639 results about "Glutamine" patented technology

The application relates to an engineering bacterium for high yield of observation blue and a construction method and application thereof, and belongs to the technical field of biological synthesis of natural dyes. The engineering bacterium for high yield of observation blue expresses icd, bpsA, glnA Y405F and gdhA; the engineering bacterium knocks out acnR, yggB, glsK, aceA and ldh. By knocking out the yggB and aceA genes, performing site-directed mutation (Y405F) on the glnA gene, replacing the glsK gene with the glnA Y405F gene, replacing the acnR gene with the icd gene, replacing the ldh gene with the gdhA gene, and integrating the bpsA gene, the application can block the formation of by-products such as lactate and succinic acid in the observation blue synthesis process, improve the flow direction of the citric acid->isocitric acid->alpha-ketoglutaric acid->glutamic acid->glutamine path, and then improve the intracellular glutamine concentration, so that the yield of observation blue is improved.

The present application relates to the technical field of biocatalysis and biosynthesis, and specifically discloses a natural blue pigment and a biosynthesis method therefor. The present application uses metabolically engineered bacteria to express indigoidine synthetase and phosphopantetheinyl transferase, to catalyze glutamine and N-acetylglutamine to biosynthesize a natural blue pigment N-acetylindigoidine, and deduces its molecular structure by means of mass spectrometry, nuclear magnetic resonance and other methods. The present application achieves catalytic synthesis of N-acetylindigoidine from glutamine and N-acetylglutamine in Escherichia coli, Corynebacterium glutamicum, Saccharomyces cerevisiae and Streptomyces. Compared with indigoidine, N-acetylindigoidine has a maximum absorption wavelength of 584 nm, possesses better color brightness, and has more stable coloration that is not easily faded, with a very broad application range and industrial production application prospects.

The invention belongs to the technical field of genetic engineering and enzyme engineering, and particularly relates to a genetically engineered bacterium for stably and efficiently producing L-isoleucine as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes E. coliTHRD as a host and contains an RNA (Ribonucleic Acid) polymerase beta-subunit mutant coding gene rpoBM; compared with a parent RNA polymerase beta-subunit, the RNA polymerase beta-subunit mutant has the advantage that the amino acid residue at the 618th site of the amino acid sequence is sequentially replaced with leucine from the N terminal to the C terminal. The genetically engineered bacterium disclosed by the invention can tolerate L-isoleucine and accumulate less alpha-ketobutyric acid, and has a very good industrial application prospect.

The present application relates to the technical field of biocatalysis and biosynthesis, and specifically discloses a blue pigment and a biosynthesis method thereof. In the present application, an indigoidine synthetase and a 4′-phosphopantetheinyl transferase are expressed by a metabolically engineered strain to catalyze the biosynthesis of the blue pigment N-acetyl-indigoidine from glutamine and N-acetylglutamine, and a molecular structure of the blue pigment is inferred by mass spectrometry, nuclear magnetic resonance spectroscopy, etc. The present application achieves the catalytic synthesis of N-acetyl-indigoidine from glutamine and N-acetylglutamine in Escherichia coli (E. coli), Corynebacterium glutamicum (C. glutamicum), Saccharomyces cerevisiae (S. cerevisiae), and Streptomyces. Compared with indigoidine, N-acetyl-indigoidine has a maximum absorption wavelength of 584 nm, and a stable color having high brightness that is not easy to fade. Thus, the blue pigment shows an extensive application range and a promising industrial production prospect.

