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220 results about "Larva" patented technology

A larva (plural: larvae /ˈlɑːrviː/) is a distinct juvenile form many animals undergo before metamorphosis into adults. Animals with indirect development such as insects, amphibians, or cnidarians typically have a larval phase of their life cycle.

Method for calculating migratory parent fish quantity by using fish early-stage resource quantity

The invention provides a method for calculating migratory parent fish quantity by using fish early-stage resource quantity, which comprises the following steps: collecting early-stage resource quantity data, migratory parent fish quantity data and various parameters, and processing the collected data to obtain a comprehensive data set; constructing a basic model for representing the relationship among the number of roes, the number of larva fishes and the number of migratory parent fishes in combination with various parameters; designing a model architecture of the basic model by using a long-short-term memory network to obtain an initial prediction model, and quantifying the initial prediction model by using a Bayesian method to obtain a calculation prediction model; in combination with an MCMC method, performing parameter estimation and model training on the reckoning prediction model to realize optimization of the reckoning prediction model; and calculating the number of migratory parent fishes by using the optimized calculation and prediction model. According to the method, multiple data sources can be combined, the ecological environment complexity, the time sequence dynamics and the prediction uncertainty are fully considered, and accurate calculation of the migratory parent fish quantity is achieved.
Owner:QINGHAI LAKE NAKED CARP RESCUE CENT

Stepped wild domestication device for sepiella maindroni

The invention discloses a sepiella maindroni stepped wild domestication device which comprises a plurality of net cages arranged from inside to outside, each net cage is provided with a plurality of meshes, and the meshes in different net cages are gradually increased from inside to outside in a stepped manner; a plurality of suspension type fish reefs are arranged in the net cage, each suspension type fish reef comprises a plurality of single bodies horizontally installed on the floating rope, and each single body is a shell with a plurality of small holes formed in the shell wall. An area which is flat in terrain, relatively gentle in water flow, rich in plankton and about 10 m in water depth is selected near a sea area to be released as a domestication field, the device is put into the domestication field, and in-situ wild domestication is carried out on sepiella maindroni larvae; and the predation capability, the capability of avoiding enemies and the capability of coping with environmental stress after entering a natural sea area are improved, so that the enhancement and release effects are improved.
Owner:ZHEJIANG OCEAN UNIV

Upwelling circulating water culture system for high-density culture of abalone larvae and use method

The invention relates to an upwelling circulating water culture system for high-density culture of abalone larvae and a use method. Relates to the technical field of abalone recirculating aquaculture. Comprising a circulating water system, a breeding barrel, an electric one-way check valve, an electric adjusting valve and a drain valve, a water outlet of the circulating water system is connected with a water inlet of the breeding barrel through a water inlet pipeline sequentially provided with the electric one-way check valve and the electric adjusting valve, and a water outlet of the breeding barrel is connected with the water inlet end of the circulating water system through a water return pipe. A water outlet branch is arranged between the electric one-way check valve and the breeding cylinder, and the drain valve is arranged on the water outlet branch. Based on the characteristics of abalone larva culture, a three-dimensional culture system with bottom water rising flow is designed, meanwhile, the water flow direction is controlled to rise in a one-way mode through the water pump and the electric one-way check valve, high-density accumulation and adhesion of fertilized eggs in local areas in the pond are reduced, the metamorphosis rate of abalone larvae subjected to abalone larva circulating water culture is greatly increased, and the survival rate of abalone larvae is increased. And high-density accumulation of fertilized eggs in a local space is avoided.
Owner:ZHEJIANG UNIV

Method and device for efficiently proliferating sepiella maindroni by combining grazing and raising

The invention provides a method and a device for efficiently proliferating sepiella maindroni by combining grazing and raising, and belongs to the field of aquatic animal proliferation. An efficient breeding method for sepiella maindroni integrating grazing and breeding comprises the following steps that an appropriate sea area is selected, fertilized eggs are bred in a breeding facility laid in the downstream direction of a normal fish breeding net cage, namely an empty net cage containing an artificial floating reef, the periphery of the empty net cage is provided with an algae-mussel flow blocking ecological rope, and a cover net is arranged above the empty net cage; the newly hatched cuttlefish can stay in the proliferation facility empty net cage for a long time due to the characteristics of loving reefs and drilling the net and strong space awareness, and slowly escape into natural water after gradually adapting to the proliferation environment. According to the proliferation method, cuttlefishes gradually adapt to the natural environment of the sea area, meanwhile, cuttlefish larvae are well protected enough in the fragile incubation initial stage through protection measures such as net cages, and therefore the proliferation survival rate and the maintenance efficiency are improved, and efficient proliferation is achieved.
Owner:ZHEJIANG OCEAN UNIV

