614 results about "Electrochemical detection" patented technology

The invention discloses a power distribution network collaborative management method and system based on artificial intelligence, and relates to the technical field of electrochemical detection, and the method comprises the steps: constructing a distributed edge computing node network, deploying nodes at key positions of a power distribution network, achieving the collection and preprocessing of local power data, and reducing the cross-regional data transmission pressure; an AI real-time communication scheduling model is established based on the preprocessed data, communication resources are dynamically allocated according to the operation state of the power distribution network, and fault data transmission is guaranteed preferentially; seamless interaction of multi-protocol equipment is realized through a self-adaptive protocol conversion mechanism containing protocol identification, format conversion and data verification; training a fault diagnosis model by using a federated learning framework, and enabling edge nodes to only upload parameters to a coordination center for aggregation and updating, so as to balance model precision and data privacy; when a fault is detected, a millisecond response mechanism is started, and a processing strategy is generated and executed in combination with edge local decision and central global optimization.

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.

The invention discloses a liquid cooling charging module health state prediction system, and relates to the technical field of electrochemical detection, and the system comprises a multi-parameter collection unit which collects the pressure and flow data of a cooling liquid, and is provided with an independent collection node at each branch of a double-gun charging system, and provides basic data for monitoring; the dynamic calibration unit communicates with the acquisition unit, compensates data drift based on a temperature-vibration interference model of machine learning training, executes baseline calibration by using an idle period to update a reference value, and guarantees data accuracy; the leakage detection unit is used for calculating a data change rate after compensation, outputting an early warning according to a preset condition and positioning a leakage branch; and the health state evaluation unit fuses the early warning signal and the historical operation data to generate a prediction result. The problems that tiny leakage detection is difficult and a sensor is prone to interference can be solved, manual inspection is reduced, the maintenance cost is reduced, and the requirement of a high-power charging scene is met.

The invention discloses a smelting wastewater trace arsenic real-time monitoring method based on an electrochemical sensing array, and relates to the technical field of electrochemical detection.The method comprises the steps that bypass sample flow is established through a peristaltic pump and pre-filtered, and stable sample flow is obtained; the sample flow is divided into a first passage and a second passage, and a form distinguishing sample flow is obtained after oxidation setting and medium equalization; performing short-time adsorption equilibrium to obtain an interface state parameter set, executing multi-segment coded deposition control based on the parameter set, and collecting a dissolution current curve; a dissolution signal set is obtained by applying single-frequency perturbation signals, characteristic peak parameters are extracted and subjected to correlation calculation with a multi-section response matrix, the total arsenic concentration and the pentavalent arsenic concentration are calculated through a weighted inversion algorithm, and a confidence interval is obtained; and calculating the concentration of the trivalent arsenic and determining an uncertainty range according to the two. According to the method, the quality judgment result is linked with the dynamic adjustment of the sampling flow, the potential amplitude and the deposition duration, so that the self-adaptive optimization control of the detection process is realized.

The invention relates to the technical field of electrochemical sensors, and provides a multi-channel electrochemical detection electrode, an electrochemical sensor and a detection method.The multi-channel electrochemical detection electrode comprises an electrode substrate and a plurality of working electrodes arranged on the electrode substrate; the surfaces of the plurality of working electrodes are respectively modified with different types of metal nano-catalysts to detect different gas components, and the surfaces of the working electrodes are covered with PDMS hydrophobic layers to form a hydrophilic / hydrophobic channel separation structure. The surfaces of a plurality of working electrodes are modified with different types of metal nano-catalysts and covered with PDMS hydrophobic layers to form a hydrophilic / hydrophobic channel separation structure, and the multi-channel electrochemical detection electrode separates gas molecules through hydrophilic / hydrophobic channels, so that the cross reaction can be inhibited, and the cross sensitivity can be reduced.

