1360 results about "Buffer solution" patented technology

The utility model discloses a microfluidic system for target object pairing, which comprises a first flow channel, a second flow channel, an oil liquid flow channel, a buffer liquid flow channel, a matching flow channel and a pairing micro valve, a first micro valve, a second micro valve and a buffer liquid micro valve are arranged, the matching flow channel is communicated to the oil liquid flow channel, and the first flow channel, the second flow channel and the buffer liquid flow channel are all communicated to the matching flow channel; when a single first target object and a single second target object exist in the matching runner, the first micro valve and the second micro valve are in a closed state, and the matching micro valve and the buffer solution micro valve are in an open state; and the buffer liquid in the buffer liquid flow channel feeds the single first target object and the single second target object in the flow channel into the oil liquid in the oil liquid flow channel to form liquid drops. According to the utility model, other first target objects and / or other second target objects can be completely prevented from entering liquid drops, so that the success rate of packaging is improved, and the cell loss caused by multiple packaging is reduced.

The invention belongs to the field of synthesis of nano materials, and particularly relates to a core-shell structure gold nano star and a preparation method and application thereof, and the core-shell structure gold nano star comprises a first gold nano star containing a metal sulfide shell layer and a second gold nano star containing a metal oxide shell layer. The preparation method comprises the following steps: firstly preparing a gold nano star core solution, and then respectively adding different precursors to prepare two core-shell structure gold nano stars. The method has the advantages of simple synthesis conditions and non-toxic reagents. The core-shell structure gold nanostars are used for preparing a detection solution based on a buffer solution, a color developing agent and an oxidizing agent, the detection solution reacts with antioxidants, a data matrix is constructed by measuring absorbance changes and is analyzed, single, binary and ternary combinations of various antioxidants such as ascorbic acid and glutathione can be accurately distinguished, and the detection accuracy is improved. The application prospect in the fields of biomolecules and food detection is good.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention relates to the technical field of pharmaceutical preparations, in particular to a multiple nano emulsion adjuvant and a preparation method thereof. The multiple nano emulsion adjuvant is prepared from 2.73 to 6.37 g of an oil phase matrix based on 100 g of a raw material system, 0.10 to 0.25 g of a sterol compound; 0.212 to 0.424 g of a nonionic surfactant; 0.00050 to 0.00200 g of a triterpenoid saponin type active adjuvant; 0.010 to 0.020 g of an anionic polymer modifier; 0.005 to 0.015 g of a cationic polymer modifier; 70-90 g of a buffer solution; the total mass of the raw materials is 100 g. The nano emulsion adjuvant prepared by the invention can be stored for 6 months at 2-8 DEG C under a low sterol condition, the particle size change does not exceed + 5%, no crystallization or layering is generated, and the stability of the active adjuvant is remarkably improved.

The invention provides a preparation method and application of red nano-selenium particles, and relates to the technical field of nano-medicines.The preparation method comprises the steps that sodium selenite is dissolved in ultrapure water, silicate microspheres containing copper, zinc and aluminum are added, the pH value is 4.8, 1.5 M of ascorbic acid solution is dropwise added while stirring, the temperature is increased till solid ascorbic acid is added, the pH value is adjusted through NaOH, and nano-selenium suspension liquid is obtained through stirring reaction; the preparation method comprises the following steps: mixing and stirring a nano-selenium suspension, a sodium alginate sulfydryl graphene oxide mixture and a chitosan oligosaccharide citric acid buffer solution, centrifugally washing, and freeze-drying to obtain red nano-selenium particles, and preparing the copper-zinc-containing aluminosilicate microspheres through mesoporous template calcination and copper-zinc co-doping. The red nano-selenium particles prepared by the method have a core-shell structure, uniform particle size, high oxidation resistance and remarkable interface bonding strength.

