226 results about "Homoserine" patented technology

The invention relates to the field of gene engineering, and discloses a D-3-phosphoglycerate dehydrogenase mutant, a coding gene thereof and application of the D-3-phosphoglycerate dehydrogenase mutant and the coding gene. Compared with a protein with an amino acid sequence as shown in SEQ ID NO: 1, the mutant comprises mutation at the 131 site and / or the 285 site. The mutant can specifically catalyze a substrate 3-phosphoglycerate to synthesize 3-phosphate-hydroxypyruvic acid, can effectively promote synthesis of L-serine in a metabolic pathway of microorganisms, improves the yield and conversion rate of the L-serine, and reduces the yield of byproducts at the same time.

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention provides a strain of Ralstonia sp. Regulated by quorum sensing and application thereof, the strain is Ralstonia sp., is named as YTR-R242, has been preserved in China General Microbiological Culture Collection Center (CGMCC) on October 21, 2024, and has a preservation number of CGMCC32269. The strain can generate N-acyl-homoserine lactone, the structure of the strain is C4-HSL, a phosphate-solubilizing ring proves that the Ralstonia sp. Strain YTR-R242 has a phosphate-solubilizing characteristic, and when the Ralstonia sp. Strain YTR-R242 is prepared into a bacterial agent for use, the content of available phosphorus in red soil is remarkably increased, and the growth condition of corn is improved.

The invention relates to the technical field of microbial metabolism engineering, in particular to a construction method and application of genetically engineered bacteria for efficiently producing O-acetyl-L-homoserine. The construction method specifically comprises the following steps: (1) taking a strain Escherichia coli W3110 as a chassis, and knocking out a homoserine O-succinyl transferase coding gene metA; (2) knocking out a gamma-cystathionine synthetase encoding gene metB; (3) knocking out a cysteine lyase coding gene metC; (4) carrying out weakening expression on an O-acetyl-L-homoserine thiolase coding gene metYcm from the marine cyclobacterium; (5) overexpressing a ribulose diphosphate carboxylase coding gene rbcLSse from the synechococcus elongate; (6) overexpressing a ribulose phosphate kinase coding gene prkse from the synechococcus slender; and (7) weakening the expression of the citrate synthase coding gene gltA to obtain the genetically engineered bacterium for efficiently producing the O-acetyl-L-homoserine. The strain provided by the invention is used for producing O-acetyl-L-homoserine, and has the advantages of high yield, high yield and high sugar-acid conversion rate.

The invention discloses a method for liquefying kitchen waste based on quorum sensing enhanced compound bacteria, and relates to the technical field of kitchen waste treatment. According to the invention, quorum sensing molecule N-myristoyl-L-homoserine lactone which plays a role in the composite bacteria liquid is externally added, so that the capability of the composite bacteria liquid for synergistically degrading protein, cellulose, starch and grease in the kitchen waste is obviously improved, the degradation rates are respectively increased from 19%, 38%, 87% and 45% to 30%, 56%, 97% and 54%, and the effect is obvious. According to the method, the liquefaction effect of the kitchen waste is remarkably improved, and the method has a good practical application prospect.

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The invention relates to the technical field of microbial metabolism engineering, in particular to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. The invention relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine, which is characterized in that a transport protein encoding gene rhtA is knocked out in a chassis strain Escherichia coli W3110, a homoserine O-acetyltransferase encoding gene metXcm from marine cyclobacter is over-expressed, an acetyl coenzyme A synthetase encoding gene acs is over-expressed, pantothenic acid kinase coXpp from pseudomonas putida is over-expressed, and the recombinant escherichia coli can be used for efficiently producing O-acetyl-L-homoserine. And knocking out the malate synthetase coding gene aceB to obtain the recombinant escherichia coli for efficiently producing the O-acetyl-L-homoserine. The strain provided by the invention does not carry plasmids, does not need to additionally add antibiotics and inducers, and is lower in production cost and more stable. And the yield of the O-acetyl-L-homoserine of the plasmid-free strain is increased to 16.85 g / L from 0 g / L. Therefore, the recombinant escherichia coli provided by the invention has important industrial application value.