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The invention discloses a composite sunscreen composition with excellent film-forming property and sunscreen stability as well as a preparation method and application thereof, and belongs to the technical field of daily chemicals. The composite sunscreen composition is prepared from inulin lauryl carbamate, bis (lauramide glutamine) lysine sodium, hydroxypropyl trimethyl ammonium chloride hyaluronic acid, an ammonium acryloyldimethyl taurate / VP copolymer, a sunscreen agent, basic grease, and cosmetically acceptable auxiliary materials and a solvent, the sunscreen emulsion prepared on the basis of the composition can quickly form a stable, light, thin and breathable sunscreen film on the surface of skin, solves the problems that a sunscreen agent in a traditional sunscreen product is non-uniform in agglomeration and dispersion, a formed film is rigid and airtight, the sunscreen effect is easy to lose due to sweating / friction, the skin feeling is poor and the like, and has the advantages of high sunscreen stability, excellent water resistance and good skin feeling; the method can be widely applied to preparation of skin care products with a sunscreen effect.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The invention discloses a method for extracting ginsenoside based on a supercritical extraction method, and relates to the technical field of traditional Chinese medicine extraction. The ginsenoside is extracted through a supercritical extraction method, the extraction process is convenient and efficient, the purity of the ginsenoside is high, organic solvent residues are avoided, the method is green and environmentally friendly, carbon dioxide can be recycled, and the operation cost is low; through degreasing pretreatment, fat-soluble components in ginseng powder are removed, the extraction purity of saponin is increased, the solid loading capacity of supercritical extraction is reduced, the mass transfer resistance is reduced, and the extraction efficiency is improved; the ginsenoside is purified in a post-treatment mode of resin adsorption and elution, polar impurities can be further removed, glutamine is adopted for surface functionalization of the resin, acylamino and carboxyl are introduced to the surface of the resin and can form hydrogen bonds with phenolic hydroxyl groups in the ginsenoside, functional adsorption is achieved, meanwhile, impurities are removed, and the content of the ginsenoside is increased. Reaction conditions are simple and mild, and adsorption efficiency is high.

The invention discloses multi-component inulin hydrogel as well as a preparation method and application thereof, and belongs to the technical field of nano materials and medicines.The multi-component inulin hydrogel is characterized in that a medicine L-alanyl-L-glutamine contributing to intestinal mucosa repair is entrapped in the inulin hydrogel; the multi-component inulin hydrogel has the advantages that the multi-component inulin hydrogel is prepared from the inulin and composite nanoparticles (namely mesoporous polydopamine-gallium composite nanoparticles) composed of an active oxygen scavenger polydopamine and an antibacterial agent gallium ions, the repairing capacity on intestinal barriers is further enhanced, and the prepared multi-component inulin hydrogel can be used for effectively treating inflammatory bowel diseases and non-alcoholic steatohepatitis.

The invention discloses an amino acid composition for treating alopecia and application of the amino acid composition in preparation of a medicine for treating alopecia, and belongs to the field of medicine configuration products, the amino acid composition is composed of a targeting core intervention component and an auxiliary strengthening synergistic component, the targeting core intervention component is prepared from 30-50 parts of arginine, 50-155 parts of lysine and 50-120 parts of glutamic acid, and the auxiliary strengthening synergistic component is prepared from an auxiliary strengthening synergistic component and an auxiliary strengthening synergistic component. 10 to 40 parts of cysteine and 20 to 60 parts of leucine; the auxiliary strengthening synergistic component is prepared from 15 to 45 parts of tyrosine, 15 to 40 parts of glycine, 10 to 40 parts of glutamine, 20 to 35 parts of serine, 5 to 25 parts of alanine, 2 to 20 parts of aspartic acid and 15 to 35 parts of phenylalanine; the composition can be used as an active ingredient to be prepared into different dosage forms such as a pigmentum, a spray, an ointment and a liniment, is applied to prevention of alopecia and promotion of hair growth, and has a remarkable treatment effect.

There is provided a method for diagnosing a subject with early onset muscle atrophy, said method comprising: obtaining a biosample from the subject; and assaying the biosample for one or more biomarkers, said one more biomarkers are taurine, proline, citrulline, trigonelline, thymidine, ornithine, glutamate, L-pyroglutamic acid, creatinine, adenine, nicotinamide, 2-methylhippuric acid, maltol, L-arginine, hypotaurine, L-glutamine, homogentisic acid, methylhistidine, oxoglutaric acid, xanthine, L-carnitine, succinate, or a combination thereof; and identifying the subject with early onset muscle atrophy on the basis of a deviation in the one or more of said one more biomarkers.