Breeding method for hypoxia-resistant golden tiger hybrid spots

The invention provides a breeding method of hypoxia-resistant golden tiger hybrid spots, which comprises the following steps: carrying out artificial insemination on a female parent of epinephelus coioides and a male parent of epinephelus coioides to obtain fertilized eggs, carrying out 2.8-3.2 mg / L hypoxia screening on the female parent for more than three generations, and ensuring that the suffocation point is less than or equal to 0.9 mg / L. Performing staged low-oxygen incubation on the fertilized eggs, gradually reducing dissolved oxygen in an embryonic period, a protointestinal period and a membrane emergence period, performing specific sound wave stimulation in an opening period of larva fish, performing low-oxygen-resistant phenotype screening in a larva period, performing intermittent low-oxygen intensive training in a juvenile fish period, applying water flow stimulation before training, and performing low-oxygen-resistant phenotype screening in a membrane emergence period; during the period, a fortified feed containing gamma-aminobutyric acid, heme iron and other components is fed. According to the method, through multi-link collaborative innovation, the hypoxia resistance of the Jinhu hybrid spots is effectively improved, the survival rate of a target group under 2.5 mg / L dissolved oxygen reaches 93%, the suffocation point is reduced to 0.75 mg / L, meanwhile, gill filament development and growth are promoted, and an efficient technical scheme is provided for aquatic product breeding.
Owner:陵水晨海种业有限公司

Young salamander breeding device

The invention discloses a giant salamander larva breeding device in the field of giant salamander breeding, and the device comprises a breeding frame which is of a multi-layer structure; the breeding boxes are arranged on the interlayer platforms of the breeding frame, the breeding boxes are divided into a plurality of areas through isolation structures, and the isolation structures have a water passing function; the water circulation system is arranged on the breeding frame and is used for supplying water to the breeding box on each interlayer platform and recycling overflow water; the giant salamander larva breeding device has the advantages that by arranging the breeding box with the isolation structure and the water circulation system, food protection of giant salamander larvae in the breeding process can be greatly avoided, the survival rate of the giant salamander larvae is increased, the time of manual care is shortened, and more materials can be provided for releasing the giant salamander larvae outdoors.
Owner:GERMPLASM INNOVATION GRAND SCIENCE CENTER OF WESTERN CHINA (CHONGQING) SCIENCE CITY

Feed formula method for improving growth and development of larvae and juveniles of micropterus salmoides

The invention provides a feed formula method for improving growth and development of larvae and juveniles of micropterus salmoides by adding taurine. Based on the feed formula meeting the development nutritional requirements of the larvae of the micropterus salmoides, the taurine is added as a functional additive, the growth performance of the larvae of the micropterus salmoides can be remarkably improved after the feed formula is used for about 3 weeks, and the development of muscles and bones is effectively promoted. The yield of the largemouth bass is increased, the common problem of skeleton deformity in the larva stage can be effectively solved, meanwhile, the fry culture time is shortened, and the problem that the fry are short of supply is solved.
Owner:YANGTZE UNIVERSITY

Young leech breeding barrel

The utility model relates to the technical field of leech larva cultivation, in particular to a leech larva cultivation barrel which comprises a barrel body, a cultivation bin is arranged in the barrel body, a partition plate is arranged below the cultivation bin, a filter plate is fixedly installed on the side face of the partition plate, a rotating shaft is rotatably installed in the middle of the partition plate, and the rotating shaft is fixedly connected with a sediment scraping plate. A blocking plate is installed below the partition plate and the filter plate, and one end of the blocking plate is connected with a sleeve which is rotationally installed on the periphery of the rotating shaft. The precipitation bin is arranged below the partition plate, and the pollution discharge assembly is arranged in the precipitation bin, so that excrement of leeches and impurities in water can be precipitated on the partition plate, fall onto the filter plate through the precipitation scraper, fall into the precipitation bin and then are discharged out of the barrel through the pollution discharge assembly, and the water in the barrel does not need to be completely discharged; after all the leeches are taken out, impurities in the barrel are removed, and growth of the leeches is not affected.
Owner:ANHUI LAISEN BIOTECHNOLOGY CO LTD

