24 results about "Total amino acids" patented technology

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The present application belongs to the technical field of fermentation engineering, and particularly relates to a sheep liver peptide composition and a preparation method thereof, which are helpful for tonifying qi and blood and relieving dysmenorrhea. The sheep liver peptide composition comprises the following components in mass fractions: sheep liver peptide powder 60-70 parts, red date powder 5-15 parts, ginger powder 3-8 parts, licorice powder 3-8 parts, rose powder 2-6 parts, inulin 5-10 parts, astragalus peptide 5-10 parts, polygonatum kingianum peptide 5-10 parts, pepper powder 1-3 parts, cassia powder 1-3 parts, turmeric powder 2-5 parts, vitamin C 0.5-2 parts, and vitamin E 0.5-2 parts. The sheep liver peptide powder prepared by the present application has high contents of protein, total amino acids, free amino acids and vitamin A. The sheep liver peptide composition prepared by compounding the sheep liver peptide powder with the other components has the effects of tonifying qi and blood and relieving dysmenorrhea.

The application belongs to the technical field of microorganisms, and discloses a lactic acid bacteria preparation for improving the yield and quality of waxy corn and application thereof. The active bacteria in the lactic acid bacteria preparation are Lactobacillus casei C-9 strains, and the strain preservation number of the C-9 strains is CGMCC No. 6734. The application is at least one selected from the following: A, improving the yield of waxy corn; B, improving the amylopectin content, soluble sugar content and total amino acid content in waxy corn kernels; C, improving the taste and flavor of waxy corn; and D, enhancing the stress resistance of waxy corn. The application uses Lactobacillus casei C-9 bacterial liquid for waxy corn planting, solves the problems of soil physicochemical property deterioration, soil microbial community imbalance, soil fertility decline and environmental pollution caused by chemical fertilizers through the combined mode of seed treatment, base fertilizer application, leaf surface spraying and root irrigation.

The invention relates to the technical field of poultry health assessment, in particular to a method for predicting and identifying the high fat deposition risk of the liver of a laying hen based on blood amino acid parameters. The method comprises the following steps: collecting a blood sample when the laying hen is in a metabolic state after absorption, detecting the total amino acid content and the alanine content in blood, and substituting blood parameters into a pre-established liver triglyceride prediction model to obtain the predicted liver triglyceride content, and comparing the prediction results to judge the high fat deposition risk of the liver of the laying hen. According to the method, the laying hens do not need to be slaughtered and sampled, the fat deposition state of the livers of the living laying hens can be rapidly evaluated, and the method is suitable for early screening and risk early warning of high fat deposition of the livers of the laying hens under the large-scale breeding condition.

The invention discloses application of a corn Zm00001d028750 gene in regulation and control of grain traits, and relates to the technical field of bioengineering, a nucleotide sequence of a coding region of the Zm00001d028750 gene is as shown in SEQ ID NO.1, and an amino acid sequence of a protein coded by the Zm00001d028750 gene is as shown in SEQ ID NO.2. The invention further discloses application of the corn Zm00001d028750 gene in regulation and control of grain traits and application of the corn Zm00001d028750 gene in regulation and control of grain traits. According to the application of the corn Zm00001d028750 gene in regulation and control of grain traits, the hundred-grain weight of corn is an important factor influencing the yield of corn, and along with the increase of the hundred-grain weight of corn, the higher the seed yield is, the higher the yield is; lysine is an essential amino acid for human beings and monogastric animals, and the eating and feeding values of corn can be remarkably improved by increasing the content of lysine and total amino acids.

The present invention relates to a composition comprising a peptide fraction, wherein: the peptide fraction comprises cysteine and methionine; the mass content of methionine of the peptide fraction is greater than 1% relative to the total amino acids of the peptide fraction; the cysteine of the peptide fraction is at least partially sulfonated, the degree of sulfonation of the cysteine being greater than 50%, preferably greater than 70%, and even more preferably greater than 80%.

