belt wheels are in transmission connection through a belt, first threaded rods are fixedly connected to the left sides of the outer walls of the belt wheels, first threaded blocks are in threaded connection to the middles of the outer walls of the two first threaded rods, and a second motor is arranged at the rear end of the right side of the upper middle portion of the inner wall of the machine body. The first motor drives the front belt wheel to rotate, the rear belt wheel is driven by belt transmission to rotate at the same time, the belt wheels drive the first threaded rod to rotate, and the position and the moving path of the distributor can be adjusted by accurately controlling rotation parameters of the first motor and the second motor so as to meet the requirements of different cell slide dyeing.

The present application relates to a kind of based on HE staining image tumor HER2 expression grading method.It includes: each target HE image block in target HE image block group is respectively loaded into IHC image generation model, to generate the IHC image block to be examined with current target HE image block in structure alignment state using IHC image model;Each IHC image block to be examined is loaded into multimodal semantic classification model, to use the multimodal semantic classification model to carry out expression grading processing, and the HER2 expression grade prediction information of current IHC image block to be examined is generated after grading processing.The present application can realize tumor HER2 expression grading prediction based on HE staining image, reduce the dependence on IHC staining technology, while improving the accuracy, stability and clinical availability of HER2 expression grading prediction.

The utility model belongs to the technical field of cell staining, and particularly relates to a cell staining device which comprises a working table, the top of the working table is fixedly connected with a supporting frame, and one side of the supporting frame is connected with a first motor. According to the cell dyeing device, the supporting frame, the first motor, the screw rod, the movable block, the flow dividing pipe, the dropper, the connecting pipe, the stirring barrel, the second motor, the stirring rod, the water inlet pipe and the water outlet pipe are matched for use, so that the device can dye a plurality of groups of cells when being used by a worker, and the working efficiency is improved when the worker uses the device. According to the cell staining device, the problems that a staining agent is layered after standing for a long time, a plurality of groups of cells cannot be stained, and the staining agent on the glass slide needs to be manually and uniformly smeared are solved, so that the staining agent is not layered after standing for a long time, and the plurality of groups of cells can be stained; and the staining agent on the glass slide can be automatically and uniformly smeared.

The invention relates to a hyper-fusion system IO time delay observation method and device based on an IO dyeing technology, and aims to solve the problems of inaccurate IO time delay observation and low efficiency in the prior art. The method comprises the steps that a user starts IO time delay observation and configures parameters through a UI configuration module; the IO dyeing module marks a unique mark on IO sent by the selected VM; the IO time delay capturing module captures IO carrying dyeing information at a key point of an IO life cycle and records key information; and the IO time delay analysis module obtains the total time delay of the IO by analyzing the IO track information generated by the IO capture module and backtracking the full life cycle of the IO, and decides whether to further backtrack the interlayer time delay and the intra-layer time delay of the IO full path or not according to the comparison between the total time delay and a configured time delay threshold value, and outputs the interlayer time delay and the intra-layer time delay to the UI interface. According to the method, the IO operation of the specific VM is accurately tracked through the IO dyeing technology, the key information of the IO operation is comprehensively obtained, the performance bottleneck is quickly positioned, and the observation efficiency is improved.

The invention discloses an intelligent evaluation method and system for an immunohistochemical staining result, and relates to the technical field of immunohistochemical staining, and the method comprises the steps of data generation, cell region data generation, classification data generation, spatial analysis result data generation and adaptive scoring and quality control. The consistency and comparability of data are guaranteed, and deviation caused by dyeing difference and image quality fluctuation is overcome; secondly, accurately identifying each cell region by a cell nucleus instance segmentation technology, and providing detailed region attributes; further through multi-modal spatial analysis, the spatial distribution mode of protein expression and the interaction between cells are expressed, and more comprehensive data are provided for pathological mechanism research; and the reliability of the analysis process and the credibility of the result are ensured by a self-adaptive scoring and quality control mechanism, so that the accuracy, efficiency and interpretability of immunohistochemical image analysis are remarkably improved.

