725 results about "Enterobacter" patented technology

A recombinant humanized collagen and a preparation method therefor. The recombinant humanized type III collagen can be efficiently and stably expressed in three expression systems of yeast, Escherichia coli, and CHO cells, and has the biological activity superior to that of a commercially available collagen product. The recombinant humanized type III collagen has structural characteristics and functional activity similar to those of a natural collagen, and thus has wide application prospects in biomedical materials, tissue engineering products, cosmetics, foods, health care products or drugs, medical instruments, medical cosmetology and the like.

The invention relates to a recombinant human XVII type collagen as well as a preparation method and application of the recombinant human XVII type collagen. The recombinant human XVII type collagen is any one of COL17A 3a, COL17A 3b, COL17A 3c, COL17A 3d or COL17A 3e. According to the invention, the functional fragments of the XVII collagen are analyzed by using computer software, the minimum functional region of the XVII collagen is screened, and expression and extraction are carried out by using an escherichia coli recombinant expression system, so that the target functional fragments with higher yield can be obtained.

The invention belongs to the technical field of microorganisms, and discloses a low-temperature-resistant aerobic denitrifying bacterium and application thereof. The low-temperature-resistant aerobic denitrifying bacterium is Enterobacter ludwigii CW-01, and the preservation number of the low-temperature-resistant aerobic denitrifying bacterium is CGMCC (China General Microbiological Culture Collection Center) No.27126. The low-temperature-resistant aerobic denitrifying bacterium can be used for preparing the The enterobacter lewis CW-01 strain disclosed by the invention can efficiently realize aerobic denitrification degradation of ammonia nitrogen, nitrate and nitrite within the temperature range of 4-35 DEG C, and is especially suitable for wastewater treatment in which the ammonia nitrogen, nitrate and nitrite are difficult to remove due to low temperature; the strain also has good salt resistance, and provides a good microbial material for biological nitrogen removal treatment of low-temperature, high-salt and high-ammonia nitrogen polluted water. The nitrogen is efficiently removed, meanwhile, the use amount of a carbon source is remarkably reduced, the enterprise cost is greatly reduced, and the method has a good application prospect in the field of sewage treatment.

The invention discloses two polypogon fugax herbicide-resistant genes and application thereof, and belongs to the technical field of herbicide-resistant genes. The invention discloses CDS sequences of PfGSTup and PfGSTl1 genes of polypogon fugax for the first time, the CDS sequence of the PfGSTup gene is as shown in SEQ ID NO. 1, and the CDS sequence of the PfGSTl1 gene is as shown in SEQ ID NO. 4; it is found for the first time that transgenic escherichia coli can obtain mesosulfuron-methyl resistance by expressing PfGSTup and PfGSTl1 genes in escherichia coli, and two new mesosulfuron-methyl resistance GST family candidate genes derived from polypogon fugax are provided; the provided PfGSTup and PfGSTl1 genes can be used for culturing ALS inhibitor herbicide-resistant wheat varieties, and two candidate genes are provided for researching the ALS inhibitor herbicide-resistant mechanism of the polypogon fugax, detecting the resistance of the polypogon fugax and determining the medication period and the dosage.

The invention relates to the technical field of microorganism application, in particular to bacillus velezensis, antibacterial lipopeptide and application of the bacillus velezensis and the antibacterial lipopeptide. The invention provides bacillus velezensis BGMW2 separated from a mangrove forest water area in Qinzhou city in Guangxi Zhuang Autonomous District, and the preservation number of the bacillus velezensis BGMW2 is GDMCC No: 64790. The strain BGMW2 has broad-spectrum antibacterial activity, and can significantly inhibit pathogenic bacteria such as escherichia coli, salmonella typhimurium, shigella, bacillus licheniformis and staphylococcus aureus. According to the invention, an antibacterial lipopeptide is separated from a metabolite of the strain BGMW2, and the antibacterial lipopeptide is separated, purified and identified as C12surfactin. The antibacterial effect of the C12surfactin is superior to that of a common bacteriostatic agent Nisin, and can be comparable with that of gentamicin sulfate. The C12surfactin also has the characteristic of stable antibacterial property at the temperature of between 40 and 80 DEG C and the pH value of between 2.0 and 12.0, and has hydrolase resistance and low hemolytic activity. The bacterial strain BGMW2 and a metabolite antibacterial lipopeptide thereof provided by the invention have important application prospects in the fields of food, pharmacy, disinfection, protection, health care products, cosmetics and the like.

