448 results about "Electrochemical detection" patented technology

The invention discloses a power distribution network collaborative management method and system based on artificial intelligence, and relates to the technical field of electrochemical detection, and the method comprises the steps: constructing a distributed edge computing node network, deploying nodes at key positions of a power distribution network, achieving the collection and preprocessing of local power data, and reducing the cross-regional data transmission pressure; an AI real-time communication scheduling model is established based on the preprocessed data, communication resources are dynamically allocated according to the operation state of the power distribution network, and fault data transmission is guaranteed preferentially; seamless interaction of multi-protocol equipment is realized through a self-adaptive protocol conversion mechanism containing protocol identification, format conversion and data verification; training a fault diagnosis model by using a federated learning framework, and enabling edge nodes to only upload parameters to a coordination center for aggregation and updating, so as to balance model precision and data privacy; when a fault is detected, a millisecond response mechanism is started, and a processing strategy is generated and executed in combination with edge local decision and central global optimization.

The invention relates to a bioelectrochemical sensor used for detecting hydrogen peroxide and a manufacturing method of the bioelectrochemical sensor. The novel bioelectrochemical sensor is a sensor with a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is saturated calomel electrode and the working electrode is gold electrode. The sensor is based on orientation combination of a polypeptide horse radish peroxidase with characteristic sequence, the promotion effect of the polypeptide horse radish peroxidase on the activity of the enzyme and the characteristic of high sensitivity of electrochemical detection methods. The sensitivity of hydrogen peroxide sensors is effectively improved and satisfactory results are obtained.

The invention discloses a liquid cooling charging module health state prediction system, and relates to the technical field of electrochemical detection, and the system comprises a multi-parameter collection unit which collects the pressure and flow data of a cooling liquid, and is provided with an independent collection node at each branch of a double-gun charging system, and provides basic data for monitoring; the dynamic calibration unit communicates with the acquisition unit, compensates data drift based on a temperature-vibration interference model of machine learning training, executes baseline calibration by using an idle period to update a reference value, and guarantees data accuracy; the leakage detection unit is used for calculating a data change rate after compensation, outputting an early warning according to a preset condition and positioning a leakage branch; and the health state evaluation unit fuses the early warning signal and the historical operation data to generate a prediction result. The problems that tiny leakage detection is difficult and a sensor is prone to interference can be solved, manual inspection is reduced, the maintenance cost is reduced, and the requirement of a high-power charging scene is met.

The invention discloses a smelting wastewater trace arsenic real-time monitoring method based on an electrochemical sensing array, and relates to the technical field of electrochemical detection.The method comprises the steps that bypass sample flow is established through a peristaltic pump and pre-filtered, and stable sample flow is obtained; the sample flow is divided into a first passage and a second passage, and a form distinguishing sample flow is obtained after oxidation setting and medium equalization; performing short-time adsorption equilibrium to obtain an interface state parameter set, executing multi-segment coded deposition control based on the parameter set, and collecting a dissolution current curve; a dissolution signal set is obtained by applying single-frequency perturbation signals, characteristic peak parameters are extracted and subjected to correlation calculation with a multi-section response matrix, the total arsenic concentration and the pentavalent arsenic concentration are calculated through a weighted inversion algorithm, and a confidence interval is obtained; and calculating the concentration of the trivalent arsenic and determining an uncertainty range according to the two. According to the method, the quality judgment result is linked with the dynamic adjustment of the sampling flow, the potential amplitude and the deposition duration, so that the self-adaptive optimization control of the detection process is realized.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

The invention relates to the technical field of electrochemical detection and food quality safety, and particularly discloses an edible oil rancidity degree rapid detection system based on an electrochemical sensor, comprising: a data acquisition module for receiving an original electrochemical response signal and performing format standardization, abnormal data rejection and integrity verification; the data preprocessing module adopts a statistical optimization algorithm to reduce noise, correct baseline drift, generate a unified dimension data set and complete stability verification; the feature analysis module is used for extracting rancidity related feature parameters through statistical correlation analysis, and carrying out fusion calculation after significance verification to obtain feature values representing rancidity; and the evaluation and judgment module inputs the characteristic value into a pre-training model, outputs a quantitative index through multivariate statistical calculation, dynamically sets a safety threshold in combination with the edible oil type, and outputs a rancidity judgment conclusion after comparison. The method depends on statistical data processing and analysis, the detection reliability and accuracy are improved, and the method is suitable for edible oil quality safety rapid screening and quality monitoring.

