183 results about "Urease" patented technology

The invention discloses a urease-producing microorganism induced calcium carbonate deposition solution for foundation reinforcement and a preparation method and application thereof, and belongs to the technical field of foundation reinforcement. The urease-producing microorganism induced calcium carbonate deposition solution comprises a bacterial solution, a cementing solution and a sodium citrate solution, the bacterial liquid comprises bacteria capable of generating urease and citrobacter freundii. Sodium citrate in the urease-producing microorganism induced calcium carbonate deposition solution can chelate free calcium ions in advance, the concentration of the free calcium ions is reduced, the risk of precipitation at an injection port in the single-phase injection process is eliminated, and the solution can smoothly permeate into a deep layer; citrobacter freundii produces acid through metabolism and utilizes citric acid radicals through metabolism to ensure gradual release of calcium ions, so that uniform deposition of calcium carbonate is triggered. Through the synergistic effect of sodium citrate chelated calcium ions and citrobacter freundii metabolism, the solution can be uniformly distributed in the soil sample, and the problem of uniformity of single-phase perfusion is successfully solved.

The invention discloses a grouting anchor rod suitable for microorganism induced mineralization reinforcement and an application method of the grouting anchor rod. The grouting anchor rod comprises a sleeve, an anchor head, a hollow stop-grouting plug, an arch-shaped base plate and a nut, and the anchor head is detachably installed at the anchoring end of the sleeve. Through combination of the sleeve and the hollow anchor rod, grouting channels which work independently and do not interfere with each other are formed, grouting materials and biological nutrient solutions containing urease-producing flora are injected into drill holes, pore water around anchor holes and the injected nutrient solutions are used for inducing calcium carbonate to precipitate in an anchoring area, CaCO3 crystals are deposited on an anchoring interface, and the anchoring effect is achieved. Pores are filled, the bonding strength of slurry and rock soil is improved, an anchoring layer is effectively prevented from being stripped, and the interface bonding performance is enhanced; a microorganism induced mineralization technology and an anchor rod physical reinforcement system are combined, and structure-biology-environment multi-scale collaborative reinforcement is achieved; and macrostructure stability and microcosmic particle consolidation are unified, and the enhanced, lasting, stable and eco-friendly slope supporting effect is achieved.

The invention discloses a method for enhancing the biological nitrogen fixation capacity of soil based on micro-plastics, which comprises the following steps: adding poly (adipic acid-butylene terephthalate) micro-plastics into target soil, and the addition amount is 0.2-2% of the mass of the soil. The activity of dehydrogenase and urease of rhizosphere microorganisms is activated through the degradation process of the poly (butylene adipate-co-terephthalate) micro-plastic in soil, and microorganism groups with a nitrogen fixation function are specifically enriched, so that the expression of nitrogen fixation genes is up-regulated, the biological nitrogen fixation capacity of the soil is enhanced, and the nitrogen condition of the soil is improved.

The invention provides a preparation method of a debris flow protection engineering self-repairing concrete part by using microorganisms and magnesium oxide, which comprises the following steps: S1, dipping a porous aggregate base material with the particle size distribution of 1-5mm in a urease-producing microbial agent, and enabling urease-producing microorganisms in the urease-producing microbial agent to fully enter pores of the porous aggregate base material; s2, urea and magnesium oxide are added into the urease-producing microbial agent impregnated with the porous aggregate base material to obtain a mixed solution, the mixed solution is maintained for 2-5 days, the self-repairing aggregate with the particle size distribution ranging from 2 mm to 10 mm is obtained, urea, urease-producing microorganisms, magnesium oxide and water react with one another in the maintenance period, a hardened shell wrapping the outer surface of the porous aggregate base material is generated, and the self-repairing aggregate with the particle size distribution ranging from 2 mm to 10 mm is obtained. Urea and urease-producing microorganisms are retained in pores of the porous aggregate base material, and the hardened shell comprises brucite, basic magnesium carbonate and magnesium oxide which is not completely reacted; and S3, mixing the self-repairing aggregate with the debris flow protection material, and curing to obtain the debris flow protection engineering self-repairing concrete piece utilizing the microorganisms and the magnesium oxide.

