85 results about "Electrochemical detection" patented technology

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.

This invention discloses an Au / CeO2-BTC nanozyme based on strong metal-support interactions and its application in electrochemical glucose detection. The nanozyme uses a Ce-BTC metal-organic framework as a precursor, which is calcined to obtain a porous CeO2-BTC support rich in oxygen vacancies. Gold nanoparticles are then loaded onto the support via in-situ deposition. The product forms Au-O-Ce interfacial active sites and exhibits significant strong metal-support interactions, enabling electronic regulation and enhancing CeO2-support electrochemical detection. 3+ The content is increased and the charge transfer rate is accelerated. The electrode modified with this Au / CeO2-BTC nanozyme exhibits excellent catalytic performance for glucose oxidation, with a sensitivity of 41.3 μA·cm. ‑2 ·mM ‑1 The detection limit is as low as 4.75 μM, and it exhibits good reproducibility, stability, and selectivity.

This invention discloses a G4-DNA tetrahedral probe electrode, its preparation method, applications, and application methods. This invention utilizes four DNA strands to self-assemble into a DNA tetrahedral structure based on Watson-Crick hybridization. The DNA tetrahedron and G4 strand can be connected to the electrode based on the interaction between thiol groups and the gold electrode, and complementary base pairing. The addition of the DNA tetrahedron, through the construction of a reasonable probe density, further improves the detection sensitivity. This invention utilizes square wave voltammetry for electrochemical detection of malachite green. The content of malachite green can be determined based on the strength of the current signal of its characteristic peak. Rapid detection of malachite green can be achieved in a short time, with a minimum detection concentration of 3.65 μg / kg, showing potential for on-site detection. Six repeated tests using this electrode yielded almost identical detection signals, indicating that the electrode has good reusability and stability.

This invention discloses a functionalized biomodified electrode for rapid pathogen detection and its preparation method, relating to the fields of biosensing and electrochemical detection technology. It includes a glassy carbon electrode substrate, the surface of which is sequentially modified with a reduced graphene oxide layer, an electropolymerized polydopamine film, and an electrodeposited gold nanoparticle layer, forming a composite nanomaterial modified electrode. Antibodies are captured and fixed to the electrode surface through covalent interactions. This functionalized biomodified electrode for rapid pathogen detection and its preparation method, using the same electrochemical workstation, first electropolymerizes dopamine to form a film and then electrodeposits gold nanoparticles. This allows for precise control of film thickness, gold particle size, and coverage density by parameters such as polymerization potential, scan cycles, deposition potential, and deposition time. This avoids batch-to-batch fluctuations caused by dissolved oxygen and solution disturbances in traditional chemical methods, reducing the relative standard deviation of peak current detection from different batches of electrodes from 18.7% to 4.2%–4.5%.

The application discloses a preparation method of an Fe-NC@CNFs / GCE electrode and application thereof, relates to the technical field of electrochemical detection, and has the technical scheme as follows: a bimetallic ZIF based on Fe / Zn is synthesized, and is calcined at a high temperature of 800 DEG C to obtain a derived nitrogen-doped porous carbon material (Fe-NC@CNFs); the synthesized material has a large specific surface area and rich active sites; the material is configured into a suspension by using ultrapure water; and a traditional drop-coating method is adopted to successfully build an electrode Fe-NC@CNFs / GCE for detecting luteolin; the electrode has high selectivity, sensitivity and stability, and can detect luteolin in a natural sample.

This invention discloses an SFTSV virus detection kit and method based on LAMP-CRISPR / Cas combined with electrochemistry. By integrating multiple detection technologies, this invention combines the CRISPR / Cas system with LAMP isothermal amplification technology and electrochemical detection technology to achieve more efficient and sensitive nucleic acid detection, greatly shortening the detection time and meeting the needs of rapid clinical diagnosis. The detection exhibits high specificity and sensitivity, enabling accurate detection of samples with extremely low concentrations. The equipment is simplified and portable, facilitating real-time monitoring and automated operation. This invention innovatively combines real-time signal detection with automated operation, enabling real-time monitoring of the amplification process and direct acquisition of results through electrochemical signals, reducing operational difficulty and improving detection efficiency.

