383 results about "Chain reaction" patented technology

The invention discloses application of iron ruthenium nano-enzyme in preparation of nucleic acid detection products, and belongs to the technical field of biological detection and nano-materials. The RIN has stable peroxidase-like (POD) activity, and can catalyze a chromogenic reaction between 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and hydrogen peroxide (H2O2). The catalytic activity of the nano-enzyme can be selectively inhibited by pyrophosphate (PPi) generated in a nucleic acid amplification reaction, so that visual colorimetric analysis on whether target nucleic acid exists or not is realized. In combination with a polymerase chain reaction (PCR) system, the invention constructs a new nucleic acid detection strategy which does not need fluorescence labeling, is simple and convenient to operate and has high sensitivity. Through verification, the system can be widely applied to rapid on-site screening and visual interpretation of related nucleic acid of drug-resistant pathogens (such as ESKAPE flora) and the like, and has a good application prospect.

The invention discloses an industrial equipment linkage control system based on dynamic time sequence reconstruction, relates to the technical field of kiln linkage control, solves the technical problems that a multi-system collaborative dynamic time sequence reconstruction capability is lacked and chain reaction caused by disturbance coupling is difficult to solve, and provides an industrial equipment linkage control system based on dynamic time sequence reconstruction by distinguishing humidity abnormal levels and customizing a heating strategy. The method ensures that heat release is completely matched with a wet material transmission rhythm, improves product drying uniformity, accurately calculates retention time and rotation speed according to a kiln type, realizes a'moisture evaporation demand-rotation speed-retention time 'closed loop, reduces energy consumption, integrates real-time material parameters, waste heat recovery and environment heat dissipation, accurately calculates a heat gap, and performs graded response, so that the energy consumption is reduced, and the energy consumption is reduced. And finally, a cooling air volume-pressure correlation model and an induced draft fan compensation-pressure repair model are established, accurate pressure compensation during cooling strengthening is achieved, and the stability of the microenvironment in the kiln is improved.

The invention relates to the technical field of aluminum oxide production, and provides an evaporation station intelligent optimization system based on multivariable coupling regulation, which comprises a DCS (Distributed Control System) lifting module used for optimizing a control strategy and hardware support; the intelligent optimization control module is used for realizing multivariable coupling regulation and control; the data storage and analysis module is used for historical data recording and process analysis; the execution module is used for receiving the control instruction and driving field equipment; the DCS control system lifting module, the intelligent optimization control module and the data storage and analysis module are in bidirectional communication connection in sequence, and the intelligent optimization control module is in unidirectional communication connection with the execution module. Through multivariable coupling regulation and an intelligent algorithm, mother liquor discharging index fluctuation is stabilized, and the operation qualification rate and the alkali circulation efficiency are improved; steam consumption matching is optimized, and energy conservation and consumption reduction are achieved; variable fluctuation chain reaction is reduced, and abnormal response time is shortened; automation of key procedures is promoted, and manual errors are reduced.

The invention provides a method for synchronously inverting unsaturated soil solute reaction parameters, and belongs to the technical field of software development. The method comprises the following steps: establishing an initial model for simulating chain reaction migration of solutes in unsaturated soil based on HYDRUS-1D software, and generating a corresponding input file; based on observation data of the one-dimensional vertical infiltration test, determining a plurality of solute reaction parameters to be synchronously inverted and an initial value range thereof; adopting a genetic algorithm to randomly generate an initial population containing multiple groups of parameter combinations in the initial value range; performing solute transport simulation on each group of parameter combination in the population, and automatically extracting a simulation result; calculating a fitness value corresponding to each parameter combination based on the observation data and the simulation result obtained in the step S4; and iteratively optimizing, and judging whether a termination condition of the genetic algorithm is met or not. By optimizing a software running program and adjusting automatic extraction and processing of calculation parameters, efficient and continuous soil solute transport analysis is realized.