The application provides a river sludge resource recycling method. First, the river sludge is pumped out by using physical equipment, then the organic matter of the river sludge is decomposed by using solid fermentation of microorganisms combined with bentonite, and heavy metals are degraded, so that the effect of eliminating odor and harmless treatment is achieved. Then, a vacuum dewatering device is arranged in the fermentation box, so that the sludge is gradually dewatered in the fermentation process, and the water content is reduced to below 10%, finally, a cementing liquid containing calcium ions is sprayed on the sludge, and urease-producing microorganisms are induced to generate calcium carbonate precipitation (MICP), so that the sludge forms a certain strength of the raw soil, which can be used on the roadbed. In this process, the bentonite can play the roles of adsorbing bacterial flora, enhancing the MICP effect, fixing heavy metals and assisting fermentation. The method is low in cost, green and environmentally friendly, solves the problem that the sludge is difficult to be applied for a long time, has certain economic value, and is a disposal method worthy of promotion.

This invention relates to a hydrogen-form ZSM-48 molecular sieve, its synthesis method, and its applications. The hydrogen-form ZSM-48 molecular sieve has an average mesopore size of 6 nm to 30 nm, a B / L acid ratio of 0.7 to 3.4, and a specific surface area of ​​100 m². 2 / g~500m 2 / g. The synthesis method of the hydrogen-form ZSM-48 molecular sieve includes the following steps: (1) Under contact conditions, silicon source, aluminum source, template agent and water are mixed evenly to obtain a gel; (2) The gel obtained in step (1) is subjected to hydrothermal crystallization treatment, and then washed, dried and calcined to obtain hydrogen-form ZSM-48 molecular sieve. The template agent in step (1) includes hexamethylammonium hydroxide and urea; preferably, the template agent also includes urease. The synthesis method controls the molecular sieve pore size and B / L acid ratio by introducing urease and using a two-stage hydrothermal treatment method, thereby realizing the control of molecular sieve properties according to the target reaction characteristics, which is beneficial to improving the yield of low-branched isoalkanes and improving the quality of lubricating oil base oil.

This invention relates to a microbial mineralization composite material for inkjet 3D printing and its preparation method. The composite material comprises powder materials and a binder. The powder materials, by weight, include: 70-85 parts magnesium phosphate cement powder, 10-25 parts fine aggregate, 0.5-3 parts a porous protective carrier loaded with urease-producing Bacillus licheniformis spores, 0.05-0.5 parts a nutrient protectant, and 1-5 parts a calcium source. The binder, by weight, includes: 94-98 parts deionized water, urea, 0.02-0.2 parts nutrients, 0.01-0.5 parts a pH adjuster, 0.5-5 parts a viscosity adjuster, and 0.01-0.2 parts a surface tension adjuster. The concentration of urea in the binder is 0.02-0.10 mol / L. This invention solves the problems of existing inkjet 3D printing materials having internal pores, microcracks, and weak interlayer interfaces after molding, limiting the improvement of the mechanical properties of printed parts in the later stages, and the difficulty in adapting the microbial mineralization system to the inkjet 3D printing process.

The present disclosure relates to a novel technique capable of highly efficiently producing a high-concentration preparation of a target substance in a production process for the target substance utilizing a microorganism under anaerobic conditions or microaerobic conditions, wherein a necessary substrate is efficiently supplied to a microbial metabolic pathway involved in the biosynthesis of the target substance and the dilution of the target substance in a reaction solution is suppressed. Specifically, the present disclosure relates to a method for producing a target substance, the method including (q) culturing or reacting cells of a microorganism having a urease activity or a treatment product of the cells in a culture medium or reaction medium (X) under anaerobic conditions or microaerobic conditions to produce the target substance, wherein, during the step (q), a urea-containing solution (Y) is added to the culture medium or reaction medium (X).

The invention discloses a medicine for inhibiting helicobacter pylori infection, the medicine is oridonin, and the structural formula of the medicine is as shown in formula 1. In-vitro experiments show that the oridonin has a remarkable antibacterial effect on helicobacter pylori strains SS1, ATCC700392 and ICDC11101, and can remarkably reduce the urease activity of the helicobacter pylori, inhibit the formation of a biological membrane, destroy the growth of thalli and reduce the adhesion effect of the helicobacter pylori on gastric epithelial cells, and in addition, the oridonin and antibiotics are combined for use, so that the effect of preventing and treating the gastric epithelial cells can be achieved. The compound has a synergistic antibacterial effect with metronidazole and clarithromycin, has the effect of reducing the dosage of antibiotics in clinical application, and has potential application value for improving the current drug resistance situation of the antibiotics.

