323 results about "Weight/volume ratio" patented technology

The invention relates to the technical field of batteries, and particularly provides a preparation method of a lithium-sulphur battery positive pole material with a sulfur-graphene composite structure. The preparation method comprises the steps of mixing sulphur powder and organic amine to prepare first dispersion liquid, wherein the weight-volume ratio of sulphur and the organic amine is 0.05-0.4 g:1 mL; dispersing graphene into an organic solvent to prepare second dispersion liquid, wherein the weight-volume ratio of the graphene and the organic solvent is 0.0005-0.02 g:1 mL; dripping the first dispersion liquid to the second dispersion liquid, and uniformly mixing to form third dispersion liquid; adding water or acid liquor to the third dispersion liquid, and continuing to stir so that a sulfur-graphene composite is separated out from the third dispersion liquid; carrying out solid-liquid separation on the third dispersion liquid, and drying an obtained first solid to obtain the lithium-sulphur battery positive pole material. The lithium-sulphur battery prepared through the method provided by the invention has the advantages of good coulomb efficiency and recycling; and the preparation method provided by the invention is simple, fast and low in consumption.

The invention belongs to the technical field of medicine preparation, and particularly relates to a freeze-drying protection system required for a nucleic acid amplification reagent and a preparationmethod of the freeze-drying protection system. The freeze-drying protection system comprises the nucleic acid amplification reagent and a freeze-drying protection additive, wherein the nucleic acid amplification reagent is a reagent used for LAMP reaction amplification, the freeze-drying protection additive is one or a compound of the following reagents: polyethylene glycol, mannitol, polyvinylpyrrolidone, glucan, trehalose, sucrose, bovine serum albumin, collagen, threonine and glycine, and the concentration of the weight-to-volume ratio of the freeze-drying protection additive and the amplification reaction reagent is 3% to 25%. The freeze-drying protection additive used in the invention has the advantages of small volume, short freeze-drying time, high efficiency and low energy consumption, and the freeze-drying protection system can be directly used for gene chip experiments, does not cause repeated freeze-thaw and waste of reagents, and can effectively ensure the activity of active substances in the freeze-drying process.

The invention relates to a test method for identifying cooking oil mixed with hogwash oil. The method comprises the following steps: precisely weighing a sample and placing the sample into a porcelain crucible, adding nitric acid, carbonizing on an adjustable electric hot plate with soft fire till no smoke comes out, and then transferring to a muffle and ashing to off-white at 550 DEG C, wherein the bulking value ratio of nitric acid to the sample to be tested is 1:1; dissolving ash content with 0.5mol/L nitric acid, transferring to a 25ml colorimetric tube, adjusting the constant volume of the colorimetric tube to a scale value, and testing the contents of main heavy metal elements by using an ICP-MS (inductively coupled plasma mass spectrometer). By comparing the tested results, whether the sample is mixed with hogwash oil can be identified. The ICP-MS method can test almost all elements in cooking oil and has higher sensitivity; meanwhile, by the method, samples of cooking oil with a low content of hogwash oil also can be exactly tested.

The invention provides a method for preparing fish meal protein peptide. The method comprises the following steps: (1) taking defatted marine fish meal as a raw material, and adding water for dissolving according to the weight-bulk ratio of material to liquid being (1: 5)-(12.5); (2) under the temperature of 115-130 DEG C and pressure of 0.75-1.75 kg/cm<2>, cooking for 20-30 minutes; cooling to obtain cooking liquid; (3) adjusting the pH value of the cooking liquid to 7.0-9.5; adding protease, controlling the mass concentration ratio of protease to substrate to be 0.5-2.5 percent, and carrying out enzymolysis under the temperature of 45-70 DEG C to obtain enzymatic hydrolysate; and (4) heating the enzymatic hydrolysate to the temperature of 90-95 DEG C, inactivating and drying. The fish meal protein peptide is high in small-molecule oligopeptide content and digestibility. The fish meal protein peptide as a feed additive is matched with feed to be eaten by animals, so that the bioavailability of fish meal protein can be improved, the animal immunity also can be improved, and the disease resistance is enhanced.

