134 results about "Buffer solution" patented technology

The present application relates to a controllable preparation method of a colloidal crystal capillary column and its application in biomolecule separation, and specifically comprises the following steps: a plunger and an electroosmotic pump are prepared in situ at one end of a capillary; a buffer solution containing monodisperse colloidal particles is filled in an upper reservoir of an electric field assembly device, and a buffer solution is filled in a lower reservoir; the capillary is filled with the buffer solution, and the end of the capillary containing the electroosmotic pump is inserted into the lower reservoir; the dispersion of the colloidal solution is maintained by applying oscillation to the upper reservoir, an electric field is applied between the two reservoirs to make the colloidal particles move to the electroosmotic pump under the action of an electroosmotic flow, and a three-dimensional colloidal crystal is assembled; and the capillary is supplemented with an ultrasonic force to reduce lattice defects. The present application has the advantages of simple preparation process, controlled directional movement of colloidal particles through an electric field, improved assembly efficiency and quality of the three-dimensional colloidal crystal, and controllable assembly of the colloidal particles under the action of an electric field through regulation and control of the length and surface charge of the electroosmotic pump.

The application discloses a method for synthesizing high-concentration furfuryl alcohol by using a double-enzyme coupling method, and the method comprises the following steps: mixing alcohol dehydrogenase freeze-dried bacterial powder and glucose dehydrogenase freeze-dried bacterial powder to serve as a catalyst, using furfural as a substrate, using glucose as a co-substrate, using NADP + or NAD + as a coenzyme, using a buffer solution with a pH value of 4-9 as a reaction medium to form a reaction system, and reacting completely under the condition of 20-50 DEG C and 400 rpm, then separating and purifying the reaction liquid to obtain furfuryl alcohol. The biological catalytic system is suitable for furfural with a concentration higher than 500 mM, and 100% conversion of 500 mM can be realized within 10 hours. The substrate continuous flow process is further used to eliminate the inhibition of aldehyde substrates on the biological catalyst, the concentration of the product can reach 1500 mM, and the highest space-time yield can reach 235 g / (L.d).

The application discloses a kind of composite super-hydrophobic paper based on alkyl ketene dimer and metal polyphenol network and preparation method thereof, belong to functional paper-based material field.The method is first immersed in 0.25%~5% alkyl ketene dimer emulsion in cellulose paper, is dried at 90~110 DEG C and is cured 48-96h to prepare modified paper;Then, the pH is 8.0-9.0 of tris-hydroxymethyl aminomethane hydrochloride buffer solution, hydrochloric acid dopamine is dissolved in the buffer solution to form dopamine solution, and the concentration is 0.25~20mg / mL of metal ion solution;Finally, the modified paper is immersed in dopamine solution and metal ion solution in turn, and is dried to obtain composite super-hydrophobic paper.The obtained composite super-hydrophobic paper uses cellulose paper-based material as substrate, and hydrophobic layer and metal polyphenol network layer are formed on the surface in turn, the composite paper has super-hydrophobic performance, water barrier performance and photo-thermal response performance, the preparation process is simple, and is suitable for waterproof packaging, self-cleaning surface, anti-icing and deicing and photo-thermal antibacterial and other fields.

This invention relates to the field of automatic control technology, specifically to a method for stabilizing and controlling the effective chlorine content of sodium hypochlorite solution. During the cooling stage of the sodium hypochlorite solution, an online electrochemical sensor acquires the concentration signal of free trace metal ions in the solution. When the signal reaches a preset threshold of the controller, the controller instructs a peristaltic pump to add a compound stabilizer to the solution. The compound stabilizer is prepared by mixing a specific short peptide containing cysteine ​​and histidine with a silicate buffer solution. After adding the compound stabilizer, the solution undergoes short-term ultrasonic cavitation treatment. This method uses a specific short peptide instead of traditional aminopolycarboxylate salts, avoiding the breaking and failure of carbon-nitrogen bonds under strong oxidizing conditions, preventing secondary release of metal ions that could trigger catalytic decomposition, extending the effective chlorine half-life, and reducing the amount of chlorate byproducts generated.