The application provides a self-driven BCG-nanoparticle complex and a preparation method thereof, and belongs to the fields of biological medicine and nanomaterials. The complex is a composite of BCG and cerium oxide nanoparticles with urease activity, the cerium oxide nanoparticles are coupled to the surface of the BCG in an asymmetric distribution through modified polyethyleneimine, and specifically, the surface of the BCG is connected to several modified polyethyleneimine derivatives through chemical bonds and electrostatic adsorption, and the polyethyleneimine derivatives wrap the cerium oxide nanoparticles through electrostatic adsorption. The complex can be self-driven by using the gas release and ion concentration change caused by the catalytic decomposition of urea in the environment, significantly enhances the adhesion and retention of BCG on the bladder wall, effectively overcomes the defects of low delivery efficiency and insufficient immune activation of traditional BCG therapy, simultaneously improves the tumor immune microenvironment, and can be used for preparing high-efficiency and low-toxicity bladder cancer treatment drugs.

The utility model relates to a soybean urease purification device suitable for a construction site, which comprises a fixed outer barrel, the barrel bottom of the fixed outer barrel is connected with a liquid discharge pipe with an electromagnetic valve, a rotary inner barrel driven by a motor is arranged in the fixed outer barrel, a plurality of water permeable holes are formed in the periphery of the rotary inner barrel, and a filter bag is arranged in the rotary inner barrel. And the filter bag is driven by a driving mechanism to revolve and rotate in the rotary inner barrel. The device is simple in structure, easy to operate and convenient to transport, and urease solution preparation can be conveniently carried out on a construction site. By means of the device, large-scale purification preparation of the urease solution on an engineering site can be achieved, purification of the urease solution is facilitated, the centrifugal purification effect is good, and the efficiency is high.

The invention discloses a device and method capable of dynamically monitoring heavy metal polluted water and soil repaired through an EICP technology, the device comprises a support, three monitoring systems and a grouting system, the first monitoring system comprises a measuring box, a top cover, a motor, a grouting hole, a transmission shaft, a gear, an inner gear ring, a stirring sampling pipe, a supporting rod, a sheet type multifunctional sensor and a sampling opening; the second monitoring system comprises a liquid conveying hose, a flow control valve, a peristaltic pump, a pump pipe, a measuring box, a raw material box, a waste liquid box, a conductivity meter and a magnetic stirrer; the third monitoring system comprises a support frame, a transfusion hose, a flow control valve, a peristaltic pump, a pump pipe, a centrifugal filter, a measuring box, a raw material box, a waste liquid box and an atomic absorption spectrophotometer; the grouting system comprises a urease solution barrel, a urea solution barrel, a three-way pipe and a suction pump. Biological, chemical and physical indexes in the repairing process can be monitored in real time, and the repairing process can be known.

The application discloses a method for promoting growth of cymbidium on a steel slag flat bed based on microbial organic matrix, and belongs to the technical field of steel slag resource utilization. The application inoculates eight kinds of bacillus agents with different concentrations on the bacterial residue to prepare the organic matrix, and then covers the matrix on the converter slag to promote the growth of cymbidium on the converter slag, and selects the bacillus strain with the best growth promotion effect and the most suitable inoculation concentration. The results show that after being inoculated with bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus, the sucrose activity of the soil is enhanced; the hydrogen peroxide activity of the soil in the four groups of treatments inoculated with the bacillus agent is stronger than that in the control group; the application of the bacillus megaterium, bacillus polymyxa and bacillus mucilaginosus enhances the urease activity in the soil; the inoculation of the bacillus megaterium promotes the release of Ca and P elements in the soil and the absorption of Ca, P, Mg, Mn and Si elements by the plant.

The invention provides an algae source soil conditioner for synergistically passivating copper and cadmium as well as a preparation method and application of the algae source soil conditioner, and belongs to the technical field of soil remediation. The algae source soil conditioner comprises an algae source active component and a biochar-based carrier. By combining activity retention of the seaweed extract and functional modification of pyrolytic carbon, the content of copper ions and cadmium ions in soil can be remarkably reduced, meanwhile, the activity of soil urease, sucrase and dehydrogenase is promoted to be remarkably improved, and the synergistic effect of heavy metal passivation and soil ecological function restoration is achieved; the preparation method disclosed by the invention is simple and easy to operate, and the prepared algae source soil conditioner has a multi-stage pore structure and rich surface functional groups.