The invention discloses functional recombinant soybean protein isolate emulsion gel as well as a preparation method and application thereof, and belongs to the technical field of soybean protein processing. The preparation method comprises the following steps: recombining three main components, namely soybean lipophilic protein, glycinin and beta-conglycinin, in soybean protein isolate (SPI) according to a certain compounding ratio, and carrying out combined modification on the recombined SPI by virtue of modification means such as ultrasonic treatment, heat treatment, magnetic field and enzyme crosslinking; the recombined SPI emulsion is subjected to micronization treatment by adopting high-pressure microjet homogenization treatment, and then the emulsion is jointly induced to be gelatinized by utilizing acid and salt ions and transglutaminase, so that the preparation efficiency, the structural strength and the stability of the emulsion gel are improved; two polyphenols, namely epigallocatechin gallate and quercetin, are co-embedded in an interface and an oil phase of the emulsion gel, so that the bioaccessibility and antioxidant activity of the polyphenols are improved, and the emulsion gel is endowed with functionality. The prepared emulsion gel can be widely applied to processing of functional food.

The invention discloses a nitrogen-containing heterocyclic compound and application thereof. The nitrogen-containing heterocyclic compound has a structure as shown in a formula (I). The compound provided by the invention has an excellent glutamine cyclase inhibition effect, can be applied to treatment of tumors, immune diseases, neurological diseases or aging, and has a good application prospect.

The invention discloses a preparation method of highland barley prefabricated powder with a low glycemic index and application of the highland barley prefabricated powder in 3D printed biscuits. According to the method, firstly, cellulase and alpha-amylase are adopted for carrying out enzymatic modification treatment on highland barley flour, the content of anti-digestion starch (RS) is remarkably increased, the content of fast-digestion starch (RDS) is reduced, and therefore the modified highland barley flour with the predicted glycemic index (pGI) reaching the low-GI food standard (GI is smaller than or equal to 55) is prepared, and the dietary requirements of people with different metabolic requirements are met. Further, the modified highland barley powder is compounded with sodium alginate, glutamine transaminase is utilized for inducing and constructing a protein-polysaccharide gel network, the incubated and cross-linked dough is subjected to freeze drying and smashing, and the highland barley prefabricated powder with the low glycemic index is obtained.The 3D printing adaptability, the structural stability and the digestion resistance of the material are effectively enhanced through the method. The highland barley biscuit has the characteristic of low glycemic index, and is suitable for making healthy food for special crowds with diabetes and the like.

The invention discloses a probiotic chitosan microsphere and a preparation method thereof, and belongs to the technical field of probiotic embedding, and the method comprises the following steps: dissolving trehalose and L-glutamine in water, suspending lactic acid bacteria in the water, mixing with a chitosan solution to obtain a mixed solution, dropwise adding the mixed solution into a sodium tripolyphosphate solution, and stirring to obtain the probiotic chitosan microsphere. And washing insoluble substances, and performing freeze drying to obtain the probiotic chitosan microspheres. The trehalose and the L-glutamine are used as protective agents and nutrient substances of the probiotics at the same time, can synergistically improve the survival rate of the probiotics, are matched with other components, comprehensively improve the tolerance and activity of the probiotics, and are combined with a specific curing means and freeze drying, so that damage of high temperature to the probiotics can be avoided; the obtained microspheres have good probiotic protection capability, stability and appropriate release performance, can effectively improve the survival rate of the probiotics in storage and application processes, and can better play roles in improving intestinal flora and improving the health level of hosts.

