119 results about "Sample contamination" patented technology

The invention is directed to methods for detecting and quantifying nucleic acid contamination in a tissue sample of an individual containing T cells and/or B cells, which is used for generating a sequence-based clonotype profile. In one aspect, the invention is implemented by measuring the presence and/or level of an endogenous or exogenous nucleic acid tag by which nucleic acid from an intended individual can be distinguished from that of unintended individuals. Endogenous tags include genetic identity markers, such as short tandem repeats, rare clonotypes or the like, and exogenous tags include sequence tags employed to determine clonotype sequences from sequence reads.

A biochip and a method for separating a target substance contained in a sample on the biochip are disclosed. The biochip comprises a substrate, a sample loading portion disposed on the substrate, a channel in fluid communication with the sample loading portion, and carbon nanotubes arrayed in intervals in the channel. The sample is loaded on the sample loading portion and flowed through the channel. The target substance is selectively separated from the sample according to the interval sizes between the carbon nanotubes. According to the present invention, various substances in a sample may be easily separated or filtered by flowing through the channel having the carbon nanotubes, and thus, sample contamination and/or experimental error are reduced.

In some embodiments, an apparatus and a system, as well as a method and an article, may operate to obtain a formation fluid sample from a formation adjacent to a wellbore disposed in a reservoir, determine the sample saturation pressure of the formation fluid sample, repeat obtaining the formation fluid sample and determining the sample saturation pressure over a selected time period or number of samples, and determine a predicted ultimate formation fluid saturation pressure based on multiple determinations of the sample saturation pressure. The sample saturation pressures measured over selected time periods can be used to determine fluid sample contamination. Additional apparatus, systems, and methods are disclosed.

Aiming at the lack of an integrated micro-fluidic chip capable of realizing capture, culture and batch differential administration of single cells simultaneously in the prior, the invention provides an integrated micro-fluidic chip and system for capture, culture and administration of single cells and belongs to application of micro-fluidic chip technologies to the field of cell pharmacology analysis. The integrated micro-fluidic chip comprises a cell capture and culture unit, a drug diffusion unit and a drug supply unit, which are sealed in sequence from top to bottom; the system employing the integrated micro-fluidic chip comprises the integrated micro-fluidic chip, a fluorescence microscope, image processing equipment and a digital injection pump. A preparation method of the integrated micro-fluidic chip comprises the following steps: photo-etching baseplate plane figures on the silicon plates of the three units to etch grooves, pouring a liquid chip material into the grooves, and obtaining the three units after the liquid chip material is solidified; conducting sealing bonding and punching to obtain the integrated micro-fluidic chip. The integrated micro-fluidic chip provided by the invention integrates the three functions of capture, culture and batch differential administration of single cells and effectively avoids sample contamination.

The invention relates to a pretreatment method of Acesulfame-K, comprising the following steps: firstly conducting nitric acid digestion treatment, then conducting high-temperature ashing treatment, and finally dissolving. The pretreatment method disclosed herein is an improvement based on orginal dry ashing and wet ashing, simplified the operation process, and effectively reduces the possibility of sample contamination. The invention further provides a detection method for detecting potassium in Acesulfame-K after pretreating the Acesulfame-K with the above pretreatment method, characterized by pretreating an Acesulfame-K sample with the above pretreatment method and then conducting instrument detection of potassium, thus the precision of the detection can be raised, and the content of potassium in the original sample can be accurately reflected.

The invention discloses a non-invasive biomass online detection device used for shake-flask culture. The device comprises a master control unit, a shake flask capable of containing biological turbid liquid and a fixing sleeve covering the outer side of the shake flask. A detection probe is connected to the fixing sleeve on the outer side of the bottom of the shake flask. The detection probe comprises a substrate capable of being attached to the bottom of the shake flask, a light source, a photoelectric sensor and an acceleration sensor, wherein the light source, the photoelectric sensor and the acceleration sensor are arranged on the substrate. The light source can generate a light path obliquely irradiated into the shake flask. The photoelectric sensor is located below the light path and used for measuring scattering light generated after the light path is irradiated to the biological turbid liquid. The light source, the photoelectric sensor and the acceleration sensor are all connected with the master control unit. According to the non-invasive biomass online detection device, a turbidity measurement method is adopted for performing online detection on the concentration of bacterial liquid cultured in the shake flask through a back scattering light measurement technology, and the concentration of the biological turbid liquid in the shake flask can be detected under the condition that culture equipment is not broken. The non-invasive biomass online detection device is simple in structure, low in cost, and capable of effectively improving detection efficiency and precision and avoiding sample contamination.

