203 results about "Cervical ca" patented technology

The invention relates to the technical field of biological medicine, and discloses an aromatic polyketone compound as well as a preparation method and application thereof. According to the aromatic polyketone compound, a compound I and a compound II are separated from an ethyl acetate extract of rice fermentation liquor of Crystalomyces hyricus, and the compound I and the compound II are respectively named as Crystaloketide A and Crystalomycin D. In-vitro anti-cancer activity tests are carried out on the Crystaloketide A and the Crystalomycin D. According to the aromatic polyketone compound disclosed by the invention, the anti-cancer activity of the Crystaloketide A and the Crystalomycin D. Results show that the compound I and the compound II have the effects of inhibiting human cervical cancer cells, human liver cancer cells, human nerve cancer cells and human pancreatic cancer cells respectively, so that the compound I and the compound II can be further prompted to be used for preparing anti-cancer drugs.

The invention provides a cervical cancer self-adaptive radiotherapy assessment decision method and device, and relates to the technical field of medical artificial intelligence, and the method comprises the steps: obtaining a cone beam CT image and a plan CT image before radiotherapy of a patient to be subjected to radiotherapy on the day of radiotherapy, and extracting the structural quantitative features of the images; the method comprises the following steps: calculating a difference value of structured quantitative characteristics between a cone beam CT image on the day of radiotherapy and a plan CT image before radiotherapy to obtain multi-dimensional geometric change characteristics; and inputting the multi-dimensional geometric change features, the vectorized representation of the predetermined key region of the cone beam CT image and the clinical text information into a pre-trained clinical large language model, and outputting a self-adaptive radiotherapy demand tag. The problem that the prior art depends on manual analysis, and it is difficult to compare the change characteristics of the CT image between the radiotherapy day and the plan before radiotherapy to automatically analyze whether self-adaptive radiotherapy is executed on the radiotherapy day is solved.

The invention belongs to the technical field of medical equipment, and particularly discloses a cervical thermocoagulation therapeutic apparatus for cervical cancer treatment and a use method thereof.The cervical thermocoagulation therapeutic apparatus for cervical cancer treatment comprises an apparatus body, and the apparatus body is connected with a thermocoagulation handle device and a device for thermocoagulation in a cervical area through a connecting line; the thermocoagulation handle device comprises a handle connected to one end of the connecting line, and the handle is connected with a treatment head through a slender shaft and used for treatment. By means of the fixing structure and the moving structure, connection between the sealing piece and the slender shaft can be kept sealed and stable, sealing reliability is guaranteed, the treatment head can be taken down from the sealing piece only by moving the moving structure, then a new treatment head or treatment heads of different models are installed, the pollution risk is reduced, and then replacement efficiency is improved conveniently.

The invention discloses application of a tripterygium wilfordii exosome in preparation of an anti-cervical cancer medicine and a tumor angiogenesis inhibiting medicine, and relates to the field of biological medicine. The medicinal plant exosome derived from tripterygium wilfordii is successfully separated, and it is proved that the medicinal plant exosome can regulate related pathways through delivery of functional nucleic acid molecules to inhibit proliferation and migration of cervical cancer cells and promote active oxygen related apoptosis so as to be used for tumor treatment. The tripterygium wilfordii exosome can further act on vascular endothelial cells in a tumor microenvironment, the migration function of Hela cells is inhibited, and then the effect of resisting cell proliferation in the tumor microenvironment is achieved. The tripterygium wilfordii exosome has the advantages of being simple in preparation method, remarkable in tumor growth resistance and tumor cell migration inhibition effect, good in biocompatibility, high in delivery efficiency and the like, and can be used as a novel nano-drug for tumor treatment.

The invention relates to the technical field of medical information processing, in particular to a cervical cancer cell early screening and intelligent evaluation system based on multi-modal data, which comprises a data acquisition module, a form detection module and a screening early warning module, the data acquisition module is used for acquiring multi-modal data of a current detection object in a plurality of inspection processes; the morphology detection module is used for calculating a cellular morphology score of a current detection object in any target inspection process; the screening early warning module is used for determining similar detection objects of the current detection object, and correcting differences between the current detection object and the similar detection objects in the aspects of cellular morphology scores and lesion detection indexes according to the approximation degree between different types of lesion detection indexes of the current detection object; and obtaining the screening early warning coefficient of the current detection object by using the relative change condition of the correction result. According to the invention, the early screening and evaluation effects on cervical cancer cells are improved.