The invention discloses a method for detecting sperm morphological anomaly through an improved Pasteur staining method, and relates to the technical field of morphological analysis, and the method comprises the following steps: carrying out staining treatment on a sperm smear by using the improved Pasteur staining method to obtain a training data set and a test data set; based on a U-Net network, designing a convolutional neural network VCA-Net applied to sperm morphological anomaly detection; based on the training data set and the test data set, performing model training and testing on the designed convolutional neural network VCA-Net to obtain a final convolutional neural network VCA-Net; and evaluating the final convolutional neural network VCA-Net by using the evaluation index to complete the anomaly detection of the sperm morphology by the improved Pasteur staining method. The problems that in the prior art, SMA automatic detection is difficult to achieve, and manual detection consumes time and labor and is large in workload and high in subjectivity under existing microscopic imaging are solved.

The utility model provides a biological cell dyeing device relates to cell dyeing technical field, including base, the base upper surface one side is provided with a plurality of layers horizontal placement's superposition board, the superposition board upper surface four corner all fixed mounting has the socket and superposition board lower surface is located in any socket just below position all fixed mounting has the plug -in post, through setting up superposition board, and through setting up first jack, second jack and third jack of different distribution size on superposition board, utilize the plugging of front fender insert piece and rear fender insert piece and first jack, second jack or third jack realizes the relative position adjustment, and it is convenient for placing different size's object glass on superposition board, and utilize the resistance of front fender insert piece and rear fender insert piece to object glass corner position can fix the position of object glass.

The invention relates to a kit and a method suitable for Golgi staining of large animals. The kit comprises: 1) an impregnation liquid, wherein the impregnation liquid is an AB liquid, and the AB liquid is an isovolumetric mixed liquid of 5 wt% potassium dichromate (liquid A) and 5 wt% mercuric chloride (liquid B); the solution C is 1.4 wt% potassium chromate solution, the color developing solution is a mixed solution of a solution D, a solution E and flushing fluid in a volume ratio of 1: 1: 1.8-1: 1: 2.5, and the solution D is a mixed solution of 25vol% stronger ammonia water and Milli-Q water; the solution E is a sodium thiosulfate pentahydrate aqueous solution; and the flushing fluid is Milli-Q water. Methods of Golgi staining of large animal brain tissue are also provided. According to the method, high-definition and quantitative-analysis staining results are provided for neuroscience research of the domestic dogs, and reference is provided for optimization of Golgi staining technologies of other large animals.

The application discloses a kind of virtual dyeing verification methods for autofluorescence and HE staining image registration, including marking paper module, slice buckle module, image acquisition module, image registration module, virtual dyeing module and verification output module.The virtual dyeing verification method for autofluorescence and HE staining image registration of the application introduces high-precision marking paper as registration reference, combines deep learning model with image processing algorithm, realizes the accurate registration of two modal images and virtual dyeing verification.The scheme aims to solve the registration error problem caused by tissue deformation or dyeing difference in traditional dyeing technology, ensure that virtual dyeing result and real dyeing image are highly consistent in space, and provide reliable basis for pathological diagnosis.

The utility model discloses an immunohistochemical staining control device, which belongs to the technical field of immunohistochemical staining, and adopts the technical scheme that the immunohistochemical staining control device comprises a device body, a sealing cover is arranged at the top of the device body, and six sliding frames are arranged in the device body; a plurality of protection mechanisms and a plurality of auxiliary mechanisms are arranged in the sliding frame; the protection mechanism comprises a placement frame, a limiting frame, a disinfection frame, a connecting pipe and a fixing plate, the bottom of the placement frame extends into the sliding frame and is in close contact with the interior of the sliding frame, the bottom of the limiting frame is magnetically attracted to the top of the placement frame, and the interior of the disinfection frame is fixedly connected with the interior of the placement frame. The problems that in the existing reagent distribution and sample treatment process, most immunohistochemical staining control devices disinfect reagents after staining and moving out, so that when the samples are moved, the risk of cross contamination exists among residual samples in the devices, and the accuracy of a detection result is influenced are solved.

