2552 results about "Bovine serum albumin" patented technology

The invention relates to a freeze-drying protective agent of nucleic acid amplification reaction reagents and a freeze-drying method. The invention provides a composition for freeze-drying protection, which is the following 1) or 2): 1) the composition comprises mycose, mannitol, bovine serum albumin, and water with a ratio of 4.0-25g:1-7g:1.0-7g:49-50ml; 2) the composition comprises mycose, mannitol, bovine serum albumin, Tween, Tris-HCl and water with a ratio of 4.0-25g:1-7g:1.0-7g:0.1-0.25ml:2.5*10<-3>-5.0*10<-3>mol:49-50ml. The freeze-drying protective agent of the invention is low in raw material cost, simple in operation, and suitable for large-scale production, can reduce production cost, and can effectively prolong the storage life of nucleic acid amplification reaction reagents.

The present invention belongs to the field of biological detection. Multi-line immunochromatographic test strip for semi-quantitative detection of aflatoxin B1 comprises a paperboard, wherein a water-absorbing pad, a detection pad, a gold-labeled pad and a sample pad are adhered sequentially on one surface of the paperboard from top to bottom, wherein each adjacent pads is overlapped and connected, the detection pad uses a nitrocellulose film as a backing pad, the nitrocellulose film is provided with a transverse control line, a test line I, a test line II and a test line III, wherein the control line is coated with a rabbit anti-mouse polyclonal antibody, and the test line I, test line II and test line III are coated with aflatoxin B1-bovine serum albumin conjugate (AFB1-BSA), respectively: and the gold-labeled pad is transversely coated with a nanogold-labeled anti-aflatoxin B1 monoclonal antibody. Said test strip is used for semi-quantitative detection of aflatoxin B1, and is characterized by quick detection, simple procedure and high sensitivity.

The invention discloses a method for preparing an immuno biosensor for measuring ractopamine (RAC) in the technical field of chemical detection. Nanogold, a carbon nano tube having conjugated bonding coupled RAC-bovine serum albumin, and prussian blue are modified on a glassy carbon electrode sequentially to obtain the electrochemical immuno biosensor. By the method, the electrochemical immuno biosensor which has high sensitivity, high stability and can quickly test the ractopamine can be prepared.

A precursor for the construction of chelated metal conjugates which demonstrate improved assay performance and utility in minimizing non-specific binding while maintaining specificity for target molecules is disclosed. The precursor has tridentate functionality towards multivalent ions such as iron and nickel and contains a diacetyl glycine group covalently linked via an amide to a molecule such as a proteinaceous molecule providing a primary amide group for amide bond formation. The precursor is preferably prepared in monomeric form by reacting nitrilotriacetic acid or a salt thereof in an aqueous medium at an alkaline pH of at least 8 with a proteinaceous molecule containing a primary amine group in the presence of a carbodiimide. The proteinaceous molecule may be bovine serum albumin or an enzyme such as alkaline phosphatase or horseradish peroxidase.

The invention relates to an aflatoxin B1 flow lag immunization time distinguishing fluorescence rapid-detection kit and an application thereof. The kit comprises a fluorescent test strip and a sample reaction bottle containing an europium-labeled anti-aflatoxin B1 monoclonal antibody lyophilized product, wherein the fluorescent test strip comprises a cardboard, a water absorption pad, a detection pad and a sample pad are sequentially pasted on one surface of the cardboard from top to bottom, adjacent pads are connected at the connection in an overlapping manner, the detection pad treats a cellulose nitrate membrane as a base pad, a transverse quality control line and a detection line are arranged on the cellulose nitrate membrane from top to bottom, the quality control line is coated with a rabbit anti-mouse polyclonal antibody, and the detection line is coated with an aflatoxin B1 bovine serum albumin conjugate; and the anti-aflatoxin B1 monoclonal antibody is secreted by a hybridoma cell strain having a preservation number of CCTCC NO.C201015. The kit can be used for the quantitative determination of the content of the aflatoxin B1, and has the advantages of simple operation, rapidness and high accuracy.