The invention discloses a method for preparing MnOOH nano rods, which prepares the MnOOH nano rods through a solvent thermal reaction of potassium permanganate and N,N-dimethylformamide , namely prepares the MnOOH nano rods through the following steps of: dissolving the potassium permanganate in the N,N-dimethylformamide according to a weight to volume ratio of 1:50 to 1:100 (g/ml); performing the solvent thermal reaction for 2 to 24 hours at the temperature of between 100 and 200 DEG C; then carrying out solid-liquid separation on a reaction product; washing the separated solid by using distilled water, absolute ethyl alcohol or acetone sequentially; and finally drying the washed solid at the temperature of between 30 and 100 DEG C to obtain the MnOOH nano rods. The invention aims to provide the method for preparing the MnOOH nano rods, which does not need a surface active agent or a template, has the advantages of high purity and uniform particle size distribution of the product and is suitable for industrial production. The method of the invention has simple process and simple and convenient operation and is suitable for industrial production.

The invention discloses a nucleic acid amplification reaction mixture. The nucleic acid amplification reaction mixed liquor is prepared by freeze drying and is in freeze-drying granular shape, and the water content is 0.1-3%; the nucleic acid amplification reaction mixed liquor at least contains necessary components for a nucleic acid amplification reaction and a freeze-drying protective agent; the freeze-drying protective agent is a mixture containing one or more substances from sucrose, trehalose, glucose, glucan, saccharosan, bovine serum albumin, collagen, polyvinyl pyrrolidone, polyethylene glycol and carboxymethylcellulose sodium, and the weight volume ratio concentration of the freeze-drying protective agent and the nucleic acid amplification reaction mixed liquor is 5%-20%. The nucleic acid amplification reaction mixture freeze-drying particles have compact and tight particle and smooth appearance, and fine apertures are contained in the particles and can be observed under microscopic amplification state; the freeze-drying particles can be stored at room temperature for more than one year with unchanged reaction activity, the biological reaction activity is not changed at 40-45 DEG C, and during usage, a reconstitution fluid is added for rapidly dissolving the particles and recovering the reaction activity.

The invention discloses a method for preparing antihypertensive peptides through enzymolysis of ground meat proteins of a tuna. The method comprises the steps of: (1) regarding the ground meat of the tuna as the raw material, mixing and homogenizing the raw material and a buffer solution according to a weight/volume ratio of 1 g: 3-5 ml, and then adjusting the pH value to 9.5-10 to obtain a mixed solution; (2) heating the mixed solution to 55-60 DEG C for pre-heating, and adding alcalase according to 1.0%-1.5% of the raw material for enzymolysis at an enzymolysis temperature of 55-60 DEG C for 4-6 h; and (3) performing enzyme deactivation on the obtained enzymolysis product to obtain an enzymolysis solution and then enabling the enzymolysis solution to sequentially suffer from ultrafiltration, desalination, freeze drying and chromatography to obtain the antihypertensive peptides. The method for preparing the antihypertensive peptides through the enzymolysis of the ground meat proteins of the tuna, disclosed by the invention, has the advantages of abundant source of the raw material, low price, simple preparation process, relatively strong activity of the prepared antihypertensive peptides; compared with a chemosynthetic ACE (Angiotensin Converting Enzyme) inhibitor, the ACEP (ACE Inhibitive Bioactive Peptide) prepared through enzymolysis of the ground meat of the tuna has the advantages of being safe and nontoxic, specific in antihypertensive efficacy, easy to digest and absorb and the like.

An oligo-proanthocyanidin with high ORAC value and its purification are disclosed. The products contain oligo-proanthocyanidin 45-65wt%, ORAC value is 15000 - 19000 mu-mol TE/g. The process is carried out by dissolving for proanthocyanidin crude product in solvent proportionally, adding into organic feed barrel of organic film ultra-filtration device, ultra-filtering for feed by organic film with molecular weight between 50000-1000000, concentrating and drying to obtain final product. It's convenient and efficient, has more film throughput and yield, and its costs low.

An aseptic powder of N(2)-L-alanyl-L-glutamine is prepared through adding N(2)-L-alanyl-L-glutamine to distilled water in aseptic condition, stirring, heating for dissolving, adding medical activated carbon, stirring, filtering for removing activated carbon, millipore filtering for removing bacteria, adding aseptic alcohol to educe out crystals, filtering, washing with cold alcohol, and vacuum drying. It has high optical and thermal stability.

The present invention belongs to the field of food fermentation technology, and is especially pure breed fermentation process for producing fermented soybean. The pure breed fermentation process includes the following steps: soaking soybean as the main material in water to water content of 45-55 %, and steaming at normal pressure or high pressure; inoculating Bacillus subtilis BBDC4 to KMB liquid culture medium; culture in shaking table at 150 rpm for 18 hr, and diluting the cultured liquid to obtain seed liquid with live bacteria number in 10<7> /ml; inoculating the seed liquid in 1-3 % to steamed soybean to produce leaven through setting at 30-37deg.c for 48-72 hr; adding salt, sugar and seasoning, and soaking in water for 12-24 hr; and loading into jar and setting at 45-55deg.c for 15-30 days to obtain fermented soybean in required flavor. The process has production period shorter than 34 days, pure yeast adopted to avoid harmful microbe invasion and ensured eating safety.

