174 results about "Larva" patented technology

The invention discloses a sepiella maindroni stepped wild domestication device which comprises a plurality of net cages arranged from inside to outside, each net cage is provided with a plurality of meshes, and the meshes in different net cages are gradually increased from inside to outside in a stepped manner; a plurality of suspension type fish reefs are arranged in the net cage, each suspension type fish reef comprises a plurality of single bodies horizontally installed on the floating rope, and each single body is a shell with a plurality of small holes formed in the shell wall. An area which is flat in terrain, relatively gentle in water flow, rich in plankton and about 10 m in water depth is selected near a sea area to be released as a domestication field, the device is put into the domestication field, and in-situ wild domestication is carried out on sepiella maindroni larvae; and the predation capability, the capability of avoiding enemies and the capability of coping with environmental stress after entering a natural sea area are improved, so that the enhancement and release effects are improved.

The invention relates to an upwelling circulating water culture system for high-density culture of abalone larvae and a use method. Relates to the technical field of abalone recirculating aquaculture. Comprising a circulating water system, a breeding barrel, an electric one-way check valve, an electric adjusting valve and a drain valve, a water outlet of the circulating water system is connected with a water inlet of the breeding barrel through a water inlet pipeline sequentially provided with the electric one-way check valve and the electric adjusting valve, and a water outlet of the breeding barrel is connected with the water inlet end of the circulating water system through a water return pipe. A water outlet branch is arranged between the electric one-way check valve and the breeding cylinder, and the drain valve is arranged on the water outlet branch. Based on the characteristics of abalone larva culture, a three-dimensional culture system with bottom water rising flow is designed, meanwhile, the water flow direction is controlled to rise in a one-way mode through the water pump and the electric one-way check valve, high-density accumulation and adhesion of fertilized eggs in local areas in the pond are reduced, the metamorphosis rate of abalone larvae subjected to abalone larva circulating water culture is greatly increased, and the survival rate of abalone larvae is increased. And high-density accumulation of fertilized eggs in a local space is avoided.

The invention provides a method and a device for efficiently proliferating sepiella maindroni by combining grazing and raising, and belongs to the field of aquatic animal proliferation. An efficient breeding method for sepiella maindroni integrating grazing and breeding comprises the following steps that an appropriate sea area is selected, fertilized eggs are bred in a breeding facility laid in the downstream direction of a normal fish breeding net cage, namely an empty net cage containing an artificial floating reef, the periphery of the empty net cage is provided with an algae-mussel flow blocking ecological rope, and a cover net is arranged above the empty net cage; the newly hatched cuttlefish can stay in the proliferation facility empty net cage for a long time due to the characteristics of loving reefs and drilling the net and strong space awareness, and slowly escape into natural water after gradually adapting to the proliferation environment. According to the proliferation method, cuttlefishes gradually adapt to the natural environment of the sea area, meanwhile, cuttlefish larvae are well protected enough in the fragile incubation initial stage through protection measures such as net cages, and therefore the proliferation survival rate and the maintenance efficiency are improved, and efficient proliferation is achieved.

The invention provides a breeding method of hypoxia-resistant golden tiger hybrid spots, which comprises the following steps: carrying out artificial insemination on a female parent of epinephelus coioides and a male parent of epinephelus coioides to obtain fertilized eggs, carrying out 2.8-3.2 mg / L hypoxia screening on the female parent for more than three generations, and ensuring that the suffocation point is less than or equal to 0.9 mg / L. Performing staged low-oxygen incubation on the fertilized eggs, gradually reducing dissolved oxygen in an embryonic period, a protointestinal period and a membrane emergence period, performing specific sound wave stimulation in an opening period of larva fish, performing low-oxygen-resistant phenotype screening in a larva period, performing intermittent low-oxygen intensive training in a juvenile fish period, applying water flow stimulation before training, and performing low-oxygen-resistant phenotype screening in a membrane emergence period; during the period, a fortified feed containing gamma-aminobutyric acid, heme iron and other components is fed. According to the method, through multi-link collaborative innovation, the hypoxia resistance of the Jinhu hybrid spots is effectively improved, the survival rate of a target group under 2.5 mg / L dissolved oxygen reaches 93%, the suffocation point is reduced to 0.75 mg / L, meanwhile, gill filament development and growth are promoted, and an efficient technical scheme is provided for aquatic product breeding.

