439 results about "DNA extraction" patented technology

The invention discloses an extraction preparation method and application of algae micromolecule PDRN, and relates to the technical field of biology. The invention establishes a process for extracting PDRN from microalgae, which comprises the following steps: carrying out cell disruption on microalgae liquid, and removing cell debris to obtain filtrate; the filtrate is subjected to DNA dissolution, DNA separation and DNA purification, and a PDRN solution is obtained; wherein the dry weight of the microalgae in the algae liquid is 0.1 to 0.333 g / mL. Through process optimization, the extraction efficiency and purity of the microalgae PDRN are improved, and the extracted PDRN has excellent anti-aging and anti-inflammatory effects.

The invention discloses a method for evaluating enhancement and release seedling resources based on environmental DNA polymerization analysis. The method comprises the following steps: carrying out gridding partition on a target water area, collecting a water sample through a designed sampling scheme, and carrying out DNA extraction and high-throughput sequencing to obtain species sequence information of each sampling point. Sequencing data is subjected to species identification by using a bioinformatics method, released species are identified, a spatial abundance model is established, and a preliminary distribution map is generated. And establishing a DNA degradation kinetic model in combination with water area environmental parameters, and carrying out reverse correction on abundance distribution. And through a resource inversion model coupled with hydrodynamics, analyzing biomass distribution characteristics and migration laws of the release group, and obtaining a resource evaluation result. And finally, a species environment preference model is constructed based on migration path analysis, an optimal release area is matched in a target water area, a scientific scheme including release point locations, opportunities and quantity is generated, and a whole-process technical support and a decision basis are provided for enhancement and release.

The invention discloses a long-time inter-well injection and production connectivity discrimination method based on microorganism DNA sequencing in the technical field of oil-water well connectivity detection, which comprises the following steps of: sampling from a water injection well and a production well according to a set time interval and circulation time respectively, pretreating the sample, and then extracting DNA and sequencing DNA; based on the sequencing results of the water injection well produced fluid sample and the production well produced fluid sample, comparing and analyzing the difference of the two samples under the time scale so as to judge the injection-production connectivity; according to the method, sampling, sequencing and data analysis are carried out in a mode of screening and tracing strains and in a mode of endogenous tracing, main coordinate analysis is carried out on a sequencing result to reduce the dimension of a high-dimensional similarity matrix into a low-dimensional space, the decoupling difficulty of effective information is effectively reduced, and on the basis, the accuracy of data analysis is improved. By comparing and analyzing composition differences of microbial communities in production fluid samples of a water injection well and a production well under different time scales, the connectivity between the injection wells and the production wells is judged, and dynamic monitoring of the connectivity between the wells in a long-term and large-range mode is achieved.

The invention relates to a plant DNA rapid extraction reagent, a use method and application thereof. The reagent is prepared from sodium hydroxide, disodium ethylene diamine tetraacetate, potassium acetate, guanidine hydrochloride and Tween-80. By using the reagent, plant DNA can be rapidly extracted in a one-tube mode, the extraction rate is high, the extraction quality is good, and the molecular stability is high; the obtained sample can be directly connected with PCR or LAMP reaction, liquid nitrogen or sample freezing is not needed, and operation is easy, convenient and rapid. Compared with a traditional DNA extraction method, the method is small in reagent dosage, easy to operate and small in toxic and side effect.

The invention relates to the field of molecular biology, and discloses a fish DNA bar code database and a construction method and application thereof based on a mitochondrial genome complete sequence. The method comprises the steps of fish directory determination, fish collection, classification and identification, DNA extraction, mitochondrial whole genome sequencing and analysis, DNA bar code database building and database application. Based on the research basis of the target water area and fish historical data, the fish directory in the target water area is determined, the fish DNA bar code database is constructed by utilizing the mitochondrial whole genome sequencing technology, and accurate database support is provided for fish investigation and monitoring by applying the environment DNA technology. The problems that an existing fish public database is insufficient in integrity, miscellaneous in data source, uneven in quality and insufficient in region pertinence, possibly causes small population species comparison loss, comparison results are wrong, false positive results such as non-target region species cannot be effectively eliminated, and related species with high gene similarity are difficult to accurately identify are solved.