The invention provides a feed premix for improving chicken quality. The fresh-keeping agent is prepared from the following components in percentage by weight: 5%-15% of a flavor enhancing component, 10%-25% of a tenderness improving component, 3%-10% of an antioxidant fresh-keeping component, 2%-3% of a function enhancing component and the balance of a carrier and a diluent, the function strengthening component is prepared from beta-alanine embedded citronella essential oil and konjac glucomannan zymolyte; the flavor enhancing component comprises a rosemary extract, a yeast culture and curcumin in a mass ratio of (2-4): (1-3): 1; the tenderness improving component comprises conjugated linoleic acid, L-carnitine modified by glutamine transaminase and betaine in a mass ratio of (3-5): (2-4): 1; the antioxidant fresh-keeping component is vitamin E and tea polyphenol in a mass ratio of (1-2): 1. Through multi-component synergism and functional targeting optimization, a three-dimensional quality improvement system with enhanced flavor, improved tenderness, oxidation resistance and fresh keeping is constructed, so that the chicken quality is comprehensively upgraded.

The invention discloses a mulberry leaf flavone microcapsule which is prepared by taking mulberry leaf flavone extracted from mulberry leaves as a core material, taking mulberry leaf residue polysaccharide re-extracted from mulberry leaf residues left after the extraction of the mulberry leaf flavone and gelatin as a wall material and adopting a complex coacervation method. Before the complex coacervation reaction, the wall material solution needs to be subjected to electric field pulse treatment by adjusting the pH value to 3-3.5, and then subjected to electric field pulse treatment by adjusting the pH value to 6-7; after the wall material and the core material are mixed, a mixed solution needs to be cooled firstly, and then glutamine transaminase is added for a complex coacervation reaction. According to the mulberry leaf flavone microcapsule disclosed by the invention, the mulberry leaf residue polysaccharide and the gelatin are used as composite wall materials to carry out microencapsulation embedding on mulberry leaf flavone, and a flavone delivery system with a slow release function is constructed, so that not only is the reutilization of mulberry leaf residue wastes realized, but also the extracted mulberry leaf residue polysaccharide can be in synergistic interaction with the gelatin; therefore, the obtained mulberry leaf flavone microcapsule is excellent in stability, bioavailability and blood fat reducing effect.

The invention belongs to the field of bioactive protein peptide materials, and provides an animal-derived composite fermented skin-tendering protein peptide and a preparation method thereof. According to the invention, bovine-derived collagen and fish-derived protein are subjected to synergistic fermentation to prepare a protein peptide core, and the outer surface of the protein peptide core is coated with an edible gelatin and chondroitin sulfate composite layer; the excellent performance that a small molecule peptide core with the molecular weight being 500-1500 Da, a stable coating layer with the coating rate being 80-99%, the glycine-proline-hydroxyproline sequence collagen peptide fragment accounting for 30-60% and the pH responsive swelling ratio being 1.5-3.0 are achieved, the technical problems that traditional protein peptide is poor in stability and low in bioavailability are solved, and the protein peptide has wide application value in the field of skin care products.

In order to improve aggregation property of a PPR protein, the A6 amino acid of the 1st PPR motif from the N-terminus (M1) is made more hydrophilic. Further, the A9 amino acid of M1 is made to be a hydrophilic amino acid or glycine. The A6 amino acid is preferably asparagine or aspartic acid, and the A9 amino acid is preferably glutamine, glutamic acid, lysine, or glycine. Proteins containing such a PPR motif as M1 motif may have not only improved aggregation property, but also high binding power to a target nucleic acid.