The invention provides a water environment heavy metal pollution measuring method based on electrochemical analysis. The method comprises the following steps: constructing a linear coefficient comparison set and a constant term comparison set of peak current and concentration of heavy metal ions on a certain stripping volt-ampere peak potential by aiming at different samples in a water environment detected sample set of a certain heavy metal; and then, by constructing a water environment similar sample set of the to-be-measured sample for heavy metal pollution measurement, the measurement concentration of the to-be-measured sample about the heavy metal ions is given. According to the technical scheme provided by the invention, the to-be-detected water environment sample is matched according to the data of a certain heavy metal ion of the detected water environment sample in the past, so that the adaptability of rapidly detecting the heavy metal ions of different water environment samples through the electrochemical detection device is enhanced.

The invention discloses a system for detecting and analyzing water-soluble heavy metals in an ecological system based on machine learning. The system is used for detecting water-soluble harmful heavy metal elements in atmospheric particulates. The system comprises a sampling preprocessing module, an electrochemical detection module, a machine learning analysis module and a data verification output module which are connected in sequence, the sampling pretreatment module is used for collecting PM2.5 and PM10 particles by adopting a quartz fiber filter membrane and carrying out dissolving and filtering pretreatment; the electrochemical detection module is used for heavy metal enrichment and detection; the machine learning analysis module adopts an improved CNN (Convolutional Neural Network) to automatically identify the volt-ampere spectrogram, and establishes a nonlinear mapping model of the relationship between the metal ion concentration and the peak current; and the data verification output module performs quantitative analysis by adopting a standard addition method, and compares and identifies the pollution source through a multi-element fingerprint feature database. According to the invention, eight heavy metal elements such as Cd, V, Cr, Ni, Se, As, Mn and Pb can be detected at the same time, the detection range is 0.1-1000 [mu] g / L, and intelligence and automation of heavy metal detection are realized.

The invention belongs to the technical field of electrochemical detection, and discloses an anti-pollution electrochemical saliva sensor based on fluorinated side chain polypeptide as well as a preparation method and application of the anti-pollution electrochemical saliva sensor. The fluorinated side chain polypeptide disclosed by the invention not only has excellent hydrophilicity, can form a dense hydration layer with water molecules to repel pollutants, but also has low surface energy, and the interaction between the pollutants and an interface is remarkably reduced. Through the synergistic effect, the fluorinated side chain polypeptide has better anti-pollution performance compared with traditional zwitterionic polypeptide. The anti-pollution electrochemical sensor constructed on the basis of the fluorinated side chain polypeptide can be used for directly detecting CA15-3 in saliva, the saliva does not need to be pretreated, and the detection accuracy can be comparable with that of a commercial ELISA (Enzyme-Linked Immunosorbent Assay) method.

The invention belongs to the technical field of electrochemical detection sensors, and particularly relates to a photoelectrochemical sensor for dopamine concentration detection and a preparation method and application thereof. The photoelectrochemical sensor comprises a working electrode, a counter electrode and a reference electrode, and specifically, the working electrode, the counter electrode and the reference electrode are sequentially connected to an electrochemical workstation to obtain the photoelectrochemical sensor. The working electrode is an indium tin oxide electrode; a Bi4O5Br2 / g-C3N4 / Au composite material is deposited on the surface of the indium tin oxide electrode. The sensor provided by the invention is excellent in detection performance, has the advantages of simple preparation process, low cost, good environmental stability and the like, provides a reliable new method for clinical dopamine detection, and has an important application prospect in early diagnosis of neurodegenerative diseases such as Parkinson's disease and the like.

