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Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro ... Bead-immobilized DNA templates would provide a convenient way to circumvent these problems by enabling the bead-bound DNA templates to be collected and recycled after each transcription reaction. ... permits the synthesis of large proteins not ...
Continuous in-vitro transcription system
PCT designated stageWO2026072640A1Heating or cooling apparatusMicrobiological testing/measurementImmobilized DNACell biology
The single use system for continuous in-vitro transcription of RNA biomolecules from an immobilized DNA template includes a pair of recirculation loops, each recirculation loop powered by at least one low shear pump controlled by a singular use back pressure control valve. The single use system for continuous in-vitro transcription of RNA biomolecules from an immobilized DNA template further includes a single-use sensor controlling flow and the pressure within each recirculation loop. The single use system for continuous in-vitro transcription of RNA biomolecules from an immobilized DNA template includes a bypass valve opening on each recirculation loop permitting a stream from each loop releases to join in a residence spool, a mixture will preheat to a target temperature of a reactor.
Owner:N 1 PROCESS ENGINEERING SOLUTIONS LLC