20 results about "Linear gradient" patented technology

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating and determining nafamostat mesylate SM2 and related impurities thereof. The method is a high performance liquid chromatography, and comprises the following steps: by taking octadecylsilane chemically bonded silica as a chromatographic column filler, a potassium hexafluorophosphate solution as a mobile phase A and acetonitrile as a mobile phase B, separating nafamostat mesylate SM2 and related impurities thereof through linear gradient elution; and detecting by using detection wavelengths of 200 nm to 240 nm and 250 nm to 280 nm to obtain a chromatogram, so that effective determination of the nafamostat mesylate SM2 and the related impurities thereof is realized. The method provided by the invention adopts common buffer salt as a mobile phase, and has the characteristics of short analysis time, strong specificity, high sensitivity and good reproducibility.

The provided method includes: determining an energy parameter and a co-volume parameter of a pure gas by a PR state equation, and determining a material parameter and a co-volume parameter of a mixed gas by a Waals one-fluid mixing rule and a combination rule; determining a Helmholtz free energy density and an equilibrium density of the pure gas by a Wertheim molecular association theory, and determining Helmholtz free energy and an equilibrium density of a (H2+N2) mixed gas with different components by a density mixing rule; determining an influence parameter of the pure gas by a gradient theory (GT), and determining an influence parameter of the mixed gas by the influence parameter of the pure gas and an interaction coefficient; simplifying a GT model; and obtaining the surface tension of the (H2+N2) / H2O system at different temperatures by combining the LGT model and the PR state equation.

The invention discloses a scanning type hyperspectral imaging device and method based on a linear gradient filter. The device sequentially comprises an imaging objective lens, a gradient filter assembly, a telecentric imaging lens group and a camera along the incident direction of a light path, during working, the one-dimensional linear displacement table drives the gradient filter assembly to perform linear scanning motion in the direction perpendicular to the optical axis, so that light in an imaging view field penetrates through band-pass filtering areas with different central wavelengths on the gradient filter in a time-sharing manner. The filtered monochromatic or narrow-band light is projected to a photosensitive target surface of the camera through the telecentric imaging lens group, and multiple frames of spectral images with different wavebands are continuously acquired. Collected sequence images are processed and reconstructed to form a hyperspectral three-dimensional data cube containing spatial information and spectral information. The system has the advantages of high imaging speed, high luminous flux, compact system structure, low cost and the like, and is suitable for hyperspectral data acquisition scenes with higher real-time requirements.

The invention discloses a pH value real-time regulation and stabilization method and system for online column separation, and belongs to the technical field of ion exchange chromatographic separation.The method comprises the steps that a proton disturbance signal is applied to a chromatographic column, an outflow response signal of the chromatographic column is detected, and a parameter map representing the dynamic buffer capacity characteristic of the chromatographic column is obtained; the parameter atlas and the target pH gradient are input into a physical transmission model based on convection, diffusion and adsorption coupling equations, and the physical transmission model outputs a predistortion pH gradient program used for compensating dynamic buffer capacity characteristics; according to a fluid proportion requirement which is defined by a pre-distortion pH gradient program and changes along with time; according to the method, dynamic buffer capacity characteristics can be obtained through a proton disturbance test, a pre-distortion pH gradient program is constructed, and closed-loop iterative optimization is combined, so that interference of the dynamic buffer capacity of the chromatographic column on proton transfer is effectively counteracted, a post-column effluent pH curve is accurately matched with a target linear gradient, and the problems of pH nonlinear distortion and a platform area are eliminated.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to an HPLC (High Performance Liquid Chromatography) method for separating and determining nafamostat mesylate X1 and impurities thereof. The impurities comprise an impurity NAA-SM2, an impurity NAA-SM2a, an impurity NAA-SM2f, an impurity NAA-X1d, an impurity NAA-X1b, an impurity NAA-X1e, an impurity NAA-X1f and isopropyl p-aminobenzoate. According to the method, octadecyl silane bonded silica gel is adopted as a chromatographic column filler, a potassium hexafluorophosphate solution is adopted as a mobile phase A, acetonitrile is adopted as a mobile phase B, and nafamostat mesylate X1 and impurities thereof are separated through linear gradient elution; and then calculating the content of each impurity by adopting an external standard method and / or a principal component self-contrast method multiplied by a correction factor according to a chromatogram. The method has the characteristics of good separation degree, good durability, high sensitivity and good reproducibility.

