342 results about "Specific protein" patented technology

The present invention relates to various molecular constructs having at least one cellular-targeting element, one protein-targeting element, and one ubiquitin ligase recruitment element, wherein these elements are conjugated in a manner that facilitates cellular uptake and targeted degradation of specific proteins within cells. The present invention further relates to multifunctional proteolysis-targeting chimera conjugates and uses thereof for the treatment of diseases or disorders such as immune and inflammatory diseases, infectious diseases, cardiovascular diseases, endocrine disorders, and various cancers that are otherwise resistant to traditional therapeutic regimens.

The invention provides a composition with oil-controlling, acne-removing, barrier-repairing effects and application thereof, and belongs to the field of cosmetics. The composition is prepared from a baropetalum beetle leaf extract, a roselle flower extract, totarol and a cryptomeria fortunei bud extract. The baropetalum chinense leaf extract has the effects of balancing grease secretion and shrinking pores; the roselle flower extract is rich in organic acid and anthocyanin, and has the effects of dissolving pore keratosuppository, controlling oil, resisting inflammation, smoothing acne skin, preserving moisture and removing redness; as a bacteriostatic agent, totarol has a natural preservative effect on gram-positive bacteria and partial negative bacteria, and has the effects of removing acnes, sterilizing, diminishing inflammation, whitening and the like; the cryptomeria fortunei bud extract can promote skin tissue structure regeneration and strengthen skin barriers. The composition effectively regulates and controls excessive secretion of sebaceous gland grease and pox skin specific protein through the synergistic effect of various components, promotes the health of pox skin, repairs the skin barrier and enhances the self-protection ability of the skin at the same time.

The invention belongs to the field of functional proteomics research, and particularly relates to a combined analysis method for analyzing interaction between a small molecule substance and a targeted protein, which is used for analyzing the small molecule substance and the protein by adopting LiP-MS (Limited Protein-Mass Spectrometry) technology and phosphorylated proteome combined analysis. According to the method, the optimized LiP-MS technology is adopted, the target protein can be directly recognized under the complex proteome background without enriching specific protein, and the problem that low-abundance protein is difficult to detect is solved; phosphorylated proteome abundance transformation protein is analyzed by combining high-resolution mass spectrometry, so that the recognition capability on a drug target and a shear variant thereof is enhanced, and the detection sensitivity is improved.

The invention relates to the technical field of bioactive peptides, in particular to a novel multifunctional collagen-like tripeptide and application thereof, an amino acid sequence contains multiple combinations, X can be selected from multiple components, 3-methoxy-4-hydroxyphenylalanine and the like are added, and the novel multifunctional collagen-like tripeptide can interact with specific proteins, activate related pathways and play multiple physiological functions and is applied to multiple fields. The collagen-like tripeptide has obvious advantages, innovates component expansion functions, improves the preparation method, improves the efficiency, reduces the cost, is matched with other components in the fields of beauty, health care and medicine, enhances the efficacy, realizes targeted delivery, and brings a new opportunity to related industries.

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention provides a tumor cell vaccine as well as a preparation method and application thereof. The preparation method comprises the following steps: acquiring and culturing tumor cells; enabling the cell membrane of the tumor cell to express a targeting antibody by utilizing a gene vector infection or gene editing technology, and enabling the targeting antibody to be used for enabling the tumor vaccine to be combined with the dendritic cell; performing overexpression of tumor specific protein on the cell membrane surface of the tumor cell by using a gene vector infection or gene editing technology; a nucleic acid substance of a target protein is wrapped by lipid nanoparticles to form a vaccine framework, and the nucleic acid substance can enable target cells to express directional chemotactic molecules; wherein the directed chemotactic molecule is used for transferring the dendritic cells to lymph nodes; the target vaccine is obtained by wrapping the vaccine framework with the cell membranes of the tumor cells, lymph node homing of the dendritic cells can be promoted, and the antigen presentation efficiency is increased and the immune effect is enhanced through tumor specific protein carried by the target vaccine and assisted by an immunologic adjuvant.

