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Broccoli leaf protein and preparation method thereof

A technology for broccoli and orchids, applied in the field of broccoli leaf protein and its preparation, can solve the problems of unsuitable extraction of broccoli leaf protein, achieve significant economic and ecological benefits, solve resource shortages, and reduce production costs

Inactive Publication Date: 2011-03-30
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many studies on the methods of extracting leaf protein from other plants such as alfalfa and locust leaves, the existing extraction methods are not suitable for extracting broccoli leaf protein due to the large differences in the physical and chemical properties of different plant proteins.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, extract broccoli leaf protein by heating under acidic conditions

[0015] 1. Raw material pretreatment

[0016] Grind the collected 100kg flower balls with broccoli stems and leaves during the harvest period into 1-2cm long debris with a green fodder grinder or hay cutter, beat with a beater, press through a screw press, and pass through a filter with a pore size of 2.0mm Remove and collect 51.4kg of extracted juice. The measured dry matter, crude protein and total amino acid contents in the juice are shown in Table 1.

[0017] Table 1 Determination results of nutrients in broccoli leaf juice

[0018] index

[0019] 2. Extraction of broccoli leaf protein

[0020] 1) Add hydrochloric acid to the above juice, adjust the pH of the juice to 3.0, heat it to 70°C with a heat exchanger, keep it warm for 9 minutes, and let it stand to cool;

[0021] 2) Use a filter bag made of industrial filter cloth or filter with a filter press, the filter aperture...

Embodiment 2

[0026] Embodiment 2, adding flocculant under acidic conditions to extract broccoli leaf protein

[0027] 1. Raw material pretreatment

[0028] Grind the collected 100kg flower balls with broccoli stems and leaves during the harvest period into 1-2cm long debris with a green fodder grinder or hay cutter, beat with a beater, press through a screw press, and pass through a filter with a pore size of 2.0mm Remove and collect 53.2kg of extracted juice. The measured dry matter, crude protein and total amino acid contents in the juice are shown in Table 3.

[0029] Table 3 Determination results of nutrients in broccoli leaf juice

[0030] index

[0031] 2. Extraction of broccoli leaf protein

[0032] 1) adding hydrochloric acid to the juice to adjust the pH of the juice to 3.5;

[0033] 2) Add 20mL of 0.5% sodium polyacrylate solution per 1000mL to the juice and let stand for 15 minutes;

[0034] 3) Use a filter bag made of industrial filter cloth or filter with a filt...

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PUM

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Abstract

The invention discloses a broccoli leaf protein and a preparation method thereof. The broccoli leaf protein is prepared according to the following steps of: preparing juice by using at least one organ of stems, leaves and ball-flowers of the broccoli as a raw material; and carrying out acid treatment and heat treatment, or, carrying out acid treatment and flocculant treatment to on the juice to obtain a sediment which is broccoli leaf protein. The broccoli leaf protein is dark-green powder, and has special taste of the broccoli, crude protein content of not less than 40 percent and the total amino acid content of not less than 25 percent. The broccoli leaf protein can be used in the feed for replenishing the protein required by animals.

Description

technical field [0001] The invention relates to broccoli leaf protein and a preparation method thereof. Background technique [0002] Broccoli is a vegetable crop with strong seasonality. The flower bulbs are harvested from November to March of the next year. The stems and leaves of the plants after the flower bulbs are harvested are piled up in the field as waste, which is seriously Pollution of the environment in the main broccoli producing areas. According to calculations, the yield of broccoli stems and leaves is generally 2.5-3 tons per mu. Only based on the 70,000-mu broccoli planting base in Shangpan Town, Linhai City, Zhejiang Province, the total amount can reach 175,000-210,000 tons. Excessive accumulation of broccoli stems and leaves seriously affects the virtuous cycle of the ecological environment of the soil plowing layer. The nitrogen and phosphorus elements produced by the decay of broccoli stems and leaves after excessive returning to the field and re-cultiv...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/14
Inventor 刘国华郑爱娟江宇王永东
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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