Muscle-driven fruit fly larva imitating intelligent body modeling and layered motion control method

The invention discloses a muscle-driven fruit fly larva-imitating intelligent body modeling and hierarchical motion control method, which comprises the following steps of: modeling a fruit fly larva multi-body-segment all-soft body based on a spring mass point method, modeling a muscle tension calculation node based on a muscle length-speed-tension nonlinear model, modeling a static fluid skeleton based on a volume constraint algorithm, and performing hierarchical motion control on the static fluid skeleton. And forming a drosophila larva imitating intelligent body under the simulation modeling framework. The method comprises the following steps: constructing a muscle bottom layer controller based on a von-mises cyclic distribution central mode generator for a fruit fly larva imitating intelligent body, and compressing a control dimension from a muscle number to a bottom layer controller parameter number; a reinforcement learning method is adopted to optimize a high-level strategy network for controlling parameters of a bottom-layer controller, so that a high-dimensional muscle system can drive a drosophila larva imitating intelligent body to generate multi-mode wriggling movement under the coordination of the hierarchical movement control method. The invention provides a novel structural design and a novel control algorithm, which are beneficial for promoting the application of the soft robot in the fields of industry, medical treatment and the like.
Owner:ZHEJIANG UNIV

Cultivation method for increasing survival rate of monopterus albus fries

The invention relates to the technical field of monopterus albus fry breeding, and discloses a breeding method for improving the survival rate of monopterus albus fries, which comprises the following steps: (1) egg yolk breeding period: placing water hyacinth in a hatching frame, placing an air dispersing stone at the bottom of the frame, keeping a micro-flowing water state, breeding the monopterus albus fries in the hatching frame at 26-28 DEG C for 5-7 days after the monopterus albus fries are hatched out of membranes, after the egg yolk is completely absorbed, the larva fish later-stage cultivation is performed; (2) at the later stage of fry rearing, the fry are fed with zooplankter live bait for 2-3 days in an open mode; feeding the larva fish for domestication feeding for 3-5 days through tubificidae feeding after the larva fish is opened; (3) a cage culture period: firstly replacing the hatching frame with an indoor cage, continuing to feed with tubificidae for 7-10 days, and then transferring the larva fish into an outdoor cage to feed with tubificidae for 3-5 days; (4) a food conversion culture period: performing food conversion on the larva fish by using food conversion baits formed by mixed baits of tubificidae and micro-capsule materials; the feeding rate is 5-10%, and fish diseases are prevented and controlled daily. According to the scheme, the survival rate of ricefield eel fries is effectively increased to 70%, and the economic benefit of breeding is remarkably increased.
Owner:重庆市水产科学研究所(重庆农垦农产品质量安全检验检测站)

Shellfish larva breeding system with automatic identification and feeding functions and breeding method of shellfish larva breeding system

The invention belongs to the field of aquaculture engineering, and particularly relates to an automatic identification and feeding shellfish larva cultivation system and method, and the system comprises a larva circulating water cultivation tank which is used for accommodating shellfish larvae and a circulating water body; the larva image acquisition device is used for acquiring larva image data and sending the larva image data to the control system; the peristaltic pump is used for receiving the control of the control system and quantitatively adding the bait algae liquid; the algae density sensor is used for collecting real-time microalgae concentration; the control system is used for receiving the image data of the larva image acquisition device and analyzing and acquiring the number of larvae and the shell length of the larvae; data of the algae density sensor is also received and converted into real-time microalgae concentration; meanwhile, according to the obtained data, the amount of bait algae liquid needing to be supplemented is calculated, the peristaltic pump is controlled to be started, and feeding is executed. The accurate feeding control system for the shellfish larvae is innovatively researched and developed, and the larva feeding amount is accurately controlled on the basis of the constructed accurate feeding preset model.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Breeding system for cherax quadricarinatus larvae