This invention belongs to the field of pharmaceutical analysis technology, specifically relating to a method for separating and detecting rifabutin and its impurities. The impurities include impurity B, impurity D, impurity E, impurity F, impurity G, impurity I, impurity J, impurity K, and impurity Z. 3b Impurity Z 3c Any one or more of the following. This method is a reversed-phase high-performance liquid chromatography (RP-HPLC) method. The chromatographic column uses octadecyl-bonded silica gel as the packing material, and a linear gradient elution is performed using mobile phases A and B. The chromatogram is obtained by detection using a detector. Mobile phase A is a mixture of phosphate buffer solution and organic solvent, and mobile phase B is a mixture of acid aqueous solution and organic solvent. This method can completely separate and detect rifabutin and its 10 impurities within 60 minutes, and features good resolution, robustness, high sensitivity, and good reproducibility.

The application provides an image processing method based on a feathering mask and related components, and the technical scheme points are as follows: obtaining an inferred image sub-block and a position thereof; initializing a full-one mask for each sub-block; respectively calculating linear gradient weight maps in horizontal and vertical directions; multiplying the three to generate a target feathering mask with four-side gradient attenuation; and finally, weighting each sub-block and the corresponding mask, and then adding to a canvas according to the position to obtain a fusion image. Compared with the prior art, the application makes the corresponding pixel points of adjacent sub-blocks in the overlapping area satisfy a weight complementary relationship, reduces the condition that a traditional simple average or fixed weight fusion produces obvious joint lines at the splicing position, and thus improves the visual continuity of the fusion image.

The application relates to a coating equipment and a linear gradient filter film thickness uniformity correction method, and solves the problem that the film thickness uniformity of a linear gradient filter prepared by using an existing deposition method of electron beam evaporation cannot be corrected. A revolution correction plate is arranged below a workpiece disc, the revolution correction plate rotates with the workpiece disc around the center of a rotating disc, the workpiece disc has self-rotation motion, the film thickness distribution of Ta2O5 and SiO2 is corrected to the middle linearity by the revolution correction plate, at this time, the film thickness distribution of Ta2O5 and SiO2 after being corrected by the revolution correction plate has not reached the final target, and the difference from the final target is respectively corrected for the second time by a first fixed correction plate and a second fixed correction plate, so that the film thickness distribution of Ta2O5 and SiO2 finally reaches the final target, and the film thickness distribution of Ta2O5 and SiO2 is corrected to the film thickness distribution state required by the linear gradient filter by the combination of the revolution correction plate and the fixed correction plate.

The present disclosure is directed to methods of purifying a dulaglutide composition. For example, the methods comprise loading the dulaglutide composition in-line with a buffer B solution onto a hydrophobic interaction chromatography (HIC) column in a downflow direction, washing the HIC column with a buffer mixture of a buffer A solution and the buffer B solution in the downflow direction, and eluting dulaglutide from the HIC column. A linear gradient of a percentage of the buffer A and buffer B solutions is used for eluting dulaglutide, which is then followed by an isocratic hold based on a UV absorbance reading of an eluate. The methods further comprise maintaining the isocratic hold until a front side cut is made, and then dropping the buffer B solution for dulaglutide to elute from the HIC column.

The invention relates to a separation and quality control detection method for related substances in deoxycholic acid, which adopts a high performance liquid chromatography method, a chromatographic column takes octadecyl bonded silica gel as a filler, 0.010 mol / L dipotassium phosphate solution as a mobile phase A and acetonitrile as a mobile phase B, linear gradient elution is carried out, and the related substances are subjected to separation and quality control detection. Wherein the related substance is pyridine. The quality control detection method for related substances in deoxycholic acid provided by the invention comprises the following steps: preparing a pyridine reference substance solution with a certain concentration, determining the peak area of pyridine in a reference substance and a test solution by using a high performance liquid chromatography, and calculating the content of pyridine by using an external standard method. Therefore, the content of pyridine in deoxycholic acid can be accurately and quantitatively determined.