The present document describes methods and systems for amplifying and quantifying amplification of a nucleic acid molecule, with a polymerase chain reaction (PCR) or a loop-mediated isothermal amplification (LAMP), by irradiating, with a heating activation light beam from a continuous wave laser a biological enzymatic reaction mixture in solution comprising a nucleic acid template, a polymerase enzyme, and chemically modified nanoparticles. Quantification of the amplification is achieved by irradiating the biological enzymatic reaction mixture during an annealing and / or elongation steps with an ultraviolet (UV) light source and measuring with a photodetector a transmission change in UV light transmission.

The invention discloses a preparation method of reactive antioxidant grafted modified nylon 6 and a film thereof, and belongs to the technical field of nylon modification. A reactive antioxidant containing a hindered phenol structure and nylon 6 are subjected to melt chemical grafting to form a covalent bond combined nylon 6-antioxidant system, and hindered phenol provides hydrogen atoms and can be combined with free radicals generated in the aging process, so that the chain reaction of the hindered phenol is terminated, and phenoxy free radicals with low activity are formed by the hindered phenol. According to the invention, the reactive antioxidant grafted modified nylon 6 is further processed into a film product, and the nylon 6 film product prepared by the method has the advantages of less antioxidant migration, aging resistance, high mechanical property retention rate and the like.

The invention relates to an organic combined polluted underground water in-situ remediation method based on a micro-nano iron-based material. The method comprises the following steps: acquiring underground water pollution information; establishing a composite reaction zone at a preset position, and constructing a reduction-oxidation chain reaction system; and operating the composite reaction zone, collecting monitoring data, and optimizing the composite reaction zone until the composite pollution is degraded and removed to a preset value. Through series reaction of powerful reduction of the micro-nano iron-based material and advanced oxidation of the iron-based activated persulfate, the performance of the micro-nano iron-based material serving as a strong reduction material and an active agent is fully played, pollutants such as halogenated hydrocarbon in underground water are sequentially subjected to reduction degradation, pollutants such as benzene series and volatile phenol are sequentially subjected to oxidative degradation, and the pollution to the underground water is reduced. Effective management and control of underground water combined pollution of medical and chemical parks and enterprises are realized; a monitoring well is configured based on a composite reaction zone, and remediation materials are discontinuously added in combination with management and control conditions, or other management and control materials are flexibly selected, so that long-term management and control of groundwater pollution risks are realized.

The invention relates to an automatic pre-warning system after equipment failure for a smart power plant. The automatic pre-warning system comprises a data acquisition unit, a fault point judgment unit, a fault propagation relationship network establishment unit, a shutdown risk assessment unit and a pre-warning unit. The method has the beneficial effects that through cooperative work of data acquisition, fault point judgment, fault propagation relationship network modeling, shutdown risk assessment and an early warning unit, fault propagation chain reaction is dynamically quantified, and the shutdown risk probability of the whole plant is generated, so that the defects of incomplete path analysis and lagging risk calculation in a multi-equipment fault scene in a traditional method are overcome; the accuracy of fault early warning is improved, so that an effective emergency response basis is provided for operation and maintenance personnel of an intelligent power plant; the propagation probability matrix generation module corrects the final propagation probability according to the actual fault propagation mark value, and the final propagation probability is corrected in each fault propagation event, so that prediction becomes more and more accurate along with system operation.

The invention discloses a flame-retardant PA66 material and a preparation method thereof.The flame-retardant PA66 material is prepared from, by weight, 80-100 parts of a modified PA66 material, 3-5 parts of modified graphene, 15-30 parts of a flame-retardant additive and 0.1-0.5 part of tetraphenylphosphorus bromide, a molecular chain of the flame-retardant additive contains an organic silicon chain segment and an organic phosphorus structure and is doped with nitrogen and boron elements, and when the material is burnt, the flame-retardant PA66 material has a flame-retardant effect, and the flame-retardant PA66 material has a flame-retardant effect. The organic phosphorus can be dehydrated to form a phosphoric acid carbon layer, the nitrogen element can release inert gas, dilute combustible gas and generate an expansive phosphorus-nitrogen carbon layer with the organic phosphorus, the boron element can improve the compactness and high-temperature stability of the carbon layer, and the organic silicon can form a cross-linked network shape, so that the continuity of the carbon layer is improved; a fluorine-containing polysiloxane chain segment of a side chain of the flame-retardant additive is embedded among molecules of the modified PA66 material, and is decomposed to generate fluorine free radicals during combustion, and active free radicals in a combustion chain reaction are quenched, so that a rapid flame-retardant effect is achieved.