The application relates to the technical field of electrochemical detection, and specifically discloses an ammonium sulfate PH calculation method based on an electrochemical principle and a temperature compensation technology, which comprises the following steps: collecting a non-standard state electrode potential signal and an NTC thermistor real-time resistance signal of an ammonium sulfate solution to be measured; using an improved temperature fitting algorithm based on a temperature-resistance nonlinear relationship and an optimized fitting process to convert an actual thermodynamic temperature of the solution; combining the actual thermodynamic temperature to compensate the non-standard state electrode potential signal, eliminating the interference of temperature change on the potential measurement; and substituting the compensated electrode potential value and the thermodynamic temperature into a simplified expression of the Nernst equation to inversely solve the hydrogen ion concentration, and then obtaining the final PH value of the ammonium sulfate solution through negative logarithm operation. The method optimizes the temperature fitting precision and the electrode potential compensation adaptability, and improves the PH measurement deviation problem of the ammonium sulfate solution.

The present application relates to a kind of electrochemical detection method and system for capsaicin, detection method includes: providing three electrode system;Configuration electrolyte solution and dissolve capsaicin standard sample or sample to be measured;Electrochemical detection is carried out using three electrode system to obtain oxidation peak current signal;Based on the corresponding relationship between oxidation peak current and capsaicin concentration determines the capsaicin content in sample to be measured.Detection system includes three electrode system, battery module, power module, MCU module, electrochemical detection circuit, signal conditioning circuit and communication module, can realize the rapid, sensitive detection of capsaicin.The present application improves the detection sensitivity by carbon nanocomposite modified carbon cloth electrode, combined with portable hardware circuit design, get rid of the dependence on large instrument, applicable to the on-site rapid detection of capsaicin in food industry, provide scientific, objective technical means for hot degree quantification.

This invention discloses a method for preparing a nano-zinc sulfide composite material, comprising: (1) dissolving ammonium molybdate in deionized water, stirring evenly to form a 50 g / L solution, then sequentially adding zinc acetate and thiourea, wherein the mass ratio of ammonium molybdate to zinc acetate and thiourea is 2:1, and stirring thoroughly to obtain a mixed solution; (2) transferring the obtained mixed solution to a reaction vessel, reacting at a constant temperature of 160℃ for 12 h, and obtaining a solid powder after treatment; (3) heat-treating at 300-400℃ for 2 h to obtain the nano-zinc sulfide composite material. This invention synthesizes zinc sulfide nanomaterials with different sizes and morphologies, while molybdenum dioxide nanoparticles modify the surface of zinc sulfide. The two work synergistically to have heterogeneous structural characteristics that promote and accelerate electron transport. Using them as electrode materials for electrochemical detection of nicotine and the metabolite cotinine content in cigarettes shows potential application advantages.

The present invention relates to the electrochemical detection of an analyte by capacitance spectroscopy using a redox active polymer-coated electrode such as a polyaniline-coated electrode.

The application belongs to the technical field of sensor preparation, and particularly relates to an integrated interstitial fluid sampling and electrochemical detection system and a preparation method and application thereof. The integrated interstitial fluid sampling and electrochemical detection system comprises a microneedle array unit, an electrochemical sensor and a micro negative pressure pump. The microneedle array unit has good biocompatibility, is safe and reliable, and will not damage blood vessels and nerves of a patient; the microneedle is short and small, has weak discomfort, does not affect normal activities of a user, and is more conducive to continuous long-term monitoring; the electrochemical sensor can accurately identify a template molecule and transmit a signal; and the micro negative pressure pump can generate vacuum pressure to collect a sample amount meeting detection. The integrated interstitial fluid sampling and electrochemical detection system can be used by being inserted into a subcutaneous or intradermal position of a human body, can realize real-time and integrated sampling-detection, and can detect various biomarkers.

This invention relates to the field of electrochemical detection technology for vitamin E, specifically to an electrochemical detection device and method for vitamin E. The method includes: utilizing a detection system composed of a glassy carbon electrode, a saturated calomel electrode, and a platinum wire electrode; performing a series of optimizations and screenings to determine the SWV electrochemical detection method; and electrode pretreatment: using 0.05 μm alumina powder to perform circular polishing on the glassy carbon electrode in both directions for 3-4 minutes, followed by ultrasonic cleaning with anhydrous ethanol and pure water for three minutes each time. Finally, the electrode is scanned using the SWV method in a prepared buffer solution until it stabilizes. The beneficial effects are: this invention is convenient and simple to operate, has a simple formulation that greatly optimizes the detectable limit, has low manufacturing cost and is readily available, and requires no electrode modification; the lowest detectable concentration is far below the minimum normal range of vitamin E in human blood.