The invention provides a rapid nondestructive quality detection method and device for aquatic products, and relates to the technical field of food safety detection, and the device comprises a spectral analysis module, a biosensing module, an electrochemical detection module, a data processing module, a data output module and a portable device. According to the rapid nondestructive quality detection method and device for the aquatic products, multi-index synchronous detection of freshness, drug residues and heavy metal content of the aquatic products is realized through fusion of spectral analysis, biosensing and electrochemical detection technologies.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

The invention relates to the technical field of electrochemical detection and food quality safety, and particularly discloses an edible oil rancidity degree rapid detection system based on an electrochemical sensor, comprising: a data acquisition module for receiving an original electrochemical response signal and performing format standardization, abnormal data rejection and integrity verification; the data preprocessing module adopts a statistical optimization algorithm to reduce noise, correct baseline drift, generate a unified dimension data set and complete stability verification; the feature analysis module is used for extracting rancidity related feature parameters through statistical correlation analysis, and carrying out fusion calculation after significance verification to obtain feature values representing rancidity; and the evaluation and judgment module inputs the characteristic value into a pre-training model, outputs a quantitative index through multivariate statistical calculation, dynamically sets a safety threshold in combination with the edible oil type, and outputs a rancidity judgment conclusion after comparison. The method depends on statistical data processing and analysis, the detection reliability and accuracy are improved, and the method is suitable for edible oil quality safety rapid screening and quality monitoring.

The invention provides an electrode for monitoring active oxygen substances of tumor cells in real time, a detection system and a preparation method of the electrode. According to the electrode, an electrode array is constructed on a glass substrate through an MEMS technology, the electrode comprises a working electrode, a reference electrode and a counter electrode, the surface of the working electrode is modified with an AuNPs / ZIF-8 ZIF-67 nano-enzyme material so as to enhance the detection sensitivity of reactive oxygen species (ROS), and the detection system comprises the electrode, a PDMS cavity and an electrochemical detection device. The cell culture chamber is fixed on the electrode through plasma bonding and is provided with a through hole to be matched with the Transwell cell culture chamber, so that ROS released by incubated tumor cells can be directly diffused to the surface of the electrode, and efficient detection is realized. By optimizing the electrode modification material and the system structure, the detection sensitivity and stability are improved, and the sensor has real-time monitoring capability and is suitable for biomedical research and anti-cancer drug screening.

The invention discloses a magnetic aggregation induced emission particle fluorescence-electrochemical nucleic acid dual detection chip and system.The chip comprises a chip body and an upper cover, and a plurality of electrochemical reaction detection cavities and fluorescence reaction detection cavities are formed in the left side and the right side of the chip body in the front-back direction respectively; the electrochemical reaction detection cavities and the fluorescence reaction detection cavities are arranged in a one-to-one correspondence manner; each electrochemical reaction detection cavity and each fluorescence reaction detection cavity are provided with a flow channel, and one end of each flow channel is communicated with the electrochemical reaction detection cavity or the fluorescence reaction detection cavity; the other end of the sampling tube is communicated with an MAIE electrochemical detection auxiliary liquid sample adding hole and an MAIE fluorescence detection auxiliary liquid sample adding hole which are formed in the chip main body; a plurality of MAIE electrochemical detection sample adding holes and MAIE fluorescence detection sample adding holes are formed in the upper cover, and corresponding openings are formed in the positions, corresponding to the MAIE electrochemical detection auxiliary liquid adding holes and the MAIE fluorescence detection auxiliary liquid adding holes, of the upper cover.

An electrochemical assay device includes an inner flow passage through which a liquid injected from an inlet flows and a first liquid absorbing material that absorbs the liquid that has passed through the inner flow passage, and is configured to be able to perform an assay based on an electrochemical method. The inner flow passage includes a microflow passage that communicates with the inlet and a separating flow passage provided between the microflow passage and the first liquid absorbing material for separating the liquid inside the inner flow passage into a part to be left in the microflow passage and a part to be absorbed by the first liquid absorbing material when the injection of the liquid is stopped. The electrochemical assay device includes an electrode portion that is disposed inside the microflow passage, a connecting portion that is connected to an external measurement device, and a conducting wire portion that electrically connects the electrode portion to the connecting portion.

The invention provides a water environment heavy metal pollution measuring method based on electrochemical analysis. The method comprises the following steps: constructing a linear coefficient comparison set and a constant term comparison set of peak current and concentration of heavy metal ions on a certain stripping volt-ampere peak potential by aiming at different samples in a water environment detected sample set of a certain heavy metal; and then, by constructing a water environment similar sample set of the to-be-measured sample for heavy metal pollution measurement, the measurement concentration of the to-be-measured sample about the heavy metal ions is given. According to the technical scheme provided by the invention, the to-be-detected water environment sample is matched according to the data of a certain heavy metal ion of the detected water environment sample in the past, so that the adaptability of rapidly detecting the heavy metal ions of different water environment samples through the electrochemical detection device is enhanced.

