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Method for rapidly identifying cadmium sensitivity of rice by using fluorescent staining technology

A fluorescent dyeing and sensitivity technology, applied in the directions of fluorescence/phosphorescence, material analysis by optical means, measuring devices, etc., can solve the problems of poor accuracy, low efficiency and large error of rice cadmium sensitivity, and achieve short detection time, The effect of high efficiency and high work efficiency

Inactive Publication Date: 2021-09-17
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for quickly identifying rice cadmium sensitivity using fluorescent dyeing technology, which solves the problems of poor accuracy, large error and low efficiency of the existing method for detecting rice cadmium sensitivity

Method used

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  • Method for rapidly identifying cadmium sensitivity of rice by using fluorescent staining technology
  • Method for rapidly identifying cadmium sensitivity of rice by using fluorescent staining technology

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Embodiment 1

[0033] like Figure 1-2 As shown, the embodiments of the present invention provide a method for quickly identifying rice cadmium sensitivity using fluorescent staining technology, comprising the following steps:

[0034] S1. Coercion treatment

[0035] The detached leaves of different rice genotypes were cut and put into different test tubes, and the same amount of cadmium solution was added dropwise to the test tubes until the detached leaves were submerged. The detached leaves were soaked for 48 hours, and the detached leaves were treated in the cadmium solution. Stress treatment, mesophyll cells release a large amount of reactive oxygen species (ROS), under the catalysis of enzymes,

[0036] Active oxygen reacts with fluorescein to form a fluorescent compound;

[0037] S2. Microscope observation

[0038] Take out the detached leaves in the test tube and place them under a fluorescence microscope to observe the fluorescence intensity and distribution characteristics in th...

Embodiment 2

[0054] Such as Figure 1-2 As shown, the embodiments of the present invention provide a method for quickly identifying rice cadmium sensitivity using fluorescent staining technology, comprising the following steps:

[0055] S1. Coercion treatment

[0056] The detached leaves of different rice genotypes were cut and put into different test tubes, and the same amount of cadmium solution was added dropwise to the test tubes until the detached leaves were covered, and the detached leaves were soaked for 6 hours, and the detached leaves were treated in the cadmium solution Under stress treatment, mesophyll cells release a large amount of reactive oxygen species (ROS), and under the catalysis of enzymes, reactive oxygen species react with luciferin to form fluorescent compounds;

[0057] S2. Microscope observation

[0058] Take out the detached leaves in the test tube and place them under a fluorescence microscope to observe the fluorescence intensity and distribution characterist...

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Abstract

The invention provides a method for rapidly identifying cadmium sensitivity of rice by using a fluorescent staining technology, and relates to the technical field of rice breeding. The method for rapidly identifying the cadmium sensitivity of the rice by using the fluorescent staining technology comprises the following steps: S1, stress treatment; and S2, microscope observation. According to method, cadmium sensitivities of different genotypes of rice are intuitively and accurately judged by utilizing correlation between quantity of active oxygen generated by in-vitro leaves in cadmium stress environment and fluorescence intensity, and cadmium sensitivities of different genotypes are identified on basis of fluorescence intensity and distribution characteristics in rice leaves. According to the method, only a small number of rice leaves are needed, the method is not limited by the size of the rice, and the cadmium tolerance of the rice can be rapidly and accurately identified in a short time. The method has the advantages of being high in efficiency, good in repeatability, low in cost and the like, a large number of genotypes sensitive to cadmium tolerance are screened according to the leaf fluorescence intensity identification technology, the scale of field trials can be effectively reduced, and the method is worthy of being vigorously popularized.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for rapidly identifying cadmium sensitivity of rice using a fluorescent dyeing technique. Background technique [0002] Cadmium is the most widely distributed and most toxic heavy metal in the world, but rice itself has a strong ability to tolerate cadmium, even if it is planted in clean soil with a Cd content of less than 0.3 mg kg rice. Therefore, the country has invested a lot of money to support the breeding of rice genotypes with low cadmium accumulation. The cadmium content of rice is highly positively correlated with the cadmium tolerance of rice. Rice genotypes sensitive to cadmium generally have low cadmium accumulation characteristics. [0003] At present, the identification of cadmium-tolerant ability of rice genotypes is mainly done by comparing the differences among genotypes in the cadmium-stressed environment, such as the survival rate of rice seed...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6458G01N2021/6439
Inventor 刘仲齐张昕王常荣张长波黄永春
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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