The invention belongs to the field of aquaculture engineering, and particularly relates to an automatic identification and feeding shellfish larva cultivation system and method, and the system comprises a larva circulating water cultivation tank which is used for accommodating shellfish larvae and a circulating water body; the larva image acquisition device is used for acquiring larva image data and sending the larva image data to the control system; the peristaltic pump is used for receiving the control of the control system and quantitatively adding the bait algae liquid; the algae density sensor is used for collecting real-time microalgae concentration; the control system is used for receiving the image data of the larva image acquisition device and analyzing and acquiring the number of larvae and the shell length of the larvae; data of the algae density sensor is also received and converted into real-time microalgae concentration; meanwhile, according to the obtained data, the amount of bait algae liquid needing to be supplemented is calculated, the peristaltic pump is controlled to be started, and feeding is executed. The accurate feeding control system for the shellfish larvae is innovatively researched and developed, and the larva feeding amount is accurately controlled on the basis of the constructed accurate feeding preset model.

The utility model relates to a cherax quadricarinatus larva cultivation system which comprises a larva cultivation tank extending in the length direction of the cherax quadricarinatus larva cultivation system. The hanging rope extends in the length direction, is arranged in the larva culture tank, is located on the upper side of a water body of the larva culture tank and is used for hanging cherax quadricarinatus larvae; the oxygen pumping device is used for pumping oxygen into the water body; the filtering device is used for circularly filtering water. The cherax quadricarinatus larvae are hung in the larva culture tank in a concentrated mode for cultivation, and intensive management and large-scale cultivation of the cherax quadricarinatus larvae are achieved. The volume of the water body in the larva culture tank and the filtering device is controlled and extremely limited, so that the use amount of a nutrient solution in the water body is reduced, low-cost nutrient enrichment is conveniently realized, the immunity and stress resistance of the cherax quadricarinatus larva are improved at low cost, and large-scale culture is facilitated.

The invention discloses an application of a Brazilian strongylus FAR-1 protein as an anti-nematode drug target. A fluorescent ligand competitive binding experiment shows that the Japanese strongylus Brazil FAR-1 protein has the capability of binding various fatty acids and retinol; the Nbfar-1 gene is in high expression in the whole development stage of the Brazilian strongylus; nbfar-1 gene expression is inhibited through RNAi, the lipid content of larvae can be reduced, the adult egg laying rate, the egg hatching rate, larva development and adult epidermis formation are affected, and therefore growth and development of Brazilian strongylus are remarkably affected. Therefore, the Brazilian circular nematode FAR-1 protein can be used as a drug target for screening or identifying anti-parasitic nematode drugs, a method is provided for development of the anti-parasitic nematode drugs, and the Brazilian circular nematode FAR-1 protein has important significance on prevention and treatment of infection of parasitic nematodes.

The invention relates to the technical field of aquaculture equipment, and discloses an aquaculture cage culture device which comprises a floating ring, a first sliding groove is formed in the bottom of the floating ring, a second sliding groove is formed in the top of the floating ring, and a transverse moving machine is slidably connected to the inner wall of the second sliding groove. A sliding mechanism and a cleaning mechanism are arranged in the equipment, when the outer wall of the breeding net needs to be cleaned, a power source of a transverse moving machine and a motor is switched on, the transverse moving machine slowly slides along the inner wall of a second sliding groove at the moment, and in addition, the motor drives a first gear to rotate through a driving rod; a first gear drives a square column, a sliding ring and a second cylinder to rotate reversely through a second gear, at the moment, the first cylinder and the second cylinder clamp and roll the culture net, most of larvae on the culture net are physically rolled, and attachment of barnacle marine organisms to the culture net is reduced.

