785 results about "Electrochemical detection" patented technology

The invention discloses a power distribution network collaborative management method and system based on artificial intelligence, and relates to the technical field of electrochemical detection, and the method comprises the steps: constructing a distributed edge computing node network, deploying nodes at key positions of a power distribution network, achieving the collection and preprocessing of local power data, and reducing the cross-regional data transmission pressure; an AI real-time communication scheduling model is established based on the preprocessed data, communication resources are dynamically allocated according to the operation state of the power distribution network, and fault data transmission is guaranteed preferentially; seamless interaction of multi-protocol equipment is realized through a self-adaptive protocol conversion mechanism containing protocol identification, format conversion and data verification; training a fault diagnosis model by using a federated learning framework, and enabling edge nodes to only upload parameters to a coordination center for aggregation and updating, so as to balance model precision and data privacy; when a fault is detected, a millisecond response mechanism is started, and a processing strategy is generated and executed in combination with edge local decision and central global optimization.

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.

The invention discloses a water quality heavy metal real-time detection method and system based on multi-source data fusion, and relates to the technical field of water quality detection.The water quality heavy metal real-time detection method comprises the steps that spectral data and electrochemical data of a water quality sample are obtained, feature analysis is conducted based on the spectral data, a spectral heavy metal detection model is constructed, and a spectral detection result is obtained; performing feature analysis based on the electrochemical data, and constructing an electrochemical heavy metal detection model to obtain an electrochemical detection result; and performing multi-source data fusion according to the spectrum detection result and the electrochemical detection result to obtain a water quality heavy metal detection result. By collecting spectral data and electrochemical data, the limitation of a single detection method is overcome, and the stability and reliability of the model are improved; by establishing a multi-level anomaly detection and processing mechanism and formulating corresponding processing strategies for different levels, the intelligent management of the detection process is realized, and the accuracy and credibility of the detection result are improved.

The invention discloses a liquid cooling charging module health state prediction system, and relates to the technical field of electrochemical detection, and the system comprises a multi-parameter collection unit which collects the pressure and flow data of a cooling liquid, and is provided with an independent collection node at each branch of a double-gun charging system, and provides basic data for monitoring; the dynamic calibration unit communicates with the acquisition unit, compensates data drift based on a temperature-vibration interference model of machine learning training, executes baseline calibration by using an idle period to update a reference value, and guarantees data accuracy; the leakage detection unit is used for calculating a data change rate after compensation, outputting an early warning according to a preset condition and positioning a leakage branch; and the health state evaluation unit fuses the early warning signal and the historical operation data to generate a prediction result. The problems that tiny leakage detection is difficult and a sensor is prone to interference can be solved, manual inspection is reduced, the maintenance cost is reduced, and the requirement of a high-power charging scene is met.

The invention relates to an enzyme reagent, glucose electrochemical test paper and a preparation method thereof, and belongs to the technical field of electrochemical detection. The glucose electrochemical test paper comprises an electrode substrate and an enzyme reagent arranged on the electrode substrate, the enzyme reagent is prepared from the following components in parts by mass: 5 to 7 parts of buffer solution, 0.01 to 0.2 part of hexamine ruthenium trichloride, 0.01 to 0.1 part of quinone compound, 0.1 to 1.2 parts of metal organic framework immobilized glucose dehydrogenase and 0.2 to 0.8 part of film-forming agent. According to the glucose electrochemical test paper, the detection accuracy of glucose can be improved, and the problems of large interference, small linear range, insensitive detection and the like in the prior art are improved or solved.

The invention discloses a wearable sweat glucose detection system based on multi-shell metal organic framework encapsulated enzyme and a preparation method of the wearable sweat glucose detection system. The detection system comprises a sweat collection and sensing module, an electrochemical detection module and an iontophoresis module, the sweat collecting and sensing module comprises a micro-fluidic channel and a screen-printed electrode; the micro-fluidic channel is used for collecting sweat; the screen-printed electrode comprises a working electrode for detecting glucose, a counter electrode and a reference electrode to form a three-electrode system; wherein the working electrode for detecting the glucose is provided with a functional layer, and the functional layer is modified by multi-shell metal organic framework layered encapsulation enzyme for detecting the glucose and chitosan for fixing the multi-shell metal organic framework layered encapsulation enzyme; the electrochemical detection module is used for obtaining electrochemical sensing detection data; and the iontophoresis module is used for outputting an adjustable constant current source to enable iontophoresis to stimulate skin to sweat. The portable long-time continuous detection of sweat glucose can be realized, and the detection method is rapid and convenient.

