318 results about "Fluorescence microscope" patented technology

The invention discloses an oral risk assessment method and system based on image recognition and a medium, and relates to the technical field of image recognition, and the method comprises the steps: carrying out the block segmentation of a user oral image, activating a lesion recognizer to recognize the features of an image block, carrying out the matching of an oral disease, and obtaining a corresponding flora type set; imaging the target flora by using a fluorescence microscope, and extracting fluorescence characteristics; and generating an oral risk decision of the target user in combination with the identified disease information and the fluorescence characteristics. Therefore, the technical effects of multi-dimensional image comprehensive analysis and evaluation accuracy and efficiency improvement are achieved.

The invention discloses a microscopic image integer pixel alignment method based on sub-pixel edge offset detection, and relates to the field of microscopic image processing. The problems that an existing sub-pixel registration technology depends on iterative optimization or a complex deformation model, and real-time processing is difficult; sensor sampling errors and mechanical translation errors need to be compensated through a high-resolution camera or a precise displacement table, and the system cost is high. According to the invention, through sub-pixel-level offset compensation, sub-pixel alignment can be realized without depending on a high-precision displacement table, and the hardware cost is remarkably reduced. The method comprises the following steps: extracting image edge feature points by using an edge detection algorithm, fitting a gray centroid to calculate a sub-pixel offset, and counting an average offset; generating an affine transformation matrix according to the average offset, resampling the image, and aligning pixel values to an integer coordinate grid; and finally, cutting the image edge according to the interpolation algorithm type to eliminate the ghost. The method is also suitable for the application fields of fluorescence microscope multi-view splicing, super-resolution reconstruction and the like.

A deep learning-based digital / virtual staining method and system enables the creation of digitally / virtually-stained microscopic images from label or stain-free samples. In one embodiment, the method of generates digitally / virtually-stained microscope images of label-free or unstained samples using fluorescence lifetime (FLIM) image(s) of the sample(s) using a fluorescence microscope. In another embodiment, a digital / virtual autofocusing method is provided that uses machine learning to generate a microscope image with improved focus using a trained, deep neural network. In another embodiment, a trained deep neural network generates digitally / virtually stained microscopic images of a label-free or unstained sample obtained with a microscope having multiple different stains. The multiple stains in the output image or sub-regions thereof are substantially equivalent to the corresponding microscopic images or image sub-regions of the same sample that has been histochemically stained.

The present invention discloses a method and system for fusing visible light images and fluorescence images, and relates to the field of medical image processing technology. The method comprises: performing image registration on a visible light camera and a near-infrared camera to obtain registration parameters; synchronously acquiring visible light images and fluorescence images, preprocessing the received fluorescence images, and aligning the pixels of the visible light images and the preprocessed fluorescence images according to the registration parameters; performing pixel fusion on the aligned visible light images and fluorescence images to generate a multispectral image; and sequentially merging the fused multispectral images to generate a corresponding video sequence. The present invention adopts offline camera registration, and utilizes the registration parameters to efficiently and accurately align visible light images and fluorescence images. By enhancing the preprocessing, a high-quality fluorescence image can be generated. The fusion weight is calculated based on the edge intensity value and the fluorescence intensity value, so that the weighted fused multispectral image can simultaneously maintain edge and fluorescence intensity information, and is suitable for medical equipment such as fluorescence microscopes / endoscopes.

The invention belongs to the field of molecular sieve material characterization, and particularly relates to a method for analyzing aluminum atom pairs in a ZSM-5 molecular sieve based on super-resolution fluorescence microscopic imaging, which comprises the following steps: activating a ZSM-5 molecular sieve sample; contacting the obtained sample with probe molecules; washing the ZSM-5 molecular sieve sample, and removing unbound or nonspecifically bound probe molecules; imaging the marked sample by using a super-resolution fluorescence microscope to obtain an image sequence containing a single fluorescent molecule emission event; processing the image sequence, determining a space coordinate of each fluorescence event, and reconstructing a super-resolution fluorescence image exceeding an optical diffraction limit; and based on the space coordinates of the fluorescent dots in the super-resolution fluorescence image, carrying out statistics to obtain the position, surface density or distribution information of the aluminum atom pairs in the ZSM-5 molecular sieve. According to the method disclosed by the invention, nanoscale spatial resolution, direct imaging and quantitative analysis of Al-pair in ZSM-5 (Zeolite Socony Mobil-5) can be realized.