The application discloses an anticoagulant biomaterial for blood purification and a preparation method and application thereof, and comprises the following steps: providing an amphiphilic copolymer P(AA-MMA); blending polyethersulfone, P(AA-MMA) and a solvent, stirring at a set temperature to obtain a casting solution, and preparing a blending membrane from the casting solution; soaking the blending membrane in a 2-morpholine-4-yl ethanesulfonic acid buffer solution containing argatroban, 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide and hydroxy succinimide, and reacting at room temperature to obtain an argatroban grafted blending membrane, namely the anticoagulant biomaterial. The method improves the hydrophilicity of the membrane, reduces the albumin adsorption of the membrane, reduces the adhesion of platelets on the membrane, prolongs the coagulation time APTT, PT and TT of the membrane, and is better in biocompatibility and enhanced in anticoagulation performance.

The application discloses a capillary open-tube chromatographic column preparation method based on a defect engineering metal organic framework-molecular imprinting composite nanomaterial and application, and particularly relates to the field of chemical synthesis and analysis technology. A GLYMO-IDA-silane solution is used to flush a pretreated capillary, so that a carboxyl-modified capillary is obtained. Then, an N,N-dimethylformamide solution containing zirconyl chloride octahydrate, terephthalic acid, L-proline and hydrochloric acid is injected into the cysteine-modified capillary, and under a heating condition, a metal organic framework UiO-66-L-Pro is in-situ grown on the inner wall of the capillary. D-tryptophan and norepinephrine are dissolved in a neutral buffer solution, and after magnetic stirring for 30 min, ferrous sulfate is added and rapidly injected into the UiO-66-L-Pro-modified capillary, and then both ends are sealed, and reaction is carried out at room temperature for 24 h. An acetic acid / SDS solution mixture is used to flush the capillary for 1.5 h to elute the template molecules, and then nitrogen is used to blow dry, so that an MIP@UiO-66-L-Pro@capillary is prepared.

This invention relates to the field of down fabric technology, specifically to a mite-proof and antibacterial composite down and its preparation method. The mite-proof and antibacterial composite down includes two outer fabric layers, two inner down-proof fabric layers, two nanocellulose / chitosan composite layers, and down filling. The nanocellulose / chitosan composite layers are prepared from the following components: aqueous polyurethane emulsion, nanocellulose, chitosan, chitosan-modified wood vinegar powder, natural genipin, and a dispersant. Chitosan is added in the form of an acetate-sodium acetate buffer solution with a pH of 4.0–4.8. This invention, through the synergistic effect of chitosan-modified wood vinegar powder and nanocellulose, combined with an aqueous polyurethane matrix, can simultaneously achieve high repellency, high antibacterial rate, and excellent wash resistance. Furthermore, the nanocellulose enhances the interfacial bonding strength of the nanocellulose / chitosan composite layer, significantly improving interlayer adhesion strength and down-proof performance.

This invention relates to the field of high-purity material analysis technology, and discloses a method for simultaneously separating and enriching copper, cadmium, nickel, cobalt, zinc, lead, and barium in lithium carbonate. The steps are as follows: dissolving a lithium carbonate sample in nitric acid to obtain an acidified sample solution; adjusting the acidified sample solution to an alkaline pH range of 9.5-10.5 using an ammonia-ammonium acetate alkaline buffer solution; passing the alkaline solution through a solid-phase extraction column filled with iminodiacetic acid-type chelating resin, where copper, cadmium, nickel, cobalt, zinc, lead, and barium ions are selectively adsorbed onto the resin; and eluting the adsorbed heavy metal ions with acid to achieve separation and enrichment. This invention effectively overcomes the inhibition and interference of the lithium carbonate matrix on the detection of trace heavy metals, and in particular significantly improves the adsorption and recovery rate of elements such as barium. It achieves efficient separation and enrichment of multiple heavy metals in complex matrices, providing a reliable pretreatment solution for high-sensitivity and high-accuracy quality control analysis of lithium carbonate products.

