427 results about "Specific protein" patented technology

The present invention relates to various molecular constructs having at least one cellular-targeting element, one protein-targeting element, and one ubiquitin ligase recruitment element, wherein these elements are conjugated in a manner that facilitates cellular uptake and targeted degradation of specific proteins within cells. The present invention further relates to multifunctional proteolysis-targeting chimera conjugates and uses thereof for the treatment of diseases or disorders such as immune and inflammatory diseases, infectious diseases, cardiovascular diseases, endocrine disorders, and various cancers that are otherwise resistant to traditional therapeutic regimens.

The invention provides a composition with oil-controlling, acne-removing, barrier-repairing effects and application thereof, and belongs to the field of cosmetics. The composition is prepared from a baropetalum beetle leaf extract, a roselle flower extract, totarol and a cryptomeria fortunei bud extract. The baropetalum chinense leaf extract has the effects of balancing grease secretion and shrinking pores; the roselle flower extract is rich in organic acid and anthocyanin, and has the effects of dissolving pore keratosuppository, controlling oil, resisting inflammation, smoothing acne skin, preserving moisture and removing redness; as a bacteriostatic agent, totarol has a natural preservative effect on gram-positive bacteria and partial negative bacteria, and has the effects of removing acnes, sterilizing, diminishing inflammation, whitening and the like; the cryptomeria fortunei bud extract can promote skin tissue structure regeneration and strengthen skin barriers. The composition effectively regulates and controls excessive secretion of sebaceous gland grease and pox skin specific protein through the synergistic effect of various components, promotes the health of pox skin, repairs the skin barrier and enhances the self-protection ability of the skin at the same time.

The invention belongs to the field of functional proteomics research, and particularly relates to a combined analysis method for analyzing interaction between a small molecule substance and a targeted protein, which is used for analyzing the small molecule substance and the protein by adopting LiP-MS (Limited Protein-Mass Spectrometry) technology and phosphorylated proteome combined analysis. According to the method, the optimized LiP-MS technology is adopted, the target protein can be directly recognized under the complex proteome background without enriching specific protein, and the problem that low-abundance protein is difficult to detect is solved; phosphorylated proteome abundance transformation protein is analyzed by combining high-resolution mass spectrometry, so that the recognition capability on a drug target and a shear variant thereof is enhanced, and the detection sensitivity is improved.

The present disclosure relates to compounds of formula (I) and their anti-tumor uses, and their intermediates of formula (III), intermediates of formula (IV), and uses of the intermediates. The compound of formula (I) has a degrading effect on a specific target protein, which is mainly composed of three parts. The first part is a small molecule compound (SMBP, Small Molecules Binding Protein) that can bind to a protein, the second part LIN is a linker, and the three-part ULM is a ubiquitin ligand (ULM, Ubiquitin Ligase Binding Moiety), wherein SMBP is covalently bound to LIN, and LIN is covalently bound to ULM. A series of compounds designed and synthesized in the present disclosure have a wide range of pharmacological activities, including the functions of degrading specific proteins and / or inhibiting activities of specific proteins, and thus can be used in related tumor treatments.

The invention relates to the technical field of bioactive peptides, in particular to a novel multifunctional collagen-like tripeptide and application thereof, an amino acid sequence contains multiple combinations, X can be selected from multiple components, 3-methoxy-4-hydroxyphenylalanine and the like are added, and the novel multifunctional collagen-like tripeptide can interact with specific proteins, activate related pathways and play multiple physiological functions and is applied to multiple fields. The collagen-like tripeptide has obvious advantages, innovates component expansion functions, improves the preparation method, improves the efficiency, reduces the cost, is matched with other components in the fields of beauty, health care and medicine, enhances the efficacy, realizes targeted delivery, and brings a new opportunity to related industries.

The invention discloses a composition for treating diseases through protein degradation in a novel degradation way, and relates to the technical field of medicines. Comprising a mandolin-based androgen receptor degradation chimeric peptide (MbTACs), the mandolin-based androgen receptor degradation chimeric peptide (MbTACs) contains a targeting module YLCSSNNRERDKFRR combined with AR and a module combined with midnolin, a KLVFF sequence and a cell penetrating peptide PKKKPKV are added to the C end of the peptide, and a polypeptide molecule MbAD is formed. According to the invention, a midnolin-based MbTACs platform is developed, and the midnolin-based MbTACs platform is used for degrading specific proteins. The bifunctional molecules form a midnolin-MbTACs-target protein ternary complex between the target protein and the midnolin, and then the protein is degraded through a midnolin-proteasome way. The MbTACs can effectively degrade c-Myc and androgen antibodies in vitro and in vivo, and play an anti-tumor role. The MbTACs molecule represents a first midnolin-based target chimera, and the MbTACs molecule represents a first midnolin-based target chimera.