2.1. To increase the resolution of quasi-confocal line scanning microscopes with a main beam splitter for coupling the illumination and detection beam paths, the "re-scan" method involves descanning the sample light in the detection beam path by the illuminating beam deflection unit. After descanning, the sample light is scanned again in the detection beam path by moving it across the sensor behind the confocal aperture by a second beam deflection unit in a direction transverse to the linear focus. To increase resolution in the longitudinal direction, the illumination line can be structured. This optical arrangement has the disadvantage of significant light losses due to the large number of optical interfaces that must be passed through. 2.2. A microscope in which the illuminating beam deflection unit is optically positioned between the light source and the main beam splitter, allowing the sample light to reach the sensor away from the illuminating beam deflection unit (non-descanned), is more light-sensitive and flexible. A control unit, in one operating mode, moves the sample light beam across the sensor using only the second beam deflection unit. Depending on the operating mode, synchronization with the first beam deflection unit may be provided. 2.3. Due to its high light sensitivity, the microscope is particularly suitable for fluorescence microscopy.

Devices, systems, and methods are provided for in vivo fluorescence imaging. Disclosed herein is an implantable miniature fluorescence imager on a chip having a custom imaging array with angle selective gratings, fiber optics, or microcollimators for image deblurring, and optical filters that can be tuned to image fluorescence from multiple fluorophores simultaneously. Power is supplied by an on-chip power source or transmitted to the chip from an external transducer such as an ultrasound transducer, electromagnetic transducer, inductive transducer, or radiofrequency transducer. Wireless communication may be provided by electromagnetic or ultrasound links to the device. The function of a fluorescence microscope is provided in a millimeter-scale device that can be readily implanted in tissue and used to image fluorescently labeled cells in vivo. The small size of the fluorescence imager makes possible sustained in vivo imaging with real-time monitoring of multiple cell types within Shifting the dynamic diseased tissue or a tumor.

The application belongs to the technical field of biology and specifically relates to a method for analyzing glycans on single extracellular vesicles in a fluid sample. The method uses magnetic nanoparticles to capture and manipulate EVs, based on the specific affinity of lectins for specific glycan structures, uses polydisperse microdroplets to separate the captured EVs, realizes local signal amplification in the droplets through enzymatic reaction, adopts fluorescence microscopy to collect images of the droplets, and thus realizes identification and analysis of the glycan components on single EVs. The preparation process of the microdroplets formed by the oscillating emulsification method of the application makes the entire detection process more simple and fast, does not require any microfluidic chip and special device, and can be completed in a centrifugal tube, and all the microdroplets are generated in parallel and quickly, and the amount of microdroplets can be flexibly expanded. Moreover, compared with the traditional ELISA, the method has high sensitivity, low detection limit and small sample consumption.

The invention discloses an online optical clear solution for three-dimensional structure imaging of a large-volume biological sample, which is characterized in that the solution comprises iohexol, urea, 1, 2-propylene glycol, dimethyl sulfoxide, 3-[3-(cholamidopropyl) dimethylamino] propanesulfonic acid inner salt and water. The invention further discloses a three-dimensional imaging method for the large-volume biological sample. The method comprises the following steps: carrying out transparentizing treatment on the biological sample by using the online optical transparentizing solution, and carrying out three-dimensional imaging on the transparentized biological sample by using a fluorescence microscope. The fluorescence microscope comprises a confocal microscope and / or a light sheet microscope. When the online optical transparent solution is used for imaging, the transparent depth of a surface sample is large, the single imaging depth can be increased, and the cutting thickness can be improved, so that the cutting frequency is reduced, and finally the data acquisition efficiency of a large-volume sample is improved.

