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72results about How to "Increase perversion rate" patented technology

Preparation method and application of sea bdellovibrio bacteriovorus ecological preparation

InactiveCN101781627AGuaranteed lytic activityPromote lysisBiocideBacteriaNutrient brothCulture mediums
The invention provides a preparation method and application of a sea bdellovibrio bacteriovorus ecological preparation, and relates to a sea microbial ecological preparation and a preparation method of a bdellovibrio bacteriovorus preparation for mariculture. The preparation method comprises the following steps of: preparing a host bacteria suspension; separating and purifying bdellovibrio bacteriovorus; and propagating and culturing bdellovibrio bacteriovorus. The preparation method is characterized in that after pseudomonas stutzeri is connected with an incline and is activated, adding physiological saline in a test tube to prepare the bacterial suspension; then propagating and culturing with nutrient bouillon; adding a flocculating agent in the obtained bacterial suspension; standing and precipitating after uniformly shaking; adding the physiological saline to obtain 1012 cfu / mL of host bacteria suspension; separating and purifying the bdellovibrio bacteriovorus of collected sea and bottom sediment samples with a double agar plate method; pouring culture media containing the host bacteria suspension and the collected samples into sea agar in the lower layer for culture after uniformly mixing; and adding the host bacteria and the bdellovibrio bacteriovorus obtained by separation into sterilized natural sea, and culturing until the concentration of the bdellovibrio bacteriovorus is 108 to 109 pfu / mL for future use.
Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Preparation method of Crassostrea hongkongensis fixation medium, Crassostrea hongkongensis fixation medium and application method of Crassostrea hongkongensis fixation medium

The invention discloses a preparation method of a Crassostrea hongkongensis fixation medium. The preparation method comprises the following steps that (1) a plurality of rectangular plastic sheets are made and a round hole with the radius being 3-5 mm is formed in each plastic sheet, wherein the length of the plastic sheets is 10-20 cm, the width of the plastic sheets is 2-5 cm and the thickness of the plastic sheets is 1-3 cm; (2) the plastic sheets are strung through a rope, a plastic tube is arranged between every two adjacent plastic sheets, the plastic tubes are arranged on the rope in a sleeved mode, and then the preparation process of the Crassostrea hongkongensis fixation medium is completed. The invention further discloses the Crassostrea hongkongensis fixation medium and an application method of the Crassostrea hongkongensis fixation medium. After being processed through oxalic acid, fresh water and the like, the Crassostrea hongkongensis fixation medium is used as an eyespot larva collection fixation medium; the Crassostrea hongkongensis fixation medium increases the metamorphosis rate of Crassostrea hongkongensis larvas, accelerates juvenile mollusk growth, improves the juvenile mollusk survival ability and increases the juvenile mollusk yield, has the advantages of being capable of being recycled multiple times, light, convenient to use, good in corrosion resistance and the like and is worthy of popularization and application.
Owner:GUANGXI ACADEMY OF FISHERY SCI

Litopenaeus vannamei parent prawn feed capable of replacing fresh and living bait

ActiveCN106306554AIncrease egg productionIncrease hatching rate and metamorphosis rateAnimal feeding stuffAccessory food factorsDistortionCalcium
The invention relates to a litopenaeus vannamei parent prawn feed capable of replacing a fresh and living bait, and belongs to the field of nutrients and feeds of aquatic animals. The feed comprises the following ingredients in percentage by weight: 40% of fish meal, 6% of casein, 10% of krill meal, 15% of mussel meal, 14.75% of high-gluten flour, 0.5% of cholesterol, 2% of phospholipid, 7% of fish oil, 1% of choline, 0.5% of astaxanthin, 0.75% of vitamin C, 1.5% of calcium hydrogen phosphate, 0.5% of complex vitamins, and 0.5% of composite mineral substances. According to the litopenaeus vannamei parent prawn feed disclosed by the invention, through a compounding ratio of various nutrient components and through selection of raw materials, a prepared compound feed can meet nutritional requirements of parent prawns for reproduction, and feeding effects in respects of an egg laying rate, a continuous egg laying rate, a hatching rate, a distortion rate, egg diameters and the like of the parent prawns fed with the litopenaeus vannamei parent prawn feed are not significantly different from those fed with fresh and living clamworms, so that the feed disclosed by the invention can replace the fresh and living bait for strengthening the nutrients of the parent prawns, and the problem that the parent prawns are infected with external disease sources when being fed with the fresh and living bait is solved.
Owner:邦普种业科技有限公司

