95 results about "Glucosidase activity" patented technology

The invention discloses a composite double-protein hypoglycemic peptide as well as a preparation method and application thereof, and belongs to the technical field of animal and plant source double-protein active peptides. The method comprises the following steps: performing computer simulation enzyme digestion and virtual screening by utilizing bioinformatics to determine a protein raw material and protease, performing enzymolysis on animal and plant double proteins (soybean protein and casein) serving as raw materials by adopting neutral protease, and performing separation, purification and structural identification, thereby obtaining the protein. The protein peptide fragment with high alpha-glucosidase inhibitory activity is obtained by virtually screening molecular docking, and the amino acid sequences of the protein peptide fragment are shown as SEQ ID No: 6, SEQ ID No: 9 and SEQ ID No: 24. The compound double-protein peptide sequence provided by the invention has an inhibiting effect on the activity of alpha-glucosidase, can be used for preventing and treating diabetes mellitus, and can be used for long-term health care or treatment of people with impaired glucose tolerance or diabetics as a functional food ingredient.

The invention relates to a plant lactobacillus and application thereof in fermentation of a grape seed extract. The strain is named as FBL002, and is preserved in the Guangdong Microbial Culture Collection Center, the preservation address is the 5th floor of the Experimental Building, No.100 Court, Middle Xianlie Road, Xiaoxian District, Guangzhou City, Guangdong Province, and the preservation number is GDMCC No.66015. The tolerance of the plant lactobacillus provided by the invention to active ingredients in the grape seed extract is improved by 34%, and meanwhile, the activity of beta-glucosidase is remarkably improved to 204U / mL. In fermentation application, the strain shows excellent biotransformation performance, the content of total phenols and the content of total flavonoids in the fermented grape seed extract are increased by 45% and 98% respectively compared with an unfermented system, and meanwhile, the scavenging rate of antioxidant activity ABTS free radicals and the scavenging rate of DPPH are remarkably increased to 95%. The strain shows important application value in the fields of functional food development and natural cosmetic raw material production, and an innovative solution is provided for high-value utilization of plant resources.

The invention relates to lactobacillus mucilaginosus and application thereof in fermented soybean milk. The lactobacillus mucus is preserved in the Guangdong Microbial Culture Collection Center on March 10, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No. 65866. The viable count of the fermented lactobacillus mucus in soybean milk is increased by 56%, meanwhile, the activity of beta-glucosidase is remarkably improved to 198 U / mL, and the degradation rate of total purine in soybean milk is 65.32%. In fermentation application, the total polyphenol content and the total flavone content in fermented soybean milk after fermentation are respectively increased by 39.56% and 52.32% compared with those in an unfermented system, the daidzein content and the genistein content in the fermented soybean milk after fermentation are respectively increased by 256.3% and 356.3% compared with those in the unfermented system, and meanwhile, the antioxidant activity ABTS free radical scavenging rate and the antioxidant activity DPPH scavenging rate are obviously increased to 89.6% and 94.8% respectively. The strain shows important application value in the fields of functional food development and natural cosmetic raw material production, and an innovative solution is provided for high-value utilization of plant resources.

The invention discloses a thiochromanone derivative containing a thiosemicarbazone structure and application of the thiochromanone derivative, relates to the technical field of medicines, and particularly relates to the technical field that the compound has better oxidation resistance and alpha-glucosidase resistance activity, the IC50 value of the compound 1-(7-chloro-4, 4-dioxothiochromanone-3-N-propyl) thiosemicarbazone to ABTS clearance activity is 2.86 [mu] g / mL, the IC50 value of the compound 1-(7-chloro-4, 4-dioxothiochromanone-3-N-propyl) thiosemicarbazone to alpha-glucosidase inhibition activity is 9.51 [mu] g / mL, and the effect is the best and is superior to that of a control agent vitamin E and acarbose. The thiochromanone derivative containing the thiosemicarbazone structure has a general formula shown in the specification,

