192 results about "Exponential growth" patented technology

Private Multimedia Network (PMN) complements, and is an improved alternative to digital videoconferencing and multimedia delivery systems. PMN's desktop and meeting room delivery system is designed to support the exponential growth of enterprise team-based initiatives. PMN provides “one-stop-shopping” for the full multimedia rubric. It delivers user-friendly control and cost / effective TV and broadcast quality videoconferencing and other multimedia services to organizations with “critical mass” campuses and building complexes. Though digital systems dominate the videoconferencing marketplace, PMN's hybrid digital / analog architecture has no digital peer in breadth or quality of service within or between campuses. The novel architecture leverages advances in analog video short-haul technology, digital long-haul technology, and telephony audio and control technology to deliver four-level multimedia services: 1) premise; 2) campus; 3) multi-site; and 4) ubiquitous (any site with ITU compatible multimedia equipment (e.g., videoconferencing) and communication links). On balance, the price / performance afforded by PMN's centralized Telco-based control and audio delivery combined with its decentralized broadcast quality video distribution raise videoconferencing and other multimedia services to a new level of ubiquity. Just as telephones and PC LANs, PMN delivers expensive Boardroom and mobile cart videoconferencing capabilities to every desktop via existing multimedia wall plates. The key phases for this invention are: Lip-synchronization across differing network communication links and protocols; Ubiquitous multimedia service; Cost / effective room and desktop deployment; Telco control and audio; Broadcast quality video; Isochronous Quality; Centralized control and distributed operation; and Interoperable architecture.

A system and method of metadata search ranking is disclosed. The present invention utilizes a combination of popularity and / or relevancy to determine a search ranking for a given search result association. Given the exponential growth rate currently being experienced in the Internet community, the present invention provides one of the few methods by which searches of this vast distributed database can produce useful results ranked and sorted by usefulness to the searching web surfer. The present invention permits embodiments incorporating a Ranking System / Method (0100) further comprising a Session Manager (0101), Query Manager (0102), Popularity Sorter (0103), and Association (0104) functions. These components may be augmented in some preferred embodiments via the use of a Query Entry means (0155), Search Engine (0156); Data Repository (0157), Query Database (0158), and / or a Resource List (0159).

A system and method of distributed metadata searching is disclosed. The present invention permits an extension of the searching and retrieval functions of existing Internet web search engines by utilizing computational resources embodied in user computer systems and search browsers. By distributing the searching and scanning functions to the user level, the present invention reduces the computational and communications burden on Internet web search engines and crawlers, resulting in lower computational resource utilization by Internet search engine providers. Given the exponential growth rate currently being experienced in the Internet community, the present invention provides one of the few methods by which complete searches of this vast distributed database may be performed. The present invention permits embodiments incorporating a Search Manger (1001) further comprising a Service Results Manager (1013), User Profile Database (1012), Service Manager(1013), and Service Database (1014); a Light Weight Application SCANNER (1002); and a Search Engine (1008). These components may be augmented in some preferred embodiments via the use of a Search Browser (1003), Internet Communications (1004); Web Site(s) (1005), Web Crawler(s) (1006), and a Repository Database (1007).

The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions encoding an enzyme associated with growth-coupled production of succinate when an activity of the enzyme is reduced, whereby the one or more gene disruptions confers stable growth-coupled production of succinate onto the non- naturally occurring microorganism. Also provided is a non-naturally occurring microorganism comprising a set of metabolic modifications obligatory coupling succinate production to growth of the microorganism, the set of metabolic modifications comprising disruption of one or more genes selected from the set of genes comprising: (a) adhE, ldhA; (b) adhE, ldhA, acka-pta; (c) pfl, ldhA; (d) pfl, ldhA, adhE; (e) acka-pta, pykF, atpF, sdhA; (f) acka-pta, pykF, ptsG, or (g) acka-pta, pykF, ptsG, adhE, ldhA, or an ortholog thereof, wherein the microorganism exhibits stable growth-coupled production of succinate. Additionally provided is a non-naturally occurring microorganism having the genes encoding the metabolic modification (e) acka-pta, pykF, atpF, sdhA that further includes disruption of at least one gene selected from pyka, atpH, sdhB or dhaKLM; a non-naturally occurring microorganism having the genes encoding the metabolic modification (f) ackA-pta, pykF, ptsG that further includes disruption of at least one gene selected from pykA or dhaKLM, or a non-naturally occurring microorganism having the genes encoding the metabolic modification (g) ackA-pta, pykF, ptsG, adhE, ldhA that further includes disruption of at least one gene selected from pykA or dhaKLM. The disruptions can be complete gene disruptions and the non-naturally occurring organisms can include a variety of prokaryotic or eukaryotic microorganisms. A method of producing a non-naturally occurring microorganism having stable growth-coupled production of succinate also is provided. The method includes: (a) identifying in silico a set of metabolic modifications requiring succinate production during exponential growth, and (b) genetically modifying a microorganism to contain the set of metabolic modifications requiring succinate production.

