12results about How to "Efficient accumulation" patented technology

The invention discloses a genetic engineering strain of high-yield secondary metabolite Fumagillin as well as a construction method and application of the genetic engineering strain, and the genetic engineering strain is obtained by taking aspergillus fumigatus as a chassis bacterium through hosA gene knockout or hosA catalytic active site mutation. According to the method, a hosA gene knockout strain (delta hosA) and catalytic active site mutation strains (HosAD133A, HosAH175A and HosAD210A) are constructed by means of molecular biology, and the mutant strains are subjected to liquid or solid fermentation under the culture condition of 37 DEG C, so that the yield of Fumagillin can be remarkably increased. According to the method disclosed by the invention, the inhibition on a Fumagillin biosynthetic pathway is relieved and the metabolic flux is enhanced by utilizing the activity change of histone deacetylase caused by hosA defects, and an efficient and directional production method for high-yield Fumagillin is provided, so that the method has an important industrial application value.

This invention discloses an extremely salt-tolerant halophilic bacterium, HD16, which can grow under extreme salinity conditions up to 4.0 M sodium chloride (approximately 230 g / L sodium chloride) and also exhibits high alkalinity tolerance, surviving at pH 7–11. Therefore, it is predicted to have broad application prospects in wastewater treatment and other fields. Furthermore, it can be used to produce polyhydroxyalkali (PHA). When cultured in a 500 ml Erlenmeyer flask, the cell dry weight yield reaches 9.45 g / L, with PHA accounting for 72.97% of the bacterial dry weight. The extreme salt tolerance and high alkalinity tolerance of this strain enable it to efficiently accumulate polyhydroxyalkali esters under open, non-sterile conditions.

The invention relates to the technical field of intellectual property data processing, and discloses an intellectual property data collection method and system based on multi-source data fusion, and the method comprises the steps: obtaining an original information set, and carrying out the clustering and grouping processing of the original information set, and obtaining a classification data set; performing feature verification and matching analysis on the classification data set to obtain a feature matching score; if the feature matching score is higher than a preset analysis threshold value, performing structured analysis on the classification data set to obtain a preliminary analysis field; performing standardized mapping processing on the preliminary analysis field to obtain data in a unified format; performing associated value mining on the data in the unified format to obtain high-value associated information; and fusing the high-value associated information to a preset overall data set to obtain a target intellectual property data set. According to the method, efficient integration and deep value mining of multi-source heterogeneous data can be realized, and the acquisition precision and standardization level of intellectual property data are remarkably improved.

The invention relates to a synthesis method and application of a small molecule compound (IR-TAM) targeting tumor cell mitochondria. The method comprises the following steps: synthesizing IR-68-NHS by using IR-68, and carrying out a reaction on a certain amount of IR-68-NHS and TAM-NH to obtain IR-TAM; the small molecule compound IR-TAM with tumor mitochondrial targeting is designed through a rapid, economical and efficient synthetic route, tumor hypoxia can be effectively reversed through selective mitochondrial metabolism reprogramming, the expression of PD-L1 is regulated and controlled to reverse the tumor microenvironment of immunosuppression, meanwhile, radiotherapy and immunotherapy are enhanced, and the compound IR-TAM can be used for treating solid tumors, tumors, tumors and the like. Wide application prospects are realized in the treatment of metastatic tumors and hematoma. Meanwhile, IR-TAM can also be used for preventing or treating fibrosis diseases induced by radiotherapy.

The invention discloses an emergency evacuation planning method based on improved ant colony algorithm and genetic fusion, and belongs to the technical field of emergency management and public safety. The method comprises the following steps: constructing a multi-objective optimization model taking minimization of total evacuation time T, total road section risk R and total personnel panic index P as objectives, and solving the multi-objective optimization model based on fusion of an improved ant colony algorithm and a genetic algorithm to obtain an optimal evacuation path scheme. The evacuation efficiency, safety and personnel psychological factors are comprehensively considered, the state transition probability and the pheromone updating strategy of the ant colony algorithm are improved, the global search mechanism of the genetic algorithm is introduced for deep fusion, and finally, the evacuation efficiency can be improved in a complex evacuation scene. And a global optimal evacuation path giving consideration to timeliness, safety and personnel psychological factors is quickly planned, and the evacuation efficiency is effectively improved.

The invention belongs to the field of plant genetic engineering, and provides a nucleotide molecule, a recombinant vector and a recombinant host cell for silencing a squalene epoxidase gene NtSQE1 in tobacco and application of the nucleotide molecule, the recombinant vector and the recombinant host cell in increasing the squalene content in the tobacco, and the SEQ ID NO: 2 sequence in the squalene epoxidase gene NtSQE1 is inhibited in a targeted mode through a specific virus-induced gene silencing technology (VIGS). The accumulation level of squalene in a plant body can be remarkably improved under the condition that the plant phenotype is not influenced, and the silencing efficiency is high.