The invention discloses a smelting wastewater trace arsenic real-time monitoring method based on an electrochemical sensing array, and relates to the technical field of electrochemical detection.The method comprises the steps that bypass sample flow is established through a peristaltic pump and pre-filtered, and stable sample flow is obtained; the sample flow is divided into a first passage and a second passage, and a form distinguishing sample flow is obtained after oxidation setting and medium equalization; performing short-time adsorption equilibrium to obtain an interface state parameter set, executing multi-segment coded deposition control based on the parameter set, and collecting a dissolution current curve; a dissolution signal set is obtained by applying single-frequency perturbation signals, characteristic peak parameters are extracted and subjected to correlation calculation with a multi-section response matrix, the total arsenic concentration and the pentavalent arsenic concentration are calculated through a weighted inversion algorithm, and a confidence interval is obtained; and calculating the concentration of the trivalent arsenic and determining an uncertainty range according to the two. According to the method, the quality judgment result is linked with the dynamic adjustment of the sampling flow, the potential amplitude and the deposition duration, so that the self-adaptive optimization control of the detection process is realized.

The invention discloses an electrochemical detection method of dopamine, and belongs to the technical field of analysis. According to the method, SnS2 quantum dots are loaded on Zn-MOF and modified on an electrode, then gold nanoparticles and a sulfydryl-containing dopamine aptamer single chain are sequentially fixed on the electrode, an Apt / AuNPs / SnS2QDs (at) Zn-MOF / GCE composite electrode (in an open state) is formed, an electrochemical signal of the composite electrode is greatly improved, and based on specific recognition between dopamine and an aptamer chain, the electrochemical performance of the composite electrode is improved. A large cavity of Zn-MOF is used for packaging SnS2 quantum dots with excellent electrochemical properties, electrochemical signal amplification is achieved, when a target object dopamine exists, dopamine can quench an ECL signal, an electric signal is obviously reduced (in a closed state), and therefore a linear relation between the change of the electrochemical signal and the concentration of dopamine is constructed, and the electrochemical property of the electrochemical signal is improved. Therefore, high-sensitivity and high-selectivity detection of dopamine is realized.

The invention relates to the technical field of electrochemical sensors, and provides a multi-channel electrochemical detection electrode, an electrochemical sensor and a detection method.The multi-channel electrochemical detection electrode comprises an electrode substrate and a plurality of working electrodes arranged on the electrode substrate; the surfaces of the plurality of working electrodes are respectively modified with different types of metal nano-catalysts to detect different gas components, and the surfaces of the working electrodes are covered with PDMS hydrophobic layers to form a hydrophilic / hydrophobic channel separation structure. The surfaces of a plurality of working electrodes are modified with different types of metal nano-catalysts and covered with PDMS hydrophobic layers to form a hydrophilic / hydrophobic channel separation structure, and the multi-channel electrochemical detection electrode separates gas molecules through hydrophilic / hydrophobic channels, so that the cross reaction can be inhibited, and the cross sensitivity can be reduced.

The invention provides a rapid nondestructive quality detection method and device for aquatic products, and relates to the technical field of food safety detection, and the device comprises a spectral analysis module, a biosensing module, an electrochemical detection module, a data processing module, a data output module and a portable device. According to the rapid nondestructive quality detection method and device for the aquatic products, multi-index synchronous detection of freshness, drug residues and heavy metal content of the aquatic products is realized through fusion of spectral analysis, biosensing and electrochemical detection technologies.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

The invention relates to the technical field of electrochemical detection and food quality safety, and particularly discloses an edible oil rancidity degree rapid detection system based on an electrochemical sensor, comprising: a data acquisition module for receiving an original electrochemical response signal and performing format standardization, abnormal data rejection and integrity verification; the data preprocessing module adopts a statistical optimization algorithm to reduce noise, correct baseline drift, generate a unified dimension data set and complete stability verification; the feature analysis module is used for extracting rancidity related feature parameters through statistical correlation analysis, and carrying out fusion calculation after significance verification to obtain feature values representing rancidity; and the evaluation and judgment module inputs the characteristic value into a pre-training model, outputs a quantitative index through multivariate statistical calculation, dynamically sets a safety threshold in combination with the edible oil type, and outputs a rancidity judgment conclusion after comparison. The method depends on statistical data processing and analysis, the detection reliability and accuracy are improved, and the method is suitable for edible oil quality safety rapid screening and quality monitoring.