The invention relates to the technical field of chip interconnection, in particular to a virtual fault domain isolation and recovery method suitable for a Lingqu interconnection system, which comprises the following steps: when a fault event occurs, a state machine is switched to a QUIESCE mode, and a transaction shadow table is maintained; carrying out classification processing on the uncompleted transactions in the transaction shadow table, and monitoring the state of the access object; and after reconnection, task replay is carried out according to the transaction shadow table, and the state machine is switched back to the ACTIVE state. In order to solve the problem that in the prior art, a third-party node with a fault in a Lingqu interconnection system is prone to causing chain reaction, an isolation mechanism is added to an IO interconnection chip used for being connected to a third-party chip, a state machine of a fault domain is switched to a QUIESCE mode, a transaction shadow table of an uncompleted transaction is established, and the transaction shadow table of the uncompleted transaction is obtained. According to the method and the system, the IO interconnection chip performs proxy processing on part of transactions after the access object is recovered, so that faults of other equipment in the interconnection system are prevented from being caused in a fault reconnection stage, and replaying is performed after the access object is recovered, thereby realizing risk isolation and recovery processes.

The invention relates to the technical field of refrigeration air conditioners, in particular to an efficient environment-friendly low-GWP air conditioner refrigerant formula and a preparation method thereof. The technical problem that an existing low-GWP refrigerant is high in flammability is solved. According to the refrigerant formula prepared by the invention, a high-energy-efficiency natural working medium and an ultralow-GWP fourth-generation refrigerant are taken as main components, a small amount of phosphorus-nitrogen hybrid compound is added as a flame-retardant additive, and the main components are mixed under the conditions of low temperature and high pressure. The flame-retardant additive adopted by the invention has excellent flame retardance, and can capture free radicals and release inert gas to inhibit combustion chain reaction during combustion. Nanoscale dispersion of the additive is realized through a low-temperature ultrasonic-assisted microjet homogenization technology, and the stability of the components is ensured by utilizing online monitoring. According to the refrigerant prepared by the invention, the combustibility is greatly reduced while the refrigeration performance is ensured, and both low GWP and low combustibility are realized.

The embodiment of the invention discloses a preparation method of a kit applied to endometrial cancer microsatellite state detection. The preparation method comprises the following steps: taking a first preset volume of polymerase chain reaction amplification liquid; taking a polymerase chain reaction primer mixed solution with a second preset volume; and combining the weighed polymerase chain reaction amplification liquid and polymerase chain reaction primer mixed liquid into the kit applied to endometrial cancer microsatellite state detection. According to the embodiment, the accuracy and the stability of microsatellite state detection of the endometrial cancer can be improved, so that the kit consumed by repeatedly detecting the microsatellite state in the endometrial cancer is reduced.

The invention discloses a silicon carbide substrate rough polishing composition and a preparation method and application thereof, and belongs to the technical field of semiconductor device processing. The rough polishing composition for the silicon carbide substrate is a polishing composition obtained by adjusting the pH value of a basic system to 3-4 by using a pH regulator; the basic system comprises the following components in percentage by mass: 2-10% of an abrasive, 2-6% of a first oxidant, 2-10% of a second oxidant and 0.5-2% of a dispersing agent besides water; the first oxidant is potassium permanganate; the second oxidizing agent is potassium persulfate or ferric persulfate. According to the method, the combination of potassium permanganate and persulfate is used as an oxidizing agent, and the rough polishing efficiency and uniformity of silicon carbide are remarkably improved through oxidation potential complementation, acidic environment synergy, free radical catalysis chain reaction and surface wettability optimization.

