24results about How to "Efficient accumulation" patented technology

The invention discloses a strain of desert algae improved by space breeding and application of the desert algae. According to the invention, a strain of desert algae is obtained through variation induced by a space environment and ground screening, and the strain of desert algae shows high growth rate and excellent outdoor adaptability, and can efficiently accumulate two bioactive substances, namely algal polysaccharides and flavones, which have important commercial and health values. Therefore, the strain of the desert algae shows better application potential in the aspects of producing high-quality desert algae powder, polysaccharide, flavone and the like.

The invention relates to a method for realizing rapid nitrous accumulation through short-cut nitrification and endogenous short-cut denitrification coupled flora, and belongs to the technical field of nitrogen-containing sewage treatment in environmental protection. According to the method disclosed by the invention, short-cut nitrification and endogenous short-cut denitrification coupling is realized, rapid nitrous accumulation can be realized, an organic carbon source does not need to be consumed, and the consumption of substances and energy is reduced. Meanwhile, the method provided by the invention not only can be used for treating the wastewater containing ammonia nitrogen, but also can be used for treating the wastewater containing nitrate nitrogen, solves the problem that nitrate nitrogen is generated due to partial whole-process nitrification in short-cut nitrification, and can ensure high-efficiency and long-term stable accumulation of nitrous; and a quick starting method is provided for a sewage plant adopting a short-cut denitrification related process.

According to the invention, by optimizing the expression of related genes in the production path of recombinant microorganism 5-hydroxytryptophan and screening and strengthening the gene expression of 5-hydroxytryptophan to extracellular transport protein, the toxicity of product accumulation on thallus growth is greatly reduced. According to the present invention, the efficient synthesis of 5-hydroxytryptophan using glucose or glycerol as the carbon source is achieved, the 5-hydroxytryptophan is co-produced under the action of tryptophan hydroxylase by adding a part of tryptophan in the fermentation system as the substrate, and the conversion from the low-value carbon source to the high-added-value product is achieved.

The invention discloses a method for continuously preparing nano-emulsion in an ultrasonic micro-packed bed reactor. According to the ultrasonic micro-packed bed reactor disclosed by the invention, the large-radiating-surface horn-shaped sandwich type transducer and the capillary microreactor are glued, and the solid filler is assembled in the microreactor, so that effective interception of acoustic cavitation bubbles under the condition of high liquid phase flow can be realized, and the ultrasonic energy efficiency is improved. The capillary microreactors are connected through a stepped structure, so that the filler can be freely detached, and the structure, size and material of the filler are convenient to replace. Through continuous conveying equipment, a continuous phase and a dispersed phase are mixed and emulsified in the reactor, and efficient and rapid preparation (2-180s) of the nano-emulsion (the average size is 20-500nm) can be realized. According to the method, the preparation time of the nano-emulsion is shortened (5-60 minutes in the prior art), continuous production is realized, and the method is simple and convenient to operate and easy to integrate and amplify in parallel.

The invention discloses a genetic engineering strain of high-yield secondary metabolite Fumagillin as well as a construction method and application of the genetic engineering strain, and the genetic engineering strain is obtained by taking aspergillus fumigatus as a chassis bacterium through hosA gene knockout or hosA catalytic active site mutation. According to the method, a hosA gene knockout strain (delta hosA) and catalytic active site mutation strains (HosAD133A, HosAH175A and HosAD210A) are constructed by means of molecular biology, and the mutant strains are subjected to liquid or solid fermentation under the culture condition of 37 DEG C, so that the yield of Fumagillin can be remarkably increased. According to the method disclosed by the invention, the inhibition on a Fumagillin biosynthetic pathway is relieved and the metabolic flux is enhanced by utilizing the activity change of histone deacetylase caused by hosA defects, and an efficient and directional production method for high-yield Fumagillin is provided, so that the method has an important industrial application value.