The invention discloses a tyrosine decarboxylase mutant and an application of the tyrosine decarboxylase mutant in fermentation synthesis of 3-amino-1-propanol. The invention relates to the technical field of biology, and provides a tyrosine decarboxylase mutant which is a protein obtained by replacing the 126 amino acid residue of tyrosine decarboxylase with M from S and replacing the 100 amino acid residue with A or V from N. According to the invention, the tyrosine decarboxylase mutant capable of catalyzing an L-homoserine substrate is obtained, and direct synthesis of 3-amino-1-propanol by a biological fermentation method is realized.

The invention discloses a recombinant bacterium for fermentation production of D-panthenol and application of the recombinant bacterium. The invention relates to the technical field of biology, and provides a recombinant bacterium for fermentation production of D-panthenol, which is obtained by expressing tyrosine decarboxylase and D-pantothenic acid synthetase in a chassis host bacterium, the chassis host bacteria are escherichia coli capable of synthesizing D-pantoic acid and L-homoserine by using glucose. Compared with an existing chemical synthesis method, pure biosynthesis from glucose to D-panthenol is achieved, the method has the advantages of being environmentally friendly, low in cost and the like, and a good foundation is laid for large-scale industrial production of D-panthenol.

The invention discloses a soothing composition for relieving itching of a body and application of the soothing composition, and relates to the technical field of cosmetics. The invention discloses a soothing and itching relieving composition, which is prepared from the following ingredients in parts by weight: 0.1 to 0.5 part of phoenix dactylifera seeds, 1 to 5 parts of hyperforin perforatum flower extracts, 1 to 10 parts of Brazil nectarine fruit oil and 0.01 to 0.2 part of L-homoserine. Through cooperation of the phoenix dactylifera seeds, the hyperforin perforatum flower extract, the Brazilian nectarine fruit oil and the L-homoserine, on one hand, when the temperature changes and nursing is improper, sensitive skin can be helped to be calm and relaxed, and the resistance of the skin to external environment influence is enhanced; on the other hand, the composition can synergistically moisturize, relieve and reduce skin sensitivity and prevent skin itch and dryness.

The invention belongs to the technical field of synthetic biology, and particularly relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. According to the invention, CRISPR-Cas9 gene editing is combined with metabolic modification, so as to precisely integrate OAH biosynthesis key nodes: introducing and overexpressing a mutant metX gene at a genome pseudogene site to enhance the expression of homoserine acetyltransferase, knocking out byproducts such as poxB and mgsA to synthesize genes, and carrying out high-yield synthesis of OAH. Corynebacterium glutamicum thrA (anti-feedback inhibition), aspB and bacillus subtilis acsA-acuA genes (construction of an acetic acid switch) are introduced, gene expression of gltA, tpiA and the like is optimized to increase supply of precursors such as acetyl CoA and the like, and finally a stable strain without plasmids, antibiotics and inducers is obtained. The production cost is effectively reduced, and the method has the advantage of high genetic stability and shows a good application prospect in OAH industrial production.

The invention discloses a phycomycete symbiotic system and application of the phycomycete symbiotic system in treatment of aquaculture tail water, and belongs to the technical field of tail water treatment. The phycomycete symbiotic system comprises chlorella, scenedesmus and activated sludge, and the initial inoculation dry weight ratio of the chlorella to the scenedesmus to the activated sludge is 1: 1: 6. And exogenously adding octanoyl-L-homoserine lactone or tetradecanoyl-L-homoserine lactone into the phycomycetes symbiotic system, so as to construct the phycomycetes symbiotic system with enhanced functions. On the basis of a mixed algae-bacteria symbiotic system with an optimized ratio, directional enhancement of functions is realized by virtue of quorum sensing signal molecules, the stability and resistance gene interception capability of the quorum sensing signal molecules under the stress of antibiotics are verified, and finally efficient purification of the aquaculture tail water is realized. According to the invention, the blank of the prior art in the aspects of advanced regulation and control of a phycomycete synergistic mechanism and synergistic treatment of emerging pollutants is filled up.