The invention relates to the field of acid soil conditioners, in particular to a charcoal-based acid soil conditioner as well as a preparation method and application thereof. The biochar-based acid soil conditioner comprises a biochar carrier and a complex microbial inoculant loaded on the biochar carrier, and the complex microbial inoculant comprises 25-35 parts of nitrogen-fixing functional bacteria; 20 to 40 parts of phosphorus and potassium solubilizing bacteria; 15 to 25 parts of nitrogen activating bacteria; 15 to 25 parts of synergistic growth promoting bacteria; the nitrogen-fixing functional bacteria comprise Azospirillum brasilense and / or Klebsiella, the phosphate-solubilizing and potassium-solubilizing bacteria comprise bacillus megatherium and / or bacillus mucilaginosus, the nitrogen-activating bacteria comprise urease-producing bacillus and / or nitrospirillum, and the synergistic growth-promoting bacteria comprise arbuscular mycorrhizal fungi and / or pseudomonas. The complex microbial inoculant is constructed by screening four functional strains of nitrogen fixation, phosphorus and potassium solubilization, nitrogen activation and synergistic growth promotion, a complete functional closed loop of nitrogen fixation, phosphorus solubilization, nutrient activation and crop growth promotion is formed, and synergistic interaction is achieved through functional complementation of the strains.

The application discloses a preparation method of a high-efficiency bio-mineralized recycled construction waste mixture under load action, adopts a hydrostatic pressure loading reactor, carries out gradient hydrostatic pressure domestication on a starting strain, and obtains a high-load-resistant strain; the high-load-resistant strain is cultured in a large scale to obtain a high-pressure-resistant bacterial liquid; soluble calcium salt, urease activator, crystal form regulator and water are mixed to prepare a composite inducer; the high-pressure-resistant bacterial liquid and the composite inducer are uniformly mixed according to a volume ratio to obtain a mineralization mixing liquid; recycled construction waste aggregates and cementing materials are weighed and put into a stirring device, the mineralization mixing liquid is added into the stirring device as mixing water to be stirred, and pressure forming and curing are carried out after uniform stirring to obtain the recycled construction waste mixture. Through directional screening of the pressure-resistant strain and preparation of a rapid induction system, the application solves the problems of inactivation of microorganisms and low mineralization efficiency under extrusion load, and the aggregates do not need to be pretreated and can be directly integrated into the existing engineering mixing process.

The application relates to the technical field of agricultural analysis and detection, and discloses a multi-union type fertilizer synergist urease activity automatic detection and analysis system, which comprises a physical execution layer and an intelligent control layer. The physical execution layer is configured with a reaction generation module, a gas transmission and conditioning module and a detection and regeneration module which are connected in sequence in a gas path, is used for constructing a closed loop flow path and executing ammonia gas absorption and titration. The intelligent control layer controls the gas transmission and conditioning module to switch the gas path according to an adaptive time sequence, constructs an intermittent absorption environment, executes static titration and resets trace acid liquid under the condition of maintaining a bypass state, and simultaneously uses system lag parameters to perform dynamic deconvolution reconstruction on discrete titration data collected, so as to restore a continuous reaction rate curve. Through the combination of hardware time sequence control and algorithm correction, the application eliminates the influence of physical transmission delay on the signal phase, and improves the detection precision and efficiency of the dynamic change of urease activity.

The invention discloses a phosphate-solubilizing bacterium with dual functions of phosphate solubilizing and passivation, a microbial agent prepared from the phosphate-solubilizing bacterium and application of the phosphate-solubilizing bacterium, the phosphate-solubilizing bacterium is Bacillus sp and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the phosphate-solubilizing bacterium is GDMCC NO: 66609. Through multiple rounds of screening, the strain capable of dissolving phosphorus, producing urease, IAA and siderophore, promoting corn growth and improving cadmium and lead tolerance of plants is obtained, the strain can greatly improve underground biomass of corn, meanwhile, urease, IAA and siderophore are produced, effectiveness of soil cadmium is reduced, and soil fertility is improved. The fertilizer can increase the yield of corn, improve the soil fertility and improve the market competitiveness of agricultural products. The phosphate-solubilizing bacterium disclosed by the invention is easy to culture and apply on a large scale, simple and convenient to operate, easy to accept and apply by agricultural producers, and relatively high in practicability. The strain and the preparation have relatively high agricultural application prospects and economic values.

