The invention discloses
grasshopper umami peptide and a preparation method and application thereof. The
amino acid sequences of the
grasshopper umami peptide are NGWGDY, NVTY, VVNY, NGSGL and GDRFL. The preparation method I comprises the following steps: (1) sequentially carrying out swelling, washing, Fmoc
protecting group removal and
condensation reaction on dichloro resin; and (2)
cutting and purifying. A second preparation method comprises the following steps: (1) cleaning,
blanching, air-
drying, crushing, sieving and
degreasing the locusts in sequence; (2) dissolving in water, adjusting the pH value of the solution, adding Alcalase, carrying out enzymolysis,
enzyme deactivation and
centrifugation, taking supernate, and carrying out
freeze drying; (3) dissolving in water, performing
ultrafiltration separation, collecting permeate, and concentrating; and (4) dissolving in water, carrying out gel
chromatography separation, collecting, and freeze-
drying to obtain the product. The
grasshopper umami peptide contains umami active fragments, has umami characteristics, has an umami threshold value lower than that of
sodium glutamate, can enhance umami of simulated broth to a certain extent, has potential umami presenting / umami increasing effects, can be used for umami increasing seasonings, and can also be compounded with other umami products for use.