The invention provides a method for incubating larvae of macrobrachium rosenbergii by utilizing underground well water, which comprises the following steps: removing calcium and magnesium ions in the underground well water by adopting a chemical precipitation method to prepare treated well water; mixing the treated well water with external river water to form basic seedling culture water; basic seedling culture water is added into the seedling culture pond, then quicklime and bleaching powder are added, and the basic seedling culture water is disinfected; artificial salt is added into the disinfected basic seedling culture water, and artificial seawater is prepared; larvae of macrobrachium rosenbergii are stocked in artificial seawater, and breeding production is carried out. According to the method, through systematic integration of cooperative utilization of underground well water and external river water, a seasonal dynamic disinfection strategy and multi-dimensional seedling raising environment accurate regulation and control, the incubation effect of macrobrachium rosenbergii larvae is remarkably improved, and the physicochemical indexes of water quality are better, the growth of the larvae is faster, the metamorphosis development is faster, and the survival rate is higher.

The utility model discloses a crayfish larva transportation device, relates to the technical field of crayfish larva transportation, solves the problems that an existing device is low in practicability and low in crayfish larva survival rate, and adopts the following scheme that an oxygenation box is assembled on the top face of a transportation vehicle, and a water tank is fixedly installed on the left side of the oxygenation box. Through the arrangement of the oxygenation box, the oxygenation pipes and the shrimp seed boxes, the four shrimp seed boxes are arranged on the oxygenation box, shrimp seeds of different specifications and sizes can be conveyed in a classified mode, then oxygen is provided for the shrimp seeds through the oxygenation pipes evenly distributed at the bottoms of the shrimp seed boxes, the situation that the shrimp seeds die due to oxygen dead corners in the shrimp seed boxes is prevented, practicability is high, and practicability is high. Meanwhile, through the arrangement of an output pipe, a circulating pipe and a filter box, the circulating pipe sucks water and impurities in the shrimp seed box into the filter box to be filtered, then clean water in the water tank is input into the shrimp seed box through the output pipe, it is guaranteed that the water in the shrimp seed box is clean, and the survival rate of shrimp seeds is increased.

The utility model provides a grading screen for breeding macrobrachium rosenbergii larvae, which comprises a base, an arch-shaped mounting frame is fixedly mounted at the top of the base, a grading screening mechanism for the macrobrachium rosenbergii larvae is arranged on the inner side of the arch-shaped mounting frame, a water collecting tank is fixedly mounted at the top of the base, and a collecting tank is arranged at the top of the water collecting tank. And the macrobrachium rosenbergii larva grading screening mechanism comprises three screening grooves which are vertically placed, nylon filter screens are fixedly installed at the bottoms of the screening grooves, inserting grooves are formed in the right sides of the screening grooves, baffles are arranged in the inserting grooves in a penetrating and inserting mode, and two circular connecting rings are fixedly installed on the inner side of an arch-shaped installation frame. The base is arranged to achieve installation of the water collecting tank, the water collecting tank is arranged to collect and discharge water, and the macrobrachium rosenbergii larva grading screening mechanism is arranged to achieve grading filtering of macrobrachium rosenbergii larva. Further, larvae of macrobrachium rosenbergii with different sizes are separated, so that the survival rate during subsequent culture can be increased.

The utility model relates to the technical field of water pumps, in particular to a volute type water taking pump capable of preventing shell vegetables from being attached, an LED lamp strip is installed in a closed cavity defined by a square lamp groove in a water filtering box and the inner wall of a box body, and the shell vegetables are driven to grow, breed and move in the area through the phototaxis of the shell vegetables. Therefore, shell vegetables and larvae are prevented from entering the water filtering tank; furthermore, an ultraviolet lamp strip is mounted in a closed cavity formed by the annular lamp groove and the inner wall of the volute body, so that the shell vegetables and the larvae entering the volute body can be killed, the shell vegetables are prevented from being attached to the inner wall of the volute body or an impeller, and the larvae are also prevented from entering various pipelines at the rear end to grow; secondly, the water inlet electromagnetic valve and the water outlet electromagnetic valve can be closed, the buffer box is screwed off, the high-pressure cleaning assembly is started, the interior of the volute body is thoroughly cleaned once, and attached shell vegetables are washed away; and finally, the buffer box can be screwed off regularly, and the shell vegetables or other objects rushing into the buffer box can be poured out.

