63 results about "Larva" patented technology

The utility model provides a grading screen for breeding macrobrachium rosenbergii larvae, which comprises a base, an arch-shaped mounting frame is fixedly mounted at the top of the base, a grading screening mechanism for the macrobrachium rosenbergii larvae is arranged on the inner side of the arch-shaped mounting frame, a water collecting tank is fixedly mounted at the top of the base, and a collecting tank is arranged at the top of the water collecting tank. And the macrobrachium rosenbergii larva grading screening mechanism comprises three screening grooves which are vertically placed, nylon filter screens are fixedly installed at the bottoms of the screening grooves, inserting grooves are formed in the right sides of the screening grooves, baffles are arranged in the inserting grooves in a penetrating and inserting mode, and two circular connecting rings are fixedly installed on the inner side of an arch-shaped installation frame. The base is arranged to achieve installation of the water collecting tank, the water collecting tank is arranged to collect and discharge water, and the macrobrachium rosenbergii larva grading screening mechanism is arranged to achieve grading filtering of macrobrachium rosenbergii larva. Further, larvae of macrobrachium rosenbergii with different sizes are separated, so that the survival rate during subsequent culture can be increased.

The invention discloses a method for enhancing adhesion ability of coral larvae after enhancement and release. According to the method, coral larvae (such as coral plankton larvae) are exposed to a 40 mM calcium chloride (CaCl2) solution to be treated for 12-24 hours, and then enhancement and release are carried out. The treated larvae show remarkably enhanced adhesion ability after releasing, the accumulated adhesion rate within 3 days reaches 66.33% and is increased by 240% compared with that of a control group in the same period, and the average survival rate reaches 98.14%. Meanwhile, the transverse growth area and the budding number of attached larvae are remarkably increased, and the density of zooxanthellae, the photosynthetic efficiency and the chlorophyll content have no negative effects. The CaCl2 solution is used for successfully inducing the continuous attachment effect of the coral larvae, the method is easy and convenient to operate, low in cost and environmentally friendly, the attachment success rate of enhancement and release of the coral larvae and the subsequent growth performance of the larvae are remarkably increased, and the method is suitable for repairing and protecting a coral reef ecological system.

The application discloses an amphibian artificial bionical natural breeding device and a breeding method, and belongs to the technical field of breeding devices. The amphibian artificial bionical natural breeding device comprises a breeding pond, and the breeding pond comprises a pond body. A breeding area is arranged in the middle of the pond body. Inclined climbing areas are arranged on the two sides of the breeding area. The climbing areas are connected with the breeding area through transition areas. A plurality of breeding frames for animal breeding are arranged between the climbing areas and the transition areas along the length direction of the pond body. Overflow holes communicating with the breeding area are arranged at the bottom of the breeding frames. Climbing passages for adult animals to return to the road surface are arranged between the breeding frames. The breeding area is internally provided with an avoiding structure for larvae to avoid. The amphibian artificial bionical natural breeding device and the breeding method can solve the problems of high cost of artificial propagation of the existing Tianhai spiny salamander and poor environmental adaptability of adults.

The present application relates to a kind of compound moxidectin pour-on and its preparation method and application, specifically to a kind of compound moxidectin pour-on, component mass mass fraction ratio includes: 0.1-5 portion moxidectin, 5-30 portion benzimidazole anthelmintic, 30-70 portion solvent, 5-20 portion solubilizer, 0.1-2 portion antioxidant, 10-30 portion transdermal enhancer.The present application not only covers gastrointestinal nematode, tapeworm, fluke and other various parasites, but also has killing or inhibiting effect on larva, worm egg, solve the problem of single component anthelmintic spectrum narrow, specific parasite effect is not good.