The invention discloses a ctEVs separation and analysis method with time resolution capability and application thereof. The ctEVs separation and analysis method comprises the following steps: S1, culturing cells by adopting glycometabolism markers; or injecting the animal by adopting a glycometabolism marker; step S2, manufacturing a fishbone-shaped micro-fluidic chip modified by streptavidin; s3, separating and analyzing the new tumor source extracellular vesicles by the micro-fluidic chip, and co-incubating the extracellular vesicles and the aptamer probe co-modified by Biotin and DBCO to realize biotinylation of the new tumor source extracellular vesicles; the aptamer probe is a heterologous multivalent aptamer probe; step S4, capturing biotin-labeled target extracellular vesicles by adopting a chip; and S5, acquiring a chip image by using a fluorescence microscope, and analyzing the concentration of the target extracellular vesicles in the sample according to a fluorescence signal. The method can be used for screening tumor circadian rhythm markers and the like.

The invention discloses a serum migration body identification method which comprises the following steps: extracting a serum migration body from serum by performing multiple centrifugal treatment on the serum; carrying out immunofluorescence staining treatment on the serum migration body to obtain an immunofluorescence stained serum migration body; and carrying out imaging treatment on the serum migration body subjected to immunofluorescence staining by utilizing a super-resolution fluorescence microscope SIM to identify the serum migration body. Whether an extracellular vesicle in serum is a migration body or not is identified from the two aspects of biochemistry and shape, so that the defect that the serum migration body is identified only from the shape in the prior art is overcome.

The invention discloses a method for evaluating improvement of skin inflammation and aging based on a zebra fish model and application thereof. The method comprises the following steps: S1, establishing a blank group; s2, establishing a model group; s3, establishing a sample group; s4, performing constant-temperature incubation; s5, observing the zebrafish of the blank group, the model group and the sample group under a body type fluorescence microscope, and recording the anti-inflammatory efficiency and the anti-wrinkle efficiency of the zebrafish; and S6, evaluating the skin inflammation and aging improving effect of the sample according to an anti-inflammation and anti-aging calculation formula. The method has the advantages of low cost, short test period, high reproducibility, high visualization degree, intuitive and understandable test result and the like, and can be used for accurately grading the efficacy of the cosmetic raw materials for improving skin inflammation and aging from the whole animal level.

The invention relates to the technical field of aquatic animal cell culture, in particular to a rapid enrichment and separation method for gonad somatic cells of plectropomus leopardus. Comprising the following steps: selecting healthy plectropomus leopardus for anesthesia; the method comprises the following steps: taking gonad tissues, performing sterile treatment, cutting, centrifugal washing and filtering, inoculating the gonad tissues into a culture medium for primary culture to obtain gonad cells emigrated from tissue blocks, performing trypsin-EDTA digestion and centrifugal washing, and treating the gonad cells with 1 * PBS containing polysorbate; after closing, antibody incubation and washing, the marking effect is verified through a fluorescence microscope, and specific enrichment of gonad somatic cells is achieved; and filtering and purifying the enriched fluorescence-labeled cells, placing the cells under a fluorescence microscope, accurately picking fluorescence-positive cells under a fluorescence visual field, transferring the fluorescence-positive cells into a culture dish containing a complete medium, inoculating the separated fluorescence-positive cells into the optimized complete medium, and carrying out purification culture, so that the cells grow in a wall-attached manner and stable passage is realized.

A light beam is imaged within a sample and images of the sample are generated based on light received from the sample in response to the light imaged within the sample. A wavefront modulating element modifies a wavefront of the received light and / or a wavefront of the light imaged within the sample. One or more known aberrations are introduced into at least one image of the sample and, based on at least two images of the sample, where the images include a raw image and at least one image that includes a known aberration, an aberration of a wavefront of light emitted from, and / or provided to, the sample is estimated. The wavefront modulating element is controlled to modulate the wavefront of light emitted from, and / or provided to, the sample, such that the estimated aberration of the wavefront of light emitted from, and / or provided to, the sample is reduced.

