Kit and device for detecting aneuploidy of chromosomes
A technology of aneuploidy and chromosome, applied in the field of biomedicine, can solve problems such as low error rate and misjudgment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0070] In order to test the correcting effect of the correction of abnormal copy number fragments of pregnant women on the detection of chromosomal aneuploidy, this embodiment generates a group of simulated data of pregnant women based on Poisson distribution. In the simulated data, 13, Chromosomes 18 and 21 were added with quantitative copy number abnormal fragments, and the size of copy number abnormal fragments ranged from 0.5Mb to 5Mb with a step size of 0.25Mb. Then, three different concentrations of normal human DNA (5%, 10%, 15%) were mixed into the simulated data containing fragments with copy number abnormalities. The whole process is used to simulate the impact of different copy number abnormal fragment sizes on the coverage of chromosomes 13, 18 and 21 under different fetal concentrations, and to test the correction effect of pregnant women's copy number abnormal fragments on chromosome aneuploidy detection . All calculations were performed under the assumption tha...
Embodiment 2
[0075] Utilize the device for detecting chromosomal aneuploidy of the present invention to detect fetal aneuploidy in 6615 cases of pregnant women to be tested, the device comprising:
[0076] Sequencing data detection module: used to perform high-throughput sequencing on the peripheral blood cell-free DNA of 6615 pregnant women to be tested, so as to obtain the sequencing data including all chromosomes of each pregnant woman to be tested;
[0077] The first coverage calculation module: used to calculate the coverage of all chromosomes in the sequencing data of each pregnant woman to be tested in the form of divided windows, so as to obtain the coverage before correction of each chromosome of each pregnant woman to be tested;
[0078] Z CNV Value calculation module: Z for the number of single sequences in each window for each pregnant woman to be tested CNV value is calculated;
[0079] Copy number abnormal fragment query module: used to query the fragments above 300Kb in the ...
Embodiment 3
[0091] Utilize the device for detecting chromosomal aneuploidy of the present invention to detect the above-mentioned sample (sample EK01875, the age of the pregnant woman is 29 years old, and the gestational week is about 18w). The device includes:
[0092] Sequencing data detection module: used for high-throughput sequencing of peripheral blood free DNA of pregnant women to obtain sequencing data including all chromosomes;
[0093] The first coverage calculation module: used to calculate the coverage of all chromosomes in the sequencing data in the form of divided windows, so as to obtain the coverage of each chromosome before correction;
[0094] Z CNV Value calculation module: Z for the number of single sequences of pregnant women to be tested in each window CNV value is calculated;
[0095] Copy number abnormal fragment query module: used to query fragments over 300Kb in the sequencing data, and among the fragments above 300Kb, the Z of chromosome fragments in more than...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com