The invention discloses trichosanthes kirilowii maxim seed polypeptide with alpha-glucosidase inhibitory activity and a preparation method thereof. Four Trichosanthes kirilowii Maxim seed polypeptides with alpha-glucosidase inhibitory activity are obtained through Trichosanthes kirilowii Maxim seed protein preparation, enzymolysis, separation and purification and identification, and the sequences of the four Trichosanthes kirilowii Maxim seed polypeptides are respectively HRQTWD, HANRLP, FGGVGER and SYAPEDR. Molecular docking analysis results show that interaction between the trichosanthes kirilowii maxim seed hydrolytic peptide and amino acid residues of an alpha-glucosidase active pocket is mainly based on hydrogen bonds, electrostatic interaction and hydrophobic interaction, and residues D203, D327, D443, F450, D542 and F575 are key binding sites interacting with the four polypeptides. The trichosanthes kirilowii maxim seed polypeptide with alpha-glucosidase inhibitory activity prepared by the invention can be used as a food-borne hypoglycemic component to be used for developing health-care foods and medicines for reducing blood sugar.

In the present application, by knocking out the BG gene in Trichoderma reesei PX3 (a strain of cellulase transcription activator that can be activated continuously), the BG activity can be reduced under the condition of not reducing or even increasing the filter paper enzyme activity of the enzyme system, and a low BG enzyme activity and high filter paper enzyme activity strain is constructed. This strategy can avoid the negative impact on cellulase production after BG knockout, and the obtained strain can be used for efficient production of cellobiose or as a chassis strain for producing cellulase.

The present application relates to a kind of chalcone amide alpha-glucosidase inhibitors and its preparation and application, the inhibitor structural formula is: preparation method specifically is (1) after 2-hydroxyacetophenone, sodium hydroxide and methyl p-formylbenzoate are reacted, intermediate in formula (a) is obtained after post-processing;(2) intermediate in formula (a) is dissolved in organic solvent and potassium hydroxide is reacted, after reaction, intermediate in formula (b) is obtained after post-processing;(3) intermediate in formula (b) and substituted aniline are dissolved in organic solvent, after reaction, the inhibitor shown in formula (c) is obtained after post-processing.Compared with prior art, the compound of the present application is novel, and experiments show that it has good alpha-glucosidase inhibitory activity, and can be used for preparing alpha-glucosidase activity inhibiting drug.

This invention belongs to the field of carbon nanomaterials technology, specifically disclosing a method for preparing and applying carbon dots from *Pu Di Lan* (a type of medicinal herb) residue. The specific steps are as follows: dried *Pu Di Lan* residue is pulverized using a cell wall breaker to obtain *Pu Di Lan* residue powder; the powder is then reacted using a hydrothermal method; the reaction solution is collected; the reaction solution is centrifuged, and the supernatant is collected; the supernatant is filtered, and the filtrate is collected; the filtrate is dialyzed, and the retaining liquid is collected; the retaining liquid is freeze-dried to obtain the *Pu Di Lan* residue carbon dots. The obtained *Pu Di Lan* residue carbon dots exhibit blue fluorescence under a 365 nm ultraviolet lamp, and their surface is rich in functional groups such as hydroxyl and carboxyl groups, showing good hydrophilicity. The *Pu Di Lan* residue carbon dots promote β-D-glucosidase activity and can be used in food processing; simultaneously, the fluorescence of the *Pu Di Lan* residue carbon dots is highly sensitive to β-D-glucosidase, and β-D-glucosidase activity can be detected by fitting a linear regression equation with a standard solution.