The invention relates to a UGT enzyme subtype substrate specificity prediction method and model. On the basis of a directional message passing neural network, graph structure characterization of a small molecule compound and features of specific protein binding sites of UGT enzyme are deeply fused, a bimodal prediction normal form of'molecule + protein binding sites' is designed, a deep learning model is constructed, conversion from compound center prediction to molecule-enzyme binding site comprehensive prediction is achieved, and the prediction accuracy is improved. And accurate classification prediction can be carried out on UGT enzyme substrates and non-substrates.

Provided are proteins capable of engaging NK cells in cancer therapy, especially multi-specific proteins capable of binding IL 15 receptor, NKp46, target antigen of interest, and optionally Fc gamma receptor such as CD16a. Also provided are NKp46-binding proteins. The proteins have utility in the treatment of diseases, such as cancer.

The invention relates to a urine specific protein detection sample quality screening system and screening method. The screening method comprises the following steps: firstly, creating a urine specific protein quality model; then collecting an image of a to-be-detected sample; and finally, analyzing and evaluating the sample to be detected according to the sample quality model and the image of the sample to be detected, and converting urine colors (such as light yellow, deep yellow, hematuria and the like) into quantifiable numerical values by collecting the image of the sample (urine). Compared with traditional naked eye observation, judgment deviation caused by subjective factors such as experience and ambient light of detection personnel is avoided, and the detection result is more objective and repeatable; according to the method, the sample image is converted into quantifiable numerical values so as to carry out numerical analysis on the detection data of the color, clarity and turbidity of the sample, hematuria and urine with high turbidity in the sample are automatically screened out, manual operation steps are reduced, the detection efficiency is improved, and the method is particularly suitable for clinical or large-scale screening scenes.

The utility model discloses a novel protein marker diagnostic kit for detecting the muscle injury degree, which comprises a detection kit for detecting the muscle injury degree and a dustproof cover assembled above the detection kit, the detection kit is provided with a detection port for dropping blood of a patient and a display port for displaying a diagnosis result, a reagent card for evaluating the muscle injury degree by detecting the concentration of a specific protein marker in the blood is arranged in the detection kit, and the detection end of the reagent card is located below the detection port. The display end of the reagent card is positioned below the display opening; a positioning opening for positioning the dustproof cover is formed above the detection kit, and a positioning block matched with the positioning opening is arranged in the dustproof cover. According to the utility model, the muscle injury degree of a patient can be detected by quickly reading the novel protein marker in the blood, so that an accurate diagnosis and treatment basis is provided for the treatment of the patient, the detection time of the patient is shortened, and the treatment cost is reduced.

The invention provides a recombinant exosome as well as a preparation method and application thereof. The preparation method of the recombinant exosome comprises the following steps: transfecting a target cell with an exosome loaded with nucleic acid molecules, and culturing to prepare the recombinant exosome; the nucleic acid molecule sequentially comprises a promoter sequence, a sequence for coding beta-globin, a 5 '-UTR, a target gene sequence, a sequence for coding alpha-globin, a 3'-UTR and a terminator sequence from the 5'end to the 3 'end. The method not only can be used for producing the recombinant exosome of the specific protein, but also has high flexibility and controllability. The recombinant exosome produced by the preparation method disclosed by the invention shows good stability and biological activity in cell culture and in-vivo experiments, and therapeutic protein can be effectively delivered to target cells. According to the technology, a virus-free and non-integrated treatment method is provided, and common risks in traditional gene therapy are reduced.

The invention is applicable to the technical field of vaccines, and provides a haemaphysalis unguiculata salivary gland multi-component subunit vaccine as well as a preparation method and application thereof, the effective components of the vaccine comprise rHlPDI-2 and rHlPrx soluble recombinant proteins, and the amino acid sequences of the rHlPDI-2 and rHlPrx soluble recombinant proteins are shown as SEQ ID NO.1-2. Starting from salivary gland secretory protein related to tick activity in a host biting process of haemaphysalis unguiculata, special protein of salivary glands of tick species is researched, the recombinant protein vaccine is prepared, survival and reproduction of haemaphysalis unguiculata can be affected, and the prepared multi-component subunit vaccine has an interference effect on biting and passage of haemaphysalis unguiculata and can be used for preparing the multi-component subunit vaccine for preventing and treating haemaphysalis unguiculata. The anti-tick vaccine has a good anti-tick effect, contributes to prevention and treatment of tick-borne diseases, and lays a foundation for development of other tick related vaccines.