The invention discloses an intestinal bacterial composition for improving a cognitive function and relieving neuroinflammation and an application of the intestinal bacterial composition, and belongs to the technical field of biomedicine and microbiology, the intestinal bacterial composition is composed of a Lachnospiraceae bacterium, a Bacteroides multiforme and a Roseburiatestinae bacterium, and the intestinal bacterial composition is prepared from the following raw materials of the following raw materials of the intestinal bacterial composition: the Lachnospiraceae bacterium, the Bacteroides multiforme and the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium and the like. Experimental research results show that a modular synergistic effect exists among the helicobacter hirsuticus, the bacteroides multiforme and the Roche enterobacter, and the pharmaceutical composition has a remarkable neuroprotective effect on a cognitive impairment mouse model and an Alzheimer disease mouse model, can improve the cognitive function and relieve neuroinflammation, and can be used for preparing a neuroprotective agent for treating the cognitive impairment mouse model and the Alzheimer disease mouse model. A new thought and method can be provided for treatment of cognitive impairment related diseases and neuroinflammation related diseases.

The invention provides an organic carrier in-root-rhizosphere growth-promoting microbial agent for ecological restoration of degraded grassland as well as a preparation method and application of the microbial agent, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth-promoting microbial agent disclosed by the invention is prepared from four kinds of growth-promoting bacteria (PGPE) in roots, four kinds of growth-promoting bacteria (PGPR) in roots and an organic carrier, the PGPE comprises terracotta warrior bacillus K4, bacillus 9-3-1, bacillus deserticola 11-5-4 and halomonas L31, the PGPR comprises serratia rubra 5, serratia rubra 23, cosinia cosinii 11 and enterobacter parabarium 24, and the organic carrier is a carbon-based organic fertilizer, a cattle and sheep manure organic fertilizer or biochar. When the organic carrier growth-promoting microbial agent is applied to the degraded grassland, the biomass and coverage of plant communities can be remarkably improved. The organic carrier growth-promoting microbial agent can be used for ecological restoration of degraded grassland.

The invention discloses an L-aspartic acid transaminase mutant and application thereof. The L-aspartic acid transaminase mutant is obtained by performing single mutation on the 104th amino acid of the amino acid sequence of L-aspartic acid transaminase. The L-aspartic acid transaminase mutant is used for synthesizing an L-phenylglycine derivative. The L-aspartic acid transaminase mutant is from escherichia coli, homologous sequence analysis and molecular docking simulation are carried out, site-specific mutagenesis verification is combined, and single-point mutation is carried out on the 104 amino acid to obtain the T104S mutant. The L-aspartic acid transaminase mutant disclosed by the invention can be used for efficiently catalyzing to generate an L-phenylglycine derivative, and compared with a wild type, the specific activity of the T104S mutant to o-chlorophenoxyacetic acid is 3.2 times that of the wild type.

The invention discloses a recombinant human IV-type collagen as well as a preparation method and application thereof, and belongs to the field of bioengineering and protein engineering. According to the invention, three recombinant human IV type collagen new molecules are designed and screened on the basis of a part of sequences in a human IV type collagen alpha1 chain, soluble expression of a target protein is realized by using an escherichia coli system, and the recombinant collagen with the purity of more than 98% can be obtained through nickel column purification and acid regulation treatment. The recombinant collagen provided by the invention has good cell migration promotion activity and cell adhesion activity, and can be widely applied to the fields of tissue engineering, medical equipment, cosmetics, health care products and the like.

