4results about How to "Efficient accumulation" patented technology

This invention discloses an extremely salt-tolerant halophilic bacterium, HD16, which can grow under extreme salinity conditions up to 4.0 M sodium chloride (approximately 230 g / L sodium chloride) and also exhibits high alkalinity tolerance, surviving at pH 7–11. Therefore, it is predicted to have broad application prospects in wastewater treatment and other fields. Furthermore, it can be used to produce polyhydroxyalkali (PHA). When cultured in a 500 ml Erlenmeyer flask, the cell dry weight yield reaches 9.45 g / L, with PHA accounting for 72.97% of the bacterial dry weight. The extreme salt tolerance and high alkalinity tolerance of this strain enable it to efficiently accumulate polyhydroxyalkali esters under open, non-sterile conditions.

The invention discloses a method for increasing the content of gamma-aminobutyric acid in traditional Chinese medicine through fermentation. The method comprises the following steps: S1, superfine grinding of raw materials; s2, performing enzymolysis treatment; s3, fermentation treatment; lactobacillus plantarum and lactobacillus reuteri are selected to be matched for fermentation. A compound enzyme system (cellulase, pectinase and hemicellulase) is added, and the enzyme addition amount is 600 U / g-1400 U / g; the content of GABA in a bacterium synergistic fermentation system is obviously higher than that of a single bacterium fermentation system. Experimental data show that the GABA content can reach 131.5 + / -0.7 [mu] mol / g under the synergistic fermentation condition for 48 h, the GABA content under the lactobacillus plantarum single bacterium fermentation condition and the GABA content under the lactobacillus reuteri single bacterium fermentation condition are 45.5 + / -1.1 [mu] mol / g and 29.9 + / -2.1 [mu] mol / g respectively, the GABA yield is increased by about 2.9-4.4 times through synergistic fermentation, and it is proved that the method can effectively promote efficient conversion and accumulation of glutamic acid into GABA.

This invention provides an activatable semiconductor polymer, its preparation method, and its applications. The activatable semiconductor polymer of this invention can efficiently accumulate at tumor sites. Due to the presence of electron-withdrawing groups on its side chains, the polymer's fluorescence is quenched. Upon response to tumor markers—biothiols, reactive oxygen species, and cathepsin B—the polymer's fluorescence recovers, enabling precise imaging and sonodynamic therapy at the tumor site. Simultaneously, the activatable semiconductor polymer of this invention can label immune cells (such as dendritic cells and macrophages) and track their metastasis from the tumor to the draining lymph nodes.

This invention provides an L-homoserine-producing strain, its construction method, and its applications. The strain does not contain plasmids. E.coli W3110, as a chassis strain, had the relA and thrB genes knocked out, and heterologously expressed genes derived from... Clostridium acetobutylicum The gapC gene and the aspB gene derived from Bacillus subtilis were overexpressed, along with ppc, asd, and thrA. fbr ,rhtA,pntAB,spoT fbr Genes; the strain described above can effectively improve the L-homoserine synthesis efficiency by knocking out genes of the L-homoserine degradation pathway, enhancing the expression of key enzymes in the pathway, optimizing the L-homoserine transport system, increasing the reducing power NADPH content, and enhancing the ability to withstand amino acid starvation. It is genetically stable, requires no addition of resistance substances, has high acid production efficiency, and has good L-homoserine synthesis capacity.