This invention relates to the field of soil treatment technology, specifically a method and its application for reducing compound fungicides in soil-plant systems by combining nano-silica and nitrification inhibitors. Nitrification inhibitors are used as a nitrogen management measure, and the application of nanomaterials influences soil abiotic properties and bacterial communities to drive the degradation of paclobutrazol and carbendazim. The application of nitrification inhibitors (DCD) produces multiple effects: significantly reducing carbendazim residues in soil-plant systems, paclobutrazol residues in soil, and NO3 in plants. ‑ -N and promote the content of soluble sugars and proline in plants. DCD reduces the activity of the soil electron transport system and β-glucosidase activity, while the addition of nano-SiO2 can mitigate this negative effect. Nano-SiO2 selectively enhances the performance of DCD in reducing compound fungicide residues in pak choi and reducing paclobutrazol residues, but hinders the degradation of carbendazim in pak choi.

The invention belongs to the field of food science and engineering, and discloses a preparation method of an active medicinal and edible composite nutrient AMCN with an anti-aging effect, and the preparation method comprises the following steps: putting a ganoderma lucidum monoclonal strain into a liquid culture medium for controllable culture to obtain supernate with beta-glucosidase activity of over 100mU / mL; and adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder, carrying out enzymatic biotransformation, carrying out lactobacillus plantarum fermentation, adding a freeze-drying protective additive, carrying out freeze-drying, grinding to obtain the active medicinal and edible composite nutrient AMCN, and storing the finished product in a dry and dark place. The active medicinal and edible composite nutrient can be used for preparing anti-aging functional foods or nutritional health-care products. A D-galactose induced zebrafish aging model verifies that the active medicinal and edible composite nutrient prepared by the application plays an anti-aging role by relieving oxidative stress, inhibiting cell aging and maintaining telomere stability, can reduce beta-galactosidase activity and intracellular ROS (reactive oxygen species) level, improves telomerase protein concentration, and has a good anti-aging effect. And the telomere length can be effectively recovered by medium and high concentrations.

The invention discloses a pyrimidine derivative containing a thiosemicarbazide structure and application of the pyrimidine derivative, and relates to the technical field of medicines, the compound has good oxidation resistance and alpha-glucosidase resistance activity, and the compound is 2-(4, 4 '-difluorophenyl)-2-(4, 4'-difluorophenyl)-2-(4, 4 '-difluorophenyl)-2-(4, 4' The IC50 values of the compound 2-(4, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1-methylsulfamide on DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity and ABTS (2, 2, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1-methylsulfamide are 3.96 mu g / mL and 2.01 mu g / mL respectively, the IC50 value of the compound 2-(4, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1-methylsulfamide on alpha-glucosidase inhibitory activity is 38.62 mu g / mL, the effect is best, and the compound 2-(4, 6-dimethyl pyrimidine-2-yl)-N-methylhydrazine-1 The pyrimidine derivative containing the thiosemicarbazide structure has a general formula shown in the specification, and is superior to control medicaments such as vitamin C, vitamin E and acarbose.

The invention discloses a low-GI biscuit with a blood sugar regulating function and a preparation method of the low-GI biscuit, and belongs to the technical field of food processing. The low-GI biscuits comprise water and oil wrappers and bakery shortening dough, and the water and oil wrappers comprise buckwheat flour, low-gluten flour, edible oil, yeast and baking soda; the bakery shortening dough comprises high amylose corn starch, corn starch, edible oil, ground cinnamon and salt. Through the synergistic effect of all the components, the activity of alpha-amylase and alpha-glucosidase is inhibited, carbohydrate digestion is delayed, and the GI value is reduced. The low-GI biscuit prepared by the preparation method disclosed by the invention has a crisp taste and an antibacterial effect, and a safe and effective food choice is provided for diabetics and crowds with blood glucose control.

This invention discloses a low glycemic index (GI) Hericium erinaceus (monkey head mushroom) steamed bun, belonging to the field of food processing and functional food applications. By weight, it is prepared from the following raw materials: 45-55 parts all-purpose flour, 28-35 parts Hericium erinaceus extract powder, 0.3-0.35 parts yeast, 15-18 parts water, 0.3-0.6 parts oil, and 0.7-0.9 parts sugar. This invention partially replaces the flour with Hericium erinaceus extract powder, fully utilizing the multiple mechanisms of action of Hericium erinaceus polysaccharides in forming a viscous gel in the digestive tract to slow down the contact between amylase and substrate, inhibiting α-amylase and α-glucosidase activity, and improving intestinal microecology and enhancing insulin sensitivity through dietary fiber. This allows the product to meet the low-GI food standard (GI<55), effectively filling the gap in low-GI steamed buns with Hericium erinaceus functional components as the core active ingredient. It has significant application value in the fields of diabetes management, weight control, and healthy staple foods.