The invention provides a urine HCG full-automatic detection and urine routine combined detection system, which comprises a host shell, an interactive interface, a sample interface, a USB interface, a sampling and sample introduction module, a urine detection module, a urine detection module, a urine detection module and a urine routine detection module, the microfluidic and reagent module is used for automatically distributing and reacting urine samples; the multifunctional automatic detection module is used for performing optical detection and electrochemical detection and completing combined detection of HCG and urine routine; the data processing module is used for collecting original signals and carrying out data processing and calculation to obtain HCG concentration, urine routine multi-parameter results and a detection report; and the intelligent diagnosis support module is used for performing medical correlation analysis and medical record intelligent diagnosis. Through combined detection of HCG and urine routine, HCG and urine routine results can be obtained at the same time, the detection efficiency is improved, and the detection accuracy and sensitivity are enhanced through optical detection and electrochemical detection processes.

The invention belongs to the field of new materials, and discloses an electrode-based liposome adriamycin entrapment rate electrochemical detection method. According to the detection method, after a bipolar vertical ordered mesoporous silica film (bp-VMSF) is modified on an electrically activated glassy carbon electrode (p-GCE), separation, enrichment and electrochemical detection are integrated, and rapid and sensitive detection of adriamycin can be realized. The glassy carbon electrode is subjected to electrochemical activation pretreatment under the alkaline condition, bp-VMSF stably grows, the surface of the inner layer close to the p-GCE electrode is negatively charged, and the surface of the outer layer away from the electrode is positively charged. The asymmetric charge configuration enhances the adsorption capacity of positively charged adriamycin molecules on an electrode interface through electrostatic enrichment. The p-GCE adsorbs adriamycin and promotes electron transfer, and is combined with the synergistic enrichment effect of the bp-VMSF double-static nano-channel, so that the electrochemical response performance to adriamycin is jointly improved, a method is provided for rapid detection of the liposome adriamycin encapsulation efficiency, and the p-GCE has a good prospect in evaluation of liposome adriamycin preparations.

The invention provides a method for detecting chloramphenicol residues in animal food by adopting a molecular imprinting sensor, which comprises the following steps: preparing the molecular imprinting sensor with specific recognition on CAP, and providing an electrochemical detection system of the molecular imprinting sensor for detecting the chloramphenicol residues in the animal food, fitting to obtain a calculation formula of chloramphenicol concentration detected by the molecularly imprinted sensor; treating an animal food sample, and mixing the animal food sample with a standard concentration CAP solution according to a volume ratio of 1: 9 to obtain a to-be-detected sample solution; scanning the to-be-detected sample liquid through the molecular imprinting sensor by adopting a square wave voltammetry method to obtain a peak current value; and obtaining the concentration of chloramphenicol in the sample solution to be detected according to the peak current value and a calculation formula of chloramphenicol concentration detected by the molecular imprinting sensor. The method is sensitive, accurate and practical.

The invention discloses a high-throughput electrochemical detection platform based on an ultra-infiltration micro-droplet array, a preparation method of the high-throughput electrochemical detection platform and application of the high-throughput electrochemical detection platform in AD marker detection, and belongs to the technical field of intersection of analytical chemistry, biosensing and material science. The method comprises the following steps: cleaning and drying a substrate, and immersing the surface of the substrate in a fluorosilane solution to form a super-hydrophobic layer by adopting a dip-coating method; after covering with a mask plate, etching with O2 plasma so as to form a hydrophilic microdot matrix; respectively printing an Ag / AgCl reference electrode, a gold working electrode and a carbon counter electrode on the hydrophilic microdots; electrode wires are printed layer by layer, a reference electrode wire, a working electrode wire and a counter electrode wire are sequentially printed in the area outside the hydrophilic microdot matrix from bottom to top in a spin-coating PDMS layer separation mode after each layer of electrode wire is printed, and PDMS is spin-coated above the counter electrode wire for packaging after printing is completed; and on each gold working electrode, respectively modifying capture antibodies of A beta 42, A beta 40, P-tau181 and P-tau217 proteins through a sample application instrument, so as to obtain the detection kit.