The invention discloses corn silk water. The water beverage is prepared by using corn silk and purified water as raw materials, and the weight-volume ratio of the corn silk to the purified water is 0.1-1kg: 100L, wherein in the corn silk water, the content of polysaccharide is 0.01 to 0.5g/L, the content of total saponin is 0.01 to 0.1g/L, and the content of total flavone is 0.01 to 0.1g/L. The invention also discloses a preparation method for the corn silk water. The method comprises the following steps of: putting the corn silk into a closed container, adding the pre-heated purified water into the container, and performing hot extraction and solid-liquid separation; and performing secondary filtration on the liquid part, then performing high-temperature sterilization, cooling the liquid to 90 DEG C, and performing hot filling, bottle pouring, spray cooling, lamp inspection and packing to obtain the corn silk water. The corn silk water is not added with any preservative, has the health-care functions of resisting oxidation, promoting urination, reducing pressure, reducing blood sugar, enhancing body immunity and the like, can replace drinking water to supplement water required by the human body every day, can be used as functional beverage for diabetes patients, and plays a role in prevention and health care.

The present invention relates to a method for preparing low impurity content, clear and transparent food-grade lecithin by utilizing inorganic ceramic membrane separation process, belonging to the field of separation and purification technology of food additive. Said method includes the following steps: using industrial hexane to dissolve feed-grade soybean lecithin to obtain its solution, using inorganic membrane to filter said solution at 40-55 deg.C, its filtering pressure is 0.1-0.4MPa, concentrating filtrate and evaporating so as to obtain the invented product.

The invention relates to a method to deoxidize the end coarse oil product and abstract gasoline and diesel oil with the coarse oil waste, and the facility and device used. The process is as following: (1)The material waste will be catalytic cracked at the temperature 50-480DEG C after some quartz sand,and grains of sand have been put in, the material waste is just the coarse oil waste. (2) Te gas from the cracking will be catalytic cracked further in the fixed bed and the oil steam can be gained. (3) The oil steam will be fractionated and the gasoline and diesel oil fraction will be collected respectively. (4) The gasoline and diesel oil fraction will be refined respectively. A lot of processes are set to remove the impurity in the whole technology, so the quality of product can be assured, and the facility is simple and the volume of it can be reduced, the technology has been predigested, and the production period has been reduced. The quality of the oil gained with the method is high, the transparence of the oil is also high, just like the pure water, the carbon content of the oil is low, and the oil belongs to gasoline without lead, the oil can reach the gasoline standard of the Chinese Standard of 93# gasoline, the yield of the oil is high, the quantity of the whole oil gained from the method is as much as the weight of 8-83% of the diesel oil waste.

The invention relates to a beverage, in particular to a compound ganoderma lucidum beverage and a preparation method thereof. The compound ganoderma lucidum beverage comprises the following raw materials according to weight-to-volume ratios: 1-5% of ganoderma lucidum sporocarp, 0.5-3% of radix puerariae and/or pueravia flower, 0.1-2% of hericium erinaceus, 0.1-2% of needle mushroom and the balance of purified water. The preparation method of the compound ganoderma lucidum beverage comprises the steps of 1) preparing a first extracting solution, 2) preparing a second extracting solution, 3) filtering the extracting solutions and scaling volumes, 4) performing filter membrane filtration, and 5) performing filling sterilization. According to the preparation method of the compound ganoderma lucidum beverage, an extraction method of the preparation method can ensure that specific fragrance and smell of ganoderma lucidum in a product are released out fully, and bitter substances of ganoderma lucidum and denatured protein with a peculiar smell can be prevented from being dissolved out. The compound ganoderma lucidum beverage prepared by the preparation method can fully exert characteristics and synergistic effects of multiple edible mushrooms and ganoderma lucidum, acts as a feature beverage, is sweet in taste and fragrant, can be drunk daily, can be used in banquets, and has the efficacies of improving immunity, strengthening health, protecting a liver, alleviating a hangover, nourishing a stomach and the like.

