185 results about "DNA extraction" patented technology

The invention discloses a method for evaluating enhancement and release seedling resources based on environmental DNA polymerization analysis. The method comprises the following steps: carrying out gridding partition on a target water area, collecting a water sample through a designed sampling scheme, and carrying out DNA extraction and high-throughput sequencing to obtain species sequence information of each sampling point. Sequencing data is subjected to species identification by using a bioinformatics method, released species are identified, a spatial abundance model is established, and a preliminary distribution map is generated. And establishing a DNA degradation kinetic model in combination with water area environmental parameters, and carrying out reverse correction on abundance distribution. And through a resource inversion model coupled with hydrodynamics, analyzing biomass distribution characteristics and migration laws of the release group, and obtaining a resource evaluation result. And finally, a species environment preference model is constructed based on migration path analysis, an optimal release area is matched in a target water area, a scientific scheme including release point locations, opportunities and quantity is generated, and a whole-process technical support and a decision basis are provided for enhancement and release.

The invention relates to the technical field of crop molecular breeding and detection, in particular to a sweet waxy corn molecular detection system based on an sh1 gene InDel marker and application of the sweet waxy corn molecular detection system, the sweet waxy corn molecular detection system is composed of a forward primer, a reverse primer and a transposon specific primer, and the sequences are shown as SEQ ID NO: 1-3. The sh1 gene InDel marker-based sweet waxy corn molecular detection system provided by the invention can be used for corn sh1 gene Mu insertion rapid detection and / or corn molecular marker-assisted sweet waxy corn breeding. By adopting a sweet waxy corn molecular detection system based on the sh1 gene InDel marker, the accuracy rate is 98.7%. And the detection cost of each sample is 0.15 dollars, and compared with the traditional SSR marking technology, the cost is reduced to 3% of that of the traditional SSR marking technology. From DNA extraction to final result interpretation, the whole detection process takes 3 hours.

The invention discloses a mountainous area surface matrix physical property prediction method, and relates to the technical field of geological exploration, and the method comprises the steps: obtaining mountainous area surface point cloud data, separating vegetation from ground points through a point cloud classification algorithm, and generating a digital elevation model and a digital surface model; based on the digital elevation model and the digital surface model, a quantum gravity gradiometer is adopted to move along a contour line for measurement, a gravity gradient tensor is obtained, terrain interference signals are eliminated, and a vertical density profile is obtained through inversion; identifying a density abnormal region according to the vertical density profile, acquiring an undisturbed soil column sample by adopting a drilling unit, determining a grain composition and moisture characteristic curve, performing DNA extraction and microorganism sequencing on the undisturbed soil column sample, and calculating the relative abundance of the nitro spirillum phylum. According to the method, the dynamic coupling model is constructed through fusion of quantum gravity gradient measurement and microbiological omics data, and the problem of insufficient vertical density-permeability coefficient coupling modeling precision of a traditional method is solved.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention relates to the technical field of biology, and particularly discloses a gene Panel sequencing method for intrahepatic cholestasis in a gestation period, which comprises the following steps: collecting saliva, performing DNA extraction and purification on a sample, breaking the purified sample, treating the broken sample to obtain a supernatant A, performing linker connection and purification on the supernatant A to obtain a supernatant B, and sequencing the supernatant B to obtain the gene Panel of intrahepatic cholestasis in a gestation period. The invention relates to a Panel detection technology for detecting 186 cholestasis genes covering bile acid metabolism, which comprises the following steps: preparing a supernatant B, carrying out pre-PCR and purification treatment on the supernatant B to obtain a pre-PCR product, carrying out liquid phase hybridization and capture, configuring a PCR reaction system, carrying out Post-PCR reaction and purification to obtain a supernatant C, quantifying the supernatant C, screening ICP susceptible genes through mixed sample sequencing, collecting DNA (Deoxyribonucleic Acid) through a saliva sampling mode, and detecting the 186 cholestasis genes covering bile acid metabolism. Other hereditary intrahepatic cholestasis diseases can be excluded, and ICP genetics susceptibility genes are defined.