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

The invention relates to the technical field of sample analysis, and discloses a specific protein detection method based on double-angle scattering turbidimetry, which comprises the following steps: S1, obtaining a small-angle signal concentration relation curve and a medium-angle signal concentration relation curve, and obtaining a cross threshold signal; s2, the laser light source irradiates the reaction cup from one side of the reaction cup along the horizontal axis, the detection module is located on the other side of the reaction cup and opposite to the laser light source, and the detection module collects small-angle and medium-angle scattering signals at the same time and transmits collected scattering signal information to the control module; and S3, the control module compares the received medium-angle scattering signal information with the cross threshold signal information, dynamically selects and adopts a small-angle scattering signal, and obtains the concentration of the specific protein to be detected according to a small-angle signal concentration relation formula. Or obtaining the concentration of the specific protein to be detected by adopting the medium-angle scattering signal according to a medium-angle signal concentration relation formula. The method can improve the accuracy of protein concentration analysis.

The invention relates to a method for separating out and condensing specific protein in liquid for dyeing and detection. The liquids include but are not limited to human samples, human blood, saliva, urine and the like. The liquid is diluted and its pH is raised or lowered to form a mixed liquid. Placing the mixed solution in an electrostatic field, moving the specific protein in the mixed solution to one side of the mixed solution, accurately dropwise adding an acidic or alkaline solution, adjusting the pH value of the mixed solution, enabling the pH value of the mixed solution to reach the isoelectric point of the specific protein, and enabling the specific protein to be separated out and precipitated on one side of the mixed solution. In addition, according to the sequence of the pH values from large to small or from small to large, the isoelectric points of all the specific proteins can be traversed in the process, and all the specific proteins can be separated out and precipitated in the same area of the mixed solution.

The disclosed systems and methods involve the semi-quantitative detection of disease-specific protein signatures in biological samples. The system may include a housing with a motor driven substrate holder on rails. The substrate holder is configured to be positioned at multiple locations along the guide rails. Imaging sensors capture images of portions of the substrate at these locations. The system detects markers on the substrate and determines the location of wells based on these markers. Based on the determined location of the wells, color values for each pixel in the wells can be measured. These values may be analyzed to detect disease-specific protein signatures in a variety of sample types.

The present invention relates to a method for transforming a strain of a lactic acid bacterium, such as of the Lactococcus genus through competence for natural transformation. The present invention further relates to strains obtained or obtainable by this method. The present invention also relates to the use of specific strains of a lactic acid bacterium, such as of the genus Lactococcus having inherently low, reduced or disrupted activity of specific proteins for transforming these strains through natural transformation. The present invention further relates to methods for increasing the rate of natural transformation by increasing competence for natural transformation of a strain of a lactic acid bacterium, such as of the genus Lactococcus and for identifying a strain of a lactic acid bacterium, such as of the Lactococcus genus which is transformable through competence for natural transformation.

The invention relates to the technical field of medical instruments, in particular to a specific protein analysis system. The system comprises a sample management device, a sample conveying device and a protein analysis device, the sample management device is used for storing a plurality of sample racks and conveying a target sample rack to a waiting station; the sample conveying device is used for conveying the target sample frame at the waiting station to the sampling station and conveying the target sample frame at the sampling station back to the waiting station; and the protein analysis device is used for collecting sample liquid from the target sample rack at the sampling station and mixing the sample liquid with a reaction reagent to realize specific protein analysis. Manual operation can be avoided, the detection efficiency can be improved, and the accuracy of a detection result can be ensured.

The present invention relates to a modified mitochondrial comprising, on the outer membrane of the mitochondrial, an antibody that binds to a cancer cell-specific protein and a cytosine deaminase. It is proved that when the mitochondria and 5-FC are jointly used for treating a pancreatic cancer cell line, the cytotoxicity induced by 5-FC treatment is remarkably enhanced. The effect is proved as follows: 5-FC is converted into 5-FU by cytosine deaminase expressed on a mitochondrial outer membrane, so that pancreatic cancer cells are induced to generate apoptosis. Therefore, the modified mitochondria according to the present invention can be effectively used in anticancer therapy using 5-FC as an anticancer agent.