The invention relates to modified fish skin collagen and a preparation method. The preparation method comprises the following steps of: preparing 6.67 percent aqueous solution from the fish skin collagen in a weight volume ratio of g/ml, then adding glutamine transaminase in an amount which accounts for 2 percent of the weight of the fish skin collagen in the aqueous solution; after dissolving the glutamine transaminase fully, adding 2 percent solution of chitosan acetic acid in the volume ratio of 1-2:1; and homogenizing and performing vacuum degassing, and then heating the mixture at the temperature of between 40 and 50 DEG C for 30 minutes to obtain the modified fish skin collagen solution. The gel strength of the prepared modified fish skin collagen solution is 80 to 90g, modulus of elasticity is 550 to 740MPa at the temperature of 25 DEG C, and modulus of viscosity is 3,160 to 4,230MPa at the temperature of 25 DEG C. In the invention, the fish skin collagen is modified by combining the glutamine transaminase with the chitosan, so that the gel strength of the fish skin collagen solution is intensified, and the defect of low mechanical property is overcome. The modified fish skin collagen has a simple process and good modification effect and can be used for manufacturing packaging films in the fields of food, medicaments and cosmetic.

The invention provides a preparation method of fish meal protein peptide. The preparation method comprises the following steps: (1) taking degreased marine fish meal as the raw material, dissolving the fish meal in water according to a weight/volume ratio of 1:5-12.5; (2) boiling for 20 to 30 minutes at a temperature of 115 to 130 DEG C under a pressure of 0.75 to 1.75 kg/cm2, cooling so as to obtain a boiled liquid; (3) adjusting the pH value of the boiled liquid to 7.0-9.5, adding protease, controlling the mass concentration ratio of the protease to the substrate to be 0.5 to 2.5%, carrying out enzymolysis at a temperature of 45 to 70 DEG C so as to obtain enzymatic hydrolysate; (4) heating the enzymatic hydrolysate to 90 to 95 DEG C to inactivate the enzymes, adding active carbon, maintaining the temperature, continuously stirring at the same time so as to carry out decoloring and deodorization, then transporting the processed enzymatic hydrolysate into a plate-and-frame filter press to carry out filter pressing, condensing the clarified filtrate, and finally spray-drying so as to obtain the fish meal protein peptide. The prepared fish meal protein peptide is rich in nutrients, is easy to digest and absorb, and can strengthen the immunity. The nutrition value and biological utilization rate of low-market-value marine fishes are prominently improved. The protein peptide is suitable for preparation of various kinds of functional healthcare foods or nutrition foods.

The invention discloses corn stigma mineral water. The corn stigma mineral water is prepared from corn stigma and mineral water serving as raw materials, wherein the weight-to-volume ratio of the corn stigma to the mineral water is 0.1 to 1kg to 100L; the polysaccharide content of the corn stigma mineral water is 0.01 to 0.5g/L; the total saponin content is 0.01 to 0.1g/L; and the total flavone content is 0.01 to 0.1g/L. The invention also discloses a preparation method of the corn stigma mineral water; and the method comprises the following steps of: placing the corn stigma into a sealed container; adding preheated mineral water into the container; performing thermal extraction and solid-liquid separation; performing secondary filtration on the liquid part, sterilizing the liquid part at a high temperature and cooling the liquid part to 90 DEG C for thermal encapsulation; and pouring the liquid part into bottles, cooling the liquid part by spraying, performing visual inspection and packaging the bottles to obtain the corn stigma mineral water. The corn stigma mineral water does not have any preservative and has the health-care functions of antioxidation, diuresis, pressure lowering, blood sugar level regulation, enhancement of immunity of a body and the like, so that the corn stigma mineral water can replenish moisture required by human body every day in place of drinking water and can be used as a functional beverage for diabetic patients to play a part in prevention and health care.

The invention relates to the field of water-insoluble melanin development and utilization, in particular to a method for extracting water-insoluble melanin from animals, plants and microorganisms. The method includes the steps that firstly, dried, mildew-free and homogenized sunflower seed shells are selected and smashed, the smashed sunflower seed shells are mixed with 0.1-2.0 mol/L of NaOH according to the weight-to-volume ratio of 1:10-1:20, water bath is conducted on the mixture for 2.0-10.0 hours at the temperature of 40 DEG C to 80 DEG C, cooling and centrifuging are conducted, and a liquid melanin crude extract is obtained; the PH value is adjusted to range from 1.5 to 3.0, standing and sedimentation are conducted, and a melanin crude extract is obtained; impurities are removed through the steps of conducting boiling water bath hydrolysis for 2.0 hours to 6.0 hours in 5.0-7.0 mol/ L of HCl, conducting repeated washing through an organic solvent, conducting re-dissolving and the like, then vacuum free-drying is conducted, and dried pure melanin powder is obtained. The water-insoluble melanin extracted through the method has the advantages of being high in purity, high in yield and the like.