The invention provides a method for deducing the abundance of each family in a group based on mixed pool simplified genome sequencing, which comprises the following steps of: (1) establishing a parent 2b-rad library, and extracting SNP (Single Nucleotide Polymorphism) genotypes and sites by using a 2b-rad standard process; combining the SNP genotypes of the 2n parents into genotypes of n parent pairs according to a male parent and female parent relationship; comparing the genotypes of the parent pairs, and determining family specific tags; (2) extracting offspring individual tissues, mixing, and carrying out DNA extraction and 2b-rad library building sequencing on the mixed sample tissues; counting the sequencing depth of all the specific tag sites and calculating the secondary allele frequency of the specific tag of the mixed sample filial generation; and (3) calculating the proportion of each family in the offspring according to the sequencing depth of the specific tag site and the secondary allele frequency of the specific tag of the mixed offspring. According to the method, the overall feature distribution of the family can be accurately evaluated without sequencing each individual, so that the cost is greatly reduced.

The invention discloses a blood genome DNA extraction kit and an extraction method, and belongs to the technical field of nucleic acid extraction. The DNA extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution and an eluent, wherein the lysate is prepared from the following components: 5.5 to 6 M of guanidine hydrochloride, 0.2 to 0.3 M of NaCl, 0.01 to 0.015 M of EDTA (Ethylene Diamine Tetraacetic Acid), 0.01 to 0.015 M of glucan, Triton X-100 with the volume concentration of 1.5 to 2 percent, 0.06 to 0.1 M of dithiothreitol, Tween-20 with the volume concentration of 5 to 10 percent and 10 to 50 mM of trehalose. The components of the lysate in the kit are optimized, through the synergistic effect of the components, the cell lysis efficiency is remarkably improved, meanwhile, DNA molecules higher in purity, more complete in structure and higher in extraction yield can be obtained, and therefore the kit has good application prospects.

The invention belongs to the technical field of biomolecules, and particularly relates to an SMAD7 gene for judging chicken sex and application. Through the molecular marker and the design of a new PCR primer, the migration distance difference of two bands amplified by a ZW chromosome is 299bp, and compared with the migration distance difference of 150bp of a traditional sex identification gene CHD1 amplification system, the migration distance difference of the bands of the two items is larger, and judgment is easier; the fragments amplified by the designed PCR primer are smaller and are only 517bp and 816p, the PCR amplification time is remarkably shortened, and the sex identification time is shortened; in addition, amplification and agarose gel electrophoresis can be directly carried out by extracting chicken tissues, the sex of the chicken can be rapidly judged according to an electrophoresis result, the DNA extraction time is shortened, the time length can be controlled within 1 hour, operation is easy, the identification result is accurate, basic-level application and popularization are facilitated, and the method has a wide market application prospect.

The invention provides a rapid detection kit for bursaphelenchus xylophilus and a method and application thereof.The detection kit comprises a fluorescent RAA dry powder reagent premixed with primer probes, the primer probes for rapidly detecting the bursaphelenchus xylophilus based on RAA are designed and screened, and a primer and probe set for specifically amplifying the ITS gene of the bursaphelenchus xylophilus is obtained; no cross reaction with the bursaphelenchus mucronatus is caused; meanwhile, the kit comprises a lysis solution for rapidly extracting the DNA of the pine wood nematode suspension, and the lysis solution contains quaternary ammonium salt, isopropanol and DMSO, so that the DNA extraction quality and efficiency are remarkably improved. The bursaphelenchus xylophilus detection kit provided by the invention is strong in detection specificity, high in sensitivity, simple to operate and portable, and can rapidly detect bursaphelenchus xylophilus within 30 minutes under field conditions.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to lactation traits of buffalo and application of the SNP molecular marker, the SNP molecular marker is located at the 51st basic group of Chr1166691407, the SNP molecular marker is G or T, and the nucleotide sequence of the Chr1166691407 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the SNP molecular marker related to the lactation traits of buffalo and application of the SNP molecular marker related to the lactation traits of buffalo. The SNP molecular marker which is most related to the peak milk yield of the buffalo is obtained by means of DNA extraction and sequencing, genome comparison, whole genome association analysis and the like, and the peak milk yield of the GG type buffalo is superior to that of buffalo with other genotypes by researching the remarkable association between the genotype of the SNP molecular marker and the peak milk yield of the buffalo; the method is applied to auxiliary breeding of buffalo strains or varieties with the peak milk yield characteristic, so that the breeding period is shortened, the breeding efficiency is improved, and the buffalo produced by the bred buffalo with the peak milk yield characteristic can be greatly improved.