The invention provides a Babylonia areolata larva disease-resistant cultivation method based on immunostimulation, and aims to solve the problems of frequent larva diseases and low survival rate in intensive cultivation. The method comprises the following steps: selecting Babylonia areolata of Thailand and Hainan populations for reciprocal cross to obtain hybrid filial generations; in the larva stage, a compound immunologic stimulant compounded by astragalus polysaccharide, peptidoglycan and beta-glucan is adopted, the concentration is accurately regulated and controlled according to the early, middle and late stages of the daughter larvae and the creeping stage, and alternate induction is conducted through water splashing and bait mixing and feeding; when the larva shell length is 30-50mm, vibrio harveyi sublethal infection is used for enhancing immune memory, and environmental parameters such as water temperature and salinity are synchronously regulated and controlled. Tests show that the method can significantly improve the growth performance, immune enzyme activity and disease resistance of larvae, the survival rate of cultivation reaches 95% or above, the natural morbidity is lower than 6%, and technical support is provided for large-scale healthy cultivation of Babylonia areolata.

The application belongs to the technical field of microorganisms, and provides a carogenic Enterobacter and application thereof in degrading solanum alkaloid. Enterobacter cancerogenus JLU1, which was preserved in the China General Microbiological Culture Collection Center on June 23, 2025, and has a preservation number of CGMCC No.34971. In the embodiment of the application, intestinal bacteria are separated by dissecting the tomato leafworm larva intestine, and then bacteria resistant to solanum alkaloid or even capable of degrading and utilizing solanum alkaloid are screened and identified, so that the role of the bacteria in the resistance of the tomato leafworm to solanum alkaloid is analyzed, and a foundation is laid for subsequent research on the resistance mechanism.

The application discloses a kind of bat moth larvae double bacteria synergistic infection methods, belong to microbial engineering and medicinal fungus cultivation technical field, comprising: 1-2 age larvae are preprocessed by containing first probiotic feed feeding, optimize intestinal microenvironment;3-4 age larvae are injected and infected using mixed bacterial liquid containing second probiotic and Chinese hairy hair fungus, promote spore germination and colonization;5 age and above larvae are stably cultivated by third probiotic spraying, whole process is matched with dynamic monitoring and threshold emergency control.The technology adopts age differentiation operation of low-age feeding-middle-age accurate injection-high-age spraying, matches each stage functional targeted probiotic, constructs suitable mycelium germination microenvironment by means of probiotic metabolite, inhibits miscellaneous bacteria pollution and provides adenosine synthesis precursor, realizes the excellent effect of infection rate improvement, mortality reduction, adenosine content improvement and effective content improvement, provides efficient feasible technical path for large-scale high-quality cultivation of cordyceps sinensis.

This utility model discloses an intelligent larval rearing device, comprising: a rearing box including at least one incubation tray driving mechanism; an incubation tray disposed on the surface of the incubation tray driving mechanism; a perforated plate disposed above the incubation tray; a perforated plate driving mechanism for driving the perforated plate closer to or further away from the incubation tray; a refrigeration and drying module for regulating the temperature and humidity inside the rearing box; an environmental control mechanism for regulating the temperature inside the rearing box and exchanging air with the outside; and a control module electrically connected to the perforated plate driving mechanism, the refrigeration and drying module, and the environmental control mechanism.

The invention discloses a macrobrachium rosenbergii fry breeding method based on concentrated natural seawater, and relates to the technical field of aquaculture breeding, and the method comprises the following steps: S1, selecting surface seawater of a sea area of the northern bay of Guangxi, and sequentially carrying out pretreatment, concentration treatment and retreatment to obtain concentrated seawater; the pretreatment comprises precipitation, precise filtration and sterilization; the concentration treatment adopts a reverse osmosis method or is combined with a double-effect evaporation method, and the seawater is concentrated to 1 / 3-1 / 5 of the original volume; the retreatment comprises the following steps: adjusting the pH value to 7.8-8.2, adding an EDTA-2Na chelating agent, and carrying out precise filtration; s2, performing differential precipitation, three-stage gradient filtration and compatibility pre-detection on the selected water source to ensure that the turbidity of dilution water is less than or equal to 3NTU, the pH is 7.5-8.0, the ammonia nitrogen is less than or equal to 0.02 mg / L, and the residual chlorine is equal to 0mg / L. According to the method, through whole-course water quality management and staged accurate feeding, efficient, stable and green breeding from larvae to juvenile shrimps is achieved.

