Patents
Literature
Patsnap Copilot is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Patsnap Copilot

98 results about "Single sequence" patented technology

Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing

InactiveUS6418382B2Rapid and economical and quantitative and precise determination and classificationSufficient discrimination and resolutionData processing applicationsMicrobiological testing/measurementSample sequenceSingle sequence
This invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification. Another embodiment of the invention uses information on the presence or absence of carefully chosen target subsequences in a single sequence clone together with DNA sequence databases to determine the clone sequence. Computer implemented methods are provided to analyze the experimental results and to determine the sample sequences in question and to carefully choose target subsequences in order that experiments yield a maximum amount of information.
Owner:CURAGEN CORP

Method and apparatus for providing atrial autocapture in a dynamic atrial overdrive pacing system for use in an implantable cardiac stimulation device

Techniques for providing capture verification during overdrive pacing are described. If an overdrive pacing pulse fails to evoke capture (i.e. a loss of capture occurs), a high voltage backup pulse is automatically delivered. Once a second loss of capture occurs during a single sequence of overdrive pacing pulses, an overdrive pulse capture threshold detection search, described herein, is performed while overdrive pacing continues. Various techniques for providing rate recovery are also described herein. The rate recovery techniques are designed to avoid problems that might arise from possible fusion of intrinsic beats and overdrive pacing pulses that fail to evoke capture. In a first rate recovery technique, capture detection is suspended during rate recovery due to the possibility of fusion. Instead, an extra safety margin is added to the overdrive pulses. Once two intrinsic beats are detected, automatic capture verification is reactivated for the next two beats to verify capture before the new overdrive rate is finally established. In a second rate recovery technique, capture verification is maintained throughout rate recovery but the pulse magnitude is increased to a high output mode voltage to avoid any risks of fusion. After the output is increased to the high output mode voltage, a subsequent loss of capture is considered to be an intrinsic event for the purposes of terminating rate recovery. In a third rate recovery technique, capture verification is maintained during rate recovery and the output energy is not increased to the high output mode voltage unless loss of capture is first detected. Subsequent loss of capture events are then counted as P-waves.
Owner:PACESETTER INC

Method for constructing wheat SSR (single sequence repeat) fingerprint

The invention discloses a method for constructing a wheat SSR fingerprint, which belongs to the field of agricultural wheat breeding and application technologies. The method comprises the following steps: extracting DNA (deoxyribonucleic acid) of a wheat variety to be tested; carrying out PCR (polymerase chain reaction) amplification to the DNA Of the wheat variety to be tested by using 13 pairs of basic core SSR fluorescent dye primers with the ID No.1 to 26 and shown in a sequence table SEQ and 12 pairs of extended core SSR fluorescent dye primers with the ID No.27 to 50 and shown in the sequence table SEQ respectively; and detecting PCR amplification products by denaturing polyacrylamide gel electrophoresis or five-color fluorescent capillary electrophoresis, and constructing the SSR fingerprint of the wheat variety. The method provided by the invention can identify the wheat variety in a laboratory by using technologies of the PCR amplification, the gel electrophoresis and capillary electrophoresis according to the polymorphism of SSR sites, can greatly improve the test efficiency in time, and has the advantages of high speed, high accuracy, convenience for operation and the like; and the identification result is not liable to be affected by the environment.
Owner:CROP RES INST SHANDONG ACAD OF AGRI SCI

Sequence generating method and device

The invention provides a sequence generating method and device. The method comprises the following steps: receiving a sequence generating request sent by a client, wherein the sequence generating request carries an identification from the client; generating a deviation segment character string according to the identification from the client, and searching a corresponding deviation segment according to the deviation segment character string, wherein the deviation segment comprises a deviation value and a sequence pool identification; searching a sequence pool indicated by the sequence pool identification, regarding the deviation value as an index of the sequence pool to position to a corresponding sequence number in the sequence pool, sending the sequence number to the client, and adding one for the deviation value to obtain a new deviation value. The used sequence numbers are in the same sequence pool for the same client, the single sequence number is acquired by the client request by use of a business identification, thereby guaranteeing the uniqueness and orderness of the overall sequence; multiple clients are simultaneously erupted without influencing each other, the sequence acquisition performance is improved, and the problems of congestion and bad performance under the high concurrence scene in the prior art are solved.
Owner:HUAWEI TECH CO LTD

