101 results about "Paclobutrazol" patented technology

The invention provides an anti-premature senility management method for closely-planted double-layer truncated cone macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The premature senility prevention management method comprises layered nutrition supply and flower and fruit protection management. The layered nutrition supply comprises the following steps: deeply applying a calcium magnesium phosphate fertilizer to the lower layer of soil greater than 60cm, and covering a root controller; organic fertilizers and biological agents are applied to 30-60 cm soil of the middle layer; a macroelement, humic acid and seaweed extract water-soluble fertilizer is applied to the 0-30 cm soil of the upper layer; the flower and fruit protection management comprises the steps that ABA and paclobutrazol are sprayed in the flower bud differentiation period; brassinolide and fluid boron are sprayed in the full-bloom stage; spraying forchlorfenuron and amino acid in a young fruit stage; nAA and monopotassium phosphate are sprayed in the fruit swelling period. Layered nutrition supply and flower and fruit protection management are carried out on the densely-planted double-layer truncated cone macadamia nut trees, premature senility of the densely-planted macadamia nut trees is prevented, branch development of the lower layer is promoted, the tree vigor is balanced, and the high-yield level is maintained.

The invention discloses a special leaf fertilizer for regenerative fragrant rice and application of the special leaf fertilizer. The special leaf fertilizer for the regenerated fragrant rice contains regulating substances such as triacontanol, gibberellin, diethyl aminoethyl hexanoate, pimacol, paclobutrazol and amino acid, and also contains microelement fertilizers such as zinc sulfate, ferrous sulfate, copper sulfate, manganese sulfate and ammonium molybdate. The special foliar fertilizer for the regenerated fragrant rice is appropriate in component and proper in proportion, and the yield of the regenerated fragrant rice can be effectively increased and the content of fragrant substances of the regenerated fragrant rice can be increased by applying the foliar fertilizer after first season fragrant rice is harvested. The special leaf fertilizer for the regenerated fragrant rice has the effects of increasing yield and aroma, and is beneficial for improving quality and efficiency of rice industry production.

The invention relates to the technical field of microalgae heterotrophic culture and biomass proliferation, in particular to a chlorella pyrenoidosa heterotrophic high-density proliferation method. The specific technical scheme is as follows: the heterotrophic high-density proliferation method of chlorella pyrenoidosa comprises the following steps: pre-culture and heterotrophic propagation of chlorella pyrenoidosa: inoculating a chlorella pyrenoidosa solution into a BG11 culture medium, and culturing for 3-4 days in a constant-temperature shaking table with the temperature of 25 + / -1 DEG C, the rotating speed of 200rpm and the light-dark ratio of 0h: 24h to obtain a primary culture chlorella solution; inoculating the primary culture algae liquid into a pre-sterilized and optimized heterotrophic culture medium, and carrying out expanding culture under the same culture condition for 3-4 days to obtain a secondary expanding culture algae liquid; (2) a heterotrophic high-density culture stage: regularly supplementing a nutrient culture medium containing high-concentration glucose, nitrate and phosphate by adopting an index fed-batch feeding strategy; other conditions are kept consistent during culture; and (3) an in-situ protein accumulation induction stage: when the chlorella pyrenoidosa reaches an exponential growth end stage, adding paclobutrazol into the system, and keeping other conditions consistent while keeping exponential fed-batch culture. According to the method disclosed by the invention, the biomass of the chlorella pyrenoidosa is increased by about 128.6% compared with that of the traditional batch culture, the protein content can reach 49.14%, meanwhile, the configuration of essential amino acids is improved, and the requirements of high-end biomass resource application are met. The method has the advantages of simplicity and convenience in operation and high production efficiency, and is suitable for the fields of high-quality protein extraction and related biomass production.

The invention discloses a water lily plant miniaturization treatment method which comprises the following steps: planting water lily fetal seedlings by using a pot sleeving method, recovering the seedlings with clear water for 15-20 days, applying paclobutrazol once every 6-9 days until new root systems grow out and the root systems and soil are staggered, and applying a 0.08-0.15 mL / L paclobutrazol suspending agent with 25% of effective components for three times in total. According to the method, the leaf area and the flower stem of the water lily can be obviously reduced, the flowering number is not influenced, and the plant growth condition is good. The method is easy to operate and convenient to use, can be widely applied to small-sized cultivation of water lily plants, and lays a foundation for production of small-sized water lily potted flowers suitable for family gardening.