The invention discloses a method for synergistically preventing and controlling tomato leaf miners by trichogramma and ladybirds based on a specific population and adaptation training, and belongs to the field of biological prevention and control of agricultural pests. The method comprises the following steps: releasing a trichogramma group consisting of a trichogramma chilonis Jilin population, a trichogramma chilonis Guangzhou population and trichogramma brevituba in a greenhouse for planting tomatoes; a ladybird group composed of the coccinella septempunctata and the harmonia axyridis is released on the tomato plants; providing rhopalosiphum padi as an alternative prey of ladybirds in the greenhouse; before the ladybird group is released, the ladybird group is subjected to feeding domestication of tomato leaf miners, and after domestication, the ladybird group is subjected to starvation domestication. According to the method, the whole-course coverage of an egg-larva damage chain of the tomato leaf miner is realized. Meanwhile, by supplying alternative preys to maintain ladybird populations, the problems that the blank exists in single natural enemy prevention and control, the effect is unstable, and pests are prone to outbreak are solved, and the continuity and reliability of the prevention and control effect are remarkably improved.

The invention belongs to the technical field of biocontrol microbial agents, and particularly relates to a soil-borne disease biocontrol microbial agent as well as a preparation method and application thereof. The soil-borne disease biocontrol microbial inoculum is prepared by compounding a bacillus velezensis fermentation broth and a brevibacillus beautiful fermentation broth according to a volume ratio of 1: (0.5-1.5). Wherein the fermentation liquor contains thalli. The bacillus velezensis is a YCH-H7 strain, the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.33510, and the preservation date of the bacillus velezensis is February 11, 2025. The brevibacillus beautiful is an NF2 strain. The corrected death rate of second-instar larvae treated by the soil-borne disease biocontrol microbial inoculum for 48 hours is 98.85%, the nematode egg incubation inhibition rate is 88%, and the corrected death rate of ditylenchus destructor treated for 4 days is 97.7%. The hypha growth inhibition rates of the soil-borne disease biocontrol microbial inoculum on kiwi fruit root rot pathogenic bacteria, pepper phytophthora blight pathogenic bacteria, tomato neck and root rot, cucumber root rot pathogenic bacteria and wheat stem rot pathogenic bacteria exceed 90%, and the soil-borne disease biocontrol microbial inoculum has obvious broad spectrum.

The invention discloses an sNPF gene and application of an inhibitor of the sNPF gene in prevention and control of beet armyworms, and belongs to the technical field of biology. The sNPF gene of the beet armyworm is silenced through an RNA interference technology, and the sNPF is found to play an important regulation role in ingestion, growth and development and energy metabolism of larvae; the sNPF gene silencing obviously reduces the feeding amount and the body weight growth rate of the larvae, and increases the death rate of the larvae under hunger stress; in addition, silence of the sNPF gene also significantly affects the content of glycometabolism substances in the larva body. The sNPF is found to be a key factor for regulating and controlling the physiological process of beet armyworm larvae, has the potential of serving as a new target for pest control, and provides a theoretical basis for prevention and control of beet armyworms.

The utility model discloses a combined vegetable pest luring and killing device, including luring and killing subassembly, luring and killing subassembly include setting's outer frame, the storage frame of intercommunication in the bottom surface of outer frame, open in the opening groove of outer frame both side walls, with the protective net of outer frame connection, set up in the electric shock net of protective net inboard, lure the switching subassembly, lure the switching subassembly and include the side frame of connection in the side wall of outer frame, the first installation frame of clamping in the side frame, set up in the first ultraviolet ray fluorescent tube of first installation frame, with the second installation frame of first installation frame connection, set up in the second ultraviolet ray fluorescent tube of second installation frame, the utility model discloses the mutual cooperation of luring and killing subassembly and larva switching subassembly's cooperation reaches in the process of using, once ultraviolet ray fluorescent tube breaks down and cannot lure the insect, at this moment, ultraviolet ray fluorescent tube is directly pushed and realizes switching, like this to the maintenance personnel without timely discovery lamp tube breakage, still can keep luring and killing effect.