The PCR plate according to the present invention includes: a body portion having one or more reaction wells; an insertion portion extending from the body portion, inserted into a nucleic acid extraction cartridge, and having an injection port into which a nucleic acid solution is injected; a flow path portion allowing the nucleic acid solution to flow from the injection port to the reaction wells; and a blocking portion attached to the body portion for blocking backflow of the nucleic acid solution from the reaction wells to the flow path portion side.

The invention discloses a self-repairing bio-based coating material giving consideration to nutrient controlled release and membrane shell degradation and a preparation method of the self-repairing bio-based coating material, and belongs to the technical field of controlled release fertilizer production. The self-repairing bio-based coating material is prepared from the following raw materials in parts by weight: 80 to 100 parts of easily degradable polyester polyol, 30 to 50 parts of cross-linking promoting agent and 10 to 20 parts of functional chain extender. According to the self-repairing bio-based coating material and the preparation method thereof, the easily degradable polyester polyol reacts with the cross-linking promoting agent and the functional chain extender to synthesize the self-repairing bio-based coating material which gives consideration to nutrient controlled release and membrane shell degradation, and the self-repairing bio-based coating material can improve the toughness of a membrane material, promote rapid repairing of a damaged area and improve the service life of the membrane material. The stability of nutrient release is improved; in addition, a chain reaction of structural fracture can be triggered in the membrane material degradation process, and the degradation rate of a membrane shell after nutrients are completely released is increased. The invention effectively solves the problem that the degradation performance of the membrane shell and the controlled release performance of nutrients are difficult to coexist.

The invention provides a ready-to-use PCR tube preloaded with Taq polymerase and reaction components and stable at room temperature, and belongs to the field of molecular biology and nucleic acid diagnosis. The invention provides a ready-to-use PCR (polymerase chain reaction) tube which can be stored at room temperature and is pre-filled with a premix for carrying out polymerase chain reaction (PCR) amplification on all necessary components in advance. The kit is characterized in that each tube is pre-filled with locally prepared Taq polymerase, a PCR buffer solution, dNTPs, MgCl and target specific primers (only a customized PCR kit-ready-to-use PCR plus tube contains the primers), and all the components are dried and stored in a dry powder form, so that the shelf life can be prolonged, and the tedious steps of on-ice operation can be avoided. According to the method, the error rate in the PCR preparation process is reduced to the maximum extent, and the diagnosis and research work flow is simplified.

A fiber optic device and method for uniform illumination of biological samples distributed over one or more microcell arrays of a microwell plate can establish more reliable polymerase chain reaction (PCR) data sampling. In some embodiments, the apparatus includes a light engine that emits a light beam captured by a fiber optic bundle for delivering one or more light beams to each microchamber; each light beam thereby fluoresces the biological sample. The fiber optic cable of the fiber optic bundle comprises a distal output end held in place using a mechanical support for flexibly aligning the output beam of the distal output end to be arranged such that the center of the output beam is directed to different locations in the one or more microcell arrays, wherein each of adjacent ones of the output beams partially overlaps so that the light distribution is uniform.

The invention relates to a system for amplifying nucleic acids, in particular by means of a polymerase chain reaction, comprising a fluid receptacle (1), in particular a microtiter plate or a microchip, and a reaction mixture (2), which contains at least one nucleic acid (2.1) to be amplified, a polymerase enzyme, a buffer medium (2.2), a plurality of excitable nanoparticles (3), preferably nanostars or nanorods, and quantification means, in particular a fluorescent and / or intercalating nucleic acid dye, the reaction mixture (2) being receivable in the fluid receptacle (1). The system further comprises a reaction chamber (4), which has excitation means, the fluid receptacle (1) preferably being introducible into the reaction chamber (4) and the excitation means (5) being designed to excite the nanoparticles (3); the nanoparticles (3) are designed to generate heat in the excited state; and the reaction chamber (4) has a detection device (6), which is designed to excite the quantification means and quantitatively and / or qualitatively detect same, said excitation means being near-infrared radiation emitters (5).