The invention discloses a paclobutrazol hapten as well as synthesis and application thereof. The structure of the paclobutrazol hapten is shown as a formula I. Compared with 3-6 carbon chains, the synthetic method of the paclobutrazol hapten provided by the invention has the characteristics of mild reaction conditions, high yield, few reaction steps and capability of reducing the cost of raw materials. The paclobutrazol colloidal gold detection product provided by the invention has the characteristics of strong specificity, high sensitivity and the like, can realize accurate detection of paclobutrazol in a sample, and has no cross reaction with other drugs. # imgabs0 #

The invention belongs to the technical field of plant cultivation, and particularly relates to a wheat shallow-buried drip-irrigation uniform-sowing densification high-yield cultivation method and system, two main treatments of traditional sowing and uniform sowing are set, four different cultivation densities are set at the same time, and changes of wheat growth and yield under different treatments are observed; a drip irrigation tape laying mode of one pipe and two rows is adopted; two chemical control agents of chlormequat chloride and paclobutrazol * mepiquat chloride are sprayed in the three-leaf stage and the elongation stage of wheat, and the dosage of the chemical control agents is set to be three gradients; determining a chemical regulation and control scheme of the spring wheat under the close planting cultivation condition; under the condition of close planting, the control effect of close planting chemical control on wheat growth and yield is studied by adopting a split area test design, and the control effect of water and nitrogen control on wheat growth and yield is studied. According to the method, the planting density and the planting specification of a shallow-buried drip irrigation uniform sowing mode are defined, and a high-yield, high-quality and high-water-efficiency irrigation and fertilization scheme for wheat is determined.

The invention discloses a cultivation and harvesting method for improving the chicoric acid content of echinacea purpurea leaves, and the cultivation method comprises the following steps: applying an organic fertilizer, turning over, ridging in a field, and planting at a planting interval of 29-31 cm * 29-31 cm; in the plant growth process, hormone foliage spraying regulation and control are conducted twice, and the interval between two times of spraying is at least one week; the hormone is selected from paclobutrazol with the concentration of 1000 mg / L or 500 mg / L; in the later stage of the full-bloom stage each time, harvesting and harvesting overground parts, the harvesting time is 10 o'clock to 12 o'clock in the morning, and a distance is reserved at the base part; the content of chicoric acid in the harvested leaves is increased. According to the method, the content of chicoric acid in echinacea purpurea leaves is increased, growth is promoted, and plants are dwarf and strong by adjusting and controlling base fertilizer, planting spacing, harvesting in different months, harvesting in different time periods and hormone spraying in the cultivation process.

The invention belongs to the technical field of biology, and particularly discloses application of several compositions based on methyl carboline acid in stress resistance and growth promotion of plants. The invention solves the problems of single function, poor effect, large dosage, high cost and the like of the plant regulator in the market, and the methyl carboline acid, paclobutrazol, alginate oligosaccharide and diethyl aminoethyl hexanoate are compounded for use under a specific concentration, so that the growth of crops can be obviously promoted, and the yield and quality of the crops can be improved. The methyl carboline acid and the gamma-aminobutyric acid are compounded for use under a specific concentration, so that the influence of saline-alkali stress on the growth of crops such as wheat and cucumber can be remarkably relieved; the methyl carboline acid and the 5-aminolevulinic acid are compounded for use under a specific concentration, so that the growth of crops such as tomatoes and peanuts at a low temperature can be promoted. The stress-resistant and growth-promoting composition provided by the invention can be used for remarkably improving the self resistance of plants under a proper concentration, effectively promoting the growth and development of the plants and improving the yield and quality of crops.