This invention discloses an extremely salt-tolerant halophilic bacterium, HD16, which can grow under extreme salinity conditions up to 4.0 M sodium chloride (approximately 230 g / L sodium chloride) and also exhibits high alkalinity tolerance, surviving at pH 7–11. Therefore, it is predicted to have broad application prospects in wastewater treatment and other fields. Furthermore, it can be used to produce polyhydroxyalkali (PHA). When cultured in a 500 ml Erlenmeyer flask, the cell dry weight yield reaches 9.45 g / L, with PHA accounting for 72.97% of the bacterial dry weight. The extreme salt tolerance and high alkalinity tolerance of this strain enable it to efficiently accumulate polyhydroxyalkali esters under open, non-sterile conditions.

The invention relates to the technical field of microbial fermentation, in particular to a nucleoside production strain as well as a construction method and application thereof, and particularly relates to a recombinant microorganism with DNA mismatch repair protein mutation and application thereof in nucleoside production. By strengthening the DNA mismatch repair protein or the coding gene thereof, the nucleoside production capacity and production efficiency of microorganisms are remarkably improved, and a new modification target and strategy are provided for construction of nucleoside production strains. The DNA mismatch repair protein variant provided by the invention can effectively promote microorganisms to accumulate nucleoside and remarkably improve the nucleoside production capacity of the microorganisms. According to the method for improving the nucleoside production capacity and the recombinant microorganism constructed by the DNA mismatch repair protein variant provided by the invention, nucleoside can be more efficiently accumulated, and the nucleoside yield is remarkably improved.

The invention relates to the technical field of intellectual property data processing, and discloses an intellectual property data collection method and system based on multi-source data fusion, and the method comprises the steps: obtaining an original information set, and carrying out the clustering and grouping processing of the original information set, and obtaining a classification data set; performing feature verification and matching analysis on the classification data set to obtain a feature matching score; if the feature matching score is higher than a preset analysis threshold value, performing structured analysis on the classification data set to obtain a preliminary analysis field; performing standardized mapping processing on the preliminary analysis field to obtain data in a unified format; performing associated value mining on the data in the unified format to obtain high-value associated information; and fusing the high-value associated information to a preset overall data set to obtain a target intellectual property data set. According to the method, efficient integration and deep value mining of multi-source heterogeneous data can be realized, and the acquisition precision and standardization level of intellectual property data are remarkably improved.

The invention relates to a synthesis method and application of a small molecule compound (IR-TAM) targeting tumor cell mitochondria. The method comprises the following steps: synthesizing IR-68-NHS by using IR-68, and carrying out a reaction on a certain amount of IR-68-NHS and TAM-NH to obtain IR-TAM; the small molecule compound IR-TAM with tumor mitochondrial targeting is designed through a rapid, economical and efficient synthetic route, tumor hypoxia can be effectively reversed through selective mitochondrial metabolism reprogramming, the expression of PD-L1 is regulated and controlled to reverse the tumor microenvironment of immunosuppression, meanwhile, radiotherapy and immunotherapy are enhanced, and the compound IR-TAM can be used for treating solid tumors, tumors, tumors and the like. Wide application prospects are realized in the treatment of metastatic tumors and hematoma. Meanwhile, IR-TAM can also be used for preventing or treating fibrosis diseases induced by radiotherapy.

The invention discloses an emergency evacuation planning method based on improved ant colony algorithm and genetic fusion, and belongs to the technical field of emergency management and public safety. The method comprises the following steps: constructing a multi-objective optimization model taking minimization of total evacuation time T, total road section risk R and total personnel panic index P as objectives, and solving the multi-objective optimization model based on fusion of an improved ant colony algorithm and a genetic algorithm to obtain an optimal evacuation path scheme. The evacuation efficiency, safety and personnel psychological factors are comprehensively considered, the state transition probability and the pheromone updating strategy of the ant colony algorithm are improved, the global search mechanism of the genetic algorithm is introduced for deep fusion, and finally, the evacuation efficiency can be improved in a complex evacuation scene. And a global optimal evacuation path giving consideration to timeliness, safety and personnel psychological factors is quickly planned, and the evacuation efficiency is effectively improved.

