Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of of polyethylene glycol polydopamine-gold nanoparticles

A kind of polyethylene glycol polydopamine, polydopamine nanotechnology, applied in nanotechnology, nanotechnology, nanomedicine and other directions, can solve the problem of not finding polyethylene glycol polydopamine-gold nanoparticles, etc., to achieve good uniformity of morphology Sexuality and oxygen production capacity, mild effect of the preparation process

Pending Publication Date: 2020-09-29
SOUTHWEST UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Searching domestic and foreign literature and patents on polydopamine nanoparticles for cancer treatment found that: before the completion of the present invention, the preparation of polydopamine-gold nanoparticles based on polyethylene glycol and its use in tumor hypoxia relief have not been found. and research reports on photothermal therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of of polyethylene glycol polydopamine-gold nanoparticles
  • Preparation method and application of of polyethylene glycol polydopamine-gold nanoparticles
  • Preparation method and application of of polyethylene glycol polydopamine-gold nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Add 90 mL of ultrapure water, 40 mL of absolute ethanol, and 2-3 mL of ammonia water (28%-30%) into a 250 mL round-bottomed flask in sequence, then place the flask on a magnetic heating stirrer and heat and stir in a water bath for 10 min. A homogeneous solution was formed. 10 mL of 50 mg / mL dopamine hydrochloride (DA) solution was quickly added to the above solution, and reacted for 12-24 h to obtain a dispersion of polydopamine nanoparticles. Then centrifuge (16000 r / 10 min) and wash with water three times to obtain polydopamine PDA nanoparticle dispersion, which is stored at 4°C.

[0032] The results of the laser particle size analyzer (DLS) test showed the hydrodynamic size and uniformity of the obtained polydopamine (PDA) nanoparticles. Refer to the attached figure 1 . The average size of polydopamine (PDA) nanoparticles is 83.64±24.94 nm, and the monodispersity is 0.13±0.05.

Embodiment 2

[0034] Take 2.222 mL of polydopamine nanoparticle dispersion from Example 1, add 11.778 mL of ultrapure water to 2.222 mL of polydopamine nanoparticles (4.5 mg / mL), stir on a magnetic stirrer, and then add 4 mL 50 mM tris buffer, and finally 2 mL of 10 mg / mL mPEG-NH 2 solution was added to the solution. After reacting for 24 h, centrifuge (16000 r / 10 min) and wash with water three times to obtain PDA-PEG nanoparticles, which were stored at 4 °C.

[0035] 1 The H NMR spectrum indicates the chemical environment and type of hydrogen atoms in each component. Refer to the attached figure 2 . 1 The H NMR spectrum shows that a new peak appears at about 3.5 ppm compared with PDA NPs, which can be attributed to the −CH 2 − Proton-related chemical shifts, indicating successful modification with PEG.

Embodiment 3

[0037] Take 0.769 mL of PDA-PEG nanoparticles (6.5 mg / mL) prepared in Example 2, add 8.758 mL of ultrapure water and stir on a magnetic stirrer, then add 0.3 mL of chloroauric acid solution (10 mg / mL) After 15 min, 0.173 mL of sodium borohydride solution (5 mg / mL) was added. After continuing to stir for 2 h, the product was centrifuged (8000 r / 10 min) and washed with water. Thus, polyethylene glycol polydopamine-gold nanoparticles PDA-PEG-Au can be obtained.

[0038] Laser particle size analyzer (DLS) test results show the hydrodynamic size and uniformity of the obtained nanoparticles. Refer to the attached image 3. The average size of PDA-PEG-Au nanoparticles is 107.60±10.52 nm, and the monodispersity is 0.12±0.06.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
sizeaaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method and application of polyethylene glycol polydopamine-gold nanoparticles. The preparation method comprises the steps of preparing the polydopamine nanoparticles through a one-pot method; modifying the surface of the polydopamine through polyethylene glycol; loading gold nanoparticles by utilizing the chelating ability of polydopamine; and performing performance evaluation on the obtained final product. The method disclosed by the invention is simple and easy to implement, and the prepared polyethylene glycol polydopamine-gold nanoparticles have goodmorphology uniformity and oxygen production capacity and have potential tumor hypoxia relieving capacity and photo-thermal treatment capacity.

Description

technical field [0001] The invention belongs to the field of preparation of therapeutic agents for cancer cells, and in particular relates to a preparation method and application of polyethylene glycol polydopamine-gold nanoparticles. Background technique [0002] In recent years, the incidence of malignant tumors has gradually increased and has become an important killer affecting human health. At present, there are outstanding problems in the traditional cancer diagnosis and treatment methods used in clinical practice, and they are facing increasingly severe challenges. There is an urgent clinical need and great practical significance to develop a method with less toxic side effects and good cancer treatment effect. The development of nanotechnology provides a new great opportunity for the effective treatment of malignant tumors. Compared with traditional clinical methods, nanoparticle-based therapeutic methods show better tissue specificity and biocompatibility, and hav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/60A61K47/69A61P35/00B82Y5/00B82Y20/00B82Y40/00C08G73/06
CPCA61K41/0052A61K47/60A61K47/6923A61P35/00B82Y5/00B82Y20/00B82Y40/00C08G73/0672
Inventor 刘辉张秋叶
Owner SOUTHWEST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com