Disclosed is a power saving mode control system (200) and method in a wireless portable Internet system. Stations in the sleep mode are grouped by aligning listening intervals of the stations which enter the sleep mode in the power saving management system wherein the sleep interval for receiving no traffic data is exponentially increased. Therefore, the sleep mode of the grouped subscriber stations are easily managed, and power saving efficiency is enhanced and system complexity is lowered by easily and quickly detecting data states provided to the corresponding stations.

The invention provides a non-naturally occurring microorganism having one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory coupling 3-hydroxypropionic acid production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby the one or more gene disruptions confers stable growth-coupled production of 3-hydroxypropionic acid onto the non-naturally occurring microorganism. Also provided is a non-naturally occurring microorganism comprising a set of metabolic modifications obligatory coupling 3-hydroxypropionic acid production to growth of the microorganism, the set of metabolic modifications having disruption of one or more genes including: (a) the set of genes selected from: (1) adhE, ldhA, pta-ackA; (2) adhE, ldhA, frdABCD; (3) adhE, ldhA, frdABCD, ptsG; (4) adhE, ldhA, frdABCD, pntAB; (5) adhE, ldhA, fumA, fumB, fumC; (6) adhE, ldhA, fumA, fumB, fumC, pntAB; (7) pflAB, ldhA, or (8) adhE, ldhA, pgi in a microorganism utilizing an anaerobic β-alanine 3-HP precursor pathway; (b) the set of genes selected from: (1) tpi, zwf; (2) tpi, ybhE; (3) tpi, gnd; (4) fpb, gapA; (5) pgi, edd, or (6) pgi, eda in a microorganism utilizing an aerobic glycerol 3-HP precursor pathway; (c) the set of genes selected from: (1) eno; (2) yibO; (3) eno, atpH, or other atp subunit, or (4) yibO, atpH, or other atp subunit, in a microorganism utilizing a glycerate 3-HP precursor pathway, or an ortholog thereof, wherein the microorganism exhibits stable growth-coupled production of 3-hydroxypropionic acid. The disruptions can be complete gene disruptions and the non-naturally occurring organisms can include a variety of prokaryotic or eukaryotic microorganisms. A method of producing a non-naturally occurring microorganism having stable growth-coupled production of 3-hydroxypropionic acid is further provided. The method includes: (a) identifying in silico a set of metabolic modifications requiring 3-hydroxypropionic acid production during exponential growth, and (b) genetically modifying a microorganism to contain the set of metabolic modifications requiring 3-hydroxypropionic acid production.

Via real time online bandwidth measurement, this method confirms the threshold value ssthresh. When the congestion window (CW) is less than a half of the threshold, the rule of exponent gain is followed. Otherwise CW gains each time a value of a half of difference of the threshold and CW (ssthresh-cwnd) to approach gradually the threshold. Until the difference is less than the regulation factor phi (ssthresh / 2>= phi >=2). Then CW is set as the threshold value to enter the stage of avoiding congestion. This invention can reduce the happening of loss of several texts in a window at the slowly start stage, reduce the package loss rate and raise the net transferring ability.

A process for reducing the resources employed in real time to communicate a message between related nodes that are separated by multiple degrees of separation in a social network. At least a portion of the shortest path for the multiple degrees of separation between at least two related nodes in a social network is determined out of band prior to the initiation of a process to communicate between the related nodes. By pre-processing at least a portion of the degrees of separation for the shortest path between the nodes, the actual resources employed in real time to calculate the entire shortest path can be reduced. Typically, approximately fifty percent or more of the shortest paths for the degrees of separation between related nodes in the social network are pre-processed. Since the amount of resources for determining the shortest path for each degree of separation can exponentially increase with each degree, the pre-processing of a portion of the degrees of separation along a shortest path can significantly reduce the resources required in real time to complete the determination of the shortest path. Also, if a common intermediate node is identified in the pre-processing of the shortest paths for two nodes in the social network, the intermediate shortest paths can be stored for reuse as a complete shortest path between these two nodes,

This invention is a method about frequency multiplex. We employ numbers of sub-carriers whose frequency spectrum are overlapped each other to modulate the transmitted data sequences, and then we get the complex modulated signals. In the receiver point, we make use of one mapping between the overlapped signals' spectrum and the transmitted data sequences to detect the transmitted data sequences. By overlapping the adjacent sun-carriers, this invention can greatly increase the spectrum efficiency, and the more the multiplicity, the higher the spectrum efficiency.Furthermore, when the spectrum efficiency increases, the needed number of signal levels for this invention will not have exponential growth, just linear growth in stead, so the requirement for system linearity can be decreased greatly.