A method for quantification of a target molecule of a biological sample distributed across a plurality of partitions is provided. The method includes receiving fluorescent emission data generated by a polymerase chain reaction (PCR) for each partition of a set of partitions and identifying a partition quantity metric for each partition in the set of partitions to generate a set of partition quality metrics. Each partition quantity metric correlates to a number of target molecules within its respective partition based on the fluorescent emission data. The set of partition quantity metrics is used to determine a state of target molecule amplification within each partition of the set of partitions. The method further includes determining a quantity of the target molecule in the biological sample based on the set of partition quantity metrics.

The present invention relates to a method for separating and enriching a chlorine-37 isotope using a laser photochemical reaction and, specifically, to a method for separating and enriching a high-purity chlorine-37 isotope, which is essential for a next-generation molten chloride fast reactor (MCFR) and the like, by selectively photolyzing specific isotopic molecules of iodine monochloride (ICl) using a high-power fiber laser or a solid-state Raman laser and reacting same with a radical scavenger to effectively terminate a chain reaction. Accordingly, by employing, as a light source, a single-frequency fiber laser or a solid-state Raman laser that has higher power efficiency and easier maintenance than conventional dye lasers and is capable of achieving high power of several kW or more through modular expansion, mass production on an industrial scale can be realized and the economic feasibility of the process can be significantly secured.

The present application relates to the technical field of analytical detection, and in particular to a detection method of Geobacillus thermophilus in raw milk based on real-time fluorescent quantitative polymerase chain reaction. The detection method of the present application is based on nucleic acid specific detection mechanism, which replaces the phenotype culture mechanism, realizes the rapid and accurate quantification of the target bacteria, and replaces the time-consuming and inaccurate colony counting. Through the design of specific primers and probes, the species level specificity identification is realized, and the problem that the traditional method cannot distinguish the specific contaminated bacteria is solved. Through the optimization of the pretreatment method, the sensitivity of the detection method is greatly improved. The detection method of the present application shortens the detection time, realizes the leap from "days" to "hours", greatly improves the detection efficiency, and has a wide application prospect.

This application provides a thermal runaway guidance system based on alternating positive and negative electrode arrangement and directional pressure relief, comprising: an outer isolator with a honeycomb-structured grid plate at one end, and a plurality of heat dissipation holes evenly distributed on both side walls of the outer isolator; an inner isolator disposed inside the outer isolator; a fire extinguishing box disposed inside the inner isolator, and a plurality of battery cells arranged inside the fire extinguishing box; the battery cells are arranged in an alternating positive and negative electrode staggered manner, the pressure relief valves of each battery cell face the same direction, and adjacent battery cells are connected by fuses; a heat-sensitive fire extinguishing mechanism is provided at the positive electrode connection of at least one battery cell; the battery cells are disposed in the mounting holes of the mounting bracket, and a plurality of evenly distributed pressure relief holes are provided at the end of the inner isolator near the grid plate. This system avoids the chain reaction caused by rapid heat transfer between battery cells during thermal runaway; at the same time, it ensures that the pressure relief direction is consistent, and the high-temperature and high-pressure gas is released regularly, preventing the spread of fire and preventing impact damage to surrounding structures.

The application discloses MS glue, an MS glue additive and a preparation method of the MS glue additive, and the preparation method of the MS glue additive comprises the following steps: dissolving pentamethyl piperidyl alcohol in an organic solvent, heating, and stirring; adding octadecadiene acid dimer, and stirring; adding diisopropyl bis(dioctyl pyrophosphoric acyloxy) titanate, and stirring; heating, and extracting the solvent under reduced pressure to obtain a product. In the technical scheme, the MS glue additive can effectively capture free radicals in a polymer degradation chain reaction, thereby inhibiting a photo-oxidation process, and achieving a light stabilization purpose. Through evaluation, the additive can greatly reduce performance attenuation and yellowing of the MS glue under light aging.

