1105 results about "Enterobacter" patented technology

The invention discloses compound functional sugar with a function of adjusting intestinal flora. The compound functional sugar contains functional oligosaccharide, L-arabinose, dietary fiber and probiotics and is characterized in that bifidobacterium, lactobacillus and other beneficial bacteria can be proliferated; meanwhile, the proliferation of escherichia coli, enterococcus and other harmful bacteria is restrained to adjust the intestinal flora; and the small intestine peristalsis can be promoted, the defecating time can be shortened, the defecation quantity can be increased to play a role in defaecation.

The invention relates to the field of microbial detection and discloses a method for rapid detection of enterobacter sakazakii. The method comprises the steps of: adding immunized superparamagnetism nanometer magnetic beads into a sample, adding a stabilizer, and incubating for 45-60min at 30-40 DEG C, wherein the magnetic beads are specifically bonded and enriched on microbial food-borne pathogenic bacteria, namely the enterobacter sakazakii, in the sample; and measuring spinning-spinning relaxation time and calculating a value deltaT2 by taking a bacteria-free sample as a blank control. The method disclosed by the invention has high specificity and sensitivity to the detection of the enterobacter sakazakii, is rapid, and is particularly suitable to detection of low-concentration enterobacter sakazakii, especially the enterobacter sakazakii in dairy products; in addition, the method is convenient and simple to operate and has good reliability and low requirement on assorted equipment.

The invention discloses a traditional Chinese medicine inclusion compound with an antibacterial effect and a textile finishing method of the traditional Chinese medicine inclusion compound. The traditional Chinese medicine inclusion compound is prepared from traditional Chinese medicine extract liquid with the total ingredient percentage being 100 percent and beta-cyclodextrin through a saturated water solution method, wherein the traditional Chinese medicine extract liquid comprises the following ingredients in percentage by mass: 25 percent to 60 percent of honeysuckle, 15 percent to 50 percent of folium Artemisiae argyi, 20 percent to 35 percent of liquorice and 5 percent to 15 percent of scutellaria baicalensis. The traditional Chinese medicine inclusion compound and cross linking agents are added into chitosan acetic acid solution to be prepared into antibacterial finishing liquid. A simple rolling baking process is adopted for carrying out antibacterial finishing on the cellulose fiber, the process is simple, the finished textile has the broad-spectrum antibacterial performance on common germs such as staphylococcus aureus, escherichia coli and the like, and in addition, the water washing resistance performance is good.

L-glutamic acid is produced by culturing in a medium a microorganism belonging to enterobacteria and having L-glutamic acid productivity, into which a citrate synthase gene derived from a coryneform bacterium is introduced to produce and accumulate L-glutamic acid in the medium and collecting the L-glutamic acid from the medium.

The present invention provides ketolide derivatives, which can be used as antibacterial agents. In particular, compounds described herein can be used for treating or preventing conditions caused by or contributed to by Gram-positive, Gram-negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp. Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebaclerium, Bacillus or Enterobactericeae. Also provided are processes for preparing such ketolide derivatives, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention discloses an isoflavone compound in a tobacco rhizome and a preparation method and application thereof. The preparation method comprises the following steps of: crushing a tobacco rhizome sample, performing ultrasonic extraction for 3 to 5 times by using 95 percent ethanol, combining the extracts, filtering, performing reduced pressure concentration to obtain an extract, primarily separating the extract by using silica gel column chromatography, further separating the extract by adopting high performance liquid chromatography, and thus obtaining the required new compound. Antibacterial activity screening test results show that the compound has strong antibacterial effect on common proteus species, escherichia coli, staphylococcus, bacillus subtilis and the like. The compound used for cigarette tipping paper plays an obvious role in inhibiting microbes for polluting the cigarette tipping paper.

The invention discloses a polylactic acid antibacterial nanofiber membrane and a preparation method thereof, which belong to the technical field of functional spinning. An antibacterial agent is added into polylactic acid, and an electrostatic spinning technology is adopted to prepare the polylactic acid antibacterial nanofiber membrane with high antibacterial activity. The antibacterial agent in the fiber membrane is triclocarban (TCC). The nanofiber membrane comprises 95 or 94 mass percent of polylactic acid and 5 or 6 mass percent of antibacterial activity TCC. The obtained antibacterial fiber membrane can inhibit over 93 percent of staphylococcus aureus, escherichia coli and candida albicans. The antibacterial fiber membrane can be applied to the fields of daily use, spinning, industry and medicaments.