The utility model relates to a cherax quadricarinatus larva cultivation system which comprises a larva cultivation tank extending in the length direction of the cherax quadricarinatus larva cultivation system. The hanging rope extends in the length direction, is arranged in the larva culture tank, is located on the upper side of a water body of the larva culture tank and is used for hanging cherax quadricarinatus larvae; the oxygen pumping device is used for pumping oxygen into the water body; the filtering device is used for circularly filtering water. The cherax quadricarinatus larvae are hung in the larva culture tank in a concentrated mode for cultivation, and intensive management and large-scale cultivation of the cherax quadricarinatus larvae are achieved. The volume of the water body in the larva culture tank and the filtering device is controlled and extremely limited, so that the use amount of a nutrient solution in the water body is reduced, low-cost nutrient enrichment is conveniently realized, the immunity and stress resistance of the cherax quadricarinatus larva are improved at low cost, and large-scale culture is facilitated.
Owner:ZHUHAI XINTENG AOLONG AQUATIC PRODUCTS TECHNOLOGY CO LTD

Application of Brazilian circular nematode FAR-1 protein as anti-nematode drug target

The invention discloses an application of a Brazilian strongylus FAR-1 protein as an anti-nematode drug target. A fluorescent ligand competitive binding experiment shows that the Japanese strongylus Brazil FAR-1 protein has the capability of binding various fatty acids and retinol; the Nbfar-1 gene is in high expression in the whole development stage of the Brazilian strongylus; nbfar-1 gene expression is inhibited through RNAi, the lipid content of larvae can be reduced, the adult egg laying rate, the egg hatching rate, larva development and adult epidermis formation are affected, and therefore growth and development of Brazilian strongylus are remarkably affected. Therefore, the Brazilian circular nematode FAR-1 protein can be used as a drug target for screening or identifying anti-parasitic nematode drugs, a method is provided for development of the anti-parasitic nematode drugs, and the Brazilian circular nematode FAR-1 protein has important significance on prevention and treatment of infection of parasitic nematodes.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Spleen laying controller special for larva transplanting machine

ActiveCN222639425UImprove spawning efficiencyeasy to operateBeehivesAnimal scienceHoneycomb
The utility model relates to a special comb laying controller for a larva transplanting machine, which comprises a honeycomb enclosure frame, a queen excluder and a laying controller outer cover, the honeycomb enclosure frame is positioned on the periphery of a honeycomb area in the special comb for the larva transplanting machine, the queen excluder is positioned in the honeycomb enclosure frame, two ends of the queen excluder are erected on the honeycomb enclosure frame, and the laying controller outer cover is buckled outside the honeycomb enclosure frame. And a plurality of fence windows are arranged on the yield controller outer cover. A queen bee activity area is provided outside a special honeycomb of a larva moving machine by adopting a laying controller, a queen bee is manually placed in each activity area, the queen bee and the activity space of the queen bee are isolated by a queen bee isolating plate, and each queen bee lays eggs in a respective corresponding honeycomb region, so that the problems that the queen bees on the same honeycomb are too many, the eggs are overly laid, and the time is wasted when the queen bees find positions everywhere can be avoided; the spawning efficiency is improved, and the injury caused by the queen bee fight can be avoided.
Owner:HANGZHOU JINGYAN ELECTRONICS TECH CO LTD

Cage culture device for aquaculture

The invention relates to the technical field of aquaculture equipment, and discloses an aquaculture cage culture device which comprises a floating ring, a first sliding groove is formed in the bottom of the floating ring, a second sliding groove is formed in the top of the floating ring, and a transverse moving machine is slidably connected to the inner wall of the second sliding groove. A sliding mechanism and a cleaning mechanism are arranged in the equipment, when the outer wall of the breeding net needs to be cleaned, a power source of a transverse moving machine and a motor is switched on, the transverse moving machine slowly slides along the inner wall of a second sliding groove at the moment, and in addition, the motor drives a first gear to rotate through a driving rod; a first gear drives a square column, a sliding ring and a second cylinder to rotate reversely through a second gear, at the moment, the first cylinder and the second cylinder clamp and roll the culture net, most of larvae on the culture net are physically rolled, and attachment of barnacle marine organisms to the culture net is reduced.
Owner:JIANGSU TIANSHENGYUAN ECOLOGICAL FARM CO LTD

Method for incubating larvae of macrobrachium rosenbergii by using underground well water