The invention discloses a method and equipment for rapidly detecting the content of progesterone in cow and sheep milk, and the method comprises the following steps: respectively collecting an optical signal, an electrochemical signal and current and voltage data of a sample in the cow and sheep milk through an optical detection module, an electrochemical detection module and a current and voltage detection module; the physical and chemical characteristics of the sample in the cow and sheep milk can be comprehensively analyzed from multiple dimensions. The detection data can be quickly and accurately processed and transmitted by combining a signal processing system and equipment, a reliable guarantee is provided for subsequent analysis, and in addition, a progesterone mathematical model embedded into the signal processing system realizes high-precision calculation of the concentration of progesterone in milk of cattle and sheep by combining a detection signal and a standard curve. The model not only improves the accuracy of a detection result, but also remarkably shortens the analysis time, and practically meets the urgent requirements for rapid detection and efficient output in practical application.

The invention provides a quality control solution and application thereof, the quality control solution comprises a buffer solution, a polymer, EDTA (Ethylene Diamine Tetraacetic Acid) or a salt thereof, a preservative, a blood simulation dye and an analyte, and the polymer is selected from PEG (Polyethylene Glycol) 2000-6000, propylene glycol, glycerol or a combination thereof. The quality control liquid provided by the invention can be used for quality control of at least one analyte; the kit does not need refrigeration, freezing or freeze-drying, can be placed at room temperature, is convenient to use, and can be used in the fields of immunodetection, biochemical detection and electrochemical detection.

The invention provides an electrode for monitoring active oxygen substances of tumor cells in real time, a detection system and a preparation method of the electrode. According to the electrode, an electrode array is constructed on a glass substrate through an MEMS technology, the electrode comprises a working electrode, a reference electrode and a counter electrode, the surface of the working electrode is modified with an AuNPs / ZIF-8 ZIF-67 nano-enzyme material so as to enhance the detection sensitivity of reactive oxygen species (ROS), and the detection system comprises the electrode, a PDMS cavity and an electrochemical detection device. The cell culture chamber is fixed on the electrode through plasma bonding and is provided with a through hole to be matched with the Transwell cell culture chamber, so that ROS released by incubated tumor cells can be directly diffused to the surface of the electrode, and efficient detection is realized. By optimizing the electrode modification material and the system structure, the detection sensitivity and stability are improved, and the sensor has real-time monitoring capability and is suitable for biomedical research and anti-cancer drug screening.

The invention discloses an online floating electrochemical scanning and electrospray ionization mass spectrometry combined device, relates to the technical field of analytical chemistry, and can improve the electrochemical detection efficiency and detection accuracy. According to the device, a three-electrode probe is arranged in a reaction cavity and is used for containing a target solution to be detected; the floating type electrochemical controller comprises a high-voltage module, a power supply module, a floating electrochemical measurement and control circuit and a three-electrode probe, the high-voltage module raises the first potential of the floating ground end of the power supply module into a second potential; the power supply module is used for converting the voltage of the floating battery into a preset voltage and then supplying power to the floating electrochemical measurement and control circuit; the floating electrochemical measurement and control circuit is used for controlling the three-electrode probe to output a continuous high-voltage signal or a continuous current signal, the continuous high-voltage signal or the continuous current signal acts on a target solution to generate an electrochemical reaction and directly generate spray ions, and the three-electrode probe is used for detecting voltage and current in the electrochemical reaction process of the target solution; and detection voltage and detection current are obtained.

