Detection kit of nucleic acid of klebsiella pneumoniae KPC (Klebsiella Pneumoniae Carbapenmase) type carbapenemases gene

A technology for Klebsiella pneumoniae and carbapenemase, which is applied in the directions of microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve the problem of high detection cost, achieve high sensitivity, simple operation, and is beneficial to the industry. the effect of

Inactive Publication Date: 2013-05-08
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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Problems solved by technology

[0006] 2. Agar diffusion method: In the detection of KPC enzyme-mediated drug resistance, ertapenem is more sensitive than meropenem and subampenem alone, so it seems that ertapenem is the best method for detecting KPC-producing Enterobacter spp. optimal molecule; however, since ertapenem resistance itself is not a marker of KPC expression, as other factors such as ESBL and AmpC can also cause ertapenem resistance, such approaches are also highly limited
At present, several PCR-based and real-time detection technologies are used for the detection of KPC-producing strains. These technologies have higher specificity, but require professional technicians and equipment to implement, and the detection cost is relatively high.

Method used

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  • Detection kit of nucleic acid of klebsiella pneumoniae KPC (Klebsiella Pneumoniae Carbapenmase) type carbapenemases gene
  • Detection kit of nucleic acid of klebsiella pneumoniae KPC (Klebsiella Pneumoniae Carbapenmase) type carbapenemases gene
  • Detection kit of nucleic acid of klebsiella pneumoniae KPC (Klebsiella Pneumoniae Carbapenmase) type carbapenemases gene

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Embodiment Construction

[0033] The following examples are intended to illustrate rather than limit the invention.

[0034] blaKPC Gene Nucleic Acid Detection Kit Detection Method

[0035] 1. Collection of samples

[0036] a) Blood - collect 5-10ml whole blood, place it in an EDTA anticoagulated collection tube, seal it and send it for inspection.

[0037] b) Sputum - After repeatedly gargling with water, cough up sputum vigorously, cough up fresh sputum from the deep part of the respiratory tract, seal it in a sterile container and submit it for inspection.

[0038] c) Urine - Morning urine should be collected for routine urine testing, and women should wash their genitals with soapy water or 0.1% potassium permanganate solution before sampling. About 5-10ml of mid-section urine was collected from the front and back 1 / 3 of the urine, sealed in a sterile container and submitted for inspection.

[0039] d) Pus and wound secretions - After scrubbing the surface of the lesion with sterile normal salin...

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Abstract

The invention relates to a blaKPC (Klebsiella Pneumoniae Carbapenmase) gene detection kit, and particularly to a kit for detecting klebsiella pneumoniae KPC type carbapenemases (blaKPC) gene by utilizing a composite probe technology and a real-time fluorescence PCR (Polymerase Chain Reaction) technology. Through qualitative detection for the blaKPC gene in a sample, the kit can be widely applied to auxiliary diagnosis of bacterial infection including the blaKPC gene.

Description

technical field [0001] The invention relates to a blaKPC gene detection kit, in particular to a kit for detecting Klebsiella pneumoniae KPC-type carbapenemase gene (blaKPC gene) by using a composite probe technology real-time fluorescent PCR technology. By performing qualitative detection of the blaKPC gene in the sample, the kit can be widely used in the auxiliary diagnosis of bacterial infection containing the blaKPC gene. Background technique [0002] Klebsiella pneumoniae is one of the opportunistic pathogens causing nosocomial infection and community infection. Carbapenem antibacterial drugs are powerful weapons for the treatment of severe infections caused by Klebsiella pneumoniae, including extended-spectrum lactamase (ESBLs)-producing bacteria. However, with the extensive clinical use of carbapenem antibacterial drugs, different drug-resistant strains such as Pseudomonas aeruginosa and Acinetobacter bacteria have emerged. In 2001, since Yig it et al. discovered a n...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/22
Inventor 王升启陈苏红吕虹刘志红孙晓彦刘琦琪
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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