The present invention relates to the technical field of organic fertilizers, and discloses an organic water-soluble fertilizer taking wood vinegar liquid as a raw material and a preparation method thereof. The organic water-soluble fertilizer is made from the following raw materials in parts by weight: 35 - 45 parts of purified wood vinegar liquid, 15 - 20 parts of potassium humate, 8 - 12 parts of compound amino acids, 5 - 8 parts of chelated medium and trace elements, 2 - 3 parts of synergist, 0.5 - 1 part of pH regulator, and 11 - 34.5 parts of deionized water. The purified wood vinegar liquid is obtained by subjecting the crude wood vinegar liquid of a biomass boiler to pretreatment, gradient distillation, and adsorption and impurity removal, wherein the acetic acid content is ≥12%, the tar content is ≤0.1%, the pH value is 3.5 - 4.0, and the density is 1.12 - 1.15 g / cm3; the compound amino acids are compounded from glycine, glutamic acid, and proline according to a mass ratio of 2:1:1, and the total amino acid content is ≥95%. The present invention not only solves the toxicity problem of direct application of wood vinegar liquid but also increases the concentration of effective components. At the same time, a small amount of phenolic substances retained during the distillation process and the chitosan oligosaccharide in the synergist act synergistically, increasing the control effect of the fertilizer on soil-borne diseases to more than 85%.

The invention relates to the technical field of organic fertilizers, and discloses an organic water-soluble fertilizer with wood vinegar as a raw material and a preparation method thereof.The organic water-soluble fertilizer is prepared from, by weight, 35-45 parts of purified wood vinegar, 15-20 parts of potassium humate, 8-12 parts of compound amino acid, 5-8 parts of chelated medium trace elements, 2-3 parts of a synergist, 0.5-1 part of a pH regulator and 11-34.5 parts of deionized water. The purified pyroligneous liquor is prepared from biomass boiler crude pyroligneous liquor through pretreatment, gradient distillation and adsorption impurity removal, the acetic acid content is larger than or equal to 12%, the tar content is smaller than or equal to 0.1%, the pH value is 3.5-4.0, and the density is 1.12-1.15 g / cm < 3 >; the compound amino acid is formed by compounding glycine, glutamic acid and proline according to the mass ratio of 2: 1: 1, and the total content of the amino acid is larger than or equal to 95%. According to the invention, the toxicity problem caused by direct application of the pyroligneous liquor is solved, and the concentration of effective components is improved; meanwhile, a small amount of phenolic substances reserved in the distillation process and chitosan oligosaccharide in the synergist have a synergistic effect, so that the control effect of the fertilizer on soil-borne diseases is improved to 85% or above.

The invention discloses application of a herba houttuyniae polysaccharide extract in a feed capable of improving intestinal health of piglets, the feed comprises the herba houttuyniae polysaccharide extract and a basic feed, and the application method of the herba houttuyniae polysaccharide extract comprises the following steps: adding the herba houttuyniae polysaccharide extract into the basic feed, and continuously feeding 21-28-day-old weaned piglets for 28 days, the intestinal flora structure of weaned piglets is adjusted through the houttuynia cordata polysaccharide extract, short-chain fatty acid secretion is promoted, and the intestinal morphology is improved, so that diarrhea prevention and intestinal health improvement are achieved; the herba houttuyniae polysaccharide extract is prepared from the following components in percentage by mass: 20 percent to 21.6 percent of herba houttuyniae polysaccharide, 5 percent to 10 percent of volatile oil, 25 percent to 35 percent of flavonoid, 10 percent to 15 percent of total amino acid, 3.1 percent to 6.1 percent of alkaloid, 3 percent to 10 percent of organic acid and 1.5 percent to 5.5 percent of sterol, and the herba houttuyniae polysaccharide is prepared from glucose, galactose, mannose, arabinose and xylose. The invention aims to improve the survival rate of early weaned piglets, shorten the breeding cycle of a farm and improve the reproductive performance.