The application discloses a high-fiber silkworm larva feed containing protease and a preparation method thereof, and belongs to the technical field of enzyme engineering and feed for animal feeding in biological agriculture and related industries, and comprises a protease preparation-mulberry branch powder compound powder, mulberry branch powder, corn powder, soybean meal powder, citric acid, inorganic salt and guar gum. The application utilizes the easy-pulverization characteristics of fresh mulberry branches, adopts a pulverization and drying mode, shortens the drying time of the mulberry branches, and the artificial feed containing sufficient cellulose is beneficial to the feeding of silkworm larvae, promotes the peristalsis of the feed in the silkworm body, and is beneficial to meeting the feeding habits, main nutrition and physiological growth requirements of the silkworm; the proportion of the corn powder is increased to provide the silkworm with the required sugars and sugar compounds; the guar gum has good water retention performance, the silkworm larva feed after cooking is not prone to drying and hardening, and the use of the protease preparation effectively supplements the protease in the silkworm larvae, and promotes the growth and development of the silkworm larvae.

The application discloses a pine nematode inhibition method based on pachyman substitute cultivation, and comprises the steps of destroying the survival carrier of pine wood nematode and intermediate host, substitute fermentation directional regulation of microbial environment and pachyman fungus biological control, wherein the substitute fermentation makes the pH of the substrate in the range of 5.0-6.5, and the microbial environment for inhibiting the hatching of pine wood nematode and monochamus alternatus egg, the development of larva and the reproduction of adult is constructed; especially, the pH of the substitute substrate after fermentation is maintained at 5.5-6.5, and the pachyman fungus is combined. Compared with the existing biological control method of diseased wood, the pine nematode inhibition rate can be increased from 60-70% to 100%, and the secondary transmission risk of pine nematode is significantly reduced. While realizing the harmless treatment of diseased wood and completely blocking the transmission of pine nematode, efficient and high-quality cultivation of pachyman is achieved, and the method is suitable for the large-scale treatment of pine wood nematode disease and the industrialized planting of pachyman genuine producing area.

The invention provides a device and a method for selecting substrates with suitable grain sizes for schizothorax fries. The device comprises a cylindrical water tank, a separation assembly and a life support system. The water tank is divided into a central larva fish throwing area and a plurality of fan-shaped substrate areas by partition assemblies, and gravel substrates with the same gradient particle size (1-30 cm) are laid in the symmetrical areas; the life support system maintains the water temperature to be 16-18 DEG C, the dissolved oxygen to be 6-7 mg / L and the water flow to be stable. The method comprises the steps that a substrate is laid, environment parameters are adjusted, 100-200 healthy larva fishes are put in, after adaptation, a baffle is pulled away so that the substrate can be freely selected, after one hour, the baffle is put back for partition counting, and the appropriate particle size is determined through a preference degree formula (P = (n / N) * 100%). Free selection and accurate counting are achieved through the detachable baffle, the problem of observation deviation of a traditional device is solved, and the result provides reference for sediment construction of a schizothorax spawning site.

The present application relates to a kind of bait feeding mode suitable for northern oyster larva breeding work, at the D type larva period of northern oyster, feed mixed bait of chrysomonad and chlorella.The present application significantly improves the growth rate and survival rate of long oyster larvae by feeding mixed bait of chrysomonad and chlorella, and shortens the breeding cycle.

Hatchling support systems and methods of use thereof. Hatchling support systems and methods of use thereof can employ a cradle in combination with a stabilizing band, wherein the cradle and stabilizing band provide gentle support to hatchling(s) supported in the hatchling support system. Hatchling support systems and methods of use thereof can include a cam comprising cam segments having different cam radii that can provide a gentle transition of support and restraint during loading and unloading of hatchlings from the hatchling support system. In certain instances, the compaction imparted to hatchlings in the hatchling support system having both a cradle and a stabilizing band can be adjusted.