The invention discloses a preparation method of a stable Pickering emulsion based on enzymic method modified whey protein microgel particle-araboxylan covalent composite particle, which comprises the following steps: preparing whey protein microgel particles by cross-linking glutamine transaminase, mixing the whey protein microgel particles with an araboxylan solution, and adding an enzyme method modified whey protein microgel particle-araboxylan covalent composite particle to prepare the Pickering emulsion. Adding horse radish peroxidase and a hydrogen peroxide solution to carry out enzymatic modification to obtain a water phase, mixing the oil phase and the water phase according to a volume ratio, carrying out IKA homogenization, and then carrying out ultrasonic treatment to obtain a Pickering emulsion with stable covalent composite particles; the preparation method is simple, harmful substances cannot be generated in the preparation process, and meanwhile, the preparation method is low in production cost, short in production period, rich in nutrition, green, free of pollution and high in economic utilization value; the obtained Pickering emulsion has high viscosity and excellent storage stability and environmental stability, an effective delivery carrier construction strategy is provided for construction of a fat-soluble and heat-sensitive bioactive micromolecule delivery system, and the application range of protein microgel particles in the fields of food, medicine and the like is widened.

The invention provides a gamma delta T cell culture method and a culture medium, and belongs to the technical field of T cell culture. Modified nanoparticles, zoledronic acid, vitamin C and glutamine are added into a 1640 culture solution, the concentration of the modified nanoparticles ranges from 0.5 g / L to 1 g / L, the concentration of the zoledronic acid ranges from 0.5 mu mol / L to 1.5 mu mol / L, the concentration of the vitamin C ranges from 200 mu mol / L to 500 mu mol / L, and the concentration of the glutamine ranges from 0.1 mmol / L to 0.2 mmol / L. The prepared culture medium can efficiently stimulate amplification of the gamma delta T cells and improve the killing activity of the gamma delta T cells, meanwhile, the survival rate of the gamma delta T cells is also improved, and meanwhile, the culture method of the gamma delta T cells is simple, low in cost and wide in application prospect.

The invention provides a compound serving as a glutamine cyclase inhibitor and application of the compound, and belongs to the field of medical chemistry. The structure of the compound is shown as a formula I. The compounds have good inhibitory activity on sQC and gQC enzymes and mutants thereof, and the half inhibitory concentration of most compounds reaches the sodium mole level, so that the compounds can be used for preparing glutamine cyclase inhibitors. In addition, the compounds can also be used for preparing medicines for preventing and treating diseases related to glutamine cyclase. The invention lays a material basis for research and development of glutamine cyclase inhibitor drugs, and has a good application prospect. Formula I.

The invention belongs to the technical field of food, and provides a special enzyme composition for infant soybean milk powder and soybean milk powder thereof. A three-stage directional enzymolysis technology is adopted, the three-stage directional enzymolysis technology comprises glutamine transaminase deamidation pretreatment, bacillus subtilis protease-trypsin-papain first-stage hydrolysis and aminopeptidase-phytase-beta-glucosidase second-stage hydrolysis, and conductivity control, carbon dioxide ventilation in-situ complexation and spray drying processes are combined. The free amino nitrogen of the product is increased by more than or equal to 20%, the proportion of 200-1500Da peptide fragments is more than or equal to 70%, the median particle size is 50-180 microns, the water activity is less than or equal to 0.30, the instant dissolving time is less than or equal to 30 seconds at 25 DEG C under the water hardness of 15-200ppm, the settling rate is less than or equal to 3% after standing for 30 minutes, and the zeta potential is-10 to-25mV. The palatability contradiction between deep hydrolysis and bitterness inhibition of the bean-based infant formula powder, the brewing contradiction between high-calcium reinforcement and instant stability and the process controllability contradiction between three-stage enzymolysis and ionic strength accumulation are effectively solved, and the bean-based infant formula powder has wide application value in the fields of bean-based infant formula food and vegetable protein nutritional products.

