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53 results about "Enterobacter" patented technology

Enterobacter is a genus of common Gram-negative, facultatively anaerobic, rod-shaped, non-spore-forming bacteria of the family Enterobacteriaceae. It is the type genus of the order Enterobacterales. Several strains of these bacteria are pathogenic and cause opportunistic infections in immunocompromised (usually hospitalized) hosts and in those who are on mechanical ventilation. The urinary and respiratory tracts are the most common sites of infection. The genus Enterobacter is a member of the coliform group of bacteria. It does not belong to the fecal coliforms (or thermotolerant coliforms) group of bacteria, unlike Escherichia coli, because it is incapable of growth at 44.5 °C in the presence of bile salts. Some of them showed quorum sensing properties as reported before.

A method for predicting malt pyf based on metagenomic detection

The application discloses a malt PYF prediction method based on metagenome detection and belongs to the field of malt quality detection. Candida The relative abundance of Phytobacter The relative abundance of Curtobacterium The relative abundance of Kosakonia The relative abundance of Enterobacter The relative abundance of Fusarium The relative abundance of Aureobasidium The relative abundance of Acinetobacter The relative abundance of Klebsiella The relative abundance of The PYF potential is calculated, and the smaller the PYF potential is, the lower the degree of yeast pre-flocculation caused by the malt is. The application is applied to the yeast pre-flocculation degree prediction aspect, solves the problem that the existing malt PYF detection method cannot accurately reflect the yeast pre-flocculation condition in actual application, has the characteristics that the detection result is more accurate and can truly reflect the degree of yeast pre-flocculation caused by the malt.
Owner:TSINGTAO BREWERY CO LTD

Enterobacter agglomerans cluster movement and uses thereof

The application belongs to the technical field of new application of microorganisms, and particularly relates to an enterobacter and application of the enterobacter in improving inflammatory bowel disease. The strain is named LS-68, is preserved in the China General Microbiological Culture Collection Center, has a preservation date of October 28, 2024, and has a preservation number of CGMCC NO.32368. The strain has the ability of group movement, has strong adaptability to the intestinal environment, well tolerates the acidity of gastric juice, is sensitive to a plurality of commonly used antibiotics, and has a good improvement effect on colitis.
Owner:SHANGHAI LISHAN BIOPHARMACEUTICAL CO LTD

An ultra-sensitive detection composition of aquatic product pathogenic bacteria, application and detection method thereof

ActiveCN117431327BBiotechnologyStreptococcus pyogenes
The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.
Owner:HAINAN UNIV +1

A lactic acid bacteria combination suitable for green bean silage and a preparation method and application thereof

This invention discloses a lactic acid bacteria composition suitable for sesame silage, its preparation method, and its application, relating to the field of microbiology. The lactic acid bacteria composition is *Lactobacillus plantarum* (…). Lactiplantibacillus plantarum M141 and Enterobacter sieboldii ( Weissella paramesenteroides M143, compared to using *Lactobacillus plantarum* M141 or *Enterobacter sieboldii* M143 alone or commercial lactic acid bacteria preparations, the combination of the two lactic acid bacteria (addition amount 5.0 × 10⁻⁶) is more effective. 6 The cfu / g FM ratio yields the highest lactic acid, significantly reduces pH and ammonia nitrogen content, effectively inhibits the growth of harmful microorganisms (yeast), and improves the fermentation quality of sesame silage.
Owner:TARIM UNIV

A novel lactoferricin inducible soluble expression system and a method for constructing the same

PendingCN122104720ABacteriaTransferrinsEscherichia coliLactoferricin
The application discloses a novel lactoferrin inducible soluble expression system and a construction method thereof, and belongs to the technical field of biotechnology and genetic engineering, and the specific scheme is that human lactoferrin (LF) is expressed by probiotic Escherichia coli Nissle1917, the protein is purified after the expression position is determined, and anti-inflammatory activity is determined. The LF expression system constructed by the application provides a construction method of a prokaryotic expression stable human lactoferrin expression vector, the vector is introduced into probiotic Escherichia coli Nissle1917, soluble target recombinant human lactoferrin is obtained after induction fermentation expression, two-step purification and identification, and the application has the characteristics of high safety, low cost, easy operation and the like.
Owner:DALIAN POLYTECHNIC UNIVERSITY