A sampling device including an intake pump coupled with a volume controller to provide a known volume of samples such as liquid collected through a specialized hollow membrane filter. Once the known volume of sample is filtered the intake pump is turned off, an air pump turns on and removes residual or excess sample. The membrane filter is then preserved for extraction of DNA / RNA.

The present application relates to a deoxyribonuclease mutant and its application in preparing PDRN. The amino acid sequence of the deoxyribonuclease mutant is shown as SEQ ID NO. 1, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 2. The present application also provides the application of the deoxyribonuclease mutant and / or the encoding gene of the deoxyribonuclease mutant in preparing PDRN, and the specific application method. The present application is based on the deoxyribonuclease expressed by the DNASE1 gene of bovine pancreas tissue, and the amino acids at positions 69, 132 and 215 are selected for iterative saturation mutation. Compared with the wild-type enzyme, the enzyme activity of the deoxyribonuclease mutant A69H-S132R-Q215C is increased by 2.15 times, reaching 4.1x10 6 U / mL, and the DNA extraction rate of salmon PDRN prepared by using the mutant enzyme prepared by the present application is 15.2%, the DNA content is 99.1%, and the molecular weight is 1.05 million Daltons, which is significantly improved compared with the wild-type enzyme, and the PDRN in this molecular weight range has more significant biological activity.

The invention discloses a mitochondrial molecular marker for identifying acrossocheilus fasciatus from different water systems and application of the mitochondrial molecular marker. A primer kit of the acrossocheilus fasciatus mitochondrial molecular marker comprises a primer pair as shown in SEQ NO: 1 and SEQ NO: 2, and / or a primer pair as shown in SEQ NO: 3 and SEQ NO: 4; the acrossocheilus fasciatus mitochondrial molecular marker is formed by amplifying primers contained in the acrossocheilus fasciatus mitochondrial molecular marker primer kit. The identification method comprises the following steps: carrying out DNA extraction on acrossocheilus fasciatus to be identified; carrying out PCR (Polymerase Chain Reaction) amplification on the extracted DNA target fragment by using the primer pair; sequencing the PCR amplification product to obtain a base sequence, comparing the base sequence with the acrossocheilus fasciatus mitochondrial molecular marker, and identifying the water system source of the acrossocheilus fasciatus to be identified according to the specific site of the acrossocheilus fasciatus. According to the method, acrossocheilus fasciatus geographical populations distributed in different water systems are distinguished from gene features, and technical guarantee is provided for acrossocheilus fasciatus proliferation and releasing parent sources and offspring seed traceability.

1. Name of the product in this design: Reagent Kit. 2. Purpose of this design: Packaging box for packaging DNA extraction and testing reagents. 3. The key design elements of this product are: pattern, shape, and their combination. 4. The image or photograph that best illustrates the key design points: Design 1 3D view. 5. Design 1 is designated as the basic design.

The invention relates to a water environment MST primer performance evaluation method, device and equipment and a medium. The method comprises the following steps: acquiring a water environment sample; the water environment sample comprises a target pollution sample and a non-target microorganism sample; performing DNA extraction on the water environment sample, and performing concentration standardization treatment on the water environment sample to obtain a positive sample library and a negative sample library; respectively carrying out amplification reaction on a target primer and the positive sample library and the negative sample library to obtain amplification curve data and melting curve data; based on the melting curve data and the amplification curve data, obtaining a sensitivity index and a specificity index corresponding to the target primer; obtaining a primer performance result based on the sensitivity index, the specificity index and a circulation threshold value in the amplification curve data; the primer performance result is used for representing whether the primer performance reaches the standard or not. By adopting the method, the accuracy of primer performance evaluation can be improved, and the reliability of water environment MST detection is guaranteed.