The application relates to a production method and application of combined fermentation of bupleurum and lonicera japonica residues by bacillus velezensis and pediococcus acidilactici, and belongs to the technical field of biotechnology. The bupleurum and lonicera japonica residues are treated by adopting a process of enzymolysis first and then fermentation, wherein the composite enzyme comprises cellulase, beta-glucanase, xylanase, beta-mannanase and phytase; and the fermentation bacteria comprise bacillus velezensis and pediococcus acidilactici. The fermented bupleurum and lonicera japonica residues provided by the application have a high release amount of active ingredients (chlorogenic acid, forsythoside and baicalin), and the addition ratio in broiler chickens is 2.0%-4.0%. The application effect includes improving growth performance, improving the levels of anti-inflammatory factor IL-10 and serum immunoglobulin IgM of the body, improving the muscle quality and flavor characteristics of the breast muscle, and improving the contents of inosinic acid, intramuscular protein, flavor amino acid and total amino acid. The product can not only improve the yield and quality of livestock products, but also relieve the pressure on the environment caused by solid waste residues, and has a good market prospect.

The invention provides a grassland grazing sheep and grassland grazing mutton identification method and application thereof, and belongs to the technical field of food authenticity detection. The stable isotope values of delta 13C and delta 15N of the sheep neck hair are detected, and whether the sheep belongs to grassland grassland can be judged. The identification method for grassland grazing mutton provided by the invention comprises the following steps: detecting stable isotope values of delta 13C and delta 15N and the contents of fatty acid, flavor amino acid and total amino acid in mutton; if delta13C is less than or equal to-19.0 per mill, delta15N is more than or equal to 4.0 per mill, omega-6: omega-3 in fatty acid is less than or equal to 2.5, and flavor amino acid: total amino acid is more than or equal to 50%, determining that the mutton is grassland grazing mutton. According to the identification method, the essential characteristics of grassland grazing mutton are highlighted from a multi-index combined angle, the influence of factors such as variety, region, month age and man-made interference can be eliminated, and the identification precision reaches 100%.

The invention discloses aspergillus niger and application thereof in fermented corn steep liquor, and belongs to the technical field of strain screening. The mutagenic aspergillus niger FS-A-24 provided by the invention has good tolerance to high-concentration corn steep liquor, the growth time of the mutagenic aspergillus niger FS-A-24 on a 20% corn steep liquor flat plate is shortened, and the spore overgrowth time is shortened from 5 days of an original strain to 4 days; the time point at which the biomass is obviously increased in a 10% corn steep liquor liquid culture medium is advanced from about 72 hours of an original strain to about 60 hours; the total amino acid content of the mycelia is increased by 20.11% when the mycelia are fermented for 72 hours, and the content of various amino acids is increased to some extent.

This application discloses a method for predicting feed ingredient digestibility kinetic parameters based on near-infrared spectroscopy, relating to the field of animal nutrition science and technology. The method includes collecting various feed ingredients to form a calibration sample set; acquiring raw near-infrared spectral data of the calibration sample set using a near-infrared spectrometer; determining the cumulative release of glucose, nitrogen, and total amino acids during small intestinal digestion through in vitro simulated digestion experiments, and calculating the release rates during rapid digestion, non-rapid digestion, and the final release rate of the entire digestion process to obtain standard reference values ​​for nine digestibility kinetic parameters; establishing a standard prediction model for each parameter, using spectral data as the independent variable and the standard reference values ​​for the nine digestibility kinetic parameters as the dependent variable; for unknown feed ingredient samples, acquiring their near-infrared spectra and preprocessing them, inputting the processed data into the established model, and outputting the predicted values ​​for the nine digestibility kinetic parameters.