The invention relates to the technical field of sewage treatment, in particular to an electrostatic spinning PVDF nanofiber membrane for sewage treatment and a preparation method and application of the electrostatic spinning PVDF nanofiber membrane. The preparation method comprises the following steps: dissolving PVDF (Polyvinylidene Fluoride) particles in a mixed solvent of DMF (Dimethyl Formamide) and acetone, adding nano TiO2 particles subjected to APTES (Aminopropyltriethoxysilane) surface modification, and adding PVP (Polyvinyl Pyrrolidone) into the solution to obtain a uniform spinning solution; spinning by adopting electrostatic spinning equipment to obtain a PVDF / TiO2 / PVP nanofiber membrane; the nanofiber membrane is soaked in a sodium hydroxide solution, then the membrane is soaked in a Tris-HCl buffer solution containing dopamine, and the final electrostatic spinning PVDF nanofiber membrane is obtained. The average diameter of nanofibers of the nanofiber membrane is 200-300nm, the porosity of the membrane is 70-80%, and the water contact angle is less than 60 degrees; when the nanofiber membrane is used for treating sewage containing organic pollutants and heavy metal ions, the removal rate of the nanofiber membrane on methyl orange can reach 95% or above; the rejection rate of copper ions can reach 90% or above; the removal efficiency of organic pollutants and heavy metal ions is obviously improved.

The invention relates to a metallized microbubble and a preparation method thereof, a construction method of a multifunctional composite probe, and a rapid bacterium capturing and sterilizing method, and belongs to the technical field of functional material preparation. The method aims at solving the technical problems that in the prior art, a metal coating on the surface of the hollow glass microbubble is not uniform, combination is not firm, and stability is poor. According to the technical scheme, the method is characterized by comprising the following steps: mixing surface-activated hollow glass microbubbles with a polyethyleneimine solution for reaction, and washing and drying to obtain polyethyleneimine-coated hollow glass microbubbles; then reacting with a single-stranded DNA buffer solution to obtain an intermediate product loaded with DNA; then introducing a silver nitrate solution to load silver ions on the surface of the compound; and finally, reducing through a sodium borohydride solution to prepare the metallized microbubbles. The metalized microbubbles prepared by the method can be used for constructing a multifunctional composite probe to realize efficient capture and synergistic antibiosis of targets such as bacteria.

The invention relates to the technical field of agricultural fertilizer preparation, and discloses a preparation method of a water-soluble fertilizer rich in various active amino acids, which comprises the following steps: S1, preparing an amino acid solution: dissolving various free amino acids or polypeptides in a buffer solution to form an amino acid precursor liquid; s2, constructing a nutrition core layer: adding one or more types of soluble trace element salts and a gelation auxiliary agent into the amino acid precursor liquid to form gelatinous particles; s3, forming an intermediate response layer: coating the core particles with chitosan and phospholipid to form a bimolecular membrane structure. The stability of the particle structure is improved through low-temperature crosslinking and bi-component gelation, and quick response and continuous controlled release in a weak acid environment are realized by combining an intermediate response membrane and an outer controlled release shell layer; by adopting a structure-function integrated design, the suspension property and the dispersity are remarkably improved, and the multi-crop and multi-period precise fertilization requirements are met.

The invention provides a separation and purification method of urokinase, and relates to the technical field of separation and extraction. The separation and purification method comprises the following steps: S1, regulating the pH value of male urine to 8.5, standing, and taking supernate; s2, mixing the supernate with an adsorption material, and eluting with an eluent to obtain a urokinase crude product solution; the adsorption material is a core-shell silica gel polymer, Fe3O4atSiO2-NH2 is taken as a core, and polystyrene is taken as a shell; the eluent is a sodium citrate buffer solution with the concentration of 0.5 to 0.15 M, acetonitrile with the concentration of 1 to 5 weight percent and EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 1.5 mM; and S3, carrying out anion exchange column chromatography on the urokinase crude product solution, eluting with an eluent, and precipitating to obtain the urokinase. The urokinase prepared by the method has the advantages of high polymer urokinase content, high recovery rate and high activity yield.

The invention discloses a reaction buffer solution for improving endotoxin detection sensitivity of a recombinant tachypleus amebocyte lysate, a kit containing the reaction buffer solution and application of the reaction buffer solution. The reaction buffer solution comprises the following components: a Tris-HCl buffer solution, magnesium ions, sodium chloride, cane sugar, ethylene glycol diethyl ether diamine tetraacetic acid, a nonionic surfactant and cyclodextrin or a derivative thereof. The invention also discloses a preparation method of the reaction buffer solution and a kit containing the reaction buffer solution. When the reaction buffer solution or the kit is used for detecting the endotoxin of the recombinant tachypleus amebocyte lysate, the sensitivity of the recombinant tachypleus amebocyte lysate for detecting the endotoxin can be improved by one order of magnitude, the lowest detection limit can reach 0.0005 EU / ml, the reaction buffer solution or the kit has strong anti-interference capability and short reaction time, and the detection efficiency is greatly improved.