The invention belongs to the technical field of radar signal processing, and discloses a method for sparse reconstruction of a conformal array clutter covariance matrix. The method comprises the following steps: setting a conformal array as a uniform semicircular array composed of N array elements; determining the position and the array element pattern of each array element, determining the beam steering vector of the conformal array, and getting the array pattern of the conformal array; selecting an observation range gate l of clutter data, getting space-time steering vectors respectively corresponding to Nc clutters on the lth observation range gate and the clutter power corresponding to the lth observation range gate; and getting clutter data of a to-be-detected unit. Based on the prior knowledge of carrier aircraft information and array information and the data of the to-be-detected unit, a clutter plus noise covariance matrix is sparsely reconstructed using the method disclosed by the invention. The method avoids the problems of insufficient samples and sample contamination.

The invention relates to the technical field of particle analysis and discloses a particle shape analyzing device based on a laser holography imaging method and a working mechanism of the particle shape analyzing device. The device comprises a laser, a microscope lens, a diaphragm, a transparent observation piece and an image sensor, wherein a fluid inlet and outlet hole channel is formed in the transparent observation piece. The laser holography imaging method is adopted to directly perform interference on particles in a to-be-measured fluid, holographic imaging information is produced and then is subjected to data inversion processing, space imaging information reflecting the particle shape can be obtained, so that a filter membrane is not needed, sample contamination can be avoided, measurement errors and consumable costs can be reduced, and the operating process of the device is simplified. Besides, the device has the advantages of high real-time performance, high particle resolution and wide detection object range, and actual application and popularization are facilitated.

This invention relates to supercritical fluid extraction devices and the HPLC. Specifically it is a supercritical fluid extraction-liquid chromatography system, including supercritical fluid extraction devices, the first three-way valve, the second three-way valve, trapping column, HPLC pump, analysis column and detector. Supercritical fluid extraction device connects with an interface of the first three-way valve, and the other two interfaces of the first three-way valve connect with the inlet of trapping column and liquid chromatography pump. Trap-export terminal connects with one interface of the second three-way valve, the other two import terminal interfaces of second three-way valve connect with the analytical column inlet and the associated waste water bottles, analytical column export terminal links to detector. This invention uses supercritical fluid extraction device to achieve sample active ingredient extraction, on-line analysis, avoiding the sample contamination and deterioration.

A sample analyzer and a sample collection and distribution method thereof are provided. A sample collection and distribution module conducts a first collection on a sample to be tested; and then the collected sample is assigned to a plurality of measurement modules according to a predetermined sequence. Compared to a way of multiple sample collection and multiple sample distribution, the method provided by the invention simplifies the steps of sample collection and distribution, improves the test efficiency of liquid samples, and avoids the risk of sample contamination caused by a plurality of sample collections.

The invention relates to a universal method for controllably preparing nano particles on a graphene sheet. The method comprises the steps of selecting a graphite oxide colloidal aqueous solution and a high-purity Ag target, suspending the Ag target in the graphite oxide colloidal solution, ceaselessly mixing the colloidal aqueous solution with a magnetic mixer, irradiating the target with a laser, and regulating the density and dimensions of the Ag nano particles by changing irradiation time and laser power. The method overcomes the defects that the application of graphene or graphite oxide is limited due to nonuniform distribution of the nano particles prepared by a chemical method on the sheet, additives are required to result in sample contamination, instruments are expensive, the preparation cost is high, and large-scale preparation cannot be achieved when a physical method is used for preparing a graphene based composite. The invention provides a liquid laser ablation method, The graphene based composite can be prepared no matter functional groups are available on the graphene sheet, a technology is simple, the synthetic quantity is large, and the method is green and environment-friendly and can be popularized and used in the industrial field.