The present invention provides a method for improving the ability of Escherichia coli to produce O-succinyl-L-homoserine by using adaptive evolution. The method uses Escherichia coli lacking cystathionine-γ-synthase and homoserine kinase and relieving feedback repression as a starting strain, and uses a culture medium containing different O-succinyl-L-homoserine concentrations to perform test tube passage pressure screening on the strain under aerobic conditions, and in this way, adaptive evolution is performed to obtain Escherichia coli with good growth performance and high yield of O-succinyl-L-homoserine. The present invention improves the substrate utilization rate and product tolerance of the strain by using adaptive evolution under aerobic conditions, thereby improving the ability of the strain to produce O-succinyl-L-homoserine and the sugar-acid conversion rate.

Escherichia coli is modified by means of metabolic engineering, a genetically engineered bacterium for high yield of O-succinyl-L-homoserine is obtained, and a construction method of the genetically engineered bacterium comprises the following steps: weakening a synthetic route of an organic acid metabolic byproduct of a chassis strain E.coli W3110 [delta] metI [delta] metJ [delta] thrB [delta] metB; wherein the synthetic route of the organic acid metabolic byproducts is selected from at least one of a formic acid synthetic route, a lactic acid synthetic route and an ethanol synthetic route. The constructed genetically engineered bacterium is applied to microbial fermentation production of the O-succinyl-L-homoserine, effective accumulation of the O-succinyl-L-homoserine is achieved, the sugar acid conversion rate reaches 46.8%, and a foundation is laid for subsequent construction of high-yield O-succinyl-L-homoserine engineering bacteria.

The invention provides an embedded anaerobic ammonia oxidation granular sludge composite material as well as a preparation method and application thereof, and belongs to the technical field of biological sewage treatment. The embedded anaerobic ammonia oxidation granular sludge composite material provided by the invention consists of an inner core and a shell, wherein an inner core comprises anaerobic ammonia oxidation granular sludge and an embedding agent crosslinked with the anaerobic ammonia oxidation granular sludge, a shell comprises a carrier and N-acyl-homoserine lactone signal molecules loaded on the carrier, and preparation raw materials of the carrier comprise a hydrophilic alcohol polymer and a biodegradable high polymer material. In the initial stage of microbial culture, the quorum sensing mechanism of anaerobic ammonia oxidation granular sludge is utilized, and expression of related genes is stimulated through N-acyl-homoserine lactone signal molecules, so that a microbial film is promoted to grow quickly, and the number of florae is increased; meanwhile, the embedded anaerobic ammonia oxidation granular sludge is not easy to lose, and the anaerobic ammonia oxidation reaction activity can be maintained.

The invention discloses recombinant escherichia coli and a method for producing L-methionine by using the recombinant escherichia coli. L-methionine is produced through fermentation culture of recombinant escherichia coli, sulfur source optimization is carried out on a sulfur source culture medium used during fermentation culture, the sulfur source utilization efficiency is improved, and the yield of L-methionine is increased; in the optimization process, generation of hydrogen sulfide in a culture medium is detected by lead acetate test paper, so that metZ genes for expressing and coding O-succinyl homoserine sulfhydrylase are introduced into plasmid pAm to obtain plasmid pAmZ, and the plasmid pAmZ is introduced into strains M2 / pAm and M2-HPBC / pAm to obtain recombinant escherichia coli M2 / pAmZ and M2-HPBC / pAmZ, so that the toxic action of hydrogen sulfide on cells is reduced, and the yield of hydrogen sulfide is increased. The yield of the L-methionine can be increased; according to the present invention, the recombinant escherichia coli M2 / pAmZK and the recombinant escherichia coli M2-HPBC / pAmZK are obtained by further introducing the ppnk gene expressing and coding NAD + kinase into the plasmid pAmZ, and introducing the ppnk gene into the bacterial strains M2 / pAmZ and M2-HPBC / pAmZ to obtain the recombinant escherichia coli M2 / pAmZK and M2-HPBC / pAmZK, such that the intracellular NADPH level is improved, and the L-methionine yield is further improved;