The application discloses a kind of complex fermentation inoculant for improving equol production, fermentation liquor and application thereof, belong to microbial fermentation technical field.The complex fermentation inoculant of the application, streptococcus thermophilus BLCC2-0025 and lactococcus lactis MP1001 have high β-glucosidase activity, and low activity glycoside form is converted into high activity daidzein;Salivary lactobacillus LS1101 and lactobacillus gregius 0010 have the activity of further converting daidzein into equol;Four strains of bacteria are used in combination, which can synergistically improve the content of equol.The fermentation liquor prepared by fermenting pueraria extract with the complex fermentation inoculant of the application can target regulate intestine-ovary axis, significantly improve the reproductive function of POF mice, relieve ovarian oxidative stress and accurately regulate intestinal flora balance, providing a safe and effective preparation and application scheme for non-hormone intervention of premature ovarian failure.

The application provides a compound shown in formula I or a pharmaceutically acceptable salt thereof: wherein R4 is selected from a hydroxyl group, an O-beta-D Glc group, and an O-beta-D Glc-(2-1)-alpha-L-Rha group; and R8 is selected from a hydroxyl group, an O-beta-D Glc group, and an O-beta-D Glc-(2-1)-alpha-L-Rha group; and the application provides the use of the compound in the preparation of a blood sugar reducing drug and in the inhibition of alpha-glucosidase activity, and has the beneficial effect that the application provides the use of zeylasteroside D and zeylasteroside J in the preparation of a blood sugar reducing drug and in the inhibition of alpha-glucosidase activity, and discloses the compound structure of zeylasteroside L and the use of the zeylasteroside L in the preparation of a blood sugar reducing drug and in the inhibition of alpha-glucosidase activity.

The invention discloses preparation and application of a corn polypeptide with functions of inhibiting amyloglucosidase activity and promoting GLP-1 secretion, and belongs to the technical field of functional food. And the corn polypeptide is PYPR, PPAPR and PLRP (Poly Lactic Polymerase Chain Reaction). The corn polypeptide is obtained by enzymolysis of corn protein through alkaline protease, and is novel in structure and easy to prepare. The corn polypeptide can inhibit the activity of amyloglucosidase in vitro, can significantly promote GLP-1 secretion, and can up-regulate the expression of gcg and gc3 genes related to GLP-1 synthesis and a polypeptide recognition receptor GPR93 by regulating Ca < 2 + > influx and an ATP signal channel, thereby improving the steady state of blood glucose. The invention provides reference for developing functional food or health care products with the functions of reducing blood sugar and promoting secretion of insulin.

The present invention belongs to the field of genetic engineering technology, and in particular to a beta-glucosidase mutant, a nucleic acid molecule and an application thereof. The present invention provides a beta-glucosidase mutant, the amino acid sequence of the beta-glucosidase mutant being as shown in SEQ ID NO.1. The beta-glucosidase mutant of the present invention has mutation sites V268A, E290V and I450T compared with the wild-type beta-glucosidase, and the results of the examples show that the enzyme activity of the beta-glucosidase mutant of the present invention is 544U / L, which is 267.57% higher than the beta-glucosidase activity of 148U / L of the wild-type strain.