This invention belongs to the field of organic synthesis and relates to a nitrogen-containing [3,2-b]indole derivative, its preparation method, and its application. The nitrogen-containing [3,2-b]indole derivative is a compound, optical isomer, or pharmaceutically acceptable salt thereof, as shown in the following structure: ; wherein R1, R2, R3, and R4 are independently selected from hydrogen atoms, halogens, cyano groups, C1-C8 alkyl groups, and C1-C8 alkoxy groups; R5, R6, R7, and R8 are independently selected from hydrogen atoms, halogens, and C1-C8 alkyl groups; R9 is selected from C1-C8 alkyl groups; when R1, R2, R3, R4, R5, R6, R7, and R8 are all hydrogen atoms, R9 is selected from C1-C2 alkyl groups. The compound exhibits antitumor activity, particularly against HeLa cervical cancer, showing a significant inhibitory effect on its proliferation.

HPV16 is a main cause of malignant tumors such as cervical cancer, and E6 protein of HPV16 becomes a key treatment and diagnosis target. In the prior art, an HPV16 detection antibody has the problems of poor stability, dependence on cold chain transportation, low preparation efficiency and the like. According to the invention, a nano antibody sequence which is high in affinity (dissociation constant KD is less than or equal to 1 * 10 <-7 > M) and stable at room temperature is obtained through total synthesis library construction and a phage-assisted directed evolution (PACE) screening technology. After the antibody is stored at 25 DEG C for 30 days, the activity of the antibody is kept to be greater than or equal to 95%, the antibody can be directly used for household test paper, the accuracy rate reaches 98%, and meanwhile, the antibody can be expanded to medical applications such as CAR-T cell therapy. Compared with a traditional antibody, the antibody has the advantages of being short in preparation period, low in cost, wide in application scene and the like, and a breakthrough solution is provided for early screening of cervical cancer.

The invention discloses a cervical cell segmentation method combining semantic condition diffusion and knowledge verification, which comprises the following steps: S1, constructing a cervical cancer diagnosis vertical field multi-modal large model, and performing fine tuning on an open-source multi-modal large model through a low-rank adaptation supervision fine tuning method to obtain the cervical cancer diagnosis vertical field multi-modal large model; s2, generating a semantic vector for guiding segmentation; s3, performing preliminary segmentation based on a conditional diffusion model, performing iterative denoising on the to-be-segmented image, and generating a preliminary cervical cell segmentation mask; and S4, performing segmentation post-processing based on domain knowledge: performing morphological processing on the preliminary segmentation mask to obtain a final cervical cell segmentation result. According to the method, large-scale text data can be fully utilized and converted into refined semantic information for guiding image segmentation, so that the challenge of scarcity of labeled samples is overcome, the performance, generalization ability and clinical applicability of a cervical cell abnormal region segmentation model are remarkably improved, and finally more accurate and more reliable cervical cell pathological diagnosis assistance is realized.

The invention relates to compositions containing selenite-containing compounds and pharmaceutically acceptable acids, selected from citric acid, acetic acid, malic acid, carbonic acid, sulphuric acid, nitric acid, hydrochloric acid, fruit acids or mixtures thereof, for use for treating cervical inflammations, dysplasia and / or carcinomas. The invention further relates to methods of using such compositions.

The invention relates to an application of a DNAJB4 gene and an encoding protein thereof in preparation of a product for enhancing cancer radiotherapy sensitivity. The DNAJB4 gene provided by the invention is closely related to cancer radiotherapy sensitivity, and overexpression of the DNAJB4 gene is positively related to radiotherapy sensitivity of head and neck squamous cell carcinoma, cervical cancer or breast cancer. A product prepared from the target DNAJB4 selectively degrades DNA-PKcs through a CMA way, high specificity is achieved, and toxicity to normal tissue can be reduced; the radiotherapy remission rate of a patient with high DNAJB4 expression is obviously improved (clinical queue data); the combined treatment prolongs the median lifetime of the mouse model; the drug resistance risk can be reduced by combining with existing therapies (radiotherapy and immunotherapy).

