136 results about "Biological glue" patented technology

The invention discloses a bullfrog extracted biological collagen composition and a preparation method thereof in the technical field of collagen. The bullfrog extracted biological collagen composition comprises the following components in parts by weight: 1-1.5 parts of hydrophobic bullfrog source collagen peptide, 0.6-0.8 part of hydrophobic bullfrog source elastin peptide, 1-1.5 parts of polyglutamic acid and 1-1.5 parts of polylysine. Collagen and elastin in bullfrog skin and bullfrog cartilage are extracted in a combined mode through a three-enzyme co-production method, and after bullfrog source collagen peptide and bullfrog source elastin peptide are subjected to hydrophobic modification, polyglutamic acid and polylysine are added to form a collagen combination. The stability, the medicine carrying capacity and the slow release capacity of the composition can be improved.

The invention provides a self-leveling underwater filling material. The self-leveling underwater filling material is prepared from the following raw materials in parts by weight: 15-18 parts of a low-carbon cementing material, 8-10 parts of fly ash, 45-50 parts of engineering slurry, 0.2-0.4 part of biological glue, 3-5 parts of plant residue charcoal, 45-50 parts of recycled fine aggregate, 10-20 parts of recycled micro powder and 10-15 parts of water. The invention also provides a preparation method of the self-leveling underwater filling material. The self-leveling underwater filling material provided by the invention has relatively good strength and working performance, and is relatively low in cost.

The invention relates to a preparation method of a biological capsule similar to aerobic granular sludge and a method for treating domestic sewage by using the biological capsule. The preparation method of the biological capsule comprises the following steps: (a) preparing a gel solution; (b) mixing the gel solution with microorganisms to form a mixture; (c) dropwise adding the mixture into a cross-linking agent solution, so that a gel network is formed and microorganisms are fixed in the gel network to form a biological capsule; and (d) placing the biocapsule in a cross-linking agent solution for 12-24 hours to further enhance the gel strength, and then rinsing with water. The sewage treatment method comprises the following steps: (i) adding a biological capsule into a reactor; and (ii) operating the reactor in a sequencing batch mode. Although the biological capsule is broken at the initial stage of operation of the reactor, the fragments of the biological capsule can realize stability recovery within two weeks. Similar to aerobic granular sludge, the biological capsule fragments have a compact structure, good settling performance and capability of synchronously removing organic matters and nutrient substances. The technical problems that the aerobic granular sludge culture process is long and culture conditions are severe are solved, and the application prospect is good.

The invention relates to the technical field of high polymer materials, in particular to biological glue applied to fixation of an esophageal hiatus hernia patch. The biological glue comprises 40 to 60 wt% of fibrinogen, 30 to 50 wt% of a polyethylene glycol polymer, 2 to 8 wt% of an amino-containing small molecule compound, 0.2 to 0.8 wt% of a growth factor and 1 to 3 wt% of glutaraldehyde. The biological glue provided by the embodiment of the invention has excellent mechanical properties and good histocompatibility, and can realize a better patch fixing effect in a hiatus hernia operation, so that postoperative recovery of a patient is guaranteed.

The invention provides an amnion-loaded contact lens and a preparation method and application thereof. According to the invention, the bonding mode of the amniotic membrane and the contact lens is innovated, the previous mode of simply bonding the amniotic membrane and the contact lens by using medical biological glue is changed, the bonding procedure of the amniotic membrane and the contact lens is simplified, and the method is suitable for industrial application; the problems that the mechanical supporting force is reduced, the amniotic membrane is broken and the coverage is uneven due to the fact that the ocular surface coverage time of a traditional suture type amniotic membrane is prolonged are solved; in the aspect of material optimization and selection, the basic requirement of non-toxicity and harmlessness of the adhesive is met, and meanwhile, the requirements of biocompatibility and the oxygen permeability of the cornea on the contact lens are also considered; the amniotic membrane is more comfortable in the using process, complex operation procedures are not needed, and stimulation caused by no suture is avoided; the design scheme that the contact lens is used as the amniotic membrane support enables the amniotic membrane lens to provide better support and protection for the covered ocular surface.