The present invention relates to an RT-qPCR detection method and application for the distribution of human mesenchymal stem cells in mice, belonging to the field of biomedicine technology. The RT-qPCR detection method comprises the following steps: sampling: taking organ tissues of mice injected with human mesenchymal stem cells, extracting their total RNA for testing; detection: using real-time fluorescence quantitative PCR to detect the human mesenchymal stem cell-specific protein gene sequence in the above-mentioned total RNA to obtain the distribution of human mesenchymal stem cells in mice. The above-mentioned detection method can achieve the purpose of quickly and accurately detecting the in vivo distribution of multiple tissues at different time points, providing data support for the selection of stem cell products, and being used in the evaluation of the distribution of stem cells in the body.

The invention relates to a binding protein B capable of binding with klebsiella pneumoniae and application of the binding protein B, and belongs to the technical field of molecular biology and protein engineering. The binding protein B provided by the invention can be specifically bound with an extramembrane part of a specific protein P24017. OMPAKLEPN of klebsiella pneumoniae, and experiments such as bimolecular fluorescence complementation and flow cytometry prove that the binding protein B has good recognition and stable binding capacity on the klebsiella pneumoniae, can be used for identifying and screening the klebsiella pneumoniae, and has good application prospects. And a key tool is provided for targeted drug development aiming at the bacterium.

The invention discloses a calibrator or a quality control product for an exosome membrane marker detection reagent based on an engineering bacteriophage, the engineering bacteriophage is a bacteriophage capable of simultaneously displaying more than two exosome membrane markers, and the bacteriophage comprises any one of M13, T4, T7 or lambda bacteriophage; the exosome membrane marker comprises at least two of an exosome transmembrane transporter, an exosome specific protein or a disease-related marker. The invention relates to a calibrator or a quality control product for an exosome membrane marker detection reagent based on engineering bacteriophage, which is a low-high-value calibrator or quality control product obtained by diluting the engineering bacteriophage by using a diluent in proportion, and solves the problems of high cell culture and extraction cost, large batch difference, low cost and the like when an exosome is biologically extracted as the calibrator or quality control product for the detection reagent. The problems of poor stability in the use process and the like are solved, and the problems of calibration of a traceable value source and quality control of an exosome membrane marker in-vitro detection kit are solved.

The application discloses a Campylobacter-specific protein antibody, an antibody-coupled magnetic bead and application. The antibody is a monoclonal antibody 1A10 and a monoclonal antibody 3D9, the monoclonal antibody 1A10 comprises a light chain variable region and a heavy chain variable region, the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 1A10 are shown in SEQ ID NO: 7 and 8 respectively; the monoclonal antibody 3D9 comprises a light chain variable region and a heavy chain variable region, the amino acid sequences of the light chain variable region and the heavy chain variable region of the monoclonal antibody 3D9 are shown in SEQ ID NO: 9 and 10 respectively. The antibody-coupled magnetic bead is coupled by the monoclonal antibody 1A10, the monoclonal antibody 3D9 and activated carboxyl magnetic beads respectively. The antibody-coupled magnetic bead can specifically enrich Campylobacter, has high enrichment efficiency, simple enrichment operation, can be applied to metabolic product analysis of Campylobacter, and can be used for qualitative and quantitative analysis of the Campylobacter.

Single-cell multi-omics by co-encapsulating a single cell with two beads, the first an RNA barcoding bead having barcoded mRNA capture primer oligonucleotides attached on the bead surface; and the second a DNA barcoding bead having two types of oligonucleotides releasably attached to the surface: (1) barcoded adapter oligonucleotides that are complementary to oligonucleotides bound to the transposase that are eventually incorporated into gDNA fragments and (2) polyadenylated barcoded oligonucleotides containing the same barcode sequence as the adapters. In addition, integrated analysis of RNA and protein, including intracellular protein, from individual cells using similar co-encapsulation of a single cell, an RNA barcoding bead, and with / without a specific or non-specific protein binding bead in a microwell, to avoid protein fixation by first lysing the cell to liberate intracellular contents, and then capturing protein either on a solid surface or in solution with barcoded affinity reagents.