The invention discloses a method for preparing a chemically perforated polyvinylidene fluoride hollow fiber ultrafiltration membrane. The method comprises the following steps of: stirring and mixing polyvinylidene fluoride, a solvent, an additive and a chemical perforation agent, and preparing homogeneous membrane casting liquid; squeezing the membrane casting liquid and core liquid out from a spinning nozzle, and forming the membrane in an outer gel bath with certain components; and performing gel phase inversion and chemical post-treatment on the membrane, and thus obtaining the high-flux polyvinylidene fluoride hollow fiber ultrafiltration membrane. The method has the characteristics that: chemical enhanced perforation is performed by changing the composition of the core liquid, performing gel phase inversion and performing post-treatment according to reaction among between the core liquid, the gel bath and the post-treatment liquid and the chemical perforation agent, so that high flux, high intensity, high retention rate and high hydrophilism polyvinylidene fluoride ultrafiltration membrane can be prepared. The prepared polyvinylidene fluoride hollow fiber ultrafiltration membrane is high in performance; the flux is greater than 580 L / (m<2>h) in pure water; and the retention rate of bovine serum albumin (BSA) is over 95 percent.

The invention relates to a preparation method of a graphene-coated glass fiber composite material. The preparation method comprises the following steps of: (1) carrying out heat cleaning treatment on a glass fiber, then washing by respectively using acetone, hydrochloric acid and deionized water, and drying for later use; (2) carrying out dipping dip-coating treatment on the glass fiber in a bovine serum albumin solution to obtain a bovine serum albumin functionalized glass fiber; (3) carrying out dipping treatment on the bovine serum albumin functionalized glass fiber in a graphite oxide dispersion solution, and drying to obtain a graphite oxide-coated glass fiber; (4) reducing the obtained graphite oxide-coated glass fiber by adopting a reducing agent to obtain the graphene-coated glass fiber composite material. The preparation method disclosed by the invention is simple in process, easy to operate, and easy for realization of industrial production, and the prepared glass fiber composite material achieves the conductive grade; in addition, the prepared glass fiber composite material achieves the conductivity at 1-6 S / m, is greatly enhanced in conductivity compared with an electrical insulating glass fiber, and has great application in the fields of electromagnetic shielding and the like.

A cryopreservation solution of tissue engineering products uses DMEM culture solution as a basic solvent which is added with vitamin B, vitamin C, chondroitin sulfate, beta-integrin, cromolyn sodium, cytochalasin B, L-glutamine, bovine serum albumin, fetal bovine serum, trehalose, sodium carbonate, polysucrose-70, and dimethyl sulfoxide added during freezing storage. The cryopreservation solution provided by the invention has little toxicity to cells and long storage time, and can be stored for six months under 80 DEG C below zero, and 12 to 18 months in liquid nitrogen; and the cell viability of the resuscitated cells can be over 60%. The stored tissues can be simply and conveniently used after being resuscitated for only three to five min in water bath under the temperature of 37 DEG C and being washed by sterilized saline water. The invention can be widely used, and is suitable for tissue engineering skin, tissue engineering cornea, tissue engineering blood vessel, tissue engineering nerve and the like, and also is applicable to the cryopreservation of normal tissues.

The invention relates to a preparation method and application of an environmental estrogen electrochemical immunosensor, belonging to the technical field of electrochemical detection. The sensitivity of the sensor is obviously improved by adopting the characteristics that the conductivity of chitosan dispersed graphene is high, the stability is high, the specific surface area of a gold hybrid mesoporous silicon dioxide nano composite material is large, the biocompatibility is good, the catalytic efficiency is high and the like. The immunosensor is constructed by adopting the method of layer-by-layer self-assembly of the chitosan dispersed graphene, cross-linking agent, composite solution of nano materials and antibodies, and bovine serum albumin. In combination with specificities of antigens and antibodies, by virtue of an electrochemical workstation instrument, by recording current change before and after corresponding antigens modify the sensor, the environmental estrogens in water and food can be directly detected. The environmental estrogen electrochemical immunosensor prepared by adopting the preparation method has the advantages that the sensitivity is high, the specificity is good, the operation is easy to conduct and the detection limit is low, and the sensitive, rapid and accurate detection of various environmental estrogens in actual samples can be realized.

The invention relates to a diluent for preserving porcine semen at normal temperature. A total solute of the diluent comprises 45 to 55 percent by weight of monose combination, 15 to 25 percent by weight of amino acid combination and 25 to 35 percent by weight of electrolyte, buffer, antioxidant, stabilizing agent and antibiotics. Various substances are dissolved in double distilled water and a volume of 1, 000 milliliters is fixed so as to finally make an osmotic pressure of the diluent in the range of 330 to 360mosmo / L and make a pH value of the diluent in the range of 6.3 to 6.5. After theporcine semen is preserved at normal temperature in the environment of the diluent of the invention for 5 to 7 days, the viability can still be kept over 0.6 and the acrosom entire rate can reach 85 percent. When the porcine semen preserved by adopting the diluent of the invention is used for mating on 260 sows, the conception rate and the litter size reach 90.2 percent and 10.7 respectively and reach or exceed the levels of the import diluent. The diluent of the invention replaces the import bovine serum albumin with the monose combination and the amino acid combination, but the cost of the diluent of the invention is only 1 / 3 of that of the import diluent, so the production cost of artificial insemination of swines is reduced greatly.

