5279 results about "Centrifugation" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to recombinant escherichia coli containing a PDRN sequence and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing a PDRN solution; s2, complementing the double strands of the PDRN into blunt ends; s3, constructing a recombinant plasmid; s4, carrying out mixed culture on a product obtained in the step S3 and escherichia coli DH5alpha; s5, screening the recombinants; s6, carrying out PCR (Polymerase Chain Reaction) amplification, and recording a strain from a fragment with the length of more than 200bp; s7, preparing a seed solution by adopting the strain prepared in the step S6; s8, strain fermentation; and S9, centrifugally collecting thalli. The method comprises the following steps: constructing a recombinant plasmid containing a PDRN fragment, introducing the recombinant plasmid into Escherichia coli, subsequently culturing the recombinant Escherichia coli to copy DNA in the Escherichia coli, extracting a purified exogenous gene fragment to obtain a strain containing a single fragment PDRN sequence, and getting rid of dependence on raw materials during subsequent extraction.

The invention discloses a preparation method of an MOF glucose detection system, ZIF is used as a metal organic framework, glucose oxidase (GOx) is loaded in the ZIF, the preparation method comprises the following steps: S1, water is added into a reaction bottle in advance, then metal ions, enzyme and organic ligands with the molar ratio of (100-1500): (1-10): (1-150) are added into the reaction bottle in advance, and the reaction bottle is filled with water; the method comprises the following steps: S1, adding 0.1-0.5% (by weight) of ammonia water into a reaction bottle, S2, mixing and reacting at room temperature for more than 6 hours, and S3, centrifuging by a centrifugal machine, and removing supernate to obtain solid precipitate of the GOx-MOF material loaded with glucose oxidase. The invention also discloses an application of the MOF glucose detection system in saliva glucose content detection. The method can be applied to glucose detection of oral saliva, and even if the glucose content is extremely low, the glucose concentration in the saliva can be efficiently and accurately detected.

The invention discloses a preparation method of cod skin collagen peptide with high ACE (angiotensin converting enzyme) inhibitory activity, which comprises the following steps: firstly, scaling cod skin, cleaning, cutting into small pieces, pretreating with NaOH and n-butyl alcohol to remove impure protein and certain lipid substances, preparing cod skin gelatin by a hot water extraction method, and then adding the cod skin gelatin into the cod skin collagen peptide to obtain cod skin collagen peptide; the cod skin collagen peptide with high ACE inhibitory activity is prepared by using cod skin collagen as a raw material, performing enzymolysis on the cod skin collagen peptide sequentially by using a double-enzyme distribution enzymolysis method, performing enzyme deactivation and centrifugal treatment after enzymolysis, and performing membrane separation on enzymolysis supernate. The whole enzymolysis process is more thorough by adopting a double-enzyme distribution enzymolysis method, more small molecule peptides can be obtained, cod skin collagen peptide with high ACE inhibitory activity can be further enriched by combining a membrane separation method, the utilization degree of cod skin resources is effectively improved, and the cod skin collagen peptide has a relatively wide application prospect.

Provided are a double-cavity micro-fluidic chip having a reaction cavity and a test cavity and used for nucleic acid test, and a test method thereof. A fluid channel is provided between the reaction cavity and the test cavity, and only one exhaust channel is provided. The combination of the fluid channel and the exhaust channel can automatically provide resistance for a sample entering the test cavity from the reaction cavity, thereby achieving a controllable transfer of the sample between the reaction cavity and the test cavity. A gravity micro-fluidic chip and a centrifugal micro-fluidic chip are designed. The gravity micro-fluidic chip has a multi-turning circuitous fluid channel of which the diameter is smaller than that of a sample injection channel, such that under the action of gravity, the sample can flow into a reaction cavity from a sample adding region but cannot flow into the fluid channel, and then under pressurization by a simple pressurization device, the sample can enter a test cavity from the reaction cavity. The centrifugal micro-fluidic chip provides an exhaust channel branched from a fluid channel, and makes the exhaust direction opposite to a rotary centrifugal direction and the position of an exhaust opening higher than that of the fluid channel; therefore, by combining the principle of internal and external balance of the atmospheric pressure and the centrifugal driving performance, the centrifugal micro-fluidic chip effectively controls the flowing of a gas and a liquid in reaction cavities and test cavities, so as to effectively control the amount of the sample entering each cavity, so that multiple nucleic acid tests can be completed simply by means of one or two times of centrifugation. In addition, for a chip which only has a reaction cavity, an exhaust channel is connected to a liquid storage cavity, and a gravity-driving chip and an asymmetric pressure-driving chip are designed, thereby effectively avoiding aerosol pollution.