The invention provides a method for incubating larvae of macrobrachium rosenbergii by utilizing underground well water, which comprises the following steps: removing calcium and magnesium ions in the underground well water by adopting a chemical precipitation method to prepare treated well water; mixing the treated well water with external river water to form basic seedling culture water; basic seedling culture water is added into the seedling culture pond, then quicklime and bleaching powder are added, and the basic seedling culture water is disinfected; artificial salt is added into the disinfected basic seedling culture water, and artificial seawater is prepared; larvae of macrobrachium rosenbergii are stocked in artificial seawater, and breeding production is carried out. According to the method, through systematic integration of cooperative utilization of underground well water and external river water, a seasonal dynamic disinfection strategy and multi-dimensional seedling raising environment accurate regulation and control, the incubation effect of macrobrachium rosenbergii larvae is remarkably improved, and the physicochemical indexes of water quality are better, the growth of the larvae is faster, the metamorphosis development is faster, and the survival rate is higher.
Owner:ZHEJIANG DANSHUI FISHERY RESEARCH INSTITUTE (ZHEJIANG DANSHUI FISHERY ENVIRONMENTAL MONITORING STATION)

Crayfish larva transportation device

The utility model discloses a crayfish larva transportation device, relates to the technical field of crayfish larva transportation, solves the problems that an existing device is low in practicability and low in crayfish larva survival rate, and adopts the following scheme that an oxygenation box is assembled on the top face of a transportation vehicle, and a water tank is fixedly installed on the left side of the oxygenation box. Through the arrangement of the oxygenation box, the oxygenation pipes and the shrimp seed boxes, the four shrimp seed boxes are arranged on the oxygenation box, shrimp seeds of different specifications and sizes can be conveyed in a classified mode, then oxygen is provided for the shrimp seeds through the oxygenation pipes evenly distributed at the bottoms of the shrimp seed boxes, the situation that the shrimp seeds die due to oxygen dead corners in the shrimp seed boxes is prevented, practicability is high, and practicability is high. Meanwhile, through the arrangement of an output pipe, a circulating pipe and a filter box, the circulating pipe sucks water and impurities in the shrimp seed box into the filter box to be filtered, then clean water in the water tank is input into the shrimp seed box through the output pipe, it is guaranteed that the water in the shrimp seed box is clean, and the survival rate of shrimp seeds is increased.
Owner:YIBIN TONGLE AGRICULTURAL TECHNOLOGY CO LTD +1

Classification screen for larva breeding of macrobrachium rosenbergii

The utility model provides a grading screen for breeding macrobrachium rosenbergii larvae, which comprises a base, an arch-shaped mounting frame is fixedly mounted at the top of the base, a grading screening mechanism for the macrobrachium rosenbergii larvae is arranged on the inner side of the arch-shaped mounting frame, a water collecting tank is fixedly mounted at the top of the base, and a collecting tank is arranged at the top of the water collecting tank. And the macrobrachium rosenbergii larva grading screening mechanism comprises three screening grooves which are vertically placed, nylon filter screens are fixedly installed at the bottoms of the screening grooves, inserting grooves are formed in the right sides of the screening grooves, baffles are arranged in the inserting grooves in a penetrating and inserting mode, and two circular connecting rings are fixedly installed on the inner side of an arch-shaped installation frame. The base is arranged to achieve installation of the water collecting tank, the water collecting tank is arranged to collect and discharge water, and the macrobrachium rosenbergii larva grading screening mechanism is arranged to achieve grading filtering of macrobrachium rosenbergii larva. Further, larvae of macrobrachium rosenbergii with different sizes are separated, so that the survival rate during subsequent culture can be increased.
Owner:天津市农业发展服务中心

Volute type water taking pump capable of preventing shell vegetables from being attached

The utility model relates to the technical field of water pumps, in particular to a volute type water taking pump capable of preventing shell vegetables from being attached, an LED lamp strip is installed in a closed cavity defined by a square lamp groove in a water filtering box and the inner wall of a box body, and the shell vegetables are driven to grow, breed and move in the area through the phototaxis of the shell vegetables. Therefore, shell vegetables and larvae are prevented from entering the water filtering tank; furthermore, an ultraviolet lamp strip is mounted in a closed cavity formed by the annular lamp groove and the inner wall of the volute body, so that the shell vegetables and the larvae entering the volute body can be killed, the shell vegetables are prevented from being attached to the inner wall of the volute body or an impeller, and the larvae are also prevented from entering various pipelines at the rear end to grow; secondly, the water inlet electromagnetic valve and the water outlet electromagnetic valve can be closed, the buffer box is screwed off, the high-pressure cleaning assembly is started, the interior of the volute body is thoroughly cleaned once, and attached shell vegetables are washed away; and finally, the buffer box can be screwed off regularly, and the shell vegetables or other objects rushing into the buffer box can be poured out.
Owner:COLLEGE OF MOBILE TELECOMM CHONGQING UNIV OF POSTS & TELECOMM