The invention relates to the field of microorganisms, in particular to a Kutta gibsonii B7 for degrading paclobutrazol and application of the Kutta gibsonii B7. The Kutta gibsonii B7 is preserved in the China General Microbiological Culture Collection Center on October 23, 2024, and the preservation number is CGMCC No.32305. The Kutta gibsonii B7 can grow in a liquid culture medium containing the paclobutrazol, and the Kutta gibsonii B7 can be used for degrading the paclobutrazol. The content of paclobutrazol in the culture medium can be reduced, and the toxic effect of paclobutrazol on thalli is relieved; 95 mg / L paclobutrazol can be degraded in 9 days, and the degradation rate can reach 74%; a degradation product obtained after paclobutrazol is degraded has obviously weakened stress and phytotoxicity effects on plants, and is low in cost compared with physical and chemical cost and free of secondary pollution; the strain can be used for preparing a microbial agent for degrading paclobutrazol in soil, can obviously relieve the apical inhibition effect of paclobutrazol on crops, and has a relatively great application prospect.

The invention discloses a cultivation method for controlling tips and promoting flower bud differentiation of syzygium samarangense, and belongs to the technical field of tip control cultivation of syzygium samarangense. The method comprises the following steps: slightly shearing 70-80 days before flower forcing in the wax apple production period, applying a phosphorus-potassium-silicon mixed fertilizer in holes on the ground, and applying the fertilizer once every 15 days, totally twice; then a mixed aqueous solution of potassium phosphite and paclobutrazol is sprayed to leaf surfaces and drips water, spraying is conducted once every 15 days, and spraying is conducted 2-3 times in total; after spraying is conducted for 5-7 days, closed girdling is conducted, after 40-45 days, a mixed aqueous solution of auxin and a plant nutrition regulator is used for spraying, spraying is conducted once every 15 days, and spraying is conducted 1-2 times in total. The silicon fertilizer is mixed and added in the fertilizer and water management period to assist in increasing the absorption and utilization rate of the phosphorus-potassium fertilizer; besides, through the synergistic effect of tip control, trunk girdling and a mixed aqueous solution of auxin and a plant nutrition regulator, syzygium samarangense plants are stable, flower bud differentiation is promoted, meanwhile, the method has the characteristics of disease resistance, disease prevention, high flower forcing and flowering rate and large inflorescence amount, and the important effects of annual flowering and fruiting and promotion of syzygium samarangense industry synergy can be achieved; the method has the advantages of simple operation process, high efficiency, stability, few use times and labor cost saving.

The invention discloses a planting method for delaying flowering and fruit setting of citrus, and belongs to the technical field of citrus planting. The planting method comprises the following steps that S1, autumn shoots are promoted to sprout and mature, before the autumn shoots sprout, existing branches are trimmed, nitrogenous fertilizer, phosphate fertilizer and potash fertilizer are applied, and 0.2-0.4% monopotassium phosphate and 200-300 ml / L paclobutrazol are sprayed after new shoots are self-trimmed; s2, spring shoot germination inhibition: before spring shoot germination, rain sheltering and water control are carried out, and soil drought is kept; s3, sprouting of early summer shoots is promoted, before sprouting of the early summer shoots, existing branches are trimmed, and nitrogenous fertilizer, phosphate fertilizer and potash fertilizer are applied; s4, fruit retention: spraying a fruit retention agent during the end-flowering period and after the first physiological fruit drop period. The method can delay the maturing and marketing time of citrus, and prolongs the fresh fruit supply period by about 90 days compared with a conventional planting mode.

The utility model relates to the technical field of paclobutrazol production, and discloses a pretreatment device convenient to clean and maintain for paclobutrazol production, which comprises a support plate, a lifting rotating replacement part and a rotating cleaning part, support legs are fixedly mounted on the outer wall of the bottom of the support plate, and support base plates are fixedly mounted at the bottoms of the support legs; the lifting rotating replacement part is arranged at the top of the supporting plate; and the rotary cleaning part is arranged at the top of the supporting plate. The cleaning fur is in close contact with the barrel wall, dirt is removed through friction, the cleaning stirring blocks stir at the barrel bottom to form vortexes and disturbances, the barrel wall and the barrel bottom can be comprehensively and deeply cleaned through the combination mode, residual materials and dirt are effectively removed, the cleanliness of the pretreatment stirring barrel is guaranteed, and the cleaning efficiency is improved. Through cooperative operation of the electric telescopic rod and the rotating motor, rapid switching and positioning of the stirring device and the cleaning device are achieved, complex manual dismounting and remounting are not needed, and time and labor cost are saved.