This invention provides an activatable semiconductor polymer, its preparation method, and its applications. The activatable semiconductor polymer of this invention can efficiently accumulate at tumor sites. Due to the presence of electron-withdrawing groups on its side chains, the polymer's fluorescence is quenched. Upon response to tumor markers—biothiols, reactive oxygen species, and cathepsin B—the polymer's fluorescence recovers, enabling precise imaging and sonodynamic therapy at the tumor site. Simultaneously, the activatable semiconductor polymer of this invention can label immune cells (such as dendritic cells and macrophages) and track their metastasis from the tumor to the draining lymph nodes.

The application provides a forming process for an inner diameter deep cavity ring type forging, which comprises the following steps: ring rolling preforming, die forging preforming, ring rolling preforming, ring rolling final forging forming and the like. Through the above method, effective accumulation of metal materials in the inner diameter deep cavity is realized, and the flash of the forging and the forming defects of the forging are reduced.

The invention discloses a method for increasing the content of gamma-aminobutyric acid in traditional Chinese medicine through fermentation. The method comprises the following steps: S1, superfine grinding of raw materials; s2, performing enzymolysis treatment; s3, fermentation treatment; lactobacillus plantarum and lactobacillus reuteri are selected to be matched for fermentation. A compound enzyme system (cellulase, pectinase and hemicellulase) is added, and the enzyme addition amount is 600 U / g-1400 U / g; the content of GABA in a bacterium synergistic fermentation system is obviously higher than that of a single bacterium fermentation system. Experimental data show that the GABA content can reach 131.5 + / -0.7 [mu] mol / g under the synergistic fermentation condition for 48 h, the GABA content under the lactobacillus plantarum single bacterium fermentation condition and the GABA content under the lactobacillus reuteri single bacterium fermentation condition are 45.5 + / -1.1 [mu] mol / g and 29.9 + / -2.1 [mu] mol / g respectively, the GABA yield is increased by about 2.9-4.4 times through synergistic fermentation, and it is proved that the method can effectively promote efficient conversion and accumulation of glutamic acid into GABA.

This invention provides an L-homoserine-producing strain, its construction method, and its applications. The strain does not contain plasmids. E.coli W3110, as a chassis strain, had the relA and thrB genes knocked out, and heterologously expressed genes derived from... Clostridium acetobutylicum The gapC gene and the aspB gene derived from Bacillus subtilis were overexpressed, along with ppc, asd, and thrA. fbr ,rhtA,pntAB,spoT fbr Genes; the strain described above can effectively improve the L-homoserine synthesis efficiency by knocking out genes of the L-homoserine degradation pathway, enhancing the expression of key enzymes in the pathway, optimizing the L-homoserine transport system, increasing the reducing power NADPH content, and enhancing the ability to withstand amino acid starvation. It is genetically stable, requires no addition of resistance substances, has high acid production efficiency, and has good L-homoserine synthesis capacity.

The invention belongs to the field of plant genetic engineering, and provides a nucleotide molecule, a recombinant vector and a recombinant host cell for silencing a squalene epoxidase gene NtSQU2 of tobacco and application of the nucleotide molecule, the recombinant vector and the recombinant host cell in increasing the squalene content in the tobacco, and the sequence SEQ ID NO: 3 in the squalene epoxidase gene NtSQU2 is inhibited in a targeted mode through a specific virus-induced gene silencing technology (VIGS). The accumulation level of squalene in a plant body can be remarkably improved under the condition that the plant phenotype is not influenced, and the silencing efficiency is high.

The application discloses a dry land spring maize planting method and application, and relates to the field of agricultural technology. The method comprises the following steps: rotary tillage after spreading fertilizer on a planting site; land preparation before sowing and setting wide rows and narrow rows, and covering the wide rows with mulching film; planting on the wide rows after covering the mulching film; and laying soil moisture sensors. The fertilizer comprises a high-molecular water-retaining agent and a nutrient base. The dry-land water-saving and synergistic fertilizer is organically combined with the ridge and furrow rain collection and mulching technology. The ridge and furrow rain collection efficiently collects rainfall in the crop root zone, and the mulching film significantly reduces evaporation loss. The dry-land water-saving and synergistic fertilizer further absorbs and retains water, forming a complete water management system of "water collection, water retention and water supply", so that efficient collection and utilization of natural rainfall are realized, water and fertilizer supply are strengthened, and the planting density under limited water conditions is improved.