The invention discloses a method for enhancing adhesion ability of coral larvae after enhancement and release. According to the method, coral larvae (such as coral plankton larvae) are exposed to a 40 mM calcium chloride (CaCl2) solution to be treated for 12-24 hours, and then enhancement and release are carried out. The treated larvae show remarkably enhanced adhesion ability after releasing, the accumulated adhesion rate within 3 days reaches 66.33% and is increased by 240% compared with that of a control group in the same period, and the average survival rate reaches 98.14%. Meanwhile, the transverse growth area and the budding number of attached larvae are remarkably increased, and the density of zooxanthellae, the photosynthetic efficiency and the chlorophyll content have no negative effects. The CaCl2 solution is used for successfully inducing the continuous attachment effect of the coral larvae, the method is easy and convenient to operate, low in cost and environmentally friendly, the attachment success rate of enhancement and release of the coral larvae and the subsequent growth performance of the larvae are remarkably increased, and the method is suitable for repairing and protecting a coral reef ecological system.

The invention belongs to the technical field of gene engineering, and particularly provides a recombinant virus vector capable of being directly fed and infected, the recombinant virus vector takes polyhedrosis virus as a skeleton, and comprises a nucleotide sequence for coding a target protein and a nucleotide sequence for coding an exogenous polyhedrosis virus polyhedrosis protein. According to the recombinant virus vector provided by the invention, infection can be initiated only by ingesting a plurality of virus-embedded polyhedrosis by insect larvae, the expression yield of target protein is obtained to the maximum extent, and enough inclusion bodies with polyhedrosis can be obtained for infecting the insect larvae. The insect expression protein has conformation closer to that of mammals, and the applicability of the protein is improved. The invention further provides a production method of the target protein, a protein expression place is transferred from insect cell culture in a culture dish to insect larva direct culture, the cost is low, the technical difficulty in the large-scale production process is greatly reduced, and the protein production cost is low.

The utility model relates to the technical field of sampling devices, and discloses a portable larva quantitative sampling device which comprises a sleeve, a mounting block is fixedly connected to the outside of the right end of the sleeve, two limiting grooves are formed in the mounting block, springs are fixedly connected to the interiors of the limiting grooves, a sliding block is fixedly connected to the other end of each spring, and the sliding block is fixedly connected to the right end of the sleeve. A pull rope is fixedly connected to one side of the sliding block, a clamping block is fixedly connected to the other side of the pull rope, a filter screen is installed in the end, close to the installation block, of the sleeve, and clamping grooves are formed in the two sides of the filter screen. According to the utility model, the filter screen can prevent impurities and part of larvae from entering the sleeve when the larvae in water are extracted, so that the pollution to the inner wall, the influence on the next sampling and the inaccuracy of the sampling result can be avoided, meanwhile, the filter screen can be quickly disassembled and assembled through the clamping component, the replacement is convenient, and the service life of the device is prolonged.

The invention discloses a method for efficiently producing triploid oysters, and belongs to the technical field of aquaculture breeding. The method comprises the following steps: S1, obtaining sperms and ova of diploid oysters, and carrying out sperm-ova mixed fertilization; s2, performing induction treatment on the fertilized eggs to inhibit release of a second polar body; s3, collecting fertilized eggs subjected to induction treatment, cleaning, and hatching to a larva stage; s4, 24-72 hours after fertilization, screening based on larva individual size differences, and collecting a target larva population; and S5, culturing the larvae screened in the step S4. According to the method provided by the invention, even if the initial induction rate is only about 70%, the larva population with the triploid rate higher than 90% can be stably obtained finally, the hatching rate and the survival rate are high, the method is easy and convenient to operate, and the requirement for induction conditions is low; the method is suitable for triploid preparation of precious oyster varieties in which a large number of fertilized eggs are difficult to obtain under strong induction conditions or oyster strains with important economic characters.

