112 results about "Tumor hypoxia" patented technology

The invention discloses a gold nanocage-manganese dioxide composite nanoparticle as well as a preparation method and application thereof, and relates to the technical field of nanoparticle photodynamic therapy. The composite nanoparticle comprises a gold nanocage inner core and a manganese dioxide shell layer. The preparation method is characterized in that potassium permanganate is reduced by a one-step reduction method; the manganese dioxide shell layer covers the surface of the gold nanocage, wherein the gold nanocage inner core achieves a photodynamic curative effect under the triggering of near-infrared light; the manganese dioxide shell layer is degraded in tumor microenvironment and releases oxygen, so that the tumor oxygen deficiency is relieved, and the photodynamic curative effect is enhanced; on the other hand, the opto-acoustic and magnetic resonance bimodule imaging can also be realized. The problem of insufficient photodynamic curative effect caused by factors such as tumor oxygen deficiency and photodynamic oxygen consumption of a nanometer photosensitizer used in the prior art is solved. The photodynamic curative effect of the gold nanocage-manganese dioxide composite nanoparticle is obvious; the gold nanocage-manganese dioxide composite nanoparticle can be applied to tumor targeted oxygenation photodynamic diagnosis and treatment integration.

The invention relates to preparation and application of medicine-carrying polydopamine / dendrimer-gold nanoparticles. The preparation comprises the steps of modifying dendrimer-gold nanoparticles to the surfaces of polydopamine nanoparticles, and performing polyethylene glycol modification and anticancer medicine adriamycin loading. Obtained composite nanoparticles having a core-satellite structurecan be subjected to acid response to release minisize satellites, medicine delivery in deep tumors is realized, and medicine tolerance generated by tumor hypoxia is relieved; and adriamycin is released at a fixedpoint under internal (acid and hydrogen peroxide) and external (near infrared light) stimulation. The obtained composite nanoparticles have favorable biocompatibility, can realize optoacoustic diagnosis and photothermal treatment on tumor locations, and what's more, can realize remarkable restraining effects on tumor growth. Through the adoption of the method disclosed by the invention, the treatment effects of traditional chemical medicines can be notably improved, more comprehensive three-dimensional tumor treatment is realized, and a new thinking is provided for diagnosis and treatment of malignant tumors.

The invention provides agents that target carbonic anhydrase, which can be used as imaging agents or therapeutic agents. The agents can be used to image tumor hypoxia as well as other physiological processes in a subject.

The invention discloses a composite nanoparticle for sensitizing tumor radiotherapy and a preparation method and application of the composite nanoparticle. The composite nanoparticle comprises proteinand bismuth selenide and manganese dioxide which grow on the protein. The preparation method comprises the steps that a manganese salt solution is added into a aqueous dispersion of bismuth selenide-protein nanoparticles, after uniform mixing is carried out, a strong alkaline solution is added, the pH value is adjusted to alkaline, a heating temperature control reaction is carried out, ultrafiltration and washing are carried out, and the composite nanoparticle is obtained. The composite nanoparticle has the advantages that the water dispersibility and biocompatibility are good, radiotherapy is sensitized by increasing the local radiation dosage through bismuth selenide and improving tumor hypoxia through manganese dioxide, and computed tomography, magnetic resonance and photoacoustic multimode imaging angiography can be achieved simultaneously; the preparation method of the composite nanoparticle is simple, convenient and practical, the condition is mild, the controllability is good,and the implementation and promotion are easy; the composite nanoparticle can achieve the integration of targeted radiotherapy sensitization, diagnosis and treatment of the tumor, and has a broad application prospect in the fields of nano-medicine, disease diagnosis, tumor treatment and the like.

The invention relates to the field of medicine, in particular to Rakicidins compounds Rakicidin B1. Pharmacodynamic tests prove that the Rakicidin B1 can be applied to treatment of diseases such as diarrhea, digestive tract inflammation, oral inflammation, skin acne and the like caused by solid tumors such as stomach cancer, liver cancer, breast cancer, pancreatic cancer, intestinal cancer in a tumor hypoxia condition as well as anaerobic bacteria.