A method for improving the accuracy of pH value detection using an antimony-based pH electrode, belonging to the field of pH value detection technology, includes: measuring the dissolved oxygen concentration of the test solution; comparing this dissolved oxygen concentration with the dissolved oxygen concentration of the standard buffer solution used during pH sensor calibration; when the two dissolved oxygen concentrations differ, dissolved oxygen potential compensation is required: multiplying the difference between the two dissolved oxygen concentrations by a dissolved oxygen concentration compensation coefficient to obtain a dissolved oxygen potential compensation value; and using this dissolved oxygen potential compensation value to compensate the electrode potential of the antimony-based pH electrode in the test solution, thereby achieving accurate pH value measurement. This invention improves the measurement accuracy of the antimony-based pH electrode and the accuracy of in-situ pH value monitoring results through dissolved oxygen potential compensation.

The application relates to the technical field of nanomaterials, in particular to a hydrogen-bonded organic framework loaded nanoseelenium material and a preparation method and application thereof, wherein the preparation method comprises the following steps: preparing a hydrogen-bonded organic framework material HOF; resuspending the prepared HOF in a bovine serum albumin aqueous solution, stirring and reacting, centrifugal washing and concentrating, and resuspending in a buffer solution; on the basis of the above solution, adding a sodium selenite solution dissolved in the buffer solution to continue stirring, adding a reducing agent to react, and growing nanoseelenium SeNPs on the surface of the HOF in situ; centrifugal washing and purifying the obtained product, resuspending in a polyethylene glycol aqueous solution to react, centrifugal washing and concentrating, and obtaining HOF@SeNPs. The prepared HOF@SeNPs are suitable for the fields of plant selenium-rich cultivation, crop heavy metal pollution control, animal cadmium poisoning relief, selenium-rich animal product feeding and the like, and provide a new idea for green agriculture and environment remediation and selenium element strengthening of agricultural products.

The application discloses a lysozyme content detection method in food, and belongs to the technical field of biological medicine detection. The method comprises the following steps: constructing a lysozyme detection standard curve; reacting a food sample to be detected with a fluorescent probe in a buffer system, measuring the fluorescence intensity of the reaction system, and calculating the lysozyme content in the food sample to be detected based on the standard curve. The application adopts a fluorescence spectrum method to research and compare the performance of polypeptide CALNNK (FITC) and lysozyme under different buffer systems, pH, temperature and reaction time conditions, and then uses the method to detect the lysozyme content in food. The 20 nmol / L polypeptide CALNNK (FITC) , BR buffer solution with pH=10.0, 4 min of time and 35 DEG C of temperature are the optimal reaction conditions, the lysozyme content in food can be quickly and accurately detected, and the method has far-reaching significance for the healthy development of the food industry.

This invention discloses a selenium-enriched dairy product from selenium-enriched dairy cows and its preparation method, comprising the following components and process characteristics: Basic milk matrix: by mass percentage, containing 85-95% raw milk or reconstituted milk, 0.5-2% milk protein concentrate, and 0.1-0.5% fructooligosaccharides; Probiotic-polyphenol complex: composed of *Lactobacillus plantarum* LP-33 (preservation number CGMCC No. 24567) and grape seed polyphenols, wherein the polyphenols are compounded in a 1:1 ratio with zein-chitosan bilayer microencapsulated form in a free state, with a total addition amount of 50-100 mg / L; Dynamic pH control system: during fermentation, the pH value is monitored in real time and a 0.1-0.5 mol / L sodium bicarbonate buffer solution is automatically added to maintain the pH at 5.0-5.5. This invention achieves breakthroughs in viable count and acid resistance; through dynamic pH control and synergistic protection by polyphenols, the viable count of the finished product is ≥1×10⁻⁶. 9 With a CFU / mL concentration, the survival rate of probiotics in simulated gastric juice for 2 hours is >80%, solving the problem of "high output but low intake" of probiotics in traditional fermentation.

This invention discloses a novel cobalt ion highly sensitive and selective colorimetric reagent and its applications. The structural formula of the novel cobalt ion colorimetric reagent is shown below. The azo colorimetric reagent of this invention showed high sensitivity to Co in the detection of seventeen metals. 2+ For specific detection, a buffer solution and surfactant are added to the test solution, followed by the addition of a colorimetric reagent. The Co content in the solution... 2+ It forms a stable orange-red complex with the colorimetric reagent in an acidic environment. The maximum absorption wavelength of the cobalt complex is 426 nm, and the detection limit of cobalt is 5 × 10⁻⁶. ‑3 mg / L, detection limit is 1×10 ‑2 The detection limit is low and the sensitivity is high (mg / L). This invention uses spectrophotometry for cobalt ion detection, where the chromogenic compound is an environmentally friendly material that does not cause environmental pollution during the detection process. It does not require large instruments and equipment, and is simple, efficient, and convenient to operate.