The invention provides construction of an electrochemical biosensor based on a fucoidin hydrogel microneedle and application of the electrochemical biosensor in online monitoring of uric acid, and relates to the technical field of biosensors. According to the micro-needle, Gantrez S-97 and PEG are subjected to copolymerization crosslinking to form a hydrogel main body, fucoidin is introduced for blending modification, a three-dimensional network structure is constructed by utilizing a high-temperature esterification reaction for regulating and controlling pH, and the super-expansion hydrogel micro-needle with high swelling property and good biocompatibility is formed. The microneedle can rapidly collect a skin interstitial fluid sample, and then through a screen printing electrode (SPE) platform, carboxylated MWCNTs are used for modifying an electrode and immobilizing uricase to construct an enzyme sensor. Hydrogen peroxide generated by catalyzing uric acid on the surface of the electrode participates in electrochemical reaction, and an output signal is used for real-time detection of uric acid concentration. The device integrates minimally invasive sampling and high-sensitivity electrochemical detection, has the advantages of quick response, simplicity and convenience in operation, good biocompatibility and the like, and is suitable for application scenes of continuous monitoring of uric acid, chronic disease management and the like.

The invention discloses an AI-enabled real-time electrochemical detection system, which is composed of a real-time electrochemical detection module and an embedded AI data processing and analysis module, the real-time electrochemical detection module comprises an electrochemical electrode system, a sample pool and an electrochemical workstation, the surface of a working electrode is modified with a probe of a specific recognition target, and when the probe is combined with the target, the probe of the specific recognition target can be detected by the embedded AI data processing and analysis module. The working electrode interface charge transfer impedance changes in real time to generate a dynamic electric signal, and the electrochemical workstation provides electric input and collects the signal; the embedded AI data processing and analysis module is used for denoising the dynamic electric signals through an adaptive filtering algorithm based on wavelet transform, extracting time-frequency domain joint features through a 1D-CNN and LSTM mixed deep learning model, and establishing a nonlinear mapping relation between the signals and target concentration. Through AI enabling electrochemical detection, the defects of low time efficiency, large error and low information utilization rate of the traditional technology are comprehensively overcome, and an innovative solution is provided for high-precision, rapid and anti-interference target substance detection.

The utility model discloses an electrochemical reaction tank for a hard X-ray absorption spectrum in-situ dual-mode test. The electrochemical reaction tank comprises a reaction tank body, a working electrode, a reference electrode and a graphite counter electrode, the front plate is provided with a working electrode window, and the upper plate is provided with a reference electrode hole and a graphite counter electrode hole. The working electrode is arranged at the window of the front plate and is tightly attached to the reaction tank body through a polyimide adhesive tape, and the reference electrode and the graphite counter electrode are arranged on the two sides of the reaction tank body and are respectively connected with an external electrochemical working platform. The distance between the working electrode window and the rear plate is controlled, and the working electrode is coupled with synchrotron radiation X-ray incident light, a fluorescence signal and a transmission signal to form an X-ray dual-mode test channel. The advantages of a synchrotron radiation X-ray absorption spectrum measuring platform are fully utilized, a real in-situ electrochemical detection environment is simulated, and reliable fluorescence and transmission signals are collected at the same time.