Antibacterial healing-promoting photosensitizer, method of preparation and use

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.
Owner:THE FIRST AFFILIATED HOSPITAL OF JINZHOU MEDICAL UNIV

A biological coating with physical-mechanical and chemical antibacterial properties and a method for its preparation

The application discloses a kind of biological coating with physical mechanical antibacterial and chemical antibacterial performance and preparation method thereof, it is related to biomaterial technical field.The biological coating of the application is composed of TC4 nanofiber network and nano copper deposited in nanofiber network, and the preparation method is: using polishing-washing-sweeping process pretreatment TC4 titanium alloy;It is placed in alkali solution and heated in water bath to activate, and the alloy after activation is repeated polishing, washing, sweeping step, to obtain the substrate to be sputtered;The substrate is magnetron sputtered, and nano copper is deposited on its surface;After depositing nano copper, the substrate is annealed in inert atmosphere and cooled, and the biological coating with physical mechanical antibacterial and chemical antibacterial performance is prepared.The synergistic effect of the biological coating composed of nano copper and nanofiber network can effectively kill staphylococcus aureus, escherichia coli and other pathogenic microorganisms, and can be applied to oral implant, metal implant biomaterial as functional coating.
Owner:KUNMING UNIV OF SCI & TECH

Escherichia coli fermentation tail gas bottle gas-liquid separation device

This utility model relates to the field of gas-liquid separation technology and discloses a gas-liquid separation device for E. coli fermentation tail gas bottles. The device includes a separation tank, an inlet pipe connected to the left side of the outer wall of the separation tank, a fixing ring fixedly connected to the top inner side of the separation tank, two filter screens slidably connected to the top inner side of the separation tank, multiple springs fixedly connected between adjacent filter screens, and filter cotton fixedly connected between adjacent filter screens. A pull rod is slidably connected to the top of the separation tank, and a piston is fixedly connected to the bottom of the pull rod. A cleaning mechanism is provided on the left side of the separation tank. In this utility model, impurities are filtered by the filter screens, tiny liquid droplets in the tail gas are adsorbed by the filter cotton, and the piston squeezes the filter cotton to discharge the liquid, achieving efficient separation of liquid and gas, effectively filtering impurities and tiny liquid droplets, and improving the tail gas treatment efficiency.
Owner:SHANGHAI BAILIAN BIOMEDICAL TECH CO LTD

Triphenylamine amphiphilic fluorescent probe, preparation method and application thereof

This invention relates to the field of fluorescent probe technology, specifically to a triphenylamine amphiphilic fluorescent probe, its preparation method, and its applications. The triphenylamine amphiphilic fluorescent probe is synthesized in three steps: bromination, coupling, and condensation to construct a D-π-A molecular structure, producing far-red light emission at 650-660 nm and exhibiting aggregation-induced emission (AIE) properties. The pyridine group endows it with hydrophilicity and cationic characteristics, while the triphenylamine group endows it with lipophilicity and AIE properties. It can specifically bind to bacterial surface anions, enabling wash-free, high-contrast real-time imaging, and can also disrupt bacterial biofilms to exert antibacterial effects. The probe has a MIC as low as 1.5625 μM against bacteria such as Staphylococcus aureus and Escherichia coli, can be co-stained with acridine orange for verification, is compatible with conventional detection equipment, and achieves integrated bacterial diagnosis and treatment, solving the problems of fluorescence quenching and low emission peaks in traditional probes.
Owner:ANHUI XINHUA UNIV