The present application relates to aldose reductase activity analysis technical field, specifically relates to a kind of aldose reductase activity and its inhibitory effect quantitative analysis method.Local substrate concentration regulation is constructed by dividing multiple detection segments, combining bypass sampling channel, electrochemical detection cavity and compensation injection point, and the substrate environment of each detection segment is maintained consistent;Enzyme-free reference channel parallel to microfluidic reaction channel is set, and the total absorbance change amount background is separated, to obtain the real enzymatic absorbance change amount;Based on sliding time window, extract local dynamic enzyme reaction rate, combined with wall adsorption correction to eliminate the influence of enzyme activity attenuation along the way, identify stable reaction interval through local fluctuation coefficient of variation, determine local half response time and local onset time;Local onset time is used as the integration starting point, to calculate comprehensive inhibition intensity, realize inhibitor onset order and inhibition strength spatial quantitative analysis, improve the accuracy, stability and spatial comparability of detection result.

This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.

The utility model discloses an electrochemical detection system and electrochemical reaction device. Electrochemical detection system includes: bearing unit, ultrasonic wave generating unit, signal generating unit, timing unit and electrode detection unit, bearing unit is used for bearing ultrasonic wave generating unit, signal generating unit and timing unit, bearing unit still includes stirring cup bearing area, and stirring cup bearing area bears stirring cup, signal generating unit includes high frequency signal output end and first timing signal receiving end, ultrasonic wave generating unit includes high frequency signal receiving end and ultrasonic wave generating signal output end, timing unit includes ultrasonic wave generating signal receiving end and first timing signal output end, ultrasonic wave generating unit drives the high frequency vibration of the solution to be measured in stirring cup according to high frequency signal, and outputs ultrasonic wave generating signal to make timing unit start timing, and electrode detection unit is used for stretching into the solution to be measured in stirring cup to carry out solution to be measured detection. Effectively improve the detection sensitivity of electrode detection unit.

This invention discloses a drug quantitative detection system based on electrochemical response sequence alignment, specifically relating to the field of electrochemical detection. The system includes: a sample introduction module for injecting the sample into a three-electrode unit in the detection cell, maintaining contact between the working electrode, reference electrode, and counter electrode and the sample during the interface establishment period after sample injection, to output an interface establishment segment current sequence; and a voltage excitation acquisition module for sequentially applying an interface establishment segment excitation voltage and a quantitative detection segment excitation voltage to the three-electrode unit, recording the corresponding current values ​​at sampling times to output a response sequence arranged in chronological order. By acquiring data on the interface establishment process and determining the response trajectory accordingly, different sample matrices are first distinguished before entering the corresponding sequence alignment path, mitigating the response meaning deviation problem caused by a unified detection path from the source, and relatively improving the consistency of quantitative results.

The application discloses a blood glucose detection electrode, a nucleic acid detection electrode and a preparation method. The vertical graphene is grown by a PECVD method and has a three-dimensional petal-like structure. The vertical graphene under the structure is more solid than planar graphene. The vertical graphene can be used for grafting of chemical groups such as carboxyl groups, amino groups, aldehyde groups and hydroxyl groups in a plasma mode. The groups are introduced by the method, the structure and the conductivity of the vertical graphene are not damaged, the nucleic acid and the antibody are more favorable to occupy active sites, more nucleic acid and antibody are loaded, the electrochemical detection signal is improved, and in addition, the step of sealing can be omitted, and interference is avoided. The method can realize industrial production.

The application discloses a one-step monoclonal antibody drug electrochemical detection biosensor and a preparation method and application thereof. The application designs and screens an affinity specific DNA aptamer hairpin probe and uses the same as a specific capture unit, then uses a gold-sulfur bond to fix the DNA aptamer hairpin probe on a screen-printed electrode surface, meanwhile, uses a self-assembly interaction between a metal and a mercapto group to fix a blocking agent introduced on the screen-printed electrode surface, and blocks the excess electrode surface reaction sites, so that the one-step monoclonal antibody drug electrochemical detection biosensor is obtained. The one-step monoclonal antibody drug electrochemical detection biosensor has the advantages of simple operation, high sensitivity, less non-specific adsorption, high capture efficiency, stable physical and chemical properties, strong specificity and good selectivity, and can be used for capturing antibody drugs in complex biological samples.