The invention discloses an implementation method of safe batch configuration of a Wi-Fi Internet of Things system, a master device in the Wi-Fi Internet of Things is configured through a safe manner, and then authentication and configuration are carried out on other devices in the Wi-Fi Internet of Things by the master device through a point-to-multipoint manner; the master device utilizes two different MAC (Media Access Control) addresses and synchronously supports two working modes including Wi-Fi Soft-AP and Wi-Fi STA; a mobile terminal of a user carries out initial configuration on the master device of the Wi-Fi Internet of Things in the Wi-Fi Soft-AP mode; and the master device is connected to other devices in the same group through the other Wi-Fi function to help other devices to complete agent initial configuration. According to the implementation method, a large amount of Wi-Fi Internet of Things devices are completed through a form of exponential growth, privacy data security is guaranteed and a large amount of manual cost can be saved.

The first data flow is buffered at a receiver, and the buffer contents are scanned for metadata. Where metadata are found indicating a second data flow which has not yet arrived, the system enters a stalling phase during which the length of any silent periods in the first data flow are stretched. As the point in the first data flow at which the second data flow is necessary gets closer, the factor by which silent periods are stretched increases exponentially. Once the expected second data flow in fact arrives, playback of two data flows is accelerated by compressing silent periods so as to clear the backlog of additional data that built up in the buffer during the stalling phase.

A method and structure for predicting semiconductor product costs at a fabricator entailing a storage medium which includes a database of historical critical dimensions and historical critical groundrules correlated to cost functions at the fabricator. The user interface has user inputs for new design parameters and new critical groundrules associated with a new device to be produced at the fabricator and a computer adapted to receive the user inputs, extract data from the storage medium, and compute semiconductor costs for the new device. The historical critical dimensions and the new critical dimensions are gate dimensions and the new critical dimensions are smaller than the historical critical dimensions. This device includes a future technology generation. Fabrication hardware and fabrication methods for producing the future technology generation are unknown and the relationships comprise base models and models that include options. The relationship comprise models that illustrate that costs increase exponentially as the historical critical dimensions and the historical critical groundrules are reduced.

A composition comprising a culture supernatant from a late-exponential growth phase of a batch-cultivation process for a probiotic such as LGG, for use in the treatment or prevention of infection by a pathogen such as C. sakazakki.

The invention relates to a thermal error modeling method based on gray linear regression. The method comprises the following steps that first, on the basis of a gray thermal error model, a linear equation is introduced, a gray linear regression combination model is constructed; second, a least square method is used for solving a gray linear regression combination model parameter; third, the gray linear regression model is used for thermal error prediction; fourth, a BP nerve network is used for amending combination model residual errors, and prediction accuracy is improved. According to the method, the shortcoming that a linear regression model does not have exponential growth and cannot describe linear changing trend easily, and a gray thermal error model does not have a linear factor can be overcome, good capacity for solving linear and nonlinear problems is achieved, good effect is achieved for thermal error prediction on an accurate horizontal type machining center is achieved, linear factors and nonlinear factors of thermal error data are considered, the shortcoming of an original single gray model is overcome, and an accurate thermal error prediction value and high fitting degree are acquired.

The invention belongs to the field of microalgae biotechnologies, and particularly relates to a method for synthesizing astaxanthin by inducing chlorella vulgaris by using plant hormones and iron ions. The method specifically comprises the steps of: activating and culturing the chlorella vulgaris in a liquid culture medium to obtain a synchronously growing chlorella vulgaris solution for later use; and taking the chlorella vulgaris solution growing to an exponential growth phase, adding the plant hormones and the iron ions in the chlorella vulgaris solution, replenishing a carbon source and a nitrogen source for culturing, ending the culturing until the accumulation of the astaxanthin in chlorella vulgaris cells is maximum (the accumulation of the astaxanthin is determined by adopting a liquid chromatography during sampling), and harvesting cell disruption and extracting the astaxanthin. The method is simple and easy to operate and low in cost, and is capable of remarkably increasing the yield of the astaxanthin, thereby greatly increasing the efficiency of producing the astaxanthin by the chlorella vulgaris.

