Selenium-enriched bacillus bifidus micro-capsules

A bifidobacteria and microcapsule technology, applied in the field of microbial preparations, can solve the problems that microcapsules are not resistant to gastric acid and bile salts, the device efficiency is not easy to scale up production, etc. rate, and the effect of prolonging the storage time

Inactive Publication Date: 2019-04-12
JIANGSU DAYSEBIOTECH LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of preparing probiotic microcapsules is not easy to scale up production due to the influence of device efficiency, and the prepared microcapsules are not resistant to gastric acid and bile salts

Method used

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  • Selenium-enriched bacillus bifidus micro-capsules
  • Selenium-enriched bacillus bifidus micro-capsules
  • Selenium-enriched bacillus bifidus micro-capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 selenium-enriched bifidobacteria

[0032] The 16S rDNA nucleotide sequence of Bifidobacterium longum DD98 (Latin name Bifidobacterium longum) used in the present invention is shown in SEQ ID No: 1. The bifidobacterium is preserved in the China General Microorganism Culture Collection Management Center (No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101), and the preservation number is CGMCC No. 16573.

[0033] The main components of the RCM medium are beef extract, peptone, yeast powder, glucose, starch, sodium chloride, sodium acetate, L-cysteine ​​hydrochloride, and the content of each component can be adjusted according to the conventional knowledge in the art.

[0034] Preferably, every 10 parts of RCM medium is composed of: 0.1 part of beef extract, 0.05 part of peptone, 0.03 part of yeast powder, 0.05 part of glucose, 0.01 part of starch, 0.05 part of ...

Embodiment 2

[0038] Example 2 Preparation of selenium-enriched bifidobacterium microcapsules

[0039] Mix sodium alginate solution, selenium-enriched bifidobacterium sludge and calcium carbonate powder evenly, then add the above mixed solution drop by drop into soybean oil containing emulsifier, stir, and then add Soybean oil with glacial acetic acid, stir, add emulsifier solution therein, stand still after stirring, until the microcapsules sink to the bottom of the water phase, absorb the oil phase, and centrifuge the water phase (3800r / min, centrifugal 2min) to obtain microcapsules , and then use the washing medium (1% emulsifier solution) to wash the microcapsules twice, remove the residual oil phase, and finally wash twice with sterile saline to obtain uncoated selenium-enriched bifidobacterium microcapsules.

[0040] According to Table 1, each process parameter is screened

[0041]

[0042]

[0043] The above research results show that:

[0044] 1. When Tween 80 is used as an ...

Embodiment 3

[0055] Embodiment 3 microcapsule coating

[0056] The prepared uncoated bifidobacterium selenium-enriched microcapsules were resuspended in a certain volume of purified water, then resuspended in 0.2% coating material solution and stirred, and after the coating was completed, the microcapsules were collected by centrifugation, and used The bacteria were washed twice with normal saline to obtain the coated selenium-enriched bifidobacteria microcapsules. Refer to Table 2 to investigate the effects of different conditions on the embedding rate and particle size during the coating process.

[0057] Table 2 is the effect of different conditions on the embedding rate and particle size in the microcapsule coating process

[0058]

[0059]

[0060] in conclusion:

[0061] 1. Although the particle size of the microcapsules is increased after coating with chitosan, the embedding rate is significantly higher than that of whey protein and polylysine, so chitosan is selected as the...

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Abstract

The invention discloses selenium-enriched bacillus bifidus micro-capsules. The selenium-enriched bacillus bifidus micro-capsules are prepared by adopting a method comprising the following steps: uniformly mixing a sodium alginate solution with selenium-enriched bacillus bifidus thallus and calcium carbonate powder so as to obtain a mixture; dropwise adding the mixture into vegetable oil containingan emulsifier, and carrying out stirring so as to obtain an emulsion; sequentially adding vegetable oil containing glacial acetic acid and an emulsifier solution into the emulsion, carrying out stirring, and allowing standing; and then, separating oil phase so as to obtain aqueous phase, and centrifuging the aqueous phase so as to obtain the selenium-enriched bacillus bifidus micro-capsules. By screening reagents, materials and dosage ratio adopted in micro-capsule preparing, coating and freeze-drying processes, embedding rate of the selenium-enriched bacillus bifidus micro-capsules disclosedby the invention are improved. The embedding rate of the selenium-enriched bacillus bifidus micro-capsules is up to 98% while the particle size is smaller than 200 microns and the freeze-drying survival rate is up to 85%. According to normal-temperature stability results, viable number of the selenium-enriched bacillus bifidus micro-capsules is still about 10<8> cfu/g within 180 days.

Description

technical field [0001] The invention belongs to the field of microbial preparations, in particular to a selenium-enriched bifidobacterium microcapsule. Background technique [0002] Bifidobacterium is one of the important physiological probiotics in the human intestinal tract, which is very beneficial to human health. It mainly has the functions of regulating intestinal flora, anti-oxidation, and enhancing body immunity. Selenium-enriched bifidobacteria refer to A selenium-enriched probiotic obtained by adding selenium to the culture medium of bifidobacteria can combine the effects of selenium and probiotics. In the previous published patents (CN107164295A, CN108721337A), the specific Preparation process and outstanding efficacy in preventing enterotoxicity of tumor chemotherapy. Since bifidobacteria are strictly anaerobic Gram-positive bacteria that grow between pH 4.5 and 8.5, they are sensitive to external adverse environments such as oxygen, humidity, temperature, and g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/52A23C9/152A23C9/13A23L33/135
CPCA23C9/13A23C9/152A23L2/52A23L33/135
Inventor 纪瑞陈代杰邹振荣邵雷吴佳慧谭俊张骏梁张旭东王桃周艳
Owner JIANGSU DAYSEBIOTECH LTD
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