The invention belongs to the technical field of chemical analysis, and particularly relates to a method for separating and determining related substances in a rupatadine fumarate intermediate Z2. The related substances comprise any one or more of an impurity SM1, an impurity Z1, an impurity Z2a and an enantiomer thereof, an impurity Z2b and an enantiomer thereof, and an impurity Z2e and an enantiomer thereof. According to the self-built high performance liquid chromatography, octadecyl silane bonded silica gel is adopted as a chromatographic column filler, a phosphate solution is adopted as a mobile phase A, acetonitrile is adopted as a mobile phase B, and related substances in the rupatadine fumarate intermediate Z2 are separated through linear gradient elution; then detecting in a detector to obtain a chromatogram; and calculating the content of each impurity by adopting a correction factor-added principal component self-contrast method. The analysis method has the characteristics of short separation time, high sensitivity, strong specificity, good reproducibility, strong durability and simplicity and feasibility in operation.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating, detecting and determining an initial raw material SM3 and impurities thereof of ibrutinib. The impurities comprise one or more of propionyl chloride, 3-chloropropionyl chloride and benzoyl chloride. A mobile phase is a phosphate buffer solution as a mobile phase A, acetonitrile is a mobile phase B, a chromatographic column adopts octadecylsilane chemically bonded silica as a filler, and linear gradient elution of high performance liquid chromatography is carried out after pre-column derivatization. And then, according to a chromatogram, an external standard method is adopted to calculate the impurities according to a peak area. The method has the characteristics of good separation degree, good durability, high sensitivity and good reproducibility.

This invention discloses a method and system for real-time pH control and stabilization in online column separation, belonging to the field of ion exchange chromatography separation technology. The method includes: applying a proton perturbation signal to the chromatographic column and detecting its elution response signal to obtain a parameter spectrum characterizing the dynamic buffer capacity of the chromatographic column; inputting the parameter spectrum and the target pH gradient into a physical transport model based on convection, diffusion, and adsorption coupling equations; the physical transport model outputs a pre-distorted pH gradient program to compensate for the dynamic buffer capacity characteristics; and based on the fluid ratio requirements defined by the pre-distorted pH gradient program and varying over time. This allows for the acquisition of dynamic buffer capacity characteristics through proton perturbation testing and the construction of a pre-distorted pH gradient program. Combined with closed-loop iterative optimization, this effectively counteracts the interference of the chromatographic column's dynamic buffer capacity on proton transport, ensuring that the pH curve of the post-column elution accurately matches the target linear gradient and eliminating pH nonlinear distortion and plateau problems.

The application provides a multispectral coating method of a linear gradient filter and the linear gradient filter, and the method comprises the following steps: S1, placing optical glass into a cleaning agent for ultrasonic cleaning to obtain a substrate of the linear gradient filter to be coated; S2, placing the substrate into a reaction cavity of a radio frequency magnetron, closing the reaction cavity, and setting environmental parameters for multispectral coating; S3, under the environmental parameters, using a double-target radio frequency magnetron to sputter and deposit target materials on the surface of the substrate, wherein SiO2 and Si are used as the target materials of the double-target, and the power interval of the radio frequency magnetron is adjusted to switch the sputtering and deposition of the target materials of different target positions alternately; and S4, closing the radio frequency magnetron, keeping the reaction cavity airtight, naturally cooling the reaction cavity to a preset cooling temperature, opening the reaction cavity, and taking out the substrate with the coating as the linear gradient filter. The application has a simple process, good infrared spectral performance of the filter product, and is suitable for various scenes.

