3167results about "Chemical analysis using titration" patented technology

Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

The invention provides an integrated sample-processing system and components thereof for preparing and / or analyzing samples. The components may include a transport module, a fluidics module, and an analysis module, among others.

The invention is directed to a device and a method for carrying out experiments of chemical, biochemical, biological and physical analysis, reaction and assay employing a reaction unit, in a preferred embodiment comprising: a capillarity reaction chamber, non-capillarity zone and bottom structure, which can use capillary action to quantitatively take up by itself and hold liquid in the reaction chamber. A multi-unit plate, which contains a number of reaction units on a plate frame, can be used for parallel experiments in conjunction with reservoir plate, waster pad, and liquid transfer guider.

The invention provides a method for monitoring hydrology and water quality of a river basin under influence of water projects based on Internet of Things. According to the method, various fixed and flowing sensors are additionally mounted so as to obtain real-time hydrology and water quality data of important water areas, in combination with a video technology, monitoring on a spot environment is achieved, the monitoring information is transmitted through the Internet of Things, intelligent prediction for hydrology and water quality of the river basin is carried out through a neural network, and finally, critical applications, such as current situation assessment, tendency estimation, implementation effect estimation and extreme event handling, are achieved through an expert system. The method provided by the invention can provide real-time, reliable and complete hydrology and water quality information for the river basin under influence of water projects, and can achieve cross-regional and multi-machine type integrated remote protection for the river basin.

Methods and devices for reducing evaporation of sample materials from the wells of multiwell plates are disclosed which find particular utility when the plates are placed in a stacked configuration. An example of the methods includes providing at least a first multiwell plate which is configured to be placed in a stacked configuration with at least one second multiwell plate, the at least first multiwell plate having a plurality of wells for receiving sample material therein and opposing side walls which extend around the plate and which define a ridge spaced inwardly of the side walls and extending around the plate between the side walls and the plurality of wells, the method including at least partially filling the ridge with a liquid such as water or buffer solution. The ridge can include one or more ribs which extend upwardly from a lower surface of the ridge to help reduce sloshing of the liquid contained within the ridge and to add structural strength and rigidity to the multiwell plate. The second multiwell plate can be provided with a downwardly extending flange which extends around a lower surface of the plate and which is configured to be removably received by the ridge of the at least first multiwell plate such that when the second multiwell plate is removably positioned on top of the at least first multiwell plate in a stacked configuration, the flange extends at least partially into the ridge and contacts the liquid to thereby create a substantial evaporation barrier to minimize evaporation of sample liquids in the wells of the at least first multiwell plate.

A constant pathlength multi-assay plate cover for multi-assay plates, comprising, a flat top side and a flat bottom side, the bottom side having solid cylindrical projections of equal length extending downwardly from the flat bottom side, wherein each cylindrical projection is centered about the optical axis passing through a corresponding sample well of a multi-assay plate, thereby eliminating the meniscus and evaporation effects.

In a preferred embodiment, a method of performing biological assays, including: providing a longitudinally extending carrier tape having thermally formed therein a plurality of reagent receiving wells; adding a reagent to each of said reagent receiving wells; permitting each of said reagent receiving wells to incubate at a predetermined temperature for a predetermined time; and performing a biological analysis on each of said reagent receiving wells.

Disclosed herein is a modular test tube rack. The rack contains multiple sub-racks that can be coupled together to form the test tube rack. The sub-rack can be designed to fit into a variety of scientific instrumentation including a fixed rotor centrifuge. The assembled test tube rack can be of a format and size that allows use of standard array pipetters. Thus, a system is provided allowing use of standard array pipetters and high g centrifugation.

A pH-buffer measurement system that has at least one source for modifying the pH of a fluid entering the system, the source selected from an acid source and a base source. The acid source adds an acid equivalent to provide an acid reacted fluid and the base source adds a base equivalent to provide a base reacted fluid. The source is in fluid communication with a flow path and a component for determining a fluid characteristic of the acid reacted fluid or the base reacted fluid. The fluid characteristic that is measured is any one of a gas phase pressure, an electrical conductivity, or thermal conductivity.