The utility model relates to a kind of creatinine electrochemical detection test paper, belong to detection equipment technical field, especially creatinine electrochemical detection equipment technical field.The utility model is equipped with two groups of detection electrode with non-closed frame electrode structure and two independent blood tank channels opened by double-sided adhesive layer, the reaction of creatinine in blood sample and the reaction of creatine are independently carried out in two blood tanks, creatinine and creatine signals in blood sample can be detected simultaneously without mutual interference, so that the detection of creatinine is more accurate.In addition, the compact multilevel test paper structure design of the utility model not only reduces the volume of test paper, but also the design and layout of reasonable blood tank channel, escape gas port and detection electrode can facilitate the precise use of detector.

The application discloses a chemical modified electrode for detecting furazolidone and an electrochemical detection method thereof, and relates to the technical field of electrochemical detection, and comprises the following steps: (1) preparation of longan carbon; (2) preparation of nitrogen-doped longan carbon material (NCs); (3) preparation of nitrogen-doped longan carbon-polydopamine material (NCs-PDA); (4) preparation of NCs-PDA / SPCE modified electrode; (5) electrochemical detection of FZD; and (6) characterization of NCs-PDA / SPCE. The chemical modified electrode for detecting furazolidone and the electrochemical detection method thereof use longan shells as a carbon source, chitosan and urea as nitrogen sources, and prepare a nitrogen-doped porous carbon material through a high-temperature carbonization method, combine polydopamine and a portable disposable screen-printed carbon electrode (SPCE), prepare an electrochemical modified electrode NCs-PDA / SPCE for detecting furazolidone, and establish an electrochemical rapid detection method for detecting furazolidone in animal-derived food based on the modified electrode.

The invention discloses an enzyme functionalized sensor for detecting nano plastic DPV in a biological matrix as well as a preparation method and a detection method of the enzyme functionalized sensor, and belongs to the field of nano plastic detection. The sensor comprises an electrode and CAT enzyme, wherein the surface of the electrode is modified with positively charged nano-gold particles, and the CAT enzyme is fixed through positive and negative electrostatic interaction. According to the sensor, the CAT enzyme and the nano plastic are combined to generate conformational change to change the activity of the CAT enzyme so as to cause DPV signal change, the sensitivity is high, the sensor has similar DPV signal response to different types of nano plastic, and a unified calibration model can be utilized to detect a complex biological matrix system so as to realize total amount determination of various nano plastic. And the electrochemical detection method does not need complex instruments and pretreatment, and provides a rapid, economic and extensible detection scheme for nano plastic exposure evaluation and environmental monitoring.

This invention belongs to the field of organic probe molecular analysis and detection technology, and discloses a fluorescence / electrochemical dual-mode probe for detecting copper ions (Cu2+), its preparation method, and its application. The probe's molecular structure is as follows: Under argon protection and an ice-salt bath, halogenated hydroxyl chloride and pyridinecarboxyl chloride hydrochloride undergo an esterification reaction in anhydrous dichloromethane solvent catalyzed by triethylamine. The target product is obtained after extraction, drying, vacuum concentration, and column chromatography purification. This probe combines "on-screen" fluorescence detection with ratiometric electrochemical detection, enabling highly sensitive and selective quantitative analysis of free Cu2+ in complex biological samples such as dialysis fluid from Alzheimer's disease (AD) model mice. Compared to existing detection technologies, this probe has advantages such as high selectivity, low detection limit, good stability, and accurate dual-signal cross-validation results, providing a reliable tool for the accurate detection of Cu2+ in biological samples.

The application relates to the technical field of intelligent devices, and discloses a wearable continuous dynamic uric acid intelligent monitoring device, which comprises a device body and is worn on a human body; a skin adhesion layer, a bionic micro-fluidic gel layer and an integrated ion electrophoresis electrode substrate are sequentially arranged on the device body from outside to inside; the skin adhesion layer is in contact with the human skin; the bionic micro-fluidic gel layer is used for collecting sweat on the human skin and guiding the sweat into a detection area on the bionic micro-fluidic gel layer; an electrochemical detection unit is arranged on the integrated ion electrophoresis electrode substrate; the electrochemical detection unit is used for detecting the sweat in the detection area; an analysis feedback module is arranged on the device body; and data detected by the electrochemical detection unit is fed back to a wearer after being analyzed by the analysis feedback module. The application can realize real-time dynamic monitoring of uric acid levels and meet the comfortable, convenient and accurate uric acid monitoring requirements of gout patients.