The invention discloses a multifunctional composite material for mineralizing and storing CO2 based on fly ash and a preparation method of the multifunctional composite material. The material is prepared from high-activity fly ash subjected to plasma activation treatment, a nanoscale metal oxide catalyst, a metal organic framework, a functional organic ligand, an intelligent pH regulator, a solid suspension stabilizer and water. The preparation method comprises the steps of suspension preparation, high-pressure CO2 mineralization reaction in a reaction kettle, centrifugal separation and freeze drying treatment. The method is simple, the raw material cost is low, the prepared multifunctional composite material not only can remarkably improve the storage efficiency of CO2, but also can ensure that a stable carbonate product is generated, and the carbonate product has high specific surface area and excellent chemical stability, can be widely applied to the fields of high-end building materials, energy storage materials and fine chemical engineering, and has wide application prospects. And meanwhile, the high-added-value utilization of the industrial by-product fly ash is realized, and the dual purposes of reducing carbon emission and improving the utilization rate of solid wastes are achieved.

The invention discloses an anti-EV71 virus activity buthus martensii toxin polypeptide as well as a preparation method and application thereof, and belongs to the technical field of antiviral preparations. A scorpion tail electricity-saving stimulation method is adopted to obtain crude scorpion venom, and centrifugation and freeze drying are performed to obtain scorpion venom freeze-dried powder; dissolving the scorpion toxin freeze-dried powder, filtering, removing sediment, carrying out ultrafiltration centrifugation, and separating to obtain the anti-EV71 virus activity scorpion toxin polypeptide. According to the present invention, the scorpion martensii toxin polypeptides S-I (lt; 3kDa) and S-II (3-10 kDa) obtained from the scorpion martensii have good EV71 virus resistance; under the same concentration, the relative molecular mass is lt; compared with the prior art, the scorpion margaritae toxin polypeptide with the molecular weight of 3kDa has better anti-EV71 virus activity, has no toxic effect on cells, has the potential of being used as an anti-EV71 virus drug, and provides a theoretical basis for biomedical research and clinical application of the scorpion margaritae toxin polypeptide.

The invention provides a carbon powder particle distribution real-time analysis method based on image processing, and the method comprises the steps: applying an adjusted gray threshold parameter to the processing and analysis of a carbon powder particle image, and carrying out the edge extraction, geometric feature extraction and particle size distribution analysis of carbon powder particles again, the adjusted analysis result is used as a basis for regulating and controlling a carbon fiber recovery process; establishing a quantitative relationship between the particle size distribution of the carbon powder particles and the centrifugal rotation rate of the solution by adopting a mathematical statistical method according to a particle size distribution analysis result, and analyzing the influence of the centrifugal rotation rate on particle aggregation and particle size distribution; the carbon powder particle distribution in the carbon fiber centrifugation process is analyzed and monitored in real time, the centrifugation process parameters are dynamically regulated and controlled according to the real-time analysis result, and the online monitored data are used for optimizing the carbon fiber recycling process.

The invention relates to a preparation method of a tricholoma matsutake fermentation product with anti-wrinkle and firming effects as well as a product and application of the tricholoma matsutake fermentation product. The preparation method comprises the following steps: mixing a composite plant extract, a carbon source, inorganic salt and water, then sterilizing to obtain a sterilized culture medium, inoculating a composite probiotic agent into the medium, and carrying out fermentation culture to obtain the tricholoma matsutake fermentation product with anti-wrinkle and firming effects. Centrifuging, filtering and sterilizing the culture product to obtain a tricholoma matsutake fermentation product; the bacterial strains in the compound probiotic agent comprise a combination of streptococcus thermophilus, a lactobacillus paracasei YK-L003 bacterial strain and a saccharomyces cerevisiae YK-S001 bacterial strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of the three strains has an excellent skin conditioning effect, can effectively improve the skin state and resist and weaken wrinkles, and has an excellent effect when being applied to moisturizing and anti-wrinkle cosmetics.