A method for delivering a gas to a site comprising placing cells having gas vesicles under conditions that induce the cells to float to a surface of an aqueous medium, harvesting the cells from the surface of the medium, lysing the cells, separating the gas vesicles from the lysed cells, crosslinking the gas vesicles with a crosslinking agent, loading a gas into the gas vesicles, and placing the gas vesicles such that the gas is delivered to the site. Harvesting gas-vesicle-containing cells is achieved by placing the cells under conditions that induce the cells to rise to the surface of an aqueous medium-such as darkness, exponential growth stage, flocculation, or dissolved gas flotation-then collecting the cells from the surface of the medium. Gas vesicles are isolated by lysing the cells and separating the gas vesicles from the lysate. Once the gas vesicles are isolated, they can be modified, such as by crosslinking with glutaraldehyde.

The invention discloses a delta-sigma modulator based on the digital-noise coupling technology. The structure of a traditional noise coupling delta-sigma modulator is improved, and the output of a quantizer is divided into an MSB part and an LSB part. The MSB part is directly fed back to the input end of the modulator; the LSB part is fed back to the input of the quantizer after digital-to-analogue conversion; then the MSB part and the LSB part are recombined after digital post-processing. The digital-noise coupling technology can fully represent the performance advantage of the high-precision quantizer, the problem of the exponential growth of the matching logic complexity of dynamic components is avoided, the logic time delay of introduction of a digital delta-sigma remodulation scheme provided by formers is also avoided, the quantizing noise leakage caused by mismachining between analog and digital noise transfer functions is further restrained, and therefore the requirement for design of an operational amplifier is lowered, implementation is easy, and the modulator is very suitable for low-power dissipation and broadband application.

The invention discloses a signal combination coding-based DNA ligation sequencing method, which relates to a DNA ligation sequencing method using signal combination coding and belongs to the technical field of biology. The method is characterized by connecting signal codes in a ligation sequencing reaction, realizing the sequencing process of synchronously detecting base types in a number which is a power of the number of markers in one ligation reaction by using the same number of markers, exponentially reducing sequencing time, considerably improving sequencing throughput and reducing sequencing cost. The method uses the combinations of the signal statuses in the detection of the prior a plurality of markers for coding, makes the combinations correspond to the types of detected bases one by one and improves the resolution power for markers in the same number due to the fact that the number of the combinations of the signal statuses of the plurality of markers exponentially grows with the increase of the number of the types of the markers, thereby detecting the base types in the number which is the power of the number of the markers in one sequencing reaction.

Provided herein are biologic sample preparation and analysis systems that are rapid, portable, robust detection system for multiplexed detection of bio-threats, and which can be ruggedized to operate in harsh environments. A new method of detection called Combinatorial Probe Analysis (CPA), which provides an exponential increase in detection reliability, has been incorporated into these systems. This type of analysis greatly reduces false positives and false negatives; in addition it is reusable and eliminates special storage requirements for reagents. Specific technical advancements in the optimization of hybridization assays for nucleic acid detection on porous polymer monoliths (PPM) are also disclosed. Performing rapid and complete solubilization of viruses, vegetative bacteria and bacterial spores with an ultra high temperature solubilization protocol is also described. The systems provided herein provides the ability to perform rapid highly multiplexed analysis of a variety of bioagents, including bacteria viruses, and protein biotoxins. The systems and assays described herein are perform completely automated sample preparation and analysis, in a time frame of five minutes or less. The assay is simple in design allowing users in personal protective equipment to easily operate the system. The disclosed systems are robust, simple to use, and address the goals of the first responder community.

The invention belongs to the technical field of medicines, and relates to a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of culturing a seed stock solution in a glass apparatus with a drainage system, and carrying out later amplification culture of the haematococcus pluvialis and accumulation culture of the astaxanthin after 10-15 d; centrifuging a culture in an exponential growth period of seed culture; and inoculating cell clusters in a BBM basal medium to obtain a primary culture. The later accumulation culture of the astaxanthin is amplification culture by using a breathable plastic bag type simple device provided by the invention. In the accumulation stage, a stress culturing method is adopted to obtain a lab-scale test haematococcus pluvialis culture; and haematococcus pluvialis powder is obtained by spray drying. According to a preparation technology that extracts astaxanthin from the haematococcus pluvialis by adopting an ultrasonic cell disruption assisted mixed solvent extraction method, the haematococcus pluvialis powder is added in an organic solvent to carry out ultrasonic cell disruption, and then the astaxanthin is obtained by the steps of reflux extraction in a water bath, suction filtration, filtrate merging and concentration. Compared with a conventional direct extraction method, the method provided by the invention saves extraction time, and increases astaxanthin yield.