In various aspects, microscope systems are described, comprising or corresponding to optical microscopes, such as for example a transillumination microscope or a fluorescence microscope. The microscope systems may be used independently or configured with or integrated into another device or system, such as an imaging mass spectrometer or imaging mass cytometer. In various embodiments of this aspect, the microscope system is used to obtain one or more images of a sample, such as at a variety of different light collection wavelengths and / or for a variety of different illumination conditions. The microscope systems can employ various optical components and configurations allowing for high throughput imaging, such as components for imaging the sample at suitable resolutions with a wide field of view and / or for spatially separating different detection channel wavelengths from light output (e.g., transmitted or emitted light) from the sample.

The invention discloses a light field microscopic self-supervised denoising system and method fusing a physical process. The system comprises a light field imaging subsystem and a self-supervised denoising subsystem. The light field imaging subsystem is used for capturing multi-view light field original data of a sample; the self-supervised denoising subsystem is used for processing original data of a light field, preprocessing the original data of the light field in a training stage, and decomposing the original data into multi-view sub-images; the method comprises the following steps: constructing original data of a training set through two different self-supervision strategies, and then generating a self-supervision training data pair through RL deconvolution according to a physical optical process; and finally, a denoising model is obtained through 3D neural network training. In the reasoning stage, light field original data to be reconstructed are preprocessed and then subjected to RL deconvolution to generate input data to be denoised, the input data are input into the trained denoising model, and a reconstruction result is output. According to the invention, the problems of noise amplification, data dependence and poor universality of the light field fluorescence microscope are effectively overcome.

To provide an information processing apparatus capable of more easily reducing fluorescence crosstalk of an image acquired by a fluorescence microscope.SOLUTION: The information processing apparatus 10 according to the present disclosure includes a control unit 11 for acquiring a learning model constructed by learning a third image according to a fourth image on the basis of learning data in which the third image is related to the fourth image including fluorescence crosstalk, the third image not including a fluorescence crosstalk acquired by a fluorescence microscope 1, and for generating a second image in which fluorescence crosstalk of a first image of a sample (S) having been acquired by a fluorescence microscope (1), is reduced based on the acquired learning model.SELECTED DRAWING: Figure 8

The invention discloses a rapid bacterium detection and counting method based on thiohydracrylic acid quantum dot fluorescence labeling, and the labeling method comprises the following steps: carrying out water-soluble modification on oil-phase quantum dots to obtain water-soluble quantum dot solids, uniformly dispersing the water-soluble quantum dot solids in water, and adjusting the pH value to 12-13 to obtain a water-phase quantum dot solution, the method for water-soluble modification of the oil-phase quantum dots comprises the following steps: dissolving solid oil-phase quantum dots in toluene, uniformly dispersing to obtain a quantum dot solution, adding thiohydracrylic acid into the quantum dot solution, carrying out oscillation reaction, standing, and drying precipitates obtained by centrifugation after precipitates are generated to obtain water-soluble quantum dot solids; and adding the aqueous-phase quantum dot solution into the bacterial liquid, and standing for reaction to finish marking. The bacteria marked by the solid oil phase quantum dots can be quickly imaged through a fluorescence microscope, and counting and morphological analysis of the bacteria can be quickly completed in combination with image analysis software capable of automatically identifying and counting the bacteria.

The invention discloses a monoclonal cell strain screening method based on fluorescence intensity, which comprises the following steps: determining a cell line, and stably transfecting fluorescent protein to cells of the cell line; a microwell plate is selected, a datum hole is set, the cells stably transfected with the fluorescent protein are separated and put into the microwell plate, a plurality of cells are put into the datum hole, and only one cell is put into the other holes; screening out single-cell pores with only one cell from the rest of pores except the reference pore; screening out clone group holes capable of growing clone groups; respectively digesting cells in the cloning group pores and then transferring the cells into new pores for culturing; screening out fluorescent screening holes; splitting proliferation holes are screened out; and determining the cells in the division proliferation hole as the screened monoclonal cell strain. According to the monoclonal cell strain screening method provided by the invention, the monoclonal cell strain which is high in fluorescence intensity and can proliferate at a normal speed can be screened, compared with fluorescence microscope observation and imaging, the method is convenient and rapid, and errors possibly generated by comparing the fluorescence intensity with human eyes are reduced.