Drosophila larva tumbling motion modeling and optimizing method based on material point method

The invention discloses a fruit fly larva tumbling motion modeling and optimizing method based on a material point method. Aiming at a collaborative design challenge of a larva rolling motion model and the problems that a traditional method depends on manual data measurement and is time-consuming in training, a neural mechanical model is designed in combination with biological characteristics of drosophila larvae, and an artificial neural network based on deep learning is constructed as a model controller for training; meanwhile, a hyper-parameter optimization method based on a TPE algorithm is designed. According to the method, the method is embedded into a microprogrammable framework, and high integration and efficiency optimization of the method are achieved. The method can effectively simulate and optimize the rollover motion behavior of the drosophila larva under limited computing resources. The soft body model with the biological characteristics of the drosophila larvae has the advantages of flexible movement and adaptability to complex environments. According to the method, the optimal control strategy in the rollover process of the drosophila larvae is explored, interaction between the drosophila larvae and a complex environment is simulated, and important reference is provided for design of a soft robot similar to the drosophila larvae in reality.
Owner:ZHEJIANG UNIV

Method for enhancing adhesion ability of coral larvae after enhancement and release

The invention discloses a method for enhancing adhesion ability of coral larvae after enhancement and release. According to the method, coral larvae (such as coral plankton larvae) are exposed to a 40 mM calcium chloride (CaCl2) solution to be treated for 12-24 hours, and then enhancement and release are carried out. The treated larvae show remarkably enhanced adhesion ability after releasing, the accumulated adhesion rate within 3 days reaches 66.33% and is increased by 240% compared with that of a control group in the same period, and the average survival rate reaches 98.14%. Meanwhile, the transverse growth area and the budding number of attached larvae are remarkably increased, and the density of zooxanthellae, the photosynthetic efficiency and the chlorophyll content have no negative effects. The CaCl2 solution is used for successfully inducing the continuous attachment effect of the coral larvae, the method is easy and convenient to operate, low in cost and environmentally friendly, the attachment success rate of enhancement and release of the coral larvae and the subsequent growth performance of the larvae are remarkably increased, and the method is suitable for repairing and protecting a coral reef ecological system.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI

Edodging device for blue crab larvae

The sheltering device is applied to the field of aquaculture and is characterized in that a circular ring connecting line is fixedly installed in a nursery pond, a movable circular ring buckle is connected to the surface of the circular ring connecting line in a sliding mode, a first connecting line is fixedly installed at the bottom of the movable circular ring buckle, and a second connecting line is fixedly installed at the bottom of the first connecting line. A floating ball is fixedly installed at the bottom of the first connecting line, a connecting ring is fixedly installed at the bottom of the floating ball, a connecting rod is clamped in the connecting ring, a bent piece is fixedly installed at the bottom of the connecting rod, and a buckling rod is fixedly installed at one end of the bent piece. And a second connecting line is fixedly installed at the bottom of the bent piece, a falling ball is fixedly installed at the bottom of the second connecting line, an avoiding netting is fixedly installed on the surface of the falling ball, a penetrating rope penetrates through the interior of the bottom of the avoiding netting, the shape of the netting is controlled through a lead block, and the using effect of the device is improved.
Owner:GUANGXI FUQUN SEAWATER SEEDLING PROPAGATION CO LTD

Recombinant virus vector capable of being directly fed and infected as well as construction method and application of recombinant virus vector