A sensor with antioxidant and antifouling properties and a preparation method and application thereof

The application provides a sensor with antioxidant and antifouling properties and a preparation method and application thereof, and belongs to the technical field of marine chemistry. The sensor takes a glassy carbon electrode (GC) as a substrate, adopts polyaniline as a solid contact layer, and takes a calcium ion selective membrane (ISM) as a detection core. The innovation of the sensor is that a fucoidan-based functional polypeptide (PFp) is modified on the ISM to construct an antifouling layer. The PFp layer forms a triple synergistic defense mechanism of "anti-adhesion-sterilization-antioxidation". Experiments prove that the sensor can effectively inhibit the adhesion and colonization of bacteria such as escherichia coli and staphylococcus aureus and chlorella, and significantly slow down the oxidative decay of the sensing membrane. In addition, when the sensor is used for calcium ion detection in actual seawater, it exhibits excellent long-term stability and high precision, and provides an innovative strategy for continuous in-situ monitoring in complex media.
Owner:QINGDAO UNIV OF SCI & TECH

Preparation method of sheep-strengthening powder

The invention belongs to the technical field of feed additives, and particularly relates to a preparation method of sheep Zhuang powder, which comprises the following specific steps: preparing an astragalus extract, preparing berberine hydrochloride, and mixing for preparation. In the preparation process of the sheep strengthening powder, the feed additive is added, the feed additive effectively replaces the previous aureomycin, the antibacterial effect (MIC) of the feed additive on escherichia coli AZ1, escherichia coli 8-1 and salmonella sk77 is obviously better than that of the aureomycin, the dosage every time is smaller, animals are healthier, both pathogenic bacteria are killed, the immunity is improved, and the sheep strengthening powder is suitable for being used for preparing sheep strengthening powder. The feed is safe and compliant, has no residue and no withdrawal period, and has the effects of fattening and weight gaining and fierce eating and fierce growth.
Owner:BAODING KANGHENG AGRICULTURE & ANIMAL HUSBANDRY TECHNOLOGY CO LTD

Systems And Methods For Imaging Diverse Pathogenic Bacteria In Vivo With [18F]Fluoromannitol Positron Emission Tomography

Compositions for identifying a pathogenic bacterial infection include a mannitol compound with one or more substituents including radioisotopes. These radiopharmaceuticals, such as a positron-emitting mannitol analogue, [18F]fluoromannitol ([18F]FMtl), are rapidly taken up by gram-positive and gram-negative bacteria such as E. coli, S. aureus, A. baumannii, S. epidermis, P. mirabilis, S. enterica, K. pneumonia, E. faecium, E. cloacae, and M. marinum, but not non-active infection sites such as sterile inflammatory sites, cancer sites, etc. Administration of these radiopharmaceuticals to and subsequent imaging of patients, e.g., via positron emission tomography (PET), enables detection of deep-seated and difficult to manage bacterial infections, such as osteomyelitis and prosthetic joint infection, or in patients with sickle cell disease. [18F]FMtl injection detects and differentiates infection rapidly. The radiolabeled mannitol compounds can be produced via nucleophilic substitution reactions that are deployable on commercially available synthesizers, facilitating straightforward and wide accessibility, and counteracting unnecessary antibiotic use.
Owner:NEUMANN KIEL +3

Escherichia coli coated calcium carbonate composite powder, block and preparation method thereof

The application discloses an Escherichia coli coated calcium carbonate composite material powder, a block and a preparation method thereof. The preparation method comprises the following steps: mixing and stirring natural Escherichia coli expressing GFP and a buffer solution containing calcium ions to obtain a suspension; adding an aqueous solution containing carbonate ions into the suspension, and after the adding is completed, centrifuging, washing and drying to obtain the Escherichia coli coated calcium carbonate composite material powder. The application effectively combines the natural Escherichia coli expressing GFP and calcium ions to inhibit the crystal type conversion, and after the titration is completed, the centrifugation and washing are rapidly performed, so that the single vaterite powder with stable crystal type is successfully prepared, and then the composite material block is prepared through a cold sintering process. The operation method is simple, the cost is low, and the application is suitable for large-scale production, so that a new research idea is provided for preparing a large-size heterogeneous composite material which can realize the synergistic improvement of strength and toughness and has a macroscopic geometric shape.
Owner:CHINA HUBEI LONGZHONG LABORATORY +1

