264 results about "Urease" patented technology

The invention relates to a coral sand soil multi-dimensional ecological low-carbon reinforcement method which comprises the following steps: S1, a urease solution is sprayed on a coral sand single-particle soil layer, a cementing liquid A is sprayed after standing, the urease solution is extracted from legume plants, and the cementing liquid A is obtained by mixing a urea solution and a calcium chloride solution; s2, on the basis of treatment in the step S1, a sporosarcina pasteurii bacterial solution is firstly sprayed, after standing, a cementing solution B is sprayed, the cementing solution B is alternately and circularly sprayed for multiple times, and the cementing solution B is obtained by mixing a urea solution and a calcium chloride solution; s3, a 3D printing geosynthetic material is laid on the basis treated in the step S2; and S4, laying a coral sand single-particle soil layer on the basis of treatment in the step S3, and repeating the steps S1 to S3. According to the method, a single particle size-foundation unit size-engineering size reinforcing system is constructed, comprehensive reinforcing from a microscopic single particle to a macrostructure can be achieved, and the reinforcing effect and efficiency are effectively improved.

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

The invention discloses a dialysate regeneration system and dialysis equipment, the dialysate regeneration system comprises a waste liquid passage, a slow flow box, an electromagnetic valve, a reactor and an adsorption mechanism, the waste liquid passage is in through connection with the slow flow box, the slow flow box is connected with the reactor through a pipeline, and the pipeline is provided with the electromagnetic valve. The fixing amount of a urease layer is increased through the treatment film with the three cambered surfaces, the urea decomposition efficiency is improved, the diffusion path of toxins on the treatment film is shortened, meanwhile, the treatment film with the cambered surface structure can guide dialysis waste liquid entering the reactor to generate local turbulence, and the toxins are prevented from being accumulated on the surface of the treatment film; the dialyzer is arranged, the micro horse is started to drive the hollow fiber to rotate, the material exchange efficiency of blood and dialysate is improved, and the situation that protein and cells are deposited on the surface of the hollow fiber, and the service life of the hollow fiber is shortened is avoided.

The invention relates to an integrated grouting device and an offshore wind power single pile foundation anti-scour construction method based on an indigenous microorganism precuring seabed, and the method comprises the following steps: prefabricating a special integrated grouting device according to the installation condition of an offshore wind power single pile foundation; an integrated grouting device is installed on the seabed of the to-be-constructed area through a hammering method and a negative pressure method; urease microorganisms are enriched and cultivated in in-situ seabed soil through an integrated grouting device, the microorganisms are induced to generate calcium carbonate gelatinization crystals so as to bond sandy soil particles, and the overall strength of a surface seabed is improved; and a single-pile foundation is installed on the microorganism solidification seabed. Before pile sinking construction, the surface seabed of the to-be-constructed area is intensified in advance through in-situ enrichment cultivation of indigenous urease microorganisms and induction of mineralization and crystallization of the microorganisms, scouring protection of the single pile foundation in the whole service cycle is achieved, and then the local scouring problem in the pile sinking process is solved.

The invention provides an application of a lactobacillus paracasei IOB413 metagen in prevention of helicobacter pylori infection, and relates to the technical field of microorganisms. The lactobacillus paracasei IOB413 metaplasm can competitively occupy gastric epithelial cell adhesion sites to directly block the initial colonization of helicobacter pylori, so that infection preposed interception is realized, and the lactobacillus paracasei IOB413 metaplasm is suitable for early prevention of high-incidence people. The compound has dual mechanisms of site blocking and urease inhibition, and can cooperate with antibiotics to improve the eradication rate and reduce the drug resistance. Animal experiments show that the traditional Chinese medicine composition can reduce gastric mucosa proinflammatory factors in a dose-dependent manner, improve the activity of antioxidant enzymes, reduce lipid peroxidation products, relieve mucosa lesion, promote repair and reduce the risk of complications; moreover, gastrointestinal hormone disorder can be reversed, the digestive function is maintained, and related discomfort is relieved.

The invention provides an EICP-based sandy soil curing agent, a sandy soil curing method and application thereof, and relates to the technical field of rock and soil reinforcement. The sandy soil curing agent comprises a urease solution, a cementing fluid and a hydrophobic polymer, wherein the urease solution is prepared by salting out a urease crude extract and then carrying out solid-liquid separation; the cementing liquid comprises calcium salt, magnesium salt and urea. The curing agent is convenient to use, the solidified soil body is stable in structure, and the curing agent has excellent disintegration resistance. The invention further provides a sandy soil curing method and application of the sandy soil curing agent or the sandy soil curing method.