The invention belongs to the field of plant genetic engineering, and provides a nucleotide molecule, a recombinant vector and a recombinant host cell for silencing a squalene epoxidase gene NtSQE1 in tobacco and application of the nucleotide molecule, the recombinant vector and the recombinant host cell in increasing the squalene content in the tobacco, and the SEQ ID NO: 2 sequence in the squalene epoxidase gene NtSQE1 is inhibited in a targeted mode through a specific virus-induced gene silencing technology (VIGS). The accumulation level of squalene in a plant body can be remarkably improved under the condition that the plant phenotype is not influenced, and the silencing efficiency is high.

This invention provides an activatable semiconductor polymer, its preparation method, and its applications. The activatable semiconductor polymer of this invention can efficiently accumulate at tumor sites. Due to the presence of electron-withdrawing groups on its side chains, the polymer's fluorescence is quenched. Upon response to tumor markers—biothiols, reactive oxygen species, and cathepsin B—the polymer's fluorescence recovers, enabling precise imaging and sonodynamic therapy at the tumor site. Simultaneously, the activatable semiconductor polymer of this invention can label immune cells (such as dendritic cells and macrophages) and track their metastasis from the tumor to the draining lymph nodes.

The invention is applicable to the technical field of biorefinery of oleaginous yeast, and provides a method for producing grease by efficiently utilizing starch-rich plant-derived traditional Chinese medicine residues, which comprises the following steps: sterilizing selected plant-derived wet traditional Chinese medicine residues, preparing into a culture medium taking the traditional Chinese medicine residues as a unique component, and inoculating lipomyces starkeyi for fermentation without or with an extremely small amount of cellulase; in the fermentation process, amylase produced by yeast and additional cellulase jointly act on the traditional Chinese medicine residues, and the traditional Chinese medicine residues are rapidly degraded into fermentable sugar which is efficiently used for rapid production of grease. According to the method, the traditional Chinese medicine residues do not need to be subjected to any pretreatment, amylase production, enzyme hydrolysis and fermentation are highly integrated, the number of equipment is greatly reduced, the technological process is simplified, the production cost of the microbial oil is remarkably reduced, and a novel low-cost method is provided for solving the problems of treatment and recycling of large-block traditional Chinese medicine residues. The fatty acid composition of the produced microbial oil is similar to that of vegetable oil, and the microbial oil can be used as a substitute raw material of biodiesel.

A first step is preparing a sample in contact with a bowl region. A second step is irradiating the bowl region with a laser beam to generate a microbubble in an irradiation region of the laser beam and accumulate a plurality of microscopic objects around the microbubble. The bowl region includes a metallic thin film (211), a conductive polymer film (112), and a metallic thin film (113). Metallic thin film (211) includes a material that converts the laser beam into heat. Conductive polymer film (112) has a plurality of bowl-like structures periodically arranged on metallic thin film (211). Metallic thin film (113) includes a material that converts the laser beam into heat and is disposed on at least a part of conductive polymer film (112). The sizes of the plurality of bowl-like structures and the irradiation region of the laser beam are determined such that at least two of the plurality of bowl-like structures are entirely included in the irradiation region.

The invention discloses a composite micelle co-loaded with an immune checkpoint blocking agent as well as a preparation method and application of the composite micelle. The preparation method comprises the following steps: mixing PCL3K-TK-NHS, CVRARTR and a solvent, stirring, dialyzing, and drying to obtain PCL3K-TK-CVRARTR; the preparation method comprises the following steps: carrying out amidation on PCL3K-NH2 and NHS-PEG5K-RGD, so as to obtain PCL3K-PEG5K-RGD; the preparation method comprises the following steps: mixing PCL3k-TK-CVRARTR, PCL3K-PEG5K-RGD, 1-MT and a solvent, adding the mixture into PBS (Phosphate Buffer Solution) to form a micelle, dialyzing, and removing the solvent to obtain the composite micelle. The preparation method disclosed by the invention is simple and has the advantages of low cost, low toxicity and high efficiency, and the prepared composite micelle can simultaneously transport the IDO blocking agent 1-MT and the antagonistic PD-L1 polypeptide to a tumor site, so that effective accumulation of the IDO blocking agent 1-MT and the antagonistic PD-L1 polypeptide at the tumor site is realized, an immune checkpoint is cooperatively blocked, and the anti-tumor effect of an immune system is recovered.