The invention relates to the technical field of crop molecular breeding and detection, in particular to a sweet waxy corn molecular detection system based on an sh1 gene InDel marker and application of the sweet waxy corn molecular detection system, the sweet waxy corn molecular detection system is composed of a forward primer, a reverse primer and a transposon specific primer, and the sequences are shown as SEQ ID NO: 1-3. The sh1 gene InDel marker-based sweet waxy corn molecular detection system provided by the invention can be used for corn sh1 gene Mu insertion rapid detection and / or corn molecular marker-assisted sweet waxy corn breeding. By adopting a sweet waxy corn molecular detection system based on the sh1 gene InDel marker, the accuracy rate is 98.7%. And the detection cost of each sample is 0.15 dollars, and compared with the traditional SSR marking technology, the cost is reduced to 3% of that of the traditional SSR marking technology. From DNA extraction to final result interpretation, the whole detection process takes 3 hours.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the fat content of millet grains, a KASP primer pair and application of the molecular marker. The molecular marker provided by the invention is located at the 45054761bp position of the fifth chromosome of the millet genome, and the single nucleotide polymorphism is G / A; the molecular marker is related to the grain fat content of millet, and when the genotype is AA or GA, the molecular marker has the character of high grain fat content; when the genotype is GG type, the gene has the character of low grain fat content. A KASP primer pair is developed on the basis of the molecular marker, rapid and high-throughput detection can be carried out on a sample only through DNA extraction, PCR amplification and KASP genetic typing, the millet with the high grain fat content and the millet with the low grain fat content are distinguished, the millet grain fat content character identification is practical and effective, the molecular marker can be applied to millet breeding and high-quality genetic improvement application research, and the application prospect is wide. A new choice is provided for the breeding application of improving the fat content of the millet.

The present invention discloses a microecological animal model for obstructive sleep apnea syndrome, which includes steps such as constructing a fitting model for the changing trend of OSA events, selecting target animals and performing pretreatment, preparing an animal model, obtaining fecal samples, and DNA extraction and 16S rRNA high-throughput sequencing. By constructing a fitting model for the changing trend of respiratory sleep apnea events, the present invention obtains the fitting mathematical relationship between the number of apnea and time, and establishes an animal model with SD rats. According to the obtained fitting mathematical relationship, the animal model of the experimental group is given periodic oxygen supply to establish an intestinal flora model for studying obstructive sleep apnea syndrome, which can simulate the actual physiological environment during sleep of patients. According to the changing trend of the duration of patients' respiratory sleep apnea events, a microecological animal model more consistent with the actual situation is established, making the detection result of the flora structure more accurate.

The invention discloses a chicken embryo sex determination kit directly using blood as a template and application thereof. Belongs to the technical field of biology. The kit comprises Taq DNA polymerase, an upstream primer, a downstream primer and water. Compared with the prior art, the method has the beneficial effects that a PCR reaction system is optimized by adopting the super-fidelity Taq DNA polymerase, whole blood is diluted and then is directly used as a template for PCR reaction, tedious DNA extraction is not needed, meanwhile, a PCR reaction enhancer and a PCR system stabilizer are not needed to be added into the reaction system, and the operation is simple and convenient. The method has the advantages of simple and convenient operation, less time consumption, rapid identification and the like, and avoids the defects of time and labor consumption and error proneness of a traditional method.

The invention provides a wheat gene sample automatic extraction device, which comprises an extraction processing box, supporting foot bases, a sealing door, an automatic control panel group, a vibration crushing box, a cooling sealing cover, an insulating handle, a cooling liquid nitrogen tank and an analysis detection box, the supporting foot bases are respectively in bolted connection with four corners of the lower part of the extraction processing box; the sealing door hinge is connected to the lower side of the front part of the extraction processing box; the automatic control panel set is connected to the upper side of the front portion of the extraction processing box through screws. Liquid nitrogen grinding is combined with ceramic grinding beads, and a vibration motor, a supporting tray, a locking screw, a test tube clamping seat and a constraint clamping hole are utilized, so that high-throughput treatment of multiple wheat samples can be realized at the same time, the DNA extraction cost of a single sample is reduced by 60%, and cells are uniformly broken; and the analysis detection box, the detection probe group, the cracking bin, the communicating pipe and the processor are arranged, so that different tissues such as wheat endosperm (high starch) and leaves (high fiber) can be processed.

