Patents
Literature
Patsnap Eureka AI that helps you search prior art, draft patents, and assess FTO risks, powered by patent and scientific literature data.

299 results about "Enterobacter" patented technology

Enterobacter is a genus of common Gram-negative, facultatively anaerobic, rod-shaped, non-spore-forming bacteria of the family Enterobacteriaceae. It is the type genus of the order Enterobacterales. Several strains of these bacteria are pathogenic and cause opportunistic infections in immunocompromised (usually hospitalized) hosts and in those who are on mechanical ventilation. The urinary and respiratory tracts are the most common sites of infection. The genus Enterobacter is a member of the coliform group of bacteria. It does not belong to the fecal coliforms (or thermotolerant coliforms) group of bacteria, unlike Escherichia coli, because it is incapable of growth at 44.5 °C in the presence of bile salts. Some of them showed quorum sensing properties as reported before.

Intestinal bacterium composition for improving cognitive function and relieving neuroinflammation and application thereof

The invention discloses an intestinal bacterial composition for improving a cognitive function and relieving neuroinflammation and an application of the intestinal bacterial composition, and belongs to the technical field of biomedicine and microbiology, the intestinal bacterial composition is composed of a Lachnospiraceae bacterium, a Bacteroides multiforme and a Roseburiatestinae bacterium, and the intestinal bacterial composition is prepared from the following raw materials of the following raw materials of the intestinal bacterial composition: the Lachnospiraceae bacterium, the Bacteroides multiforme and the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium and the like. Experimental research results show that a modular synergistic effect exists among the helicobacter hirsuticus, the bacteroides multiforme and the Roche enterobacter, and the pharmaceutical composition has a remarkable neuroprotective effect on a cognitive impairment mouse model and an Alzheimer disease mouse model, can improve the cognitive function and relieve neuroinflammation, and can be used for preparing a neuroprotective agent for treating the cognitive impairment mouse model and the Alzheimer disease mouse model. A new thought and method can be provided for treatment of cognitive impairment related diseases and neuroinflammation related diseases.
Owner:ZHEJIANG UNIV OF TECH

Organic carrier in-root-rhizosphere growth-promoting microbial agent for ecological restoration of degraded grassland as well as preparation method and application of organic carrier in-root-rhizosphere growth-promoting microbial agent

The invention provides an organic carrier in-root-rhizosphere growth-promoting microbial agent for ecological restoration of degraded grassland as well as a preparation method and application of the microbial agent, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth-promoting microbial agent disclosed by the invention is prepared from four kinds of growth-promoting bacteria (PGPE) in roots, four kinds of growth-promoting bacteria (PGPR) in roots and an organic carrier, the PGPE comprises terracotta warrior bacillus K4, bacillus 9-3-1, bacillus deserticola 11-5-4 and halomonas L31, the PGPR comprises serratia rubra 5, serratia rubra 23, cosinia cosinii 11 and enterobacter parabarium 24, and the organic carrier is a carbon-based organic fertilizer, a cattle and sheep manure organic fertilizer or biochar. When the organic carrier growth-promoting microbial agent is applied to the degraded grassland, the biomass and coverage of plant communities can be remarkably improved. The organic carrier growth-promoting microbial agent can be used for ecological restoration of degraded grassland.
Owner:INNER MONGOLIA UNIVERSITY

Double-structural-domain recombinant human chemical fibronectin as well as preparation and application of double-structural-domain recombinant human chemical fibronectin

ActiveCN121343009ACosmetic preparationsBacteriaEucaryotic cellHuman skin
The invention discloses a double-structural-domain recombinant human fibronectin, the sequence of which is shown as SEQ ID NO.3, and the double-structural-domain recombinant human fibronectin is formed by fusing a collagen binding structural domain and a cell binding structural domain of human fibronectin. The invention also provides a recombinant expression vector containing the protein coding gene and a host cell. The protein is efficiently expressed through eukaryotic cells and engineering bacteria escherichia coli, and a fermentation and purification process suitable for an industrial scale is established in a matched manner. Functional experiments prove that compared with commercially available fibronectin products with a single structural domain, the double-structural domain recombinant human chemical fibronectin disclosed by the invention can remarkably promote proliferation, migration, adhesion and differentiation activity of human skin fibroblasts, and has wide application potential in the fields of skin repair, skincare and biomedical materials.
Owner:BAYI MEIHENG (BEIJING) TECH CO LTD