This invention provides a method for constructing an efficient ecological algal community for indoor breeding of mud crabs. Based on the growth characteristics of mud crab zoea larvae and their requirements for water quality and nutrition at each stage, a method for constructing a superior microalgal community and a balanced bacterial-algal community in the mud crab breeding pond is proposed. Six novel algal combinations not previously used in mud crab breeding are used to replace native algae, supplemented with probiotics. A daily combination of algae, bacteria, and biological feed for each developmental stage of the larvae (Z1-C1) is designed, significantly improving the survival rate and efficiency of indoor mud crab breeding. Compared to traditional breeding methods that rely entirely on native algae, this artificially constructed algae is more efficient and reproducible, facilitating the standardization of indoor breeding. This invention promotes a refined management model, achieving a balanced bacterial-algal community, stable and controllable water environment, and avoiding the instability caused by complex and variable native algae. This is more conducive to the promotion of mud crab breeding in areas with poor natural water quality.

The invention discloses an indoor cultivation method for increasing the survival rate of larvae and juveniles of horse mackerels, and relates to the technical field of aquaculture. Pretreated rotifers, shadberry fruit extracts and 2, 4 '-dihydroxychalcone are added in the early larva fish breeding stage and the later larva fish breeding stage, active ingredients such as gulcomannan, proline, chlorogenic acid and potentilla discolor extracts are added in the juvenile fish breeding stage, and meanwhile environmental factors such as illumination and sound wave stimulation are regulated and controlled, so that the juvenile fish breeding efficiency is improved. The survival rate of the horse mackerels and the capability of resisting infection of lactococcus gasseri and vibrio anguillarum can be effectively improved, the investment of the breeding cost is reduced, and the used baits are simple, easy to obtain and simple to prepare.

The utility model discloses a low-temperature stress treatment device for larvae in tussah variety breeding, which relates to the technical field of tussah variety breeding and comprises a treatment box, the treatment box is of a double-layer structure, the inner layer is made of thermal insulation materials, the outer layer is made of metal materials, and sliding grooves are formed in two sides of the inside of the treatment box. A breeding mechanism is slidably mounted in the treatment box through a sliding groove, mounting plates are fixedly mounted between the two sides of the interior of the treatment box at equal intervals, and monitoring devices are fixedly mounted at the bottoms of the multiple mounting plates. According to the low-temperature stress treatment device for the larvae in the tussah breeding, provided by the utility model, the temperature control system, the humidity control system, the silkworm body monitoring system and the data recording system are arranged, so that the temperature, the humidity and the silkworm body state in the treatment box can be accurately controlled and monitored in real time; a reliable low-temperature stress treatment environment is provided for the breeding process of tussah varieties, and the selection pressure is increased.

The present application belongs to the technical field of gene editing, and particularly relates to a gene editing method for obtaining a bivalve adult editing body. Specifically, a target gene with an outward visible phenotype and an early development not lethal is taken as a marker, target sites sgRNAs are designed based on the marker, and then a Cas9 / sgRNAs editing body is constructed by means of an electrotransfection system, so that gene editing of the bivalve is realized and the adult editing body is obtained. The present application breaks through the technical bottleneck that the existing gene editing of the bivalve is limited to the larva development stage and the editing body is difficult to screen in adulthood, and provides a new technical system for creating the editing body germplasm of the bivalve. In addition to being applicable to oysters, the technical system is also applicable to other bivalves with small fertilized eggs, difficult microinjection and high larva culture mortality.

The invention belongs to the technical field of hermatypic coral cultivation, and particularly relates to a hermatypic coral asexual cultivation method based on hydranth separation and reattachment. According to the method provided by the invention, natural separation of the hydranth is induced, the defects of mechanical stripping or chemical induction are avoided, the separation rate of the hydranth is high, and the separated hydranth is high in form integrity rate and activity, so that a foundation is laid for subsequent reattachment. According to the method, the adhesion rate of the hydranths is increased through the pretreated adhesion matrix, the survival rate of the hydranths is maintained at about 80% after the hydranths are bred for 60 days in combination with appropriate environmental parameters and accurate feeding, and the period of the hydranths from being separated to being bred into transplantable larvae is greatly shortened through the specific treatment method. Meanwhile, complex equipment or expensive chemical reagents are not needed in the whole cultivation process, brine fairy shrimps easy to cultivate are adopted as bait, the source is wide, the cost is low, the method is suitable for large-scale application and popularization, a feasible technical scheme is provided for large-scale asexual cultivation of hermatypic coral, and the method has important significance on restoration and protection of a coral reef ecological system.