The present invention discloses a kind of oral azithromycin resin suspension and its preparation process, and aims at making the suspension possess good taste. The oral azithromycin resin suspension consists of in each 100 ml azithromycin 0.25-4.0 g, ion exchange resin 0.5-8.0 g and water for the rest. Its preparation process includes the following steps: mixing azithromycin, inorganic acid and/or organic acid and pure water; adding ion exchange resin to obtain mixed liquid; preparing suspension medium with suspending agent, surfactant and pure water; adding metal ion complexing agent, preservative, corrective and colorizing agent; adding the mixed liquid. The present invention is superior to available technology in that the oral azithromycin resin suspension has the bitter of azithromycin masked by the ion exchange resin and thus good taste.

A method for producing animal fodder by fermenting brewery mash by composite thallus is characterized in comprising: (1) drying the brewery mash, crushing, and sieving by a 20mu sieve; or crushing fresh brewery mash; (2) adjusting the material/liquid ratio by tap water, mixing well, sterilizing, and cooling for inoculation; (3)activating, and enrichment culturing the fermentation strain in total nutrient type culture medium for 24-30 hours, making preparation for inoculation; (4) placing the expanding culture thallus of neurospora crassa and lactic acid bacteria in aseptic technique condition, and inoculating on the actual production solid state fermentation culture medium according to the inoculation amount respectively in weight volume ratio of 6-8% and 8-10%; (5) placing the fermented brewery mash in the temperature of 70-80 degrees centigrade for drying, and obtaining the brewery mash fermented forage. The advantages of the invention are that: it improves the quality of brewery mash, and increases the utilization rate and digestion rate of forage; 2, reduces the investments of raw material and equipment of the microorganism fermented brewery mash forage, increases benefit; 3, reduces environmental pollution, saves a large amount of grain, and greatly reduces forage cost.

The invention relates to a method for extracting total DNA of microorganism in liquor Daqu, which is characterized in that, the liquor Daqu is pre-treated before extracting DNA, and the pre-treatment comprises the following steps: adding pre-treatment liquid into Daqu: the phosphoric acid buffer solution contains 0.05-0.2w/v% PVPP (polyvinylpolypyrrolidone) and 3-5w/v% tween-80 or tween-60, pH is8.0, wherein the weight volume ratio between Daqu and pre-treatment liquid is 3-8:25; shaking; executing ultrasound treatment; centrifuging at low speed after standing; centrifuging at a low speed after standing; removing supernatant, and adding the phosphoric acid buffer solution containing 0.05-0.2w/v% PVPP (polyvinylpolypyrrolidone) into the deposition; centrifuging at a high speed after scatter, removing supernatant for obtaining sample. The method provided by the invention is simple.

The invention discloses a field flue-cured tobacco regulator. By using water as a solvent, the field flue-cured tobacco regulator comprises the following raw materials in weight-volume ratio: 0.2-0.4 kg/L potassium nitrate, 0.2-0.4 kg/L potassium dihydrogen phosphate, 2.0-3.0 kg/L magnesium chloride, 5.0-6.0 g/L calcium nitrate, 0.5-1.5 g/L sodium borate, 0.2-0.6 kg/L potassium humate, 8-25 mg/L abscisic acid, 12-28 mg/L auxin, 0.3-1.8 mg/L brassinolide and a right amount of ethanol. The invention also discloses a preparation method and application of the regulator. The regulator disclosed by the invention can promote the field ripening enduring ability of the flue-cured tobacco, so that the tobacco has uniform maturity and yellowing, thereby enhancing the yield and quality of the flue-cured tobacco.

The invention belongs to the dearsenization method of Sargassum fusiforme, pertaining to foods processing field. Dry Sargassum fusiforme material is dipped in water for 2 hours, then cleaned and leached, and dipped in water solution of citric acid according to a weight volume ratio 0.05 percent to 0.5 percent for 2 to 8 hours. The treated Sargassum fusiforme is cleaned and dried under low temperature. The invention uses weak acid additive met with foods sanitary, quality and safety requirements to dearsenize Sargassum fusiforme, which is safe, reliable, and has simple technique, low cost, a dearsenization rate as high as 85 percent to 94 percent, and effectively cuts down the arsenium content of Sargassum fusiforme, without changing the original flavor and gloss and preserves the original nutrition components of the Sargassum fusiforme.