The invention provides a micro-fluidic biosensor array, and relates to the technical field of sewage detection, the micro-fluidic biosensor array comprises a flow channel layer, a first detection layer and a second detection layer which are sequentially stacked from top to bottom; a flow channel structure is arranged in the flow channel layer, a micro-column array is arranged in the flow channel structure, and the micro-column array is used for generating a gradient resistance field for flowing liquid; the first detection layer is provided with a liquid inlet, the liquid inlet is used for receiving liquid discharged from the flow channel layer, the first detection layer comprises a three-dimensional interdigital electrode array and a micropore array modified by a quantum dot labeled antibody probe, and the three-dimensional interdigital electrode array is used for capturing and dissolving organic matter molecules; the second detection layer comprises a fluorescence detection module and an electrochemical detection module, the fluorescence detection module is used for carrying out cell counting and fluorescence signal acquisition on target microorganisms captured by the micropore array, and the electrochemical detection module is used for realizing directional enrichment and concentration detection of heavy metal ions. By means of the micro-fluidic biosensor array, sewage detection of multiple types of parameters can be achieved.

The invention discloses a system for detecting and analyzing water-soluble heavy metals in an ecological system based on machine learning. The system is used for detecting water-soluble harmful heavy metal elements in atmospheric particulates. The system comprises a sampling preprocessing module, an electrochemical detection module, a machine learning analysis module and a data verification output module which are connected in sequence, the sampling pretreatment module is used for collecting PM2.5 and PM10 particles by adopting a quartz fiber filter membrane and carrying out dissolving and filtering pretreatment; the electrochemical detection module is used for heavy metal enrichment and detection; the machine learning analysis module adopts an improved CNN (Convolutional Neural Network) to automatically identify the volt-ampere spectrogram, and establishes a nonlinear mapping model of the relationship between the metal ion concentration and the peak current; and the data verification output module performs quantitative analysis by adopting a standard addition method, and compares and identifies the pollution source through a multi-element fingerprint feature database. According to the invention, eight heavy metal elements such as Cd, V, Cr, Ni, Se, As, Mn and Pb can be detected at the same time, the detection range is 0.1-1000 [mu] g / L, and intelligence and automation of heavy metal detection are realized.

The invention belongs to the technical field of electrochemical detection, and discloses an anti-pollution electrochemical saliva sensor based on fluorinated side chain polypeptide as well as a preparation method and application of the anti-pollution electrochemical saliva sensor. The fluorinated side chain polypeptide disclosed by the invention not only has excellent hydrophilicity, can form a dense hydration layer with water molecules to repel pollutants, but also has low surface energy, and the interaction between the pollutants and an interface is remarkably reduced. Through the synergistic effect, the fluorinated side chain polypeptide has better anti-pollution performance compared with traditional zwitterionic polypeptide. The anti-pollution electrochemical sensor constructed on the basis of the fluorinated side chain polypeptide can be used for directly detecting CA15-3 in saliva, the saliva does not need to be pretreated, and the detection accuracy can be comparable with that of a commercial ELISA (Enzyme-Linked Immunosorbent Assay) method.

The invention belongs to the technical field of electrochemical detection sensors, and particularly relates to a photoelectrochemical sensor for dopamine concentration detection and a preparation method and application thereof. The photoelectrochemical sensor comprises a working electrode, a counter electrode and a reference electrode, and specifically, the working electrode, the counter electrode and the reference electrode are sequentially connected to an electrochemical workstation to obtain the photoelectrochemical sensor. The working electrode is an indium tin oxide electrode; a Bi4O5Br2 / g-C3N4 / Au composite material is deposited on the surface of the indium tin oxide electrode. The sensor provided by the invention is excellent in detection performance, has the advantages of simple preparation process, low cost, good environmental stability and the like, provides a reliable new method for clinical dopamine detection, and has an important application prospect in early diagnosis of neurodegenerative diseases such as Parkinson's disease and the like.

The invention provides a urine HCG full-automatic detection and urine routine combined detection system, which comprises a host shell, an interactive interface, a sample interface, a USB interface, a sampling and sample introduction module, a urine detection module, a urine detection module, a urine detection module and a urine routine detection module, the microfluidic and reagent module is used for automatically distributing and reacting urine samples; the multifunctional automatic detection module is used for performing optical detection and electrochemical detection and completing combined detection of HCG and urine routine; the data processing module is used for collecting original signals and carrying out data processing and calculation to obtain HCG concentration, urine routine multi-parameter results and a detection report; and the intelligent diagnosis support module is used for performing medical correlation analysis and medical record intelligent diagnosis. Through combined detection of HCG and urine routine, HCG and urine routine results can be obtained at the same time, the detection efficiency is improved, and the detection accuracy and sensitivity are enhanced through optical detection and electrochemical detection processes.