The invention relates to a biological glue patch applied to non-invasive repair of dura mater, and relates to the technical field of medical biological materials. The biological glue patch comprises a patch base material, a biological membrane layer is arranged on one side of the patch base material, a hydrophobic membrane layer is arranged on the outer side of the biological membrane layer, a vacuum cavity is formed between the inner side of the biological membrane layer and the side portion of the patch base material, and biological glue is arranged in the vacuum cavity; one side, facing the biological membrane layer, of the patch base material is provided with a stabbing part for stabbing the biological membrane layer and the hydrophobic membrane layer. According to the biological glue patch, repairing of the dura mater is achieved in a glue adhesion mode, pinholes are avoided, the phenomenon of leakage of cerebrospinal fluid is avoided, meanwhile, the biological glue is protected through the biological film layer and the hydrophobic film layer, and the biological glue is prevented from being solidified and adhered in advance; the biological glue is released by arranging the stabbing part, the operation is simple, and the device can be applied to repairing the dural mater injury by a non-invasive single-side dual-channel spine endoscope technology.

The invention provides a polyethylene glycol derivative-collagen cartilage repair gel which is characterized by being prepared from the following components in parts by weight: 8 to 12 parts of collagen (namely COL) solution, 0.5 to 2 parts of polydeoxyribonucleotide (namely PDRN), 4 to 8 parts of polyethylene glycol disuccinimide succinate (namely SS-PEG-SS) solution and 0.1 to 0.4 part of tannic acid (namely TA), and particularly discloses a preparation method and application of the polyethylene glycol derivative-collagen cartilage repair gel. In a word, the composite gel and the collagen are subjected to efficient amidation reaction through SS-PEG-SS to form a stable three-dimensional network, so that the structural support of the type I collagen, the tissue repair of PDRN and the anti-inflammatory and anti-oxidation characteristics of tannic acid are successfully integrated; therefore, the composite material has the comprehensive performance of good structural stability, high mechanical strength, good biocompatibility, controllable degradation period, excellent needle passing performance and the like, and shows the wide application prospects of clear repair effect, remarkable component synergistic effect, mature production process, outstanding cost effectiveness and the like.

The invention belongs to the field of biomedical materials, and particularly relates to a self-adhesion composite patch for tooth extraction cavity wound repair and a preparation method thereof. The self-adhesion composite patch comprises a wound filling layer and an adhesion blocking layer, the wound filling layer is located in the middle of the adhesion blocking layer, the wound filling layer is made of a biological sponge material, and the adhesion blocking layer is made of polymer fibers and biological glue. The self-adhesion composite patch has excellent hemostasis, repairing, blocking, biosafety, biodegradability and water absorption, due to the excellent water absorption and biodegradability, the wound filling layer can achieve the excellent hemostasis function under the condition of a small filling amount, and meanwhile, the self-adhesion composite patch has the advantages that the self-adhesion composite patch is good in hemostasis effect and good in barrier effect. Due to the reduction of the wound filling layer and the excellent degradation performance, the self-adhesion composite patch cannot generate adverse effects on wound healing, and secondary operation for taking out the patch is not needed.

A nested valved pipeline comprises an outer pipeline, a pipeline support on the inner wall of the outer pipeline and valve leaflets arranged on the pipeline support, 3-5 main ribs extend from the center of the root of each valve leaflet to the edge of each valve leaflet, secondary branch ribs are distributed among the main ribs, and the main ribs and the secondary branch ribs form reinforcing ribs for net-shaped supporting; the reinforcing ribs are embedded into the valve leaflets and are integrally formed with the valve leaflets; the root of the reinforcing rib extends to the attachment area of the valve leaflet and the pipeline support to form a T-shaped anchoring structure, an annular groove is designed in the edge, close to the root of the valve leaflet, of the pipeline support, the T-shaped anchoring structure of the reinforcing rib is embedded into the groove of the pipeline support, and a gap between the valve leaflet and the pipeline support is filled with biological glue; the pipeline support and the outer pipeline are combined and fixed in a physical and chemical mode. The valve leaflet falling risk is reduced, the fatigue life is prolonged, and the reflux and thrombus risks are reduced; due to the nested design of the outer pipeline and the pipeline support, the connecting difficulty between the valve leaflet and the outer pipeline is reduced, and 3D complex design and batch production of the valve leaflet are conveniently achieved.

The invention discloses biological glue and a preparation method thereof.The preparation method comprises the steps that gel and activation liquid are prepared, and the gel is prepared by evenly mixing I-type collagen freeze-dried powder, chitosan and a fibrinogen solution; and mixing the freeze-dried thrombin with a calcium chloride solution to prepare an activation solution. The meninx suturing device has the advantages that sliding in the meninx suturing process is effectively reduced, an operation is easier to carry out, the risk that the meninx or a repairing material slides after the operation can be reduced, and a wound is evenly healed; most of the components are type I collagen which is beneficial to meninx injury repair, and the components contain chitosan which has antibacterial and hemostatic effects.