Micro-nano ozone water saturation and its application in the preservation of fruits and vegetables such as sweet potatoes and lotus seeds

This invention discloses micro-nano ozone bubble water and its application in the preservation of fruits and vegetables such as sweet potatoes and lotus seeds. The micro-nano ozone bubble water is prepared by thoroughly mixing gaseous ozone with a liquid medium and then aerating the mixture. The liquid medium is a 1%-3% (v / v) citric acid solution. The prepared micro-nano ozone bubble water exhibits good stability and excellent bactericidal properties in an acidic system, significantly inhibiting *Escherichia coli*, *Staphylococcus aureus*, and pathogens causing sweet potato stem rot and root rot. This technology can effectively extend the storage period of sweet potatoes, tangerines, and lotus seeds, reduce weight loss and decay, and maintain the firmness and nutritional content of fruits and vegetables through sterilization, metabolic regulation, and optimization of the microbial community structure, providing theoretical and technical support for the green preservation of agricultural products.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

Antibacterial and self-repairing concrete anticorrosive coating and preparation method thereof

The application discloses a kind of concrete anticorrosive coating with antibacterial and self-repairing and preparation method thereof, the anticorrosive coating includes the following weight parts of raw materials: 20-50 parts alicyclic epoxy resin, 100-150 parts vinyl resin, 30-50 parts multifunctional curing agent, 5-15 parts polyvinylamine resin, 10-25 parts silicone resin, 1-3 parts silane coupling agent, 0.5-2 parts initiator, 3-8 parts epoxy resin curing agent and 30-50 parts filler;The multifunctional curing agent includes the following weight parts of raw materials: 10-15 parts vinyl epoxy, 15-20 parts allyl thiocyanate and 5-15 parts allyl methyl disulfide.The concrete anticorrosive coating of the application contains multifunctional curing agent, which has thiocyanate group and disulfide group, the thiocyanate group can effectively inhibit the activity of escherichia coli and staphylococcus aureus, prevent escherichia coli and staphylococcus aureus from corroding the coating, and improve the service life of the anticorrosive coating;Disulfide group can undergo reversible rupture and recombination, with self-repairing ability.
Owner:CHINA RAILWAY SHANGHAI ENGINEERING GROUP MATERIALS IND &TRADE CO LTD +1

Microorganism, microbial agent, food, preparation method of food and method for inhibiting growth of pathogenic bacteria in vitro

The invention discloses a microorganism, a microbial agent, food and a preparation method of the food, and relates to the field of microorganisms. The microorganism is hansenula polymorpha, and the preservation number of the microorganism is CGMCC (China General Microbiological Culture Collection Center) No.30796. The hansenula uvarum new strain obtained through screening is high in growth speed and high in acid resistance and salt resistance, especially can normally grow in a 3 mM nitrite environment, can inhibit growth of escherichia coli and staphylococcus aureus and is high in nitrate metabolism capacity; the fermented food prepared from the new strain is low in salt content, nutritional, healthy and unique in flavor; a food fermentation process developed by utilizing the new strain is high in fermentation efficiency, short in production period and high in application value.
Owner:CHINA AGRI UNIV

A recombinant Bacillus subtilis strain producing high-activity β-galactosidase and its application