The present application relates to the field of biotechnology, and more particularly to a cassava peptide for inhibiting alpha-glucosidase activity and a preparation method thereof. The present application recovers cassava starch wastewater protein by acid precipitation method, and obtains high-activity alpha-glucosidase inhibitory peptides by heat and ultrasonic pretreatment of cassava protein to assist enzymatic hydrolysis. The peptide sequence is analyzed, the alpha-glucosidase inhibition type is determined, four cassava peptides with alpha-glucosidase activity are obtained, and their high alpha-glucosidase inhibitory activity in vivo and in vitro is verified. A new alpha-glucosidase inhibitory peptide is given, the blank in the prior art is overcome, and a new idea for high-value utilization of cassava processing wastewater is provided.

The invention belongs to the technical field of strigose hydrangea juvenile leaf active substance extraction, and provides a strigose hydrangea juvenile leaf active substance extraction method based on efficient cellulose decomposition bacteria, which comprises the following steps: collecting a soil sample, carrying out microorganism enrichment culture by taking strigose hydrangea juvenile leaf as a carbon source, separating and purifying a single strain, screening cellulose degradation dominant bacteria by adopting a Congo red staining method, and carrying out high-efficiency cellulose decomposition on the dominant bacteria. Then secondary screening is carried out through CMC enzyme activity and beta-glucosidase activity; with a water extraction method as a contrast, screening strains with the best effect of improving the active substances of the strigose hydrangea juvenile leaves; the influence of the inoculum size, the fermentation time and the material-liquid ratio on the extraction of the active substances is investigated through a single-factor experiment; on the basis of the constitution of the cell wall of the sweet tea, cellulose decomposition bacteria with efficient enzyme activity are screened and act on the sweet tea to damage the cell wall structure of the sweet tea, so that the release of active substances in the sweet tea is improved, the extraction rate of sweet tea flavone and sweet tea polyphenol is improved, and a green biological pretreatment technology is formed.

This invention provides a type of yeast called *Saccharomyces clavatum*, characterized in that the *Saccharomyces clavatum* is: *Saccharomyces clavatum* (… Saccharomycopsis fibuligera AMCC 32248, preservation number CCTCC NO: M 20251908. Enzyme activity phenotypic screening, carbon source utilization analysis, and quantitative enzyme activity detection showed that this strain possesses highly efficient β-glucosidase activity; further analysis of key genes related to β-glucosidase... BGL1 Specific functional sequences were extracted and identified. Enzyme production conditions were optimized using response surface methodology, resulting in a significant increase in enzyme activity compared to before optimization. In a simulated fermentation system, this strain significantly promoted the production of specific acids, esters, aldehydes, alcohols, and other volatile flavor compounds. Its enzyme production characteristics and flavor regulation capabilities provide a new technical solution for addressing issues such as low hydrolysis efficiency of glycosides and insufficient flavor release in fermentation systems, showing potential applications in the food, brewing, feed, and pharmaceutical industries.

The invention discloses a 5-(2-nitrophenyl)-2-phenyl-1, 10b-dihydro-5H-benzo [e] pyrazolo [1, 5-c] [1, 3] oxazine compound and application of the 5-(2-nitrophenyl)-2-phenyl-1, 10b-dihydro-5H-benzo [e] pyrazolo [1, 5-c] [1, 3] oxazine compound in a medicine for treating diabetes mellitus. The compound as shown in the following formula (1) is obtained through virtual screening in combination with experimental verification, and the compound has remarkable alpha-glucosidase inhibitory activity. In-vitro and in-vivo experiments prove that the compound effectively reduces the generation of glucose and delays the digestion and decomposition of starch by inhibiting the activity of alpha-glucosidase. The invention discloses the compound and the application thereof in the field of preparation of anti-diabetic drugs for the first time, the alpha-glucosidase inhibitory activity of the compound is high, and a new direction is provided for development of diabetes treatment drugs. Formula (1) below

The application discloses application of a hypoglycemic peptide in preparation of a medicine for inhibiting alpha-amylase and alpha-glucosidase activity. The hypoglycemic peptide is derived from Hericium erinaceus, and the amino acid sequence is AVFPSIVGRPR. The polypeptide is extracted and purified from Hericium erinaceus fruiting body protein, the amino acid sequence of one hypoglycemic peptide is determined by LC-MS / MS, the alpha-amylase inhibition rate is 71.86%±0.08% through hypoglycemic activity detection, and the alpha-glucosidase inhibition rate is 61.52%±0.15%, so the hypoglycemic peptide can be used as a natural hypoglycemic peptide and has a potential application prospect in the field of preparation of medicines for reducing blood sugar and the like.

