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8 results about "Transfer DNA" patented technology
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The transfer DNA (abbreviated T-DNA) is the transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes(actually an Ri plasmid). The T-DNA is transferred from bacterium into the host plant's nuclear DNA genome. The capability of this specialized tumor-inducing (Ti) plasmid is attributed to two essential regions required for DNA transfer to the host cell. As the T-DNA is bordered by 25-base-pair repeats on each end. Transfer is initiated at the right border and terminated at the left border and requires the vir genes of the Ti plasmid.
Plant transformation
PCT designated stage expiredWO2025111662A1Plant peptidesVector-based foreign material introductionBiotechnologyTransgene
The present invention relates to transfer DNAs which can be used to produce a genetically modified plant that only has DNA from that species of plant. The present invention also relates to genetically modified plants with a transfer DNA of the invention which has been processed during integration and methods of making such plants. Also provided is a DNA construct and methods of making a multigene construct comprising five genes which confer upon a plant enhanced resistance to a wide variety of fungal pathogens.
Owner:COMMONWEALTH SCI & IND RES ORG
Targeted genome modification using circular single-stranded DNA
The present invention is directed to methods for generating one or more genetically modified cells by using a circular single stranded DNA (CiSSD) as a donor template and targeting genome modification. These methods include transferring one or more DNA polynucleotides into the cell for site-specific nuclease-mediated DNA repair and selecting one or more cells having the transferred DNA incorporated into the cell's genome.
Owner:FULL CIRCLES THERAPEUTICS
Targeted modification of genomes using single stranded circular DNA
The present invention relates to methods of generating one or more transgenic cells using circular single stranded DNA (CiSSD) as a donor template, as well as target genome modification. The methods include transferring one or more DNA polynucleotides into a cell for site-specific nuclease-mediated DNA repair, and selecting one or more cells into which the transferred DNA is introduced in the genome of the cell.
Owner:FUSHENGYUAN CO LTD
Methods for maintaining methylation in amplification of methylated nucleic acid molecules
The present disclosure provides methods for preparing preparations of DNA from a subject useful for determining a methylation status of a genomic region of interest. The methods comprise extracting DNA from a sample of a subject; copying one or more template DNA molecules from the extracted DNA to generate one or more copied DNA molecules; transferring methylation status from the one or more template DNA molecules to the one or more copied DNA molecules, thereby generating one or more methylation-transferred DNA molecules; and repeating the copying and transfer steps to generate a library of amplified DNA molecules comprising the one or more methylation-transferred DNA molecules. The method may employ isothermal extension at 60°C and strand-invasion or linked primers.
Owner:NATERA INC +1
Apple pollen transgenic method mediated by ferroferric oxide nano magnetic transformation system
The invention relates to the field of biology, and discloses an apple pollen transgenic method mediated by a ferroferric oxide nano magnetic transformation system. According to the method, plasmids with endotoxin removed form a compound (MNPs-DNA) under the action of ferroferric oxide nano magnetic particles, the DNA is transferred into apple pollen through MNPs under the action of an external magnetic field of 0.1-0.5 T, transgenic seeds are obtained through artificial pollination, and transgenic fruits are obtained. The method has the advantages that a tissue culture process is not needed, the period from transgenosis to germplasm material obtaining is shortened, receptor materials are not limited by genotypes, the source is wide, operation is convenient and fast, large-scale field operation is facilitated, and the method is expected to become a platform technology to be widely applied to transgenosis cultivation of other flowering plants.
Owner:CHINA AGRI UNIV
Multigene stack for rust resistance
PCT designated stage expiredWO2025111663A1Plant peptidesVector-based foreign material introductionBiotechnologyTransgene
The present invention relates to a DNA construct and methods of making a multigene construct comprising five genes which confer upon a plant enhanced resistance to a wide variety of fungal pathogens. Also provided are transfer DNAs which can be used to produce a genetically modified plant that only has DNA from that species of plant. The present invention also relates to genetically modified plants with a transfer DNA of the invention which has been processed during integration and methods of making such plants.
Owner:COMMONWEALTH SCI & IND RES ORG
Targeted genome modification using circular single-stranded DNA
PendingUS20260146261A1Organic active ingredientsImmunoglobulin superfamilySingle strandGene Modification
The present invention is directed to methods for generating one or more genetically modified cells by using a circular single stranded DNA (CiSSD) as a donor template and targeting genome modification. These methods include transferring one or more DNA polynucleotides into the cell for site-specific nuclease-mediated DNA repair and selecting one or more cells having the transferred DNA incorporated into the cell's genome.
Owner:FULL CIRCLES THERAPEUTICS
Antimicrobial agents and methods
The present invention relates to means for performing conjugation between bacteria, and in particular, the present invention relates to a carrier bacterium comprising an antimicrobial agent and methods of use. The carrier bacterium is capable of transferring DNA encoding the agent to a target cell by conjugation. The present invention further relates to promoting growth or feed conversion in an animal. The present invention further relates to killing Salmonella or inhibiting growth or proliferation of Salmonella.
Owner:FULIAN FOOD SCI CO LTD