The application discloses a kind of fluorescence colorimetric double-mode detection streptococcus agalactiae without aerosol pollution method, comprising the following steps: (1) strain culture and DNA extraction;(2) the combination of UDG and LAMP;(3) UDG-LAMP-CRISPR fluorescence mode detection system;(4) colorimetric mode detection system: read image RGB value in G value, G value significantly increases, and it is positive result.This patent innovatively combines UDG, LAMP, CRISPR and G4, introduces connection probe to realize "fluorescence-colorimetric" single system double-mode detection.The two detection modes of the detection platform can be freely split or combined, and can meet the needs of different detection environments.By combining UDG system and LAMP technology, aerosol pollution possibly carried in amplification process is avoided.Connection probe is introduced, CRISPR fluorescence detection is combined with G4 colorimetric detection, a detection system with "fluorescence-colorimetric" two detection modes is established, which is used for quantitative identification and cross verification of streptococcus agalactiae.

Portable Capsule-Based DNA Extraction Device

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.

The invention belongs to the technical field of biology, and particularly relates to an extracting solution for extracting DNA of sorghum seeds, a kit and an extracting method and application of the extracting solution. In order to solve the technical problems that the existing sorghum DNA extraction method is complicated, the extraction efficiency is low and long-term preservation cannot be realized, the invention provides a sorghum seed DNA extracting solution which comprises a DNA quick extracting solution and a cleaning solution, the rapid extracting solution contains Tris-HCl, NaCl, Tween-20 and EDTA (Ethylene Diamine Tetraacetic Acid); and the cleaning solution contains Tris-HCl and Tween-20 (polysorbate-20). By combining the extracting solution or the prepared kit with cellulose filter paper treated by NaCl, the DNA of the sorghum seeds can be rapidly extracted within 20 seconds, the extraction quality is high, the DNA of the sorghum seeds can be preserved for a short time, and meanwhile, the method has no inhibition effect on downstream PCR (Polymerase Chain Reaction). Therefore, the extracting solution, the kit and the extracting method can be quickly applied to fields or detection sites.

This invention relates to DNA extraction methods from soil and sediments with high humic substances content. The method focuses on the soil preparation phase; removing salts and cations with two different Tris-EDTA (TE) buffers, followed by precipitating humic substances with aluminum hydroxide solution and washing again with TE and cell lysis phase; urea is used as denaturing agents for the cell lysis phase and protein degradation phase while preventing the rediffusion of humic substances with CTAB. The purification can be performed following both the classic phenol chloroform method and using commercial DNA purification columns. This method is very accessible with operation time varying from 1h40 min to 2h30 min. The invention involves the use of TE and aluminum hydroxide solutions for soil preparation, and urea and CTAB as denaturing agents during cell lysis, in the specified concentrations, order, and incubation time, to prevent contamination of final DNA with humic substances

The invention discloses a method for counting the number of wild rock sheep based on a microsatellite molecular marker. The method comprises the following steps: preprocessing a rock sheep manure sample and extracting DNA (Deoxyribonucleic Acid); performing double PCR amplification on the microsatellite marker, wherein each group of microsatellite primers comprises two pairs of specific primers; carrying out electrophoresis separation on a PCR product by using 12% polyacrylamide gel, and reading the size of an allele fragment of each microsatellite site through GeneMapper software after silver staining and developing; analyzing the acquired allele data by using software, and counting the number of different wild rock sheep individuals in the region according to an individual identification standard; the individual identification standard is as follows: if the genotypes of all 10 microsatellite loci are completely the same, or only one allele of one locus is different, the same individual is determined. The method is particularly suitable for non-invasive individual identification, genetic relationship identification and population quantity statistics of plateau wild rock sheep populations, and provides technical support for rock sheep resource protection and dynamic monitoring.

