196 results about "Fluorescence microscope" patented technology

The invention belongs to the field of molecular sieve material characterization, and particularly relates to a method for analyzing aluminum atom pairs in a ZSM-5 molecular sieve based on super-resolution fluorescence microscopic imaging, which comprises the following steps: activating a ZSM-5 molecular sieve sample; contacting the obtained sample with probe molecules; washing the ZSM-5 molecular sieve sample, and removing unbound or nonspecifically bound probe molecules; imaging the marked sample by using a super-resolution fluorescence microscope to obtain an image sequence containing a single fluorescent molecule emission event; processing the image sequence, determining a space coordinate of each fluorescence event, and reconstructing a super-resolution fluorescence image exceeding an optical diffraction limit; and based on the space coordinates of the fluorescent dots in the super-resolution fluorescence image, carrying out statistics to obtain the position, surface density or distribution information of the aluminum atom pairs in the ZSM-5 molecular sieve. According to the method disclosed by the invention, nanoscale spatial resolution, direct imaging and quantitative analysis of Al-pair in ZSM-5 (Zeolite Socony Mobil-5) can be realized.

The application belongs to the technical field of biology and specifically relates to a method for analyzing glycans on single extracellular vesicles in a fluid sample. The method uses magnetic nanoparticles to capture and manipulate EVs, based on the specific affinity of lectins for specific glycan structures, uses polydisperse microdroplets to separate the captured EVs, realizes local signal amplification in the droplets through enzymatic reaction, adopts fluorescence microscopy to collect images of the droplets, and thus realizes identification and analysis of the glycan components on single EVs. The preparation process of the microdroplets formed by the oscillating emulsification method of the application makes the entire detection process more simple and fast, does not require any microfluidic chip and special device, and can be completed in a centrifugal tube, and all the microdroplets are generated in parallel and quickly, and the amount of microdroplets can be flexibly expanded. Moreover, compared with the traditional ELISA, the method has high sensitivity, low detection limit and small sample consumption.

The invention relates to the technical field of aquatic animal cell culture, in particular to a rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus. Comprising the following steps: selecting healthy plectropomus leopardus for anesthesia; the method comprises the following steps: taking gonad tissues, performing sterile treatment, cutting, centrifugal washing and filtering, inoculating the gonad tissues into a culture medium for primary culture to obtain gonad cells emigrated from tissue blocks, performing trypsin-EDTA digestion and centrifugal washing, and treating the gonad cells with 1 * PBS containing polysorbate; after closing, antibody incubation and washing, the marking effect is verified through a fluorescence microscope, and specific enrichment of gonad somatic cells is achieved; and filtering and purifying the enriched fluorescence-labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescence-positive cells under a fluorescence visual field, transferring the fluorescence-positive cells into a culture dish containing a complete medium, inoculating the separated fluorescence-positive cells into the optimized complete medium, and carrying out purification culture, so that the cells grow in a wall-attached manner and stable passage is realized.

A light beam is imaged within a sample and images of the sample are generated based on light received from the sample in response to the light imaged within the sample. A wavefront modulating element modifies a wavefront of the received light and / or a wavefront of the light imaged within the sample. One or more known aberrations are introduced into at least one image of the sample and, based on at least two images of the sample, where the images include a raw image and at least one image that includes a known aberration, an aberration of a wavefront of light emitted from, and / or provided to, the sample is estimated. The wavefront modulating element is controlled to modulate the wavefront of light emitted from, and / or provided to, the sample, such that the estimated aberration of the wavefront of light emitted from, and / or provided to, the sample is reduced.

In various aspects, microscope systems are described, comprising or corresponding to optical microscopes, such as for example a transillumination microscope or a fluorescence microscope. The microscope systems may be used independently or configured with or integrated into another device or system, such as an imaging mass spectrometer or imaging mass cytometer. In various embodiments of this aspect, the microscope system is used to obtain one or more images of a sample, such as at a variety of different light collection wavelengths and / or for a variety of different illumination conditions. The microscope systems can employ various optical components and configurations allowing for high throughput imaging, such as components for imaging the sample at suitable resolutions with a wide field of view and / or for spatially separating different detection channel wavelengths from light output (e.g., transmitted or emitted light) from the sample.