The application provides a forming process for an inner diameter deep cavity ring type forging, which comprises the following steps: ring rolling preforming, die forging preforming, ring rolling preforming, ring rolling final forging forming and the like. Through the above method, effective accumulation of metal materials in the inner diameter deep cavity is realized, and the flash of the forging and the forming defects of the forging are reduced.

The utility model relates to the technical field of Chinese chive harvesting equipment, and provides small Chinese chive harvesting equipment with precise cutting and automatic bundling, the equipment consists of an upper rack and a lower rack, and the rotation of the upper rack relative to the lower rack is realized through a slewing mechanism. The upper rack is provided with a righting mechanism, a conveying mechanism and a bundling mechanism, and the lower rack is provided with a cutting mechanism and a steering mechanism. The righting mechanism gathers the Chinese chives through a grain lifter of a cambered surface structure, the conveying mechanism stably transits the Chinese chives through a sponge synchronous belt, and the bundling mechanism achieves automatic bundling through a supporting claw mechanism and a rubber band clamping claw. And the cutting mechanism adopts a blade lifting mechanism and a blade driving mechanism to realize accurate cutting. The steering mechanism adopts the Ackerman steering principle, and steering efficiency and stability are improved. The equipment is high in adaptability, harvesting efficiency and automation degree, the labor cost is effectively reduced, and the agricultural production efficiency is improved.

The invention belongs to the technical field of biology, and particularly relates to trans-aconitic acid transporter and application thereof. The trans-aconitic acid (TAA) transporter provided by the invention has an effect of transporting extracellular TAA into microbial cells, plays an important role in TAA metabolic pathway utilization by microorganisms, provides a new genetic resource for regulation and control of synthesis and utilization of microbial TAA, has a good application prospect in the fields of screening, cultivation and improvement of TAA efficient utilization bacteria, and has a wide application prospect. And a new method and thought are provided for enhancing the carbon assimilation capability of the probiotics, improving the rhizosphere colonization capability and the like. Meanwhile, by expressing the protein in plants, microorganisms and the like, the nematicidal activity of the protein is improved, and a new thought is provided for biological control of nematode infection. In addition, the TAA transporter provided by the invention can be specifically combined with TAA molecules with high affinity, and a specific carrier material and a feasible way are provided for affinity chromatography purification of TAA.

The invention discloses a method for increasing the content of gamma-aminobutyric acid in traditional Chinese medicine through fermentation. The method comprises the following steps: S1, superfine grinding of raw materials; s2, performing enzymolysis treatment; s3, fermentation treatment; lactobacillus plantarum and lactobacillus reuteri are selected to be matched for fermentation. A compound enzyme system (cellulase, pectinase and hemicellulase) is added, and the enzyme addition amount is 600 U / g-1400 U / g; the content of GABA in a bacterium synergistic fermentation system is obviously higher than that of a single bacterium fermentation system. Experimental data show that the GABA content can reach 131.5 + / -0.7 [mu] mol / g under the synergistic fermentation condition for 48 h, the GABA content under the lactobacillus plantarum single bacterium fermentation condition and the GABA content under the lactobacillus reuteri single bacterium fermentation condition are 45.5 + / -1.1 [mu] mol / g and 29.9 + / -2.1 [mu] mol / g respectively, the GABA yield is increased by about 2.9-4.4 times through synergistic fermentation, and it is proved that the method can effectively promote efficient conversion and accumulation of glutamic acid into GABA.