Kit and device for detecting aneuploidy of chromosomes

The invention discloses a kit and device for detecting aneuploidy of chromosomes. The device comprises a sequenced data detection module, a first coverage calculation module, a ZCNV value calculation module, a copy number variation fragment query module, a copy number variation fragment determination module, a first alpha calculation module, a second alpha calculation module, a correction module, a second coverage calculation module, a Zaneu value judgment module and a chromosome aneuploidy confirmation module, wherein the sequenced data detection module is used for obtaining sequenced data for all the chromosomes; the first coverage calculation module is used for obtaining uncorrected coverage of each chromosome; the ZCNV value calculation module is used for calculating a ZCNV value of a single sequence number of each window; the copy number variation fragment query module is used for querying fragments, of which the ZCNV value of over 80% of windows is greater than or equal to 4 or smaller than or equal to -4, with over 300Kb in the sequenced data; the copy number variation fragment determination module is used for determining copy number variation fragments of a pregnant woman to be detected; the first alpha calculation module is used for calculating a parameter alpha according to a formula (1); the second alpha calculation module is used for calculating the parameter alpha according to a formula (2); the correction module is used for correcting the uncorrected coverage so as to obtain corrected coverage; the second coverage calculation module is used for calculating a Zaneu value of each chromosome; the Zaneu value judgment module is used for judging whether the Zaneu value is not smaller than 3 or not; the chromosome aneuploidy confirmation module is used for determining that the chromosomes have aneuploidy. The detection is more accurate.
Owner:ANNOROAD GENE TECH BEIJING +3

Joint prediction method of base station traffic

The invention provides a joint prediction method of base station traffic. The problem that the traditional linear algorithm is bad in prediction performance when the traffic data is nonlinear and hasa sudden change value is solved. The method comprises the following steps: firstly collecting traffic data from a base station as a data set, performing data preprocessing on an abnormal value and a missing value; decomposing processed data by adopting wavelet transform, enabling the traffic data to be smooth and easy to predict; performing single reconstruction on a sequence obtained through decomposition, wherein a low-frequency signal is predicted by adopting an echo state network model, and a high-frequency signal performs prediction by adopting an autoregression integral sliding average model; and finally performing linear accumulation on the prediction numerical value of the single sequence to obtain a final result. Compared with the single model prediction, the joint model method disclosed by the invention can reach better prediction, the reduced average absolute percentage error can achieve 6%, and the normalization root mean square error is reduced to a certain degree; the traffic data prediction accuracy of the base station is improved, and the network resource reasonable allocation can be improved.
Owner:NANJING UNIV OF POSTS & TELECOMM

Bearing retainer automatic processing method

The invention discloses a bearing retainer automatic processing method. The bearing retainer automatic processing method includes following steps: feeding work pieces to be processed into a sorting machine so as to sieve and arrange the work pieces to be processed, and forming a rank by keeping the work pieces at the same open direction and arranging the work pieces in single sequence; separately feeding all the work pieces to be processed into a hoister one by one, and hoisting the work pieces to be processed to a position as high as a machine tool; dropping the work pieces to be processed into an arc groove, and using a thruster to push the arc groove to a designated processing position; using an air cylinder to drive an air cylinder arm to tightly press a transmission shaft; using a motor to drive a belt pulley to rotate, and thereby driving the transmission shaft to rotate, and further rotating the work pieces to be processed; using a stepping motor to adjust the length of a threaded shaft of the stepping motor, and then performing fine adjustment on positional accuracy of a cutting tool; using the cutting tool to cut the work pieces to be processed. The bearing retainer automatic processing method can efficiently and rapidly fix and rotate the work pieces to be processed, uses the cutting tool fixed in position to process the semi-finished work pieces, achieves the purpose of replacing manual cutting with automatic operation, achieves continuous processing, decreases the number of operation procedures, and improves production efficiency.
Owner:QUZHOU FANGONG ELECTRICAL TECH

SFC-to-ladder-diagram conversion method based on PLC programming

The invention discloses an SFC-to-ladder-diagram conversion method based on PLC programming. The method includes the following steps: (1) performing traversal on an SFC program column link list so as to obtain program blocks containing effective icons and converting the program blocks into a two-dimensional array for storage; (2) dividing the program blocks in step (1) into single-sequence program blocks and serial and parallel connection program blocks and if the program blocks are single-sequence program blocks, converting the program blocks directly into ladder diagrams, or else entering step (3); (3) judging whether the serial and parallel connection program blocks in step (2) are of a serial-connection structure or a parallel-connection structure; (4) dividing the serial and parallel connection program blocks in step (2) into a plurality of branches and checking each branch; if the branches are of a single-sequence structure, directly converting the branches into a ladder diagram, or else entering step (2). The invention provides the SFC-to-ladder-diagram conversion method so that a work of converting an SFC into the ladder diagram and then further converting the ladder diagram into an instruction table is completed so that communication of the PLC and a lower computer is realized.
Owner:SOUTHEAST UNIV