The invention relates to the technical field of microorganisms, in particular to lactobacillus plantarum PC (Lactobacillus plantarum) and application of the lactobacillus plantarum PC. The lactobacillus plantarum PC strain provided by the invention has the characteristics of excellent acid resistance, bile salt resistance and the like, and is a multifunctional strain. The strain itself and metabolites thereof have multiple effects of relaxing bowel, reducing blood sugar, resisting oxidation and resisting bacteria, can shorten the defecation time of mice, improve the defecation number, promote the propulsion rate of small intestines, improve the water content of excrement, inhibit alpha-amylase and alpha-glucosidase, and can remove hydroxyl free radicals, DPPH free radicals and O2 <->. Multiple pathogenic bacteria and putrefying bacteria such as escherichia coli and staphylococcus aureus can be inhibited. The lactobacillus plantarum PC and the metabolite thereof provided by the invention can be applied to preparation of food, health care products, medicines and feeds with the characteristics of relaxing bowel, reducing blood sugar, resisting oxidation and resisting bacteria, and have wide application prospects and application values.

The invention discloses intestinal bacteria and serum metabolites which can be used as biomarkers of type 2 diabetes mellitus (prediabetes mellitus (PDM), new diagnosis diabetes mellitus (NDDM) and diabetes mellitus (P2DM) after medication) in different disease stages, and application of the intestinal bacteria and the serum metabolites. Aiming at type 2 diabetes mellitus, predictive markers including staphylococcus aureus, escherichia coli, klebsiella pneumoniae, branched chain amino acids (leucine, valine and isoleucine) and the like, especially staphylococcus aureus of which the genome contains acetolactate synthase (ALS), are efficiently screened out. The type 2 diabetes mellitus marker disclosed by the invention can be used for early warning the illness risk of type 2 diabetes mellitus and guiding the adjustment of an intestinal microbial environment; the method can be used for indicating the correlation between the intestinal microbial environment and the type 2 diabetes mellitus and is used for assisting diagnosis; and drug action target research, accurate medication, pathogenesis research and the like are realized.

The invention relates to the technical field of rapid detection methods, in particular to a one-step detection method for urinary tract infection escherichia coli with a non-disease diagnosis or non-treatment purpose. According to recombinase polymerase amplification provided by the invention, target DNA is specifically amplified through primers, CRISPR / Cas12a specifically recognizes a target sequence through crRNA, and through double screening of RPA and CRISPR / Cas12a, the target sequence is specifically recognized through crRNA; the rapid, sensitive and specific detection on the Escherichia coli infected with the urinary tract can be realized. Meanwhile, an RPA primer, crRNA and reaction conditions are optimized, so that an RPA reaction system and a CRISPR / Cas12a reaction system can be premixed in a tube, and the reaction can be completed only by a one-step method. The method can specifically detect urinary tract infection escherichia coli and has no cross reaction with common pathogenic bacteria, optimized conditions can avoid the competitive effect of CRISPR / Cas12a and RPA, the reaction rate and sensitivity are guaranteed, and meanwhile, the risk of nucleic acid aerosol pollution is also avoided due to the fact that a cover does not need to be opened.

The invention belongs to the technical field of synthetic biology, and particularly relates to a method for artificially synthesizing vanillin by taking glucose as a substrate. According to the method, escherichia coli transformed bacteria transformed with a ferulic acid efficient synthesis module TTF, a ferulic acid synthesis vanillin function module AEL and a ferulic acid synthesis vanillin function module ODR are utilized, and the vanillin is prepared through fermentation with glucose as a substrate. The engineering bacterium constructed by the invention can efficiently synthesize and prepare natural vanillin, has a relatively prominent yield effect, and shows a relatively good application prospect. The microbial fermentation method is low in production cost, mild in preparation condition and low in preparation cost, and the prepared vanillin belongs to natural vanillin and can better meet the requirements of the fields of food, medicines, cosmetics and the like on natural vanillin, so that the method has better technical popularization and application significance.