This invention provides a highly adhesive, wide-temperature-range aqueous hydrogel electrolyte for zinc-ion batteries, its preparation method, and its applications. The method includes the following steps: dissolving sodium alginate in 2-morpholine ethanesulfonic acid buffer solution, adding EDC and NHS, and activating; then adding dopamine hydrochloride, undergoing an amidation reaction, dialysis purification, and freeze-drying to obtain SA-DA powder; adding gelatin and SA-DA powder to deionized water, stirring and mixing evenly, and allowing to stand; then immersing in the electrolyte and undergoing soaking treatment to obtain the final product. The electrolyte of this invention exhibits excellent interfacial adhesion and flexible mechanical properties, significantly improved electrochemical stability and cycle life, excellent wide-temperature-range adaptability and anti-drying ability, outstanding safety and biocompatibility, and a simple and highly controllable process, providing key technical support for the development of high-performance, high-safety flexible aqueous zinc-ion batteries.

The present application relates to the technical field of polyester degradable plastics, and particularly relates to a bio-degradable bubble film bag puncture resistance reinforcement process. The bio-degradable bubble film bag puncture resistance reinforcement process comprises the following steps: dust and oil removal on the surface of a formed bubble film bag, heat treatment to obtain a pretreated bubble film bag; soaking the pretreated bubble film bag in a Tris-HCl buffer solution, adding dopamine hydrochloride into the Tris-HCl buffer solution, air bubbling and ultrasonic treatment, vacuum drying to obtain a surface-activated bubble film bag; adding a silane coupling agent into an ethanol aqueous solution, stirring, adding nano calcium carbonate and graphene oxide into the ethanol aqueous solution, stirring, filtering, washing, vacuum drying to obtain an activated filler; adding polylactic acid and polylactic acid grafted maleic anhydride into dichloromethane, stirring until uniform, adding the activated filler into the dichloromethane, stirring, adding a leveling agent, continuing to stir, standing and defoaming, low-temperature spraying onto the surface of the surface-activated bubble film bag, roller compounding, heat preservation and natural cooling.

This invention discloses a GSH-functionalized carbon dots / rhodamine B ratiometric fluorescence system, its construction method, and its application in nitrite detection. GSH-CDs are synthesized via a one-step hydrothermal method by mixing citric acid, o-phenylenediamine, glutathione, and hydrochloric acid in water. Unreacted precursors and small molecules are removed by purification, and the GSH-CDs are obtained by freeze-drying. The ratiometric fluorescence system is constructed by mixing rhodamine B, GSH-CDs, and an acidic buffer solution. The fluorescence intensity ratio F of GSH-CDs to rhodamine B is established. 460 / F 590 With nitrite (NO2) ‑ A calibration curve between concentrations was obtained, and the NO2 in the actual sample was experimentally detected using the spiked recovery method. ‑ Content. Under acidic conditions, with NO2... ‑ With increasing concentration, the GSH-CDs / RhB fluorescence intensity ratio (I 460 / I 590 Significant changes occurred, with the fluorescence intensity ratio compared to NO2. ‑ There is a good linear relationship between concentrations. Compared with the detection platform based on GSH-CDs, the ratio fluorescence system provided by this invention has a lower detection limit and significantly higher sensitivity.

This invention relates to the field of electrochemical detection technology for vitamin E, specifically to an electrochemical detection device and method for vitamin E. The method includes: utilizing a detection system composed of a glassy carbon electrode, a saturated calomel electrode, and a platinum wire electrode; performing a series of optimizations and screenings to determine the SWV electrochemical detection method; and electrode pretreatment: using 0.05 μm alumina powder to perform circular polishing on the glassy carbon electrode in both directions for 3-4 minutes, followed by ultrasonic cleaning with anhydrous ethanol and pure water for three minutes each time. Finally, the electrode is scanned using the SWV method in a prepared buffer solution until it stabilizes. The beneficial effects are: this invention is convenient and simple to operate, has a simple formulation that greatly optimizes the detectable limit, has low manufacturing cost and is readily available, and requires no electrode modification; the lowest detectable concentration is far below the minimum normal range of vitamin E in human blood.