The invention belongs to the field of new materials, and discloses an electrode-based liposome adriamycin entrapment rate electrochemical detection method. According to the detection method, after a bipolar vertical ordered mesoporous silica film (bp-VMSF) is modified on an electrically activated glassy carbon electrode (p-GCE), separation, enrichment and electrochemical detection are integrated, and rapid and sensitive detection of adriamycin can be realized. The glassy carbon electrode is subjected to electrochemical activation pretreatment under the alkaline condition, bp-VMSF stably grows, the surface of the inner layer close to the p-GCE electrode is negatively charged, and the surface of the outer layer away from the electrode is positively charged. The asymmetric charge configuration enhances the adsorption capacity of positively charged adriamycin molecules on an electrode interface through electrostatic enrichment. The p-GCE adsorbs adriamycin and promotes electron transfer, and is combined with the synergistic enrichment effect of the bp-VMSF double-static nano-channel, so that the electrochemical response performance to adriamycin is jointly improved, a method is provided for rapid detection of the liposome adriamycin encapsulation efficiency, and the p-GCE has a good prospect in evaluation of liposome adriamycin preparations.

The invention provides a method for detecting chloramphenicol residues in animal food by adopting a molecular imprinting sensor, which comprises the following steps: preparing the molecular imprinting sensor with specific recognition on CAP, and providing an electrochemical detection system of the molecular imprinting sensor for detecting the chloramphenicol residues in the animal food, fitting to obtain a calculation formula of chloramphenicol concentration detected by the molecularly imprinted sensor; treating an animal food sample, and mixing the animal food sample with a standard concentration CAP solution according to a volume ratio of 1: 9 to obtain a to-be-detected sample solution; scanning the to-be-detected sample liquid through the molecular imprinting sensor by adopting a square wave voltammetry method to obtain a peak current value; and obtaining the concentration of chloramphenicol in the sample solution to be detected according to the peak current value and a calculation formula of chloramphenicol concentration detected by the molecular imprinting sensor. The method is sensitive, accurate and practical.

The utility model discloses a multifunctional blood glucose detector for detecting glycosylated hemoglobin, which comprises a shell, a control panel, a screen, a battery pack and a multifunctional detection mechanism, the multifunctional detection mechanism is provided with an electrochemical detection assembly and a photochemical detection assembly which can be used for performing electrochemical detection and photochemical detection on the test strip respectively, and the screen, the battery pack and the multifunctional detection mechanism are electrically connected with the control panel respectively; a user can directly complete electrochemical detection and photochemical detection on one machine in sequence, the operation is convenient, the efficiency is high, and the detection accuracy of glycosylated hemoglobin can be improved by utilizing dual detection.

The invention discloses a cell viability detection system and method.The cell viability detection system comprises a micro-fluidic chip module, an optical detection module, an electrochemical detection module, a data processing and analyzing module and a control module, and the micro-fluidic chip module is used for monitoring physical and chemical signal changes related to cell viability in real time; compared with the prior art, the method has the following advantages that optical and electrochemical detection technologies are combined, cell activity information is obtained from multiple dimensions, the accuracy and reliability of cell activity detection are remarkably improved through comprehensive analysis, the real cell activity state can be reflected more accurately, and the accuracy and reliability of cell activity detection are improved. The micro-fluidic chip technology realizes rapid mixing and reaction of cells and reagents, and meanwhile, the automatic detection and data processing flow greatly shortens the detection time, improves the detection efficiency and meets the high-throughput detection requirement.

The invention provides an in-situ electrochemical nuclear magnetic resonance and online differential mass spectrometry combined method and device, and relates to the field of electrochemical detection. The method comprises the following steps: establishing a gas inlet passage and a gas outlet passage on an electrochemical nuclear magnetic resonance in-situ pool; an electrochemical reaction process in the electrochemical nuclear magnetic resonance in-situ pool is controlled through the electrochemical workstation, and electrochemical signals are collected; inert gas is introduced into an occurrence place of the electrochemical reaction process through the gas inlet channel, gas generated in the electrochemical reaction process is introduced into the mass spectrometer from the gas outlet channel along with the inert gas, and a mass spectrum signal is collected; nuclear magnetic resonance signals are collected through a nuclear magnetic resonance spectrometer. In the device, an electrochemical nuclear magnetic resonance in-situ pool assembly comprises a counter electrode binding post, a shell, a micrometer head and a working electrode binding post; an air inlet channel and an air outlet channel are formed in the micrometer head. According to the invention, an electrochemical signal, a nuclear magnetic resonance signal and a mass spectrum signal can be monitored at the same time, and real-time synchronous detection of solid, liquid and gas phases is realized.

