The present application relates to the technical field of rapid
immunohistochemistry, and particularly relates to a rapid immunohistochemical
staining method for
pleural effusion cytology. The method comprises the following steps: S1, taking the
pleural effusion for
processing to obtain
cell sediment,
drying the water of the
cell group, and embedding; S2, placing the
cell group on a freezing mold, wrapping with OCT
embedding agent, and placing in a quick freezer to quickly freeze into a block, and then
slicing; S3, taking several slices from the prepared cell group freezing block of S2, and using flowing water and PBS to clean the OCT
embedding agent on the slices, and then placing the slices in a preheated
citric acid buffer for medium-low fire repair; S4, after the slice repair is completed, using an OptiView DAB IHC Detection Kit to perform manual immunohistochemical
staining on the slice. The rapid immunohistochemical
staining technology for
pleural effusion cytology established by the present application can obtain consistent diagnostic results with conventional paraffin methods within 2 hours, and provides reliable support for intraoperative rapid cytological
pathological diagnosis.