Human motion identification method based on second generation Bandelet statistical characteristics

The invention discloses a human motion identification method based on second generation Bandelet statistical characteristics, which mainly solves the problems of complicated characteristic extraction and poor representational capacity in the prior art. The human motion identification method comprises the steps of: 1, converting video in a Weizmann database into a sequence image, constructing a training sample set X and a test sample set T according to a proportion of 8:1; 2, carrying out second generation Bandelet transformation on a single sequence image in the sample sets, sequentially extracting an energy characteristic Ve, an entropy characteristic Vs, a maximum value characteristic Vmax, a minimum value characteristic Vmin, a contrast ratio characteristic Vc, a mean value characteristic Vmu and a variance characteristic Vv of the image, and cascading all the characteristics to be used as final characteristics of the single image; and 3, repeating the step 2 for respectively extracting characteristics of all sequence images in the training sample set X and the test sample set T to obtain a training sample characteristic set X* and a test sample characteristic set T*, and carrying out learning training on the training sample characteristic set X* and the test sample characteristic set T* by using an Adaboost algorithm to obtain a classifying result. The human motion identification method can be used for accurately identifying a human motion, and can be used in video monitoring and video processing of target identification.
Owner:XIDIAN UNIV

Material sheet stamping device

InactiveCN103008443AAchieve dimensional accuracySolve efficiency problemsPerforating toolsPunchingEconomic benefits
The invention discloses a material sheet stamping device and relates to the technical field of machining. The material sheet stamping device comprises a base; a front bracket is arranged on the base; the base is also provided with at least one group of punching die block; one side of each group of punching die block is respectively provided with a discharge frame; each group of punching die block comprises a lower die seat and an upper die seat; each lower die seat is respectively connected with a lower cutter; each upper die seat is respectively arranged above the corresponding lower die seat by a spring assembly; each upper die seat is respectively connected with an upper cutter and a stripper plate; each stripper plate is respectively arranged at one side of the corresponding upper cutter; the base is also connected with a first cylinder which is vertically arranged; the first cylinder is arranged between two groups of punching die blocks; the extension and retraction end of the cylinder is connected with a supporting frame. According to the material sheet stamping device, not only can size accuracy and production efficiency of coil feed line swing shears be achieved, is production cost lower than that of a single-sequence blanking process and are the difficult problems of low production efficiency and high production cost of trapezoid materials solved, but also automation operation is implemented, operators are reduced, production cost is saved and more economic benefits are obtained.
Owner:YIZHENG SHENWEI STAMPING

SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect genes

The invention discloses an SSR (Single Sequence Repeats) marker interlocked with high-quality cotton fibre material 0-153 high-strength fibre major-effect gene points, namely qFS-1-1, qFS-1-2, qFS-1-3, qFS-1-4, qFS-1-5, qFS-9-1, qFS-9-2 and qFS-2-1, wherein qFS-1-1, qFS-1-2, qFS-1-3, qFS-1-4 and qFS-1-5 are positioned on c25 chromosomes, qFS-9-1 and qFS-9-2 are positioned on c7 chromosomes, qFS-2-1 is positioned on a c13 chromosome, the marker interlocked with the qFS-1-1 is Gh478350, the marker interlocked with the qFS-1-2 is DPL0387220, HAU892170 and DC40429190, the marker interlocked withthe qFS-1-3 is COT012140, the marker interlocked with the qFS-1-4 is SHIN-1131240, PGML00463450, Gh537190 and PGML00463250, the marker interlocked with the qFS-1-5 is HAU916150, CGR5201200 and CGR564385, the marker interlocked with the qFS-9-1 is CGR5001245, the marker interlocked with the qFS-9-2 is HAU1693150, and the marker interlocked with the qFS-2-1 is SHIN-1452115 and PGML00353240. By utilizing the SSR markers interlocked with the cotton high-strength fibre major-effect genes to carry out auxiliary selection, the efficiency of the cotton fibre quality breeding can be greatly improved.
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products