The invention relates to the technical field of planting equipment, in particular to a green plant transplanter which comprises a cart, the cart comprises a placement plate, a supporting plate is fixedly mounted at the front end of the placement plate, and a mounting plate extending outwards is fixedly mounted at the top end of the supporting plate; a lifting mechanism is mounted on the mounting plate, a clamping mechanism and a lifting mechanism are mounted on the lifting mechanism, the lifting mechanism comprises a second lifting plate, and an excavating mechanism is fixedly mounted on the second lifting plate; the digging mechanism comprises a mounting assembly, and a cutter assembly is mounted in the mounting assembly; a biological glue supply assembly is fixedly mounted on one side of the placement plate, and a telescopic hose is connected between the biological glue supply assembly and the cutter assembly; through cooperative arrangement of the biological glue supply assembly and the cutter assembly in the digging mechanism, a biological glue curing layer can be formed on the surface of a soil ball in real time in the process of forming the soil ball through rotary cutting, the structural integrity of the soil ball can be effectively enhanced, and the survival rate of plants is increased.

The present invention provides a skin in-situ printing device for applying biological glue and a cross-linking agent to a predetermined part of a patient, and has the following characteristics: a platform device for carrying and rotating a patient; a print head device for applying the biological glue and the cross-linking agent to a predetermined part, and having a bracket unit and a nozzle unit arranged on the bracket unit; a coating power device for providing power to squeeze the biological glue and the cross-linking agent out of the nozzle unit during coating; a three-axis moving device for driving the print head device to move to a predetermined part; and a control device for controlling the above-mentioned devices, wherein the nozzle unit has a first nozzle assembly including a first barrel and a first needle and a second nozzle assembly including a second barrel and a second needle, the second barrel and the second needle respectively surround the first barrel and the outside of the first needle and have a first gap and a second gap, the first gap is connected to the second gap, and the lower end of the second needle is lower than the lower end of the first needle.

The present invention discloses a method and system for bio-cementing filling of tailings sand based on bacterial urease. Active, highly fluid, and bio-safety-risk-free bacterial urease is extracted from urease-producing microorganisms, and this urease is used to cement the tailings sand filled in the goaf, which can improve the strength of the goaf filling body. At the same time, to improve the utilization and cementing efficiency of the tailings sand, the tailings sand is screened according to different particle size ranges, and the mixing method and the permeation grouting method are respectively used for filling. The treatment liquid prepared based on bacterial urease has advantages such as good fluidity, high bio-safety level, and environmental friendliness, solving the problem of weak stability that it is difficult or even impossible to repair after the filling body mainly made of cement cracks. At the same time, the solution provided by the present invention can play an excellent role in both anaerobic and aerobic goaf environments, and can also reduce the energy supply and environmental pollution pressure of cement production by reducing the amount of cement used or replacing cement.

The present invention provides a skin in-situ printing device for applying bio-glue and cross-linking agent to a predetermined part of a patient, and has the following characteristics, including: a platform device for carrying and rotating a patient; a print head device having a support unit and a nozzle unit; a storage device including a first storage device and a second storage device; a coating power device for providing coating power to the storage device; a three-axis moving device for driving the print head device to move to a predetermined part; and a control device, wherein the nozzle unit has a first nozzle and a second nozzle, the first nozzle has a first tube portion and a first nozzle head portion, the two ends of the first tube portion are respectively connected to the first storage device and the first nozzle head portion, the second nozzle has a second tube portion and a second nozzle head portion, the two ends of the second tube portion are respectively connected to the second storage device and the second nozzle head portion, the second nozzle extends and passes through the tube wall of the first tube portion, so that the second nozzle head portion is located at the central axis position of the inner cavity of the first tube portion.

The invention relates to biological glue as well as a preparation method and application thereof. The biological glue comprises the following components: tannic acid, gelatin and polyvinylpyrrolidone, the polyvinylpyrrolidone comprises a mixture of polyvinylpyrrolidone K30, polyvinylpyrrolidone K60 and polyvinylpyrrolidone K90, and the polyvinylpyrrolidone K30 is a mixture of polyvinylpyrrolidone K60 and polyvinylpyrrolidone K90. The biological glue has good biocompatibility and high-strength adhesiveness.