This invention relates to a recombinant Bacillus subtilis strain producing high-activity β-galactosidase and its application. The recombinant Bacillus subtilis strain uses Bacillus subtilis WG600 as the host strain, derived from... Enterobacter Using sp. β-galactosidase as the target gene, through the promoter (P) phrE Synergistic optimization of the NCS sequence (glnA-30bp, MLD42) and 5'-UTR (UTR13, UTR4, UTR7) enabled the fermentation enzyme activity of Ensp-β-galactosidase in Bacillus subtilis to reach over 500 U / mL, with a maximum of 584.2 U / mL. Compared with existing technologies, the enzyme activity has been greatly improved, representing the highest level of enzyme activity for β-galactosidase production in Bacillus subtilis, which can meet the demand for high-activity enzymes in industrial production.
Owner:JIANGNAN UNIV

Salmonella and anti-inflammatory repair function of lactobacillus salivarius and its application

The application discloses a lactobacillus salivarius ZY-01 with anti-salmonella and anti-inflammatory repair functions, the strain has been preserved in the China General Microbiological Culture Collection Center on March 9, 2026, and the preservation number is CGMCC No. 37873. The strain can inhibit salmonella, escherichia coli and staphylococcus aureus, can reduce the expression of IL-6, IFN-gamma and TNF-alpha pro-inflammatory factors by inhibiting the JAK-STAT signal pathway, and can promote the expression of IL-10 anti-inflammatory factors and EGF, EGFR and TGF-beta1 mucosal repair factors, so that intestinal anti-inflammation and mucosal repair are realized. The lactobacillus salivarius ZY-01, bacterial liquid, bacterial powder and fermentation product thereof can be used as core raw materials of animal feed additives, micro-ecological preparations and veterinary drugs, are used for preventing and controlling salmonella infection and improving intestinal health of animals in animal breeding, and have a wide application prospect.
Owner:YANGLING ZHONGYOU LIFE SCIENCES CO LTD

External preparation for inhibiting bacteria and inflammation and treating scald and its preparation device

PendingCN122140612AAntibacterial agentsAntimycoticsIrritationSulfanilamide
The application discloses a kind of external formula for bacteriostatic anti-inflammatory and treating scald and preparation device thereof, including yellow wax 31.2 parts, camphor oil 40.2 parts, pearl powder 14 parts, sanqi powder 14 parts, phenol 0.3 parts, chloramine acid 0.3 parts, the yellow wax is royal jelly wax, the camphor oil is refined sesame oil, the pearl powder is nanoscale pearl powder, the sanqi powder is superfine sanqi powder, the phenol and chloramine acid are all medicinal grade raw materials;The bacteriostatic anti-inflammatory effect of external ointment prepared by the present formula is remarkable, the bacteriostatic rate of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and candida albicans is all ≥99.5%, and the bacteriostatic ring diameter is better than existing sulfadiazine silver ointment, while having good quick anti-inflammatory effect, accelerates the healing time of Ⅱ degree scald wound, reduces scar formation rate, while greatly reducing local irritation and allergic reaction, far lower than existing western medicine, with good wound healing and safety advantage.
Owner:HEILONGJIANG YAOWANGTING PHARMACEUTICAL CO LTD

Genetically engineered escherichia coli for synthesizing l-histidine and preparation method thereof

PendingCN122278733AEscherichia coliHeterologous
This application relates to the fields of bioengineering and fermentation technology, and discloses a genetically engineered *Escherichia coli* strain that synthesizes L-histidine and its preparation method. The engineered strain uses *Escherichia coli* W3110 as the starting strain and is modified... hisG Gene release from feedback inhibition; knockout ushA and nrdD Genes reduce precursor consumption; inactivation slyA Genes reduce energy waste; heterogeneous integration zwf and gnd Genes and replacement pgi The gene promoter redirects carbon flow to the pentose phosphate pathway, enhancing the supply of key precursors PRPP and reducing agent NADPH; introducing courtE Genes promote product efflux. Combined with an optimized fed-batch fermentation process, this invention effectively solves the problems of insufficient precursor supply, uneven energy distribution, and product inhibition through a systems metabolic engineering strategy, increasing L-histidine yield and sugar-acid conversion rate, making it suitable for industrial production.
Owner:HENAN ZHONGYUAN YUZE BIOTECHNOLOGY CO LTD