This invention relates to the field of soil treatment technology, specifically a method and its application for reducing compound fungicides in soil-plant systems by combining nano-silica and nitrification inhibitors. Nitrification inhibitors are used as a nitrogen management measure, and the application of nanomaterials influences soil abiotic properties and bacterial communities to drive the degradation of paclobutrazol and carbendazim. The application of nitrification inhibitors (DCD) produces multiple effects: significantly reducing carbendazim residues in soil-plant systems, paclobutrazol residues in soil, and NO3 in plants. ‑ -N and promote the content of soluble sugars and proline in plants. DCD reduces the activity of the soil electron transport system and β-glucosidase activity, while the addition of nano-SiO2 can mitigate this negative effect. Nano-SiO2 selectively enhances the performance of DCD in reducing compound fungicide residues in pak choi and reducing paclobutrazol residues, but hinders the degradation of carbendazim in pak choi.

The invention relates to an ionic liquid for capping cotton, an emulsion in water as well as a preparation method and application of the ionic liquid and the emulsion in water, and belongs to the technical field of plant growth regulation. The ionic liquid disclosed by the invention comprises paclobutrazol and salicylic acid, and the molar ratio of the paclobutrazol to the salicylic acid is 1: (1-1.5); the method comprises the following steps: mixing the ionic liquid with an emulsifier, an anti-freezing agent and water, and uniformly mixing by using a high-speed homogenizer to obtain an emulsion in water product for cotton capping. The ionic liquid disclosed by the invention is relatively good in water solubility, has capping and high-temperature-resistant activity, and can be directly processed into an emulsion in water. Stem and leaf treatment is performed in the early flowering stage of cotton, the effective utilization rate is high, capping is stable, and the high-temperature resistance of cotton can be improved. Meanwhile, the ionic liquid and the emulsion in water are simple in preparation process and convenient to apply.

The utility model relates to the technical field of paclobutrazol production, in particular to a mixing device for production of a plant growth regulator paclobutrazol, which comprises a stirring tank, a feeding hopper is fixedly mounted at the upper end of the stirring tank, a base is fixedly mounted at the lower end of the stirring tank, a discharging pipe is fixedly mounted on the lower side of the stirring tank, and a feeding hopper is fixedly mounted at the upper end of the stirring tank. The discharging pipe is located at the center of the lower side of the stirring tank, a first motor is fixedly installed at the upper end of the stirring tank, a stirring assembly is arranged in the stirring tank, and a conveying assembly is arranged at the lower end of the discharging pipe. The first motor of the output shaft drives the rotating shaft and the stirring rod to rotate, the stirring rod swings on the support with the circular shaft as the axis, meanwhile, the rotating shaft rotates to drive the gear and the universal coupling to rotate, and the gear further drives the universal coupling and the stirring rod to rotate at the rear end of the rotating shaft through the gear ring. Therefore, rotary stirring of the rotating shaft and the stirring rod and rotary stirring of the axis of the stirring rod are achieved, and the uniformity and efficiency of material mixing are improved.

The invention belongs to the field of liquid fertilizers, and relates to a process for promoting the growth of commercial crops by using a lincomycin finished product liquid fertilizer, which comprises the following steps: diluting lincomycin dry powder according to a mass ratio of 1: 100 to obtain a lincomycin finished product liquid; the fertilizer is prepared from a modified accelerant, urea, monopotassium phosphate, calcium superphosphate, a calcium magnesium phosphate fertilizer, potassium chloride, zinc sulfate, borax, an iron fulvate solution, paclobutrazol and lincomycin fermentation liquor. Dissolving urea, potassium chloride, zinc sulfate and the iron fulvic acid solution in water, and uniformly mixing to prepare a nutrient solution; performing 200-DEG C high-temperature treatment on zeolite with the fineness of 200 meshes, and spraying the nutrient solution onto the zeolite; airing or drying the zeolite until the water content of the zeolite is reduced to be not higher than 5%; and uniformly mixing the residual chemical fertilizer with the modified accelerant to obtain the lincomycin finished product liquid fertilizer. The lincomycin finished product liquid fertilizer provided by the invention can improve the growth of crops.