According to the technical scheme, the holothuria leucospilota larva metabolite rapid detector is characterized in that the holothuria leucospilota larva metabolite rapid detector comprises a metabolite rapid detector body, a protective shell is arranged at the upper end of the metabolite rapid detector body, a display screen is assembled on one side of the protective shell, and a temperature sensor is arranged in the display screen; at least one group of metabolite detection mechanism is assembled in the middle of the upper end surface of the metabolite rapid detector, and the metabolite detection mechanism is positioned in the protective shell. According to the structural design of the rapid detector for the metabolite of the holothuria leucospilota larva, the detection precision and efficiency can be remarkably improved, and the core advantage is reflected in multi-mechanism cooperative detection and omnibearing detection capability. And in the metabolite detection mechanism, the output ends of a plurality of metabolite detection visual cameras arranged around the upper end of a mounting plate penetrate through the mounting plate and form cooperative detection with metabolite detection contact probes of which the lower ends are regularly distributed, so that the detection data dimension is enriched, and the limitation of a single detection mode is avoided.

The application provides a larva capturing device and relates to the field of mosquito research, which comprises a capturing cylinder, a capturing connecting floating plate, a capturing driving water pump, a capturing connecting piece, a capturing connecting disc, a capturing connecting box, a capturing driving mechanism and a worm egg capturing mechanism; the capturing connecting floating plate is fixedly connected to the outer side of the upper end of the capturing cylinder; the capturing driving water pump is fixedly connected to the upper left side of the capturing connecting floating plate; the capturing connecting piece is fixedly connected to the middle position inside the capturing cylinder, and a sliding groove structure is arranged on the outer periphery of the capturing connecting piece; the capturing connecting disc is slidingly connected to the upper end inside the capturing cylinder; the capturing connecting box is fixedly connected to the lower end right side of the capturing connecting disc; the capturing driving mechanism is arranged inside the capturing cylinder; and the worm egg capturing mechanism is arranged below the capturing connecting disc, so that the efficiency of larva capturing is improved, the labor intensity of experimenters is reduced, and the problems of less larva quantity and low capturing efficiency in one-time capturing are solved.

The invention discloses a zebra fish-based spatial resolution lipidomics method, and belongs to the technical field of biological medicines. The zebrafish-based spatial resolution lipidomics method provided by the invention comprises the following steps: separating a zebrafish trunk from yolk; sample pretreatment and chromatography-mass spectrometry detection. According to the method, yolk is sucked by a pipettor, trunks are cleaned in batches by a vortex instrument, after the trunks and the yolk are pretreated, a sample is subjected to lipidomics detection by an ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbitrap high-resolution mass spectrometer, and further data analysis is performed. According to the method, the trunk and yolk samples which can be used for high-quality and high-coverage lipidomics analysis are obtained while high sample flux is maintained for the first time, and regionalized analysis of zebrafish embryo / larva lipid metabolism is realized. The method has the advantages of simple sample mechanism, low lipid signal interference, high sensitivity and the like, and can detect inrecognizable subtle changes and key lipid molecules in the whole sample.

The application discloses a kind of vitamin A, D fortified feed for promoting procambarus clarkia larva to shed shell and grow, belongs to the technical field of aquaculture feed.The protein source raw material fish meal, soybean meal, cottonseed protein, rapeseed meal, peanut meal and corn DDGS in the feed, sugar source raw material wheat flour and fat source raw material soybean oil, soybean phospholipid oil also include compound vitamin, compound mineral, sodium alginate, calcium dihydrogen phosphate, yeast powder, sodium chloride, choline chloride, chitosan, threonine, lysine, methionine.The key nutrient vitamin A in the feed is added to 9000~27000 IU / kg, and the addition amount of vitamin D is 6000~12000 IU / kg.The procambarus clarkia feed of the application is the feed obtained by reasonable deployment, can fully meet the vitamin nutritional requirement of procambarus clarkia larva, can effectively improve the growth performance and feed utilization rate of procambarus clarkia larva, and increase the output of aquaculture.