The present invention relates to pharmacogenetics, more specifically to strategies involving biomarkers associated with the clinical response to a compound before or during treatment of a cartilage disorder, such as osteoarthritis. The present invention more particularly relates to the combination of JSW measurements and level of specific proteins present in the blood, serum, synovial fluid or in the urine, which can be used in strategies such as patients' enrichment in clinical trials, patients' selection strategy before or during treatment or for adapting the treatment of a patient in the frame of treatments for cartilage disorder, such as osteoarthritis.

This invention discloses an antibody targeting a specific protein of *Lactobacillus* and its applications. The monoclonal antibody of this invention is any one of monoclonal antibody 1B3, monoclonal antibody 3B5, monoclonal antibody 2E5, and monoclonal antibody 3D8, which respectively recognize proteins Srt A, Cps C, ENO 1, and Fts Z. The magnetic bead antibody conjugate is prepared by conjugating the monoclonal antibody with activated carboxyl magnetic beads. The magnetic bead antibody conjugate of this invention can specifically enrich *Lactobacillus* bacteria with high enrichment efficiency and simple operation. It can be applied to the identification of metabolites of *Lactobacillus* bacteria, as well as their qualitative and quantitative analysis.

Using a novel dependence on plasmid-mediated expression (DOPE) technology, conditional depletion of GrgA, a Chlamydia-specific protein, has been demonstrated to result in greatly reduced reticulate body (RB) proliferation rate and near complete lack of elementary body (EB) formation, thus disrupting the normal chlamydial developmental cycle. This conditional GrgA-deficient Chlamydia allows study of chlamydial growth and developmental regulation and can be used as the basis of an attenuated, maturation-defective but immunogenic bacteria for use as an avirulent vaccine against Chlamydia.

The present invention discloses an antibody against Clostridium difficile specific protein, a magnetic bead-antibody conjugate and its application. The monoclonal antibody is any one of monoclonal antibody CD1, monoclonal antibody CD2, monoclonal antibody CD3 and monoclonal antibody CD4. The magnetic bead-antibody conjugate is formed by conjugating a monoclonal antibody with activated carboxyl magnetic beads to obtain the magnetic bead-antibody conjugate. Based on the magnetic bead-antibody conjugate sorting method, antibody magnetic beads that couple and recognize the Clostridium difficile secreted proteins TcdA, TcdB, the highly expressed GDH on the surface of Clostridium difficile, and the LPxTG of Clostridium difficile are used to capture Clostridium difficile, achieving the purpose of rapidly isolating Clostridium difficile from fecal samples. The present invention is based on the immunomagnetic bead separation technology of antigen-antibody reaction for separation, with the characteristics of being rapid, simple, having high enrichment efficiency and strong specificity. The present invention can be used to isolate and remove Clostridium difficile in fecal microbiota transplantation, and can also be used for strain identification and sequencing.

A compound of general formula (I), a compound of general formula (III) and anti-tumor applications thereof. A series of compounds designed and synthesized have a wide range of pharmacological activities, have the function of degrading specific proteins and / or inhibiting activity, and can be used in related tumor and cancer treatments.

The application provides a kind of liposome that can enhance organ targeting, preparation method and application, belongs to the field of biological medicine and nanotechnology.The application first proposes the strategy of actively regulating LNP organ targeting by pre-assembling specific protein crown in vitro, which breaks through the technical limitations of traditional passive formation of natural protein crown.Through selecting main lipids (CDO, CDA, EDM) with different head chemical structures, precise control of protein crown composition is realized.Furthermore, the method of the application is simple in process and mild in condition, only needs standard in vitro incubation steps, and has great clinical transformation potential.The pre-assembled protein crown method provided by the application significantly enhances the organ targeting efficiency of LNP: after pre-incubation of LNP1 with APOA1, the enrichment degree of spleen is increased by 2.8 times;after pre-incubation of LNP2 with VTN, the enrichment degree of lung is increased by 52%;after pre-incubation of LNP3 with APOE, the enrichment degree of liver is increased by 1.2 times.