The invention belongs to the field of bioinstrumentation and relates to a multi-test-line immunochromatographic test strip for semi-quantitatively detecting aflatoxin B1, comprising a paperboard, wherein a water absorbent pad, a detection pad, a gold-labeled pad and a sample pad are adhered on one surface of the paperboard from top to bottom in sequence, wherein adjacent pads are overlapped and connected at the connection part, the detection pad takes a nitrocellulose film as a base pad, the nitrocellulose film is provided with a transverse quality control line, a detection line I, a detection line II and a detection line III from top to bottom, wherein the quality control line is wrapped with a rabbit anti-mouse polyclonal antibody, and the detection line I, the detection line II and the detection line III are respectively wrapped with an aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate; and the gold-labeled pad is transversely sprayed with a nanogold-labeled aflatoxin B1 monoclonal antibody. The immunochromatographic test strip is used for semi-quantitatively detecting the aflatoxin B1 and has the characteristics of fast detection, simple operation and high sensitivity.

The invention belongs to the technical field of detection, and particularly relates to a method for detecting heavy metal ions in aqueous solution by using a fluorescent probe. The method, by providing bovine serum albumin functionalized gold nano-particles serving as the fluorescent probe and using the fluorescent probe for detecting mercury ions in a water body, obtains high sensitivity and selectivity. The method utilizes bovine serum albumin modified gold nano-particles as the fluorescent probe; during the detection, various amino and carboxyl groups on the surface of the probe can be coordinated with the heavy metal ions so as to ensure that the fluorescent probe quenches; the initial concentration of the mercury ions and the strength of fluorescence have good linear relation; and the existence of the heavy metal ions, namely mercury in the aqueous solution can be indicated through the fluorescent detection method. The method does not need large-scale instruments, can realize in-situ quick detection, has low detection limit, can be applied to more conditions and ranges, and has simple operation and low cost; and a used reagent and the operation process have no toxic or side effect.

Disclosed herein are methods for purifying immunoglobulin G (IgG). The methods feature the use of particular buffers and reagents to isolate and purify human IgG or to remove host contaminating proteins, non-human or chimeric IgG, IgG dimers, IgG aggregates, bovine serum albumin, transmissible spongiform encephalopathy, DNA, viral DNA, or viral particles from a feedstock. IgG purified by the methods described herein can be used for research, diagnostic, or therapeutic purposes.

The invention provides a frozen semen diluent formula for flocks and herds, and the formula of each 100mL of diluent comprises the following components: 1.35-1.55g of citric acid, 2.4-2.6g of trihydroxymethyl aminomethane (tris), 1.0-1.2g of glucose, 4.5-6.8ml of glycerol, 18.0-21ml of yolk, 4.5-7.5ml of bovine serum albumin (BSA), 0.45-0.75ml of vitamin B12, 50000-100000IU of benzylpenicillin potassium, 50000-100000IU of streptomycin sulfate and the balance of distilled water. The formula provided by the invention is low in cost, simple and convenient in preparation and using and operation steps, capable of effectively improving the sperm motility, the acrosome integrity rate and the plasmalemma integrity rate, and capable of reducing the sperm aberration rate.

The invention relates to a method for detection of abnormal PrP in a sample of blood or urine, said method comprising: (a) diluting the sample with buffer to comprise final concentrations of (i) 10 mM to 500 mM buffer agent; (ii) 1% to 10% w / v bovine serum albumin; and (iii) 1% to 8% w / v CHAPS; (b) adding steel particles and incubating to allow PrP binding; (c) washing the steel particles to remove diluted sample; and (d) detecting abnormal PrP captured on the steel particles using antibody capable of binding said abnormal PrP. The invention also provides compositions and kits.