The invention relates to the technical field of molecular biology, in particular to an extraction method of polysaccharide and polyphenol plant super-long DNA. The invention provides an extraction method of polysaccharide and polyphenol plant super-long DNA (deoxyribonucleic acid), which comprises the following steps: after extracting plant cell nucleuses, splitting by using SDS (sodium dodecyl sulfate), adding chloroform for extraction, and precipitating to obtain the super-long DNA. According to the extraction method provided by the invention, after the components of the lysate are optimized and extracted, impurities such as protein are removed in cooperation with chloroform extraction, so that the problem of low purity of DNA extracted from polysaccharide and polyphenol plants at present is solved. According to the method for extracting the super-long DNA of the polysaccharide and polyphenol plant, provided by the invention, the DNA with high purity and relatively long fragments can be obtained, the requirement of a third-generation sequencing platform on the DNA quality can be met, and good sequencing output can be obtained.

The invention belongs to the technical field of biological detection, and relates to a method for detecting lentivirus titer by a double-standard-curve RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method. Infecting the target cells by adopting a to-be-detected sample containing a recombinant lentivirus, and collecting and extracting genome DNA of the infected target cells; carrying out a viral genome RT-qPCR amplification reaction and an internal reference genome RT-qPCR amplification reaction by respectively taking the original virus standard substance and the internal reference standard substance which are diluted in a gradient manner and the extracted target cell genome DNA as templates; and calculating the lentivirus titer corresponding to the sample according to data obtained by the RT-qPCR amplification reaction. The method can be used for absolute quantitative qPCR detection of lentivirus titer, can accurately determine lentivirus successfully integrated into a cell genome, and reflects the real infection ability of the virus; in addition, the technology has high sensitivity and specificity, is not influenced by the extraction efficiency of the cell genome DNA, and ensures the reliability of the detection result.

The invention discloses application of a hawthorn leaf product in preparation of a product for improving intestinal flora and a research method, belongs to the technical field of improvement of alteration of intestinal flora, and particularly discloses application of the hawthorn leaf product in improvement of alteration of intestinal flora and the research method of the hawthorn leaf product. SPF-level male mice are selected and stored in a constant-temperature and constant-humidity illumination fixed animal room; after one week of adaptive feeding, randomly dividing the crataegus pinnatifida bunge into six groups which are respectively marked as a Control group, an HFD group, a low-dose crataegus pinnatifida bunge leaf group HL100, a high-dose crataegus pinnatifida bunge group HL500, a low-dose vitexin group Vitexin 6 and a high-dose vitexin group Vitexin 30; collecting the sample: collecting fresh mouse excrement one week before the experiment is finished; and then excrement DNA extraction and 16S rRNA sequencing are carried out to analyze intestinal flora. The invention verifies that the hawthorn leaf product can be used for preparing the product for improving the intestinal flora, and the hawthorn leaf product preferably selects hawthorn leaves or vitexin as a raw material.

The invention discloses a pretreatment method for panda excrement DNA extraction and application, and relates to the technical field of molecular biology. The pretreatment method comprises the following steps: loading newly collected excrement into a sterile sampling bag, so that a sample is quickly and completely frozen to be hard, then breaking the excrement into pieces, freeze-drying, sieving, sealing and cryopreserving. The pretreatment method can enrich DNA of a surface mucous membrane layer and trace DNA in excrement, does not need to peel off the surface mucous membrane layer, and is time-saving and labor-saving. The pretreatment operation is simple, and no reagent is needed. The pretreated excrement is small in size, good in stability, easy to store and capable of being stored in a refrigerator for a long time for standby application, and the needed storage space is small. The effective storage life and the use period of the sample can be prolonged, and technical support can be provided for molecular and genetic research of pandas.