The embodiment of the invention discloses a method for extracting extracellular vesicles from a traditional Chinese medicine dry product. The method comprises the following steps: taking a dry traditional Chinese medicine product as a raw material, and sequentially performing crushing, soaking, first-order enzymolysis, wall breaking, differential centrifugation, second-order enzymolysis, high-speed centrifugation, ultracentrifugation and ultrafiltration enrichment to obtain the extracellular vesicles. A two-step enzymolysis method is adopted in the enzymolysis process, impurities are removed to the maximum extent in a sequence difference enzymolysis mode, and the structure and function stability of the exosome are protected. According to the invention, the differential centrifugation and the enzymolysis method are alternately used, so that impurities with different sizes and different properties can be removed more thoroughly. According to the method, the cost is strictly controlled, the exosome purification efficiency is relatively high, the concentration of the obtained dry exosome is relatively high, and the operability is extremely high.

The invention belongs to the technical field of oil-gas-water separation equipment, and discloses an ultrasonic detonation rotational flow microbubble flotation device which comprises a vertical container tank with inner and outer stepped double-barrel design and a separation inner barrel, an oil gathering umbrella is arranged on the upper portion of the interior, an oil discharge connector is arranged on the outer wall of one side, and surrounding physical demulsification coalescence filler is arranged on the upper portion of the area between the tank and the inner barrel. And the lower part of the separation inner cylinder is provided with a reverse symmetrical double-tangential rotational flow inlet, the bottom of the separation inner cylinder is externally provided with a purified backflow water inlet and is communicated with the purified water diversion outlet in a process manner, and the bottom of the separation inner cylinder is internally provided with a gas-water mixer and an ultrasonic knocker. By combining ultrasonic cavitation, rotational flow centrifugation, micro-nano bubbles and physical coalescence demulsification technologies, the diameter of the bubbles is reduced by 15 microns or below through the ultrasonic detonation cavitation effect, fine oil drops and suspended particles which are difficult to treat through traditional flotation and are 10 microns or below can be captured, the method is suitable for multi-phase separation of discrete high-concentration fine pollutants, and the flotation efficiency is improved. Complex separation scenes such as oily sewage, micro-plastics and mineral flotation can be treated.

The invention discloses a precise control method and system for centrifugal machine parameters of a tubular pile centrifugal process, and relates to the technical field of tubular pile production processes. Comprising the following steps that centrifugal machine parameters in historical production and strength data of produced pipe piles are obtained, the centrifugal machine parameters comprise the centrifugal machine rotating speed, the centrifugal time and the vibration frequency parameter, and a training sample set is generated; establishing a tubular pile strength prediction model, taking the data in the training sample set as input, and taking the tubular pile strength as a label to train the prediction model; real-time centrifugal machine parameters are input into the model, if the production predicted strength data are larger than or equal to the required strength, the current centrifugal machine parameters are kept, and if the production predicted strength data are smaller than the required strength, the centrifugal machine parameters are corrected based on the compactness and the resonance frequency of the pipe pile, and corrected parameters of the centrifugal machine are obtained; and outputting correction parameters of the centrifugal machine to accurately control the centrifugal machine.

The invention relates to the technical field of daily chemicals, in particular to a preparation method and application of a sapindus mukorossi fermentation product with oil-controlling and dandruff-removing effects. The preparation method comprises the following steps: (1) drying soapberry in the air, separately powdering peel and seeds, adding 75-80 parts of water, performing ultrasonic extraction at 35 DEG C for 2 hours, then adding 0.5-0.9 part of dipotassium phosphate, sterilizing, and inoculating 3-5% of lactic acid bacteria liquid for fermentation; (2) after the fermentation is finished, adding 3-7 parts of sterilized aloe extract, 2-5 parts of mulberry leaf extract, 5-10 parts of tea bran extract and 6-9 parts of rose extract, adjusting the pH value to 5-7, inoculating 3-5% of schizosaccharomyces pombe, culturing for 24-36 hours at 28-30 DEG C, and introducing oxygen at 0.3-0.5 L / min; and (3) centrifuging at 10000r, and filtering through a sieve plate to obtain clear fermentation liquor, namely the sapindus mukorossi fermented product. The soapberry peel and the soapberry seeds are separately crushed, and the peel powder and the seed powder are mixed according to a ratio of 90%: 10% for use, so that the saponin content and the irritation are favorably controlled, and the use feeling is improved.