The invention provides a Trichoderma reesei engineering bacterium for high yield of beta-glucosidase, which is characterized in that a Trichoderma reesei SN1 strain is used as a starting strain, and an expression cassette for knocking out a cre1 gene, a constitutive expression cassette of an aspergillus niger source bglA gene expressed by a promoter cbh1 and a constitutive expression cassette of a 130442 gene expressed by the promoter cbh1 are constructed in a genome of the Trichoderma reesei SN1 strain. Through the knockout of the cre1 gene, the overexpression of the aspergillus niger source bglA gene and the overexpression of the 130442 gene, the yield of the beta-glucosidase in the trichoderma reesei is improved synergistically. Glucose is taken as a raw material, a transglycosylation reaction can be catalyzed by directly utilizing a crude enzyme solution of trichoderma reesei engineering bacteria with high yield of beta-glucosidase to prepare beta-disaccharide, and the beta-disaccharide can be taken as an inducer for cellulase production; a crude enzyme liquid with higher cellulase activity and beta-glucosidase activity is obtained by supplementing an inducer and carrying out fed-batch fermentation.

The invention belongs to the technical field of tobacco strains, and particularly relates to a rhodotorula mucilaginosa H04 strain and application thereof in preparation of tobacco flavor. The rhodotorula mucilaginosa H04 provided by the invention has excellent aroma producing performance and can produce tobacco characteristic aroma substances such as alcohols, aldehydes, esters and pyrazines at high yield. The rhodotorula mucilaginosa H04 has good tolerance to nicotine, can still keep high growth activity under the condition that the nicotine concentration is as high as 8 mg / mL, and is suitable for being used for preparing tobacco flavor; the tobacco flavor prepared by the invention contains a plurality of natural aroma components, has good coordination with the original aroma of tobacco, and can significantly improve the aroma richness and comfort of tobacco products; the highest beta-glucosidase activity of the rhodotorula mucilaginosa H04 reaches 464.0 + / -24.4 U / mL, and the laccase activity reaches 60.4 + / -3.2 U / mL, so that the release of bound aroma precursor substances in tobacco is facilitated, and the aroma expression is enhanced.

The invention discloses a hesperetin derivative containing a thiosemicarbazone structure and application of the hesperetin derivative, the molecular formula of the compound is C18H19N3O5S, the biological activity determination result shows that the compound has good anti-oxidation and anti-alpha-glucosidase activity, the IC50 value of DPPH scavenging activity is 6.17 mu g / mL, and the activity of the hesperetin derivative is equivalent to that of a control agent vitamin C. The hesperetin derivative containing the thiosemicarbazone structure and the application of the hesperetin derivative containing the thiosemicarbazone structure have good anti-oxidation and anti-alpha-glucosidase activity. The IC50 value of ABTS clearance activity is 0.04 mu g / mL, and the activity is improved by 200 times compared with that of a control medicament vitamin E; the IC50 value of the alpha-glucosidase inhibitory activity is 4.53 [mu] g / mL, which is improved by 51 times compared with the activity of a control agent acarbose; the structural formula of the hesperetin derivative containing the thiosemicarbazone structure is shown in the specification.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis, a straw degradation agent, a preparation method, a straw degradation method and application. The invention provides a bacillus velezensis LLS, and the preservation number of the bacillus velezensis LLS is CGMCC (China General Microbiological Culture Collection Center) No.35634. The bacillus velezensis LLS provided by the invention is high in cellulase total activity, endo beta-1, 4-glucosidase activity, exo beta-1, 4-glucosidase activity, beta-1. 4-glucosidase activity, lignin peroxidase activity, laccase activity and manganese peroxidase activity, can tolerate low temperature, and can be used for preparing the bacillus velezensis LLS. The straw degradation effect is good under the low-temperature condition.