The invention discloses a double-structural-domain recombinant human fibronectin, the sequence of which is shown as SEQ ID NO.3, and the double-structural-domain recombinant human fibronectin is formed by fusing a collagen binding structural domain and a cell binding structural domain of human fibronectin. The invention also provides a recombinant expression vector containing the protein coding gene and a host cell. The protein is efficiently expressed through eukaryotic cells and engineering bacteria escherichia coli, and a fermentation and purification process suitable for an industrial scale is established in a matched manner. Functional experiments prove that compared with commercially available fibronectin products with a single structural domain, the double-structural domain recombinant human chemical fibronectin disclosed by the invention can remarkably promote proliferation, migration, adhesion and differentiation activity of human skin fibroblasts, and has wide application potential in the fields of skin repair, skincare and biomedical materials.

The invention relates to the technical field of preparation of viscose fibers, in particular to a preparation method of viscose fibers based on wormwood essential oil / paraffin double-layer microcapsules, which comprises the following preparation steps: S1, preparing the wormwood essential oil / paraffin double-layer microcapsules; s2, preparing a microcapsule spinning solution; s3, spinning and forming. According to the invention, the wormwood essential oil is coated in the double-layer microcapsule, so that the natural antibacterial property of the wormwood essential oil is fully exerted. Main active components (such as eucalyptol and flavonoid compounds) of the wormwood essential oil are continuously released through a microcapsule slow release mechanism, and growth of common pathogenic bacteria such as escherichia coli and staphylococcus aureus is remarkably inhibited. Meanwhile, due to the double-layer structure (the polyurethane inner shell and the polyurea outer shell) of the microcapsule, volatilization and oxidation of the wormwood essential oil in the spinning process are effectively avoided, and the long-acting antibacterial effect is ensured.

The invention provides a probiotic composition for preventing and / or inhibiting oral pathogens as well as a derivative product, a preparation method and application of the probiotic composition, and belongs to the technical field of oral care. The probiotic composition comprises the following bacterial strains in parts by mass: 90 to 0.45 part of a lactobacillus rhamnosus bacterial strain A2114, 10 to 0.3 part of a plant lactobacillus bacterial strain A2124, 80 to 0.4 part of a pediococcus pentosaceus bacterial strain A2135, 250 to 0.25 part of a lactobacillus reuteri bacterial strain A21350, and 40 to 0.2 part of a plant lactobacillus bacterial strain A2109. The probiotic composition or the metabolite thereof can effectively inhibit oral pathogens such as streptococcus mutans, porphyromonas gingivalis, helicobacter pylori, escherichia coli and staphylococcus aureus. Therefore, the probiotic composition or the metabolite thereof shows a good application prospect in the aspects of preventing and relieving oral problems such as decayed teeth, periodontitis and ozostomia.

The invention discloses lactobacillus pentosus HS01 for preventing obesity and application of the lactobacillus pentosus HS01, and belongs to the technical field of microorganisms. The lactobacillus pentosus HS01 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is March 3, 2023, and the preservation number is CGMCC No.26736. The lactobacillus pentosus HS01 can be used for preparing the lactobacillus pentosus HS01. The 16SrDNA (Deoxyribose Nucleic Acid) sequence of the lactobacillus pentosus HS01 is as shown in SEQ ID NO. 1. The lactobacillus pentosus HS01 has good gastrointestinal tract tolerance and relatively strong oxidation resistance, can inhibit the growth of escherichia coli and staphylococcus aureus, and has the effects of regulating intestinal flora and relieving obesity and hyperlipidemia symptoms caused by high fat diet. By adopting the lactobacillus pentosus HS01 and the application thereof, the lactobacillus pentosus HS01 can be widely used for developing related probiotic functional products with the functions of inhibiting bacteria, preventing obesity and reducing blood fat.