The invention belongs to the technical field of rice planting, and discloses a rice lodging-resistant strength method which comprises the following steps: S1, selecting a treatment agent: selecting paclobutrazol as an inhibitor for internode elongation; s2, preparing a pesticide: weighing paclobutrazol and clear water according to the rice planting area, and uniformly mixing to obtain a paclobutrazol solution; wherein the dosage of the paclobutrazol is 225 g / hm < 2 > to 300 g / hm < 2 > according to pure product calculation; the use amount of the clear water is 225kg / hm < 2 > or 30L / hm < 2 >; s3, spraying a paclobutrazol solution: spraying the paclobutrazol solution 3-5 days before the tillering period of the rice and jointing. The problem that rice is prone to lodging under the conditions of partial nitrogen, high yield and poor variety lodging resistance is solved, and the yield and quality of the rice are improved.

The application discloses a mango shoot control and flower promoting PGRs-multifunctional structured molecular additive synergistic composition and application thereof, and belongs to the technical field of mango growth regulation. The composition is composed of five sub-compositions used in stages, each of the sub-compositions containing a plant growth regulator, a nutrient element and a specific structured molecular additive; the structured molecular additive is combined with the plant growth regulator through physical embedding, electrostatic adsorption or hydrophobic interaction. The application also provides an application method of the composition, including two stages of soil shoot control and leaf surface regulation. Through construction of an intelligent delivery system, the application realizes environment-responsive release, slow release and targeted delivery of active ingredients, effectively solves technical bottlenecks of poor stability, low targeting and high residual risk of traditional plant growth regulators, can accurately regulate the growth cycle of mangoes, significantly improves yield and quality, and greatly reduces pesticide residues and environmental load.

The utility model discloses a pressure-adjustable reaction kettle for paclobutrazol production, which comprises a kettle body, a servo motor is mounted in the center of the top of the kettle body, the output end of the servo motor is connected with a driving rotating shaft, and a bevel gear I is mounted at the tail end of the driving rotating shaft; the gear box is connected to the lower part in the kettle body through a transverse plate; the driven rotating shaft is connected to the surface of the gear box through a bearing sleeve and extends to the inner wall of the kettle body, and a bevel gear I meshed with the bevel gear II is mounted at one end of the driven rotating shaft; and the cleaning plate is mounted at the tail end of the driving rotating shaft, and a cleaning brush is arranged at the bottom of the cleaning plate. According to the stirring device disclosed by the utility model, the bevel gear I, the bevel gear II and the driven rotating shaft are adopted, so that the auxiliary stirring rod fully stirs impurities which are gathered and precipitated at the bottom so as to improve the overall stirring speed, and the cleaning plate is adopted to clean the impurities accumulated on the surface of the cleaning plate so as to reduce the occurrence of the condition that the filter plate is blocked due to the accumulation of the impurities.

The invention relates to a hybridoma cell strain secreting a mepiquat chloride monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 17, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46517. The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity to mepiquat chloride, the IC50 value of the mepiquat chloride is 4.981 ng / mL, structural analogues and functional analogues of the mepiquat chloride, such as chlormequat chloride, paclobutrazol and uniconazole, have no cross reaction, the cross reaction rate is smaller than 1%, and the monoclonal antibody secreted by the hybridoma cell strain can be used for preparing the mepiquat chloride monoclonal antibody. Therefore, low-concentration mepiquat chloride can be accurately detected.

The invention relates to the technical field of grape cultivation, in particular to a method for delaying vitis heyneana germination, which comprises the following steps: in the first ten-day period of March, spraying a treating agent on vitis heyneana, and the treating agent comprises the following components: maleic hydrazide, ethephon, paclobutrazol, morning glory extract and tobacco extract. The method is easy to operate, low in cost, green and safe, germination of vitis quinquangularis is delayed by spraying the pesticide, high-incidence time of downy mildew is avoided in the florescence of the vitis quinquangularis, and meanwhile the growth quality of the vitis quinquangularis is effectively improved.