Probiotic composition for preventing and / or inhibiting oral pathogens as well as derivative product, preparation method and application thereof

The invention provides a probiotic composition for preventing and / or inhibiting oral pathogens as well as a derivative product, a preparation method and application of the probiotic composition, and belongs to the technical field of oral care. The probiotic composition comprises the following bacterial strains in parts by mass: 90 to 0.45 part of a lactobacillus rhamnosus bacterial strain A2114, 10 to 0.3 part of a plant lactobacillus bacterial strain A2124, 80 to 0.4 part of a pediococcus pentosaceus bacterial strain A2135, 250 to 0.25 part of a lactobacillus reuteri bacterial strain A21350, and 40 to 0.2 part of a plant lactobacillus bacterial strain A2109. The probiotic composition or the metabolite thereof can effectively inhibit oral pathogens such as streptococcus mutans, porphyromonas gingivalis, helicobacter pylori, escherichia coli and staphylococcus aureus. Therefore, the probiotic composition or the metabolite thereof shows a good application prospect in the aspects of preventing and relieving oral problems such as decayed teeth, periodontitis and ozostomia.
Owner:SICHUAN AISHENG LIFE TECHNOLOGY CO LTD +2

Compound microbial agent for municipal sewage treatment and application thereof

The invention relates to the technical field of wastewater treatment microorganisms, in particular to an efficient compound microbial agent for municipal sewage treatment and application of the efficient compound microbial agent. The compound microbial agent is prepared from the following strains in percentage by mass: 22.5 to 24.3 percent of anaerobic rope bacteria, 10.5 to 13.1 percent of outer bacillus, 6.8 to 7.3 percent of brevibacillus, 3.1 to 4.3 percent of bacillus, 2.3 to 2.5 percent of enterobacter, 1.8 to 2.4 percent of hyphomonas, 2.1 to 2.5 percent of spirillum rot, 1.8 to 2.1 percent of coxiella and 16.8 to 18.2 percent of other bacteria. Aiming at the problems that the existing municipal sewage pollutant removal efficiency is low, and microorganisms are difficult to adapt to water quality fluctuation, the compound microbial agent can adapt to water quality and water quantity fluctuation, and the treatment effect is stable; the COD (Chemical Oxygen Demand) degradation and nitrogen and phosphorus removal capabilities are excellent, and the metabolic capability is strong.
Owner:SUZHOU XINLONG ECOLOGICAL ENVIRONMENT CO LTD

Expression system suitable for short-chain non-specific peroxidase and application thereof

The invention discloses an expression system suitable for short-chain non-specific peroxidase and application of the expression system, and belongs to the technical field of enzyme engineering and genetic engineering. According to the expression system disclosed by the invention, a nucleotide sequence of short-chain non-specific peroxidase with SUMO and 8HIS tags is connected to a pET28a vector to obtain a recombinant vector, and escherichia coli BL21 (DE3) is transformed to obtain a genetically engineered bacterium; genetically engineered bacteria are subjected to two-stage temperature control culture in a ZYM5052 liquid culture medium, and meanwhile, 5ALA is added into the culture medium. According to the invention, an escherichia coli self-induction system is used, so that a shake-flask culture can grow at high density in a short time, the culture medium contains rich nutrient substances, and monitoring of cell density and adding of IPTG are not needed in the expression process. Meanwhile, a heme precursor 5ALA is added into an escherichia coli self-induction culture medium, and finally the high-activity short-chain non-specific peroxidase is obtained.
Owner:BEIJING UNIV OF CHEM TECH

Bifidobacterium longum subsp. longum, and use thereof in inhibiting bacteria, relieving colitis, and mitigating inflammatory bone loss

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.
Owner:DIPROBIO (SHANGHAI) CO LTD

Lactobacillus paracasei with effect of relieving inflammatory bowel disease and application of lactobacillus paracasei

The invention discloses a lactobacillus paracasei strain with an effect of relieving inflammatory bowel diseases and application thereof, and belongs to the technical field of microbial fermentation. The strain is Lactobacillus paracasei JGSLP08, and the preservation number of the Lactobacillus paracasei JGSLP08 is CGMCC (China General Microbiological Culture Collection Center) NO.34274. The invention further discloses a preparation method of the Lactobacillus paracasei JGSLP08. The strain has the characteristics of strong acid resistance, bile salt resistance and gastrointestinal fluid resistance, can effectively inhibit salmonella typhimurium, escherichia coli and staphylococcus aureus, and has an excellent antioxidant effect. A DSS-induced colitis mouse model shows that the lactobacillus paracasei can effectively improve the DIA score of a mouse, regulate inflammatory factors, reduce oxidative stress injury, relieve inflammatory cell infiltration and protect the integrity of mucosal epithelium, so that the inflammatory bowel disease is relieved and / or treated.
Owner:AGRI INST OF AGRI JIANGXI PROVINCE

