The invention discloses an SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect gene points, namely qFS-1-1, qFS-1-2, qFS-1-3, qFS-1-4, qFS-1-5, qFS-9-1, qFS-9-2 and qFS-2-1, wherein qFS-1-1, qFS-1-2, qFS-1-3, qFS-1-4 and qFS-1-5 are positioned on c25 chromosomes, qFS-9-1 and qFS-9-2 are positioned on c7 chromosomes, qFS-2-1 is positioned on a c13 chromosome, the marker interlocked with the qFS-1-1 is Gh478350, the marker interlocked with the qFS-1-2 is DPL0387220, HAU892170 and DC40429190, the marker interlocked withthe qFS-1-3 is COT012140, the marker interlocked with the qFS-1-4 is SHIN-1131240, PGML00463450, Gh537190 and PGML00463250, the marker interlocked with the qFS-1-5 is HAU916150, CGR5201200 and CGR564385, the marker interlocked with the qFS-9-1 is CGR5001245, the marker interlocked with the qFS-9-2 is HAU1693150, and the marker interlocked with the qFS-2-1 is SHIN-1452115 and PGML00353240. By utilizing the SSR markers interlocked with the cotton high-strength fibre major-effect genes to carry out auxiliary selection, the efficiency of the cotton fibre quality breeding can be greatly improved.