This invention provides a method for rapid extraction of collagen, belonging to the field of regenerative medicine technology. The method includes the following steps: (1) taking animal tissue material as the extraction raw material, crushing it to remove fat and impurities, and obtaining a precipitate; (2) placing the precipitate into a molding mold to obtain a tissue module, freezing and storing the tissue module to obtain a pretreated material; (3) mixing an acidic aqueous solution and pepsin to obtain an extract; placing the pretreated material in the extract in a suspended state, first allowing it to stand and then extracting it in suspension, then stirring, filtering or centrifuging, and retaining the supernatant to obtain a crude extract; (4) adding inorganic salts to the crude extract, allowing it to stand, filtering, collecting the precipitate, washing it, and then transferring the precipitate into a buffer solution to swell it. Filtering the swollen precipitate and dialyzing it to obtain a collagen aqueous solution, followed by post-processing to obtain collagen. The method for extracting collagen provided by this invention can significantly shorten the extraction time.

To ensure the temperature performance of reagents on a slide without causing the sliding of a heat transfer surface caused by vibration, the specimen slide stirring device of the present invention stirs and mixes a buffer solution on a specimen slide on which a specimen is held and a staining reaction reagent required for staining the above-mentioned specimen, by vibrating, is provided with a slide stage that holds the above-mentioned specimen slide, a temperature adjustment section that performs temperature adjustment of the above-mentioned specimen slide, a heat transfer section that transfers heat to the above-mentioned specimen slide in a manner that makes the temperature of the above-mentioned temperature adjustment section and the above-mentioned specimen slide substantially constant, and a lift stage that lifts and lowers the slide holding section, when stirring the above-mentioned buffer solution and the above-mentioned staining reaction reagent, the above-mentioned lift stage is lowered to separate the above-mentioned heat transfer section from a vibration section that vibrates the above-mentioned buffer solution and the above-mentioned staining reaction reagent before the above-mentioned buffer solution and the above-mentioned staining reaction reagent are vibrated, and the above-mentioned lift stage is raised to bring the above-mentioned heat transfer section into contact with the above-mentioned vibration section when heat transfer is performed to the above-mentioned specimen slide via the above-mentioned heat transfer section.

The present application relates to the technical field of cosmetic biotechnology and nano-carrier drug delivery, and provides PDRN-putrescine co-delivery nano-liposome as well as a preparation method and application thereof.The PDRN-putrescine co-delivery nano-liposome provided by the present application comprises the following components in mass fraction: 0.1-3% of polydeoxyribonucleotide; 0.1-3% of putrescine; 2-8% of phospholipid emulsifier; 0-15% of polyhydric alcohol cosolvent; and the balance is a buffer solution; and the buffer solution has a pH value of 6-7.5.The present application realizes stable co-encapsulation of PDRN and putrescine by constructing a specific buffer system to regulate the pH value, using the amphiphilic property of the phospholipid bilayer and the in-situ ion pair technology, solves the compatibility problem of PDRN and putrescine, significantly improves the transdermal absorption rate of PDRN through transmembrane delivery of the nano-carrier, and realizes the synergistic repair and anti-aging effect of PDRN and putrescine at the cellular level.

The application relates to a method for fluorescent detection of iron ions in a water sample by using luminescent sulfur-phosphorus quantum dots, and the method specifically comprises the following steps: dissolving appropriate luminescent sulfur-phosphorus quantum dots in a buffer solution to obtain a detection mother liquor, the detection mother liquor can be prepared into different concentrations according to needs, and the detection mother liquor is added into a sample to be detected, and the detection mother liquor is used for detecting the iron ions in the water sample through a fluorescence quenching method. The application utilizes the fluorescence characteristics of the sulfur-phosphorus quantum dots to quickly (less than 60s) detect the iron ions, and the material has good selectivity for the iron ions in different water samples.