Recombinant collagen triple helix folding and stabilizing method

The invention belongs to the technical direction of protein engineering in the technical field of biology, and particularly relates to a triple-helix folding and stabilizing method for recombinant collagen, which is a technology for efficiently assisting correct folding of human recombinant collagen expressed by escherichia coli and forming a stable triple-helix structure. The technology mainly solves the common key technical problems of low folding efficiency, unstable triple helix structure and the like of the recombinant collagen in an escherichia coli expression system, and obviously improves the yield of the functional recombinant collagen. The method has important application value in the fields of biomedical materials, tissue engineering, beauty and skin care and the like, and an efficient and economical technical path is provided for industrial production of recombinant collagen.
Owner:POLAR RES INST OF CHINA +1

Application of marine phycobacterium LZ-14T in production of indole-3-acetic acid

The invention belongs to the technical field of microorganisms, and particularly relates to application of marine phycobacterium LZ-14T in production of indole-3-acetic acid. According to the present invention, the interphycobacterium LZ-14T is named as Maricatenella alexandrii gen. Nov., sp. Nov. The present invention discovers that the interphycobacterium LZ-14T can achieve the effect of significantly inhibiting the synthesis of the PSP toxin by releasing the IAA signal molecule under the condition of promoting the growth of the host algae. The new discovery can provide a new theoretical basis for analyzing the generation mechanism of paralytic shellfish poison of alexandrium chainchain and exploring scientific prevention and control of red tide. The invention also finds that the IAA produced by the phycobacterium LZ-14T can inhibit the growth of escherichia coli, and the result lays a foundation for the preparation of new antibacterial drugs.
Owner:ZHEJIANG OCEAN UNIV +1

HIV (Human Immunodeficiency Virus) combined non-alcoholic fatty liver diagnosis and prediction marker and application thereof

The invention belongs to the technical field of medical diagnosis, and provides an HIV (human immunodeficiency virus) combined non-alcoholic fatty liver diagnosis and prediction marker and application. The marker comprises 39 biomarkers in total, wherein the 39 biomarkers comprise two intestinal microorganisms of enterobacter and klebsiella and 37 plasma metabolites of diacylglycerol, lysophosphatidic acid and the like; by integrating microbiome and metabonomics, the multi-dimensional marker is screened and verified, so that the problems of lack of high specificity and sensitivity, difficulty in early disease screening and incapability of effective risk assessment of an HIV (Human Immunodeficiency Virus) combined NAFLD (Non-Amplified Fragment Leukemia) early diagnosis method caused by unknown pathogenesis in the prior art are solved; the marker combination is applicable to preparation of diagnostic kits, construction of disease prediction models and development of targeted therapy drugs, and a theoretical basis is provided for early discovery, risk early warning and advanced intervention of the HIV combined with the non-alcoholic fatty liver disease.
Owner:ZHEJIANG UNIV

Method for synchronously and rapidly detecting escherichia coli and multi-drug-resistant genes thereof

The invention provides a method for synchronously and rapidly detecting Escherichia coli and multiple drug-resistant genes thereof, which comprises the following steps: culturing a sample at night to obtain a bacterial solution to be detected, adding a buffer solution and a metal bath, centrifuging, taking a supernatant, detecting by micro-fluidic, optimizing the extraction process of nucleic acid in the sample, designing an optimal RPA primer probe group, and detecting the multiple drug-resistant genes of the Escherichia coli. A reasonable RPA coupled microfluidic detection system is established, synchronous and rapid detection of the escherichia coli uidA and I-type integrase gene intI1 thereof and drug-resistant genes sul1 and aadA5 is realized, the specificity is high, the sensitivity is high, the reaction time is short, the operation is convenient, and a method support is provided for monitoring and prevention and control of food-borne drug-resistant bacteria.
Owner:CHINA JILIANG UNIV +1

Copper-based multifunctional close-fitting textile and preparation method thereof