The application discloses an amphibian artificial bionical natural breeding device and a breeding method, and belongs to the technical field of breeding devices. The amphibian artificial bionical natural breeding device comprises a breeding pond, and the breeding pond comprises a pond body. A breeding area is arranged in the middle of the pond body. Inclined climbing areas are arranged on the two sides of the breeding area. The climbing areas are connected with the breeding area through transition areas. A plurality of breeding frames for animal breeding are arranged between the climbing areas and the transition areas along the length direction of the pond body. Overflow holes communicating with the breeding area are arranged at the bottom of the breeding frames. Climbing passages for adult animals to return to the road surface are arranged between the breeding frames. The breeding area is internally provided with an avoiding structure for larvae to avoid. The amphibian artificial bionical natural breeding device and the breeding method can solve the problems of high cost of artificial propagation of the existing Tianhai spiny salamander and poor environmental adaptability of adults.

The invention discloses pseudomonas aeruginosa 24F6 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a pseudomonas aeruginosa strain 24F6 with the preservation number of CGMCC No.35032. The chicken feather degradation rate is 86%, and the wool degradation rate is 40%. The specific activity of the strain cultured for 24 hours under the conditions that the pH value is 7.0 and the temperature is 40 DEG C is 119.67 U / mg. The invention also relates to application of the keratin degrading agent in preparation of keratin degrading products. The pseudomonas aeruginosa 24F6 has the following advantages that a plurality of bacteria are separated from the excrement of the larva of the costustomoth, strains with different keratin degradation capacities are screened out through a keratin selective culture medium, and the pseudomonas aeruginosa 24F6 is a strain which is reported for the first time and has unique keratin degradation characteristics; the specific activity of the strain under the optimal fermentation condition is obviously higher than that of reported similar strains, the degradation rate of natural chicken feathers is as high as 86%, and the strain shows good industrial application potential.

The invention relates to the technical field of lobster breeding, and discloses a method for increasing the survival rate of lobster larvae in the floating period. The method comprises the following steps: dividing a larva density monitoring area according to physical structure parameters of a culture pond, and continuously collecting initial water environment parameters to obtain water environment time sequence data; on the basis of the time sequence data, key water quality fluctuation nodes are marked by adopting a dynamic time axis generation technology, and a water quality change map with time marks is obtained; performing multi-dimensional environmental factor mapping processing by combining the spatial distribution information of the larva density monitoring area and the water quality change map to generate a larva living environment pressure matrix; introducing an adaptive learning mechanism, carrying out pattern recognition processing on a dissolved oxygen fluctuation section and an ammonia nitrogen accumulation point in the pressure matrix, and establishing an environmental stress response rule base; and dynamically analyzing the operation instruction of the breeding equipment by using the rule base, and generating a feeding path optimization strategy and an oxygenation intensity adjustment strategy.

The invention discloses an artificial bionic natural breeding device and method for amphibians, and belongs to the technical field of breeding devices. An artificial bionic natural breeding device for amphibians comprises a breeding pond, the breeding pond comprises a pond body, a breeding area is arranged in the middle of the pond body, inclined crawling areas are arranged on the two sides of the breeding area, and the crawling areas are connected with the breeding area through transition areas. A plurality of breeding frames used for animal breeding are arranged between the crawling area and the transition area in the length direction of the pond body, overflowing holes communicated with the breeding area are formed in the bottoms of the breeding frames, crawling passages allowing adult animals to return to the road surface are arranged between the breeding frames, and an avoiding structure used for larva avoiding is arranged in the breeding area. By adopting the artificial bionic natural breeding device and breeding method for the amphibians, the problems of high cost and poor adaptive capacity to adult environment of the conventional artificial propagation of the spinodatl cephalus can be solved, and the artificial bionic natural breeding device and breeding method for the amphibians can be applied to the field of artificial propagation of the spinodatl cephalus.

The utility model discloses a portable electric larva killing particle spraying device which comprises a box body, a controller, a pesticide distributor, an air pump, a pesticide conveying pipe, a power source and a driving motor. A medicine cabin is arranged in the box body, the medicine distributor is arranged in the medicine cabin, an output shaft of the driving motor extends into the medicine cabin and is fixedly connected with the medicine distributor, a plurality of separation teeth which are arranged at intervals are arranged on the outer side of the medicine distributor in the circumferential direction, a medicine storage groove is formed between every two adjacent separation teeth, and a medicine feeding opening communicated with the medicine cabin is formed in the top of the box body; the medicine outlet end of the medicine cabin is connected with the medicine inlet end of the medicine conveying pipe, the medicine conveying pipe extends out of the box body, and the medicine outlet end of the medicine conveying pipe is a medicine spraying opening; and the air pump is arranged on the medicine conveying pipe. According to the device, the granular pesticide in the pesticide cabin of the device is driven by electric power to pass through the pesticide conveying pipe and is sprayed to the position of a water body where mosquitoes can breed from the pesticide feeding opening in a high-speed airflow spraying mode, the mosquitoes in the water body development stage can be killed, and the phenomenon that the pesticide conveying pipe is blocked can be avoided.