The invention provides a full-process automatic all-in-one machine for DNA extraction, amplification and sequencing. The full-process automatic all-in-one machine comprises a cabin body, the cabin body is internally provided with a first space, and the first space is internally provided with a biological sample extraction workbench for realizing the whole process of DNA extraction, amplification and sequencing; the top of the cabin is provided with an air inlet duct, the side surface or / and the bottom of the cabin is provided with an air outlet duct, and the air inlet duct and the air outlet duct form negative pressure in the cabin; an amplification area is arranged on the biological sample extraction workbench, a cooling base used for reducing pollutants generated in the amplification process is arranged in the amplification area, and an air suction plate is arranged on the side face of the amplification area; the air suction plate is communicated with the air outlet channel, and pollutants generated in the amplification area are discharged out of the cabin body through the air outlet channel. Pollutants generated in the cabin body can be effectively prevented from facing three positions outside from gaps or cabin door lamps of the cabin body, so that pollution to the surrounding environment is avoided, and the safety of biological experiments is further improved. In addition, the integrated cabin is provided, and the requirement for the operation space of the biological experiment is effectively reduced.

The application discloses a plant genome DNA extraction reagent, which comprises Buffer 1 lysate, Buffer 2 precipitate and Buffer 3 combination liquid; the Buffer 1 lysate comprises sodium chloride, PVP40, sodium metabisulfite and SDS; wherein the concentration of the sodium chloride is 250-1200 mM; the mass percentage of the PVP40 is 0.5%-3%; the concentration of the sodium metabisulfite is 50-150 mM; and the mass percentage of the SDS is 0.7%-2.5%; the Buffer 2 precipitate comprises acetate; wherein the concentration of the acetate is 2.2-4.3 M; and the Buffer 3 combination liquid comprises guanidine hydrochloride; wherein the concentration of the guanidine hydrochloride is 0.5-2.5 M. The extraction reagent does not contain organic solvents such as phenol and chloroform, and can improve the removal rate of polyphenols.

The invention discloses a method for detecting active watermelon bacterial fruit blotch bacteria by a PMA-PCR (Polymerase Chain Reaction) technology, which comprises the following steps: (1) collection and suspension of bacteria in a sample to be detected: suspending the sample with normal saline to obtain a bacterial suspension; (2) PMA treatment: adding propyl azide bromide (PMA) into the bacterial suspension; (3) blue light crosslinking: irradiating the bacterial suspension treated by the PMA under blue light to activate the covalent binding of the PMA and dead bacteria DNA (Deoxyribose Nucleic Acid); (4) DNA extraction: centrifugally collecting thalli, and extracting DNA by adopting a kit method; (5) PCR amplification: carrying out amplification by using a specific primer pair; and (6) result judgment: detecting an amplification product through agarose gel electrophoresis, wherein a target strip appears in an active bacterium sample, and no strip exists in a dead bacterium sample. The invention establishes a rapid, convenient, accurate and efficient method for detecting the active watermelon bacterial fruit blotch, so as to improve the disease detection capability and management efficiency.

The invention relates to a device for automatically extracting, purifying and amplifying (PCR) regions of Mycobacterium tuberculosis DNA and recording results in real time. The device consists of a single-use cartridge, an ultrasonic generator, an emitter, and an analyzer in the form of a device capable of interacting with the single-use cartridge and managing the processes of DNA extraction and subsequent PCR and detecting a fluorescent signal in real time inside the enclosed space of the cartridge. The device further comprises a filter element for keeping a pathogen in a ventilation well of a cell of the single-use cartridge into which a sample to be analyzed is placed, as well as at least one source of UV light arranged inside and providing for the disinfection of the inner cavities of the device. The technical result is that of protecting an inner cavity between a lid and the surrounding environment against contamination of the analyzer and of the surrounding space by a pathogenic agent.

The invention relates to the technical field of agricultural detection and plant disease and insect pest recognition, in particular to a rapid identification method for infected sweet potato small weevils, which is technically characterized by comprising the following steps: collecting suspected infected sweet potato samples and hermetically enriching volatile organic gases; acquiring a response signal containing a plurality of gas sensitive elements through electronic nose equipment; performing normalization processing on the original response value, and extracting the maximum response value of each sensor to construct a sample odor feature vector; carrying out dimensionality reduction on the multi-dimensional response data by utilizing principal component analysis to obtain a sample principal component vector; and performing similarity comparison on the main component template and a preset main component template of the ipomoea batatas infection in a database, calculating a similarity index S by adopting cosine similarity, and judging that the infection is positive when S is greater than a set threshold value. The identification process does not need DNA extraction, does not need microdissection, is high in response speed, is suitable for field on-site rapid deployment, and has the advantages of being simple and convenient to operate, sensitive in detection, good in repeatability and the like.

