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Polysaccharide-dopamine composite biogel and application thereof

A technology of dopamine and biological glue, applied in the field of biological glue, can solve problems such as poor adhesion ability, and achieve the effects of reducing loss, increasing tissue viscosity, and increasing surface viscosity

Active Publication Date: 2014-09-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a polysaccharide-dopamine composite bioglue and its application in repairing superficial cartilage defects, specifically to successfully modify dopamine on polysaccharide molecules through chemical synthesis to prepare a new type of polysaccharide-dopamine composite bioglue , using the oxidative self-polymerization properties of dopamine to form a layer of polysaccharide-dopamine coating on the surface of cartilage to improve the poor adhesion of cells and bioactive molecules on the surface of cartilage, while maintaining the original structure of the cartilage surface, which can effectively improve superficial cartilage. defect repair ability

Method used

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  • Polysaccharide-dopamine composite biogel and application thereof
  • Polysaccharide-dopamine composite biogel and application thereof
  • Polysaccharide-dopamine composite biogel and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1: Chondroitin sulfate-dopamine composite biological glue and its application

[0033] (1) Preparation of chondroitin sulfate-dopamine composite bioglue: In a nitrogen environment, add 200 mg of chondroitin sulfate sodium salt (that is, chondroitin sulfate, purchased from sigma) into 40 mL of MES buffer (0.1M, pH5.5), Stir until completely dissolved to make a polysaccharide solution. In a nitrogen environment, dissolve 218mg EDC and 247mg sulfo-NHS in 1mL MES buffer (0.1M, pH5.5), and make an EDC mixture after completely dissolved; then add the EDC mixture to the polysaccharide in a nitrogen environment In the solution, the reaction was stirred at 25°C for 30 min. 216.2 mg of dopamine was dissolved in 1 mL of MES buffer (0.1M, pH 5.5), added to the above reaction solution, and reacted for 120 minutes at 25°C under nitrogen. The product after the reaction was taken out, placed in a dialysis bag (MWCO3500), and dialyzed in distilled water for 3 days; finally, the p...

Embodiment 2

[0042] Example 2: Chondroitin sulfate-dopamine bioglue

[0043] (1) Preparation of chondroitin sulfate-dopamine bioglue: 200 mg of chondroitin sulfate was added to 40 mL of MES buffer (0.1M, pH 5.9), and stirred in a nitrogen environment until completely dissolved to prepare a polysaccharide solution. Dissolve 218 mg EDC and 247 mg sulfo-NHS in 1 mL MES buffer (0.1M, pH 5.9). After complete dissolution, an EDC mixture is obtained, which is added to the polysaccharide solution and reacted for 30 minutes at room temperature under nitrogen. 216.2 mg of dopamine was dissolved in 1 mL of MES buffer, added to the above reaction solution, and reacted for 60 minutes at room temperature (25° C.) under nitrogen. The product after the reaction was taken out, placed in a dialysis bag (MWCO3500), and dialyzed in distilled water for 3 days; finally, the product was freeze-dried (48 hours, 10Pa, -108°C) to obtain chondroitin sulfate-dopamine biojelly 192mg, And store at 4℃ in the dark.

[0044]...

Embodiment 3

[0046] Example 3: Sodium Hyaluronate-Dopamine Biogel

[0047] (1) Preparation of sodium hyaluronate-dopamine bioglue: 400 mg of sodium hyaluronate was added to 80 mL of MES buffer (0.1M, pH 5.9), and stirred in a nitrogen environment until completely dissolved to prepare a polysaccharide solution. Dissolve 437 mg EDC and 495 mg sulfo-NHS in 2 mL MES buffer (0.1M, pH 5.9). After complete dissolution, an EDC mixture is obtained, added to the polysaccharide solution, and reacted for 30 minutes at room temperature (25° C.) under nitrogen. Dissolve 432 mg of dopamine in 2 mL of MES buffer (0.1M, pH 5.9), add to the above reaction solution, and react for 60 minutes at room temperature (25° C.) under nitrogen. The product after the reaction was taken out, placed in a dialysis bag (MWCO8000~14000), and dialyzed in distilled water for 3 days; finally the product was freeze-dried (72 hours, 10Pa, -108°C) to obtain sodium hyaluronate-dopamine biogel 411mg, stored at 4°C and protected from ...

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Abstract

The invention discloses a polysaccharide-dopamine composite biogel and application of the biogel. A preparation method of the biogel comprises the following steps: dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide sodium in an MES (Methyl Ester Sulfonate) buffer solution with a pH value being 5-6 in an inert gas atmosphere so as to prepare an EDC (ethylcarbodiimide) mixed solution, subsequently adding the EDC mixed solution into a polysaccharide solution for reacting at 20-30 DEG C for 0.5-1 hour, then adding a dopamine solution for further reacting at 20-30 DEG C for 1-4 hours, dialyzing a reactant by taking distilled water as a dialyzate, taking trapped fluid for freezing and drying to obtain the polysaccharide-dopamine composite biogel. The polysaccharide-dopamine composite biogel is simple and effective in modification method and capable of increasing the surface viscidity of the cartilage tissue, reducing loss of therapeutic drugs or implanted cells and facilitating the repairing of the cartilage injury.

Description

(1) Technical field [0001] The invention relates to a biological glue, in particular to the preparation of a biological glue that can directly adhere to the surface of damaged cartilage and promote the adhesion of other biologically active substances or cells, and a method for coating the biological glue on the surface of the cartilage. (2) Background technology [0002] Cartilage is composed of a special extracellular matrix produced by chondrocytes, without blood supply, and its main components are hyaluronic acid, chondroitin sulfate, type II collagen, etc. Cartilage cells are usually located at the junction of osteochondral cells, and their number is limited and their migration ability is weak due to the limitation of extracellular matrix. Due to these characteristics of cartilage, it is difficult to heal after injury. In recent years, the "autologous chondrocyte transplantation" (ACI) technology has been developed in which healthy cartilage cells from the patient are extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08
Inventor 欧阳宏伟汪燕艳牟秦孙亨
Owner ZHEJIANG UNIV
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