Cultivation method of quasipaa spinosa tadpole metamorphosis larvae

The invention belongs to the field of aquaculture, and particularly relates to a cultivation method of quasipaa spinosa tadpole metamorphosis larvae. The method comprises a tadpole cultivation pool, ambient conditions, stocking of small tadpoles, a culture method and a method for promoting metamorphosis. The illumination intensity suitable for a tadpole breeding stage, the illumination intensity suitable for a tadpole metamorphosis stage and the illumination intensity for controlling the metamorphosis speed are optimized, so that according to the growth stage and the metamorphosis stage of tadpoles, characteristic nutrition is optimized, and a water quality control method of combination of quicklime water and slow flowing water is optimized; for the tadpoles which are hatched in spring, conditions of illumination, water temperature, nutrition and the like are optimized, so as to shorten the growing period of the tadpoles; for the tadpoles which are hatched in autumn, corresponding conditions of illumination, water temperature, nutrition and the like are also optimized, so as to extend the growing period of the tadpoles; the growing development and metamorphosis development of the tadpoles are promoted, and diseases can be prevented, thereby increasing the survival rate and metamorphosis rate of the tadpoles.
Owner:江西明月山石蛙养殖股份有限公司

Method for inducing metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow

The invention relates to a method for inducing settlement and metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow. According to the method, firstly, dissolutionis promoted according to the weight volume ratio of 1:(45-60) (w / v) of kinoprene to dimethyl sulfoxide, and ethanol is added to prepare mutagenic agents containing 5-8mg / mL kinoprene. After a pond A and a pond B of a stepped culture pond are filled with clean sea water, the cypris larvae are transferred into the sea water in the pond A and the pond B of the stepped culture pond, and continuous micro-inflation is performed. Afterwards, a mutagenic agent dripping pipe is opened to continuously drip the mutagenic agents to the pond A. A water feeding pump of the stepped culture pond is started toinject water, and continuous water injection time is 4-6 days. 4-6 days after, water injection and dripping are stopped, cypris larva settlement bases are taken out, and then the cypris larvae can betransferred into a larva breeding stage. The method is simple in operation, less in energy consumption, low in cost, stable in function and remarkable in inducing effect, metamorphosis rate can reach80% or more, and the method is a good method for inducing settlement and metamorphosis of the capitulum mitella cypris larvae.
Owner:FUJIAN NORMAL UNIV

Rana spinosa tadpole feed and feeding method thereof

The invention discloses rana spinosa tadpole feed. The rana spinosa tadpole feed comprises artificial compound feed, vegetable pumpkin feed and mixed feed, wherein the mixed feed comprises the artificial compound feed and the vegetable pumpkin feed according to a weight ratio of 1: 1. After adoption of the rana spinosa tadpole feed, tadpoles accumulate more nutrition, and metamorphose into frogs with larger body shapes, so that the survival potential can be further improved and the survival rate can be increased; the tadpoles are omnivorous, and full compound feed meets food habits of the tadpoles and nutrients needed during growth, so that improvement on the disease resistance of the tadpoles is facilitated, and the tadpoles are high growth speed, long in total length and high in metamorphosis survival rate; through increase in the metamorphosis rate and the disease resistance, the growth cycle of the tadpoles is shortened to about 90 days, and the growth cycle is short. The rana spinosa tadpole feed and the feeding method thereof have the characteristics of capability of greatly increasing the survival rate, the total length and the metamorphosis survival rate of the tadpoles and great reduction in the feeding cost, and have the advantages of a short feeding cycle and strong disease resistance of the tadpoles.
Owner:GUIZHOU UNIV