Provided is an accurate guide RNA (gRNA) screening method for base editing of an asialoglycoprotein receptor 1 (ASGR1) gene, including the following steps: (1) gRNA design; (2) primer design; (3) in vitro transcription of gRNA; (4) cell transfection; (5) collection of cells, and extraction and polymerase chain reaction (PCR) of a genome; and (6) Sanger sequencing.

Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite fluorescence signal data associated with a composite fluorescence signal from at least a first probe type comprising a first fluorophore and a second probe type comprising a second fluorophore which has substantially overlapping spectral characteristics as said first fluorophore, the first probe type and the second probe type differing in thermal and / or temporal properties; and determining, based at least partially on the composite fluorescence signal data, fluorescence signal data associated with a fluorescence signal from a given probe type of the first probe type or the second probe type during the amplification process.

The present invention relates to a method for simultaneously detecting a plurality of pathogens from biologically-derived samples, and a kit for carrying out the method. Specifically, the present invention relates to a method for simultaneously detecting a plurality of pathogens that cause infectious uveitis, one of eye infections from samples such as anterior chamber fluid or vitreous by polymerase chain reaction (PCR), and a kit for carrying out the method.

The application provides a nucleic acid sequence and a method for detecting biomolecules based on a self-driven hybrid chain reaction (SHCR), which comprises a priming chain AI, a blocking chain B, a hairpin probe H1, a hairpin probe H2, a hairpin probe H3 and a hairpin probe H4, the priming chain AI comprises an aptamer sequence of a to-be-detected biomolecule and a starting sequence I, the sequence of the starting sequence I is a*‑b*, wherein a* and b* are gene fragments; the 5' end of the hairpin probe H1 is connected with b*, and the 3' end of the hairpin probe H3 is connected with a*. The application further provides a kit for detecting biomolecules based on the SHCR and a method for detecting biomolecules, in particular, a method for detecting a kanamycin residual amount in food. Through ingenious design, when the to-be-detected biomolecule exists, the sequence combination of the above biomolecule can initiate the self-driven hybrid chain reaction, the priming chain can be autonomously supplemented, the self-driven reaction process is reversely activated, thereby a more significant FRET signal is generated, and the detection sensitivity is improved.

Safe addressable firing controls positioned within a reusable housing that only allows voltage and current from the shooting panel to reach the explosive device by addressing the switch to open allowing electricity to pass through it and setting off the chain reaction during detonation, wherein the safe addressable firing controls positioned within a cartridge that creates a safety barrier between the surface equipment and a downhole tool, such as an explosive device.

This application provides a roadside system based on the HarmonyOS operating system. The roadside system includes a transaction system and multiple security subsystems. The transaction system is developed based on a standard HarmonyOS system, and each basic unit within the transaction system can be installed, run, uninstalled, and upgraded independently. Each security subsystem is developed based on a lightweight HarmonyOS system and operates independently. The transaction system creates multiple transaction threads. Each transaction thread sends vehicle information from the corresponding vehicle terminal to the corresponding security subsystem for security authentication. It receives the authentication result from the vehicle terminal corresponding to each transaction thread and completes the transaction with the successfully authenticated vehicle terminal. This application solves the problem of chain reactions caused by the failure of a single functional module due to severe coupling between the various functions of the roadside system.

An apparatus for amplifying segments of nucleic acid in a mixture sample via polymerase chain reaction (PCR) has a body including a heating element and a Mixture sample conduit. The heating element includes a denaturation zone, an extension zone and an annealing zone arranged on the body The Mixture sample conduit includes an inlet, an outlet and an intermediate section that passes serially through the denaturation zone, the annealing zone and the extension zone for multiple cycles. A related method for amplifying segments of nucleic acid in a mixture sample via polymerase chain reaction is also described.