5 results about "Prokaryotic cells" patented technology

Provided is a self-selection DNA editing system. A subject self-selection DNA editing system includes: (a) a gene editing tool, and (b) a donor DNA comprising a bacteriocin unit, which includes a nucleotide sequence encoding a bacteriocin protein and / or a nucleotide sequence encoding a bacteriocin immunity protein. In some cases, (a) and (b) are present on the same nucleic acid (e.g., plasmid DNA). When a bacteriocin unit of the subject disclosure is present on (as part of) a donor DNA, the bacteriocin unit is configured for integration into a target DNA such as a genomic DNA. The present disclosure also provides methods of modifying a microbial community and methods of editing a prokaryotic cell to facilitate selection. The targeted prokaryotic cell(s) can be in culture or can be in situ (e.g., part of a microbial community).

The present invention addresses the problem of, in a method for extracting a target protein from prokaryotic cells expressing the target protein, preventing the contamination of the target protein with foreign substances and increasing the recovery rate of the target protein.　The method for extracting a target protein from prokaryotic cells that is disclosed in the present description is characterized by comprising: a step for mixing a culture solution containing prokaryotic cells that express the target protein with a carboxylic acid at a final concentration of 0.5-15% (v / v) inclusive to prepare a mixed liquid; and a step for extracting the target protein from the prokaryotic cells in the mixed liquid.

The present invention provides a method of preparing eukaryotic cellular material from a mucosal sample, which comprises a mixture of mammalian and prokaryotic cells. The method comprises the steps of: (i) obtaining the mucosal sample; (ii) preferentially lysing the mammalian cells in the mixture into a soluble state; (iii) separating the lysed soluble cellular material from non-lysed prokaryotic material; and (iv) removing any prokaryotic material in the lysed solution cellular material. The method yields a sample of mammalian cellular material that is substantially free of prokaryotic cells and / or cellular material. Also provided are methods of diagnosing a disease or disorder and treating such diseases / disorders, comprising performing the preparation method described herein and analysing the sample of mammalian cellular material for a marker associated with the disease or disorder.