The present invention relates to an enzyme catalyst for preparing a dicarboxylic acid and a method for preparing a dicarboxylic acid using the same. The enzyme catalyst is prepared by a method comprising the following steps: (1) carrying out fermentation culture on enzyme-producing microorganisms in a culture medium to obtain a crude enzyme solution, and (2) immobilizing the crude enzyme solution on a molecular sieve to obtain the enzyme catalyst, wherein the enzyme producing microorganisms are selected from candida tropicalis, candida sake, pichia pastoris, saccharomyces cerevisiae, lactobacillus brevis, corynebacterium glutamicum, bacillus subtilis, bacillus cereus, escherichia coli and pseudomonas aeruginosa. The method comprises the following steps: in a PBS buffer solution, mixing triglyceride with the enzyme catalyst, and carrying out catalytic reaction under stirring at 20-50 DEG C; and then carrying out hydrolysis reaction at the temperature of 250-300 DEG C under the pressure of 30-100 bar. According to the method, the enzyme catalyst and the molecular sieve are immobilized, so that other oxidants or catalysts are not required to be added while the dicarboxylic acid with higher yield is obtained.

Provided are a recombinant human interleukin-11 mutant, and a conjugate of the mutant conjugated to a chemical molecule and a use thereof. The recombinant human interleukin-11 mutant achieves soluble expression in an Escherichia coli expression system by means of an SUMO sequence tandem fusion method. The conjugate is obtained by the site-specific conjugation of the mutant to the chemical molecule at a mutant amino acid residue in position W147 by means of a covalent bond. The chemical molecule is at least one of polyethylene glycol, an alkanoic acid aliphatic chain, and a hydrophilic high-molecular polymer containing an alkanoic acid aliphatic chain. The conjugate retains the binding capacity to human interleukin-11 receptor A while demonstrating significantly reduced binding capacity to human IL-6 signal transducer receptor. The conjugate shows the ability to block the signaling between human IL-11 and IL-11 RA and between human IL-11 and GP130 either in vivo or in vitro and can be used for the treatment of associated diseases with tissue fibrosis as a clinical manifestation caused by elevated primary or secondary IL-11.

The invention relates to the technical field of cosmetics, and discloses a cosmetic composition, a preparation method and application of the cosmetic composition in anti-aging and anti-wrinkle skin. The preparation method comprises the following steps: expressing SKAP2 protein through prokaryote escherichia coli, extracting milk exosome, wrapping the SKAP2 protein into the milk exosome, and mixing the milk exosome containing the SKAP2 protein with trehalose dihydrate, water and CMC (Carboxymethyl Cellulose) to prepare a hydrogel preparation which can be used as a cosmetic composition applied to gel coating agents, masks, toner and dry powder patches. The anti-wrinkle effect is achieved by inhibiting the activity of matrix metalloproteinase 1 (MMP1).

The invention discloses a cancerogenic enterobacter with potassium dissolving and growth promoting effects and application thereof, the cancerogenic enterobacter is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30972. The strain can activate insoluble or insoluble potassium in soil to be absorbed and utilized by plants and promote crop growth. The enterobacter cancerogenic strain has stable flora number, can be effectively colonized in rhizosphere soil, has a rapidly available potassium content of 2.7 [mu] g / mL in a culture solution after 4 d of culture, has good potassium dissolving ability, can activate insoluble or insoluble potassium in soil, and improves potassium element conversion and absorption of crops. The enterobacter cancerogenic has a potassium dissolving effect, activates insoluble or indissolvable potassium, improves potassium element conversion and absorption of crops, and promotes plant growth and nutrient element absorption, so that the yield and quality of the crops are improved.

The invention relates to the technical field of genetic engineering, in particular to recombinant humanized collagen and a preparation method thereof. The recombinant humanized III-type collagen provided by the invention can be efficiently and stably expressed under three expression systems of yeast, escherichia coli and CHO cells, and has biological activity superior to that of commercially available collagen products. The recombinant humanized III-type collagen disclosed by the invention has structural characteristics and functional activity similar to those of natural collagen, so that the recombinant humanized III-type collagen has wide application prospects in the aspects of biomedical materials, tissue engineering products, cosmetics, foods, health care products or medicines, medical instruments, medical cosmetology and the like.