The invention belongs to the technical field of air compressor filters, and particularly relates to a compressor front air inlet oil removal filter which comprises a shell, an upper end cover and a lower end cover are fixedly connected to the upper end and the lower end of the shell respectively, a through medium inlet and a through medium outlet are formed in the two sides of the shell respectively, and a partition plate is fixedly connected into the shell; two pressing rings are rotationally connected to the partition plate, an impeller is fixedly connected to the bottom of each pressing ring, a filter cylinder is fixedly connected into each impeller, the impellers rotate at a high speed to conduct centrifugation so that airflow can flow outwards from the interiors of the filter cylinders, a driving part is arranged below each impeller, and an oil removing and cleaning part is arranged in each filter cylinder. A back flushing cleaning mode that the impeller and the filter cartridge synchronously rotate at a high speed is adopted, the static pressure is higher, the effect is more continuous, meanwhile, air pressure distribution at all positions in the filter cartridge is more uniform, airflow can fully penetrate through the filter cartridge, and more comprehensive and efficient back flushing cleaning work is achieved.

The invention belongs to the field of health-care tea preparation, and particularly relates to begonia flower and leaf tea and a preparation method thereof. Triticale bran pulp is mixed with smashed begonia leaves, the mixture is fermented through a flavor improving fermentation inoculant and then mixed with an anti-oxidation and excitation-inhibiting solution, and the prepared low-caffeine tea base material does not cause insomnia, has the characteristics of inhibiting inflammation and resisting oxidation, protects nerve cells from being damaged, and is suitable for being used as a health-care tea base material. The honeysuckle, the endive and the soybeans are ground into thick liquid, then mixed with the repair juice, centrifuged to remove precipitates, and then frozen nano spray drying is carried out, so that the obtained nano particles for protecting the liver and the kidney and promoting circulation are higher in stability and can accelerate absorption of small intestines; a taste regulator obtained by mixing grain mucus with tartaric acid, ethyl maltol, methyl anthranilate and sorbic acid is sprayed on the surface of a begonia flower and leaf tea mixture, and the prepared begonia flower and leaf tea does not affect sleep after being drunk, does not damage livers and kidneys in organisms, is good in taste and is easy to drink for a long time.

The invention relates to the technical field of intelligent control, and discloses a centrifugal machine and a control system thereof, and the system comprises an acquisition unit which acquires feeding data to determine an inner centrifugal speed and an inner and outer centrifugal speed difference; the judging unit is used for collecting solid discharging data within preset time and judging whether the centrifugal effect of the shot blasting medium is qualified or not according to the solid discharging data; if not, waste liquid discharge data and the surface pressure of the spiral blade are collected, and whether high-pressure gas is opened or not is judged; when it is judged that centrifugation is unqualified, the processing unit collects temperature data and vibration data in the centrifugal machine to determine real-time characteristic factors, the real-time characteristic factors are compared with historical adjustment data to determine adjustment coefficients, and the inner centrifugal speed is adjusted; the correction unit collects second solid discharging data running at the adjusted inner centrifugal speed and judges whether the adjustment coefficient is corrected or not; the storage unit stores the real-time feature factors and the adjustment coefficients. The stability, the separation effect and the adaptability of the centrifugal machine are improved, and the requirement for manual intervention is lowered.

The invention relates to the technical field of nano materials, in particular to a boron nitride nanosheet, a preparation method and application of the boron nitride nanosheet in a thermal interface material. According to the preparation method, the three-roller grinding machine is adopted to replace ball milling in the prior art, introduction of ball milling beads is avoided, and the functionalization degree of the boron nitride nanosheets is remarkably improved through double-mode treatment including a gap mode and a force mode of the three-roller grinding machine; the heat conductivity and the energy storage modulus are further improved when the boron nitride nanosheet serving as a filler is filled in the thermal interface material. In addition, the method does not need additional centrifugal operation, and the yield of the boron nitride nanosheet can be close to 100%.