A microarray assay system using an array-of-arrays plate mounted on the top surface of a vacuum fixture, and an automatic handling system that handles the plate and fixture assembly. The A2 plate includes a tray formed of a flexible material and a rigid frame for mounting on the fixture. The fixture has a plurality of orifices opening at the top surface and connected to a vacuum source to create a negative pressure to hold the flexible plate against the top surface of the fixture. The automatic handling systems that can be used with the plate and fixture assembly include a microarray printing machine, liquid handling robot, hybridization and incubation devices, and microarray imaging devices.

An apparatus for separating magnetic particles from a liquid which contains said particles, said liquid being contained in an elongated vessel (11) having a length axis (12), said vessel being arranged in a vessel holder (13) with its length axis (12) in a substantially vertical position, said vessel (11) having a bottom and a tapered cross-section that diminishes towards the bottom of the vessel and a side wall (14) which has an outer surface which forms an angle with the length axis (12) of said vessel (11). This apparatus comprises a magnet (15) having a plane outer surface (16) and being adapted for being moved by transport means (17) along a motion path (25), said magnet (15) and said transport means (17) being so arranged with respect to said vessel (11) that over a portion of said motion path (25) said plane outer surface (16) of said magnet is in contact with a portion of said outer surface of said side wall (14) of said vessel (11), and transport means (17) for moving said magnet (15) between a first predetermined position and a second predetermined position along said motion path (25).

A highly automated, high volume multichannel pipetting system which transfers liquid from mother plates to daughter plates, or from a fill station to daughter plates. The mother plates are stacked in one set of stacker assemblies, while the empty daughter plates are stacked in another stacker assembly. A plate handling assembly which is capable of moving the plates in three orthogonal directions retrieves the plates from the stacker assemblies, carries them to the pipetting head, and returns them to other stacker assemblies. The pipetting head is removable for replacement or repair thereof, or for insertion of another head assembly having a different number of pipetting channels. The head slides into the housing on slideways, and is retained in place by manually operable, threaded knobs mounted on shafts. The stacker assemblies include a chimney which is removable from a base. The plates may be stacked in the chimney and then inserted on the base. The base includes solenoids whose arms are retractable to permit plates to be retrieved or replaced one at a time. The chimneys contain flaps which serve to retain the plates within the chimney if manually removed from the base. Doors in the stacker chimneys allow manual insertion and replacement of plates. Methods of operation of the pipetting system are also disclosed.

A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.

The present invention provides ratiometric fluorescent pH sensors for non-invasive, continuous monitoring of pH in such applications as fermentation processes. The ratiometric fluorescent pH sensors comprise a fluorescent dye that exhibits a shift in excitation wavelength with a corresponding shift in pH in the local environment of said fluorescent dye. Ratiometric measurements of the emission intensities at dual excitation maxima correlate to pH. Also provided is a fluorescent dye 6-methacryloyl-8-hydroxy-1,3-pyrene disulfonic acid (MA-HPDS). Further provided are systems and methods to non-invasively and continuously monitor pH.

Rapid spectrum assay of multiple samples with infrared light is made possible by devices and methods that increase total light throughput. Multiple wavelength scan with Fourier analysis is combined with large numbers of sample wells located within infrared light compatible solid materials. In particular, very large scale measurement devices and systems for their use are fabricated from lithography and other techniques used for semiconductor processing.

A multi-modality fluorescence reference plate comprising wells coated with a fluorogenic compound is described, together with a method of producing such a plate. The plate has utility for calibrating fluorescent plate readers and imaging systems for measuring steady-state fluorescence, time-resolved fluorescence, fluorescence lifetime and / or fluorescence polarisation.

A device (1) for aspirating and dispensing liquid samples having a pump (2), which comprises a cylindrical chamber (3), a piston (4) movable in this cylindrical chamber, and a piston drive (5) engaging on the piston. The device additionally comprises a pulse generator (6), which effects dispensing of samples from a liquid by generating pressure waves in this liquid and a tip (8) connected via a line (7) with the cylindrical chamber (3), with the piston drive (5) comprising a first drive (9) and a second drive (10), implemented as a pulse generator (6). Such a device comprises a channel (27) for rinsing or flushing the cylindrical chamber (3), with the channel (27) discharging into the cylindrical chamber (3). Furthermore, the invention also concerns systems with, for example, 384 or more such devices that are arranged in an array.