The invention provides an adiabatic acceleration calorimetric method based on machine learning. The method comprises the following steps: obtaining a mathematical nonlinear exponential growth fittingformula for accurately describing the adiabatic heat release process through fitting of a mathematical nonlinear modelaccording to actually measured temperature rise data of sample in heat release stage, and enabling the temperature control system of the adiabatic acceleration calorimeter to perform programmed temperature rise according to the obtained fitting formula, and obtaining an experimental curve closer to the actual adiabatic temperature rise process of the sample so that more accurate adiabatic calorimetric measurement of the thermal runaway process is realized. According to the method, measurement errors caused by temperature tracking lag are overcome, and later thermal and dynamic analysis and evaluation can be carried out more fully and accurately; the finally obtained adiabatic thermal experiment curve can effectively improve the accuracy of subsequent thermodynamic calculation of the sample, and has important guiding significance for guiding implementation of reaction safety risk evaluation and chemical safety evaluation.

The invention discloses an airspace operation situation evaluation and classification method based on fuzzy reasoning and belongs to the technical field of airspace situation evaluation and classification. The method comprises the following steps: 1, processing airspace operation situation samples in a to-be-processed sector; 2, establishing a primary fuzzy reasoning system based on the airspace operation situation samples in the to-be-processed sector; 3, optimizing interpretability and accuracy of the fuzzy reasoning system on the basis of a multi-target population adaptive immune algorithm.The method provided by the invention can aim at large-scale and high-dimensionality sector operation data and center on airspace operation situation evaluation accuracy and interpretability, the multi-target immune optimization algorithm is used, and the airspace situation evaluation accuracy is optimized. In addition, when the immune algorithm is realized, the condition that the fuzzy matrix scale is in exponential growth in the process of processing high-dimensionality data is avoided, time complexity and space complexity needed by the algorithm are greatly reduced, and the convergence precision is improved.

The invention belongs to the field of laser three-dimensional imaging, and particularly relates to a laser three-dimensional imaging system based on a human-vision-based simulated mechanism. The system comprises a trigger, a pulse laser, an emitting optimal module, a non-even collection module, a signal processing module and a displayer. By adopting the non-even collection module, after a target is irradiated by the pulse laser, reflected or scattered pulse echoes pass through a first lens, a second lens and a third lens in sequence, and imaging is carried out on a probe array board. The probe array board is composed of a series of independent probes, wherein the number of probes in each ring is the same, the probes are annularly arrayed, each ring of probes and each corresponding adjacent ring of probes are in a tangent relation, adjacent pixels are in a tangent relation, the radiuses of the rings of probes increase exponentially, facula areas of the rings of probes increase exponentially, and accordingly variable-resolution-ratio target collection can be achieved; optical signals are converted to electric signals by a probe array, and the electric signals are read from inner rings to outer rings sequentially by the signal processing module in an annular switch-on mode. Fast and efficient data transmission is achieved.

The invention discloses an adaptive network congestion control method based on SCPS-TP, comprising the following steps: a SCPS-TP gateway source end receives a data packet and forwards the data packet to a destination end; whether there is new data acknowledged is judged according to parsed packet information; if there is no new data acknowledged, the window size growth mode is changed to a linear adaptive mode when a repeated ACK counter increases to a certain value; if there is new data acknowledged, congestion control is in an exponential growth mode, and after the window size is increased, Diff is greater than a set threshold, and the congestion control mode is changed to a linear adaptive mode; if congestion control is in a linear adaptive mode, the window size is adjusted according to Diff; and the SCPS-TP gateway source end sends data in a packet loss buffer region to the destination end, and sends new data according to the size of the congestion window. Network congestion and serious packet loss due to a too long intermediate router queue are avoided, and bandwidth can be fully utilized. A network transmission acceleration function can be achieved when the method is applied to a satellite network with high transmission delay and high link error rate.