The application discloses a dihydroquinazolinone-containing N-hydroxy amide compound and a preparation method and application thereof, relates to the technical field of medicinal chemistry, and utilizes the splicing principle to design and synthesize dihydroquinazolinone-containing N-hydroxy amide compounds with novel structures, and the compounds are tested in terms of HDAC6 protein affinity and anti-proliferation activity of cervical cancer cell Siha, and the results show that the compounds E1-E5 have better affinity to the HDAC6 protein, the IC 50 values are 4.1-85.4 nM, wherein the affinity of the compound E4 to the HDAC6 protein is higher than that of a positive control SAHA; and the compounds E1-E5 can better inhibit the proliferation of the cervical cancer cell Siha, the IC 50 values are 2.4-9.3 muM, wherein the activity of the compound E4 is the strongest.

This invention provides a physiotherapy system for pelvic floor function rehabilitation in cervical cancer patients, relating to the field of pelvic floor physiotherapy. The system includes: a data acquisition module for acquiring pelvic floor electromyography (EMG) and ultrasound data from multiple historical patients; a data analysis module for constructing an EMG atlas and an EMG-factor correlation atlas based on the aforementioned data; the data acquisition module is also used to acquire the current patient's pelvic floor EMG data based on the EMG atlas, and to acquire the current patient's pelvic floor ultrasound data based on the current patient's pelvic floor EMG data and the EMG-factor correlation atlas; a treatment plan determination module for determining the current patient's physiotherapy plan based on the current patient's pelvic floor EMG and ultrasound data; and a physiotherapy execution module for controlling the physiotherapy equipment to perform pelvic floor function rehabilitation physiotherapy based on the current patient's physiotherapy plan, thereby improving the flexibility and effectiveness of pelvic floor function rehabilitation physiotherapy for cervical cancer patients.

This invention belongs to the field of biomedical technology, and particularly relates to a pharmaceutical composition for treating cervical cancer and its preparation method. The pharmaceutical composition for treating cervical cancer of this invention comprises the following components: a apigenin derivative and acetylvalerin in a mass ratio of 1:(0.3~0.5). This invention obtains a apigenin derivative by introducing specific functional groups onto apigenin, which exhibits high pharmacological activity against cervical cancer; synergistically acting with acetylvalerin, it effectively kills cervical cancer cells while reducing damage to normal cells, thus providing a safer and more effective treatment option for cervical cancer patients.

The invention relates to a cervical cancer auxiliary classification method and device based on multi-task learning and a medium. The method comprises the following steps: preprocessing an input target medical image; inputting the preprocessed target medical image into a feature extraction module to extract an initial feature map; performing channel interaction and feature scale adjustment on the initial feature map through a plurality of feature extraction layers in sequence; inputting the processed initial feature map into a segmentation branch, and generating a segmentation feature map through convolution and up-sampling operation; extracting a feature map of the classification branch from the feature extraction layer, and adding and fusing the segmented feature map and the feature map of the classification branch element by element; performing average pooling operation on the fused feature map; and inputting the feature map after the average pooling operation into a full connection layer, and outputting a classification result of the target medical image. By constructing a multi-task collaborative architecture design, segmentation and classification tasks are processed in parallel on the basis of a shared backbone network, and feature multiplexing and improvement of calculation efficiency are achieved.

The invention discloses an mRNA (messenger Ribonucleic Acid) quality control product of human papilloma virus as well as a preparation method and application thereof, and belongs to the technical field of in-vitro diagnosis quality control. The method comprises the following steps: chemically synthesizing an HPV E6 / E7 gene DNA sequence, respectively cloning the HPV E6 / E7 gene DNA sequence into a cloning vector to construct a synthetic plasmid template, further preparing a nucleic acid fragment containing E6 / E7 mRNA through in-vitro transcription, carrying out absolute quantification through a digital PCR technology, mixing the nucleic acid fragment with human cervical cancer cell line RNA without any HPV sequence, and screening to obtain the HPV E6 / E7 gene DNA sequence. And the mRNA quality control product with stable structure and accurate value is prepared through a vacuum freeze drying technology. The quality control product can simulate HPV E6 / E7 mRNA in a clinical sample, has comprehensive types, is suitable for accurate evaluation of sensitivity, specificity, precision and other analysis performances of an HPV E6 / E7 mRNA detection kit, provides a key quality control tool for accurate risk stratification of cervical cancer, and is suitable for various detection platforms.