The invention discloses a light field microscopic self-supervised denoising system and method fusing a physical process. The system comprises a light field imaging subsystem and a self-supervised denoising subsystem. The light field imaging subsystem is used for capturing multi-view light field original data of a sample; the self-supervised denoising subsystem is used for processing original data of a light field, preprocessing the original data of the light field in a training stage, and decomposing the original data into multi-view sub-images; the method comprises the following steps: constructing original data of a training set through two different self-supervision strategies, and then generating a self-supervision training data pair through RL deconvolution according to a physical optical process; and finally, a denoising model is obtained through 3D neural network training. In the reasoning stage, light field original data to be reconstructed are preprocessed and then subjected to RL deconvolution to generate input data to be denoised, the input data are input into the trained denoising model, and a reconstruction result is output. According to the invention, the problems of noise amplification, data dependence and poor universality of the light field fluorescence microscope are effectively overcome.

The invention discloses a rapid bacterium detection and counting method based on thiohydracrylic acid quantum dot fluorescence labeling, and the labeling method comprises the following steps: carrying out water-soluble modification on oil-phase quantum dots to obtain water-soluble quantum dot solids, uniformly dispersing the water-soluble quantum dot solids in water, and adjusting the pH value to 12-13 to obtain a water-phase quantum dot solution, the method for water-soluble modification of the oil-phase quantum dots comprises the following steps: dissolving solid oil-phase quantum dots in toluene, uniformly dispersing to obtain a quantum dot solution, adding thiohydracrylic acid into the quantum dot solution, carrying out oscillation reaction, standing, and drying precipitates obtained by centrifugation after precipitates are generated to obtain water-soluble quantum dot solids; and adding the aqueous-phase quantum dot solution into the bacterial liquid, and standing for reaction to finish marking. The bacteria marked by the solid oil phase quantum dots can be quickly imaged through a fluorescence microscope, and counting and morphological analysis of the bacteria can be quickly completed in combination with image analysis software capable of automatically identifying and counting the bacteria.

The invention discloses a monoclonal cell strain screening method based on fluorescence intensity, which comprises the following steps: determining a cell line, and stably transfecting fluorescent protein to cells of the cell line; a microwell plate is selected, a datum hole is set, the cells stably transfected with the fluorescent protein are separated and put into the microwell plate, a plurality of cells are put into the datum hole, and only one cell is put into the other holes; screening out single-cell pores with only one cell from the rest of pores except the reference pore; screening out clone group holes capable of growing clone groups; respectively digesting cells in the cloning group pores and then transferring the cells into new pores for culturing; screening out fluorescent screening holes; splitting proliferation holes are screened out; and determining the cells in the division proliferation hole as the screened monoclonal cell strain. According to the monoclonal cell strain screening method provided by the invention, the monoclonal cell strain which is high in fluorescence intensity and can proliferate at a normal speed can be screened, compared with fluorescence microscope observation and imaging, the method is convenient and rapid, and errors possibly generated by comparing the fluorescence intensity with human eyes are reduced.

The invention discloses an up-conversion microscopic imaging rapid spot scanning method and device based on light-operated fluorescence erasure, and the device comprises an excitation light module, a loss light module, a double-beam coupling module, a laser spot scanning module, and a fluorescence detection imaging module. When the probe is lighted by the exciting light and the scanning passes, the following loss light acts on the probe which is emitting the fluorescence, and the fluorescence loss is quickly induced, so that the residual fluorescence is extinguished (the fluorescence lifetime is shortened) and the imaging brightness is not weakened. According to the method, the trailing phenomenon caused by overlong fluorescence lifetime in the up-conversion point scanning fluorescence microscope is solved, the limitation of the fluorescence lifetime on the point scanning imaging speed is broken through, and up-conversion rapid point scanning microscopic imaging without brightness loss and trailing can be realized.

A fluorescence microscope system including an optical detection system configured to capture a raw image of a sample, the raw image including a plurality of pixels, each pixel having a brightness value and a processor, configured to determine one or more invalid pixels in the raw image, assign a predetermined value to each invalid pixel, determine a range of brightness values including the brightness values of a majority of the plurality of pixels excluding the one or more invalid pixels, and generate a processed image of the sample based on the determined range of brightness values.