The invention relates to an application of sea cucumber intestine polysaccharide in preparation of uric acid lowering drugs. The preparation method of the sea cucumber intestine polysaccharide comprises the following steps: performing enzymolysis treatment on sea cucumber intestines by using papain, performing enzyme deactivation, performing centrifugation, and taking supernate; and carrying out graded alcohol precipitation on the supernate, centrifuging, taking precipitate, deproteinizing and freeze-drying. The preparation method is simple, green and environment-friendly. The sea cucumber intestine polysaccharide prepared by the method can effectively inhibit the activity of xanthine oxidase; the sea cucumber intestine polysaccharide has no toxic effect on HK-2 cells. The compound has a uric acid reducing effect on HK-2 hyperuricemia cells.

The invention relates to the technical field of plant exosome-like extracts, in particular to a liquorice exosome-like extract as well as extraction and application thereof. The method comprises the following steps: firstly, removing impurities such as cells, cell debris, organelles, large vesicles and the like in homogenate by adopting a combination mode of low-speed centrifugation and medium-speed centrifugation, and further purifying the homogenate by adopting high-speed centrifugation after the low-speed centrifugation and the medium-speed centrifugation are completed, so as to obtain the exosome-like extract. And the setting of the increasing rotation speed is beneficial to finer separation of exosome-like particles with different sizes and densities, so that the purity and quality of the extract are improved. According to the method, all centrifugal operations are carried out in a low-temperature environment of 4 DEG C; the low-temperature condition is beneficial to maintaining the biological activity and stability of the sample, and the problems of protein denaturation, enzyme inactivation and the like caused by temperature rise are reduced, so that the quality and performance of the exosome-like extract are ensured.

The invention relates to an iron-based phosphate sodium ion positive electrode material and a preparation method and application thereof, and the preparation method comprises the following steps: respectively dissolving an iron source containing a + 2 valence state and a + 3 valence state and a sodium-containing phosphorus source in deionized water, uniformly mixing, reacting, centrifuging and drying to obtain a powder precursor; the preparation method comprises the following steps: mechanically mixing a precursor, a sodium source, a carbon source, a doping agent and a solvent by adopting ball milling or sand milling, then separating out solid particles by utilizing centrifugation, and carrying out vacuum drying on the obtained solid; and calcining and sintering in an inert atmosphere or a hydrogen-argon mixed atmosphere, and naturally cooling to room temperature to obtain the iron-based phosphate sodium ion positive electrode material. Compared with the prior art, the method has the advantages that the raw material utilization rate is high, the reaction ignition loss rate can be as low as 3.16%, meanwhile, reactants in the method are easy to obtain, the synthesis process is environment-friendly, safe and low in cost, the product impurity content is low and stable, and the obtained material shows excellent electrochemical performance when applied to the sodium ion battery and can be used for industrial production.

The invention belongs to the technical field of shale oil development and research, and discloses a shale oil mobility evaluation method based on a two-dimensional nuclear magnetic resonance experiment, which comprises the following steps: dividing an oil-containing shale sample harvested by a closed coring well into three parts which are respectively marked as a sample 1, a sample 2 and a sample 3; carrying out a TOC determination experiment on the sample 1 to obtain the TOC content of the sample; carrying out a step-by-step pyrolysis experiment on the sample 2 to obtain the contents of the sample S1-1 and the sample S2-2; calculating to obtain a shale oil mobility evaluation factor which is marked as FMO; carrying out a two-dimensional nuclear magnetic resonance experiment on the sample 3 before and after centrifugation to obtain the movable oil content of the sample before and after centrifugation, calculating to obtain the percentage of two-nuclear magnetic resonance movable oil, and recording the percentage as MO2D-NMR; and shale oil mobility evaluation is realized through analysis of the relationship between MO2D-NMR and FMO. According to the method provided by the invention, nondestructive testing of the sample can be realized in the two-dimensional nuclear magnetic resonance experiment process, the loss of light hydrocarbon can be reduced to the greatest extent, and the mobility evaluation precision of shale oil is effectively improved.