The application relates to a kind of mitochondria-based single cell feature extraction and analysis method and system, comprising: obtaining the multiple modal images such as bright field image, nucleus fluorescent image and mitochondria fluorescent image of single cell;Image preprocessing is carried out to the three modal images obtained;For different structures such as mitochondria, morphological and texture features are extracted, and feature analysis is carried out;Further, through the fusion of mitochondria and machine learning technology, the automatic classification of cells is realized.The application is used for the classification of human cervical epithelial cells (H8) and cervical cancer cells (HeLa), and the machine learning analysis of morphological features and texture features shows the potential of mitochondria in the classification of cervical cells.The application has strong applicability, can be combined with machine learning and other analysis methods, and can be applied to various biological cells, has universality, and is easy to popularize.

The application discloses a cervical cancer methylation detection kit and application thereof, the kit takes PAX5 gene, PAX1 gene, MICOS10-NBL1 gene and MICOS10 gene as detection targets; the kit mainly comprises specific primer probe dry powder combination, PCR reaction reagent dry powder, positive control product dry powder, negative control product dry powder and microdroplet generation oil. The sample is detected by bisulfite conversion, and the kit is combined with microdroplet digital PCR detection technology to realize accurate detection of the methylation level of the target gene. The detection verification result shows that the sensitivity of the kit for cervical cancer detection reaches 96.9%, the specificity reaches 95.7%, the kit has the advantages of accurate detection, simple operation and strong stability, can be widely applied to early screening, clinical auxiliary diagnosis and physical examination of healthy people of cervical cancer, and provides reliable technical support for early discovery and early treatment of cervical cancer.

Disclosed herein are compositions and methods of manufacturing, testing, and using a biocompatible and disposable vaginal suppository, comprising chitosan, collagen, carboxymethyl cellulose, and an enzyme, capable of breaking down vaginal mucous and releasing the active ingredients consistently over extended periods of time. Methods disclosed herein may also include the use of these compositions, methods, and devices for treating vaginal diseases, such as vaginitis, genitourinary syndrome, and other conditions, such as vulvar and vaginal cancer, or cervical cancer.

The invention discloses fluoroalkyl substituted azafluorene and pyridopyrimidino isoindole compounds as well as a synthesis method and application thereof, and belongs to the technical field of organic synthesis and drug discovery. The preparation method comprises the following steps: mixing a 3-alkenyl-1, 2, 4-oxadiazolone compound 1, a fluoroalkyl acetylenic ketone compound 2, a catalyst, an additive and an organic solvent, and carrying out heating reaction to prepare a fluoroalkyl substituted azafluorene compound 3 and / or a fluoroalkyl substituted pyridopyrimidino isoindole compound 4. Experimental research discovers that the compound disclosed by the invention has the activity of obviously inhibiting proliferation of Hela and HCT-116 cancer cells, so that the compound disclosed by the invention has obvious anti-cancer activity on cervical cancer and / or colon cancer, has potential medicinal value, and provides a new structural unit for drug screening.

The application discloses a marker group for cervical cancer screening and diagnosis and application thereof, and belongs to the field of molecular biological technology.The marker group comprises at least four polypeptides shown in sequences of SEQ ID NO.1-42.The marker group constructed by the application has more excellent sensitivity and specificity when used for auxiliary diagnosis and early screening of cervical cancer.The auxiliary diagnosis result is superior to the efficiency (sensitivity 81.1%, specificity 78.9%) of combined detection of serum CEA, SCC-Ag and CA125 in early diagnosis of cervical cancer;the diagnostic sensitivity and specificity of serum cf-DNA are 72.2% and 81.7% respectively.The early screening result is also superior to the internationally recognized cervical cancer screening method, i.e., combined screening of cytological detection (TCT) and human papilloma virus (HPV) virology detection.