A fuel cell control system, which includes an electrochemical detection apparatus, a control apparatus and a power converter, and is used for coupling a fuel cell to supply power to a load. The electrochemical detection apparatus includes a sensing apparatus and a processor. The sensing apparatus is used for sensing a cell current and a cell voltage. The processor is used for executing electrochemical analysis on the basis of the cell voltage and the cell current, and determining a water content state of the fuel cell. The control apparatus is used for adjusting a fuel state of the fuel cell when the water content state is a dry film state or a water-saturated state. The power converter is used for determining a load condition of the fuel cell, and generating output power on the basis of the cell voltage and the cell current when the water content state is in a normal state, wherein the cell voltage and the cell current are related to the load condition. The present disclosure further relates to a fuel cell control method and an electrochemical detection apparatus.

This invention discloses an Au / CeO2-BTC nanozyme based on strong metal-support interactions and its application in electrochemical glucose detection. The nanozyme uses a Ce-BTC metal-organic framework as a precursor, which is calcined to obtain a porous CeO2-BTC support rich in oxygen vacancies. Gold nanoparticles are then loaded onto the support via in-situ deposition. The product forms Au-O-Ce interfacial active sites and exhibits significant strong metal-support interactions, enabling electronic regulation and enhancing CeO2-support electrochemical detection. 3+ The content is increased and the charge transfer rate is accelerated. The electrode modified with this Au / CeO2-BTC nanozyme exhibits excellent catalytic performance for glucose oxidation, with a sensitivity of 41.3 μA·cm. ‑2 ·mM ‑1 The detection limit is as low as 4.75 μM, and it exhibits good reproducibility, stability, and selectivity.

The invention discloses a monatomic / nitrogen-doped carbon sphere material and a preparation method and application thereof, and relates to the technical field of electrochemical detection.The preparation method comprises the following steps that glucose and hexadecyl trimethyl ammonium bromide are added into deionized water to be subjected to a hydrothermal reaction, and carbon spheres are obtained; mixing, grinding and calcining the carbon spheres and urea to obtain nitrogen-doped carbon spheres; and adding a metal salt solution into the nitrogen-doped carbon sphere suspension, stirring, filtering, washing, drying, and finally calcining in a nitrogen atmosphere to obtain the monatomic / nitrogen-doped carbon sphere material. The invention also discloses the monatomic / nitrogen-doped carbon sphere material prepared by the method and application of the monatomic / nitrogen-doped carbon sphere material in electrochemical detection of organic pesticides. According to the nitrogen-doped carbon sphere, the single metal atoms are anchored on the vacancies on the nitrogen-doped carbon sphere, so that the sensitivity, the detection limit, the selectivity, the stability and the anti-interference capability of the nitrogen-doped carbon sphere when the nitrogen-doped carbon sphere is used for electrochemical detection of organic pesticides are remarkably optimized, and the nitrogen-doped carbon sphere has important practical application value.

The invention provides an S100B and NSE double-signal electrochemical immunosensor, a preparation method, application and a detection product, and belongs to the technical field of electrochemical detection. According to the preparation method, the PAMAM dendritic polymer rich in amine-terminated functional groups is covalently grafted to the MXene conductive sheet layer with a large surface area to prepare the PAMAM-MXene composite material, so that the immobilization efficiency and interface electron transfer of immune molecules are remarkably improved through modification, and enhanced conductivity and relatively high specific surface area are shown; and the biomolecule loading capacity and the signal transmission efficiency of the electrode are also remarkably improved, and double-signal detection can be performed on S100B and NSE at the same time. And the electrochemical immunosensor has good reproducibility, long-term stability and anti-interference capability on the two biomarkers. According to the sensor, modular design and signal decoupling are adopted, double targets can be accurately detected in parallel, and an extensible strategy is provided for multiplexing biomarker analysis.