The invention discloses a high-throughput electrochemical detection platform based on an ultra-infiltration micro-droplet array, a preparation method of the high-throughput electrochemical detection platform and application of the high-throughput electrochemical detection platform in AD marker detection, and belongs to the technical field of intersection of analytical chemistry, biosensing and material science. The method comprises the following steps: cleaning and drying a substrate, and immersing the surface of the substrate in a fluorosilane solution to form a super-hydrophobic layer by adopting a dip-coating method; after covering with a mask plate, etching with O2 plasma so as to form a hydrophilic microdot matrix; respectively printing an Ag / AgCl reference electrode, a gold working electrode and a carbon counter electrode on the hydrophilic microdots; electrode wires are printed layer by layer, a reference electrode wire, a working electrode wire and a counter electrode wire are sequentially printed in the area outside the hydrophilic microdot matrix from bottom to top in a spin-coating PDMS layer separation mode after each layer of electrode wire is printed, and PDMS is spin-coated above the counter electrode wire for packaging after printing is completed; and on each gold working electrode, respectively modifying capture antibodies of A beta 42, A beta 40, P-tau181 and P-tau217 proteins through a sample application instrument, so as to obtain the detection kit.

The invention provides construction of an electrochemical biosensor based on a fucoidin hydrogel microneedle and application of the electrochemical biosensor in online monitoring of uric acid, and relates to the technical field of biosensors. According to the micro-needle, Gantrez S-97 and PEG are subjected to copolymerization crosslinking to form a hydrogel main body, fucoidin is introduced for blending modification, a three-dimensional network structure is constructed by utilizing a high-temperature esterification reaction for regulating and controlling pH, and the super-expansion hydrogel micro-needle with high swelling property and good biocompatibility is formed. The microneedle can rapidly collect a skin interstitial fluid sample, and then through a screen printing electrode (SPE) platform, carboxylated MWCNTs are used for modifying an electrode and immobilizing uricase to construct an enzyme sensor. Hydrogen peroxide generated by catalyzing uric acid on the surface of the electrode participates in electrochemical reaction, and an output signal is used for real-time detection of uric acid concentration. The device integrates minimally invasive sampling and high-sensitivity electrochemical detection, has the advantages of quick response, simplicity and convenience in operation, good biocompatibility and the like, and is suitable for application scenes of continuous monitoring of uric acid, chronic disease management and the like.

The invention discloses preparation and application of a gold electrode and an electrochemical detection device. The preparation method of the modified gold electrode comprises the following steps: activating a polished and ground bare gold electrode with nitric acid, and immersing the polished and ground bare gold electrode into a deoxidized cysteamine (Cyst) solution for 2 hours; washing with double distilled water and soaking for about 12 hours, and removing physically adsorbed Cyst to obtain a Cyst modified gold electrode; immersing the Cyst modified gold electrode into a nanogold solution for 18 hours, taking out and airing to obtain a colloidal gold-Cyst modified gold electrode; and finally, immersing the colloidal gold-Cyst modified gold electrode into Cyst for 2 hours, taking out and airing to obtain the target modified gold electrode. The modified gold electrode prepared by the method, a platinum wire electrode and a silver-silver chloride electrode form a three-electrode system. The modified gold electrode in the three-electrode system has a good electrochemical catalytic oxidation function on the uric acid, the peak current and the content of the uric acid are in a linear relation, and the system can be directly used for detecting the uric acid in the urine.