The utility model discloses a multicolor excitation fluorescence microscope, and belongs to the field of microscopes. Comprising a first numerical control light source; the carrying seat is arranged below the first numerical control light source; the opening is formed in the carrying plate; the carrying plate is arranged on the carrying seat; the numerical control objective lens converter is arranged below the loading plate, and the numerical control objective lens converter corresponds to the opening; the chroscope box is connected to the interface of the numerical control objective lens converter; the dichroscope is movably arranged in the dichroscope box; the X-ray plate box is arranged at the bottom of the color mirror box; the movable sheet is arranged on the X-ray plate box in a sliding manner; the excitation optical filter is movably arranged on the moving sheet; the second numerical control light source is arranged at the bottom of the light sheet box and corresponds to the excitation light filter; and the high-definition automatic focusing camera is connected with the interface of the chroscope box. The beneficial effect of the utility model is that the multicolor excitation fluorescence microscope is provided.

The embodiment of the invention belongs to the technical field of image processing, and relates to a denoising method and related equipment applied to a fluorescence microscope image, and the method comprises the steps: obtaining a to-be-denoised microscope image; performing normalization processing on the microscope image to obtain a normalized image; a trained iKUNet-RCAN model is called, and the iKUNet-RCAN model comprises a residual error iKU-Net network and a residual error iKRCAN network; performing feature extraction on the normalized image according to the residual iKU-Net network to obtain intermediate feature data and spatial feature data; performing feature refinement on the intermediate feature data according to the residual iKRCAN network to obtain refined feature data; performing feature fusion on the spatial feature data and the refined feature data to obtain fused feature data; and carrying out image restoration on the fused feature data to obtain a denoised microscope image. According to the method, the denoising effect, the detail recovery capability and the reconstruction robustness of the fluorescence microscope image are remarkably improved.

The application relates to the technical field of microscopes, and discloses an adjustable fluorescence microscope which comprises a base, a mirror arm arranged on the top of the base, and a sample platform arranged on one side of the mirror arm; the top surface of the base is provided with a lifting device; and one end of the sample platform is movably connected with the lifting device. The fluorescence microscope controls the sample platform to move downwards through the lifting device; a first filter automatically turns over around a fixed shaft to the outside of the sample platform under the action of gravity, thereby automatically providing a horizontal operation space for the sample platform; after sample replacement is completed, the sample platform only needs to be lifted to a preset observation position; when the sample is replaced, the sample platform only needs to be controlled to move, manual operation of the filter is not required, so that the sample can be replaced by the staff in a high-frequency sample replacement scene, the human time cost for sample detection is shortened, and the overall working efficiency and operation convenience of the microscopic observation system are improved.

The application relates to an ANA and dsDNA fluorescence image processing method, device and computer equipment. The method comprises the following steps: acquiring a fluorescence microscope shooting image; in response to a detection item selection operation on the fluorescence microscope shooting image, determining a detection item identifier associated with the fluorescence microscope shooting image; according to the detection item identifier associated with the fluorescence microscope shooting image, distributing the fluorescence microscope shooting image to a corresponding image detection pipeline, so that the image detection pipeline detects and processes the fluorescence microscope shooting image according to an image detection algorithm process matched with the detection item identifier, and obtains a structured detection result corresponding to the fluorescence microscope shooting image; and displaying the structured detection result corresponding to the fluorescence microscope shooting image. The method can improve the laboratory detection efficiency of an anti-nuclear antibody and an anti-double-stranded DNA antibody fluorescence image.

The present application relates to the field of plant biotechnology, and particularly relates to a fluorescence staining method for pollen germination of Dendrobium nobile Lindl, which comprises five steps: collecting Dendrobium nobile Lindl flowers with a stigma, removing extra parts such as sepals and only keeping the stigma, trimming the two sides of the stigma into a horizontal shape, placing it into a pre-cooled fixing solution for fixation, and then dehydrating it with gradient alcohol; cutting the dehydrated stigma along the middle line of the stigma with a scalpel; placing the cut sample into a softener for treatment to soften the tissue; transferring the transparent sample into a staining solution containing fluorescent dye for staining in dark conditions; after the completion of staining, taking out the sample, fixing it on a glass slide, covering it with a cover glass, and observing the pollen germination of the Dendrobium nobile Lindl stigma under a fluorescence microscope. The method can clearly and accurately observe the pollen germination of the Dendrobium nobile Lindl stigma, and provides strong technical support for the related research of Dendrobium nobile Lindl and other plant materials with large stigmas.