The invention provides a device, system and method for sample collection wherein a sample can be separated into multiple portions of sample at the site of collection. The separation of portions of sample occurs by separating parts of the specimen collection device. Each of the separable parts contacting a portion of sample can be deposited into separate vessels. The device reduces the risk of sample contamination, because the separable parts can be separated by the action of a release mechanism that allows a user to separate the parts without touching or otherwise contacting the sample collecting region. The release mechanism may be trigger-activated to expel a separable part of the device.

The invention discloses a light path precise adjusting and converting device in an ultra-high vacuum test cavity to evaluate a multi-cavity reflector set through the same light source, and belongs to the technical field of optics under the ultra-high vacuum environment. The device comprises an EVU light source, a sample contamination cavity, a gate valve, a light path precise adjusting and converting device, a reflector set vacuum cavity and an exposure cavity. The path precise adjusting and converting device adopted in the ultra-high vacuum environment can accurately adjust and position the spatial position of a reflector by means of the three-point supporting, two-dimension moving and two-dimension rotating and precise-adjustable method, it is ensured that the axis of a reflector rotating shaft system must be concentric and coplanar with the reflecting face of the reflector, an elastic element is arranged on the vertical plane of the rotating shaft system based on the isosceles triangle stability principle, it is ensured that a tension spring hanging nail is located on the extension line of rotating shaft center line, it is ensured that a tension spring can be in the same deformation and stress state before and after converting, and then the reflector can be positioned. The converting device can be operated outside a vacuum tank, and the reflector can be fast switched, positioned and focused.

The invention provides a piston type medical centrifuge tube. The piston type medical centrifuge tube comprises a centrifuge tube body and a piston arranged in the centrifuge tube body, wherein the bottom of the centrifuge tube is provided with a nipple, one end of the nipple is connected with the centrifuge tube, and the other end of the nipple is detachably connected with a nipple cover and/or a conversion port; an external column is arranged above the piston and detachably connected with a long handle; the piston type medical centrifuge tube can also comprise an aseptic packaging bag used for storing all parts subjected to bactericidal treatment. The invention further provides a preparation method of the platelet rich plasma which is prepared by means of the piston type medical centrifuge tube. The piston type medical centrifuge tube can improve convenience of making PRP, sample contamination which occurs in the operation process can be completely avoided, the platelet collection rate can be further increased, doping of impurities such as red blood cells and white blood cells is reduced, and different kinds of PRP can be collected according to different usage modes.

The invention discloses a stool sampler, comprising a plastic container, a sampling rod, a connecting piece, a liquid containing tube and a dropping nozzle cover. The plastic container and the connecting piece are in screw connection; the liquid containing tube is sleeved outside the connecting piece and the plastic container; the connecting piece comprises an external thread part, a large diameter part, a small diameter part and a broken rod part; the external thread part is connected with screw at the port of the plastic container; the large diameter part cooperates with a tube nozzle section of the liquid containing tube; the outer circumference of the large diameter part is provided with an annular buckle, which cooperates and clamps with an annular groove arranged on the inner wall of the tube nozzle section of the liquid containing tube; the small diameter part and a necking part of the liquid containing tube fit closely and are in sealing connection; the broken rod part is arranged in a liquid containing chamber of the liquid containing tube; a central hole of the connecting piece runs through the external thread part, the large diameter part, the small diameter part and until the broken rod part; and an axial rib end of the sampling rod is inserted into the central hole of the connecting piece. The technical proposal has advantages of simple structure, low manufacturing cost, convenient usage, reduced sample contamination and convenience for retention and removal of the sample solution.