The invention provides a biological gel acid liquid system as well as a preparation method and application thereof, and belongs to the technical field of fracturing fluids. The biogel acid liquid system comprises the following components in percentage by mass: 15%-20% of hydrochloric acid, 0.2%-0.8% of biogel, 0.5%-2% of a fluorocarbon discharge aiding agent, 1%-3% of an aldehyde ketone amine condensate corrosion inhibitor, 0.5%-2% of an iron ion stabilizer and the balance of water; wherein the biological glue is a fermentation product of a production strain, and the production strain is sphingomonas sp.WG (sphingomonas sp.WG). Through interaction of the components with specific mass percentage content, the biogel is promoted to form a three-dimensional space network structure in an acid liquid system, so that the acid liquid system has good resistance reduction performance, temperature-resistant stability and low damage performance, and safe, efficient and green carbonate reservoir transformation can be realized.

The invention discloses a self-healing interface binder and a preparation method thereof, and belongs to the technical field of binder preparation. The adhesive is prepared from 90 to 110 parts of water, 8 to 12 parts of absolute ethyl alcohol, 50 to 70 parts of cement, 20 to 30 parts of mineral admixture, 15 to 25 parts of polymerized emulsion, 3 to 7 parts of silane coupling agent, 10 to 15 parts of nano silicon dioxide, 5 to 12 parts of unsaturated amide, 8 to 12 parts of unsaturated acid, 4 to 7 parts of biogel, 5 to 8 parts of thixotropic agent, 3 to 6 parts of dispersing agent and 3 to 6 parts of initiator. The mineral admixture comprises silicon powder and rice hull powder in a mass ratio of 3: 7, the polymerized emulsion is vinyl acetate, the silane coupling agent is KH570, the unsaturated amide is acrylamide, and the unsaturated acid is acrylic acid. According to the interface binder prepared by the preparation method disclosed by the invention, through physical permeation and chemical combination, the bonding strength with concrete can be remarkably improved, and the problems of hollowing and cracking are reduced; in addition, a compact protective layer can be formed on the surface of the interface binder, air and moisture are effectively prevented from invading, and the durability of the concrete is improved.

The invention provides a bamboo joint type umbrella-shaped building structure which comprises a stand column formed by a plurality of supporting rods in a surrounding mode, the stand column can be fixed to a lower body structure or a basic position, and each supporting rod is formed by splicing a plurality of bamboo joint modules; the branch columns are fixed to the stand column and arranged in a radial divergence mode with the stand column as the center to form an umbrella-shaped structure, each branch column is formed by splicing a plurality of bamboo joint modules, a grid is formed between every two adjacent branch columns through reinforcing ribs, and a skylight is arranged in each grid; wherein each bamboo joint module can be independently disassembled and assembled, each bamboo joint module comprises at least one bamboo joint, and splicing joints of every two adjacent bamboo joint modules are connected through mortise and tenon joints or bonded through biological glue. The bamboo joint bionic design is achieved through sectional type bamboo joint module splicing, combination of light weight and high strength is achieved, meanwhile, the mortise and tenon joint or biological glue bonding technology is adopted at the splicing joints, metal connecting pieces are reduced, and integrity is improved.

The invention particularly discloses immunoregulation type bone biological glue as well as a preparation method and application thereof, and relates to the technical field of bone biological glue. The invention provides an immunoregulation type bone biological glue which can regulate polarization, osteogenesis and angiogenic differentiation of macrophages, and further meets the clinical requirements of the bone biological glue for regulating an implant bone surface and interface immune microenvironment and osseointegration.

The invention belongs to the technical field of biological adhesives, and relates to an epoxidized bean dreg modified soybean protein adhesive as well as a preparation method and application thereof. The soybean protein adhesive comprises the following components in parts by weight: 80-120 parts of water, 25-35 parts of soybean meal powder, 10-20 parts of epoxidized soybean dregs, 10-15 parts of a polyamide polyamine-epichlorohydrin resin PAE solution and 1-3 parts of an alkali solution, the epoxidized bean dregs are composed of the following components in parts by weight: 80-120 parts of wet bean dregs, 16-20 parts of epoxy chloropropane, 1-3 parts of aqueous alkali and 300-500 parts of deionized water. Wet bean dregs are subjected to epoxy modification to prepare epoxidized bean dregs, then the epoxidized bean dregs are mixed with PAE resin, soybean meal powder and water, and the soybean protein adhesive with water resistance and bonding strength is prepared. The preparation process is simple, the raw material cost is low, the dependence on a petroleum-based modifier is reduced while the performance of the adhesive is improved, and the preparation method has a wide application prospect.