A method and application of Escherichia coli BL21star (DE3) BLBRTXST3 and its fermentation method for producing high-purity lactose-N-neotetraose, the invention relates to the technical field of human milk oligosaccharide production, Escherichia coli )BL21 star (DE3) BLBRTXST3 is deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with the deposit number CGMCC No. 32521 and the deposit date November 6, 2024. The address of the deposit institution is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Escherichia coli ( Escherichia coli )BL21 star (DE3) BLBRTXST3 is used in the fermentation production of lactose-N-neotetraose (LNnT). The present invention improves the yield of lactose-N-neotetraose, uses a chromatographic simulated moving bed to produce high-purity lactose-N-neotetraose with a purity greater than 94%, replaces the traditional solvent crystallization method, has simple separation and purification, is conducive to industrial production, and simultaneously produces linear lactose-N-neohexaose with a purity greater than 95%.

The invention relates to a broad-spectrum bacteriostatic lactic acid bacteria postbiotic preparation, a preparation method and application to laying hens, the lactic acid bacteria postbiotic preparation comprises lactic acid bacteria thalli and fermentation products of the lactic acid bacteria thalli, and lactic acid bacteria comprise lactobacillus plantarum LP45 and bifidobacterium animalis BAL531. The feed additive is prepared from lactobacillus plantarum LP45 and bifidobacterium animalis BAL531 through mixed fermentation and low-temperature vacuum drying, pathogenic bacteria such as clostridium perfringens, escherichia coli and salmonella can be inhibited, the abundance of beneficial bacteria in the intestinal tracts of chicken flocks is improved, the state of excrement is improved, chicken necrotic enteritis and diarrhea are prevented and treated, the intestinal health of laying hens is improved, and the laying hens are prevented and cured. The digestion and absorption efficiency of the feed is improved, and the laying rate is increased.

The invention relates to the technical field of microorganisms, in particular to application of butyric acid producing enterobacter as a probiotic for resisting eimeria tenella. In the invention, an antibiotic cocktail-induced eimeria tenella infection model is utilized to explore the effect of butyric acid-producing enterobacter DSM 26588 on eimeria tenella development. The enterobacter butyricum DSM 26588 inhibits the growth of gametes by inhibiting the expression of the eimeria tenella gamete EtGFAT gene; according to the present invention, the intestinal tract lesion caused by Eimeria tenella infection can be improved, the intestinal tract pathological injury can be alleviated so as to reduce the Eimeria tenella oocyst discharge amount, the ACI value is 162, and the good anti-coccidiosis effect can be provided.

The invention discloses pyridone endoperoxide with antibacterial and antiviral activity and application thereof, and belongs to the fields of organic compound synthesis and medical technology, food engineering and the like. The invention proves that the series of endoperoxides can effectively kill pathogenic bacteria, fungi and viruses, including propionibacterium acnes, escherichia coli, staphylococcus aureus, candida albicans, human papilloma viruses, herpes simplex viruses and the like. The internal peroxide designed by the invention has the capability of treating skin diseases, is expected to be developed into a first-line medicine for treating skin diseases caused by pathogen invasion, and mainly relates to bacterial skin diseases, fungal skin diseases and viral skin diseases caused by pathogen infection. Meanwhile, the series of endoperoxides can be used for treating oral infection, periodontitis, infection of shoulder and other joint operations, and bacterial infection such as pressure sores. In addition, the peroxides in the series can also be used in the fields of food preservation, disinfectant preparation and the like.