The invention discloses a copper-based multifunctional close-fitting textile and a preparation method thereof. The copper-based multifunctional close-fitting textile comprises a copper conductive fiber layer which is blended and woven with any other fibers; the natural fiber layer is selected from any natural fiber and is blended with the copper conductive fiber layer, the product integrates antifungal, deodorizing and dustproof synergy and is selectively applied to various use fields, and the blended spinning of the copper conductive fiber layer and the natural fiber layer can make the antistatic performance be improved in a breakthrough manner; in a dry environment, due to static electricity generated by friction in natural fibers, copper ions can quickly disperse electrostatic charges and guide the electrostatic charges to the ground through an electron conduction mechanism, and static electricity accumulation is eliminated. Dust prevention is realized by electrostatic adsorption of particles. The copper ions can destroy bacterial cell membranes and inhibit reproduction of the bacterial cell membranes, and can treat common bacteria such as escherichia coli and staphylococcus aureus and fungi such as trichophyton rubrum and trichophyton asthenia. The photocatalytic material is used for photothermal catalytic decomposition of peculiar smell molecules.
Owner:ZHUJI LVWEI TEXTILE CO LTD

A method for predicting malt pyf based on metagenomic detection

The application discloses a malt PYF prediction method based on metagenome detection and belongs to the field of malt quality detection. Candida The relative abundance of Phytobacter The relative abundance of Curtobacterium The relative abundance of Kosakonia The relative abundance of Enterobacter The relative abundance of Fusarium The relative abundance of Aureobasidium The relative abundance of Acinetobacter The relative abundance of Klebsiella The relative abundance of The PYF potential is calculated, and the smaller the PYF potential is, the lower the degree of yeast pre-flocculation caused by the malt is. The application is applied to the yeast pre-flocculation degree prediction aspect, solves the problem that the existing malt PYF detection method cannot accurately reflect the yeast pre-flocculation condition in actual application, has the characteristics that the detection result is more accurate and can truly reflect the degree of yeast pre-flocculation caused by the malt.
Owner:TSINGTAO BREWERY CO LTD

Phage lysozyme as well as preparation method and application thereof

The invention belongs to the technical field of gene engineering, and discloses bacteriophage lysozyme as well as a preparation method and application thereof. The bacteriophage lysozyme provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 4 or a variant fragment with at least 80% homology with the fragment as shown in SEQ ID NO: 4. The antibacterial spectrum of the bacteriophage lysozyme covers a plurality of bacteria such as staphylococcus aureus, bacillus subtilis, escherichia coli, pseudomonas putida, vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus, and the bacteriophage lysozyme has the advantages of excellent antibacterial performance, high thermal stability and high acid and alkali tolerance, and can be used for preparing the antibacterial bacteriophage lysozyme. The antibacterial agent can be well applied to the fields of food, aquaculture, animal husbandry and the like.
Owner:THIRD INSTITUTE OF OCEANOGRAPHY STATE OCEANI C ADMINISTRATION

Zinc oxide nanoparticles / quaternary amine modified polystyrene nanospheres, and preparation method and application thereof

The application provides a kind of zinc oxide nanoparticles / quaternary amine modified polystyrene nanospheres and its preparation method and application.The application prepares carboxyl functionalized PS / PVP nanospheres with high monodispersity by environmentally friendly water dispersion polymerization method;ZnONPs are successfully loaded after complexing zinc ions with the surface PVP pyrrolidone ring of PS / PVP nanospheres and reducing with reducing agent, and the surface of nanospheres is modified with quaternary amine groups by amidation and further quaternary amination of the surface carboxyl groups of PS / PVP nanospheres.The preparation method is simple, and the reaction condition is easy to realize.The material obtained by the method of the application is polystyrene nanosphere surface loaded with zinc oxide nanoparticles and grafted with quaternary amine groups;it is applied to antibacterial, and zinc oxide nanoparticles and quaternary amine groups play a synergistic effect, so that the material shows high antibacterial activity and can effectively inhibit the proliferation of escherichia coli and staphylococcus aureus.
Owner:QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES)