A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

An assay device (1) comprises a base (2) and glass plate lid (3). The base (2) has an array of shallow microwells (4), each having a flat rim (9), all rims being co-planar. When the lid (3) is placed on the base (2) a thin capillary gap (10) is formed on each rim, acting as a liquid seal for a microwell chamber. The liquid is excess sample liquid and further excess is accommodated in overspill cavities (6) between the microwells (4). Because of the liquid seal and shallow configuration the benefits of microfluidic devices are achieved together with the handling convenience and use of conventional detection equipment of conventional microplate devices.

Microfluidic devices fabricated from paper that has been covalently modified to increase its hydrophobicity, as well as methods of making and using thereof are provided herein. The devices are typically small, portable, flexible, and both easy and inexpensive to fabricate. Microfluidic devices contain a network of microfluidic components, including microfluidic channels, microfluidic chambers, microwells, or combinations thereof, designed to carry, store, mix, react, and / or analyze liquid samples. The microfluidic channels may be open channels, closed channels, or combinations thereof. The microfluidic devices may be used to detect and / or quantify an analyte, such as a small molecules, proteins, lipids polysaccharides, nucleic acids, prokaryotic cells, eukaryotic cells, particles, viruses, metal ions, and combinations thereof.

A cell culture apparatus consists of a vessel or array of vessels, each comprising a substantially flat bottom and substantially vertical sides, and composed of a substantially gas permeable material. The inner bottom surface of the vessel is commonly textured to provide the cells with adequate features for attachment and spreading. The vessel may include an integral annular flange which can be used to suspend the vessel from a suspensory element of a rack structure. To promote cell attachment and growth, the inner surfaces of the vessel bottom and sides may be coated with commonly available bio-active materials. Cells cultured in the system commonly grow in three dimensions for extended periods of time, and often produce significantly higher quantities of cellular products than cells grown in conventional labware.

Provided is a multiwell plate for an easy liquid removal, wherein the mutiwell plate includes a well with at least one pore in the sidewall defining the well, and a method of analyzing a target material using the multiwell plate.

A fluidic assay device or test format that can regulate or control the sample flow rate and modulate the manifestation of test results to reduce or eliminate errors is described. The assay device has a substrate with a flow-rate control zone that regulates the amount of time needed for development and appearance of a visual signal in the observation-feedback zone until the color transition in the detection zone reaches color stability. The present invention also describes absorbent articles incorporating such an assay device and methods of monitoring dehydration or testing ion strength of a urine sample using such a test format.

Apparatus and methods for aspirating and dispensing reagents are provided. Aspirating of reagents is accomplished by a probe, a vial insert and reagent vial according to the present invention wherein a seal is formed between the probe and the vial insert when the probe engages the vial insert. Dispensing of reagents is accomplished by a probe and a probe dispense and wash station according to the present invention wherein a seal is formed between the probe and the vial insert when the probe engages the probe dispense and wash station.

Devices, methods and systems effective to evaluate a physical or chemical property of a surfactant-treated biological fluid sample are provided.

A microwell including a plurality of MRI-readable indicia. The indicia indicate the identification or location of at least one predefined well or a plurality of wells. The indicia are selected from a mark, figure, number, text, code, barcode, readable texture or indication thereof.

The present invention relates to a chemical compound that has applications as a luminescent indicator dye, and to an optical sensor, typically employed for determination of near-neutral pH values of aqueous samples. The optical sensor has particular application in the pH determination of body liquids such as, for example, blood, plasma and serum.

An assay device (1) comprises a base (2) and glass plate lid (3). The base (2) has an array of shallow microwells (4), each having a flat rim (9), all rims being co-planar. When the lid (3) is placed on the base (2) a thin capillary gap (10) is formed on each rim, acting as a liquid seal for a microwell chamber. The liquid is excess sample liquid and further excess is accommodated in overspill cavities (6) between the microwells (4). Because of the liquid seal and shallow configuration the benefits of microfluidic devices are achieved together with the handling convenience and use of conventional detection equipment of conventional microplate devices.