The utility model provides a kind of bacterial counting device and water treatment system, it is related to the technical field of water quality microbiological detection device, sample pretreatment module includes filter assembly, and the water inlet end of filter assembly receives inlet water sample through pipeline, and the outlet end of filter assembly is connected with dyeing bin through pipeline, and pipeline intercommunication has drain pipe between filter assembly and dyeing bin, for the redundant water sample of outer discharge;CCD imaging module includes the imaging platform of pipeline intercommunication with the output end of dyeing bin, and automatic focusing assembly and fluorescence microscope are arranged on the upper side of the central axis of imaging platform, for showing the bacterial colony of fluorescent dye dyeing, and fluorescence microscope is electrically connected with CCD camera;CCD camera is electrically connected with image processing module, for outputting bacterial concentration and statistical distribution diagram, the technical problem of increasing culture time consumption and high detection cost of artificial operation in prior art, reaches fast detection, cost low and high accuracy technical effect.

A control system for automatedly determining an illumination intensity of at least one light source of a fluorescence microscope is provided. The control system is configured to automatedly determine, after a change in a light path, a control value for the illumination intensity of the at least one light source in order to achieve a desired value of an inspection parameter characterizing sample inspection. The light path comprises at least one of: an illumination path from the at least one light source to the sample and an imaging path from the sample to at least one detector. Determining the control value is based on: (i) a value of the illumination intensity that was set before the change in the light path, (ii) a value of the inspection parameter used before the change in the light path, and (iii) a physical model of the light path.

The invention discloses an intelligent electric upright six-hole fluorescence module, and belongs to the technical field of upright fluorescence microscopes. Comprising a shell, an excitation block rotating wheel assembly, a light source rotating wheel assembly, a linkage assembly, a condensation assembly and a control assembly. The excitation block rotating wheel assembly, the light source rotating wheel assembly, the linkage assembly, the light condensation assembly and the control assembly are all installed on a bottom plate of the shell. The excitation block rotating wheel assembly is connected with the light source rotating wheel assembly through the linkage assembly. The control assembly controls the linkage assembly to work so as to drive the excitation block rotating wheel assembly and the light source rotating wheel assembly to be synchronously aligned, and then the fluorescent channel of the fluorescent module is automatically changed. A position key is arranged on the control panel to trigger a signal to the driving control panel, then the excitation block rotating disc and the light source rotating disc are controlled to rotate at the same angular speed, when the excitation block rotating disc and the light source rotating disc rotate to the designated position, the light source module and the excitation block are synchronously aligned, meanwhile, an LED lamp is lightened, and then automatic, efficient and accurate conversion of a fluorescent channel is achieved.

The application discloses a single-cell immunoblotting method based on quenchable aptamer probes and relates to the technical field of single-cell protein detection. The method comprises the following steps: S1, a single-cell suspension sample is settled in a microwell array on a composite hydrogel chip; S2, the composite hydrogel chip is placed in an electrophoresis tank, then a lysis solution is slowly poured into the tank, and incubation lysis is carried out; S3, after cell lysis, an electric field is applied to both ends of the chip, and proteins enter a gel coating layer on the surface of the chip under the action of the electric field and start electrophoretic separation; S4, after gel electrophoresis is completed, the chip is placed under an ultraviolet lamp for exposure, and the gel is taken out after exposure is completed; S5, the gel is placed in an aptamer probe solution which is targeted to target proteins, and is soaked and dyed, and the fluorescence signal intensity of the target protein molecules is measured under a laser confocal fluorescence microscope. The application can improve the detection sensitivity of target proteins and has fast imaging speed.