The invention discloses a preparation method of a high-stability manual buffer solution, which comprises the following steps of: introducing two types of slow-release porous particle structures, namely a first type of particles loaded with acidic or alkaline components and a second type of particles loaded with silver nanoparticles, so that continuous slow release and dynamic adjustment of the components in the buffer solution are realized; the pH drift and the aging of a buffer system are effectively delayed; meanwhile, slow release of the silver nanoparticles endows the buffer solution with lasting antibacterial protection, microbial growth is remarkably inhibited, and due to the structural design, the long-term pH stability and storage safety of the buffer solution are remarkably improved, the clarity and physicochemical properties of the buffer solution are guaranteed, and the stability of the buffer solution is improved. The method greatly expands the application range in the fields of high-requirement biological experiments, medical detection, long-term sample storage and the like, the particle addition amount and the component proportion can be flexibly adjusted according to actual requirements, the method adapts to different pH ranges and application scenes, and various experiment requirements of protein purification, long-term sample storage, high-sensitivity analysis and detection are met.

The application discloses a preparation method of a hydrophilic oil-water separation anti-pollution membrane and the anti-pollution membrane and application thereof, and prepares MNRs material; the MNRs material is dispersed in dimethylacetamide, is uniformly stirred, polyvinylpyrrolidone is added, and stirring is continued, and a first mixture is obtained through ultrasonic treatment; the first mixture is mixed with polyvinylidene fluoride to obtain a second mixture, and the second mixture is stirred; the treated second mixture is dip-coated on a carrier, the dip-coated carrier is treated in deionized water to obtain an ultrafiltration membrane; the ultrafiltration membrane is soaked in a tea polyphenol solution with a Tris-HCl buffer solution, and is shaken to obtain the hydrophilic oil-water separation anti-pollution membrane. The hydrophilic oil-water separation anti-pollution membrane has excellent separation efficiency and flux recovery rate for four different oil-water mixtures of kerosene, gasoline, edible oil and n-hexane. The preparation process of the oil-water separation membrane is simple, the cost is low, and the reaction condition is mild; when the oil-water separation membrane is applied to organic wastewater separation, the oil-water separation membrane has the advantages of high efficiency, good removal performance and good recycling performance.

The invention relates to the technical field of bone tissue materials, and discloses a metal-doped biomimetic mineralized dECM cryogel and a preparation method and application thereof.The preparation method of the cryogel comprises the steps that firstly, hyaluronic acid and methacrylic anhydride are mixed in a solvent, the pH value is adjusted, a reaction is conducted, and methacrylic acid hyaluronic acid is obtained; then under the condition of a photoinitiator, mixing the methacrylic acid hyaluronic acid with an acellular extracellular matrix, and performing freezing and crosslinking treatment to obtain the cryogel, and finally, soaking the cryogel in a mineralization solution containing Ca < 2 + > and / or Mg < 2 + >, adding a buffer solution containing phosphite ions, incubating, and washing to obtain the crystal gel. The biomimetic mineralized dECM cryogel prepared by the method disclosed by the invention is good in ion release stability, can significantly improve the migration ability of HUVECs, contributes to promoting proliferation of rMSCs and improving the osteogenic differentiation potential of the rMSCs, and can be used for regeneration and repair of bone defects.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic buffer solution, mixing hydroxytyrosol and vitamin C ethyl ether, and carrying out a dehydration condensation reaction to prepare the modified hydroxytyrosol. According to the provided preparation method of the modified hydroxytyrosol, the vitamin C ethyl ether is adopted to modify the hydroxytyrosol, the preparation method is simple and easy to operate, and the prepared modified hydroxytyrosol has high oxidation resistance and inflammation resistance.

The present invention relates to a monovalent selective cation exchange membrane and a preparation method thereof, belonging to the technical field of cation exchange membranes. The specific steps are: weighing DA and ammonium persulfate and adding them to a buffer solution to obtain a PDA coating solution, pouring the PDA coating solution on the surface of a base membrane for soaking, depositing a PDA coating on the surface of the activated base membrane to obtain membrane I; dissolving zirconium tetrachloride and 2-aminoterephthalic acid in DMF, then solvent-thermally synthesizing an amino-functionalized MOF (UIO-66-NH2) under acidic conditions, weighing PEI and MOF (UIO-66-NH2) powders and adding them to a buffer solution, then pouring them on the surface of membrane I, depositing a PEI-MOF composite coating to obtain membrane II, and air-drying membrane II to obtain a monovalent selective cation exchange membrane. It can be seen that the present invention can prepare a low-cost, highly selective, and stable-performance monovalent selective cation exchange membrane by modifying the cation exchange membrane through a simple and easy-to-operate method.