1. The name of this design product: Multi-channel LED microscope light source module. 2. Purpose of this design product: used as a light source for fluorescence microscope illumination. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The invention relates to an aqueous-phase bio-friendly wide-spectrum up-conversion luminescent mixed-phase CdSe: Yb quantum dot and a preparation method thereof, and belongs to the field of nano luminescent materials. Comprising the following steps: preparing a Se precursor, preparing a Cd + Yb precursor, injecting a Se precursor solution into a Cd + Yb precursor solution according to the molar ratio of Se to Cd + Yb of 1: 3-1: 5, heating from room temperature to 80-90 DEG C in 5-10 minutes, heating from 80-90 DEG C to 100-110 DEG C in 20-30 minutes, continuously heating at the temperature of 100-110 DEG C, condensing, refluxing and reacting for 2-4 hours, and finally generating cubic phase and hexagonal phase coexisting CdSe: Yb quantum dots. The CdSe: Yb quantum dot is low in toxicity and biological friendly, promotes reproduction of macrophages swallowing the quantum dot, and is suitable for tracing cells under weak-intensity multiple full-wave band exciting light matched with a biological fluorescence microscope.

The invention discloses an up-conversion microscopic imaging rapid spot scanning method and device based on light-operated fluorescence erasure, and the device comprises an excitation light module, a loss light module, a double-beam coupling module, a laser spot scanning module, and a fluorescence detection imaging module. When the probe is lighted by the exciting light and the scanning passes, the following loss light acts on the probe which is emitting the fluorescence, and the fluorescence loss is quickly induced, so that the residual fluorescence is extinguished (the fluorescence lifetime is shortened) and the imaging brightness is not weakened. According to the method, the trailing phenomenon caused by overlong fluorescence lifetime in the up-conversion point scanning fluorescence microscope is solved, the limitation of the fluorescence lifetime on the point scanning imaging speed is broken through, and up-conversion rapid point scanning microscopic imaging without brightness loss and trailing can be realized.

A fluorescence microscope system including an optical detection system configured to capture a raw image of a sample, the raw image including a plurality of pixels, each pixel having a brightness value and a processor, configured to determine one or more invalid pixels in the raw image, assign a predetermined value to each invalid pixel, determine a range of brightness values including the brightness values of a majority of the plurality of pixels excluding the one or more invalid pixels, and generate a processed image of the sample based on the determined range of brightness values.

The utility model discloses a multicolor excitation fluorescence microscope, and belongs to the field of microscopes. Comprising a first numerical control light source; the carrying seat is arranged below the first numerical control light source; the opening is formed in the carrying plate; the carrying plate is arranged on the carrying seat; the numerical control objective lens converter is arranged below the loading plate, and the numerical control objective lens converter corresponds to the opening; the chroscope box is connected to the interface of the numerical control objective lens converter; the dichroscope is movably arranged in the dichroscope box; the X-ray plate box is arranged at the bottom of the color mirror box; the movable sheet is arranged on the X-ray plate box in a sliding manner; the excitation optical filter is movably arranged on the moving sheet; the second numerical control light source is arranged at the bottom of the light sheet box and corresponds to the excitation light filter; and the high-definition automatic focusing camera is connected with the interface of the chroscope box. The beneficial effect of the utility model is that the multicolor excitation fluorescence microscope is provided.

The embodiment of the invention belongs to the technical field of image processing, and relates to a denoising method and related equipment applied to a fluorescence microscope image, and the method comprises the steps: obtaining a to-be-denoised microscope image; performing normalization processing on the microscope image to obtain a normalized image; a trained iKUNet-RCAN model is called, and the iKUNet-RCAN model comprises a residual error iKU-Net network and a residual error iKRCAN network; performing feature extraction on the normalized image according to the residual iKU-Net network to obtain intermediate feature data and spatial feature data; performing feature refinement on the intermediate feature data according to the residual iKRCAN network to obtain refined feature data; performing feature fusion on the spatial feature data and the refined feature data to obtain fused feature data; and carrying out image restoration on the fused feature data to obtain a denoised microscope image. According to the method, the denoising effect, the detail recovery capability and the reconstruction robustness of the fluorescence microscope image are remarkably improved.