The invention belongs to the technical field of gene engineering, and particularly provides a recombinant virus vector capable of being directly fed and infected, the recombinant virus vector takes polyhedrosis virus as a skeleton, and comprises a nucleotide sequence for coding a target protein and a nucleotide sequence for coding an exogenous polyhedrosis virus polyhedrosis protein. According to the recombinant virus vector provided by the invention, infection can be initiated only by ingesting a plurality of virus-embedded polyhedrosis by insect larvae, the expression yield of target protein is obtained to the maximum extent, and enough inclusion bodies with polyhedrosis can be obtained for infecting the insect larvae. The insect expression protein has conformation closer to that of mammals, and the applicability of the protein is improved. The invention further provides a production method of the target protein, a protein expression place is transferred from insect cell culture in a culture dish to insect larva direct culture, the cost is low, the technical difficulty in the large-scale production process is greatly reduced, and the protein production cost is low.
Owner:WUHAN CHUQIANG BIOLOGICAL TECH CO LTD

Portable larva quantitative sampling device

The utility model relates to the technical field of sampling devices, and discloses a portable larva quantitative sampling device which comprises a sleeve, a mounting block is fixedly connected to the outside of the right end of the sleeve, two limiting grooves are formed in the mounting block, springs are fixedly connected to the interiors of the limiting grooves, a sliding block is fixedly connected to the other end of each spring, and the sliding block is fixedly connected to the right end of the sleeve. A pull rope is fixedly connected to one side of the sliding block, a clamping block is fixedly connected to the other side of the pull rope, a filter screen is installed in the end, close to the installation block, of the sleeve, and clamping grooves are formed in the two sides of the filter screen. According to the utility model, the filter screen can prevent impurities and part of larvae from entering the sleeve when the larvae in water are extracted, so that the pollution to the inner wall, the influence on the next sampling and the inaccuracy of the sampling result can be avoided, meanwhile, the filter screen can be quickly disassembled and assembled through the clamping component, the replacement is convenient, and the service life of the device is prolonged.
Owner:TIANJIN FISHERIES RES INST (TIANJIN FISHERIES TECH EXTENSION STATION BOHAI SEA FISHERIES RES CENT OF CHINESE ACAD OF FISHERIES SCI)

Method for efficiently producing triploid oysters

The invention discloses a method for efficiently producing triploid oysters, and belongs to the technical field of aquaculture breeding. The method comprises the following steps: S1, obtaining sperms and ova of diploid oysters, and carrying out sperm-ova mixed fertilization; s2, performing induction treatment on the fertilized eggs to inhibit release of a second polar body; s3, collecting fertilized eggs subjected to induction treatment, cleaning, and hatching to a larva stage; s4, 24-72 hours after fertilization, screening based on larva individual size differences, and collecting a target larva population; and S5, culturing the larvae screened in the step S4. According to the method provided by the invention, even if the initial induction rate is only about 70%, the larva population with the triploid rate higher than 90% can be stably obtained finally, the hatching rate and the survival rate are high, the method is easy and convenient to operate, and the requirement for induction conditions is low; the method is suitable for triploid preparation of precious oyster varieties in which a large number of fertilized eggs are difficult to obtain under strong induction conditions or oyster strains with important economic characters.
Owner:YANTAI UNIV

Artificial bionics natural breeding device and method for amphibians

ActiveCN120937814BClimate change adaptationAnimal husbandryFisheryEchinotriton chinhaiensis
The application discloses an amphibian artificial bionical natural breeding device and a breeding method, and belongs to the technical field of breeding devices. The amphibian artificial bionical natural breeding device comprises a breeding pond, and the breeding pond comprises a pond body. A breeding area is arranged in the middle of the pond body. Inclined climbing areas are arranged on the two sides of the breeding area. The climbing areas are connected with the breeding area through transition areas. A plurality of breeding frames for animal breeding are arranged between the climbing areas and the transition areas along the length direction of the pond body. Overflow holes communicating with the breeding area are arranged at the bottom of the breeding frames. Climbing passages for adult animals to return to the road surface are arranged between the breeding frames. The breeding area is internally provided with an avoiding structure for larvae to avoid. The amphibian artificial bionical natural breeding device and the breeding method can solve the problems of high cost of artificial propagation of the existing Tianhai spiny salamander and poor environmental adaptability of adults.
Owner:CHINA JILIANG UNIV