The invention discloses bovine serum albumin-platinum composite nanomaterial mimetic peroxidase as well as a preparation method thereof and application. Bovine serum albumin is used as a template, and the bovine serum albumin-platinum composite nanomaterial mimetic peroxidase is prepared through biomineralization. Bovine serum albumin-platinum composite nanomaterials are prepared through the following method that chloroplatinic acid aqueous solutions are added to bovine serum albumin aqueous solutions and are mixed, sodium hydroxide aqueous solutions are added to obtain mixed solutions, and water bath heating is carried out; ultrafiltration is carried out on the solutions, then the solutions are washed, and bovine serum albumin-platinum composite nanomaterial aqueous solutions are obtained. The bovine serum albumin-platinum composite nanomaterials have excellent peroxidase activity, and can catalyze hydrogen peroxide oxidation 3, 3', 5, 5'-tetramethyl benzidine hydrochloride to be in color development. Meanwhile, the mimetic peroxidase resists acid and base, high temperature and high salinity, and has excellent short-term indoor temperature stability and long-term indoor temperature stability.

The invention belongs to technical field of crossing of nano-materials and biochemical sensing, and relates to a method for preparing a ratio fluorescence dopamine probe based on a carbon spot / copper nanocluster compound. The method comprises the following steps: firstly, preparing carbon spots emitted by blue fluorescence to perform aminophenylboronic acid modification on the surface; then preparing bovine serum albumin-stabilized copper nanoclusters emitted by red fluorescence; mixing and reacting the carbon spots with the copper nanoclusters to prepare the carbon spot / copper nanocluster compound emitted by double fluorescence; then, adding dopamine to aqueous dispersion of the carbon spot / copper nanocluster compound; using a fluorescence spectrometer for measuring a fluorescence emission spectrum; fitting a linear relationship between ratio fluorescence peak intensity and dopamine coexistence concentration; and further, constructing the ratio fluorescence dopamine probe based on the carbon spot / copper nanocluster compound. The probe is simple in preparation process, low in preparation cost, high in sensitivity and selectivity, and capable of being developed into a novel ratio fluorescence probe applied to efficient detection of dopamine.

The invention discloses the preparation method and uses of carboxymethyl chitosan and sodium alginate blend microcapsule wherein the method comprises, using water as solvent to blend the water solution of carboxymethyl chitosan and sodium alginate into blending liquid I, dissolving the contained medicament bovine serum albumin into the blended solvent I and mixing homogeneously, obtaining blended liquid II, dispersing the blended liquid II into fluid wax containing emulsifying agent, dropping CaCl2 coagulated liquid into the system, charging 10-30 mL alcohol isopropylicum and hardening to obtain micro-encapsulation, which exhibits sensitive response function to the pH changes.

The invention relates to an immunofluorescence staining method for suspension cells. The immunofluorescence staining method for the suspension cells is characterized by comprising the following steps of: collecting cells into a centrifuge tube; performing 800-gram centrifugation to collect the cells; respectively adding paraformaldehyde to fix, Triton-X100 to pass through and 1 percent bovine serum albumin (BSA) to close; adding a primary antibody and a secondary antibody sequentially to incubate; washing by an 800-gram centrifugation method after the operation of each step; performing diamino phenyl indole (DAPI) staining; and dripping on a glass slide and closing the glass slide to observe. The immunofluorescence staining method for the suspension cells has the advantages that: the immunofluorescence staining process is performed in the centrifuge tube, so the problems that the suspension cells grow on the glass slide difficultly and drop off easily in the test process are solved; the staining result is good; and the method is suitable for the suspension cells and cells which are adhered to the wall difficultly.

The invention discloses a preparation method of a lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe. The preparation method adopts quantum dots as carriers and lysozyme as a template molecule and modifies a molecular imprinting polymer layer on surfaces of the quantum dots to synthesize the novel lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe through combining selectivity of a molecular imprinting technology and fluorescent characteristics of the quantum dots. Specially, the preparation method comprises the following steps of preparing quantum dots, modifying surfaces of the quantum dots by denatured bovine serum albumin, forming a molecular imprinting layer through a polymerization reaction initiated by a selected functional monomer and cross-linking reagents, and washing away the template molecule by selected eluent. The lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe can combine accuracy and specific identification characteristics of molecular imprinting and high sensitivity characteristics of quantum dot fluorescence detection and has high selectivity and sensitivity of template molecule identification. The preparation method has simple processes, good reproducibility between different batches, good application prospects in selective identification and detection of lysozyme in an actual sample.