The invention discloses a method for measuring the film-forming coverage rate and the shear strength of a low-speed heavy-load friction interface lubricating grease deposited film based on a fluorescence method, and belongs to the technical field of lubricating grease measurement. The method comprises the following steps: vertically arranging a coaxial illumination fluorescence microscope right above a ball-disc contact area formed by a steel ball and a sapphire disc; a lubricating grease sample dyed by a fluorescent agent is preset in the ball disc contact area; after the excitation light is filtered, the excitation light is horizontally emitted into a coaxial illumination fluorescence microscope, is reflected by a dichroscope, and is vertically irradiated to a lubricating grease sample in a ball disc contact area; a fluorescence signal sequentially passes through a dichroscope and a cut-off filter, is focused and amplified by a coaxial illumination fluorescence microscope, and is captured by a camera; and converting the fluorescence signal into a fluorescence image, and carrying out morphological and gray brightness analysis to obtain the distribution form and aggregation state of the lubricating grease thickening agent in the contact area and in the peripheral friction raceway of the contact area. According to the invention, in-situ, visual and quantitative determination of the film-forming coverage rate and the shear strength of the deposited film of the lubricating grease thickening agent is realized.

The utility model relates to the technical field of fluorescence immunoassay equipment, in particular to a magnetic bead bearing plate sealing imaging mechanism which comprises a mounting bottom plate, a bearing mechanism and an imaging mechanism, the bearing mechanism comprises a bearing disc arranged on the mounting bottom plate, a bearing groove formed in the bearing disc, a shaft hole formed in the center of the bearing groove, a rotating shaft arranged in the shaft hole and a rotating motor arranged at the lower end of the bearing disc, the upper end of the rotating shaft penetrates through the shaft hole and extends upwards, and the lower end of the rotating shaft is in transmission connection with the rotating motor; the imaging mechanism comprises a supporting frame arranged at the lower end of the mounting bottom plate and a fluorescence microscope arranged on the supporting frame, an observation hole is formed in the bearing groove, and the lens end of the fluorescence microscope corresponds to the observation hole. The design is simple in structure, low in cost and stable in operation.

In optical microscopy including fluorescence microscopy, a biological sample (215) can be immersed in an aqueous liquid medium (210) during observation. Due to the refractive index mismatch between the liquid medium and a conventional glass bottom container holding the sample, image resolution is reduced when viewing the sample. To improve resolution, a sample container (100) is formed with a container body (110) having a bottom (120) with an aperture (125), a fluorinated ethylene propylene copolymer (FEP) film (150) attached to the bottom (120) to cover the aperture (125) to form a cavity-facing surface (155) on the film (150), and exposed to the liquid medium (210). Since the refractive index of FEP is close to that of water, image resolution is improved. Furthermore, the cavity-facing surface (155) is activated by plasma treatment to promote adhesion of the cavity-facing surface (155) to the biological sample (215) to enable cells in the biological sample (215) to proliferate in adherent cell culture and to immobilize the biological sample (215) during observation.

To provide an art that enables highly sensitive optical detection over a whole area of a wavelength region of fluorochrome to observe, even if an optical detector is not provided that is highest in wavelength sensitivity over the whole area of the wavelength region of the fluorochrome.SOLUTION: A fluorescence microscope device comprises: a plurality of optical detectors that is different in a wavelength sensitivity characteristic; a detection wavelength changing unit that can change a detection wavelength of each optical detector; an input device that inputs fluorochrome or wavelength range serving as an observation object; a memory that stores first information on the wavelength sensitivity characteristic of each optical detector; and an observation condition optimization unit that determines and selects an optimal single optical detector or a plurality of optimal optical detectors to be used in observation of the observation object from the plurality of optical detectors on the basis of the fluorochrome or wavelength range serving as the observation object input by the input device, and the first information stored in the memory, and optimizes the detection wavelength change unit for the observation of the observation object. When using the plurality of optical detectors in the observation of the observation object, the fluorescence microscope device is configured to aggregate an intensity signal of fluorescence detected by each optical detector.SELECTED DRAWING: Figure 1

This disclosure generally relates to methods and compositions for detecting one or more target nucleic acid sequences (e.g., nucleic acid barcodes) in situ in biological samples. Fluorescence microscopy is one of the most important, widely used, and powerful imaging techniques in biomedical research. The spatial resolution of modern fluorescence microscopy has improved to the point where sub-diffraction-limit resolution is routinely possible. For various spatial biological applications, it is often desirable to visualize specific nucleic acid sequences in situ.