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention belongs to the technical field of medical detection, and provides an automatic specific protein analyzer for comprehensive multi-item detection, which mainly comprises an online conveying track, an urgent treatment assembly, a double-sample-arm sampling mechanism, a sample disc, a reagent disc, a reaction disc, an ISE electrolyte module and a double-light-path detection module. The online conveying track is used for automatic detection and transmission of samples and online connection of adjacent detection instruments, the urgent treatment assembly is used for urgent detection of the samples, the double-sample-arm sampling mechanism is used for selective sampling of different samples, the ISE electrolyte module is used for electrolyte detection, and the double-sample-arm sampling mechanism is matched to achieve electrolyte module detection. The detection sequence and speed of other samples are not delayed; the double-light-path detection module is used for detecting different samples, the photosensitive sensitivity is improved, and the detection efficiency is improved. By adopting the structure, the detection requirements and sampling conditions of a current detection instrument on different samples are met, and the compatibility and detection efficiency of the detection instrument are improved.

The invention provides a tumor cell vaccine as well as a preparation method and application thereof. The preparation method comprises the following steps: acquiring and culturing tumor cells; enabling the cell membrane of the tumor cell to express a targeting antibody by utilizing a gene vector infection or gene editing technology, and enabling the targeting antibody to be used for enabling the tumor vaccine to be combined with the dendritic cell; performing overexpression of tumor specific protein on the cell membrane surface of the tumor cell by using a gene vector infection or gene editing technology; a nucleic acid substance of a target protein is wrapped by lipid nanoparticles to form a vaccine framework, and the nucleic acid substance can enable target cells to express directional chemotactic molecules; wherein the directed chemotactic molecule is used for transferring the dendritic cells to lymph nodes; the target vaccine is obtained by wrapping the vaccine framework with the cell membranes of the tumor cells, lymph node homing of the dendritic cells can be promoted, and the antigen presentation efficiency is increased and the immune effect is enhanced through tumor specific protein carried by the target vaccine and assisted by an immunologic adjuvant.

The present disclosure presents a general approach to engineering existing protein-protein interactions through domain addition and evolution. The disclosure teaches the creation of novel fusion proteins that include knottin peptides where a portion of the knottin peptide is replaced with a sequence that has been created for binding to a particular target. Such fusion proteins can also be bispecific or multi specific in that they can bind to and / or inhibit two or more receptors or receptor ligands. Knottins may be fused with an existing ligand (or receptor) as a general platform tor increasing the affinity of a ligand-receptor interaction or for creating a multi specific protein. In addition, the fusion proteins may comprise a knottin peptide fused to another protein where the other protein facilitates proper expression and folding of the knottin.

The invention belongs to the technical field of exosome modification, and particularly relates to an exosome modification method for connecting a target protein to the surface of an exosome by utilizing NHS-PEG-NHS to achieve a targeting or treatment effect, and application of the exosome modification method in targeted central nervous system demyelination disease treatment. In particular to a technology for modifying an exosome to enable the exosome to have targeting property and a preparation method of the exosome. NHS-PEG-NHS is used as a connecting substance, and protein, polypeptide and substances with primary amine groups on the surface can be connected to the surface of an exosome membrane. The modified exosome can load drugs and can be accurately delivered to tissues expressing specific proteins or polypeptides through oral administration, so that the bioavailability and the treatment effect of the drugs are remarkably improved. Taking the NG2 antibody modified cow milk exosome as an example, the NG2 antibody is connected to the surface of the cow milk exosome to target the central demyelination focus area, so that the effective therapeutic dose of the drug in the central focus area is achieved through oral delivery.

The invention discloses a single nucleotide mutation site SNP (Single Nucleotide Polymorphism) and KASP (Kinase Associated Specific Protein) marker remarkably associated with soybean plant height and application thereof. The SNP molecular marker is located at the 36108741bp position of the fifth chromosome of the soybean, the basic group is T or C, the SNP molecular marker is significantly related to the plant height phenotype of the soybean, and the plant height of the soybean variety with the locus genotype of TT is significantly lower than that of the soybean variety with the genotype of CC; three KASP primers are developed according to the SNP site and are respectively SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, PCR amplification and genetic typing are carried out on to-be-detected soybeans by utilizing the primers, and if a detection result shows that the basic group type at the marker position is T, it is judged that the plant height of the soybean variety is relatively low; if the detection result is C, it is judged that the plant height is high. The SNP molecular marker disclosed by the invention can be used as an auxiliary selection marker for the plant height character in the soybean breeding process, so that the selection accuracy is improved, and the soybean plant height character related breeding process is accelerated.