Method for preparing multifunctional indigo and polyphenol extract from malan stem and leaf

Using the stems and leaves of *Indigofera tinctoria* as raw material, a combined high-pressure water extraction technology involving water spray washing and grading, along with steam explosion, was employed to effectively improve the release rate of active substances such as indole glycosides and polyphenols, as well as the conversion rate of indigo. Multifunctional indigo and polyphenol extracts were prepared through bio-fermentation and membrane fractionation purification. The indigo extract contained significantly more bioactive substances than traditional lime-based indigo powder and indigo acidified compounds, with 15 times more indigo, 25 times less ash, and 650 times less calcium. Compared to natural Indian indigo, it exhibited superior dyeing performance on cotton fabrics and showed better inhibitory effects against *Aspergillus niger*, *Staphylococcus aureus*, and *Escherichia coli*. The *Indigofera tinctoria* polyphenol extract, composed of phenolic acids, phenylethyl glycosides, flavonoids, and polysaccharides, demonstrated excellent inhibitory activity against *Staphylococcus aureus* (IC50 = 32 μg / mL) and a wound healing rate exceeding 98% in mice, exhibiting good broad-spectrum antibacterial and wound-healing properties.
Owner:INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY

Integrated treatment method for treating sewage by microorganisms

PendingCN122444372AEscherichia coliDecomposition
The application discloses an integrated treatment method for treating sewage by microorganisms, and comprises the following steps: S1, sewage collection: concentrated oil film viscous sewage containing high-concentration high-temperature insoluble garbage is introduced into a first sewage pool, the first sewage pool is in the shape of a circular conical bottom pool, the sewage is naturally cooled to 20-25 DEG C in the first sewage pool, and the insoluble garbage in the sewage is preliminarily settled through the conical bottom structure. The application can directly target and break the oil film structure through a biological enzyme deodorant and a water quality purification enzyme compound microbial preparation, simultaneously degrade COD, total nitrogen, total phosphorus and ammonia nitrogen, supplement water quality decomposition agents and degrade by means of a standardized aerobic sewage pump for a second time, and finally, the COD can be reduced to below 100 mg / L, the oil film breaking rate and the pollutant removal rate are far superior to those of a traditional single microbial process, meanwhile, the microbial preparation can inhibit the reproduction of harmful bacteria such as escherichia coli and candida albicans, and decompose foul-smelling gases such as hydrogen sulfide and methyl mercaptan, and additional deodorizing and sterilizing equipment is not needed.
Owner:HEYUAN SUNSHINE BIOTECHNOLOGY CO LTD

Ggeago-based dna molecular cloning method and application thereof

The application belongs to the technical field of biotechnology, and particularly discloses a DNA molecular cloning method based on GgeAgo and application thereof. The method comprises the following steps: a vector plasmid is cut by a cutting system; the cutting product of the vector plasmid is mixed with an exogenous fragment with a homologous region, and then a recombination-based cloning method is used for assembly, followed by transformation of Escherichia coli to generate a recombination plasmid. The cutting system comprises a guide nucleic acid molecule and a programmable endonuclease GgeAgo or / and a mutant thereof. The GgeAgo cuts the plasmid under the guidance of the guide nucleic acid molecule to linearize the plasmid, an exogenous fragment with a homologous region is added, and then Escherichia coli is transformed to realize efficient and accurate insertion of the exogenous fragment into the target plasmid. The molecular cloning method provided by the application has high efficiency, short time and simple operation, and effectively reduces the mutation caused by PCR. The molecular cloning method provided by the application provides a new idea for the Ago-based molecular cloning technology and the synthesis and assembly technology of DNA.
Owner:HUBEI UNIV