This invention relates to a hydrogel isolation and protection device for cervical cancer brachytherapy and its preparation method. The preparation method includes the following steps: S1. Obtaining dried sodium polyacrylate particles and polyacrylamide powder separately, and mechanically mixing them uniformly in a dry state to obtain a highly absorbent dry-state mixed filler; S2. Preparing a polymer bag using a polymer material; S3. Inserting the outlet end of a water injection conduit into the polymer bag, and filling the polymer bag with the dry-state mixed filler; S4. Sealing the open end of the polymer bag to the outer wall of the water injection conduit to form a closed isolation device. The entire preparation process is carried out in a non-sterile environment, and microbial control of the device is limited to the outer surface of the polymer bag. This hydrogel isolation and protection device can be prepared using the above method.

The invention relates to the technical field of medicines, in particular to an enantiomer-atesane type diterpenoid compound as well as a preparation method and an application of the enantiomer-atesane type diterpenoid compound. The enantiomer-atesane diterpenoid compound disclosed by the invention is derived from plants, has anti-tumor activity, has cytotoxicity to human leukemia cells, human liver cancer cells and human cervical cancer cells, and is relatively good in inhibitory activity. Wherein the compound 3 has the best effect, the IC50 values of the compound 3 to three human tumor cells are 7.5 + / -1.2 micromole per liter, 9.8 + / -1.0 micromole per liter and 10.1 + / -0.9 micromole per liter respectively, and the activity of the compound 3 is equivalent to that of a positive control drug mitomycin. Particularly, the compound 3 also has good cytotoxicity to drug-resistant liver cancer cells (Huh7-LR cells), and the IC50 value of the compound 3 is 9.6 + / -1.1 micromole per liter. The compound 3 shows huge potential as a lead compound by virtue of the novel chemical structure and potent cytotoxicity shown in various cell lines.

The present application relates to the technical field of rehabilitation equipment, and particularly relates to a cervical cancer postoperative rehabilitation exercise device. The present application comprises a seat, and fixing frames fixed at two ends of the bottom of the seat, the bottom of the two fixing frames is fixed with a base, a rehabilitation exercise mechanism is assembled between the seat and the fixing frames, the rehabilitation exercise mechanism comprises a force applying assembly and a force guiding assembly, and two movable rods are slidably connected to one side of the seat. Through the structural design of the rehabilitation exercise mechanism and the auxiliary force applying mechanism, the present device is convenient for the rehabilitation exercise of patients in the early stage of exercise, is convenient for assisting the patients to exert force on the crotch, can effectively help the patients to overcome the early exercise, accelerates the work efficiency, improves the flexibility of the present device, and through the structural design of the cooperation mechanism, the present device is convenient for encouraging the patients in the process of rehabilitation exercise, can effectively stimulate the exercise enthusiasm of the patients, and is convenient for promoting the comprehensive rehabilitation of the cervical cancer postoperative patients.

The invention provides a dual-targeting nano-material photosensitizer for cervical cancer as well as a preparation method and application of the dual-targeting nano-material photosensitizer. The folic acid modified poly-beta-cyclodextrin (poly-beta-CD) is used as a nano-carrier and is used for loading a photosensitizer PAP (PAP is pyropheophorbide a covalently connected PAAKRVKLD) coupled with a nuclear localization signal (NLSs). The finally prepared FA-CD-coated PAP nano material can specifically recognize cervical cancer cells of overexpressed folate receptors (FR), so that accumulation in tumor cells is remarkably enhanced. In addition, the encapsulated PAP can achieve accurate cell nucleus localization. Under an illumination condition, reactive oxygen species (ROS) generated by PAP accumulated in nuclei can instantly oxidize and damage a DNA chain or DNA repair enzyme, so that cell death is directly induced, and the anti-tumor effect of targeted photodynamic is greatly enhanced. The novel dual-targeting nano drug-loading system prepared by the invention solves the problems of poor targeting and biological solubility and limited treatment effect of a traditional photosensitizer, and provides an innovative intelligent targeting delivery strategy for in-situ cell nucleus photodynamic therapy.

This invention relates to the field of camel thorn separation and purification technology, specifically to unsaturated ketone components from camel thorn, their separation and extraction methods, and their applications. The unsaturated ketone component in camel thorn is (7S,3E,5Z)-7,8-dihydroxy-6-methylocta-3,5-dien-2-one. This invention discloses for the first time the unsaturated ketone components in camel thorn, and in vitro antitumor pharmacodynamic experiments were conducted on these components. The experiments showed that the unsaturated ketone components in camel thorn have a certain inhibitory effect on human HeLa cervical cancer cells, thus enabling their application in the preparation of drugs for the prevention / treatment of cervical cancer.