The invention belongs to the technical field of plant tissue culture, and particularly relates to an induction culture medium for embryogenic calluses of rubber tree variety thermal reclamation 628, a method for inducing somatic embryogenesis by using the induction culture medium and application of the induction culture medium. According to the induction culture medium, an improved MS culture medium is used as a basic culture medium, and the induction culture medium further comprises 0.1 g / L to 1 g / L of proline, 0.1 g / L to 1 g / L of arginine, 0.1 g / L to 1 g / L of glutamine, 10 mg / L to 70 mg / L of adenine sulfate, 1 mg / L to 10 mg / L of silver nitrate, 0.2 mg / L to 2 mg / L of 2, 4-D, 0.2 mg / L to 2 mg / L of Zetin and 0.2 mg / L to 2 mg / L of Picloram. According to the method, the somatic embryo occurrence rate can be remarkably increased by optimizing the induction culture medium of the embryogenic callus of the heat reclamation 628, the anther somatic embryo occurrence rate of the heat reclamation 628 of the rubber tree variety can be increased to 50% or above by utilizing the induction culture medium, and the cotyledon embryo occurrence rate can be increased to 20% or above.

To provide a beer-flavored beverage with superior body. [Solution] A beer-flavored beverage containing 10 mg / L or more of L-glutamine.

The invention relates to a protein consisting of an amino acid sequence in which at least one modification selected from the group consisting of the following [1] to [7] is introduced in the amino acid sequence represented by SEQ ID NO: 1: [1] a modification in which the amino acid residue at a position 103 is substituted with a lysine residue, [2] a modification in which the amino acid residue at a position 76 is substituted with a cysteine residue, [3] a modification in which the amino acid residue at a position 144 is substituted with a cysteine residue, [4] a modification in which the amino acid residue at a position 134 is substituted with a glycine residue, [5] a modification in which the amino acid residue at a position 222 is substituted with a methionine residue, [6] a modification in which the amino acid residue at a position 73 is substituted with a glutamine residue, and [7] a modification in which the amino acid residue at a position 203 is substituted with a threonine residue.

A method is provided for treating a subject with cancer using a recombinant polypeptide comprising a DRα1 domain containing a glutamine residue at the position corresponding to amino acid 45 of SEQ ID NO: 1 or SEQ ID NO: 2, or an antigenic peptide covalently bound to a portion thereof. In some cases, the subject has a tumor that is resistant to immune checkpoint blockade treatment and / or does not express a BRAF mutation. The present invention provides, for example, a method for treating a subject with cancer, comprising administering to the subject a therapeutically effective amount of a recombinant polypeptide comprising a DRα1 domain containing a glutamine residue at the position corresponding to amino acid 50 of SEQ ID NO: 1 or SEQ ID NO: 2, or an antigenic peptide covalently bound to a portion thereof; or a nucleic acid encoding the recombinant polypeptide.

This invention discloses a method for efficient genetic transformation of rice that overcomes genotype dependence and a dedicated culture medium, relating to the field of biotechnology. The dedicated culture medium comprises KCl, KH₂PO₄, MgSO₄·7H₂O, CaCl₂·2H₂O, disodium ethylenediaminetetraacetate, FeSO₄·7H₂O, MnSO₄·H₂O, ZnSO₄·7H₂O, H₃BO₃, KI, CoCl₂·6H₂O, CuSO₄·5H₂O, Na₂MoO₄·2H₂O, glutamine, aspartic acid, arginine, glycine, cyclohexanehexyl alcohol, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, and a carbon source. Using this dedicated culture medium can increase the number of rice cells containing the NiR gene. 375 S 453 The genetic transformation efficiency of haplotype rice varieties is of great significance for overcoming the bottleneck of rice genotype dependence, promoting functional genomics research and molecular breeding.

To provide use methods of compositions in drug production for inhibiting cancer cells having low asparagine synthetase (ASNS) expression.SOLUTION: The disclosure relates to a use method of a composition comprising an arginine reducing compound and an asparagine reducing compound for preparing a medicament to inhibit a cancer cell, where an arginase being the arginine reducing compound is specifically effective in combination use with an asparaginase being the arginine reducing compound for inhibiting proliferation of cancer cells, in particular, cells having low ASNS expression. Further, the medicament comprises a compound that reduces glutamine concentration such as an aminotransferase inhibitor.SELECTED DRAWING: Figure 1