The invention discloses a fourteen-food-borne pathogenic bacterium multiplex PCR detection primer set and a kit. The detection primer set is composed of primer pairs for detecting salmonella, Shigella, Vibrio parahemolyticus, campylobacter jejuni, campylobacter coli, staphylococcus aureus, bacillus cereus, Listeria monocytogenes, yersinia enterocolitica, enterobacter sakazakii, Escherichia coli, vibrio cholerae, Escherichia coli O157, aeromonas hydrophila and internal positive control. A multiplex PCR detection method based on an ordinary PCR platform is built, multiplex PCR reactions are carried out on genomic DNA, extracted from a sample to be tested, of bacteria in the same reaction system through the primer pairs acquired through analysis and design, and whether the food-borne pathogenic bacteria are contained in the sample or not is judged through the electrophoretic analysis of reaction products.

The invention discloses a loop-mediated isothermal amplification detection primer group, a detection method and a detection kit for enterobacter sakazakii. Aiming at ompA genes of the enterobacter sakazakii, in the invention, a specific detection primer group, a detection kit containing the detection primer group, and a detection method utilizing the detection kit are designed and selected, so that detection is carried out on samples to be detected, especially milk powder, thus determining whether the specific gene segments of the enterobacter sakazakii exist, and further determining whether the samples to be detected contains the enterobacter sakazakii. According to the invention, the detection kit and the detection method have the advantages of high sensitivity, strong specificity, short detection time, without a PCR (polymerase chain reaction) instrument and an electrophoresis apparatus, are simple for operation process, are especially suitable for a basic detection mechanism and food enterprises for self check, and have important meaning for fast detecting milk powder and ensuring the safety of the milk powder.

The present invention provides derivatives of ethanologenic Escherichia coli K011 constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.

The antimicrobial activity of bovine bactericidal/permeability-increasing protein (bBPI)-derived synthetic peptides against mastitis-causing Gram-negative bacteria was evaluated. Three peptides were synthesized with sequences corresponding to amino acids 65-99 (bBPI_65-99), 142-169 (bBPI_142-169), or the combination of amino acids 90-99 and 148-161 (bBPI_{90-99,148-161}) of bBPI. The bBPI_{90-99,148-161} peptide demonstrated the widest spectrum of antimicrobial activity, with minimum inhibitory (MIC) and bactericidal (MBC) concentration values ranging from 16-64 μg/ml against Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp, and 64-128 μg/ml against Pseudomonas aeruginosa. None of the peptides exhibited any growth inhibitory effect on Serratia marcescens. The antimicrobial activity of bBPI_{90-99,148-161} was inhibited in milk, but preserved in serum. Finally, both bBPI_142-169 and bBPI_{90-99,148-161} were demonstrated to completely neutralize LPS. The peptide bBPI_{90-99,148-161} is a potent neutralizer of the highly pro-inflammatory molecule bacterial LPS and has antimicrobial activity against a variety of Gram-negative bacteria.

The invention discloses a kit used for rapid detection of enterobacter sakazakii in milk, and applications thereof, and belongs to the field of food safety technology. The kit comprises a loop-mediated isothermal amplification (LAMP) reaction system, Bst DNA polymerase, a probe and a colloidal gold test strip. The kit is capable of solving a problem that result interpretation of existing LAMP detection methods is not capable of realizing on-site detection; result interpretation method of the test strip of the kit is simple, no instrument is needed, and interpretation can be realized by the naked eye.

Carbon nanotubes (CNTs) are promising nanomaterials that have the potential to revolutionize water and waste treatment practices in the future. The direct use of unbounded CNTs, however, poses health risks to humans and ecosystems because they are difficult to separate from treated water. Here, we report the design and synthesis of carbon nanotube ponytails (CNPs) by integrating CNTs into micrometer-sized particles, which greatly improves the effectiveness of post-treatment separation using gravitational sedimentation, magnetic attraction, and membrane filtration. We further demonstrate that CNPs can effectively perform major treatment tasks, including adsorption, disinfection, and catalysis. Using model contaminants, such as methylene blue, Escherichia coli, and p-nitrophenol, we show that all the surfaces of individual CNTs in CNPs are accessible during water treatment. Hierarchical structures containing CNPs can be employed in a multitude of nano-material engineering applications, such as water and waste treatment.

The invention provides a plant bacteriostatic composition and application thereof in cosmetics. The plant bacteriostatic composition is obtained by compounding a licorice extract, a honeysuckle extract and a Mangnolia officinalis extract. The plant bacteriostatic composition has good bacteriostatic effect and particularly has a synergistic effect of inhibiting escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger. The plant bacteriostatic composition can be applied to skin-care and washing cosmetics, reduces the adding amount of original preservatives and enables a product to have both antibacterial and anti-inflammatory effects.

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam lysinate delivered as an aerosol or dry powder formulation.