The present invention provides an amniotic membrane-loaded contact lens, its preparation method, and its application. This invention innovates the bonding method between amniotic membrane and contact lens, replacing the previous method of simply bonding the amniotic membrane and contact lens with medical biological glue. This simplifies the bonding process between amniotic membrane and contact lens, making it suitable for industrial application. It also addresses the issues of traditional sutured amniotic membranes, which suffer from decreased mechanical support, rupture, and uneven coverage over time as they cover the ocular surface. The optimization and selection of materials not only meet the basic requirements of a non-toxic and harmless adhesive, but also take into account biocompatibility and the cornea's requirements for contact lens oxygen permeability. The use of amniotic membranes is more comfortable, eliminating the need for complex surgical procedures and the irritation associated with sutures. The design of using a contact lens as an amniotic membrane support allows amniotic lenses to provide superior support and protection for the covered ocular surface.

The invention relates to the technical field of oil-gas field development, in particular to a modified biogel fracturing fluid thickening agent as well as a preparation method and application thereof, and the biogel fracturing fluid thickening agent is prepared by polymerizing a water-phase mixed solution and an oil-phase mixed solution; wherein the water-phase mixed solution is prepared by mixing the following components in parts by weight: 10 to 20 parts of acrylamide, 10 to 15 parts of methacryloyloxyethyl dimethyl sodium p-benzenesulfonate ammonium chloride, 5 to 8 parts of bis (dodecyl amide) ethylene and 60 parts of water; the oil-phase mixed solution is prepared by mixing 4-8 parts by weight of an emulsifier and 35-45 parts by weight of white oil; the preparation method comprises the following steps: uniformly mixing an oil-phase mixed solution and a water-phase mixed solution, and reacting at 45-65 DEG C for 2-4 hours under the catalysis of azobis (isobutylamidine hydrochloride), the modified biogel fracturing fluid thickener can realize hydrophobic association tackifying in water, has the functions of viscosity change, swelling prevention, resistance reduction and the like, is low in residue, small in damage and good in temperature resistance and shear resistance, and meets the construction requirements.

According to the preparation method of the biological glue, the raw materials comprise a natural polymer and a waterproof additive, the natural polymer adopts soybean protein, gelatin, alginate or starch, and a cross-linking agent (such as glutaraldehyde, melamine formaldehyde and the like) is added, so that the waterproof property of the biological glue is improved. A three-dimensional structure is formed through a catalytic reaction to improve the strength and waterproofness of the glue, additional waterproof treatment is performed on a glue finished product in a soaking or spraying manner, for example, a nano coating or a hydrophilic modifier is used, so that the waterproof effect of the product is further enhanced, the performance of the product can be improved, and the glue can be conveniently applied to various environments; the limitation during use is low, so that the adhesive force and the elasticity can be kept in a humid environment.

The invention belongs to the technical field of flexible intelligent driving materials, and particularly relates to temperature-sensitive biological glue as well as a preparation method and application thereof. The temperature-sensitive biological glue comprises polycaprolactone glycol with the number-average molecular weight of 530 and polycaprolactone glycol with the number-average molecular weight of 2000, has a rapid reversible phase change effect, can be used for sealing a soft micro-robot, can take a good sealing effect and an efficient release effect into account, realizes various scenes such as in-vivo targeted drug delivery and intestinal flora sampling, and has a good application prospect in the fields of in-vivo targeted drug delivery, intestinal flora sampling and the like. Moreover, it can be guaranteed that the contained medicine cannot leak in the delivery process, the sufficient medicine delivery amount is guaranteed, and it is avoided that collected objects leak after sampling, and the collected objects are polluted by other substances in the intestinal tract environment.