Broad-spectrum bacteriophage targeting mucoid escherichia coli, cronobacter and salmonella and applications thereof

The application discloses a broad-spectrum bacteriophage targeting mucoid Escherichia coli, Cronobacter and Salmonella and application thereof Escherichia coli phage vB EcoP 3BB7B, the bacteriophage can specifically lyse colanic acid-producing mucoid Escherichia coli, and breaks through the technical bottleneck that a mucous layer limits adsorption of most bacteriophages; further has cross-species lysis capacity, not only has good lysis activity on mucoid Escherichia coli, but also can effectively lyse important foodborne pathogenic bacteria such as Salmonella of various serotypes and Cronobacter sakazakii, and significantly widens the host spectrum range. The bacteriophage can realize efficient amplification under the condition of a lower infection multiple, has good environmental stability, has strong lysis activity, does not carry drug resistance genes and virulence-related genes, has high safety, and has a good application prospect in clinical and food safety prevention and control.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

A selective solid blood culture medium for isolation of f. pyogenes and application thereof

PendingCN122405468AEscherichia coliHemin
This invention discloses a selective solid blood culture medium for isolating Cryptococcus pyogenes and its application. The culture medium is a solid medium containing 15.0 g / L tryptone, 10.0 g / L yeast extract, 0.5 g / L L-cysteine, 5.0 g / L sodium chloride, and 15.0 g / L agar. After sterilization and cooling to 50°C, 5% (v / v) defibrinated sheep blood, 0.010 g / L vitamin K, 0.010 g / L heme chloride, and 0.010 g / L colistin B are added. This invention's culture medium selectively inhibits Gram-negative bacteria such as Escherichia coli by adding colistin B. Simultaneously, by optimizing the nitrogen source, adding key growth factors such as L-cysteine, vitamin K, and heme chloride, and using a simple anaerobic gas-generating bag culture method, it can significantly improve the isolation efficiency of Cryptococcus pyogenes from clinical samples, forming typical colonies within 24-48 hours, facilitating rapid pathogen isolation.
Owner:HUNAN AGRI UNIV

Function and application of a lactic acid bacteria ligilactobacillus salivarius SunLab-GHL02

The present application belongs to the field of microbial technology, and particularly relates to a lactic acid bacteria strain and application thereof. The strain is Ligilactobacillus salivarius SunLab-GHL02 (L. salivarius GHL02 for short), which is isolated from the lungs of high-performance Arbor Acres broilers, has been preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251726. The classification status and functional characteristics of the strain are determined through morphological, physiological and biochemical, 16S rRNA and whole genome identifications. The strain has good acid tolerance, bile salt tolerance and acid production capacity, and can significantly inhibit pathogenic bacteria such as Escherichia coli, pathogenic E. coli, Salmonella, Staphylococcus aureus and Pseudomonas aeruginosa. Animal experiments show that atomization administration of the strain can improve the growth performance and feed conversion rate of poultry, enhance the stress resistance and promote health. The strain and its application can be widely used in the development of feed additives, probiotic preparations and animal health products, and are suitable for improving the health and production performance of poultry under intensive breeding conditions, and have good application prospects.
Owner:NORTHWEST A & F UNIV +1

Preparation method of escherichia coli extracellular vesicles and application thereof in anti-lung cancer drugs

The application discloses a preparation method of E. coli extracellular vesicles and application of the E. coli extracellular vesicles in anti-lung cancer drugs. The preparation method of the E. coli extracellular vesicles comprises the following steps: knocking out a Braun lipoprotein gene of E. coli to obtain a recombinant bacterium; lysing the recombinant bacterium and performing low-speed centrifugation to obtain a supernatant and a lysis solution; performing high-speed centrifugation on the lysis solution to obtain a precipitate, which is the E. coli extracellular vesicles. The E. coli extracellular vesicles prepared by the method can be applied to the preparation of anti-tumor drugs. The Braun lipoprotein gene of E. coli is knocked out, a recombinant bacterium strain is constructed, and OMVs extracted from the bacterium strain can directly inhibit the proliferation of specific types of small cell lung cancer NCI-H69, but the inhibiting effect on the proliferation of non-small cell lung cancer and other small cell lung cancer strains is poor. The OMVs prepared by the application can provide a new path for the design and screening of anti-lung cancer drugs, and have a good drug-making prospect.
Owner:XUZHOU MEDICAL UNIVERSITY