The application provides a method for preventing early decline of densely-planted double-layered circular table-shaped macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The method for preventing early decline comprises hierarchical nutrient supply and flower and fruit protection management. The hierarchical nutrient supply comprises: deep application of calcium, magnesium and phosphorus fertilizer in the lower layer of more than 60cm soil, and covering with a root control device; application of organic fertilizer and biological bacteria agent in the middle layer of 30-60cm soil; and application of a large amount of elements, humic acid and seaweed extract water-soluble fertilizer in the upper layer of 0-30cm soil. The flower and fruit protection management comprises: spraying ABA and paclobutrazol during flower bud differentiation period; spraying brassinolide and fluid boron during full flowering period; spraying forchlorfenuron and amino acid during young fruit period; and spraying NAA and potassium dihydrogen phosphate during fruit swelling period. The application supplies nutrients hierarchically and protects flowers and fruits for densely-planted double-layered circular table-shaped macadamia nut trees, prevents early decline of the densely-planted macadamia nut trees, promotes the development of lower branches, balances tree vigor and maintains high yield.

The invention relates to the technical field of lily genetic breeding, in particular to a stable genetic transformation method for infecting lily scales without depending on a tissue culture technology. According to the method, a stable genetic transformation system without tissue culture of the lily scales is established through an agrobacterium tumefaciens mediated method, and different agrobacterium tumefaciens strains, infection conditions, infection time and the infection efficiency of paclobutrazol (PBZ) on the lily scales are systematically explored. The defect that a genetic transformation system needs to use a sterile tissue culture technology to induce calluses is overcome. By optimizing key parameters of a tissue culture-free genetic transformation system of the lily scales, a reference basis is provided for subsequently establishing an efficient and stable genetic transformation system.

This invention discloses a hapten, an artificial antigen, and their applications for detecting paclobutrazol. The paclobutrazol hapten prepared by this invention retains the characteristic structure of paclobutrazol to the greatest extent possible. The paclobutrazol antibody prepared using the paclobutrazol hapten of this invention exhibits high potency and specificity, with a half-maximum inhibitory concentration (WMC) of 0.31 ng / mL and a linear range of 0.03–19.63 ng / mL. Furthermore, it shows no cross-reactivity with other structural and functional analogs such as uniconazole and hexaconazole. This invention simplifies the synthesis steps of the paclobutrazol hapten, and the method for preparing the paclobutrazol artificial antigen and its antibody is simple and low-cost. It also establishes a more efficient immunoassay method, capable of meeting the requirements for large-scale on-site sample testing and has broad application prospects.

The invention discloses a culture medium for acer elegans tissue culture, and belongs to the technical field of plant tissue culture. The culture medium comprises a double system of a germination culture medium with the pH value of 5.5-5.8 and a rooting culture medium with the pH value of 5.8-6.3. Wherein the germination culture medium takes potassium nitrate, calcium nitrate terahydrate, magnesium chloride hexahydrate and monopotassium phosphate as basic components, an ammonium-free solution system is innovatively adopted, a redox buffer system formed by ascorbic acid and cysteine is combined, and plant growth regulators such as TDZ and gibberellin are added. The rooting culture medium promotes root development through potassium nitrate and calcium nitrate tetrahydrate in a specific ratio in cooperation with IBA and phloroglucinol, and cane sugar is adopted as a carbon source and supplemented with activated carbon. By strictly controlling the molar ratio of Ca to Mg (2-2.5: 1) and the synergistic effect of paclobutrazol and other components, the browning occurrence rate of explants and the occurrence rate of glass seedlings are greatly reduced, and reliable technical support is provided for acer elegans germplasm resource preservation and industrial seedling raising.

This invention discloses a method for improving the aroma of regenerated fragrant rice. Belonging to the field of crop cultivation technology, this method significantly increases the content of the aroma compound 2-acetyl-1-pyrroline (2AP) in regenerated fragrant rice by simultaneously applying paclobutrazol or uniconazole (two plant growth regulators) during the tillering fertilizer application for the first-season fragrant rice, applying urea as a bud-promoting fertilizer before harvesting the first-season fragrant rice, and controlling the harvesting timing and stubble height of the first-season fragrant rice. Simultaneously, it can stabilize or increase the yield of fragrant rice, achieving stable or increased yield while improving the aroma. This invention is beneficial for improving the rice quality of regenerated fragrant rice and promoting its production.