The invention discloses a marker for predicting the risk of colorectal cancer lymphatic vessel invasion and application, and belongs to the technical field of tumor biomarker research. Aiming at the problem that the current diagnosis of lymphatic vessel invasion is limited by a dyeing technology, the number of tissue blocks and the experience and professional degree of pathologists, the invention discovers that PLR, D-dimer, CA19-9 and CA724 in peripheral blood laboratory indexes are related to lymphatic vessel invasion through clinical pathological feature analysis; a risk factor scoring system constructed based on PLR, D-dimer, CA199 and CA724 is an independent prediction factor of lymphatic vessel invasion. The risk factor score is combined with other common clinical pathological variables to construct a column graph model for predicting the lymphatic vessel invasion risk, diagnosis of a pathologist can be assisted, and a clinician can be guided to make a treatment strategy.

The invention provides an immunohistochemical staining device, and relates to the technical field of immunohistochemical staining. The cover plate is installed in the second limiting structure and provided with a cavity and a sealing piece located on the peripheral side of the cavity, and the sealing piece and the surface of the glass slide jointly seal the cavity. The cover plate is provided with a liquid inlet, and a plurality of different reagents enter the cavity from the liquid inlet and then are mixed so as to dye a human tissue slice sample. The elastic assembly is located on one side of the cover plate and abuts against the cover plate so as to clamp the cover plate. The ejector rods penetrate through the base and abut against the top end of the cover plate, and each ejector rod is located on the side, away from the elastic assembly, of the cover plate and is controlled to move up and down relative to the base so as to drive the top end of the cover plate to move up and down, so that a plurality of different reagents are fully mixed. According to the embodiment, mixing of different reagents is achieved through movement of the ejector rod and the cover plate, and compared with manual operation, the mixing uniformity and the dyeing uniformity and consistency can be improved, so that the dyeing quality is improved.

The invention relates to the technical field of medical research, in particular to a method for evaluating the invasive growth mode of hepatocellular carcinoma based on multiple pathological staining technologies, which comprises the following steps: taking liver tissue, pretreating a specimen, preparing a slice, and obtaining a specimen slice; dyeing the specimen sections by using hematoxylin-eosin dyeing, and analyzing the dyeing condition; the method comprises the following steps: respectively carrying out masson staining and specific immunohistochemical staining on a key junction region of a specimen section on the basis of a staining condition, and evaluating an invasive growth mode of hepatocellular carcinoma, so that the invasive growth mode of hepatocellular carcinoma is evaluated by carrying out multi-dimensional observation and analysis on the junction region of hepatocellular carcinoma tissue and multiple adjacent structures; three pathological technologies of basic morphology, fibrosis evaluation and specific cell localization immunohistochemical staining are combined, mutual verification is carried out, the limitation of a single technology is made up, and a new pathological basis is provided for accurately knowing the growth characteristics and invasion potential of hepatocellular carcinoma and formulating an individualized diagnosis and treatment strategy.

The present application relates to the technical field of rapid immunohistochemistry, and particularly relates to a rapid immunohistochemical staining method for pleural effusion cytology. The method comprises the following steps: S1, taking the pleural effusion for processing to obtain cell sediment, drying the water of the cell group, and embedding; S2, placing the cell group on a freezing mold, wrapping with OCT embedding agent, and placing in a quick freezer to quickly freeze into a block, and then slicing; S3, taking several slices from the prepared cell group freezing block of S2, and using flowing water and PBS to clean the OCT embedding agent on the slices, and then placing the slices in a preheated citric acid buffer for medium-low fire repair; S4, after the slice repair is completed, using an OptiView DAB IHC Detection Kit to perform manual immunohistochemical staining on the slice. The rapid immunohistochemical staining technology for pleural effusion cytology established by the present application can obtain consistent diagnostic results with conventional paraffin methods within 2 hours, and provides reliable support for intraoperative rapid cytological pathological diagnosis.