The invention relates to a UGT enzyme subtype substrate specificity prediction method and model. On the basis of a directional message passing neural network, graph structure characterization of a small molecule compound and features of specific protein binding sites of UGT enzyme are deeply fused, a bimodal prediction normal form of'molecule + protein binding sites' is designed, a deep learning model is constructed, conversion from compound center prediction to molecule-enzyme binding site comprehensive prediction is achieved, and the prediction accuracy is improved. And accurate classification prediction can be carried out on UGT enzyme substrates and non-substrates.

Provided are proteins capable of engaging NK cells in cancer therapy, especially multi-specific proteins capable of binding IL 15 receptor, NKp46, target antigen of interest, and optionally Fc gamma receptor such as CD16a. Also provided are NKp46-binding proteins. The proteins have utility in the treatment of diseases, such as cancer.

The invention relates to a urine specific protein detection sample quality screening system and screening method. The screening method comprises the following steps: firstly, creating a urine specific protein quality model; then collecting an image of a to-be-detected sample; and finally, analyzing and evaluating the sample to be detected according to the sample quality model and the image of the sample to be detected, and converting urine colors (such as light yellow, deep yellow, hematuria and the like) into quantifiable numerical values by collecting the image of the sample (urine). Compared with traditional naked eye observation, judgment deviation caused by subjective factors such as experience and ambient light of detection personnel is avoided, and the detection result is more objective and repeatable; according to the method, the sample image is converted into quantifiable numerical values so as to carry out numerical analysis on the detection data of the color, clarity and turbidity of the sample, hematuria and urine with high turbidity in the sample are automatically screened out, manual operation steps are reduced, the detection efficiency is improved, and the method is particularly suitable for clinical or large-scale screening scenes.

The utility model discloses a novel protein marker diagnostic kit for detecting the muscle injury degree, which comprises a detection kit for detecting the muscle injury degree and a dustproof cover assembled above the detection kit, the detection kit is provided with a detection port for dropping blood of a patient and a display port for displaying a diagnosis result, a reagent card for evaluating the muscle injury degree by detecting the concentration of a specific protein marker in the blood is arranged in the detection kit, and the detection end of the reagent card is located below the detection port. The display end of the reagent card is positioned below the display opening; a positioning opening for positioning the dustproof cover is formed above the detection kit, and a positioning block matched with the positioning opening is arranged in the dustproof cover. According to the utility model, the muscle injury degree of a patient can be detected by quickly reading the novel protein marker in the blood, so that an accurate diagnosis and treatment basis is provided for the treatment of the patient, the detection time of the patient is shortened, and the treatment cost is reduced.

The invention provides a recombinant exosome as well as a preparation method and application thereof. The preparation method of the recombinant exosome comprises the following steps: transfecting a target cell with an exosome loaded with nucleic acid molecules, and culturing to prepare the recombinant exosome; the nucleic acid molecule sequentially comprises a promoter sequence, a sequence for coding beta-globin, a 5 '-UTR, a target gene sequence, a sequence for coding alpha-globin, a 3'-UTR and a terminator sequence from the 5'end to the 3 'end. The method not only can be used for producing the recombinant exosome of the specific protein, but also has high flexibility and controllability. The recombinant exosome produced by the preparation method disclosed by the invention shows good stability and biological activity in cell culture and in-vivo experiments, and therapeutic protein can be effectively delivered to target cells. According to the technology, a virus-free and non-integrated treatment method is provided, and common risks in traditional gene therapy are reduced.

The invention discloses a multifunctional tumor targeted low-temperature treatment nano platform and application thereof, and belongs to the technical field of nano biological medicines. According to the multifunctional tumor targeted low-temperature treatment nano platform, the two-dimensional Ti3C2 nano material serves as a carrier, indocyanine green is loaded to serve as a photosensitizer, polydopamine is modified on the surface to improve the stability, and gambogic acid is loaded to serve as an anti-tumor drug and a heat shock protein inhibitor; and a nucleic acid aptamer capable of recognizing specific protein on the cell surface is coupled in a covalent binding manner. The multifunctional tumor targeted low-temperature treatment nano platform is small in particle size, high in dispersion degree and good in biocompatibility, and shows excellent photo-thermal conversion capacity, photodynamic treatment effect and chemotherapy effect in tumor targeted treatment. The apoptosis of tumor cells can be effectively induced under a relatively low temperature condition, and the damage of high temperature to surrounding healthy tissues is remarkably reduced. The tumor focus specific recognition capability is also realized, the high enrichment at the tumor part can be realized, and the treatment effect is enhanced.