The invention discloses a mountainous area surface matrix physical property prediction method, and relates to the technical field of geological exploration, and the method comprises the steps: obtaining mountainous area surface point cloud data, separating vegetation from ground points through a point cloud classification algorithm, and generating a digital elevation model and a digital surface model; based on the digital elevation model and the digital surface model, a quantum gravity gradiometer is adopted to move along a contour line for measurement, a gravity gradient tensor is obtained, terrain interference signals are eliminated, and a vertical density profile is obtained through inversion; identifying a density abnormal region according to the vertical density profile, acquiring an undisturbed soil column sample by adopting a drilling unit, determining a grain composition and moisture characteristic curve, performing DNA extraction and microorganism sequencing on the undisturbed soil column sample, and calculating the relative abundance of the nitro spirillum phylum. According to the method, the dynamic coupling model is constructed through fusion of quantum gravity gradient measurement and microbiological omics data, and the problem of insufficient vertical density-permeability coefficient coupling modeling precision of a traditional method is solved.

The invention discloses a method for extracting DNA from marine sediments, and belongs to the technical field of DNA extraction. The method comprises the following steps: adding sodium hexametaphosphate and a cell lysis solution into the marine sediment, uniformly mixing, incubating and centrifuging to obtain supernate; adding a chloroform-isoamyl alcohol mixed solution into the supernate, uniformly mixing, centrifuging, and removing an organic phase to obtain a water phase; and adding linear polyacrylamide and sodium chloride into the water phase, uniformly mixing, adding ethanol, precipitating in a dark place, and centrifuging to obtain the DNA extract. Compared with the prior art, the method provided by the invention has the advantages that the DNA in the marine sediment can be effectively extracted, the extraction amount is high, the concentration is high, and the obtained DNA can better meet the metagenome sequencing requirement and the subsequent PCR reaction requirement.

The invention discloses a plant genome DNA (deoxyribonucleic acid) extraction reagent. The plant genome DNA extraction reagent comprises a Buffer 1 lysis solution, a Buffer 2 precipitation solution and a Buffer 3 binding solution, the Buffer 1 lysate comprises sodium chloride, PVP40 (Polyvinyl Pyrrolidone 40), sodium metabisulfite and SDS (Sodium Dodecyl Sulfate); wherein the concentration of the sodium chloride is 250 to 1200 mM; the mass percent of the PVP40 is 0.5%-3%; the concentration of the sodium metabisulfite is 50-150mM; the mass percent of the SDS is 0.7%-2.5%; the Buffer 2 precipitation solution comprises acetate; wherein the concentration of the acetate is 2.2 to 4.3 M; the Buffer 3 binding solution comprises guanidine hydrochloride; wherein the concentration of the guanidine hydrochloride is 0.5 to 2.5 M. The extraction reagent does not contain organic solvents such as phenol and chloroform, and the removal rate of polyphenol can be improved.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention relates to the technical field of biology, and particularly discloses a gene Panel sequencing method for intrahepatic cholestasis in a gestation period, which comprises the following steps: collecting saliva, performing DNA extraction and purification on a sample, breaking the purified sample, treating the broken sample to obtain a supernatant A, performing linker connection and purification on the supernatant A to obtain a supernatant B, and sequencing the supernatant B to obtain the gene Panel of intrahepatic cholestasis in a gestation period. The invention relates to a Panel detection technology for detecting 186 cholestasis genes covering bile acid metabolism, which comprises the following steps: preparing a supernatant B, carrying out pre-PCR and purification treatment on the supernatant B to obtain a pre-PCR product, carrying out liquid phase hybridization and capture, configuring a PCR reaction system, carrying out Post-PCR reaction and purification to obtain a supernatant C, quantifying the supernatant C, screening ICP susceptible genes through mixed sample sequencing, collecting DNA (Deoxyribonucleic Acid) through a saliva sampling mode, and detecting the 186 cholestasis genes covering bile acid metabolism. Other hereditary intrahepatic cholestasis diseases can be excluded, and ICP genetics susceptibility genes are defined.

The invention provides a lysis binding solution, an extraction kit and a detection method for extracting residual DNA of host cells, the lysis binding solution comprises guanidine hydrochloride, PEG6000, tRNA, glycogen and isopropanol, the concentration of guanidine hydrochloride is 2.0-2.5 M, the concentration of PEG6000 is 1%-10% (m / v), and the concentration of isopropanol is 50-60%. According to the present invention, the host cell residual DNA extraction is performed by using the lysis binding liquid, such that the compatibility between the reagent and different sample types can be significantly enhanced, the sample pre-dilution is reduced, and the matrix interference is reduced so as to improve the extraction efficiency and the extraction accuracy of the residual DNA, and effectively overcome the limitation of the commercial kit.