Methods and compositions for enhancing the ability of hypoxia-activated bioreductive agents to kill tumor cells within solid tumors are provided. Local regions of hypoxia are created within a tumor, or within a region containing a tumor, resulting in enhanced activation of hypoxia-activated bioreductive agents (e.g. tirapazamine) within the local region. The activated hypoxia-activated bioreductive agents kill tumor cells in the hypoxic region by catalyzing DNA stand breakage within the tumor cells. Because the activity is localized, side effects that typically occur as a result of systemic administration of bioreductive agents are reduced.

The invention provides a preparation method of Cu2MoS4 nano-enzyme, and the method comprises the following steps: mixing cuprous oxide, an organic carbon source, an inorganic molybdenum source and anorganic sulfur source in an organic solvent, and carrying out high-temperature calcination to obtain the hollow Cu2MoS4 nano-enzyme. Compared with the prior art, the Cu2MoS4 nano-enzyme prepared by the invention has catalase activity and greatly relieves tumor hypoxia, and meanwhile, the nano-enzyme also has peroxidase activity and can consume glutathione overexpressed in a tumor, so that an antioxidant defense system of the tumor is destroyed, and the chemokinetic treatment efficiency is enhanced; two pairs of redox couples exist, the redox couples can react with hydrogen peroxide to generatehigh-toxicity hydroxyl radicals, a remarkable chemical and dynamic curative effect is achieved, meanwhile, the nano-enzyme has strong absorption in the whole near-infrared area, and photo-thermal therapy can be achieved; and under laser excitation, superoxide anion free radicals can be generated and used for photodynamic therapy.

The invention relates to a compound for detecting tumor hypoxia disclosed as Formula I, a preparation method and application thereof, and a kit containing the compound. The groups in the formula are disclosed in the specification. The compound can be used for detecting hypoxia of tumor tissues, can respond to changes of partial oxygen pressure and reducibility of partial tissues, and provides conditions for early detection of cancers. The compound has the advantages of novel structure, simple synthesis process, high detection sensitivity, high fluorescence enhancement multiple, no trauma and the like, and can be used for cell detection and even living body detection.

The invention provides a nitroreductase-specific fluorescent probe and the preparation thereof and a reagent for applying the nitroreductase-specific fluorescent probe to tumor-targeted fluorescence imaging and monitoring of tumor hypoxia. The reagent is connected by tumor biomarker identifying groups (sensor), tumor targeted groups (target) and fluorescent groups (dye) via chemical bonds. The reagent can be used in the tumors with hypoxic microenvironment and high expression of nitroreductase based on tumor hypoxia and the high expression of nitroreductase in hypoxic tumors. Combined with a fluorescence imager, the reagent can be used in tumor targeted fluorescence imaging, tumor hypoxia monitoring and the monitoring of tumor metastasis, etc. The reagent realizes high sensitivity and high specificity in application and provides an effective tool for medical study of cancer and clinical monitoring and treatment of tumor metastasis, thereby having a good application prospect in tumor-targeted fluorescence imaging, tumor hypoxia monitoring and monitoring of tumor metastasis.

The invention belongs to the field of biomedicine, and relates to a 3D co-culture model based on a tumor cell and a tumor-associated fibroblast. The tumor cell is labeled with a green fluorescent protein, the tumor-associated fibroblast is labeled with a red fluorescent protein, and the two cells and a prepared methylcellulose gel medium are used to construct the 3D co-culture model. The 3D modelprepared in the invention simulates the anaerobic environment of in-vivo tumors, and the tumor cell and the tumor-associated fibroblast are co-cultured to construct the mutual promotion effect of thetwo cells in the in-vivo tumor microenvironment. The 3D co-culture model formed by the tumor cell and the tumor-associated fibroblast simulates the anaerobic environment of the in-vivo tumor, providesa similar effect to the in-vivo tumor microenvironment, and has application prospects in basic researches and clinical drugs.

A device and a method to measure the concentrations of oxygenated and deoxygenated hemoglobin in tissue around a tumor via near-infrared (NIR) spectroscopy with a photonic mixer device (PMD) is described.

The invention discloses a synthesis method and application of an anthraquinone polypyridine ligand and anthraquinone-ruthenium polypyridine complex. The anthraquinone-ruthenium polypyridine complex has good inhibition activity on the growth of hypoxic cells, and generates obvious fluorescence through reduction in the hypoxic cells; and thus, the compound is a novel biological reduction type hypoxic cell anti-cancer drug and a specific fluorescence probe as well as a drug with application value and taking tumor hypoxia as a target point and a fluorescence probe.