The invention belongs to the technical field of medical gel, and particularly relates to modified hydrogel for skin wound healing and a preparation method thereof.The modified hydrogel is prepared from, by weight, 2-5 parts of drug-loaded GDVLNs, 1-2 parts of chitosan, 0.6-1.5 parts of antioxidant, 0.2 part of LAP, 1.5 parts of MA, 18 parts of gel matrix and 100 parts of buffer solution, the drug-loaded GDVLNs prepared from the traditional Chinese medicine active ingredients and the ginseng vesicles have the functions of resisting oxidative stress, promoting angiogenesis, promoting cell proliferation and the like, and the MA modified hydrogel system can realize slow release and controlled release of the drug-loaded GDVLNs and play a long-acting healing promoting role.

The invention relates to the technical field of detection kits, in particular to an HbA1c detection kit and a preparation method thereof, and the kit comprises latex microsphere freeze-dried powder DP-1, HbA1c monoclonal antibody freeze-dried powder DP-2, buffer solution dry powder H-1 and buffer solution dry powder H-2. A composite preparation scheme of combining a microsphere and antibody freeze-drying process and a buffer reagent drying process is adopted, on one hand, the traditional biochemical reagent freeze-drying process is greatly simplified through process optimization, and the production time consumption and the cost investment of the freeze-drying link are effectively reduced; and on the other hand, the cold chain dependence in the product storage and transportation process is remarkably reduced due to the dry reagent form, and the overall transportation cost and loss risk are greatly reduced.

The invention discloses a method for separating an egg white polypeptide chain by adopting a coupling membrane, and particularly relates to the field of egg white polypeptide chains, the method comprises the following steps: S1, mixing fresh egg white with a buffer solution, adjusting the initial acid-base environment and initial temperature of a system, and measuring key basic parameters after dispersion treatment; s3, introducing the pretreated egg white liquid into a first-stage membrane assembly, monitoring separation process parameters, obtaining interception efficiency, dynamically adjusting operation parameters, and enabling permeate liquid to enter a second-stage membrane assembly; s7, monitoring system environment parameters and polypeptide activity, obtaining an activity retention rate, dynamically adjusting supply parameters, and maintaining the polypeptide activity; s8, classifying and collecting products according to molecular weight intervals, evaluating comprehensive separation efficiency, completing separation and verifying product indexes; by constructing an ultrafiltration membrane-nanofiltration membrane-reverse osmosis membrane three-stage coupling separation system and combining a dynamic parameter adjustment mechanism based on interception efficiency, separation selectivity and refined purity, the purity and separation efficiency of a polypeptide product are remarkably improved.

The invention discloses a modified microfiltration membrane for enriching biological particles and a preparation method and application thereof, and belongs to the technical field of biological separation.The preparation method of the modified microfiltration membrane comprises the following steps that a mixed cellulose ester microfiltration membrane is provided and subjected to alkali liquor activating treatment; placing the activated membrane in a weakly alkaline buffer solution for balancing; the preparation method comprises the following steps: in a weakly alkaline buffer solution, firstly contacting the surface of the membrane with a tannic acid solution to carry out a first reaction, and then directly adding a polyethyleneimine solution to carry out a second reaction under the condition of not replacing a system, so as to form a phenol-amine cross-linked network on the surface of the membrane in situ; sodium cyanoborohydride is continuously added for reduction stabilization treatment, and the modified microporous filter membrane is obtained. The modified microporous filter membrane prepared by modifying the microporous filter membrane with tannic acid and polyethyleneimine can realize one-step synchronous enrichment of all negatively charged targets, and the problems of target loss, efficiency loss and the like caused by a multi-step process are avoided from the source.

The invention belongs to the technical field of water treatment, and particularly relates to a preparation method and application of a PDA / CS composite adsorbent loaded with a bimetallic system, and the preparation method comprises the following steps: 1) dissolving dopamine in a Tris buffer solution to prepare a dopamine prepolymer solution; then adding FeCl3, and carrying out constant-temperature water bath; centrifuging the obtained black suspension, performing suction filtration, washing, collecting a filter cake, and performing freeze drying to obtain PDA microspheres; 2) dispersing PDA microspheres in deionized water, and carrying out ultrasonic treatment until the PDA microspheres are uniformly dispersed; 3) dissolving chitosan in an acetic acid solution, and adding CuCl2 to prepare a mixed solution; the suspension obtained in the step 2 is dropwise added into a mixed solution for constant-temperature water bath, the pH of the mixed solution is adjusted through a NaOH solution, glutaraldehyde is added for constant-temperature water bath after constant-temperature water bath, and 4, the obtained suspension is subjected to centrifugation, suction filtration, washing, filter cake collection and freeze drying.