The invention discloses a DRX device based on an LTE system and a control method thereof, which belong to the field of mobile communication. The device comprises a service data buffer, a control module, an initial sleep interval calculation module and a sleep interval calculation module, wherein the control module is respectively connected with the service data buffer, the initial sleep interval calculation module and the sleep interval calculation module. The device and the control method thereof realize the variable exponential growth of a sleep period and determine the next initial sleep interval according to the state order of the former sleep mode. A mathematical simulation model is established according to an algorithm, the performance of which is analyzed in the aspects of energy consumption and time delay, and the result of the simulation shows that the improved device and the control method thereof have better performance. The device and the control method thereof has simple structure, low construction cost and stabile and reliable running.

The invention discloses a particle group optimum method of open type car dispatching problem with time window, which is characterized by the following: the calculation quantity of solution course displays exponential increasing along the problem scale, which is testified as NP complete problem; the method adapts the improved cheapest insert algorithm to optimize the car customer sequence, which improves the calculation speed and algorithm precision.

The invention relates to a method for producing tetracycline by fermentation of streptomyces aureus. The method comprises the following steps: slant spore culture, seed culture and fermentation culture of the streptomyces aureus, and is characterized in that dextrin, sucrose, corn pulp, hydrolyzed soybean meal and other medium-effect carbon-nitrogen sources are adopted to replace original late-effect carbon-nitrogen sources; by reducing the contents of starch and bean flour in an original fermentation culture medium and combining with a formula of a mixture in the fermentation process and adjustment of a replenishing method and placing part of the nitrogen sources in the fermentation culture medium into material replenishment in the fermentation process, the concentration of the culture medium in the fermentation process is effectively controlled, the oxygen dissolution condition in the early stage of fermentation is improved, the final residue of the culture medium of fermentation is controlled by material replenishment, the material consumption is reduced, and the emission of solid wastes is reduced; the metabolism velocity in exponential growth phase in the fermentation process of the tetracycline is effectively controlled, the abnormal situations that a fermentation solution turns red and becomes sour and the like in the fermentation process are reduced, and the fermentation level is improved.

The invention discloses a long code DSSS signal blind dispreading method based on semi-definite programming. A long code DSSS signal spread-spectrum waveform maximum likelihood estimation problem with exponential growth calculation complexity is converted to a semi-definite programming problem with polynomial calculation complexity, the semi-definite programming problem is then solved, the approximate solution of the spread-spectrum waveform maximum likelihood estimation problem is acquired, the spread-spectrum waveform estimation is acquired, and accordingly long code DSSS signal blind dispreading is finally finished. Regarding to long code DSSS signals modulated by BPSK under the multi-path channel condition, the long code DSSS signal blind dispreading method greatly reduces calculation complexity of spread-spectrum waveform maximum likelihood estimation through the adoption of semi-definite programming, can still have good performance even under low signal to noise ratio or short data conditions, and is particularly suitable for blind estimation of long code DSSS signals in the non-cooperative communication field.

The invention discloses a digital PCR concentration calculation method, which determines and calculates the initial concentration of a reactant according to the amplification cycleCt value of the real-time fluorescence quantitative curve in the PCR amplification process combined with the real-time clustering method. The fluorescence threshold R11 has an intersection with the amplification curve ofeach positive reaction unit during exponential growth, and the intersection corresponds to the corresponding amplification cycle value Cti. The digital PCR concentration calculation method specifically includes the following steps: clustering according to the Cti, obtaining k clusters, wherein the central value of each cluster is M1, M2, ..., Mk in descending order; calculating that the number ofthe Cti contained in each cluster is Sj, calculating the number of target genes in each cluster by an Mj and the Sj, adding up the number of target genes in each cluster, finally obtaining the concentration value of the initial target genes.

The invention discloses a method for raising hydrogen production efficiency of microalgae and belongs to the field of a biological energy source. The method comprises the following steps: hydrogen-production microalgae cells are cultured in a medium until the exponential growth phase; the microalgae cells are collected, and the microalgae cells are transferred to a microalgae hydrogen-production medium containing organic matters, deoxygenization and sealing are carried out, and the microalgae cells are continuously cultured in a dark environment for a certain time, wherein the organic matters contain at least one of glucose, fructose, acetate, pyruvic acid, 3-phosphoglycerate, malic acid and amino acid and at least one of sodium salts and sylvite of the above organic matters; and finally, the microalgae cells are again placed in a light environment to induce water decomposition to produce hydrogen. It is verified through experiments that hydrogen production efficiency of microalgae can be remarkably raised by adding the organic matters to the microalgae hydrogen-production medium. The method has characteristics of novel technology and remarkable effect and the like, is simple and reliable, and has important practical application value in the field of new energy.