The invention discloses a magnetic aggregation induced emission particle fluorescence-electrochemical nucleic acid dual detection chip and system.The chip comprises a chip body and an upper cover, and a plurality of electrochemical reaction detection cavities and fluorescence reaction detection cavities are formed in the left side and the right side of the chip body in the front-back direction respectively; the electrochemical reaction detection cavities and the fluorescence reaction detection cavities are arranged in a one-to-one correspondence manner; each electrochemical reaction detection cavity and each fluorescence reaction detection cavity are provided with a flow channel, and one end of each flow channel is communicated with the electrochemical reaction detection cavity or the fluorescence reaction detection cavity; the other end of the sampling tube is communicated with an MAIE electrochemical detection auxiliary liquid sample adding hole and an MAIE fluorescence detection auxiliary liquid sample adding hole which are formed in the chip main body; a plurality of MAIE electrochemical detection sample adding holes and MAIE fluorescence detection sample adding holes are formed in the upper cover, and corresponding openings are formed in the positions, corresponding to the MAIE electrochemical detection auxiliary liquid adding holes and the MAIE fluorescence detection auxiliary liquid adding holes, of the upper cover.

The invention relates to a samarium molybdate / reduced graphene oxide electro-catalytic material, a preparation method and application thereof and an electrochemical sensor, and relates to the technical field of electro-catalytic materials and electrochemical sensors, the preparation method comprises the following steps: dispersing GO in water, and stirring to obtain a GO suspension; the preparation method comprises the following steps: separately adding Sm (NO3) 3.6 H2O and Na2MoO4. 2H2O into water for ultrasonic dissolution, and mixing to obtain a first mixture; stirring and mixing the GO suspension and the first mixture to obtain a second mixture, and the like. The samarium molybdate / reduced graphene oxide electro-catalytic material is synthesized by a hydrothermal synthesis method and is used for modifying a glassy carbon electrode so as to be applied to electrochemical detection of FZD for the first time. Compared with a traditional detection method, the detection method has the advantages of being simple, efficient, green, environmentally friendly, sensitive in response and portable, and a new way is provided for detection of carcinogenic residues.

An electrochemical assay device includes an inner flow passage through which a liquid injected from an inlet flows and a first liquid absorbing material that absorbs the liquid that has passed through the inner flow passage, and is configured to be able to perform an assay based on an electrochemical method. The inner flow passage includes a microflow passage that communicates with the inlet and a separating flow passage provided between the microflow passage and the first liquid absorbing material for separating the liquid inside the inner flow passage into a part to be left in the microflow passage and a part to be absorbed by the first liquid absorbing material when the injection of the liquid is stopped. The electrochemical assay device includes an electrode portion that is disposed inside the microflow passage, a connecting portion that is connected to an external measurement device, and a conducting wire portion that electrically connects the electrode portion to the connecting portion.

The invention belongs to the technical field of electronic circuits, and particularly discloses a portable electrochemical detection device which comprises an excitation circuit, a constant potential circuit, an electrochemical sensor, a current-voltage conversion circuit and a low-pass filter circuit which are connected in sequence, the excitation circuit is used for generating control voltage; the constant potential circuit is used for applying stable bias voltage to a reference electrode and a counter electrode of the electrochemical sensor in combination with the control voltage, so that a working electrode of the electrochemical sensor generates a current signal corresponding to the concentration of an object to be detected; the current-voltage conversion circuit is used for converting the current signal into a voltage signal; and the low-pass filter circuit is used for filtering high-frequency noise in the voltage signal. The detection device has the advantages of wide range, adjustability, high precision, high accuracy, small size and the like, provides a new thought and method for trace harmful substance detection in the field of electrochemistry, and has wide application prospects in the fields of environmental monitoring, biomedical treatment, food safety and the like.

The invention provides a water environment heavy metal pollution measuring method based on electrochemical analysis. The method comprises the following steps: constructing a linear coefficient comparison set and a constant term comparison set of peak current and concentration of heavy metal ions on a certain stripping volt-ampere peak potential by aiming at different samples in a water environment detected sample set of a certain heavy metal; and then, by constructing a water environment similar sample set of the to-be-measured sample for heavy metal pollution measurement, the measurement concentration of the to-be-measured sample about the heavy metal ions is given. According to the technical scheme provided by the invention, the to-be-detected water environment sample is matched according to the data of a certain heavy metal ion of the detected water environment sample in the past, so that the adaptability of rapidly detecting the heavy metal ions of different water environment samples through the electrochemical detection device is enhanced.