According to the decalcification-free trace DNA extraction method for the old bone sample, an independent decalcification step is not adopted, a specific metal chelating agent and a phosphoramidate derivative are introduced into a bone incubation solution, the specific metal chelating agent has high chelating capacity on calcium and magnesium ions, and the specific metal chelating agent and a complex product thereof are high in water solubility and not prone to being separated out; the phosphoramidate derivative is easy to remove and does not affect the DNA adsorption efficiency of the magnetic beads and subsequent PCR amplification, the phosphoramidate derivative preferentially acts with a phosphate group fixed site on the surface of the soil, adsorption of the soil to the DNA is greatly reduced, an incubation product can be obtained to the maximum extent, then soil impurities are removed through centrifugation, and it is ensured that no adverse effect is generated on subsequent DNA adsorption of the magnetic beads. According to the method, trace DNA can be effectively extracted and purified from old bone detection materials, independent decalcification pretreatment is not needed, sample loss is avoided, and the extracted DNA can be used for forensic medicine identification.

The invention discloses a Crohn disease identification system, which comprises a data acquisition module, which is used for obtaining a fungus ITS amplicon sequence variant data set based on the results of microorganism DNA extraction and fungus ITS sequencing on respectively acquired fungus sample data of ileum end mucous membranes of healthy personnel and target patients; the model construction module is used for carrying out first training on a constructed Crohn disease recognition model by using a fungus ITS amplicon sequence variant data set based on a random forest algorithm, and determining a model characteristic strain type based on an initial Crohn disease recognition model obtained by the first training; performing second training on the initial Crohn disease recognition model according to the model characteristic strain type to obtain a target Crohn disease recognition model; and inputting the ITS amplicon sequence variant of the model characteristic strain of the individual to be identified into the Crohn disease identification model to obtain an identification result. The recognition system provided by the invention is high in recognition accuracy, good in robustness and simple and efficient in operation.

A sampling device including an intake pump coupled with a volume controller to provide a known volume of samples such as liquid collected through a specialized hollow membrane filter. Once the known volume of sample is filtered the intake pump is turned off, an air pump turns on and removes residual or excess sample. The membrane filter is then preserved for extraction of DNA / RNA.

The present application relates to a deoxyribonuclease mutant and its application in preparing PDRN. The amino acid sequence of the deoxyribonuclease mutant is shown as SEQ ID NO. 1, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 2. The present application also provides the application of the deoxyribonuclease mutant and / or the encoding gene of the deoxyribonuclease mutant in preparing PDRN, and the specific application method. The present application is based on the deoxyribonuclease expressed by the DNASE1 gene of bovine pancreas tissue, and the amino acids at positions 69, 132 and 215 are selected for iterative saturation mutation. Compared with the wild-type enzyme, the enzyme activity of the deoxyribonuclease mutant A69H-S132R-Q215C is increased by 2.15 times, reaching 4.1x10 6 U / mL, and the DNA extraction rate of salmon PDRN prepared by using the mutant enzyme prepared by the present application is 15.2%, the DNA content is 99.1%, and the molecular weight is 1.05 million Daltons, which is significantly improved compared with the wild-type enzyme, and the PDRN in this molecular weight range has more significant biological activity.

The invention discloses a mitochondrial molecular marker for identifying acrossocheilus fasciatus from different water systems and application of the mitochondrial molecular marker. A primer kit of the acrossocheilus fasciatus mitochondrial molecular marker comprises a primer pair as shown in SEQ NO: 1 and SEQ NO: 2, and / or a primer pair as shown in SEQ NO: 3 and SEQ NO: 4; the acrossocheilus fasciatus mitochondrial molecular marker is formed by amplifying primers contained in the acrossocheilus fasciatus mitochondrial molecular marker primer kit. The identification method comprises the following steps: carrying out DNA extraction on acrossocheilus fasciatus to be identified; carrying out PCR (Polymerase Chain Reaction) amplification on the extracted DNA target fragment by using the primer pair; sequencing the PCR amplification product to obtain a base sequence, comparing the base sequence with the acrossocheilus fasciatus mitochondrial molecular marker, and identifying the water system source of the acrossocheilus fasciatus to be identified according to the specific site of the acrossocheilus fasciatus. According to the method, acrossocheilus fasciatus geographical populations distributed in different water systems are distinguished from gene features, and technical guarantee is provided for acrossocheilus fasciatus proliferation and releasing parent sources and offspring seed traceability.