The invention discloses a method for rapidly identifying in-vivo parasitic powdery plant pathogens, and relates to the technical field of molecular identification of plant pathogenic fungi, and the method comprises the following steps: 1, collecting samples such as plant leaves or stems with in-vivo parasitic pathogens such as powdery mildew and rust disease; 2, establishing a PCR amplification system; 3, adding an amplification template (powder pathogen) and carrying out PCR amplification; 4, separating, purifying and sequencing PCR products or sequencing molecular cloning transformants; and 5, carrying out nucleic acid database BLAST search on the sequencing sequence, and rapidly identifying the classification of living parasitic pathogen species causing plant diseases. The method can effectively avoid the technical bottleneck and cost expenditure that living parasitic fungi cannot be subjected to in-vitro culture to obtain pure culture thalli or an expensive trace DNA extraction kit is used for extracting genomes, and has important reference significance and practical value for rapid identification of living parasitic pathogenic bacteria capable of generating powder, such as powdery mildew and rust germs.

The application provides a DNA extraction method and application thereof. The DNA extraction method comprises the following steps: crushing cells of a sample to obtain a first mixture; mixing the first mixture with a hexammine cobalt (III) chloride solution and an adsorption carrier, performing first incubation, and obtaining a first incubation product; washing and dissolving the first incubation product to obtain DNA of the sample. The method can solve the problems of the prior art, such as complexity of the DNA extraction method and low quality of the obtained DNA, and is suitable for the field of DNA extraction.

The application discloses a kind of based on toxigenic and odor-producing gene toxigenic and odor-producing cyanobacteria field rapid monitoring system and method, a kind of based on toxigenic and odor-producing gene toxigenic and odor-producing cyanobacteria field rapid monitoring system, including water sample algal automatic enrichment module, algal cell rapid lysis and DNA extraction tube, isothermal / normal temperature PCR module, palm fluorescence detector, qualitative and quantitative algorithm and toxigenic and odor-producing cyanobacteria risk warning grade calculation software, mobile phone APP software, each module is sequentially linked.The application realizes the integrated detection of toxigenic and odor-producing cyanobacteria quickly, accurately and conveniently, solves the problem that existing technology cannot accurately distinguish between toxigenic and non-toxigenic cyanobacteria and is difficult to scientifically assess the health risk of water body, while overcoming the limitations of traditional monitoring technology, such as laboratory detection, large equipment, complex operation, long detection period, etc., and meeting the timeliness, convenience and ease of operation requirements of field scenes such as grassroots monitoring, outdoor inspection and emergency disposal of sudden water bloom.

This invention relates to the field of ecological conservation technology, specifically to a method and system for screening species that preserve ecological memory from discarded polypropylene masks. The method includes: obtaining baseline and transfer samples of biofilm from discarded polypropylene masks; obtaining a relative abundance matrix after DNA extraction, high-throughput sequencing, and quality control standardization; and extracting the top-ranking microorganisms from the transfer group to construct an independent variable feature matrix. Ecological memory retention is calculated based on the Bray-Curtis distance between the transfer group and the homologous baseline group. A random forest model is trained using the feature matrix, and key microorganisms are screened based on the error changes after shuffling the abundance. Ecological memory-preserving species are then screened based on the Spearman correlation between their abundance and retention. Finally, the intersection of ecological memory-preserving species, network key species, and neutral community key species is defined as the super-core species. This invention improves the accuracy of screening ecological memory-preserving species.

The application discloses an enhanced red blood cell lysis solution, a red blood cell DNA extraction kit and a method. The application provides an enhanced red blood cell lysis solution, which can improve the yield of red blood cell DNA. The application also provides a red blood cell DNA extraction kit and discloses a method for separating or extracting DNA from red blood cells. In addition, the application also discloses applications of the kit and the method for separating or extracting DNA from red blood cells, and the applications include applications in detecting cancer or predicting the risk of suffering from cancer. The enhanced red blood cell lysis solution and the kit provided by the application have important scientific value and application prospect.

