223 results about "Total amino acids" patented technology

An extract from leaves of mulberry is disclosed. The extract has an IC₅₀ value sufficient to inhibit α-glucosidase I. The extract may comprise 5-40% (w/w) total imino sugars and 20-70% (w/w) total amino acids. The extract may reduce the production of melanin for the treatment of such ailments or diseases caused by pigmentation as freckle, chloasma, striae gravidarum, sensile plaque and melanoma. The extract may also control blood glucose level.

An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

The invention relates to a method for preparing bonito stick protein hydrolysate with an effect of reducing uric acid. The method comprises the following main steps of raw material heat treatment, restriction digestion, membrane separation-anion exchange chromatography-gel filtration chromatography separation, concentration and spray drying so as to obtain the bonito stick protein hydrolysate with an effect of reducing uric acid. The amino acid analysis indicates that the zymolyte peptide fragment primary amino acid sequence contains four amino acids, namely histidine, arginine, lysine and threonine, the total mass content of the four amino acids is 70 percent of the total amino acid content of zymolyte. MALDI-TOF-MS mass spectrum determines that the molecular weight of the main peptide effective ingredient is less than 700Da. In-vitro uric acid reduction experiments prove that the bonito stick protein hydrolysate has a remarkable effect of inhibiting generation of uric acid, and has an inhibition rate over 50 percent; an oteracil potassium molded hyperuricemic rat animal model indicates that the bonito stick protein hydrolysate can be used for remarkably reducing the level of serum uric acid and serum creatinine of rats, and shows a relatively good kidney protecting effect.

The invention relates to a feed additive of aquaculture industry, in particular to a meat quality flavor modifying agent for grass carp or carp and a preparation method thereof. Each kilogram of modifying agent comprises the following components: 30 to 50g of seaweed extract, 10 to 20g of combination of inosinic acid and guanylic acid, 20 to 30g of carnitine, 15 to 30g of glycin, 10 to 20g of alanine and the balance of zeolite powder. The preparation method comprises the following steps: firstly, crushing solid materials in the seaweed extract, the combination of inosinic acid and guanylic acid, the carnitine, the glycin, the alanine and the zeolite powder materials and sieving the crushed solid materials through a sieve with 80 to 100 meshes for later use; and secondly, mixing and stirring the crushed solid materials in the proportion to ensure that the coefficient of variation CV of the uniformity is less than 5 percent to obtain the product. The modifying agent can effectively improve protein content, content of total amino acids, content of essential amino acids and content of delicious amino acid in muscles of the grass carp and the carp.

The present invention is related to a pharmaceutical composition for modulating angiogenesis comprising a therapeutically effective amount of an angiogenesis modulating compound and a pharmaceutically acceptable excipient, wherein said modulating compound is a recombinant of caveolin-1, a nucleic acid encoding the partial or total amino acid sequence of caveolin-1, or an analogue thereof or a pharmacologically acceptable derivative thereof, a compound modulating the expression of caveolin-1, an agonist or an antagonist or a competitive inhibitor of caveolin-1, a recombinant hsp90, a nucleic acid encoding the partial or total amino acid sequence of hsp90 or an analogue thereof or a pharmacologically acceptable derivative thereof, a compound modulating the expression of hsp90, an agonist or an antagonist or a competitive inhibitor of hsp90, a recombinant of Akt, a nucleic acid encoding the partial or total amino acid sequence of Akt, or an analogue thereof or a pharmacologically acceptable derivative thereof, a compound modulating the expression of Akt, an agonist or an antagonist or a competitive inhibitor of Akt.

An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

The invention discloses a medicine combination of snakegourd peel extract and allium macrostemon extract. The medicine combination is composed of 50-90 parts of snakegourd peel extract and 10-50 parts of allium macrostemon extract by weight; wherein, the total amino acid content in the snakegourd peel extract is not less than 50 percent, if calculated according to arginine, and the total saponin content in the allium macrostemon extract is not less than 50 percent, if calculated according to dioscin. The combination has stable quality, and high content of effective ingredients; the technique of the preparation method of the invention is stable, and has good reproduction quality. Proved by pharmacological tests, the combination has better protective effect on acute myocardial ischemia caused by rat hypophysin and canine myocardial ischemia due to coronary artery ligation. The medicine combination of the invention has promising application prospect in the respect of treating cardiovascular system diseases.

The invention discloses a medicine combination with the function of lowering blood sugar level as well as a preparation method and application thereof. The existing medicines for lowering blood sugar level are mostly western medicines which have certain dependence and side effect. The active ingredients of the medicine combination comprises total alkaloid and total amino acid which are extracted and separated from any one or the composition of moraceae plants such as cortex mori radicis, ramulus mori, folium mori and mulberry; in all active ingredients of the extractive obtained from extraction and separation, the mass percentage of the total alkaloid is 9.8%-44.6%, the mass percentage of the total amino acid is 30.0%-70.4% and the combined content of the two account for more than 50% of the mass percentage composition of the extractive. In the invention, the total alkaloid and the total amino acid, the active ingredients extracted from the traditional Chinese medicine can achieve noticeable effect through combined use, features in fine synergistic effect and enjoys better blood-sugar-level lowering effect than when the total alkaloid and the total amino acid are independently used.