Method for increasing pinctada maxima larva metamorphosis rate

The invention relates to a method for increasing pinctada maxima larva metamorphosis rate, and belongs to the technical field of shellfish culture. The method includes the specific steps: (1) preparing 70-100cm long palm ropes with the diameters of 2-3cm, soaking the palm ropes in 1% potassium permanganate solution for 10-12 hours, soaking the palm ropes in fresh water for 10-12 hours and cleaning residual potassium permanganate solution; (2) soaking the palm ropes in an outdoor cement pond for 1-2 days, continuously adding natural seawater with the quantity of 10-15L / min, and washing out sludge impurities attached to the palm ropes by filtered seawater after soaking; (3) placing 20-30 palm ropes into a nursery pond per m<3>; (4) transferring an attaching base to another nursery pond for culture after settlement and metamorphosis of larvae for 5-7 days; (5) placing the attaching base into an 80-mesh net bag and transferring fingerlings into a natural sea area for culture when the sizes of the fingerlings on the palm ropes reach 2-3mm. The proportion of eyebot larvae in the nursery pond is 50-70%, and the larva density is 0.5-1.0 piece / mL. The pinctada maxima larva metamorphosis rate and fingerling culture survival rate can be obviously increased, and the method is low in cost, simple and convenient.
Owner:GUANGDONG OCEAN UNIVERSITY

Method for increasing metamorphosis adhesion rate of eyespot larvae of pinctada martensi

The invention discloses a method for increasing the metamorphosis adhesion rate of eyespot larvae of pinctada martensi and belongs to the technical field of aquaculture. The method comprises the basicsteps of preparation of an adhesion water pond for the eyespot larvae of pinctada martensi, collection of the eyespot larvae, medicine feeding and young pinctada martensi breeding management; in theprocess of breeding young pinctada martensi, the eyespot larvae without metamorphosis adhesion in a young pinctada martensi breeding pond are collected and moved to a young pinctada martensi breedingpond with fully hung young pinctada martensi collection instruments for cultivation, antibiotics and vitamins are used for improving ingestion and digestion of the eyespot larvae, and the metamorphosis adhesion rate of the eyespot larvae of pinctada martensi is increased. By adopting the method, large-scale manual young pinctada martensi breeding of pinctada martensi is conducted, the metamorphosis adhesion rate of the larvae is high, the individual size of young pinctada martensi is even, the young pinctada martensi breeding yield of pinctada martensi can be increased, and the economic benefit of pinctada martensi can be improved; the method is suitable for large-scale manual young pinctada martensi breeding of pinctada martensi, easy to apply and popularize and obvious in social benefitand economic benefit.
Owner:广东尊鼎珍珠有限公司

Method for cultivating burying habitat type shellfish larvae

The invention discloses a method for cultivating burying habitat type shellfish larvae. The method comprises the following steps of after shellfish grows to D-shaped larvae, washing young shellfishesinto a cultivation basket, not adding a substrate into the cultivation basket, ensuring that the pond distribution density in the cultivation basket is 10ind/ml, and adhering a 300-mesh bolting silk net to the inner surface of the cultivation basket; putting the cultivation basket into a cultivation water body for cultivation, feeding bait organisms into the cultivation basket at regular time, andmaintaining the concentration of the bait organisms at 5*10<5>ind/ml; introducing oxygen into the cultivation water body to keep the cultivation water body rich in oxygen; and performing regular young shellfish washing and water changing. Compared with the prior art, the method has the following advantages that 1, the cultivation basket is arranged in the cultivation water body, the cultivation environment is improved, and the young shellfish growth speed and survival rate are increased; 2, the substrate does not need to be added, seawater permeates through the 300-mesh bolting-silk net, andthe influence of zooplankton on young shellfishes is reduced; and 3, the young shellfishes are basically not damaged by washing the young shellfishes and changing water, and the labor cost is greatlyreduced.
Owner:JIANGSU OCEAN UNIV

Breeding method capable of controlling aberration rate and body color abnormity of verasper variegatus seedlings

The invention relates to a breeding method capable of controlling the aberration rate and the body color abnormity of verasper variegatus seedlings and belongs to the field of aquatic seedling culture. The method comprises the following steps of: feeding reinforced rotifer to fish larvae of verasper variegatus, which are at the age of 5 to 6 days; when the verasper variegatus grows to the age of 19 to 21 days, simultaneously feeding artemia nauplii and reducing the feeding quantity of the rotifer until the verasper variegatus grows to the age of 34 to 36 days; and then starting to completely feed the artemia nauplii, wherein the artemia nauplii is reinforced by schizochytrium limacinum and the rotifer is reinforced by se-yeast after being reinforced by the schizochytrium limacinum. According to the invention, the Se-yeast is used as a reinforcer of the rotifer; content of selenium in a bait is improved; the fish larvae of verasper variegatus can be promoted to grow; the metamosphsis rate of the fish larvae of verasper variegatus is promoted; the aberration rate and the ratio of the body color abnormity are effectively reduced; survival rate is high; and the seedlings have high quality.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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