The invention is applicable to the technical field of vaccines, and provides a haemaphysalis unguiculata salivary gland multi-component subunit vaccine as well as a preparation method and application thereof, the effective components of the vaccine comprise rHlPDI-2 and rHlPrx soluble recombinant proteins, and the amino acid sequences of the rHlPDI-2 and rHlPrx soluble recombinant proteins are shown as SEQ ID NO.1-2. Starting from salivary gland secretory protein related to tick activity in a host biting process of haemaphysalis unguiculata, special protein of salivary glands of tick species is researched, the recombinant protein vaccine is prepared, survival and reproduction of haemaphysalis unguiculata can be affected, and the prepared multi-component subunit vaccine has an interference effect on biting and passage of haemaphysalis unguiculata and can be used for preparing the multi-component subunit vaccine for preventing and treating haemaphysalis unguiculata. The anti-tick vaccine has a good anti-tick effect, contributes to prevention and treatment of tick-borne diseases, and lays a foundation for development of other tick related vaccines.

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

The invention relates to the technical field of sample analysis, and discloses a specific protein detection method based on double-angle scattering turbidimetry, which comprises the following steps: S1, obtaining a small-angle signal concentration relation curve and a medium-angle signal concentration relation curve, and obtaining a cross threshold signal; s2, the laser light source irradiates the reaction cup from one side of the reaction cup along the horizontal axis, the detection module is located on the other side of the reaction cup and opposite to the laser light source, and the detection module collects small-angle and medium-angle scattering signals at the same time and transmits collected scattering signal information to the control module; and S3, the control module compares the received medium-angle scattering signal information with the cross threshold signal information, dynamically selects and adopts a small-angle scattering signal, and obtains the concentration of the specific protein to be detected according to a small-angle signal concentration relation formula. Or obtaining the concentration of the specific protein to be detected by adopting the medium-angle scattering signal according to a medium-angle signal concentration relation formula. The method can improve the accuracy of protein concentration analysis.

The invention discloses an active tuberculosis diagnosis method based on proteomics and metabonomics. The method comprises the following steps: collecting a serum sample of a suspected active tuberculosis patient; detecting specific proteins in the serum sample by using a proteomics technology; detecting specific amino acids in the serum sample by using a metabonomics technology; carrying out standardization treatment on the obtained protein, amino acid and bile acid data; establishing a diagnosis model by using a machine learning algorithm; inputting data of a to-be-diagnosed sample into the model, and calculating a probability value of the to-be-diagnosed sample suffering from active tuberculosis; according to a preset threshold value, whether the sample provider suffers from the active tuberculosis or not is judged. By comprehensively analyzing multiple omics data, the sensitivity and specificity of diagnosis are greatly improved; by utilizing a machine learning algorithm, the disease risk can be objectively and quantitatively evaluated, and human misjudgment is reduced; the detection process is simple and fast, the sample demand is small, and clinical popularization and application are facilitated.

Provided herein are methods of treating or preventing the formation of orthopedic-related fibrosis, which included but not limited to peri-implant fibrotic tissue, bone-to-bone fibrosis, bone-to-soft tissue fibrosis, soft tissue-to-soft tissue fibrosis, joint related fibrosis, in a subject in a subject in need thereof. In certain embodiments, the method includes administering to the subject an inhibitor of ADGRF5, GREM1, PTPRF, LAMB1, EFEMP1, DNMT3b, RSPO3, ACTA2, PDGFRA, PDGFB, PDGFA, CX3CR1, TBX18, PERI, NR1D1, RYR1, DES, KLF4, KLHL41, DUSP1, or TNNT3 to the subject.