This invention belongs to the field of pharmaceutical analysis technology, specifically relating to a method for separating and determining SM1, the starting material of utpatinib, and its impurities. The impurities include SM1. 1a SM 1b SM 1d SM 1f SM 1h SM 1i SM 1j SM 1m SM 1n and SM 1o One or more of the following. This invention provides a self-developed high-performance liquid chromatography (HPLC) method for the separation and determination of utpatinib starting material SM1 and its impurities, comprising using phosphoric acid solution as mobile phase A and an organic solvent as mobile phase B; using octadecylsilane-pentafluorophenyl alternating bonded silica gel as the packing material, performing linear gradient elution, and then entering a detector for detection; based on the measured chromatogram, the content of each impurity is calculated using the principal component self-comparison method with correction factors and / or the limit method. This method features good resolution, robustness, high sensitivity, and good reproducibility.

This invention discloses an epitaxial method for InGaAs layered linear gradient variation buffer layers based on molecular beam epitaxy, comprising the following steps: S1: Determine the total variation range of the specific In composition and the total thickness of the linear variation of In, and calculate the retreat composition of the retreat layer based on the variation rate of the In composition; S2: Design the InGaAs linear gradient buffer layer in layers and determine the thickness of the entire layer; S3: Determine the value of the In composition after actual epitaxy, and adjust the In composition and thickness in step S2 according to the value after actual epitaxy; S4: Determine the growth rate of each gradient layer of InGaAs; S5: Epitaxially construct the InGaAs layered linear gradient variation buffer layer based on the layer thickness and the growth rate of each gradient layer of InGaAs. This invention realizes the InGaAs layered linear gradient variation buffer layer. x Ga 1‑x As linear gradient buffer layer, the linear change of In composition in As reduces the influence of source furnace temperature changes on the uncertainty of In and Ga growth rate changes.

The invention belongs to the technical field of analytical chemistry, and particularly relates to an HPLC (High Performance Liquid Chromatography) method for separating and determining rupatadine fumarate SM2 and impurities thereof. The method comprises the following steps: by taking octadecylsilane chemically bonded silica as a chromatographic column filler, a phosphate solution as a mobile phase A and methanol as a mobile phase B, separating rupatadine fumarate SM2 and related impurities thereof through linear gradient elution, and detecting by adopting a detector to obtain a chromatogram; and each impurity is quantified according to a correction factor-added principal component self-contrast method and a limit method. The method realizes effective detection of 15 impurities, namely SM2f, SM2j, SM2k, SM2a, SM2b / SM2d, SM2c, SM2e, SM2g, SM2h, SM2i, SM2l, SM2m, SM2n and SM2o, in rupatadine fumarate SM2, and has the characteristics of short separation time, strong specificity, high sensitivity, good reproducibility and strong durability.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a method for separating and determining Enzalocamide Z3 and genotoxic impurities thereof. The impurities comprise one or more of an impurity Xd, an impurity Xg and an impurity Xh. The method comprises the following steps: carrying out linear gradient elution of high performance liquid chromatography by taking octadecyl silane bonded silica gel as a chromatographic column filler, taking a trifluoroacetic acid solution as a mobile phase A and taking acetonitrile as a mobile phase B; and entering a detector for detection. And judging whether the content of each impurity in the sample is qualified or not by adopting a limit method according to the chromatogram. The method disclosed by the invention has the characteristics of good separation degree, good durability, high sensitivity and good reproducibility.

The application relates to the technical field of linear gradient optical filter preparation, and provides an etching device, method, equipment, medium and program product for an optical device, the etching device comprising: an ion source for providing an ion beam for etching; a vacuum chamber for providing a vacuum environment; a workbench arranged in the vacuum chamber; and a control assembly connected to the ion source and the workbench respectively; the workbench comprises multiple groups of processing areas for respectively arranging multiple groups of optical materials; and the control assembly controls the workbench to move relative to the ion source, so that the ion beam is scanned relative to each group of processing areas according to a preset speed distribution and a preset acceleration distribution, and the gradient etching of each group of optical materials is realized. The embodiments of the application can be applied to the processing of large-size optical devices with a gradient structure, have high processing precision and processing efficiency, and are suitable for batch production of the optical devices.