The invention discloses a polyvinylidene fluoride hollow fiber ultrafiltration membrane with permanent hydrophilcity and a preparation method thereof. The preparation method comprises the following steps of: sequentially adding 13 wt% to 25 wt% of polyvinylidene fluoride, 8 wt% to 16 wt% of additives and 0.02 wt% to 3.0 wt% of surface active agents and solvents in a dissolving tank, and then stirring for dissolving for 2 to 16 hours at 15 to 90 DEG C to the fully uniform state to prepare that initial membrane casting liquid; sequentially adding initiating agents accounting for 0.1 wt% to 3.5 wt% of polar monomers and the polar monomers accounting for 0.1 wt% to 5.0 wt% of the membrane casting liquid in the membrane casting liquid, and reacting for 1 to 12 hours at 15 to 90 DEG C to prepare hydrophilic membrane casting liquid; and preparing the hydrophilic hollow fiber ultrafiltration membrane by adopting the traditional dry-wet spinning technology. For the hydrophilic polyvinylidene fluoride hollow fiber ultrafiltration membrane prepared in the preparation method, the pure water permeability coefficient is greater than 230 L / m2*hr*0.1MPa, the retention rate of bovine serum albumin (67000MW) is greater than 95.0%, the breaking elongation rate of fibers is greater than 200%, and the fracture pressure is greater than 0.55 MPa. The polyvinylidene fluoride hollow fiber ultrafiltration membrane with permanent hydrophilcity is especially suitable for deep treatment and recycling of waste water, clarification and separation in the fields of biochemical engineering and medicament, and the like.

The invention discloses a kit for detecting the content of neutrophil gelatinase-associated lipocalin (NGAL). The kit comprises a reagent R1, a reagent R2 and an NGAL reagent reference standard substance, wherein the reagent R1 comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA (Bovine Serum Albumin), 0.01%-0.1% of Tween-20, 0.5%-3% of PEG (Polyethylene Glycol) and 0.1% of NaN3; the reagent R2 comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA, 0.01%-0.1% of Tween-20, 0.1% of NaN3, 1%-10% of saccharose and 0.1%-1% of NGAL antibody sensitization latex particles; the NGAL reagent reference standard substance comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA, 0.01%-0.1% of Tween-20, 0.5mM-5mM of EDTA (ethylenediamine tetraacetic acid) and 0.1% of NaN3. The kit is simple in reagent composition, high in test sensitivity, wide in linear range, high in stability, low in test cost, high in accuracy and convenient to popularize.

The invention discloses a pollution-resistant modified method for a polymer membrane containing arone perssad. The method comprises the following steps: poly(methyl)acrylic acid epoxy alkyl ester is grafted on the surface of an ultra-(micro-)filtration or nanofiltration membrane through ultraviolet graft polymerization; further reaction is conducted to generate alkyl halide; the alkyl halide is conducted by reaction with acrylic acid betaine compounds under the condition that certain catalyst, ligands or reducing agent are subsistent; the ultra-(micro-)filtration or nanofiltration membrane with favorable hydrophilcity and pollution-resistant performance is obtained. Purified water flux of the prepared ultra-(micro-)filtration or nanofiltration membrane can reach to 50-280 L / m<2>.h; the reject rate of the pollution-resistant ultra-(micro-)filtration or nanofiltration membrane to bovine serum albumin is 95-100%; the recovery rate of water flux reaches to 97-100%. The pollution-resistant modified method is characterized in that a small quantity of initiating agent functional groups are introduced quickly on the surface of the membrane through the light graft method; then graft polymerization is conducted in water phase adopting an atom free radical transferring polymerization method; the pollution-resistant modified method has the advantages that the polymerization system can be repeatedly used, and is easy in continuous production; the polymer is grafted with the zwitter ionic pair perssad with a betaine structure, so that the surface of the membrane is high in hydrophilia; the pollution-resistant capability is improved remarkably.

Provided are a kit and method for quantitatively detecting trimethylamine N-oxide. The method for quantitatively detecting trimethylamine N-oxide comprise the following steps of adding trimethylamine N-oxide in an aqueous solution of human serum albumin to prepare a series of trimethylamine N-oxide standard solutions with a concentration gradient, adding an internal standard substance and a precipitating agent to each standard solution respectively, centrifuging the obtained standard solutions, obtaining a series of supernatant, analyzing the supernatant by using liquid chromatography and mass spectrometry respectively, and obtaining the ratio of the chromatographic peak area of trimethylamine N-oxide to that of the internal standard substance in each trimethylamine N-oxide standard solution and a relationship between the ratios and concentrations of trimethylamine N-oxide; adding the internal standard substance and the precipitating agent to a to-be-tested blood serum sample, conducting centrifugation, analyzing obtained supernatant by using liquid chromatography and mass spectrometry, obtaining the ratio of the chromatographic peak area of trimethylamine N-oxide to that of the internal standard substance in the to-be-tested blood serum sample, and obtaining the concentration of trimethylamine N-oxide in the to-be-tested blood serum sample according to the relationship between the ratios and the concentrations of trimethylamine N-oxide. According to the kit and method for quantitatively detecting trimethylamine N-oxide, the problem that unreliable detection results can be caused by application of water, a bovine serum albumin solution or a phosphate buffer solution and other blank biological matrix which are far from plasma components of the human body can be avoided, and the reliability of the detection results is improved.