The invention provides a method for enhancing an electrochemical detection signal of selenocysteine, which comprises the following steps: S1, mixing compound protease with selenocysteine with different concentrations in a ratio of 1: 1, and placing in a baking oven at 37 DEG C for 1 hour; s2, carrying out electrochemical detection on the selenocysteine on the surface of the electrode by adopting a square wave voltammetry to obtain an electrochemical signal of the selenocysteine; and S3, analyzing the electrochemical signal, and determining the concentration of selenocysteine. The selenocysteine is treated by the compound protease, the sensitivity of an electrochemical detection signal is remarkably enhanced, the current value generated by 1mu M of selenocysteine is 62nA under the condition that enzyme treatment is not carried out, and the current value of selenocysteine with the same concentration is remarkably increased to 137nA after the compound protease treatment, so that the sensitivity of the electrochemical detection signal is remarkably improved. The obvious current increase proves that the enzyme treatment is effective for improving the detection sensitivity of the selenocysteine, so that the existence of the selenocysteine can be accurately detected under the condition of low concentration.

The invention discloses a soil heavy metal monitoring device based on spectrum deep learning and an evaluation method, the soil heavy metal monitoring device comprises a plurality of column leaching containers, the plurality of column leaching containers are sequentially connected in series through hoses to form a percolation passage, the front end of the percolation passage is connected with a water outlet bucket through a peristaltic pump, and the rear end of the percolation passage is connected with a water inlet bucket; a branch pipe is led out of the hose between every two adjacent column leaching containers through a three-way pipe, the multiple branch pipes are connected with the multiple collection bottles respectively, and electrochemical detection assemblies are arranged on the collection bottles; the plurality of cuvette brackets are respectively arranged on the plurality of hoses, each cuvette bracket is connected with the optical fiber spectrometers through optical fibers, and the plurality of optical fiber spectrometers are electrically connected with the computer; the scheme aims at providing an effective evaluation method aiming at the soil pollution problem of a heavy metal high background area, the concentration of harmful heavy metal elements in the soil is remarkably reduced, the soil quality is improved, and important technical support and theoretical basis are provided for soil remediation.

The micro-fluidic chip comprises a base body, a first on-off switching piece, an electrochemical detection piece, a fluid driving piece, a first reagent storage piece and a second on-off switching piece, the base body is provided with an extraction chamber, a uniform mixing chamber, a reaction chamber, an electrochemical reaction tank and a waste liquid chamber, an outlet of the extraction chamber is communicated with an inlet of the uniform mixing chamber, and an outlet of the reaction chamber is communicated with an outlet of the uniform mixing chamber. An outlet of the uniform mixing chamber is communicated with an inlet of the reaction chamber through a first on-off switching piece, the outlet of the uniform mixing chamber is communicated with the waste liquid chamber through the first on-off switching piece, an outlet of the reaction chamber is communicated with an inlet of the electrochemical reaction tank through the first on-off switching piece, and an outlet of the electrochemical reaction tank is communicated with the waste liquid chamber; the electrochemical detection part is used for acquiring reaction information of the electrochemical reaction tank; the fluid driving part is connected with the base body and is communicated with the extraction chamber through the second on-off switching part; the first reagent storage part is arranged on the base body and is communicated with the extraction chamber through the second on-off switching part; and cross contamination is avoided.

The invention provides a micro-fluidic biosensor array, and relates to the technical field of sewage detection, the micro-fluidic biosensor array comprises a flow channel layer, a first detection layer and a second detection layer which are sequentially stacked from top to bottom; a flow channel structure is arranged in the flow channel layer, a micro-column array is arranged in the flow channel structure, and the micro-column array is used for generating a gradient resistance field for flowing liquid; the first detection layer is provided with a liquid inlet, the liquid inlet is used for receiving liquid discharged from the flow channel layer, the first detection layer comprises a three-dimensional interdigital electrode array and a micropore array modified by a quantum dot labeled antibody probe, and the three-dimensional interdigital electrode array is used for capturing and dissolving organic matter molecules; the second detection layer comprises a fluorescence detection module and an electrochemical detection module, the fluorescence detection module is used for carrying out cell counting and fluorescence signal acquisition on target microorganisms captured by the micropore array, and the electrochemical detection module is used for realizing directional enrichment and concentration detection of heavy metal ions. By means of the micro-fluidic biosensor array, sewage detection of multiple types of parameters can be achieved.