The application belongs to the technical field of biological fermentation, and discloses a method for efficiently preparing earthworm functional liquid by using earthworm intestinal endogenous probiotics and enzymes and the earthworm functional liquid. The method comprises the following steps: step 1: activating and expanding B. velezensis XY1, P. megaspora and Lactococcus lactis respectively to obtain B. velezensis XY1 bacterial liquid, P. megaspora bacterial liquid and Lactococcus lactis bacterial liquid; and step 2: inoculating the B. velezensis XY1 bacterial liquid, the P. megaspora bacterial liquid, the Lactococcus lactis bacterial liquid and a compound enzyme into earthworm slurry to obtain the earthworm functional liquid. The B. velezensis XY1, the P. megaspora, the Lactococcus lactis and the compound enzyme are used for synergistic fermentation to obtain the earthworm functional liquid, the content of total amino acids and small peptides is significantly improved, and the effect of the earthworm functional liquid can be further improved.

This invention discloses an amino acid assay kit based on the o-phthalaldehyde (OPA) method, its preparation method, and its application. Belonging to the field of amino acid detection and analysis technology, it is used for rapid and accurate quantitative detection of amino acids in samples. The amino acid assay kit can not only measure the total amino acid content but also analyze specific amino acids and dipeptides such as alanine glutamine, meeting diverse analytical needs. This amino acid assay kit is suitable for batch testing and supports high-throughput laboratory applications. By optimizing the reagent formulation and operating procedures, this invention effectively simplifies the detection steps, improves detection sensitivity and specificity, and fills the gap in the market for OPA-based assay kits. This invention can be widely applied in nutrition, medicine, clinical diagnostics, and drug development, providing a convenient and efficient amino acid analysis tool for life science and medical research.

The present invention relates to the cosmetic, non-therapeutic, oral use of keratin hydrolysates having a specific total amino acid profile as an agent for improving the quality and / or appearance of the skin and comprising at least 88% by weight of free amino acids relative to the total weight of the amino acids of the hydrolysates, the remaining amino acids of the hydrolysate are present in the form of peptides having a molecular weight of less than 800 Daltons or equal to 800 Daltons, and to a corresponding cosmetic treatment process.

This invention relates to the field of poultry feed technology, and more particularly to a diet for determining the loss of endogenous amino acids in poultry and its application. The diet provided by this invention, by mass percentage, comprises 12-14% total amino acids, 17-22% hydrolyzed casein, 16-20% glucose, 18-21.6% amylose, and 30-37.4% amylopectin, wherein the ratio of glucose to starch is 0.30-0.40, and the ratio of amylose to amylopectin is 0.55-0.65. This invention can effectively detect the loss of endogenous amino acids in the ileum of broilers while ensuring normal weight gain and digestive physiological status, thereby improving the accuracy of calculating standard ileal amino acid digestibility, helping to reduce the waste of protein feed resources in broilers, and achieving precision nutrition.

The invention relates to the technical field of microorganisms, in particular to pichia kudriavzevii SJTU-BEL-1 capable of producing single-cell protein and application of the pichia kudriavzevii SJTU-BEL-1. The pichia kudriavzevii SJTU-BEL-1 provided by the invention is a strain obtained by performing primary screening, secondary screening and identification on supermarket over-ripe strawberry residues, and when the pichia kudriavzevii SJTU-BEL-1 is cultured for 72 hours, the dry weight of thalli reaches 41.25 + / -0.69 g / L, which is obviously superior to that of other strains. The strain has great application potential in single-cell protein production, and can still maintain high-yield protein capacity under the condition of high-density fermentation; the total protein content and the total amino acid content are higher than those of a common yeast fermentation product, and good industrial fermentation potential and application prospects are achieved.