This invention provides an L-homoserine-producing strain, its construction method, and its applications. The strain does not contain plasmids. E.coli W3110, as a chassis strain, had the relA and thrB genes knocked out, and heterologously expressed genes derived from... Clostridium acetobutylicum The gapC gene and the aspB gene derived from Bacillus subtilis were overexpressed, along with ppc, asd, and thrA. fbr ,rhtA,pntAB,spoT fbr Genes; the strain described above can effectively improve the L-homoserine synthesis efficiency by knocking out genes of the L-homoserine degradation pathway, enhancing the expression of key enzymes in the pathway, optimizing the L-homoserine transport system, increasing the reducing power NADPH content, and enhancing the ability to withstand amino acid starvation. It is genetically stable, requires no addition of resistance substances, has high acid production efficiency, and has good L-homoserine synthesis capacity.

The invention belongs to the technical field of radar signal processing, and particularly relates to a carrier frequency and repetition frequency random agility semi-active pulse radar phase-coherent accumulation method. The method specifically comprises the following steps: carrying out carrier frequency identification on a reference pulse signal received by a semi-active pulse radar receiver, carrying out digital orthogonal down-conversion processing on a residual intermediate frequency component identified in the carrier frequency identification process to obtain a zero intermediate frequency reference pulse signal, and carrying out repetition frequency identification on the zero intermediate frequency reference pulse signal; processing the radio frequency signal within the echo pulse signal receiving time to obtain a zero intermediate frequency echo pulse signal; using the zero intermediate frequency reference pulse signal to perform correlation matching processing on the receiving component of the zero intermediate frequency echo pulse signal to select a target signal; on the basis of a repetition frequency identification result, performing signal phase-coherent accumulation on the target signal by adopting non-uniform Fourier transform; and searching the range gate, the speed and the acceleration.

The invention belongs to the field of plant genetic engineering, and provides a nucleotide molecule, a recombinant vector and a recombinant host cell for silencing a squalene epoxidase gene NtSQU2 of tobacco and application of the nucleotide molecule, the recombinant vector and the recombinant host cell in increasing the squalene content in the tobacco, and the sequence SEQ ID NO: 3 in the squalene epoxidase gene NtSQU2 is inhibited in a targeted mode through a specific virus-induced gene silencing technology (VIGS). The accumulation level of squalene in a plant body can be remarkably improved under the condition that the plant phenotype is not influenced, and the silencing efficiency is high.

The application relates to a UTG-based solar cell substrate and a solar cell. The UTG-based solar cell substrate comprises a connecting layer, a first UTG substrate layer, a growth substrate layer and an epitaxial growth layer. The connecting layer is arranged on one side of the first UTG substrate layer. The growth substrate layer is arranged on the side, away from the connecting layer, of the first UTG substrate layer. The growth substrate layer comprises at least one of a copper layer, an iridium layer, a hexagonal boron nitride layer and a silicon carbide layer, and the epitaxial growth layer comprises a graphene layer arranged on the side, away from the first UTG substrate layer, of the growth substrate layer. The UTG substrate layer has excellent characteristics such as high light transmittance, high barrier property, atomic oxygen resistance, radiation resistance and bendability. The growth substrate layer has good heat conduction performance. The graphene layer can be epitaxially grown on the growth substrate layer easily, and the graphene layer has high infrared emission performance and high conductivity, can realize passive and efficient heat dissipation, avoid accumulation of static electricity, and prevent the internal circuit of the battery from being broken.

The application discloses a dry land spring maize planting method and application, and relates to the field of agricultural technology. The method comprises the following steps: rotary tillage after spreading fertilizer on a planting site; land preparation before sowing and setting wide rows and narrow rows, and covering the wide rows with mulching film; planting on the wide rows after covering the mulching film; and laying soil moisture sensors. The fertilizer comprises a high-molecular water-retaining agent and a nutrient base. The dry-land water-saving and synergistic fertilizer is organically combined with the ridge and furrow rain collection and mulching technology. The ridge and furrow rain collection efficiently collects rainfall in the crop root zone, and the mulching film significantly reduces evaporation loss. The dry-land water-saving and synergistic fertilizer further absorbs and retains water, forming a complete water management system of "water collection, water retention and water supply", so that efficient collection and utilization of natural rainfall are realized, water and fertilizer supply are strengthened, and the planting density under limited water conditions is improved.