The present disclosure encompasses systems, methods, and compositions for diagnosing a subject for a high-grade advanced pre-malignant lesions or colorectal cancer (CRC) disease state by ascertaining the presence of certain one or more glycosylated or aglycosylated peptides in liquid biopsy samples from the subject. Specific embodiments encompass methods of measuring certain one or more glycosylated or aglycosylated peptides in liquid biopsy samples from subjects known to have or suspected of having a high-grade advanced pre-malignant lesions or CRC disease state or subjects undergoing routine health care maintenance for possible presence of a high-grade advanced pre-malignant lesions or CRC disease state. The disclosure provides systems, methods, and compositions to identify subjects at-risk for CRC or high-grade advanced pre-malignant lesions and increases subject colonoscopy compliance, in specific embodiments.

The invention relates to a method for separating out and condensing specific protein in liquid for dyeing and detection. The liquids include but are not limited to human samples, human blood, saliva, urine and the like. The liquid is diluted and its pH is raised or lowered to form a mixed liquid. Placing the mixed solution in an electrostatic field, moving the specific protein in the mixed solution to one side of the mixed solution, accurately dropwise adding an acidic or alkaline solution, adjusting the pH value of the mixed solution, enabling the pH value of the mixed solution to reach the isoelectric point of the specific protein, and enabling the specific protein to be separated out and precipitated on one side of the mixed solution. In addition, according to the sequence of the pH values from large to small or from small to large, the isoelectric points of all the specific proteins can be traversed in the process, and all the specific proteins can be separated out and precipitated in the same area of the mixed solution.

The invention provides a method for screening and verifying kelp chloroplast prosthesis protein ACE (angiotensin converting enzyme) inhibitory peptide, which comprises the following two steps of: 1, carrying out computer-assisted enzymolysis on kelp protein, selecting a representative protein sequence in kelp by using an online database, selecting specific protease by using an online enzymolysis platform to carry out virtual enzymolysis, and screening and verifying the ACE inhibitory peptide according to an enzymolysis result; establishing a kelp protein polypeptide classification database; and step 2, establishing a QSAR model of the ACE inhibitory active peptide, and predicting the activity of the ACE inhibitory active peptide, so that the invention provides a research and development method system for a functional product rich in the ACE inhibitory active peptide based on the combination of kelp bioinformatics and experimental verification.

The invention relates to the technical field of bioinformatics and molecular cytogenetics, in particular to a method for counting centromeres of chromosomes based on a Nuf2 antibody and application. The method comprises the following steps: performing chromosome centromere counting on a Nuf2 antibody and a centromere specific protein Nuf2 through an immunofluorescence reaction, so that the number of chromosome centromeres can be directly observed, and the number of chromosomes can be efficiently and quickly determined. Besides, due to the fact that the specific protein Nuf2 of the centromere is highly conservative in eukaryotes, the invention further provides application of the method to identification of the plant ploidy, the method is easy and convenient to operate and low in cost, the ploidy identification efficiency is remarkably improved, and a universal technical platform is provided for genetic breeding.

The disclosed systems and methods involve the semi-quantitative detection of disease-specific protein signatures in biological samples. The system may include a housing with a motor driven substrate holder on rails. The substrate holder is configured to be positioned at multiple locations along the guide rails. Imaging sensors capture images of portions of the substrate at these locations. The system detects markers on the substrate and determines the location of wells based on these markers. Based on the determined location of the wells, color values for each pixel in the wells can be measured. These values may be analyzed to detect disease-specific protein signatures in a variety of sample types.

The present invention relates to a method for transforming a strain of a lactic acid bacterium, such as of the Lactococcus genus through competence for natural transformation. The present invention further relates to strains obtained or obtainable by this method. The present invention also relates to the use of specific strains of a lactic acid bacterium, such as of the genus Lactococcus having inherently low, reduced or disrupted activity of specific proteins for transforming these strains through natural transformation. The present invention further relates to methods for increasing the rate of natural transformation by increasing competence for natural transformation of a strain of a lactic acid bacterium, such as of the genus Lactococcus and for identifying a strain of a lactic acid bacterium, such as of the Lactococcus genus which is transformable through competence for natural transformation.

The invention relates to the technical field of medical instruments, in particular to a specific protein analysis system. The system comprises a sample management device, a sample conveying device and a protein analysis device, the sample management device is used for storing a plurality of sample racks and conveying a target sample rack to a waiting station; the sample conveying device is used for conveying the target sample frame at the waiting station to the sampling station and conveying the target sample frame at the sampling station back to the waiting station; and the protein analysis device is used for collecting sample liquid from the target sample rack at the sampling station and mixing the sample liquid with a reaction reagent to realize specific protein analysis. Manual operation can be avoided, the detection efficiency can be improved, and the accuracy of a detection result can be ensured.