The invention relates to a wine-flavored pig feed and formula production and feeding methods thereof. The making method of feed fine fodder comprises the following steps of: activating 1% of fermentation agent, then mixing with 2% of brown sugar, 10% of rice flour, 37% of corn meal, 5% of soybean meal, 5% of sorghum powder, 5% of barley or wheat flour, 15% of wheat bran, 5% of fly maggot powder or blood meal and 15% of bean residue, then adding equal amount of water, uniformly stirring until the water content is optimally 50-60%, then adding to a fermentation tank, sealing and carrying out anaerobic fermentation. The making method of fermented straw coarse fodder comprises the following steps of: kneading corn straw, cutting into 3-7 cm in length, adding 1% of fermentation agent, adding 10% of corn meal, adding 1% of salt, adding water, mixing until the water content reaches optimally 60%. Feeding technique and feed formula are as follows: the feed is fed twice at 8-9 in morning and 4-5 in afternoon, and water is freely drunk in the whole day. By using the wine-flavored pig feed, the slaughtering rate of pig body is improved, water drop loss and body skin thickness of pork are reduced, and total amino acid content and fresh amino acid content in muscle are improved.

The invention discloses a truffle wine producing process which comprises the following steps: A: preparing truffle mother liquor, namely immersing truffle with water after crushing the truffle, and obtaining the truffle mother liquor after extraction; extracting for 12-16 hours at temperature of 45-55 DEG C according to a material-liquid ratio (mass ratio between the truffle and the water) of 1:4-1:6, filtering and concentrating the extracting solution till the ratio between truffle mass and truffle mother liquor volume is 1:1; and B: blending the truffle wine, namely mixing the truffle motherliquor, tartary buckwheat wine (alcohol content of 60%vol) and water according to a ratio of 10-20%:63-87%:3-7% (volume ratio), and then carrying out filtration. The truffle wine produced by the process is clear and transparent, does not have suspended substances, overflows fungus fragrance and has the special fragrance of the truffle wine; from the nutrient angle, the truffle wine is rich in total amino acid and polysaccharide, contains special effective components of the truffle and has an appropriate nutrient ratio; in addition, the truffle wine organically combines the effective components of the truffle with common wines, and has special nutrients and particular flavor.

Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

The invention discloses a broccoli leaf protein and a preparation method thereof. The broccoli leaf protein is prepared according to the following steps of: preparing juice by using at least one organ of stems, leaves and ball-flowers of the broccoli as a raw material; and carrying out acid treatment and heat treatment, or, carrying out acid treatment and flocculant treatment to on the juice to obtain a sediment which is broccoli leaf protein. The broccoli leaf protein is dark-green powder, and has special taste of the broccoli, crude protein content of not less than 40 percent and the total amino acid content of not less than 25 percent. The broccoli leaf protein can be used in the feed for replenishing the protein required by animals.

Nutritive proteins are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in whey protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in whey protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in whey protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

The invention relates to a compound protein amino-acid feed which mainly solves the technical problem that technical personnel in a feed industry applies a grasped digestible amino acid formulation technology to a livestock and poultry simple-stomach animal scale livestock farm which carries out self blending by using pre-mixing materials at present to substitute for a mealie- bean pulp type high-crude protein formulation technology which is used all the time at present and on the basis of total amino acids. The compound protein amino-acid feed comprises the following materials in percentage by weight: 14%-73.5% of conventional protein feeds, 26%-83% of unconventional protein feeds and 0.5%-3% of essential amino acids. According to the invention, compound protein amino acids are applied to the production practice proving of the livestock and poultry simple-stomach animal scale livestock farm which carries out the self blending by using the pre-mixing materials. The compound protein amino-acid feed disclosed by the invention can conserve the proteins and the amino acids, reduce the environmental pollution and sufficiently utilize the unconventional protein feed resources and is an environmental-friendly saving type feed.

The invention provides a Shuanghuanglian medicinal composition. A method for preparing the Shuanghuanglian medicinal composition comprises the following steps of: mixing and extracting honeysuckle and fructus forsythiae to obtain a honeysuckle and fructus forsythiae extract; extracting scutellaria baicalensis to obtain a scutellaria baicalensis extract; and mixing the honeysuckle and fructus forsythiae extract and the scutellaria baicalensis extract to obtain the Shuanghuanglian medicinal composition, wherein the weight ratio of the honeysuckle to the fructus forsythiae to the scutellaria baicalensis is 1:2:1; and the medicinal composition comprises the following components in percentage by weight: 20 to 40 percent of total flavone, 5 to 20 percent of total saponins, 20 to 30 percent of total organic acid, 8 to 18 percent of saccharides, 0.2 to 2 percent of total amino acid and 0.35 to 1.0 percent of phillyrin. In the Shuanghuanglian medicinal composition, active ingredients are extracted and separated from the honeysuckle, the fructus forsythiae and the scutellaria baicalensis, the impurities and the inactive ingredients are removed, each active ingredient is proportioned reasonably, and the content of chlorogenic acid and caffeic acid is strictly limited, so that curative effect is ensured and safety is improved.

The present invention provides an artificially synthesized antimicrobial polypeptide that does not occur in nature and an antimicrobial agent comprising this polypeptide as the main component. This polypeptide contains in its peptide chain one or more types of nuclear localization signal sequences (NLSs) and/or one or more units of sequences prepared by partially modifying the NLS(s). The one or more units of NLSs and/or modified NLSs contain at least 5 amino acid residues in total and amount to 30% or more of the total amino acid residues constituting the peptide chain.