The invention relates to a fluorescence microscopy system comprising a nonlinear optical medium (ML) and comprising: - a laser source (SL) suitable for delivering first laser pulses (IL1) having a first wavelength λ1; - a power optical modulator (MP) suitable for modulating a power of the first laser pulses (IL1) so as to form second laser pulses (IL2) having different optical powers; - said optical medium (MO) being suitable for the second pulses to propagate by undergoing self-focusing within the optical medium so as to define, for each second pulse, a focal point having a longitudinal position in the optical medium that is different from the other second pulses, laser radiation originating from the optical medium and having the second self-focused pulses, referred to as third pulses, being named the output beam (FS); - a microscope (M) suitable for: - collecting a first fluorescence radiation generated via the first wavelength λ1, by illuminating a sample (Ech) with the output beam; then - acquiring, from the first collected fluorescence radiation, at least one image of the sample for each of the third pulses illuminating the sample, each at least one image being associated with a transverse observation plane of the sample that is different from the other images.

This invention discloses an RPA-T7-CRISPR-based absolute quantification chip for ctDNA. The system employs a microarray digital partitioning structure as the reaction carrier, integrating an RPA isothermal amplification system, a T7 transcription amplification system, and a CRISPR / Cas13a specific recognition system within multiple independent reaction units. After the sample enters the chip, sample allocation, target nucleic acid amplification, transcription amplification, fluorescent reporter molecule cleavage, and signal reading are completed in a closed chip flow path. Then, based on fluorescence microscopy observation, the number of positive reaction units is counted, and their percentage of the absolute copy number of ctDNA in the sample is calculated. This chip, with its digital microarray partitioning and closed flow path design, integrates nucleic acid isothermal amplification, transcription amplification, specific recognition, and fluorescence readout, achieving rapid and highly sensitive absolute quantification of low-abundance ctDNA.

The sample was treated with the formula Ln[BD] n wherein Ln is a member of the lanthanide series of elements, BD is a β-diketone, and n is an integer, to stain one or more bacterial spores in the sample; and detecting a fluorescent signal from the one or more stained bacterial spores in the sample using a fluorescence microscope.

Method for determining cell viability in a cell culture of biological cells for the biotechnological production of proteins, in particular antibodies, characterized in that a fluorescent dye selected from 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonate (Bis-ANS) or 8-anilinonaphthalene-1-sulfonate (ANS) is used as a detection agent, and wherein the fluorescent dye is present in the cell culture at a concentration of 0.1 µM to 10 µM, the fluorescent dye selectively penetrates cells with damaged cell membranes and binds to intracellular proteins, the labeled cells are detected by excitation in the UV range and detection of the fluorescence in the green wavelength range, and the determination of cell viability is carried out using an automated imaging fluorescence microscope.

The application provides a binder, comprising modified polybutadiene and / or modified polystyrene butadiene copolymer, a main chain of the modified polybutadiene comprising butadiene repeating units and modified butadiene repeating units; a main chain of the modified polystyrene butadiene copolymer comprising styrene repeating units, butadiene repeating units and modified butadiene repeating units, the modified butadiene repeating units being at least one functional side group grafted to a main chain of butadiene, the functional side group having a fluorescent effect. The application also provides a preparation method of the binder, a negative electrode sheet, a secondary battery and an electric device. The binder provided by the application can directly characterize, in situ, the distribution of the negative electrode binder in the negative electrode sheet by fluorescence microscopy, whether the negative electrode binder has the phenomenon of floating and the like, and then analyze the failure of the secondary battery.

A fluorescence microscopy metrology system includes an optical system configured to generate first light and second light having different wavelengths, a microscope body configured to irradiate a sample, coated with a fluorescent material, with the first light and the second light received from the optical system, and to receive fluorescence reflected from the sample, an image detection device configured to detect a fluorescence image corresponding to the received fluorescence, and a nanostructure analysis device configured to measure line edge roughness (LER) from the detected fluorescence image, to analyze power spectral density (PSD), or to detect a nanoparticle defect.