The invention belongs to the technical field of biology, and particularly discloses a high-temperature-resistant antibacterial protein Cystatin B sourced from marine fish as well as a preparation method and application of the high-temperature-resistant antibacterial protein Cystatin B. The high-temperature-resistant antibacterial protein Cystatin B is derived from pomfret, and the amino acid sequence of the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO. 1; the nucleotide sequence of the gene for coding the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO.2, and the sequences of primers adopted for cloning the gene are as shown in SEQ ID NO.3-4. The high-temperature-resistant antibacterial protein Cystatin B has a remarkable antibacterial effect on gram-negative bacteria such as escherichia coli, vibrio parahaemolyticus and edwardsiella tarda as well as gram-positive bacteria such as staphylococcus aureus; and after being treated at the temperature of 100 DEG C, the antibacterial agent still can keep relatively high antibacterial activity. The antibacterial protein is simple in preparation method, can replace traditional antibiotics, and can quickly and efficiently prevent and treat various common bacterial diseases in aquaculture.

The invention provides a pulsating anaerobic microorganism multi-phase fluidized bed hydrogen-electricity combined supply system and a use method, and belongs to the technical field of sewage treatment. The system comprises a constant-temperature liquid storage tank, an anode chamber, a hydrogen production reaction chamber and other key components, and smooth flowing of liquid and gas is achieved through pipeline connection. In order to improve the reaction efficiency, key components such as a distributor, a particle confinement network and a bubble generator are arranged in the system. According to the system, microorganisms such as escherichia coli and shewanella are inoculated to form an efficient biological membrane, and a nano Fe3O4 microbial membrane is added. The system combines pulsation and stable liquid flow, efficiency is improved, and energy consumption is reduced. And the electric energy of the microbial fuel cell is introduced into the hydrogen production reaction chamber and the super capacitor, so that the electric energy is utilized and recycled. And meanwhile, the monitoring computer and the water quality detector are used for monitoring the generated voltage and water quality in real time. Chemical energy in sewage can be fully utilized, the energy conversion efficiency is improved, application with higher requirements is met, and meanwhile the reaction energy consumption is low.

The invention relates to the technical field of wastewater treatment microorganisms, in particular to an efficient compound microbial agent for municipal sewage treatment and application of the efficient compound microbial agent. The compound microbial agent is prepared from the following strains in percentage by mass: 22.5 to 24.3 percent of anaerobic rope bacteria, 10.5 to 13.1 percent of outer bacillus, 6.8 to 7.3 percent of brevibacillus, 3.1 to 4.3 percent of bacillus, 2.3 to 2.5 percent of enterobacter, 1.8 to 2.4 percent of hyphomonas, 2.1 to 2.5 percent of spirillum rot, 1.8 to 2.1 percent of coxiella and 16.8 to 18.2 percent of other bacteria. Aiming at the problems that the existing municipal sewage pollutant removal efficiency is low, and microorganisms are difficult to adapt to water quality fluctuation, the compound microbial agent can adapt to water quality and water quantity fluctuation, and the treatment effect is stable; the COD (Chemical Oxygen Demand) degradation and nitrogen and phosphorus removal capabilities are excellent, and the metabolic capability is strong.

The invention discloses an expression system suitable for short-chain non-specific peroxidase and application of the expression system, and belongs to the technical field of enzyme engineering and genetic engineering. According to the expression system disclosed by the invention, a nucleotide sequence of short-chain non-specific peroxidase with SUMO and 8HIS tags is connected to a pET28a vector to obtain a recombinant vector, and escherichia coli BL21 (DE3) is transformed to obtain a genetically engineered bacterium; genetically engineered bacteria are subjected to two-stage temperature control culture in a ZYM5052 liquid culture medium, and meanwhile, 5ALA is added into the culture medium. According to the invention, an escherichia coli self-induction system is used, so that a shake-flask culture can grow at high density in a short time, the culture medium contains rich nutrient substances, and monitoring of cell density and adding of IPTG are not needed in the expression process. Meanwhile, a heme precursor 5ALA is added into an escherichia coli self-induction culture medium, and finally the high-activity short-chain non-specific peroxidase is obtained.