The invention provides a rhizoma polygonati polysaccharide extract weaned piglet feed additive and application thereof.The feed additive comprises a rhizoma polygonati polysaccharide extract, and the rhizoma polygonati polysaccharide extract comprises, by mass, 36%-49.8% of rhizoma polygonati polysaccharide, 3%-12% of saponin, 2%-8% of flavone, 30%-45% of total amino acid and 0.2%-1% of alkaloid. Polygonatum sibiricum polysaccharide has various pharmacological activities such as anti-inflammation and bacteriostasis, immunity regulation, tumor resistance, oxidation resistance, blood sugar and blood fat reduction and the like, the polygonatum sibiricum polysaccharide extract is reasonably added into weaned piglet basic feed breeding, and the application dosage of the polygonatum sibiricum polysaccharide extract is 150-600 mg per kg of weaned piglet basic feed; the feed antibiotic can replace feed antibiotics, and can effectively reduce the diarrhea rate of weaned piglets, regulate the intestinal flora structure and improve the abdominal and intestinal morphology of the weaned piglets, so that the survival rate of the weaned piglets in the early stage is increased, the breeding cycle of a farm is shortened, and the reproductive performance is improved.

The invention belongs to the technical field of natural product processing and nutritious food, and discloses a green prickleyash composition rich in essential amino acid and application of the green prickleyash composition. Fresh green prickleyash is used as a flavor core, pleurotus geesteranus enzymolysis powder is used as an essential amino acid strengthening core, edible mushroom source protein, composite auxiliary materials and amino acid activity strengthening components with precise proportion are matched, and the compound is prepared through an innovative process of double-raw-material synergistic enzymolysis, co-freeze-drying and step-by-step compounding. Essential amino acids of the composition account for more than or equal to 45% of total amino acids, the composition contains eight essential amino acids for human bodies, leucine is more than or equal to 2.0 g / 100g, threonine is more than or equal to 1.2 g / 100g, and the integrating degree with FAO / WHO recommended human body demand modes is more than or equal to 90%. Precise nutrition complementation is formed through the natural flavor and amino acid composition of green prickleyash and the high free amino acid characteristic of pleurotus geesteranus enzymolysis powder, a large number of artificially synthesized synergists are not needed, the bioavailability is high, and the compound can be applied to the fields of food seasoning, nutritional supplements and feed additives.

The invention discloses Aspergillus niger with high yield of mycelium protein, and belongs to the technical field of strain screening. According to the ARTP induced aspergillus niger strain FS-A-25 disclosed by the invention, more mycelium proteins can be generated by fermenting 10% of corn steep liquor, and the biomass is increased to 14.97 g / L from 11.20 g / L of an original strain and is increased by 33.66%; the total amino acid content in the mycelium is increased by 21.10% compared with the original strain, and the Trp content and the Met content are increased by 25.00% and 18.91% respectively compared with the original strain. Compared with the original strain, the equilibrium degree of amino acid distribution of the aspergillus niger FS-A-25 mycelium is improved.

The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide-producing bacteria.

The invention discloses a compound microbial agent for improving the quality of white tea and application of the compound microbial agent. The composite microbial agent SynCom21 comprises 21 specific microbial strains separated from rhizosphere soil of a tea tree. The microbial agent is applied to tea trees, the metabolic network of fresh leaves can be directionally regulated and controlled, specifically, after white tea is prepared, the content of theanine and the content of total amino acid can be synchronously and remarkably increased, and meanwhile the content of bitter and astringent substances such as total flavonoids and catechins is systematically reduced. The synergistic optimization of the composition of the key flavor substances can improve the sensory quality of the white tea, so that the white tea presents more prominent fresh and mellow taste and lower bitter taste. The invention provides an effective solution for accurately regulating and controlling the flavor of the tea leaves through a microbial technology.

This invention provides a farming method that improves the muscle quality of crayfish, including steps such as optimizing farming facilities, optimizing feed, and increasing crayfish activity. Crayfish raised under this method exhibit balanced textural characteristics, higher levels of total amino acids and flavor compounds in their muscle, better protein bioavailability, and lower saturated fatty acid content, meeting modern consumers' demands for healthy food. The farming method of this invention is simple, suitable for industrialized farming, and the crayfish raised under this method have superior flavor and texture, making them more appealing to the market.