The invention discloses a system for detecting and analyzing water-soluble heavy metals in an ecological system based on machine learning. The system is used for detecting water-soluble harmful heavy metal elements in atmospheric particulates. The system comprises a sampling preprocessing module, an electrochemical detection module, a machine learning analysis module and a data verification output module which are connected in sequence, the sampling pretreatment module is used for collecting PM2.5 and PM10 particles by adopting a quartz fiber filter membrane and carrying out dissolving and filtering pretreatment; the electrochemical detection module is used for heavy metal enrichment and detection; the machine learning analysis module adopts an improved CNN (Convolutional Neural Network) to automatically identify the volt-ampere spectrogram, and establishes a nonlinear mapping model of the relationship between the metal ion concentration and the peak current; and the data verification output module performs quantitative analysis by adopting a standard addition method, and compares and identifies the pollution source through a multi-element fingerprint feature database. According to the invention, eight heavy metal elements such as Cd, V, Cr, Ni, Se, As, Mn and Pb can be detected at the same time, the detection range is 0.1-1000 [mu] g / L, and intelligence and automation of heavy metal detection are realized.

The invention belongs to the technical field of electrochemical detection, and discloses an anti-pollution electrochemical saliva sensor based on fluorinated side chain polypeptide as well as a preparation method and application of the anti-pollution electrochemical saliva sensor. The fluorinated side chain polypeptide disclosed by the invention not only has excellent hydrophilicity, can form a dense hydration layer with water molecules to repel pollutants, but also has low surface energy, and the interaction between the pollutants and an interface is remarkably reduced. Through the synergistic effect, the fluorinated side chain polypeptide has better anti-pollution performance compared with traditional zwitterionic polypeptide. The anti-pollution electrochemical sensor constructed on the basis of the fluorinated side chain polypeptide can be used for directly detecting CA15-3 in saliva, the saliva does not need to be pretreated, and the detection accuracy can be comparable with that of a commercial ELISA (Enzyme-Linked Immunosorbent Assay) method.

The invention discloses a total homocysteine (Hcy) electrochemical detection method based on metallothionein. The invention relates to the technical field of electrochemical detection. Firstly, metallothionein combined with metal ions is prepared, free Hcy reacts with the metallothionein combined with the metal ions, and the metal ions are released into a solution. And detecting an electrochemical response signal of the metal ions by utilizing an electrochemical analysis technology, making a standard curve of the Hcy concentration and the electrochemical response signal of the metal ions, repeating the operation on a sample to be detected to obtain an electrochemical response signal, and calculating the concentration of the Hcy in the sample according to the standard curve. Only metallothionein needs to be prepared, the metallothionein is small in molecular weight, stable in structure, easy to prepare and low in cost, the metal ion concentration is detected through the electrochemical analysis technology, the method has the advantages of being short in detection time and high in sensitivity, and rapid, accurate, high-sensitivity, simple and low-cost detection of clinical sample total homocysteine is achieved.

The invention belongs to the technical field of electrochemical detection sensors, and particularly relates to a photoelectrochemical sensor for dopamine concentration detection and a preparation method and application thereof. The photoelectrochemical sensor comprises a working electrode, a counter electrode and a reference electrode, and specifically, the working electrode, the counter electrode and the reference electrode are sequentially connected to an electrochemical workstation to obtain the photoelectrochemical sensor. The working electrode is an indium tin oxide electrode; a Bi4O5Br2 / g-C3N4 / Au composite material is deposited on the surface of the indium tin oxide electrode. The sensor provided by the invention is excellent in detection performance, has the advantages of simple preparation process, low cost, good environmental stability and the like, provides a reliable new method for clinical dopamine detection, and has an important application prospect in early diagnosis of neurodegenerative diseases such as Parkinson's disease and the like.

The invention belongs to the technical field of biochemical detection, and particularly relates to a bionic microsphere, a non-diagnostic-purpose extracellular vesicle detection method and an extracellular vesicle detection kit, the bionic microsphere is prepared by modifying a homologous cell membrane CM on the surface of a silica microsphere MS; the bionic microsphere (CMS) is used for capturing specific types of extracellular vesicles (EVs) in a to-be-detected sample and an optical / electrochemical detection method, can specifically capture homologous EVs, and is combined with fluorescence, ultraviolet visible absorption, differential pulse voltammetry and electrochemical luminescence for detection.