The invention relates to an aptamer electrochemical biosensing system based on a support vector machine. The aptamer electrochemical biosensing system is used for detecting the content of CEA in body fluid. The system comprises an electrochemical detection module and a signal processing module for identifying and correcting a signal value of the electrochemical detection module, the electrochemical detection module is used for detecting and acquiring an electrochemical signal based on a working electrode with CEA aptamer DNA (Deoxyribose Nucleic Acid); the signal processing module comprises an acquisition unit for collecting the electrochemical signals, a feature extraction unit for extracting electrochemical features of the electrochemical signals, a model judgment unit for judging and processing the electrochemical signals, and a signal output unit for outputting final electrochemical signals; the model judgment unit is based on a support vector machine algorithm. According to the system, the detection stability and the pH anti-interference capability can be remarkably improved, high-precision concentration judgment can be realized, and the system has intelligent compensation and engineering practicability.

The invention provides an innovative dual-mode detection method based on a dipeptide nano framework (DNPs), which is used for efficiently detecting helicobacter pylori. According to the method, firstly, a DNPs material formed by chelating dipeptide (Trp-Phe) and zinc ions (Zn (II)) is synthesized, and the material is decomposed along with the increase of the pH value in an alkaline environment, so that the Zn (II) is released; then, urea is hydrolyzed under the catalysis of urease to generate ammonia, so that the pH value of the solution is increased, and the decomposition of DNPs and the release of Zn (II) are further promoted. By utilizing an electrochemical detection mode, real-time analysis on urea and urease is realized by monitoring an electrochemical signal of Zn (II); meanwhile, in combination with the fluorescence characteristics of DNPs, the concentration of urea and urease is indirectly and quantitatively analyzed by monitoring the fluorescence intensity change. The dual-mode detection method combines the advantages of electrochemistry and fluorescence detection, has high sensitivity and real-time performance, provides an effective means for accurate detection of helicobacter pylori, and shows a wide application prospect in clinical diagnosis and sensing technologies.

This invention discloses a G4-DNA tetrahedral probe electrode, its preparation method, applications, and application methods. This invention utilizes four DNA strands to self-assemble into a DNA tetrahedral structure based on Watson-Crick hybridization. The DNA tetrahedron and G4 strand can be connected to the electrode based on the interaction between thiol groups and the gold electrode, and complementary base pairing. The addition of the DNA tetrahedron, through the construction of a reasonable probe density, further improves the detection sensitivity. This invention utilizes square wave voltammetry for electrochemical detection of malachite green. The content of malachite green can be determined based on the strength of the current signal of its characteristic peak. Rapid detection of malachite green can be achieved in a short time, with a minimum detection concentration of 3.65 μg / kg, showing potential for on-site detection. Six repeated tests using this electrode yielded almost identical detection signals, indicating that the electrode has good reusability and stability.

The invention discloses a dual-mode detection kit for highly sensitive detection of ERalpha based on HRP-streptavidin. The dual-mode detection kit comprises the following raw materials: a glassy carbon electrode, 1-AP-CNHs, AuNPs, Sh-Apt, MCH, Bio-Ab, and HRP-streptavidin. The invention successfully constructs an electrochemical detection kit for detecting ERalpha with high sensitivity on the basis of the 1-AP-CNHs / AuNPs composite material. According to the invention, due to the redox activity of HRP-streptavidin, the HRP-streptavidin and H2O2 are subjected to redox reaction to generate a current signal; in addition, hydrogen peroxide can be catalyzed to generate. OH free radicals, and the free radicals can oxidize TMB to generate blue products, so that colorimetric reaction is initiated, and ERalpha can be detected more accurately. The electrochemical-colorimetric dual mode of the 3D 1-AP-CNHs / AuNPs composite material and the HRP-streptavidin for high-sensitivity detection of the ERalpha disclosed by the invention greatly improves the detection accuracy. The kit has the advantages of good selectivity, strong stability, high sensitivity, anti-interference performance, environmental protection and the like.