The invention discloses an AI-enabled real-time electrochemical detection system, which is composed of a real-time electrochemical detection module and an embedded AI data processing and analysis module, the real-time electrochemical detection module comprises an electrochemical electrode system, a sample pool and an electrochemical workstation, the surface of a working electrode is modified with a probe of a specific recognition target, and when the probe is combined with the target, the probe of the specific recognition target can be detected by the embedded AI data processing and analysis module. The working electrode interface charge transfer impedance changes in real time to generate a dynamic electric signal, and the electrochemical workstation provides electric input and collects the signal; the embedded AI data processing and analysis module is used for denoising the dynamic electric signals through an adaptive filtering algorithm based on wavelet transform, extracting time-frequency domain joint features through a 1D-CNN and LSTM mixed deep learning model, and establishing a nonlinear mapping relation between the signals and target concentration. Through AI enabling electrochemical detection, the defects of low time efficiency, large error and low information utilization rate of the traditional technology are comprehensively overcome, and an innovative solution is provided for high-precision, rapid and anti-interference target substance detection.

The utility model discloses an electrochemical reaction tank for a hard X-ray absorption spectrum in-situ dual-mode test. The electrochemical reaction tank comprises a reaction tank body, a working electrode, a reference electrode and a graphite counter electrode, the front plate is provided with a working electrode window, and the upper plate is provided with a reference electrode hole and a graphite counter electrode hole. The working electrode is arranged at the window of the front plate and is tightly attached to the reaction tank body through a polyimide adhesive tape, and the reference electrode and the graphite counter electrode are arranged on the two sides of the reaction tank body and are respectively connected with an external electrochemical working platform. The distance between the working electrode window and the rear plate is controlled, and the working electrode is coupled with synchrotron radiation X-ray incident light, a fluorescence signal and a transmission signal to form an X-ray dual-mode test channel. The advantages of a synchrotron radiation X-ray absorption spectrum measuring platform are fully utilized, a real in-situ electrochemical detection environment is simulated, and reliable fluorescence and transmission signals are collected at the same time.

The invention relates to a dual-mode paper-based sweat chloride ion sensor, a preparation method and application of the dual-mode paper-based sweat chloride ion sensor in rapid diagnosis of cystic fibrosis, and belongs to the crossing field of a biomedical detection technology and electrochemical / optical sensing materials and devices. The dual-mode paper-based sweat chloride ion sensor is composed of a colorimetric detection module and an electrochemical detection module. According to the invention, by optimizing the collaborative design of the colorimetric module and the electrochemical module and combining the integrated process of the silver nitrate / potassium chromate sensitive layer and the screen-printed electrode, the detection sensitivity limit is remarkably broken through, and the integrated process from preliminary screening to clinical definite diagnosis is realized. More importantly, a low-cost cellulose substrate and a silk-screen printing process are adopted, the single-time detection cost is lower than 0.1 dollar, the 180-second quick response characteristic is combined, the method completely meets the economical, practical, high-sensitivity, good-specificity, simple and quick to operate and repeatable diagnosis standards proposed by WHO, and the method is suitable for instant detection scenes of basic medical institutions.

The invention belongs to the field of bacterial detection, and discloses a fluorescence electrochemical detection product based on DNA Walker and asymmetric CRISPR (clustered regularly interspaced short palindromic repeats), which comprises a magnetic bead modified with a Lock chain, a magnetic bead modified with a Walker-Reporter chain, and an electrode of which the surface is modified with ssDNA-HRP. Two signal output modes of asymmetric CRISPR (clustered regularly interspaced short palindromic repeats) technology fluorescence display and electrochemical sensing technology are adopted for detection, and due to fluorescence and electrochemical dual-mode signal output, the false positive problem existing in a single mode is prevented, and rapid and sensitive detection of staphylococcus aureus is realized. The invention further discloses a construction method and application of the detection product, the detection product can be widely applied to detection of pollution of staphylococcus aureus in the environment, detection is rapid and sensitive, and detection of staphylococcus aureus is specific, accurate and reliable.

The invention relates to the technical field of electrochemical detection, in particular to a preparation method of a polydopamine modified nitrogen-doped carbon material and application of the polydopamine modified nitrogen-doped carbon material in electrochemical analysis and detection of copper ions. The preparation method comprises the following steps: calcining a Zn-MOF material in an inert atmosphere, removing metal zinc to obtain a porous nitrogen-doped carbon material with a hexagonal structure, mixing the nitrogen-doped carbon material with dopamine, and carrying out oxidative polymerization on the dopamine under an alkaline condition to obtain the polydopamine-modified nitrogen-doped carbon material. The polydopamine-modified nitrogen-doped carbon material prepared by the method has high electrochemical activity, rich active sites and excellent adsorption performance, is used for modifying a glassy carbon electrode to detect Cu < 2 + >, has high sensitivity to Cu < 2 + >, and overcomes the technical defects of electrode materials in the prior art.