An L-form ribonucleic acid (L-RNA) aptamer-antisense oligonucleotide (ASO) conjugate comprise the L-RNA aptamer, which comprises a ribonucleic acid sequence. The ASO comprises a deoxyribonucleic acid sequence selected from a group. A method of imaging amyloid precursor protein (APP) rG4 in a cell comprising transfecting the cell with a messenger RNA of APP, permeating the cell, contacting the permeated cell with a cyanine3 (Cy3) labeled APP nucleic acid probe and the L-RNA aptamer-ASO conjugate, and subjecting the product to fluorescence microscopy analysis to produce an image of the APP rG4 in the cell.

The invention discloses a method for evaluating activity of a single CTC through deep learning based on nucleus morphology. The method comprises the following steps: acquiring cell nucleus images of CTCs in different vitality states through a fluorescence inverted microscope, constructing five state data sets of the cell nucleus by utilizing Cellpose3.0 and ImageJ, realizing accurate positioning and classification by combining an improved YOLOv8x model, and calculating a single cell vitality value by adopting transfer learning and confidence coefficient weighting. The method has the advantages of no label, no toxicity and single-cell-level resolution, solves the problems of group signal dependence, phototoxicity interference and low sensitivity of a traditional method, and provides a new tool for tumor metastasis monitoring and individualized treatment.

The application relates to the technical field of microscopes, and discloses an adjustable fluorescence microscope which comprises a base, a mirror arm arranged on the top of the base, and a sample platform arranged on one side of the mirror arm; the top surface of the base is provided with a lifting device; and one end of the sample platform is movably connected with the lifting device. The fluorescence microscope controls the sample platform to move downwards through the lifting device; a first filter automatically turns over around a fixed shaft to the outside of the sample platform under the action of gravity, thereby automatically providing a horizontal operation space for the sample platform; after sample replacement is completed, the sample platform only needs to be lifted to a preset observation position; when the sample is replaced, the sample platform only needs to be controlled to move, manual operation of the filter is not required, so that the sample can be replaced by the staff in a high-frequency sample replacement scene, the human time cost for sample detection is shortened, and the overall working efficiency and operation convenience of the microscopic observation system are improved.

The application relates to an ANA and dsDNA fluorescence image processing method, device and computer equipment. The method comprises the following steps: acquiring a fluorescence microscope shooting image; in response to a detection item selection operation on the fluorescence microscope shooting image, determining a detection item identifier associated with the fluorescence microscope shooting image; according to the detection item identifier associated with the fluorescence microscope shooting image, distributing the fluorescence microscope shooting image to a corresponding image detection pipeline, so that the image detection pipeline detects and processes the fluorescence microscope shooting image according to an image detection algorithm process matched with the detection item identifier, and obtains a structured detection result corresponding to the fluorescence microscope shooting image; and displaying the structured detection result corresponding to the fluorescence microscope shooting image. The method can improve the laboratory detection efficiency of an anti-nuclear antibody and an anti-double-stranded DNA antibody fluorescence image.

The present application relates to the field of plant biotechnology, and particularly relates to a fluorescence staining method for pollen germination of Dendrobium nobile Lindl, which comprises five steps: collecting Dendrobium nobile Lindl flowers with a stigma, removing extra parts such as sepals and only keeping the stigma, trimming the two sides of the stigma into a horizontal shape, placing it into a pre-cooled fixing solution for fixation, and then dehydrating it with gradient alcohol; cutting the dehydrated stigma along the middle line of the stigma with a scalpel; placing the cut sample into a softener for treatment to soften the tissue; transferring the transparent sample into a staining solution containing fluorescent dye for staining in dark conditions; after the completion of staining, taking out the sample, fixing it on a glass slide, covering it with a cover glass, and observing the pollen germination of the Dendrobium nobile Lindl stigma under a fluorescence microscope. The method can clearly and accurately observe the pollen germination of the Dendrobium nobile Lindl stigma, and provides strong technical support for the related research of Dendrobium nobile Lindl and other plant materials with large stigmas.