The invention provides a calibration method for a nuclear magnetic fluid signal conversion relation with shale as a carrier and application, and belongs to the technical field of unconventional oil-gas exploration and development. The method comprises the steps that shale samples are pretreated, and fluid saturation is conducted on the shale samples; the saturated shale is subjected to centrifugation and drying treatment, and T2 spectrogram testing is carried out by adopting nuclear magnetic resonance equipment in different treatment stages; and the conversion relation of the oil-water fluid in the shale is established by comparing the attenuation amount of the fluid in the shale pores at different stages with the T2 spectrogram signal difference value. The mass change caused by fluid loss in the closed coring shale is used as the verification basis of the conversion relation, and the reliability of the method is ensured. And then crude oil with different maturity degrees and formation water fluids with different mineralization degrees are adopted to repeat the operation, the calibration conversion relation of the crude oil with different maturity degrees and the formation water with different mineralization degrees is obtained, then the change rule of the calibration conversion relation is disclosed, and the accuracy and scientificity of the nuclear magnetic resonance technology in the aspect of shale fluid evaluation are improved.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention discloses a method for screening and culturing active plastic degrading bacteria based on combination of Raman spectrum and heavy water labeling, which realizes rapid screening of high-activity plastic degrading bacteria within 7 days by utilizing intestinal microorganisms under the condition of inorganic salt culture incubation with plastic as a unique carbon source. The method comprises the following steps: dissecting and homogenizing intestinal tracts of tenebrio molitor fed with plastics under a sterile condition, harvesting enteric microorganisms through iohexol density gradient centrifugation, incubating the enteric microorganisms in an inorganic salt culture solution containing heavy water and plastics to serve as a treatment group, centrifugally cleaning an incubated to-be-detected substance, and performing Raman detection, the high-activity plastic degrading bacteria can be sorted for further downstream sequencing and culture. The method is rapid, efficient, easy and convenient to operate and suitable for rapidly screening, identifying and culturing the high-activity plastic degrading bacteria from a complex natural environment, and development of plastic biodegradation and harmless treatment processes is facilitated.

The invention discloses an antler bone strengthening peptide for promoting bone repair as well as a screening method and application thereof, and belongs to the technical field of traditional Chinese medicinal material polypeptides. And the antler bone strengthening peptide is one of G40, G44 and G45. Grinding and crushing the antler, homogenizing the lysate, performing ultrasonic centrifugation, precipitating protein with methanol, centrifuging and discarding the supernatant; extracting, desalting, and freeze-drying to obtain a antler polypeptide extract; liquid chromatography-mass spectrometry detection, Peaks 8 search software and De Novo method analysis are adopted, according to the conditions that ALC is larger than or equal to 95%, local context is larger than or equal to 95%, Area is larger than or equal to 1000000 and an NCBI database, free polypeptide is obtained through comparison, the biological activity probability, the peptide fragment toxicity and the sensitization of the peptide fragment are predicted, and candidate polypeptide is obtained; synthesizing candidate polypeptides by a solid-phase synthesis method; the influence of the candidate polypeptide on osteoblast proliferation is evaluated, and the antler bone strengthening peptide is screened out. The antler bone strengthening peptide promotes osteoblast proliferation, and reveals a potential bone repair mechanism of the antler bone strengthening peptide.

The invention discloses a method for extracting curcumin from turmeric, which comprises the following steps: adding a deep eutectic solvent solution into turmeric powder, adding pectinase, uniformly mixing, adding terpilenol, performing magnetic stirring extraction and centrifugation, and taking an upper solution, thereby obtaining the curcumin. According to the method, a solvent system combining a deep eutectic solvent (DES), pectinase and terpilenol is adopted, extraction and enrichment of curcumin in turmeric can be achieved through a simple one-step method, the process is greatly simplified, recycling and reutilization of reagents are achieved, and compared with an existing extraction method, the extraction method has the advantages of being easy to operate, green, environmentally friendly, high in yield and the like. The method is short in extraction time, low in production cost and suitable for large-scale industrial production.

The invention relates to the technical field of biological medicine, in particular to a hepatitis A inactivated vaccine and a preparation method thereof. Comprising the following steps: cell amplification, virus culture, virus harvesting, cell disruption, ultrafiltration concentration, inactivation, purification and finished product obtaining. The virus culture comprises the following steps: separating from excrement of a hepatitis A patient and carrying out adaptive passage to obtain a virus strain; adding a carrier into a bioreactor, sterilizing for later use, inoculating a virus strain into a collected cell suspension, and transferring into the bioreactor for culturing; the cell disruption step comprises the following steps: carrying out ultrasonic oscillation on a collected virus harvesting liquid to obtain a cell disruption liquid; trichloromethane is added for extraction and centrifugation, and supernatant virus liquid is collected; the invention provides a preparation method of a hepatitis A inactivated vaccine, which is used for preparing the efficient, safe and stable hepatitis A inactivated vaccine by optimizing virus culture and inactivation processes. The method has the advantages of high virus yield and low production cost, and is suitable for large-scale production and application.