The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.

The invention belongs to the technical field of medicines, and particularly relates to a hydrogel dressing for promoting healing of diabetic wounds as well as a preparation method and application of the hydrogel dressing. According to the hydrogel dressing for promoting healing of the diabetic wound, glycyrrhizic acid and S-allyl-L-cysteine are used as raw materials, a buffer solution is used as a dispersion medium, and the glycyrrhizic acid and S-allyl-L-cysteine are compounded and self-assembled by controlling the mass ratio of the raw materials, the reaction temperature, the reaction time and the pH value. The hydrogel shows tissue-like viscoelasticity characteristics, can adaptively deform along with wound movement, provides continuous protection for chronic wounds under dynamic physiological conditions, and has flexibility of adapting to dynamic requirements in different healing stages.

A lipid nanoparticle formulation having remarkably improved stability and a preparation method therefor. The lipid nanoparticle formulation comprises a pharmaceutically acceptable buffer solution, lipid nanoparticles and a nucleic acid encapsulated in the lipid nanoparticles.

The invention relates to a chemical aging device comprising an enclosure inside which are installed a plurality of test receptacles (16), each test receptacle containing a certain amount of aqueous solution (18) and a membrane sample (12), each test receptacle (16) being equipped with a gas and vapour evacuation duct (22) connected to its internal volume, the various evacuation ducts (22) of the various test receptacles (16) being connected to a gas and vapour extraction duct (24), this extraction duct (24) passing through a wall of the closed enclosure, this extraction duct (24) being connected to a container flask (26), situated outside the closed enclosure, and opening into a buffer solution (28) contained in this container flask, at least one check valve (32) equipping each evacuation duct, and the chemical aging device comprising an agitation device (34) for agitating the test receptacles.

The application belongs to the field of functional materials and surface engineering, and particularly relates to an ant nest-like photo-thermal super-hydrophobic sponge as well as a preparation method and application thereof. The preparation method comprises the following steps: repeatedly soaking melamine sponge in anhydrous ethanol solution and drying; adding dopamine hydrochloride into a tris buffer solution to obtain a mixed solution by stirring, and then immersing the surface of the dried melamine sponge into the mixed solution and drying to obtain PDA@MS; adding PDMS and a curing agent into a cyclohexane solution to stir, then adding MWCNTs, MnO2 and CuO to stir to form a mixed solution, and then spraying the mixed solution on the surface of the PDA@MS and drying to obtain PMOS; and immersing the surface of the PMOS into a mixed solution of ethanol and deionized water containing PFOTES, and then taking out and drying. Through fine cross-scale multi-layer gap structure design, high mechanical durability, excellent light absorption capacity, efficient anti-icing, de-icing, interface evaporation and fresh water production in extreme environments are realized.

The invention provides a capillary electrophoresis analysis method of serum amino acid. The capillary electrophoresis analysis method comprises the following steps: S1, preparing an operation buffer solution; s2, selection and pretreatment of a quartz capillary column; s3, conditions are analyzed through electrophoresis; s4, drawing a quasi curve; s5, sample derivatization; s6, quantitative analysis. According to the method, a mixed reagent of alpha-cyclodextrin, beta-cyclodextrin and gamma-cyclodextrin is adopted for the first time, the amino acid migration behavior is cooperatively adjusted, a migration time window is expanded, the separation resolution of amino acids with similar structures is effectively improved, and the separation degree and the atlas definition are remarkably improved. According to the invention, sodium taurocholate is added, pH is regulated and controlled, a weakly alkaline buffer system with moderate conductivity and stable migration behavior is formed, separation and detection of FITC derived amino acids are facilitated, and balance of migration time distribution is optimized. The method is suitable for scenes of clinical sample analysis, disease marker screening and the like, and has good universality, stability and popularization value.