This invention relates to an online calibration method and system for wearable electrochemical sensors based on pollution state identification, belonging to the field of electrochemical detection and signal processing technology. By extracting the response distortion characteristics of the target analyte and auxiliary characterization components, a pollution state feature vector is constructed and input into a compensation and reconstruction model to output the equivalent response or correction parameters under a reference state. This allows for online correction of the current measurement results. When the correction parameters or reconstruction error exceed a preset safety threshold, automatic compensation is stopped, and physical recalibration or device maintenance prompts are triggered. This achieves high-precision online correction between two physical calibrations and extends the effective calibration cycle of the sensor.

The application discloses a kind of fresh wet noodles preservation plasma activation water cavitation system and method, the system includes high-frequency low-voltage driving power supply, embedded central processing unit, fluid pipeline, multistage cavitation reaction device, microchannel ozone generation device, mounting platform and magnetic control type gas flow proportional regulating valve.System utilizes microchannel discharge technology to generate ozone efficiently, and realizes multistage mixing and cavitation reaction of ozone and clean water by multistage alternating steady flow cavity and throat structure, to generate hydroxyl radical.Electrochemical detection unit integrated in the tail of system can monitor hydroxyl radical concentration on-line in real time, and embedded central processing unit dynamically regulates the opening of magnetic control valve according to monitoring data, to accurately adjust ozone dosage, to form closed-loop intelligent control.The application realizes the integration of hydroxyl radical generation, on-line sensing and process control, and the generated plasma activation water significantly improves the fresh wet noodles preservation capacity.

This utility model belongs to the technical field of electrochemical detection equipment, and provides a portable electrochemiluminescence detection device. It includes a dark box housing with a main unit compartment and a detection compartment, a photon counting unit fixedly installed in the main unit compartment, and an electrochemical pressure application unit fixedly installed in the detection compartment. A horizontal partition inside the dark box housing divides its internal space into a top main unit compartment and a bottom detection compartment. The horizontal partition has a detection through-hole connecting the main unit compartment and the detection compartment. The photon counting unit performs electrochemiluminescence detection on the sensor to be tested connected to the electrochemical pressure application unit through the detection through-hole. The portable electrochemiluminescence detection device provided by this utility model has a simple structure and is easy to use, reducing the operational difficulty and complexity of electrochemiluminescence detection, improving the efficiency of electrochemiluminescence detection, and is easy to carry and transport for on-site testing. It is highly practical and has a wide range of applications.

Sensor device with: a substrate (10); and at least one chemical or electrochemical detection device (12) formed on and / or in the substrate (10), which has a detection opening (14) structured in the substrate (10) and is configured such that the presence of at least one material to be detected in the detection opening (14) causes a change in a first sensor signal output by or tapped from the detection device (12); characterized in that the detection opening (14) comprises access openings (16) structured by a first side (10a) of the substrate (10) and a first cavity (18) into which the access openings (16) open, wherein the sensor device further comprises at least one additional sensing device (26) formed on and / or in the substrate (10), and wherein a second cavity (28) is formed in the substrate (10) adjacent to the sensing device (26),in which structured connecting openings (30) open through the first side (10a) of the substrate (10), and wherein the further sensing device (26) has a sensitive surface (34) located on a second side (10b) of the substrate (10) facing away from the first side (10a) and is configured such that a change in at least one physical quantity at the sensitive surface (34) causes a change in a second sensor signal output by or tapped from the sensing device (26), and wherein the further sensing device (26) is a pressure and / or sound detection device (26) which has a membrane component (36) that covers a third cavity (38) formed in the substrate (10) such that the membrane component (36) is activated by means of a pressure change at its sensitive surface (34) facing away from the third cavity (38) as the change in at least one physical quantity. or is bulgeable.

This invention provides an electrochemical detection cell and method for detecting carbohydrates. The method involves creating a first, second, and third flow channel on an auxiliary electrode, with the reference electrode cavity connected to both the second and third flow channels. Liquid enters the detection cell through the first flow channel, reacts there, then flows through the second flow channel into the reference electrode cavity, and finally exits through the third flow channel. This allows air bubbles within the reference electrode cavity to escape through the third flow channel, preventing bubble accumulation and potential control inaccuracies. Furthermore, the third flow channel is connected to the reference electrode cavity, enabling timely replacement of the eluent with fresh eluent when the eluent concentration changes or during dynamic gradient elution. This adaptability to different liquid phase environments improves detection accuracy.