The problem to be solved by the present invention is to provide more and better options for treating any solid tumor with high frequency microsatellite instability (high frequency MSI or MSI-H) alterations, cervical cancer, endometrial cancer, lung cancer, brain cancer and breast cancer.SOLUTION: The present invention relates to multispecific antibodies comprising an antigen binding site that binds an extracellular part of CD137 and an antigen binding site that binds an extracellular part of a second membrane protem for use in a method of treatment of cancers in a subject in need thereof. The invention further relates to such multispecific antibodies, as well as methods and other aspects related thereto.SELECTED DRAWING: None

The application discloses a cervical cancer circRNA marker and application thereof, and belongs to the technical field of biology. The inventors have found that the expression level of circular RNA hsa_circ_0002828 in the plasma of a cervical cancer patient is significantly lower than that in the plasma of a healthy person, thereby indicating that hsa_circ_0002828 can be used as a diagnostic marker for cervical cancer and for predicting the occurrence of cervical cancer. Meanwhile, after overexpression of hsa_circ_0002828, the proliferation ability and invasion ability of cervical cancer cells can be significantly inhibited, and tumor cell apoptosis can be strongly induced. These results show that hsa_circ_0002828 can be used as a tumor diagnosis marker and a potential target for tumor treatment, and is expected to be applied to the diagnosis and treatment of cervical cancer.

The application relates to the technical field of medical image database, and discloses a standardized cervical cancer pathological image database construction method, which comprises the following steps: step 1, specimen number is hashed to obtain pseudo-anonymous specimen number, unique slice identification and cell-level unique identification are generated; step 2, a unified reference system is established, the reference layer pixel spacing, the down-sampling multiple and the coordinate mapping are determined; step 3, the image is converted into an optical density space and normalized according to a target staining base to obtain a standardized image; step 4, cross-modal anchor point lists are established by manually comparing cytological and histological images; step 5, a hierarchical label set is generated by synthesizing multi-level unique labels according to the severity full sequence; step 6, the intersection-over-union index and the kappa consistency value are calculated according to the combination key, and a review to-do list is generated; step 7, point review and rule revision are carried out, and a data dictionary, a consistency report and an anchor point snapshot are output. The application realizes the standardized database construction and traceable quality control of cervical cancer cytological and histological pathological images.

The invention provides a tumor infiltration capacity enhanced TIL and a preparation method and application thereof, and belongs to the technical field of cells. The tumor infiltration capacity enhanced TIL provided by the invention is a TIL which enables the expression of the DGKZ gene to be reduced or the function to be eliminated, and has enhanced tumor infiltration capacity compared with the TIL without the DGKZ gene knockout. The tumor infiltration ability enhanced TIL provided by the invention is higher in survival rate in a depletion induction experiment, and shows depletion antagonism characteristics; experiments for performing stimulation culture on the TIL cells by using PMA show that the cytokine secretion ability is remarkably enhanced compared with that of wild TIL cells. A mouse in-vivo experiment shows that compared with a wild TIL, the DGKZ-edited cervical cancer-derived TIL cell has the advantages that the in-vivo tumor infiltration capacity and retention time are more lasting, the tumor killing capacity is higher, a better treatment effect can be achieved, and the DGKZ-edited cervical cancer-derived TIL cell has an important application value in tumor treatment.

The utility model discloses a kind of for cervical cancer's rear-mounted precision close-range treatment device, belong to the technical field of treatment equipment, including positioning plate, the positioning plate is equipped with through-hole, guiding column is installed in the through-hole, the inside both sides of the positioning plate are provided with adjusting hug device, the adjusting hug device passes through through-hole side wall, from both sides hug guiding column, the guiding column one end is provided with limit plate, the limit plate is located in the positioning plate side, the guiding column is equipped with central hole and several groups of side hole, central insertion pipe is installed in the central hole, side insertion pipe is installed in the side hole, the utility model discloses a kind of adapt to different patients use, improve the positioning effect of insertion needle, reduce the treatment device of influence to radiotherapy effect.