4 results about "Hpv genotypes" patented technology

The invention relates to a method, uses, a system, and a kit for detecting a human papillomavirus (HPV) genotype associated with the development of cervical, oral, or anogenital cancer in a subject and for determining the HPV genotype or genotypes thus detected in the same. Additionally, the present invention relates to uses of direct and / or reverse amplicons or primers generated by multiplex real-time PCR comprising a molecular tag for identifying a marker of human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and / or 68.

The invention relates to the technical field of machine learning, in particular to a cervical cancer assessment method based on high-risk HPV genotyping data. The method comprises the following steps: collecting cervical cancer high-risk HPV genetic typing sample data; performing data preprocessing on the sample data; segmenting the sample data into model training data, model calibration data and model verification data by adopting an equal-proportion layered random sampling method, and constructing a cervical cancer positive probability prediction model according to the model training data; and predicting to-be-detected sample data according to the cervical cancer positive probability prediction model. According to the technical scheme, the cervical cancer positive probability prediction model is constructed based on the high-risk HPV genotyping sample data and the machine learning method, the prediction accuracy is improved by optimizing the model, the method can be applied to early screening of cervical cancer, and valuable auxiliary reference information is provided for clinicians.

The present invention provides a composition, a kit and a method for detecting HPV genotypes. The present invention optimizes respectively from aspects of urine extraction and PCR sensitivity, develops a method for extracting high-quality DNA from 40 mL of whole urine, and optimizes and screens a set of probe primer combinations with high sensitivity. By using this extraction method and combining with the screened probe primer combinations, it is possible to accurately and effectively detect a total of 15 HPV types including HPV type 53 and genotype HPV16 / 18 with only one-tube multiplex PCR reaction in urine with low HPV content, and the lower limit of detection LOD in urine samples reaches 10-50 copies / mL, having good sensitivity and specificity.

This invention discloses a rapid on-site HPV genotyping method based on one-pot RPA-Cas12a, belonging to the field of molecular biology diagnostic technology. This invention utilizes the automated target screening tool AutoCORDS to design specific crRNAs and RPA primers for different HPV subtypes. Combined with RPA amplification and CRISPR-Cas12a, it achieves highly sensitive, highly specific, efficient, and convenient HPV genotyping detection. This method requires no complex instruments and has advantages such as simple operation, fast analysis speed, and independence from expensive equipment. It can accurately detect genotypes of 14 high-risk HPV types and is suitable for screening and diagnosis of HPV infection in resource-scarce areas.