The application discloses a four-electrode voltammetry oil product acid-base value detection circuit design method, and belongs to the technical field of electrochemical detection. The method comprises the following steps: designing a four-electrode system, a controllable current source, a voltage sensing unit and a regulation circuit to form a complete oil product acid-base value detection circuit. The four-electrode system comprises an auxiliary electrode, a working electrode and two reference electrodes; the controllable current source outputs an excitation current and injects the excitation current into a detection solution through the auxiliary electrode and the working electrode; the voltage sensing unit collects a response potential difference of the detection solution through the two reference electrodes; the regulation circuit adjusts the output excitation current of the controllable current source in a current scanning or voltage scanning mode and synchronously collects the response potential difference, constructs a voltammetry curve between the excitation current and the response potential difference, and calculates a reaction peak area parameter and a corresponding acid-base value of the measured oil product. The application can overcome the problem caused by the working electrode potential drift of the traditional three-electrode type oil product acid-base value detection circuit, and thus stable and accurate detection results are obtained.

The application provides a preparation method and application of a flexible electrochemical sensor and belongs to the technical field of electrochemical detection. The preparation method is as follows: first, polyurethane sponge is soaked in a conductive carbon paste dispersion solution to obtain a conductive support; second, the conductive support is soaked in an aqueous chloroauric acid solution for electrochemical deposition to obtain a gold nano-structure layer modified conductive support; third, the gold nano-structure layer modified conductive support is placed in a groove of a PDMS base, and the gold nano-structure layer modified conductive support is separated into a working area and a clamping area by pouring PDMS to obtain a working electrode; and finally, a flexible electrochemical sensor is assembled. The flexible electrochemical sensor is used for hydrogen peroxide detection and has good biocompatibility, excellent tensile properties and stable electrochemical sensing performance.

This invention discloses a functionalized biomodified electrode for rapid pathogen detection and its preparation method, relating to the fields of biosensing and electrochemical detection technology. It includes a glassy carbon electrode substrate, the surface of which is sequentially modified with a reduced graphene oxide layer, an electropolymerized polydopamine film, and an electrodeposited gold nanoparticle layer, forming a composite nanomaterial modified electrode. Antibodies are captured and fixed to the electrode surface through covalent interactions. This functionalized biomodified electrode for rapid pathogen detection and its preparation method, using the same electrochemical workstation, first electropolymerizes dopamine to form a film and then electrodeposits gold nanoparticles. This allows for precise control of film thickness, gold particle size, and coverage density by parameters such as polymerization potential, scan cycles, deposition potential, and deposition time. This avoids batch-to-batch fluctuations caused by dissolved oxygen and solution disturbances in traditional chemical methods, reducing the relative standard deviation of peak current detection from different batches of electrodes from 18.7% to 4.2%–4.5%.

The utility model discloses a portable glucose electrochemical detection device which comprises a device main body, a limiting mechanism is arranged in the device main body, a baffle block is arranged on one side of the surface of the device main body, and a locking mechanism is arranged on one side of the baffle block; a cover plate is matched with a cover plate groove, an auxiliary sliding block slides along the cover plate groove, the placement groove is quickly opened, a detection instrument is placed in the placement groove, and the effect that a threaded rod is adjusted to slide so as to adapt to and position the detection instrument is achieved in cooperation with rotation between gears; by pulling the bottom cover to be matched with the test port cover plate and the limiting rod to move, the purposes of positioning the spring bulge and facilitating the use of the test port are achieved, and the bottom cover is enabled to seal the device by matching with the reset spring and utilizing the rapid springback reset capability of the reset spring.

The application discloses a preparation method of an Fe-NC@CNFs / GCE electrode and application thereof, relates to the technical field of electrochemical detection, and has the technical scheme as follows: a bimetallic ZIF based on Fe / Zn is synthesized, and is calcined at a high temperature of 800 DEG C to obtain a derived nitrogen-doped porous carbon material (Fe-NC@CNFs); the synthesized material has a large specific surface area and rich active sites; the material is configured into a suspension by using ultrapure water; and a traditional drop-coating method is adopted to successfully build an electrode Fe-NC@CNFs / GCE for detecting luteolin; the electrode has high selectivity, sensitivity and stability, and can detect luteolin in a natural sample.