The invention discloses a preparation process of carboxylated shell-based activated carbon for organic matter adsorption, and particularly relates to the technical field of adsorption materials, and the preparation process comprises the following steps: mixing coconut shell powder and walnut shell powder, crushing, and loading potassium hydroxide in stages in a gradient manner; carrying out two-stage microwave activation in an inert atmosphere, namely low-temperature pre-activation in the first stage and high-temperature activation in the second stage, and introducing carbon dioxide; the activated product, phosphoric acid, a laccase-peroxidase complex enzyme preparation and a buffer solution are subjected to ultrasonic synergistic modification, and then a weak acid reaction is maintained through dynamic pH regulation and control; and finally, performing graded washing and gradient drying to obtain a finished product. According to the method, high-density carboxyl grafting and graded pore construction are synchronously realized under mild conditions through a synergistic effect of directional catalysis of biological enzyme and dynamic regulation of microwave, so that the problems of structural damage caused by strong acid corrosion, pore imbalance caused by fixed parameters and stability reduction caused by insufficient post-treatment in a traditional process are solved; the adsorption performance and the regeneration cycle performance of the material are also obviously improved.

The invention provides synthesis of a T-2 toxin antigen and preparation and application of a monoclonal antibody. Relates to the technical field of biotoxin detection. The preparation method of the T-2 toxin antigen comprises the following steps: step 1, synthesizing an immunogen T-2-BSA by adopting a carbodiimide method, dissolving 2mg of T-2 adipic acid with 0.5 mL of dimethylformamide, and recording as a solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved with a 2-morpholine ethanesulfonic acid buffer solution with the pH being 5.5, and the solution is marked as a solution B; step 2, under the condition of stirring, dropwise adding the solution A into the solution B, stirring for 18 hours in a dark place at 4 DEG C, then dialyzing for 3 days by using a phosphate buffer solution, changing the solution once a day, centrifuging the dialysate for 10 minutes at 3,500 r / min, and discarding the precipitate; the adipic acid is coupled with the T-2 toxin through a chemical method, and the unique step enriches the structural characteristics of the hapten, so that the prepared T-2 toxin complete antigen is excellent in immune effect, can efficiently excite immune response of an organism, and shows high specificity and sensitivity.

The invention provides a preparation method of biomass-derived porous carbon with ionic adsorption potential, which comprises the following steps: adding disodium ethylene diamine tetraacetate and ferrous chloride into water, and uniformly mixing to obtain EDTA-Fe; the preparation method comprises the following steps: sequentially adding an acetic acid-sodium acetate buffer solution and a biomass material into EDTA-Fe, uniformly mixing, adding aluminum chloride, carrying out a hydrothermal reaction to obtain a primary product, cleaning the primary product, and drying to obtain biomass hydrothermal carbon; the preparation method comprises the following steps: soaking biomass hydrothermal carbon in a saturated solution of potassium carbonate, then carrying out solid-liquid separation, firstly drying a solid obtained by separation, then calcining in a nitrogen atmosphere, and cleaning to obtain the biomass derived porous carbon. The biomass hydrothermal carbon prepared by the method has regular and controllable morphology, abundant surface functional groups and a stable carbon skeleton structure, and shows excellent electrochemical performance.

The invention relates to the technical field of rice seed germination accelerating systems and methods. Seed selection is conducted, specifically, rice seeds which are full and free of damage are screened, and impurities are removed; the rice seeds are soaked in a plant growth regulator mixed solution to be subjected to oscillation treatment, the mixed solution is composed of gibberellin with the concentration being 20-50 mg / L, pimacol with the concentration being 5-15 mg / L and polyoxyethylene lauryl ether with the concentration being 0.01-0.03%, the liquid temperature is kept at 30 + / -2 DEG C, and the treatment time is 2-4 hours; gradient disinfection: primary disinfection: putting the pretreated seeds into a 0.3-0.5% hydrogen peroxide solution at the temperature of 40-50 DEG C, and stirring for 15-20 minutes; secondary disinfection: transferring to a 0.8-1.2% sodium hypochlorite solution at the temperature of 25-30 DEG C, adding a 0.05-0.1 mol / L phosphate buffer solution to adjust the pH value to 6.8-7.2, and standing for 40-60 minutes; the disinfected seeds are put into magnetized water containing 0.1%-0.3% of chitosan derivatives and 0.05%-0.15% of sodium alginate, the magnetic field intensity ranges from 300 gauss to 500 gauss, and ultrasonic waves of 30 kHz to 50 kHz are adopted for assisting permeation for 1 h to 2 h; the rice seed germination accelerating system and method are high in germination accelerating efficiency.