A method for driving a battery waste residue in-situ to generate amorphous metal sulfide

A method for in-situ generation of amorphous metal sulfides from battery waste driven by sulfate-reducing bacteria includes the following steps: S1, pretreatment of the battery waste; S2, ultrasonic treatment of the battery waste in choline-serine ionic liquid; S3, addition of a mixed bacterial seed culture medium to the battery waste for anaerobic reaction to generate amorphous metal sulfides. The mixed bacteria consist of desulfurized Vibrio broth, rumen-desulfurized Enterobacter rumen-desulfurized Enterobacter rumen-desulfurized microbacteria broth. This method for in-situ generation of amorphous metal sulfides from battery waste driven by sulfate-reducing bacteria can reduce sulfate from 1.75% to 0.17% in just 3 to 5 days, with a removal rate of 85.71% to 90.29%, thus rapidly and efficiently converting sulfate in battery waste into metal sulfides.
Owner:ENERGY CONSERVATION & ENVIRONMENTAL PROTECTION IND RES INST OF GUANGDONG CENT ENVIRONMENTAL PROTECTION ASSOC

An antistatic and antibacterial polyester filament and its preparation method

PendingCN122279789APolyesterPolymer science
This invention relates to the field of functional polyester fiber technology, and in particular to an antistatic and antibacterial polyester filament made from PET chips as a matrix, compounded with nano-silver-PET antibacterial masterbatch, polyetheramide-PET antistatic masterbatch, dispersant, composite antioxidant, and lubricant. The process involves raw material drying, metered blending, melt extrusion, filtration and metering, spinning and cooling, bundling and oiling, winding and forming, and optional stretching and deformation. This invention achieves integrated antistatic and antibacterial functions through masterbatch blending and melt spinning. The prepared polyester filament exhibits an antibacterial rate of ≥99% against Staphylococcus aureus and Escherichia coli, an antibacterial rate of ≥95% after 50 washes, and a surface resistivity of ≤10. 8 It maintains excellent mechanical properties and spinning stability while achieving Ω·cm; the preparation process parameters are controllable and suitable for continuous industrial production, with no post-processing pollution.
Owner:浙江独山能源有限公司

A traditional Chinese medicine gynecological bacteriostatic lotion with anti-inflammatory and antipruritic effects

This invention discloses a traditional Chinese medicine gynecological antibacterial wash with anti-inflammatory and antipruritic effects, belonging to the technical field of gynecological antibacterial washes. The wash is composed of selectively antibacterial traditional Chinese medicine extracts, prebiotic components, and a pharmaceutically acceptable carrier, with a pH value of 3.8-4.5. The selectively antibacterial traditional Chinese medicine extracts include dandelion extract, honeysuckle extract, and Cnidium monnieri extract. By setting the pH value to 3.8-4.5 and using selectively antibacterial traditional Chinese medicine extracts containing dandelion extract, honeysuckle extract, and Cnidium monnieri extract, this invention achieves inhibition zones ≥12mm in diameter against Candida albicans, Staphylococcus aureus, and Escherichia coli, and inhibition zones ≤4mm in diameter against Lactobacillus curvatureii and Lactobacillus japonicus. This effectively inhibits common gynecological pathogens while avoiding excessive killing of beneficial vaginal bacteria, thereby maintaining the vaginal microecological balance.
Owner:XIAN LEKOU FUTAI BIOMEDICAL TECH CO LTD