This invention discloses a copper / hydrogen peroxide synergistic-induced dopamine surface self-polymerization coating and its preparation method, relating to the field of surface and interface modification technology. The specific preparation method is as follows: Buffer solution A is prepared using an organic amine buffer solution; copper salt is added and ultrasonically dispersed to obtain a copper-containing solution B; a substrate pretreated with acid washing is immersed in solution B; a dopamine precursor is added to obtain a mixed solution C; then an aqueous hydrogen peroxide solution is added, and the reaction is carried out under light-protected conditions to form a dopamine polymer coating in situ on the substrate surface. After washing and drying, a modified substrate with a copper / hydrogen peroxide synergistic-induced dopamine coating is obtained. 2+ It exhibits a significant synergistic effect with H2O2 in inducing oxidative polymerization, reflecting the combined effect of coordination site saturation and subsequent layer coverage. This invention achieves higher film-forming efficiency and interfacial stability through short-time impregnation and optimized reaction sequence, making it suitable for rapid modification of glass-like inert substrates.

The application discloses a lipoic acid high-performance liquid chromatography detection method, which comprises the following steps: S1, sample pretreatment: ultrasonic extraction is carried out on the sample by using a composite extraction liquid, then solid-liquid separation is carried out, and a test sample solution is obtained; the composite extraction liquid is an acetonitrile water mixed solution containing cysteine and EDTA salt; S2, HPLC detection: the chromatographic conditions are as follows: a C18 reversed-phase chromatographic column is used, a buffer solution-alcohol mixed system is used as a mobile phase, the pH of the buffer solution is 2.5-3.5, the detection wavelength is 210-220 nm in the first stage and is 275-285 nm in the second stage, the first stage is 0-7-9 min, and the second stage is 7-9 min-14-16 min. By optimizing the pretreatment method, optimizing the chromatographic conditions and adopting a dynamic detection wavelength, the lipoic acid content detection method with high sensitivity and strong anti-interference ability is obtained.

This invention discloses a method for detecting adiponectin in human serum using a polydopamine-modified wire mesh carbon electrode, specifically relating to the field of adiponectin detection technology. The method involves preparing a 2 mg / mL dopamine hydrochloride solution using a 10 mM tris(hydroxymethyl)aminomethane hydrochloride buffer solution (pH=8.5) and adding it dropwise onto the surface of the working electrode. A polydopamine-modified layer is formed by electropolymerization within a range of -800 mV to 600 mV using cyclic voltammetry. A conductive layer of a poly(3,4-ethylenedioxythiophene)-polystyrene sulfonic acid complex is then coated. Adiponectin antibodies are then immobilized on the surface of the modified layer via covalent binding of amino and quinone groups. After specific binding with a serum sample, the oxidation peak current change is read out from -100 mV to 350 mV in a buffer detection medium containing 5 mM ferricyanide redox pairs, and the concentration is output from a standard curve. This method improves the density of antibody immobilization sites and inhibits non-specific serum adsorption, enhancing signal stability and sensitivity, and enabling rapid, low-cost quantitative detection of trace amounts of serum.

This invention discloses a purification method for Tirzepatide. The method includes: dissolving crude Tirzepatide peptide in ammonia or sodium bicarbonate solution, filtering, and purifying by reversed-phase chromatography to obtain a purified sample; eluting the purified sample using reversed-phase high-performance liquid chromatography (RP-HPLC) with a sodium or potassium buffer solution as mobile phase A3 and one or a mixture of acetonitrile, methanol, and isopropanol in any proportion as mobile phase B, preparing a sodium or potassium salt fraction; cooling the sodium or potassium salt fraction to -20 to 10°C and maintaining the temperature; then adding an organic solvent to the fraction, controlling the temperature at -20 to 10°C to precipitate the sample; after complete precipitation, solid-liquid separation is performed, the solid is collected, dissolved in water, filtered, and freeze-dried. This invention improves the concentration speed, ensures product purity, and strictly controls impurities.