Pseudomonas aeruginosa 24F6 and application thereof

The invention discloses pseudomonas aeruginosa 24F6 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a pseudomonas aeruginosa strain 24F6 with the preservation number of CGMCC No.35032. The chicken feather degradation rate is 86%, and the wool degradation rate is 40%. The specific activity of the strain cultured for 24 hours under the conditions that the pH value is 7.0 and the temperature is 40 DEG C is 119.67 U / mg. The invention also relates to application of the keratin degrading agent in preparation of keratin degrading products. The pseudomonas aeruginosa 24F6 has the following advantages that a plurality of bacteria are separated from the excrement of the larva of the costustomoth, strains with different keratin degradation capacities are screened out through a keratin selective culture medium, and the pseudomonas aeruginosa 24F6 is a strain which is reported for the first time and has unique keratin degradation characteristics; the specific activity of the strain under the optimal fermentation condition is obviously higher than that of reported similar strains, the degradation rate of natural chicken feathers is as high as 86%, and the strain shows good industrial application potential.
Owner:XINJIANG UNIVERSITY

Method for improving survival rate of larvae of lobsters in floating period

The invention relates to the technical field of lobster breeding, and discloses a method for increasing the survival rate of lobster larvae in the floating period. The method comprises the following steps: dividing a larva density monitoring area according to physical structure parameters of a culture pond, and continuously collecting initial water environment parameters to obtain water environment time sequence data; on the basis of the time sequence data, key water quality fluctuation nodes are marked by adopting a dynamic time axis generation technology, and a water quality change map with time marks is obtained; performing multi-dimensional environmental factor mapping processing by combining the spatial distribution information of the larva density monitoring area and the water quality change map to generate a larva living environment pressure matrix; introducing an adaptive learning mechanism, carrying out pattern recognition processing on a dissolved oxygen fluctuation section and an ammonia nitrogen accumulation point in the pressure matrix, and establishing an environmental stress response rule base; and dynamically analyzing the operation instruction of the breeding equipment by using the rule base, and generating a feeding path optimization strategy and an oxygenation intensity adjustment strategy.
Owner:SHENZHEN LONGKEYUAN AQUALTURE CO LTD

Artificial bionic natural breeding device and breeding method for amphibians

ActiveCN120937814AClimate change adaptationAnimal husbandryFisheryEchinotriton chinhaiensis
The invention discloses an artificial bionic natural breeding device and method for amphibians, and belongs to the technical field of breeding devices. An artificial bionic natural breeding device for amphibians comprises a breeding pond, the breeding pond comprises a pond body, a breeding area is arranged in the middle of the pond body, inclined crawling areas are arranged on the two sides of the breeding area, and the crawling areas are connected with the breeding area through transition areas. A plurality of breeding frames used for animal breeding are arranged between the crawling area and the transition area in the length direction of the pond body, overflowing holes communicated with the breeding area are formed in the bottoms of the breeding frames, crawling passages allowing adult animals to return to the road surface are arranged between the breeding frames, and an avoiding structure used for larva avoiding is arranged in the breeding area. By adopting the artificial bionic natural breeding device and breeding method for the amphibians, the problems of high cost and poor adaptive capacity to adult environment of the conventional artificial propagation of the spinodatl cephalus can be solved, and the artificial bionic natural breeding device and breeding method for the amphibians can be applied to the field of artificial propagation of the spinodatl cephalus.
Owner:CHINA JILIANG UNIV

Protease-containing high-cellulose silkworm larva feed and preparation method thereof

The invention discloses a protease-containing high-cellulose silkworm larva feed and a preparation method thereof, and belongs to the technical field of enzyme engineering of biological agriculture and related industries and feeds for feeding animals, and the protease-containing high-cellulose silkworm larva feed comprises protease preparation-mulberry shoot powder composite powder, mulberry shoot powder, corn flour, soybean meal, citric acid, inorganic salt and guar gum. The characteristic that fresh mulberry branches are easy to smash is utilized, the mode of drying after smashing is adopted, the drying time of the mulberry branches is shortened, the mulberry branches are artificial feed and contain sufficient cellulose, feeding of silkworm larvae is facilitated, peristalsis of the feed in silkworm bodies is promoted, and the requirements for feeding habits, main nutrition and physiological growth of silkworms are met; the proportion of corn flour is increased, and carbohydrates and carbohydrates required by the growth of the silkworms are provided; the guar gum has good water retention performance, the cooked silkworm larva feed is not easy to dry and harden, and the protease preparation is used to effectively supplement protease in silkworm larva bodies and promote the growth and development of the silkworm larvae.
Owner:HANGZHOU YIZIZI BIOTECHNOLOGY CO LTD