The invention discloses an application of gold platinum thermometal nano particles on preparation of cell immunotherapy accelerant. According to the application, the hydrogen peroxide enzymatic activity of the gold platinum thermometal nano particles is used, the gold platinum thermometal nano particles can react with hydrogen peroxide in the tumour hypoxia microenvironment and generate oxygen andhave good biocompatibility, the gold platinum thermometal nano particles are combined with cell immunotherapy and used as accelerant of cell immunotherapy, so that the tumour hypoxia situation is improved, inhibition of the tumour hypoxia environment to the killing capacity of the immune cell is lowered, accordingly, the killing effect of the immune cell to the hypoxia tumour cell is remarkably improved, and the gold platinum thermometal nano particles have wide application prospects on tumour immunotherapy.

The invention discloses a nitro imidazole xanthogenate complex marked by TcO with the formula of TcO (NMXT)2; in the formula, NMXT represents a compound containing nitro imidazole xanthogenate. The complex adopts Tc(V)O as a central nucleus and has a geometric configuration with the shape of an irregular square pyramid, wherein O atom in Tc=O bond is positioned at the top point and four sulphur atoms provided by two xanthogenate ligand molecules are positioned at the four points of the bottom surface. By the technical steps of synthesis of ligand NMXT and preparation of TcO (NMXT)2 complex, the TcO (NMXT)2 complex is obtained. The nitro imidazole xanthogenate complex has high radiochemical purity and good stability, has higher uptake value and good detention through in tumor and higher ratio of tumor / muscle, is widely applied to tumor imaging of human body and animal organs and is a novel tumor hypoxia imaging agent which is conveniently promoted and applied.

The present invention provides a method of increasing oxygen content in tumors by administration of stressed cells, or inducing formation of stressed cells in vivo. Such treatment allows for increased efficacy of drugs, or radiation therapy, which normally would not be fully effective due to tumor hypoxia.

The invention relates to a preparation method and application of polyethylene glycol polydopamine-gold nanoparticles. The preparation method comprises the steps of preparing the polydopamine nanoparticles through a one-pot method; modifying the surface of the polydopamine through polyethylene glycol; loading gold nanoparticles by utilizing the chelating ability of polydopamine; and performing performance evaluation on the obtained final product. The method disclosed by the invention is simple and easy to implement, and the prepared polyethylene glycol polydopamine-gold nanoparticles have goodmorphology uniformity and oxygen production capacity and have potential tumor hypoxia relieving capacity and photo-thermal treatment capacity.

The invention discloses a cell co-culture microfluidic chip capable of realizing an oxygen concentration gradient. The cell co-culture microfluidic chip comprises a hypoxia channel generating an oxygen gradient, and a cell culture unit composed of five parallel channels, wherein the hypoxia channel has two liquid inlets and one liquid outlet, oxygen in the channels is absorbed by a chemical reaction generated by liquid mixing, and thereby the oxygen gradient is generated in the cell culture unit; and hexagonal micro-pillar arrays are arranged between the cell culture channels, a liquid flowingthrough can produce surface tension and cannot leak to adjacent channels, and thereby multiple cells can be cultured in different channels within the chip. The invention also provides an applicationof the microfluidic chip. The microfluidic chip can construct a rapid and stable oxygen concentration gradient in vitro, different cells are co-cultured in the parallel channels in the chip, and the microfluidic chip is used for simulation of tumor hypoxia micro-environment in vitro.

The invention discloses a new function of cyanobacteria naturally existing in nature, and aims to provide a photosensitizer with a simple preparation process, overcome a tumor hypoxia microenvironmentthrough oxygen self-production by utilizing the characteristic that cyanobacteria generates oxygen through photosynthesis after being illuminated, and target tumors to play an effective photodynamicrole. The in-vitro experiments prove that the cyanobacteria can effectively inhibit the growth of 4T1 cells after being irradiated by laser with a wavelength of 660nm. The photosensitizer is simple inprocess step and low in cost, only a large amount of cyanobacteria needs to be simply multiplied and cultured, and oxygen can be supplied to the photosensitizer by providing specific illumination, sothat tumor hypoxia is overcome, the photodynamic therapy effect is achieved, and the photosensitizer capable of generating oxygen by itself and having industrial practical value is developed.