A method for improving the efficiency of soy sauce tank fermentation

The application discloses a method for improving the fermentation efficiency of soy sauce pool type fermentation, which is characterized by the following steps: using the self-source microorganism in the fermentation tank to carry out fermentation outside the fermentation tank, combining with the fermentation liquid of Lactobacillus reuteri for synergistic fermentation, and then returning the fermentation liquid to the fermentation tank for pouring and utilization, so as to realize the promotion of the self-source microorganism in the fermentation tank to the soy sauce fermentation. The self-source microorganism is fermented and cultured by reducing the salinity outside the fermentation tank and adding a proper amount of starch raw material, and the fermentation liquid with high activity of protease can be prepared in a short time, which is returned to the fermentation tank for pouring and utilization, so as to improve the degree and efficiency of protein hydrolysis in the fermentation tank. The Lactobacillus reuteri uses the fermentation liquid of the soy sauce mash in the fermentation tank as the basic nutrient source and ferments together with a proper amount of white granulated sugar to produce the fermentation liquid with the function of inhibiting Escherichia coli, so as to resist the Escherichia coli pollution in the fermentation process of the self-source microorganism outside the fermentation tank, guarantee the safety of the returned pouring fermentation liquid, and further improve the production efficiency of the soy sauce pool type fermentation technology.
Owner:SOUTH CHINA UNIV OF TECH +1

Base-modified mRNA (messenger Ribonucleic Acid) and application thereof in cell-free protein synthesis system

The invention discloses base-modified mRNA (messenger Ribonucleic Acid) and application thereof in a cell-free protein synthesis system, and belongs to the technical field of nucleic acid modification. Natural nucleoside triphosphate is completely replaced by different types of basic group modified nucleoside triphosphate, mRNA with different chemical modifications is obtained through an in-vitro transcription method and purification, and the mRNA is put into an in-vitro cell-free protein synthesis system for reaction. A reaction element recombination system-PURE system based on prokaryote Escherichia coli purified protein and an eukaryote CFPS system taking rabbit reticulated red lysate as a material are mainly selected to detect the corresponding translation efficiency of mRNA modified by different bases in two cell-free protein synthesis systems respectively. Different regulatory activities may be shown in different cell-free protein synthesis systems when different modified nucleotides are doped into mRNA, base modified triphosphoric acid capable of improving the translation efficiency of an in-vitro protein synthesis system is screened out, and the stability of mRNA in the cell-free protein synthesis system and the corresponding protein expression level are improved.
Owner:TIANJIN UNIV OF SCI & TECH +1

Application of phencyqualinium bromide as bacteriostatic active component and preparation of phencyqualinium bromide

The invention relates to the technical field of pharmaceutical preparations, in particular to application of phencycloqualinium bromide as an antibacterial active component and a preparation of the phencycloqualinium bromide. It is found that benzene ring quinaldinium bromide can be used as an antibacterial active component and has an inhibiting effect on various common bacteria and fungi (including but not limited to staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, aspergillus niger, candida albicans and the like); triethanolamine or other bacteriostatic activity enhancers are mixed with the phencycloqualinium bromide, so that the bacteriostatic activity of the phencycloqualinium bromide can be remarkably improved; when a benzene ring quinaldinium bromide related preparation is prepared, other bacteriostatic agent components are not added, a good bacteriostatic effect can still be achieved, and the safety is higher.
Owner:YINGU PHARMA CO LTD

Phospholipase B from aspergillus fumigatus and preparation method thereof

The invention provides phospholipase B from aspergillus fumigatus and a preparation method thereof. The amino acid sequence of the enzyme is as shown in SEQ ID NO: 1, and a catalytic triad of the enzyme is composed of Ser-122, Asp-178 and His-211. The invention provides a coding gene (SEQ ID NO: 2) of the gene, an expression vector pGEX-6P-1 containing the gene, a host cell escherichia coli BL21 (DE3), and a corresponding recombinant expression and purification method. The enzyme can specifically hydrolyze sn-1 and sn-2 fatty acid ester bonds of a GPI anchor, so that GPI-APs on a membrane can be efficiently released. Compared with common PI-PLC, the PlbH has the remarkable advantages that the PlbH is more excellent in enzymatic property, more stable in effect, efficient in release capacity for various GPI-APs and the like, and a brand new tool enzyme which can replace PI-PLC and is complementary in action mechanism is provided for GPI-APs research.
Owner:ANQING NORMAL UNIV

Method, equipment and medium for preparing recoverable super-hydrophilic PVDF (polyvinylidene fluoride) composite membrane