This application provides a method for detecting sodium dodecylbenzenesulfonate (SDBS) in plant samples, comprising: washing, freeze-drying, pulverizing and sieving the plant sample to obtain plant sample powder; performing multiple ultrasonic-assisted extractions on the plant sample powder using a methanol-water mixed solvent; combining and collecting the extracts; diluting the combined extracts with water; loading the extracts onto a weak anion exchange solid-phase extraction column for purification; eluting with ammonium acetate buffer solution; eluting with an organic solvent containing alkaline components; collecting the eluent containing the target analyte; concentrating the eluent to near dryness under controlled temperature conditions with nitrogen blowing; redissolving the eluent; filtering the eluent through a filter membrane to obtain the test solution; and qualitatively and quantitatively analyzing the sodium dodecylbenzenesulfonate in the test solution using liquid chromatography-tandem mass spectrometry (LC-MS / MS). This invention overcomes the interference of complex components in the plant matrix through a specific extraction system and selective purification, achieving quantitative analysis of SDBS.

This invention relates to the field of radioactive separation technology, and discloses an automated solution preparation and delivery control method and system for At-211 separation, comprising: Step 1, determining the column bed volume, setting the minimum operating volume particle size, and determining the pump running time; Step 2, solidifying the reagent and matching it with the valve inlet, obtaining the background current and determining the low threshold current, high threshold current, tail threshold current, and volume confirmation rules for the buffer solution; Step 3, generating the loading solution; Step 4, delivering the low-acid washing column solution, buffer solution, and preset equilibration solution to obtain the separation column; Step 5, loading the sample and comparing the output current of the ionization chamber with the low threshold current and high threshold current to determine the three states; Step 6, switching the collection valve to the product bottle and cutting off the organic elution solvent; Step 7, controlling the collection valve and determining the actual product volume. This invention achieves automated and quantitative control of solution preparation, delivery, elution, and collection during the At-211 separation process.

The present application relates to the technical field of in vitro diagnosis, disclose a kind of laminin determination kit and preparation method thereof, the reagent kit is composed of reagent 1 and reagent 2, the main component of reagent 1 has ammonium chloride, sodium azide, Brij-35, sodium chloride, PEG6000, EDTA2Na, F (ab') 2 fragment, the main component of reagent 2 has mouse monoclonal antibody coated latex microparticle, Tris-Hcl buffer solution, sucrose, gelatin, Tween-20, NP30, Proclin300, mouse monoclonal antibody coated latex microparticle in the reagent kit has good suspensibility and monodispersity, stably exists in aqueous system, improves the determination intensity and stability of signal. The kit is specific, and high accuracy.

This invention discloses a nickel-based dithioene-transition metal MOFs / MWCNTs / GCE 5-HT electrochemical sensor, comprising a working electrode, a reference electrode, a counter electrode, and a buffer solution. NiZn-MOFs are prepared using P2S5, dioxane, dimethyl 4,4'-(2-hydroxyacetyl)dibenzoate, NiCl2·6H2O, THF, KOH, and Zn(NO3)2·4H2O. These are then ultrasonically composited with carboxylated carbon nanotubes to obtain NiZn-MOF / MWCNTs. A glassy carbon electrode is modified to obtain the working electrode. This electrochemical sensor can be used for 5-HT detection. The multi-walled carbon nanotubes exhibit good conductivity, and their combination with the redox-active three-dimensional NiZn-MOFs with unsaturated coordination sites improves the conductivity and catalytic performance of the composite material, solving the problems of low electrocatalytic activity and low sensitivity in 5-HT detection.

The application discloses a multifunctional composite binder for a silicon-carbon negative electrode of a lithium ion battery and a preparation method and application thereof, and belongs to the technical field of lithium batteries. The method comprises the following steps: dissolving sodium alginate in a PBS buffer solution, adding a catalyst and 1-pyrenemethylamine hydrochloride, and performing an amidation reaction under room temperature conditions; after the reaction is completed, precipitation, suction filtration and freeze-drying are performed to obtain SA-Py; water is added into the SA-Py to obtain an SA-Py aqueous solution, and then carboxylated carbon nanotubes are dispersed in the SA-Py aqueous solution, so that the multifunctional composite binder is obtained. The binder prepared by utilizing multiple synergistic effects can effectively inhibit the volume expansion of a silicon-carbon negative electrode material in a cycle process, so that the long-term cycle stability of the electrode is significantly improved.