The present application relates to the technical field of agricultural breeding, and discloses a large-scale cage feeding method for low-nitrogen emission broiler chickens, which comprises a nutrition regulation module, a feeding environment management module, a harmless and resourceful treatment module for manure, and an intelligent monitoring module. The present application constructs a whole-chain low-nitrogen emission system from "feed source, feeding process, and end treatment". This systematic integration ensures that nitrogen is effectively controlled at each link, and minimizes ammonia emission and nitrogen pollution. The present application directly reduces about 15-20% of nitrogen intake and excretion by using low-protein daily feed and precise amino acid balance. In combination with the inhibition of urease by additives such as yucca extract, the purpose of controlling ammonia pollution from the source is achieved.

The invention relates to the technical field of self-healing concrete, in particular to fiber-reinforced self-healing concrete and a preparation method thereof. The fiber-reinforced self-healing concrete is prepared from the following raw materials in parts by mass: 5 to 10 parts of silica sol, 1 to 2 parts of calcium sulfate, 10 to 20 parts of ethylene-vinyl acetate copolymer, 5 to 15 parts of TPE resin, 0.1 to 1 part of sodium chloride, 1 to 3 parts of urease bacteria liquid, 1 to 3 parts of dopamine hydrochloride, 40 to 60 parts of coarse aggregate, 20 to 40 parts of fine aggregate, 1 to 5 parts of chopped basalt fiber, 5 to 15 parts of kaolin and 1 to 4 parts of urea. 40-60 parts of fly ash, 40-50 parts of cement and 1-2 parts of a water reducing agent. According to the invention, the strength and durability of the concrete are effectively improved, and even if the concrete structure is locally damaged and leaked, the concrete structure can be automatically repaired and healed, so that cracking and seepage caused by unstable volume of a common concrete structure are essentially changed.

The invention relates to the technical field of geological reinforcement, and discloses a grout stopping method for stratum pre-grouting reinforcement, which adopts an A and B double-component grout system: the B component contains thermal stability urease inhibited by a reversible inhibitor; the component A contains a corresponding inhibitor and a corresponding scavenger, and the two components are mixed to release the reaction through inhibition, so that the controllable prolonging of the slurry gelation time is realized, and the blockage of a pipeline is avoided. A self-indication and slow-release carrier loaded with copper ions is arranged in the component A, after the slurry reaction is started, ammonium ions of a reaction product replace the copper ions, the blue change of the slurry is caused, and a visual basis is provided for judging the injection stopping opportunity. The component A further comprises an energy field response medium and reserve urea packaged in the carrier, and the energy field response medium and the reserve urea enable the consolidation body to have the later non-contact remote control enhancement capacity and the micro-crack passive self-repairing capacity respectively. The grouting process is safe and controllable, the reaction state is visible in real time, and the performance of the consolidated body is regulated and controlled in the later period.

The embodiment of the present specification provides an antibody or antigen binding fragment binding to Helicobacter pylori antigen protein urease, the antibody or antigen binding fragment comprising a heavy chain variable region and a light chain variable region. Wherein, the heavy chain variable region comprises CDRH1 of an amino acid sequence as shown in SEQ ID NO: 2, CDRH2 of an amino acid sequence as shown in SEQ ID NO: 3 and CDRH of an amino acid sequence as shown in SEQ ID NO: 4. The light chain variable region comprises CDRL1 of an amino acid sequence as shown in SEQ ID NO: 6, CDRL2 of an amino acid sequence of DTS and CDRL3 of an amino acid sequence as shown in SEQ ID NO: 7. The antibody or antigen binding fragment has the characteristics of high purity, high specificity and sensitivity. The embodiment of the present specification also provides related application of the antibody or antigen binding fragment binding to Helicobacter pylori antigen protein urease.

The invention relates to the technical field of microorganisms, in particular to a metagen for inhibiting helicobacter pylori infection and a preparation method of the metagen. The metagen is prepared by culturing the plant lactobacillus HYProbio-Y4, the preservation number of the plant lactobacillus HYProbio-Y4 is CGMCC (China General Microbiological Culture Collection Center) No.35968, and the metagen has the capability of inhibiting the activity of helicobacter pylori and urease in vitro; and the urease inhibitor can tolerate extreme temperature and pH, and can maintain high urease inhibition activity under the conditions that the temperature is 37-60 DEG C and the pH is 2-11.

The invention discloses a methylobacterium DDY5, a microbial inoculum thereof and application of the methylobacterium DDY5 in improving the yield and quality of solanaceae crops. The methylobacterium DDDY5 is classified and named as Methylobacterium sp., and the preservation number of the methylobacterium DDDY5 is CGMCC (China General Microbiological Culture Collection Center) No.36116. A bacterial colony of the methylobacterium DDY5 is orange red, round and neat in edge, and the surface of the bacterial colony is in a film shape. The methylobacterium DDY5 has the capacity of synthesizing gibberellin and secreting urease, and the methylobacterium DDY5 and the microbial inoculum thereof can obviously promote the growth of tomato seedlings and improve the yield and quality of tomatoes, and have important significance for protecting the farming environment.