The invention relates to the technical field of biomedicines and relates to a hypoxia responsive liposome preparation, and preparation and applications thereof. In particular, the invention relates to hypoxia responsive liposome-AQ4N-dihydroporphin e6 and preparation and application thereof, and especially, application thereof in enhancement of killing cells in tumor hypoxia zones in photodynamics therapy. The liposome preparation is composed of a liposome, AQ4N and dihydroporphin e6, has uniform particle size and is excellent in dispersion in water. The particle size of the liposome preparation is mainly about 100 nm in water. The liposome preparation can carry photosensitive molecules and dimethyldiguanide and is an ideal medicine carrier, and also has excellent in-vivo pharmacokinetics behavior. A test proves that the liposome-AQ4N-dihydroporphin e6 preparation is very-highly enriched at tumor parts, so that after the photodynamics therapy, the preparation can cause a more serious hypoxia status in the tumor zone, thereby improving the killing effect on the hypoxia cells of the AQ4N and obtaining excellent synergistic therapy effects on tumor.

The invention discloses a nano-drug carrier Au / MnO2. The nano-drug carrier is a core-shell structure with the gold nanorod AuNRs as the core and mesoporous manganese dioxide MnO2 as the shell. A preparation method comprises the following steps: firstly preparing AuNRs by a seed growing method, and then coating the surface of AuNRs with MnO2 having the mesoporous structure by the utilization of a reaction between potassium permanganate and anhydrous ethanol in an alkaline or neutral environment. Light and heat stability of AuNRs is raised, and the drug loading purpose is also achieved. By combining MnO2 and AuNRs, the synergistic treatment effect can be effectively enhanced. The original tumor is further killed. Through the reaction with GSH, H<+> and H2O2 existing in the tumor microenvironment for decomposition into water and oxygen, tumor hypoxia is alleviated and the tumor microenvironment is improved, growth of distal tumor is effectively inhibited. It shows that a MnO2-based hybridmaterial has a huge application potential in the field of tumor treatment.

The invention discloses a new function of cyanobacteria. Under irradiation of specific wave length lasers, and through photosynthesis, oxygen is generated, the condition of oxygen deficiency caused bytumors can be improved, and the problem of reverse tolerance of chemicals, such as adriamycin can be effectively solved. The cyanobacteria is subjected to photosynthesis in light to generate oxygen,and under the aerobic condition, hypoxia factors are reduced for tumor cells, so that multidrug resistance genes can be restrained, expression of P-glycoprotein is restrained to restrain exocytosis ofcancer cells on adriamycin, the reverse tolerance of the cancer cells on the reverse tolerance is overcome, and the antitumor treatment effect can be increased.

The invention provides a cyanobacterium of a living drug. Oxygen is generated by means of photosynthesis under laser radiation to improve tumor hypoxia, so that the tumor microenvironment is changed,an oxygen-increasing tumor therapeutic effect is achieved, and novel selection is provided for cancer treatment. An in vitro experiment verifies that the cyanobacterium can generate oxygen infinitelyunder a condition of 660 nm laser radiation. An in vivo experiment verifies that the tumor hypoxia condition is improved obviously by tail vein injection of the cyanobacterium into a mouse body and radiation on mouse tumors by 660 nm laser.

The invention discloses a photodynamic therapy agent for relieving tumor hypoxia as well as a preparation method and application thereof, and belongs to the technical field of medicines. The photodynamic therapy agent is a metal-organic framework nano composite material loaded with O2. The metal-organic framework nano composite material is metal-organic framework nano particles Zr-TCPP (Fe), the interiors of nano frameworks of the nano particles Zr-TCPP (Fe) are modified with perfluorooctanoic acid, and meanwhile glucose oxidase GOx is further adsorbed in pore channels of the nano particles Zr-TCPP (Fe). According to the invention, a drug delivery system capable of catalyzing tumor to endogenously generate O2 is designed while O2 is delivered to tumor tissue, so that the hypoxic environment can be remarkably improved, and the photodynamic therapy effect is enhanced. In addition, the synthetic steps of the therapy agent are relatively simple, and the yield is relatively high; and a synthetic method is simple and relatively low in cost, so that the method is suitable for large-scale production.