Provided are a microorganism in which activity of an SIRT-type deacetylase protein is weakened; a method for producing O-acetyl homoserine and L-methionine using the same; a composition for producing O-acetyl homoserine, the composition including the microorganism; and use of the microorganism for producing O-acetyl homoserine or L-methionine.

The invention discloses a preparation method of magnetic biochar-quorum sensing composite particles and an application of the magnetic biochar-quorum sensing composite particles in enhancing anaerobic ammonia oxidation activity, magnetic biochar is prepared from magnetic mesoporous silicon oxide nano-particles which are synthesized by loading modified biochar and have a core-shell structure, Pseudomonas aeruginosa with a quorum sensing regulation function is loaded, and the quorum sensing composite particles are prepared into the magnetic biochar-quorum sensing composite particles. The prepared magnetic biochar-quorum sensing composite particles have good chemical stability, biocompatibility and adsorption capacity, can be stably combined with strains, and can be used for enhancing the anaerobic ammonia oxidation activity by applying the composite particles to the process of enhancing the anaerobic ammonia oxidation activity. The pseudomonas aeruginosa secretes homoserine lactone signal molecules to regulate and control the metabolism of anaerobic ammonium oxidation flora in the reactor, and meanwhile, the magnetic biochar is used as a conductive material to accelerate the electron transfer efficiency in the anaerobic ammonium oxidation process, so that the anaerobic ammonium oxidation activity is effectively improved.

The present application relates to: a microorganism in which the activity of a SIRT-type deacetylase protein is attenuated; a method for producing O-acetyl homoserine and L-methionine using the same; a composition for the production of O-acetyl homoserine, which comprises the microorganism; and to the use of said microorganism for the production of O-acetyl homoserine or L-methionine.

The invention provides a recombinant O-succinyl-L-homoserine mercaptotransferase mutant, a coding gene thereof, a recombinant vector containing the coding gene of the mutant, a recombinant genetically engineered bacterium obtained by converting the recombinant vector, and application of the recombinant O-succinyl-L-homoserine mercaptotransferase mutant in preparation of L-methionine. The novel recombinant O-succinyl-L-homoserine mercaptotransferase mutant with high activity and high selectivity is used as a catalyst to be applied to catalysis of O-succinyl-L-homoserine for synthesis and preparation of L-methionine, a catalytic reaction system is optimized, the feed ratio and reaction conditions in the reaction are finely regulated and controlled, and the yield of L-methionine is increased. The recombinant O-succinyl-L-homoserine mercaptotransferase mutant has high enzyme activity under the optimal reaction condition, the catalytic efficiency is further improved, the purposes of reducing cost and improving efficiency are achieved, and the method has important significance in promoting industrial application of biological catalysis preparation of L-methionine.

The present disclosure provides a recombinant Escherichia coli strain modified by metabolic engineering means and a method for producing L-homoserine by using the same. The strain, designated as Escherichia coli having a strain number of 13-XA, is deposited in China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCC No. 25099, dated Jun. 16, 2022. With respect to the chromosome DNA thereof, one or more genes associated with fatty acid metabolism are knocked out or attenuated, and / or a promoter is replaced for enhancement; one or more genes associated with the L-homoserine metabolic pathway are knocked out or attenuated, and / or one or more genes associated with the L-homoserine metabolic pathway are overexpressed or enhanced, and / or one or more genes associated with the L-homoserine metabolic pathway are mutated.