The invention belongs to the technical field of fertilizer synergism, and particularly relates to a copper-based coordination polymer, a preparation method and application in nitrogen fertilizer synergism, and the method comprises the following steps: synthesizing N1, N4-bis (3-pyridyl)-2-butene amide by using 3-aminopyridine and fumaric acid as raw materials; and synthesizing the N1, N4-bis (3-pyridyl)-2-buteneamide, CuCl2. 2H2O and 5-nitroisophthalic acid into a new copper-based coordination polymer [Cu (3-bbpa) (5-NIPA)]. H2O by using a hydrothermal method, so as to obtain a blue fine crystal. The copper-based coordination polymer prepared by the invention can synchronously target a urease active center and a nitrifying microorganism functional unit, and a three-dimensional network structure of the copper-based coordination polymer not only enhances the stability of a material in soil, but also realizes double blocking of nitrogen conversion key enzyme through a multi-site synergistic effect.

The invention belongs to the technical field of geotechnical engineering, particularly relates to the technical field of soil remediation, and particularly relates to a soil heavy metal curing composition and an application method thereof. The composition is prepared from the following raw materials: a bean urease aqueous solution, a chitosan aqueous solution, urea and inorganic acid, and does not contain exogenous calcium salt. Aiming at the problems of heavy metal complexing migration and secondary activation caused by high pH and addition of exogenous calcium ions in the existing enzyme induced carbonate precipitation (EICP) technology, the pH value of a reaction system is adjusted to 7.5-8.3 by introducing inorganic acid, and efficient solidification of the heavy metal is realized under the calcium-free condition by using the chelation of chitosan. According to the method, soil salinization and hardening are avoided, the bio-availability and migration risk of heavy metal are remarkably reduced, meanwhile, the soil moisture content is controlled, subsequent dehumidification treatment is not needed, the engineering cost is reduced, and the method is particularly suitable for heavy metal pollution treatment of complex soil environments such as humus soil.

The invention relates to the technical field of helicobacter pylori detection, and discloses a method for acquiring exhaled ammonia gas variation, which comprises the following steps: S1, acquiring exhaled gas before a subject takes a urea reagent at a first time point, and measuring to obtain a basic concentration value C0 of endogenous ammonia gas; s2, enabling the subject to take a reagent containing urea and an alkaline substance; s3, obtaining expired gas after the subject takes the reagent, and measuring to obtain the concentration value C1 of the ammonia gas. According to the invention, a subject takes the reagent containing urea and alkaline substances, so that the pH value in the stomach is remarkably increased, and ammonia gas is inhibited from reacting with gastric acid to generate ammonium ions, thereby increasing the proportion of ammonia gas generated by decomposing urea by helicobacter pylori urease into exhaled gas and enhancing a detection signal. By comparing the variable quantity of ammonia gas concentration in expired gas before and after medicine taking and combining with a preset age adaptability threshold value, high-sensitivity and high-specificity judgment on helicobacter pylori infection is realized.

The invention provides a self-driven bacillus calmette-guerin vaccine-nano enzyme compound and a preparation method thereof, and belongs to the field of biological medicines and nano materials. The compound is a compound of bacillus calmette-guerin and cerium oxide nanoparticles with urease activity, the cerium oxide nanoparticles are coupled to the surface of the bacillus calmette-guerin through modified polyethyleneimine and are asymmetrically distributed, specifically, the surface of the bacillus calmette-guerin is connected with a plurality of modified polyethyleneimine derivatives through chemical bonds and an electrostatic adsorption effect, and the modified polyethyleneimine derivatives are coupled to the surface of the bacillus calmette-guerin. The cerium oxide nano particles are wrapped by the polyethyleneimine derivative through an electrostatic adsorption effect. The compound provided by the invention can realize self-driving by utilizing gas release and ion concentration change caused by urea catalytic decomposition in an environment, remarkably enhances the adhesion and retention of BCG on a bladder wall, effectively overcomes the defects of low delivery efficiency and insufficient immune activation of a traditional BCG therapy, improves a tumor immune microenvironment, and improves the tumor immune activity. The compound can be used for preparing high-efficiency and low-toxicity bladder cancer treatment medicines.