The present application relates to the Malus domestica Borkh cultivation technical field, specifically, it relates to a kind of greenhouse container seedling cultivation method for improving the ornamental of Malus domestica Borkh. Including the following steps, selecting the root control container of air permeability, preparation special cultivation substrate;Strong seedling is planted in root control container, is placed in shady environment and is carried out with slow seedling;Control day and night temperature, illumination and water nutrient;Water and fertilizer integration system is used to irrigation and fertilization;Use physical method of pinching, pull branch, ring cutting, and combine with the foliar spray of paclobutrazol;Through the coupling application of accurately regulated day and night temperature difference and photoperiod management, water and fertilizer integration and synergistic drought stress, and the organic combination of physical shaping and chemical control, the synergistic effect between temperature, illumination, water, nutrient and other factors is generated, so that the beneficial effects of inhibiting overgrowth, promoting branching, efficient inducing flower bud differentiation and shaping compact plant type are realized synchronously on the whole, and the ornamental quality and growth consistency of Malus domestica Borkh container seedling are improved.

This invention discloses a composition for prolonging the flowering period of durian and a method for regulating the flowering period of durian. The composition includes: a first spray agent, consisting of the following components in the indicated weight ratios: 5-20g of cytokinin-like substances; 1-5g of gibberellin-like substances; 10-30g of polyamine-like substances; 5-15g of boron source; 100-300g of energy-regulating components; 1-5g of slow-release carrier and penetration-promoting components; and water to 1000g; and a second spray agent, consisting of the following components in the indicated weight ratios: 20-50g of flowering rhythm-regulating components, which are composed of 5-10g of paclobutrazol, 3-8g of uniconazole, and 0.5-2g of chlorpyrifos; 2-5g of slow-release carrier and penetration-promoting components; and water to 1000g. This invention, through the combined design of the first spray agent (flowering promoter) and the second spray agent (flowering control), transforms the initiation of durian flower buds from a "single trigger" to a "controllable, phased trigger," thereby prolonging the flowering period while maintaining or even improving fruit-setting capacity.

The invention relates to the technical field of malus spectabilis seedling cultivation, in particular to a greenhouse container seedling cultivation method for improving the ornamental value of North American malus spectabilis. Comprising the following steps: selecting an air-permeable root control container, and preparing a special culture medium; the robust seedlings are planted in a root control container, and the container is placed in a shading environment for seedling recovery; controlling day and night temperature, illumination and water nutrients; a water and fertilizer integrated system is adopted for irrigation and fertilization; physical methods of pinching, branch pulling and girdling are applied, and paclobutrazol is sprayed on leaf surfaces; through precise regulation and control day and night temperature difference and photoperiod management, coupling application of water and fertilizer integration and synergistic drought stress and organic combination of physical shaping and chemical regulation and control, a synergistic interaction effect is generated among factors such as temperature, illumination, moisture and nutrients; therefore, the beneficial effects of inhibiting spindling, promoting branching, efficiently inducing flower bud differentiation and shaping compact plant types are synchronously achieved on the whole, and the ornamental quality and growth consistency of the North American malus spectabilis container seedlings are improved.

The invention discloses an application and a method of a BcCGA1. A gene in regulating and controlling the content of ascorbic acid in a plant. A large number of experimental studies find that the BcCGA1. A gene plays an important role in regulating and controlling the ascorbic acid content of the plant, specifically, the ascorbic acid content of the plant can be increased by reducing the expression quantity of the BcCGA1. A gene, and the expression quantity of the BcCGA1. A gene is influenced by the gibberellin content. On the basis, the invention provides a reagent, namely paclobutrazol, for increasing the ascorbic acid content of the plants, and an application method is designed according to the reagent, the method can effectively increase the ascorbic acid content of the plants under the weak light condition, and is simple, convenient, wide in application range, low in applied paclobutrazol content and low in influence on the environment; popularization and application are facilitated.