This invention relates to a fully automated system for hatching and supplying brine shrimp, comprising: a brine shrimp hatching and supply device for collecting and automatically supplying brine shrimp larvae; and a tilted transparent membrane window selectively detachable from the brine shrimp hatching and supply device, the brine shrimp hatching and supply device comprising: a hatching tank for brine shrimp larvae to hatch from eggs; a collection tank for collecting only larvae and connected to the hatching tank; and a quadrilateral central channel incidentally formed on the central portion of the rear wall of the hatching tank to spatially connect the hatching tank and the collection tank.

This invention discloses a multi-task control method for a fully soft robot inspired by a fruit fly larva. The method is designed based on the perception-motor-control neural circuit and the motion adaptation neural circuit of a fruit fly larva. Applied to a soft robot inspired by a fruit fly larva, this method enables the robot to possess multiple motion modes such as continuous peristalsis, turning, and obstacle avoidance, while efficiently completing control tasks such as target arrival and navigation. Furthermore, the control method can adaptively optimize the robot's motion rhythm using environmental feedback information and measure the decision uncertainty of the current state based on historical trajectories. In areas requiring precise perception, the soft robot can automatically slow down its motion rhythm to improve localization and detection capabilities, making it suitable for navigation exploration and micro-manipulation in complex environments. This method solves the problems of low efficiency, poor stability, and weak generalization ability of traditional control algorithms driving soft robots, providing an important reference for the design of control methods for fully soft robots in reality.

The invention discloses an improved agricultural pest larva detection method based on YOLOv8, and relates to the technical field of target detection. Comprising the following steps of: constructing an APL8 (Activated Protein Label 8) data set of eight types of common agricultural pest larvae, and constructing an APL8 (Activated Protein Label 8) data set of the eight types of common agricultural pest larvae; the method comprises the following steps of: constructing a YOLOv8-LQG detection model on the basis of a YOLOv8 model, in combination with a designed LHF (Learnable Hue Filter) module, adding a QDDL (Quadruple Downsampling Description Layer) and a GLCM (Global Local Context Module) which fuses global semantics and local textural features, and constructing the YOLOv8-LQG detection model on the basis of the YOLOv8 model; a YOLOv8-LQG model is trained; and the YOLOv8-LQG model is tested.

The present application relates to a kind of compound moxidectin pour-on and its preparation method and application, specifically to a kind of compound moxidectin pour-on, component mass mass fraction ratio includes: 0.1-5 portion moxidectin, 5-30 portion benzimidazole anthelmintic, 30-70 portion solvent, 5-20 portion solubilizer, 0.1-2 portion antioxidant, 10-30 portion transdermal enhancer.The present application not only covers gastrointestinal nematode, tapeworm, fluke and other various parasites, but also has killing or inhibiting effect on larva, worm egg, solve the problem of single component anthelmintic spectrum narrow, specific parasite effect is not good.

The invention relates to the technical field of biology, and discloses a cordyceps sinensis optimized culture medium and a culture method.The cordyceps sinensis optimized culture medium comprises a culture dish and further comprises a solid layer, a nutrition layer and an induction layer; the solid layer comprises a substrate filled in the inner bottom layer of the culture dish, and the substrate comprises 60%-70% of corncob powder, 15%-20% of wheat bran, 10%-15% of hepialus armoricanus larva residue powder, 0.5%-1% of monopotassium phosphate, 0.2%-0.4% of magnesium sulfate heptahydrate, 0.01%-0.05% of ferric sulfate and 5%-10% of calcium carbonate. Through the synergistic effect of the solid layer, the nutrition layer and the induction layer, the solid layer takes corncob powder, wheat bran and sawdust as substrates, provides physical support and water-holding and ventilation functions, and promotes hypha adhesion and expansion; the nutrient layer realizes selective permeation through a microporous filter membrane, so that solid matrix particles are prevented from settling to interfere with liquid flow, small-molecular nutrient substances such as glucose and amino acid are ensured to be efficiently transmitted to the matrix layer, and dynamic balance required by hypha metabolism is maintained.