The application discloses a sodium cholate Golgi staining solution and a staining method for brain tissue staining. The sodium cholate Golgi staining solution is prepared by compounding a basic Golgi-cox staining solution and sodium cholate. The basic Golgi-cox staining solution is prepared by mixing 5% mercuric chloride solution, 5% potassium dichromate solution, 5% potassium chromate solution and deionized water in a volume ratio of 5:5:4:10, and then storing the mixture in dark for at least two days to obtain supernatant. The final concentration of the sodium cholate is 0.25-1 (w / v) %. The application introduces exogenous sodium cholate into the Golgi-cox staining solution for the first time, improves the classical Golgi-cox staining method, shortens the traditional staining period of several weeks to several days, lays a good foundation for studying the action mechanism of the Golgi-cox staining, and provides a simple, low-cost and stable staining technology support for various brain science neuron morphology research.

The utility model belongs to the technical field of pathological staining, and particularly relates to a staining device for pathological technology, which comprises a dewaxing staining machine, the dewaxing staining machine comprises a base, the top of the base is fixedly connected with a clamping seat, an inner cavity of the clamping seat is movably connected with a dye vat, and the back side of the top of the base is fixedly connected with a supporting rod; the motor is started to drive the blades to rotate, at the moment, the third pipeline begins to suck dimethyl benzene volatile gas in the shell, the volatile gas is sucked into the filter bin through the first pipeline, and the gas sucked into the filter bin is firstly subjected to basic filtration through the filter screen and then is subjected to deep filtration through the poison filter box; and finally, the liquid is discharged through the air outlet, so that the filtering effect is achieved, and the problems that in the dyeing process, a xylene solvent needs to be used when the tissue to be dyed is dewaxed, but xylene has volatility and is a toxic substance, and the health may be harmed due to long-term contact or inhalation of steam of the xylene are solved.

The application discloses a kind of tissue sample decoloring reagent and the method for processing tissue, and the component of decoloring reagent includes hydrogen peroxide, anhydrous ethanol, polyethylene glycol octylphenyl ether and ethylenediaminetetraacetic acid.The method for processing tissue described in the application includes the tissue sample to be processed is immersed in decoloring reagent processing.Compared with prior art, the application uses hydrogen peroxide as the main decoloring component, removes endogenous autofluorescence such as hemoglobin, lipofuscin etc. in the interior of animal tissue by using its strong oxidizing property, and simultaneously removes residual pigment by using ethylenediaminetetraacetic acid and anhydrous ethanol for auxiliary decoloring, polyethylene glycol octylphenyl ether improves tissue permeability and enhances molecular penetration capacity.The application is suitable for various animal tissue samples, can prepare animal tissue sample thick section in a short time, and under the condition that various staining techniques are compatible and the structure of preserved tissue is complete, endogenous autofluorescence in tissue is removed to the greatest extent.

The utility model relates to the technical field of cell staining, and discloses an integrated animal tissue staining rack set which comprises a protection box, a limiting rack is slidably connected to the interior of the protection box, two limiting frames are fixedly connected to the top of the limiting rack, sliding blocks are slidably connected to the interiors of the limiting frames, and the sliding blocks are fixedly connected to the interiors of the limiting frames. And a rotating block is rotationally connected to the interior of the sliding block, limiting arcs are fixedly connected to the two sides of the rotating block, a limiting rod is fixedly connected to the rear end of the sliding block, and a limiting assembly used for limiting parts is slidably connected to the top of the limiting frame. According to the utility model, the adaptation to the tissue slices with different sizes is realized, and the tissue slices can be properly placed in the limiting frame regardless of the sizes through the flexible matching mechanism, so that the universality of the device is improved, and the device can be widely applied to research scenes of different animal tissue slices.