The invention discloses an application of a Raman encoding microsphere and a method for detecting a tumor marker by utilizing the Raman encoding microsphere, wherein the application of the Raman encoding microsphere refers to the application of the Raman encoding microsphere in tumor marker detection. The method for detecting the tumor marker by utilizing the Raman encoding microsphere comprises the following steps: dispersing the Raman encoding microsphere into a phosphoric acid buffer solution and adding a detection antibody for reaction, thus obtaining a nano probe; then utilizing bovine serum albumin to seal a space bit on the surface of the nano probe, thus obtaining a nano prober marked by Raman encoding; adding serum containing a tumor marker into a solid-phase antibody, reacting and adding the nano prober marked by Raman encoding, thus obtaining an immune complex; and after enriching the immune complex through an additionally added magnetic field, performing SERS (surface enhanced Raman scattering) spectrum detection. The Raman encoding microsphere used in the invention has an ultra-strong SERS effect, and can be used for performing quantitative analysis on a polycomponent ultratrace object, and selecting a large number of molecules with different SERS characteristic oscillation as markers to detect various matters to be detected simultaneously.

The invention belongs to the fields of molecular biotechnology and experimental methods and specifically relates to a fluorescence quantitative PCR reaction solution. The solution is characterized by comprising a PCR reaction reinforcing agent which is one or more selected from the group consisting of dimethyl sulfoxide, glycerin, bovine serum albumin, methanamide, polyethylene glycol 6000, spermidine, ammonium sulfate and betaine, e.g., dimethyl sulphoxide, bovine serum albumin and betaine. The fluorescence quantitative PCR reaction solution further comprises heatproof DNA polymerase having 5'-3' polymerase activity and 5'-3' exonuclease activity and preferably comprises heatproof DNA polymerase and hot-start heatproof DNA polymerase, both of which having 3'-5' exonuclease activity. The invention also relates to a kit containing the reaction solution and a method of applying the reaction solution in fluorescence quantitative PCR.

The invention discloses an ECL (electrochemiluminescence) immunosensor for detecting tumor markers and a preparation method and applications thereof. The immunosensor comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode is a gold electrode, cysteamine, glutaraldehyde, a tumor marker first-antibody, a tumor marker, and a composite functionalized nanosphere are sequentially decorated on the surface of the gold electrode, the composite functionalized nanosphere is simultaneously marked by a tumor marker second-antibody and an ECL marker, and non-specific active sites are closed by using bovine serum albumins. The preparation method comprises the steps of preparing the gold electrode on which the tumor marker first-antibody is fixedly carried; synthesizing the composite functionalized nanosphere simultaneously marked by the tumor marker second-antibody and the ECL marker; and finally, assembling the obtained gold electrode and the composite functionalized nanosphere into an ECL immunosensor. The ECL immunosensor disclosed by the invention is high in sensitivity, rapid in analysis speed, strong in stability and selectivity, good in reproducibility, easy to operate, and flexible in method.

The invention discloses a manufacture method of a silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and the application thereof. The manufacture method of the electrochemical immunosensor includes modifying thionine-graphene mixed solution on the surface of a glassy carbon electrode, conducting cross linking on an antibiotic antibody incubated by Ag-Fe3O4 mesoporous nanometer particles, closing non-specificity active sites by bovine serum albumin to manufacure the antibiotic electrochemical immunosensor. A detection method of antibiotic is that a reference electrode-saturated calomel electrode, an electrode-platinum filament electrode and a working electrode are correctly connected on an electrochemical working station, and immunodetection is conducted through the square wave voltammetry. The antibiotic electrochemical immunosensor has high sensitivity and selectivity, is simple in detection method and has the advantages of being quick, high in efficiency, goodin specificity, low in cost, convenient to operate and the like. It takes to 2-3 minutes to finish one detection process.