The invention belongs to the technical field of molecular traceability of plant pathogenic bacteria, and discloses a molecular traceability method of potato stripe bacteria, which comprises eight specific SNP (Single Nucleotide Polymorphism) marker combinations located in the 80226-81462 interval of a CaLso genome, a nested PCR (Polymerase Chain Reaction) primer group for detecting the marker combinations, and a molecular traceability kit containing the primer group. The invention relates to a potato stripe pathogen molecule traceability method. The method comprises the following steps: sample DNA extraction, qPCR positive screening, nested PCR amplification, SNP site sequencing comparison and genotype analysis, so as to realize accurate determination of CaLso geographical source and haplotype. The method can be applied to CaLso quarantine and intrusion risk prevention and control of commercial crop seeds of Solanaceae or Umbelliferae imported from ports, provides technical support for monitoring pathogen propagation paths and formulating targeted prevention and control strategies, and is high in practicability.

The invention belongs to the technical field of gene inheritance, and particularly relates to a CDB simple arabian jasmine flower genetic transformation method which comprises the following specific steps: selecting different physiological parts of arabian jasmine flowers as candidate explants, performing scratch pretreatment, setting time gradient for pre-culture, and screening the optimal duration; the method comprises the following steps: screening three agrobacterium adaptive strains, namely AR1193, GV3101 and EHA105, and constructing a vector containing an RUBY visual reporter gene; according to the application, the RUBY reporter gene is adopted, the positive transformant can be directly recognized through red color development under natural light, DNA extraction and PCR detection do not need to be carried out in the early stage, the screening process is simplified, the screening period is shortened, the transformation efficiency can be improved only by lightly scratching an explant with a sterile blade, and complex tissue culture for inducing callus is not needed; the soaking method is adopted for infection, ultrasonic-assisted delivery is matched, operation is convenient and fast, professional high-end equipment is not needed, clear gradients are set for key conditions, optimized parameters can be directly reused, the test exploration cost is reduced, and the method is suitable for being popularized in different laboratories.

The invention relates to the field of DNA extraction of leather cultural relics, and discloses a method for extracting cattle genomes from vegetable tanned cattle leather cultural relics. The method comprises the following steps: after cleaning the vegetable tanned cow leather cultural relics, dehydrating, freeze-drying and grinding and crushing with liquid nitrogen; metal ions are chelated through an EDTA-Tris detanning reagent to destroy a tannin structure, so that the wrapping on DNA (Deoxyribose Nucleic Acid) is reduced; then, lysate and protease K are used for lysis and digestion, cell membranes are damaged, protein is degraded, and DNA is released; adding absolute ethyl alcohol to precipitate and separate out DNA; and finally, purifying, recovering and purifying the DNA through an adsorption column. The genome DNA extracted by the method is large in fragment, high in purity and stable and reliable in quality.

The utility model provides a device for quickly separating and storing livestock and poultry manure bacteria, which relates to the field of biological information extraction and comprises a separation tank, an upper cavity is arranged at the upper end in the separation tank, a lower cavity is arranged at the lower end in the separation tank, and an inlet is arranged on the wall of the upper cavity. According to the utility model, a plurality of links such as sample mixing, primary filtering and secondary filtering are integrated in the separating tank, so that the preparation and storage of bacterial suspension can be completed only by simple operations such as unscrewing a bottle cap, adding a solution and inverting the tank body, and by matching with a magnetic stirrer and gravity filtering; by adopting a two-stage filtering structure consisting of a built-in filter plate and a gauze net, a mixed solution containing bacteria can be rapidly separated, the purity of a suspension can be further improved, the interference of impurities on subsequent experiments such as microorganism culture and DNA extraction is effectively reduced, and the accuracy and reliability of experimental results are guaranteed.