The present application relates to a kind of implantable fixation device and method for mouse spinal cord administration, the device includes: catheter assembly, which includes sleeve, injection inner tube and locking nut, the catheter assembly is used to carry out fixed-point infusion in spinal cord parenchyma by the bone hole opened on vertebral vertebra;Vertebra fixation assembly, which includes two V-shaped supports for clamping and fixing on the two sides of the outer periphery of vertebra;And bonding reinforcement system, which includes the initial solid seal layer formed by adhesive to realize the sealing between bone hole and catheter assembly and the structural holding adhesive resin layer formed by resin to realize the fixation between vertebra fixation subassembly and vertebra.The present application designs special vertebra fixation assembly: V-shaped support, and is combined with "biological glue preliminary adhesion" and "dental cement overall wrapping", and constructs a multi-level, rigid-flexible combined "sandwich fixation" composite fixation system.

The application belongs to the technical field of biological adhesives, and particularly relates to a preparation method of a konjac bio-based adhesive and application thereof. The application constructs a high-performance konjac bio-based adhesive through a multi-step aqueous process: firstly, a PVA mother liquor is prepared, high shear is introduced to form a hydrogen bond interpenetrating network with konjac glucomannan, so as to guarantee wettability and early holding force; then, a reversible thixotropic sub-network is constructed by sodium lignosulfonate, tannic acid and kaolin, so as to realize shear thinning and stress buffering; a pre-hydrolyzed silane forms a Si-O-C bridge under weak acid conditions, so as to realize the transition of the interface from physical intercalation to chemical anchoring; a latent crosslinking agent (BTCA / SHP) ensures storage stability, and activates esterification reaction to strengthen the bulk phase when used; silane modified nano-SiO2 forms an inorganic lattice, which cooperates with energy consumption to inhibit cracks; finally, a dense glue line is obtained through vacuum degassing and filtration.

The present invention relates primarily to the use of a mixture for reducing dust formation and / or erosion. The invention relates additionally to a method for reducing dust formation and / or erosion and also to a mixture suitable for this purpose.

The invention relates to a room-temperature collagen mineralization material and a preparation method and application thereof, and the preparation method comprises the following steps: pretreating a fish bubble material to obtain treated fish bubbles; and soaking the treated fish bladders in a mineralization solution for mineralization treatment, and drying to obtain the room-temperature collagen mineralization material. Biological glue can also be used as a binder, and the room-temperature collagen mineralization material is subjected to lamination bonding and compression molding to obtain a block-shaped mineralization collagen material. The fish vesicles are used as collagen materials for mineralization, the fish vesicles are easy to obtain and low in price, the size of the materials is large, the collagen fibers are easy to process, the arrangement fineness of the collagen fibers is high, and the mineralized materials have good mechanical performance; meanwhile, the block-shaped mineralized collagen material with relatively good mechanical properties is prepared through lamination bonding and compression molding.

The invention relates to the technical field of lithium ion batteries, in particular to a preparation method and application of a biological gum-based binder for a lithium ion battery. The method comprises the following steps: (1) uniformly mixing biogel and organic acid; and (2) uniformly mixing the mixture obtained in the step (1) with a dispersing agent, carrying out esterification reaction, and then carrying out drying treatment to obtain the biogum-based binder. According to the invention, the biogel which is low in cost and easy to industrialize is used as a raw material and is subjected to esterification modification with the organic acid, so that the biogel has elasticity and tensile properties. When the biogum-based binder is used as a lithium battery binder, the problem that the cost of the lithium battery binder is high is solved, the affinity of the biogum binder and an electrode material can be effectively improved due to the existence of ester bonds, meanwhile, the water absorption of the binder can be reduced, and the biogum-based binder can be widely used as a positive and negative electrode binder of a battery; and the application prospect of the biogel in the lithium battery binder is widened.

The invention discloses a preparation method of biological glue with a layer-by-layer progressive function, which comprises the following steps: 1) uniformly mixing a silk fibroin solution and tadpole-shaped silk fibroin particles, adding a tannic acid solution, and mixing to form a viscous gel material; and 2) adding a cartilage matrix material / bone repair material into the viscous gel material prepared in the step 1), and uniformly mixing to obtain the biological glue. According to the invention, a reactant formed by the silk fibroin and the tannic acid has strong adhesion in an articular cavity wetting state, and rapid adhesion is realized; meanwhile, a viscous gel material formed by the silk fibroin, the tannic acid and the tadpole-shaped silk fibroin particles provides a three-dimensional environment for cell proliferation, so that the functions of adhesion, migration, differentiation, proliferation and the like of related cells are collected favorably, and the extrinsic repair effect of cartilage is realized; the biological glue is mainly used in the field of osteochondral injury repair, and the function of repairing osteochondral layer by layer is realized by adopting a layer-by-layer injection and rapid adhesion technology.