The invention relates to the technical field of polymer membrane preparation, and particularly discloses a preparation method, equipment and medium of a recoverable super-hydrophilic PVDF composite membrane, and the preparation method comprises the following steps: S1, preparing a polymer base material; s2, carrying out dopamine functionalization treatment; s3, carrying out aldehyde group carbon dot-polycation grafting; s4, carrying out epoxy cross-linking curing; s5, shaping and post-processing; according to the composite membrane prepared by the preparation method disclosed by the invention, an oil-water separation test shows that the modified M-1. 5 membrane has excellent oil-water separation capacity, and the membrane still keeps stable separation flux after five times of separation circulation; an antibacterial test shows that an obvious bacterial inhibition ring is found around M-1. 5, it is indicated that the M-1. 5 composite membrane can prevent bacteria from approaching and inhibit growth of escherichia coli, a repairable test shows that PVDF-3, PAN-7 and PES-3 membranes have repairable performance, and the proposal of the scheme provides a new thought for rapid repair of damaged polymer membranes.
Owner:杨宾

Application of composite solid phosphorus-solubilizing bacterial agent in repairing cadmium-contaminated soil and improving soil quality

The application relates to the field of environment, resource utilization and agricultural biotechnology, and particularly relates to application of a composite solid phosphorus-solubilizing bacterial agent for repairing cadmium-polluted soil and improving soil quality, wherein the composite solid phosphorus-solubilizing bacterial agent comprises phosphorus-solubilizing bacteria, bone charcoal, culture medium and organic matrix; the phosphorus-solubilizing bacteria are Pantoea agglomerans CT-3 and Enterobacter hormaechei HS-6; the composite solid phosphorus-solubilizing bacterial agent is applied to Cd-polluted soil, and the biological availability of Cd in the soil is reduced by Pantoea agglomerans CT-3, Enterobacter hormaechei HS-6 and micron-nanometer multi-stage bone charcoal, and meanwhile, the soil quality is improved. The application can effectively promote the transformation of insoluble phosphate into soluble phosphate, so that the content of effective soil phosphorus is increased by 98.19%; on the other hand, the application can fix soil heavy metal Cd, so that the content of effective Cd in the soil is reduced by about 52.50%, has excellent Cd-fixing capacity, and can relieve the Cd stress of crops, and has good application value in repairing farmland Cd pollution, improving soil quality and environmental protection.
Owner:TIANJIN UNIVERSITY OF TECHNOLOGY +1

Enterobacter agglomerans cluster movement and uses thereof

The application belongs to the technical field of new application of microorganisms, and particularly relates to an enterobacter and application of the enterobacter in improving inflammatory bowel disease. The strain is named LS-68, is preserved in the China General Microbiological Culture Collection Center, has a preservation date of October 28, 2024, and has a preservation number of CGMCC NO.32368. The strain has the ability of group movement, has strong adaptability to the intestinal environment, well tolerates the acidity of gastric juice, is sensitive to a plurality of commonly used antibiotics, and has a good improvement effect on colitis.
Owner:SHANGHAI LISHAN BIOPHARMACEUTICAL CO LTD

An ultra-sensitive detection composition of aquatic product pathogenic bacteria, application and detection method thereof

ActiveCN117431327BBiotechnologyStreptococcus pyogenes
The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.
Owner:HAINAN UNIV +1

Electrochemical sensor for detecting escherichia coli in food and preparation method of electrochemical sensor

The invention belongs to the technical field of harmful microorganism detection, and particularly provides an electrochemical sensor for detecting escherichia coli (escherichia coli O157: H7) in food and a preparation method of the electrochemical sensor. According to the electrochemical sensor, the surface of a substrate electrode is sequentially modified with an electron transduction layer and a recognition molecular layer; wherein the electron transduction layer is formed by an infinite coordination polymer composite material of gold nanoparticles; the recognition molecular layer is a nucleic acid aptamer. The electrochemical sensor is high in sensitivity, good in selectivity and low in detection limit; compared with other detection methods, the method does not need incubation and can be directly used for in-situ and rapid detection of bacteria.
Owner:SHANDONG INST OF BUSINESS & TECH

Bacteriostatic composition, and bacterial agent, veterinary drug and feed containing same