The invention relates to the field of a bar code scanning technology, and discloses an automatic rotary bar code scanning structure. The automatic rotary bar code scanning structure comprises a rotation disc frame, multiple test tube clamps, a scanning head for scanning bar codes on test tubes, and a driving mechanism for driving the test tube clamps to rotate. The test tube clamps are movably connected with the rotation disc frame, the test tube clamps are provided with driving ends and clamping ends for clamping and fixing the test tubes, and the driving ends of the test tube clamps are connected with the driving mechanism; and the scanning head is arranged at the outer side of the rotation disc frame. The scanning head is arranged at the outer side of the rotation disc frame for scanning the bar codes on the test tubes, so that manual hand-operated scanning is unnecessary, the scanning efficiency is high, and the labor cost is low; and the test tubes are fixed in the clamping ends of the test tube clamps, the driving ends of the test tube clamps are driven through the driving mechanism to enable the test tube clamps to rotate, and the bar codes on the test tubes right face scanning laser emitted by the scanning head, so that manual hand-operated adjustment of the positions of the test tubes is unnecessary, risks of scanning errors are avoided, and the phenomenon of sample contamination in the test tubes, caused by manual contact, is avoided.

The invention discloses a spittle collecting and detecting device, which comprises a reagent strip, a support plate, a spittle collector and a spittle collector support, wherein the reagent strip is provided with a reaction section and a display section, the support plate is used for holding the reagent strip, the spittle collector is used for collecting spittle, the spittle collector support is used for mounting the spittle collector and hinged to the support plate, and the spittle collector can contact with the reaction section of the reagent strip. The spittle of a testee is collected by the spittle collector and can be detected by the reagent strip, the method is convenient and rapid without bringing any discomfort to the testee, and restrictions are avoided. Besides, the reagent strip and the spittle collector are placed on the support plate and the spittle collector support respectively, so that sample contamination is avoided, and the accuracy rate of detection is effectively increased.

The invention is directed to methods for detecting and quantifying nucleic acid contamination in a tissue sample of an individual containing T cells and/or B cells, which is used for generating a sequence-based clonotype profile. In one aspect, the invention is implemented by measuring the presence and/or level of an endogenous or exogenous nucleic acid tag by which nucleic acid from an intended individual can be distinguished from that of unintended individuals. Endogenous tags include genetic identity markers, such as short tandem repeats, rare clonotypes or the like, and exogenous tags include sequence tags employed to determine clonotype sequences from sequence reads.

The invention provides a measurement method for migration of hydrophobicity of the surface of a polyolefin insulator. The method comprises the following steps of sample pretreatment, contamination solution preparation, sample contamination, contamination degree check and characterization of migration of hydrophobicity. A certain area of the surface of a polyolefin insulator sample is cleaned withabsolute ethyl alcohol, and then flushed with deionized water; powdered sodium chloride serves as a salt component for preparation of the contamination solution, powdered diatomite or kaolin serve asan ash component, and ethyl alcohol or methyl alcohol or propyl alcohol or a mixture thereof serves as a dispersing agent; the contamination solution directly contaminates the surface of the sample through brush application by a clean banister brush or with a spraying method; by comparing the weight difference of the sample before and after contamination, the contamination weight per unit area iscalculated to check the contamination grade; 96 hours after migration, a static water contact angle of the sample is measured, and a spray method is used for determining the hydrophobicity level. Themeasurement method is also applicable to the measurement of migration of hydrophobicity of various insulators such as porcelain, glass, silicone rubber and epoxy, and has the advantages of simple steps, simplified operation, short drying time of contaminated samples and the like.

The invention discloses a genomic DNA extraction kit comprising a solution A, NaAC, isopropanol, 75% ethanol and ddH2O containing 50 [mu]g/mL RNA enzyme. The solution A consists of Tris-HCl, EDTA, SDS, NaCl and N-sodium lauroyl sarcosine. The invention also discloses a method for extracting the genomic DNA by the kit. The kit and method provided by the invention are suitable for rapid extraction of general type genomic DNA from plants, fungi and bacterial samples, have no need of liquid nitrogen grinding, can effectively avoid sample contamination, have the advantages of low cost, short time,and no need of organic solvent extraction, and reduce the experimental dangerousness.