The preparation method comprises the following steps: adding tetraethyl silicate and porous carbon into deionized water, uniformly stirring, carrying out ultrasonic treatment, standing at room temperature for 6 hours, removing supernate, pre-drying precipitate, heating to 300 DEG C, keeping for 2 hours, putting into a tubular furnace, and carrying out heat preservation for 2 hours under the protection of N2 atmosphere, so as to obtain the silicon-doped porous carbon-loaded potassium monatomic solid hydrogen storage material. Heating at 900 DEG C for 3 hours to obtain a silicon-doped porous carbon material; and dissolving potassium chloride and the silicon-doped porous carbon material in deionized water, stirring at room temperature for 1 hour, standing and precipitating for 4 hours, then carrying out high-speed centrifugal treatment, removing supernate, retaining precipitates, and carrying out heat treatment at 300 DEG C for 3 hours to obtain the hydrogen storage material. The silicon-doped porous carbon material prepared by the invention shows an ultrahigh specific surface area, and provides a large number of active sites for hydrogen adsorption, so that the hydrogen storage capacity of the material reaches 5.2 wt%.

The present invention belongs to the technical field of bioreactors, and in particular relates to a high-density culture method for porcine myoblasts based on a bioreactor. Through the steps of resuscitating porcine myoblasts, cell-microcarrier attachment treatment, bioreactor stirring mode conversion, counting and centrifugal cell collection, the porcine myoblasts are difficult to reach a high density in a bioreactor, and a harvest density of 10^6 cells / mL is achieved in the bioreactor, meeting the requirements of cell culture meat production on cell yield; at the same time, the culture time is shortened by more than 20%, greatly saving energy consumption cost and time cost.

The invention relates to the technical field of water treatment, and provides an efficient chitosan-polyferric sulfate composite coagulant and a preparation method thereof. In order to solve the problems that a traditional inorganic coagulant is prone to secondary pollution, an organic coagulant is high in cost and complex in process control and the like, the invention provides a solution that natural chitosan serves as a raw material, the charge characteristic of the natural chitosan is enhanced through quaternary ammonium salt modification, and then the natural chitosan is compounded with the inorganic coagulant according to a specific proportion. The preparation method comprises the following steps: dissolving chitosan in a 2% acetic acid solution, adjusting the pH value to weak acidity, introducing quaternary ammonium salt, carrying out catalysis by an initiator at a constant temperature of 70 DEG C to complete a reaction, and carrying out centrifugal washing and vacuum drying to obtain modified chitosan; and then compounding with an inorganic coagulant according to a mass ratio of 1: (3-5) to form a composite system with charge neutralization and adsorption bridging functions. According to the invention, by accurately controlling the ratio of the components and process parameters, the preparation process is simple and controllable, and the obtained composite coagulant has the characteristics of high treatment efficiency, environmental friendliness and moderate cost.

The invention discloses injectable hydrogel for photo-thermal adjuvant therapy of periodontitis and a preparation method, and belongs to the technical field of high polymer material preparation, the preparation method comprises the following steps: reacting MXene dispersion liquid with epigallocatechin gallate and MgSO4; then sequentially carrying out ultrasonic treatment, centrifugation, washing and freeze-drying to obtain a multifunctional nanosheet MP-Mg; adding the multifunctional nanosheet MP-Mg into an oxidized gellan gum aqueous solution, and carrying out a mixed reaction to obtain a solution A; mixing the quaternary ammonium salt chitosan aqueous solution and the carboxymethyl chitosan aqueous solution in equal volume, and reacting to obtain a solution B; and mixing the solution A and the solution B in equal volume for reaction. The hydrogel prepared by the preparation method disclosed by the invention has injectability, photo-thermal responsiveness, biological membrane penetrability, synergistic antibacterial property and long-acting anti-inflammatory property, and an innovative and practical photo-thermal adjuvant therapy platform is provided for clinical treatment of periodontitis.