The invention provides a high-yield O-succinyl-L-homoserine engineering bacterium as well as a construction method and application of the high-yield O-succinyl-L-homoserine engineering bacterium. According to the invention, O-succinyl-L-homoserine genetically engineered bacteria with high yield of O-succinyl-L-homoserine are constructed by introducing O-succinyl-homoserine transferase from Vibrio cholera into a chassis strain in an exogenous manner and obtaining a gene mutant metAVc (T242A) of the O-succinyl-L-homoserine transferase, and further increasing the expression of the O-succinyl-L-homoserine transferase by adopting a Trc promoter. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 18.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 105.4 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The invention discloses recombinant escherichia coli capable of increasing the yield of L-homoserine and a preparation method of the recombinant escherichia coli, and belongs to the technical field of biology. By constructing a multi-way glucose transport system, L-homoserine synthesis precursor supply is enhanced, high-energy-consumption genes are knocked out, ATP regeneration is enhanced, ATP availability is improved, an acetic acid conversion way is established, and an L-homoserine degradation way is blocked, so that a metabolic flow of a synthesis way is further enhanced; a pyruvate carboxylase mutant and a designed promoter are introduced to weaken expression of citrate synthase and reconstruct key metabolic nodes, and fed-batch fermentation conditions of the strain in a 5L bioreactor are optimized according to metabolic characteristics of the strain; the genetically engineered bacterium which is clear in genetic background, does not carry plasmids, does not need induction and can stably and efficiently produce the L-homoserine is obtained, the yield of the L-homoserine reaches 141.5 g / L after the L-homoserine is fermented for 56h in a 5L fermentation tank, the saccharic acid conversion rate reaches 40%, and the genetically engineered bacterium has a certain application prospect.

The invention discloses a genetically engineered bacterium for producing homoserine as well as a construction method and application of the genetically engineered bacterium. The method comprises the following steps: enhancing pyc, ppc, EcthrA and tkt genes related to precursor and reducing power supply in the genetically engineered bacteria, and knocking out or weakening genes of branch pathways gltA, dapA, avtA, alaT, pqo, ddh and thrB; modifying and transforming lysE and EcrthA genes of a transport protein system; the method comprises the following steps: introducing an aspartate kinase gene SclysC and a homoserine dehydrogenase Bdhom gene which are heterologous and have feedback resistance; a heterologous NADPH production enzyme is introduced; and cglK, betaP and grpE related to osmotic pressure tolerance in the genetically engineered bacteria are enhanced. The invention discloses the genetically engineered bacterium which is constructed by using the method and has improved yield of L-homoserine and a method for producing L-homoserine and other downstream products by using the genetically engineered bacterium.

The invention provides a diagnostic marker for metabolic syndrome and preclinical stage thereof, and the diagnostic marker or a plasma small molecule metabolism biomarker consists of the following substances: pyrimidine, (S)-beta-aminoisobutyric acid, 3-hydroxybutyric acid, L-serine, guanidine acetic acid, 3-hydroxyisovaleric acid, L-homoserine, L-cysteine, piperidine acid, hypotaurine and creatinine. The beverage is prepared from phenylacetic acid, erythritol, picolinic acid, taurine, pyroglutamic acid, lemonic acid, malic acid, tyramine, isonicotinic acid, o-phosphoethanolamine, L-glutamine, salicylic acid and D-glucose. The invention also provides a kit for detecting the plasma small molecule metabolism biomarker composition, the spectrum peak intensity of the obtained plasma small molecule metabolism biomarker is detected, the risk score of whether each subject is MetS or pre-MetS is calculated, and then whether the subject is MetS or pre-MetS is predicted. According to the invention, efficient and rapid diagnosis of the metabolic syndrome and the preclinical stage thereof is realized.