The invention provides a composite nano-liposome and application thereof. The composite nano-liposome is prepared from soybean lecithin, folic acid-modified distearoyl phosphoethanolamine-polyethyleneglycol 2000, cholesterol, a photosensitizer, chemotherapeutic drugs and catalase. A system utilizes the liposome as a nano drug carrier system, the solubility of the drugs is improved, the toxic andside effects are reduced, and the targeting ability to a tumor site is increased. According to the composite nano-liposome, the photosensitizer and an anticancer drug are encapsulated together, and itis found that the two drugs have a good synergistic therapeutic effect through cell and in-vivo antitumor experiments; in addition, the catalase is loaded to alleviate tumor hypoxia caused by the photodynamic therapy, and the inhibition to tumors is enhanced; and folic acid-modified phospholipids are used for realizing active targeting of the tumors, and the anti-tumor effect is greatly enhanced.

The invention provides a HIFU biological targeting synergist and a preparation method thereof. The HIFU biological targeting synergist is specifically formed by combining bifidobacterium and a HIFU synergist. The bifidobacterium is a probiotic beneficial to the human health and high in biosafety, the obligative anaerobic characteristic of the bifidobacterium can target a tumor hypoxia area, the bifidobacterium is directionally proliferated in the tumor hypoxia area, and the bifidobacterium has an anti-tumor effect. The HIFU biological targeting synergist can more accurately target tumor tissue, and can improve the HIFU energy deposition and prolong the residence time, thereby achieving the purpose of improving HIFU targeted tumor ablation.

The invention discloses a porphyrin-based metal organic framework nano-carrier with a sonodynamic combined starvation therapy anti-tumor function and a preparation method of the porphyrin-based metal organic framework nano-carrier. The preparation method comprises the following steps: dissolving a porphyrin-based ligand and metal salt in a solvent, and heating to obtain porphyrin-based metal organic framework particles; and dispersing the porphyrin-based metal organic framework particles into water, adding high-valence metal acid or high-valence metal salt and a reducing agent, and mixing with a cell membrane and glucose oxidase to obtain the cell membrane modified porphyrin-based metal organic framework nano-carrier loaded with the metal nanoparticles and the glucose oxidase. The nano-carrier can enhance the accumulation at the tumor site and improve the tumor targeted hunger treatment effect; and meanwhile, tumor hypoxia is relieved, sonodynamic reaction substrates are increased, and the sonodynamic effect is improved. The preparation process is simple and controllable, the material source is wide, and scale amplification can be realized; and in vivo, good stability and tumor sonodynamic and hunger treatment combined treatment effect are achieved, and good application prospects are achieved in the field of tumor treatment.

The invention provides a vermiculite nanosheet and a preparation method thereof. The preparation method comprises the steps: adding vermiculite into an alkali metal salt solution modifier, carrying out intercalation treatment, and stripping to obtain the vermiculite nanosheets, wherein the alkali metal salt modifier with the concentration of 0.1-10 mol / L is added into each milligram of vermiculite. The vermiculite nanosheet provided by the invention not only can be used as a nano reinforcing material in industries such as papermaking, coating, paint, plastic, rubber and the like, but also can be used as an oxygen self-supply platform to generate oxygen and various active oxygen, and is effectively used for preparing biomedical photodynamic therapy medicines, overcoming tumor hypoxia and enhancing the effect of photodynamic therapy. The preparation method of the vermiculite nanosheet provided by the invention is simple in process, green and environment-friendly, and convenient for industrial application.

The invention provides a CaO2@DOX@ZIF-67 nanometer material and a preparation method and application thereof. The nanometer material is prepared through the steps of loading adriamycin on the surfaceof nanometer CaO2 through coordination effects, then adsorbing metal ions through the adriamycin on the surface of the CaO2, and then enabling the metal ions and bridging ligands to be subjected to anin situ reaction. After a nanometer reactor CaO2@DOX@ZIF-67 is transported to a tumor position through a blood circulating system, through EPR effects, the nanometer reactor CaO2@DOX@ZIF-67 is enriched in tumor tissue and taken in by tumor cells, ZIF-67 is slowly decomposed to release out Co<2+> in subacid environment, besides, CaO2@DOX is decomposed to release out DOX, and then CaO2 and water are subjected to a reaction to generate O2 and H2O2, so that the oxygen concentration of the tumor tissue can be increased, tumor hypoxia is overcome, and sensibility of hypoxia tumor cells to chemotherapy medicines is strengthened. The Co<2+> or Fe<3+> can catalyze H2O2 to generate a Fenton like reaction, massive .OH can be generated, tumor cell DNA can be destructed through oxidization, and deathof the tumor cells is promoted.