The invention relates to the technical field of nano engineering and probiotics, in particular to a bacteriostatic composition and a fungicide, a veterinary drug and a feed containing the bacteriostatic composition. The invention provides a bacteriostatic composition prepared by simultaneously mixing nano iron sulfide and lactobacillus, which can protect the intestinal colonization ability of lactobacillus and other beneficial bacteria, so that the nano iron sulfide promotes bacterial ferroptosis of pathogenic bacteria in the intestinal tract. The nano iron sulfide and the lactobacillus synergistically inhibit the growth of pathogenic bacteria such as escherichia coli, salmonella typhimurium and staphylococcus aureus in vitro and in intestinal tracts, have no adverse effect on beneficial bacteria and host intestinal epithelial cells, and are beneficial to intestinal health. The invention provides a natural and efficient application mode, and has very high conversion value.
Owner:CHINA AGRI UNIV +1

A lactic acid bacteria combination suitable for green bean silage and a preparation method and application thereof

This invention discloses a lactic acid bacteria composition suitable for sesame silage, its preparation method, and its application, relating to the field of microbiology. The lactic acid bacteria composition is *Lactobacillus plantarum* (…). Lactiplantibacillus plantarum M141 and Enterobacter sieboldii ( Weissella paramesenteroides M143, compared to using *Lactobacillus plantarum* M141 or *Enterobacter sieboldii* M143 alone or commercial lactic acid bacteria preparations, the combination of the two lactic acid bacteria (addition amount 5.0 × 10⁻⁶) is more effective. 6 The cfu / g FM ratio yields the highest lactic acid, significantly reduces pH and ammonia nitrogen content, effectively inhibits the growth of harmful microorganisms (yeast), and improves the fermentation quality of sesame silage.
Owner:TARIM UNIV

Application of extracellular vesicles in antibacterial yoga clothes

The invention relates to application of extracellular vesicles in antibacterial yoga clothes, the application comprises that the extracellular vesicles are micro-fixed on the surface layer of yoga clothes fabric through a padding method or a spraying method, and the problems that chemical antibacterial agents (silver ions, graphene and the like) of existing yoga clothes are high in cost and large in skin irritation, and environment / health risks exist are solved. The extracellular vesicles are slightly fixed on the surface layer of the yoga clothes fabric through a padding method or a spraying method, and the extracellular vesicles can inhibit biological membranes formed by pathogenic bacteria such as staphylococcus aureus and escherichia coli on the surface layer of the yoga clothes fabric by interfering pathogenic bacteria quorum sensing. The fabric has the advantages that peculiar smell substances such as sulfides and fatty acid volatile substances generated by metabolism of bacteria in a sweat wet environment are reduced from the source, fabric peculiar smell control is achieved, compared with chemical antibacterial agents such as silver ions and graphene, biotoxicity risks and environmental accumulation hidden dangers are avoided, anaphylactic reactions caused by long-term contact between yoga clothes and skin can be remarkably reduced, and wearing safety is improved.
Owner:XIAMEN IYOGA SPORTSWEAR CO LTD +1

Application of Spirodela and microorganism in repairing cadmium contaminated water and reducing cadmium content in rice grain in cadmium contaminated paddy field

The application is the application of Spirodela and microorganisms in repairing cadmium-polluted water bodies and reducing cadmium content in cadmium-polluted paddy field rice grains. The specific steps are as follows: first, prepare Oscillospiraceae bacterial suspension, Enterobacter bacterial suspension and Pantoea bacterial suspension respectively; then mix the three kinds of bacterial suspensions in proportion to obtain a mixed bacterial suspension; then add the mixed bacterial suspension and Spirodela prepared in proportion to the cadmium-polluted water bodies to be repaired or the cadmium-polluted paddy fields to be repaired, and the Spirodela and the microorganisms form a "Spirodela-functional bacterial community" symbiotic system; the microorganisms further transport cadmium to the leaves by adsorption, transformation, leaf surface sedimentation or transpiration; finally, the Spirodela is concentrated for treatment, thereby repairing the cadmium-polluted water bodies or reducing the cadmium content in the cadmium-polluted paddy field rice grains. Through the application, the cadmium-polluted water bodies can be better repaired, and the cadmium content in the cadmium-polluted paddy field rice grains can be effectively reduced.
Owner:CHENGDU UNIV

A yeast biomass composition and uses thereof

A yeast biomass composition is formed by culturing a strain of yeast in a substrate such as whey permeate to product a yeast broth, dewatering the broth to produce yeast biomass, and hydrolysing the yeast biomass. The yeast biomass composition finds utility as a feed additive for broiler chickens to reduce the adherence of pathogenic bacteria like Salmonella and E.coli to the gut wall of the chickens, thereby reducing intestinal stress and improving life daily weight gain in the chickens.
Owner:NARINGTECH LTD