The invention relates to a culture medium for simultaneous composite enrichment of five kinds of food-borne pathogenic bacteria, namely Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella, and a preparation method for the culture medium. Food-borne pathogenic bacteria are a significant reason to cause food positioning, so the rapid and accurate detection of the food-borne pathogenic bacteria has an important practical significance for preventing and controlling food safety incidents. The culture medium is characterized by comprising the following components: 10.0 g of peptone, 10.0 g of sodium chloride, 9.0 g of disodium hydrogen phosphate, 1.5 g of monopotassium phosphate, 0.1 g of cholate, 0.1 mg of potassium tellurite, 1.0 g of lithium chloride, 3.0 g of glucose, 2.0 g of mannitol, 2.5 g of sodium pyruvate, 1.0 g of aesculin and 1,000 mL of distilled water, wherein the pH value is 7.1 to 7.5. The culture medium can simultaneously enrich five kinds of target pathogenic bacteria, can be used for separation and identification of target bacteria, can also be used for the molecular detection of multiple pathogenic bacteria on the same detection platform, provides technical support for a method for rapidly detecting five kinds of pathogenic bacteria in food, and meets the requirement of simultaneous detection of five kinds of food-borne pathogenic bacteria.

The invention discloses a tea tree essential oil antimicrobial gel for treating a vaginal disease in gynecology. Every 1000 parts by weight of gel contains the following raw material components: 1 to 100 parts of tea tree essential oil, 0.5 to 20 parts of emulsifier, 1 to 50 parts of biological adhesive material and the balance of modified components and purified water. The tea tree essential oil is added into the high-molecular biological adhesive material to carry out solubilizer screening, so that the obtained tea tree essential oil gel has good biocompatibility, and is small in irritation and has no delayed type hypersensitivity in an actual application process. The clinical test proves that after the prepared tea tree essential oil gel is squeezed into the vagina of a female by virtue of a gel applicator, the tea tree essential oil contained in the prepared tea tree essential oil gel can be used for effectively inhibiting staphylococcus aureus, escherichia coli and candida albicans in the vagina and the cervix uteri, and rapidly relieving itching, removing peculiar smells and performing restoration and regeneration; and by virtue of a buffering ability of the tea tree essential oil gel, the weak acidity of normal physiology of the vagina can be maintained, and the moisture content of the vagina is supplemented at the same time so as to exert a lubricating function.

The disclosed invention relates to an isolated hydrogen gas producing microorganism, termed Enterobacter sp. SGT-T4™ and derivatives thereof. Compositions and methods comprising the disclosed microorganisms are also provided. The disclosed invention also relates to a method to increase the hydrogen production rate and yield of hydrogen gas producing microorganism in the presence of diatomaceous earth and other absorptive materials. Further, the disclosure relates to the production of high microalgal biomass and microalgal oils suitable for economical industrial scale bio-diesel production from processed bacterial fermentation wastes as feedstock using the green microalga Chlorella protothecoides.

Particular aspects provide novel compositions and methods useful for the growth, isolation and detection of microorganisms that have α-glucosidase activity (e.g., the bacterium E. sakazakii). Certain embodiments provide a novel growth and/or plating media, comprising a fluorogenic α-glucosidase substrate, which is both selective for and differential to E. sakazakii. In particular embodiments, the α-glucosidase substrate comprises 4-methylumbelliferyl-α-D-glucoside. Additional embodiments relate to a selection media. Further embodiments relate to a selective medium that is based on Tryptone Bile agar. Still further embodiments relate to OK media as defined herein. Other embodiments of the invention relate to methods for growing bacterial cultures on media that is selective for and differential to microorganisms that have α-glucosidase activity (e.g., the bacterium E. sakazakii).

The invention relates to a bacterial strain for the high yield of succinic acid and application thereof. Escherichia coli is modified in order to remove or decrease the activities of adhE, ackA, pta, focA, pflB, iclR, ldhA, poxB and pepc genes; and the gene knockout bacterial strain then receives ultraviolet mutagenesis, and is screened by culture medium with glucose as the only carbon source, so that an engineering bacterial strain which can produce the succinic acid on basal medium and with the glucose as the only carbon source by anaerobic fermentation can be obtained. The invention adopts the succinic acid-producing escherichia coli as the starting strain, and by means of a rational design, the inveniton obtains the engineering bacterial strain with the glucose as the only carbon source theoretically, which carries out anaerobic fermentation in the basal medium and only produces the succinic acid and no other byproducts. Then by means of irrational metabolic evolution integrating ultraviolet mutagenesis with growth screening, a mutant strain for producing the succinic acid is obtained. The bacterial strain disclosed by the invention nearly produces no other heteroacids in the fermentation of the succinic acid, so the production cost of the succinic acid can be remarkably reduced.

Disclosed herein are various polypeptides that can be included in immunogenic compositions specific for pathogenic E. coli strains. The polypeptides have cellular locations which render them accessible to the immune system. The genes encoding the polypeptides were initially identified as being present in uropathogenic strain 536 but absent from non-pathogenic strains.