The invention provides a Babylonia areolata larva disease-resistant cultivation method based on immunostimulation, and aims to solve the problems of frequent larva diseases and low survival rate in intensive cultivation. The method comprises the following steps: selecting Babylonia areolata of Thailand and Hainan populations for reciprocal cross to obtain hybrid filial generations; in the larva stage, a compound immunologic stimulant compounded by astragalus polysaccharide, peptidoglycan and beta-glucan is adopted, the concentration is accurately regulated and controlled according to the early, middle and late stages of the daughter larvae and the creeping stage, and alternate induction is conducted through water splashing and bait mixing and feeding; when the larva shell length is 30-50mm, vibrio harveyi sublethal infection is used for enhancing immune memory, and environmental parameters such as water temperature and salinity are synchronously regulated and controlled. Tests show that the method can significantly improve the growth performance, immune enzyme activity and disease resistance of larvae, the survival rate of cultivation reaches 95% or above, the natural morbidity is lower than 6%, and technical support is provided for large-scale healthy cultivation of Babylonia areolata.

The application belongs to the technical field of microorganisms, and provides a carogenic Enterobacter and application thereof in degrading solanum alkaloid. Enterobacter cancerogenus JLU1, which was preserved in the China General Microbiological Culture Collection Center on June 23, 2025, and has a preservation number of CGMCC No.34971. In the embodiment of the application, intestinal bacteria are separated by dissecting the tomato leafworm larva intestine, and then bacteria resistant to solanum alkaloid or even capable of degrading and utilizing solanum alkaloid are screened and identified, so that the role of the bacteria in the resistance of the tomato leafworm to solanum alkaloid is analyzed, and a foundation is laid for subsequent research on the resistance mechanism.

The present invention relates to an Octopus sinensis larva cultivation device based on upwelling and a using method thereof, belonging to the technical field of aquaculture. The device includes a cultivation pond and a partition net. The cultivation pond is a cube or a cuboid. The partition net is arranged inside the cultivation pond and is perpendicular to the bottom surface of the cultivation pond. The water inlet pipe enters from the bottom of the cultivation pond, extends horizontally to the partition net, and the water inlet is oriented towards the other side of the partition net. The connection between the side wall and the bottom surface of the cultivation pond on this side is arc-shaped. The water outlet is arranged on the same side as the water inlet pipe and is located at the upper part of the cultivation pond. A sieve net is provided on the drain outlet, and an air stone is arranged at the bottom on the same side as the water inlet pipe. The present invention provides a using method of the device, which simulates the upwelling existing in the natural habitat of Octopus sinensis larvae, provides the necessary water flow stimulation for the growth and development of the larvae, and promotes the growth and bottom resting of the larvae.

The present application relates to the field of aquaculture, and particularly relates to a method for hatching and cultivating fry of Takifugu obscurus, which comprises nine steps of concentrated hatching of fertilized eggs, separation of fertilized eggs, egg spreading, air control, water temperature control, water quality control, bait and feeding method, pool division, and desalination of fry. The present application creates a simple method for hatching and cultivating fry of Takifugu obscurus from fertilized eggs, and particularly changes the early larva carrying into the fertilized egg carrying in the technical scheme, breaks through the existing cognition, and creates a simple operation of moving the Takifugu obscurus from the conical hatching barrel into the flat bottomed fry cultivation pool before the membrane of the fertilized egg is broken, and a late embryo membrane breaking and fry cultivation technical scheme. The present application improves the Takifugu obscurus breeding efficiency and reduces the artificial breeding cost. The present application saves the breeding production cost, is simple to operate, and is easy to be accepted and popularized by people.