There is provided peptidic compounds of Formula I, A or B(Rradn6-[linker]-RL-Xaa1-Xaa2-Xaa3-Xaa4-Xaa5-Xaa6-Xaa7-Xaa8-ψ-Xaa9-NH2). Xaa1 is D-Phe, Cpa, D-Cpa, Nal, D-Nal, 2-Nal, or D-2-Nal; Xaa2 is Asn, Gln, Hse, Cit or His. Xaa3 is Trp, Bta, Trp(Me), Trp(7-Me), Trp(6-Me), Trp(5-Me), Trp(4-Me), Trp(2-Me), Trp(7-F), Trp(6-F), Trp(5-F), Trp(4-F), Trp(5-OH), or αMe-Trp. Xaa4 is Ala or Ser. Xaa5 is Val, Cpg, or Tle. Xaa6 is Gly, NMe-Gly, or D-Ala. Xaa7 is His or NMe-His. Xaa8 is Leu or Phe. Xaa9-NH2 is a C-terminally amidated amino acid residue selected from Pro, 4-oxa-L-Pro, Me2Thz, or Thz. ψ represents a peptide bond or reduced peptide bond joining Xaa8 to Xaa9. Rradn6 is 1-5 radiolabeling groups. There is also provided the use of such compounds as imaging agents or therapeutic agents.

The invention relates to the technical field of microorganisms, and discloses an application of a secondary metabolite of phytobacterium plantarum YN-06 in lowering cholesterol, the phytobacterium plantarum YN-06 is preserved in China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation time is March 13, 2025, the preservation number is CCTCC NO: M 2025471, and the classification name is Lactobacillus plantarum YN-06. According to the application of the secondary metabolite of the phytobacterium plantarum YN-06 in lowering cholesterol, the YN-06 has a remarkable cholesterol degradation effect, can produce various beneficial metabolites such as L-beta-homoserine and 5-amino valeraldehyde, can be used for developing fermented dairy products and other functional foods with a cholesterol lowering function, and has a wide application prospect. And new strategies and products are provided for preventing and improving hypercholesteremia and related cardiovascular diseases.

Provided is a method for synthesizing L-glufosinate, comprising the following steps: first making L-homoserine react with acetic acid to form a compound A or a salt thereof; then catalyzing the compound A or the salt thereof by a synthetase to form a compound B; then carrying out chlorination and ring opening on the compound B to form an intermediate compound C; then carrying out an Arbuzov reaction on the intermediate compound C and a phosphine reagent to form a compound D or a salt thereof; and carrying out a hydrolysis reaction on the compound D or the salt thereof regardless of whether the intermediate is separated or not to form L-glufosinate or a salt thereof. The method for synthesizing L-glufosinate combines a chemical synthesis method with an enzyme catalysis method to obtain L-glufosinate having both a high e.e. value and high yield, and has simple preparation steps and controllable cost.

The present invention relates to an enzymatically catalyzed method for producing L-glufosinate or a phosphoester thereof. The method includes reacting an activated L-homoserine HA with a substrate S selected from methylphosphinic acid and esters thereof. Sulfhydrylase enzyme E1 is used for the enzymatic catalysis. The invention makes accessible new substrates for the enzymatic production of L-glufosinate and its phosphoesters.

The present invention relates to a control method for slowing down MBR membrane pollution by using microbial predation to inhibit quorum sensing, and belongs to the technical field of sewage biological treatment. The method comprises the following steps: 1. Cultivating obligate predatory bacteria with Gram-negative bacteria that can secrete signal molecules (N-acyl homoserine lactone) as the main host to obtain a quorum sensing inhibitor; 2. Directly putting the cultivated quorum sensing inhibitor into the MBR reaction pool, the quorum sensing inhibitor will carry out an obligate predation process in the MBR reaction pool; the quorum sensing inhibitor can prey on Gram-negative bacteria that secrete N-acyl homoserine lactone and inhibit quorum sensing. The present invention can effectively reduce the concentration of signal molecules in the MBR reaction pool, so that it cannot reach the threshold of triggering quorum sensing and thus avoid the production of extracellular polymers, so as to inhibit the formation of biofilm from the source, and has a significant control effect on MBR membrane pollution.