The invention provides saccharomycopsis fibuligera, which is characterized in that the saccharomycopsis fibuligera is saccharomycopsis fibuligera AMCC 32248, and the preservation number of the saccharomycopsis fibuligera is CCTCC (China Center for Type Culture Collection) NO: M 20251908. Enzyme activity phenotype screening, carbon source utilization analysis and enzyme activity quantitative detection show that the strain has efficient beta-glucosidase activity; furthermore, the key gene BGL1 of the beta-glucosidase is called, and a specific functional sequence is excavated. The enzyme production condition is optimized through a response surface method, and the enzyme activity is remarkably improved compared with that before optimization. In a simulated fermentation system, the strain can remarkably promote generation of volatile flavor substances such as specific acids, esters, aldehydes and alcohols, and a new technical scheme is provided for solving the problems of low hydrolysis efficiency of glycoside substances, insufficient flavor release and the like in the fermentation system due to the enzyme production characteristic and flavor regulation and control capability of the strain. The method has application potential in the fields of food, brewing, feed, medicine and the like.

This invention belongs to the field of biomedical technology, specifically disclosing a blood sugar-lowering probiotic and its application. The strain is Staphylococcus xylose (S. xylose). Staphylococcus xylosus. BC100, deposited on December 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37053, located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Compared with existing technologies, the fermentation broth of *Staphylococcus xylose* strain BC100 provided by this invention has the effect of inhibiting α-amylase and α-glucosidase activities. Furthermore, the fermentation broth of this strain combined with fruit enzymes exhibits even better inhibitory effects, particularly when combined with lemon enzymes and apple enzymes. This strain can be used to develop oral or other products for lowering blood sugar and has significant application value.

The invention belongs to the technical field of biological medicine, and relates to sargassum polysaccharide as well as a preparation method and application thereof. The uniform acidic polysaccharide WZSP-2 is separated and purified from the Weizhou Sargassum through hot water extraction, ethanol precipitation, protein and color removal, anion exchange column and gel column chromatography and other methods, and the physicochemical properties, monosaccharide composition and structural morphology of the Weizhou Sargassum are systematically characterized. In-vitro experiments show that the WZSP-2 has remarkable DPPH, ABTS and hydroxyl radical scavenging capacity and total reducing power, can effectively inhibit the activity of alpha-amylase and alpha-glucosidase, can be combined with glycocholate and taurocholate, and has a bacteriostatic effect on methicillin-resistant staphylococcus aureus (MRSA) at the same time. The sargassum polysaccharide provided by the invention integrates antioxidant activity, hypoglycemic activity, hypolipidemic activity and antibacterial activity, provides a new source for developing multifunctional natural products, and has a good application prospect in the fields of functional food, health care products and medical auxiliary materials.

The invention relates to the technical field of biology, in particular to a zymomonas mobilis recombinant strain for producing ethanol by taking cellobiose as a raw material and application of the zymomonas mobilis recombinant strain. On the basis of recombinant zymomonas mobilis expressing beta-glucosidase, an efficient cellobiose conversion system is constructed through the synergistic effect of promoter and signal peptide optimization and gene multi-copy regulation, the activity of extracellular beta-glucosidase is remarkably improved, cellobiose is accelerated to be hydrolyzed into glucose, and the activity of beta-glucosidase is improved. The yield of ethanol is promoted to be obviously improved. Based on the fact that no linear positive correlation exists between the activity of beta-glucosidase and the yield of ethanol, the bottleneck of cellulose degradation under high substrate concentration is solved through multi-element coordinated regulation and dynamic matching of enzyme activity and substrate metabolism, and the energy efficiency and industrial application value of biomass energy conversion are remarkably improved.