The invention discloses a urease-producing microorganism induced calcium carbonate deposition solution for foundation reinforcement and a preparation method and application thereof, and belongs to the technical field of foundation reinforcement. The urease-producing microorganism induced calcium carbonate deposition solution comprises a bacterial solution, a cementing solution and a sodium citrate solution, the bacterial liquid comprises bacteria capable of generating urease and citrobacter freundii. Sodium citrate in the urease-producing microorganism induced calcium carbonate deposition solution can chelate free calcium ions in advance, the concentration of the free calcium ions is reduced, the risk of precipitation at an injection port in the single-phase injection process is eliminated, and the solution can smoothly permeate into a deep layer; citrobacter freundii produces acid through metabolism and utilizes citric acid radicals through metabolism to ensure gradual release of calcium ions, so that uniform deposition of calcium carbonate is triggered. Through the synergistic effect of sodium citrate chelated calcium ions and citrobacter freundii metabolism, the solution can be uniformly distributed in the soil sample, and the problem of uniformity of single-phase perfusion is successfully solved.

To provide a soil improvement method utilizing urea and urease, allowing reduction of ammonia generated in the course of soil improvement.SOLUTION: The present invention provides a soil improvement method in which calcium carbonate is generated from carbon dioxide produced through urea and urease to solidify soil, the method characterized by further adding a phosphorus compound and a magnesium compound to produce magnesium ammonium phosphate from at least a part of the ammonia generated through urea and urease.SELECTED DRAWING: None

The invention provides a strain of Priestia megaterium M23 and application thereof, belongs to the technical field of agricultural microbial strains, and particularly provides a strain of Priestia megaterium M23 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20232384, and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384 and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384. The strain Pristia megaterium M23 provided by the invention has high salt tolerance and drought tolerance, has the function of promoting plant growth, improves the salt tolerance and drought tolerance of plants, can improve the soluble sugar content of the plants, increases the abundance of soil microorganisms, increases the urease activity of the soil, improves the quality of crop seeds and the like, and has important application value.

The invention discloses a grouting anchor rod suitable for microorganism induced mineralization reinforcement and an application method of the grouting anchor rod. The grouting anchor rod comprises a sleeve, an anchor head, a hollow stop-grouting plug, an arch-shaped base plate and a nut, and the anchor head is detachably installed at the anchoring end of the sleeve. Through combination of the sleeve and the hollow anchor rod, grouting channels which work independently and do not interfere with each other are formed, grouting materials and biological nutrient solutions containing urease-producing flora are injected into drill holes, pore water around anchor holes and the injected nutrient solutions are used for inducing calcium carbonate to precipitate in an anchoring area, CaCO3 crystals are deposited on an anchoring interface, and the anchoring effect is achieved. Pores are filled, the bonding strength of slurry and rock soil is improved, an anchoring layer is effectively prevented from being stripped, and the interface bonding performance is enhanced; a microorganism induced mineralization technology and an anchor rod physical reinforcement system are combined, and structure-biology-environment multi-scale collaborative reinforcement is achieved; and macrostructure stability and microcosmic particle consolidation are unified, and the enhanced, lasting, stable and eco-friendly slope supporting effect is achieved.

A chitosan-heparin nanomotor and a method for producing same are disclosed. A STING agonist-encapsulated urease-based chitosan-heparin nanomotor delivers the STING agonist directly to bladder mucosal cells in the bladder, and thus can induce an immune response.

The invention relates to a heavy metal polluted soil repairing and reinforcing method by utilizing indigenous microorganisms in cooperation with CO2 carbonization, which comprises the following steps: firstly, measuring indexes of heavy metal polluted soil, stacking the heavy metal polluted soil in different areas, and collecting specific polluted soil to test microbial indexes; preparing a polluted soil-urease bacterium suspension, and culturing to form a suspension; preparing an indigenous urease bacterium solution; preparing an indigenous urease bacterium liquid-urea mixed solution; then polluted soil is pre-repaired, a curing agent and the mixed solution are mixed, and particles are prepared and cured; and finally, high-concentration CO2 is introduced for reinforced carbonization repair, and the improved soil meeting the requirements is obtained. According to the method, indigenous microorganisms and CO2 carbonization are fully utilized, the method is especially suitable for repairing and reinforcing heavy metal polluted soil containing lead, copper, zinc and the like, the problems that a traditional solidification and stabilization method is high in energy consumption and poor in economical efficiency, and an existing microorganism mineralization technology is poor in microorganism adaptability and poor in repairing effect are solved, and the method is suitable for large-scale popularization and application. The method has the advantages of simple operation process, economy, effectiveness, low carbon and environmental protection.

A ground property adjustment method characterized by: solidifying the ground by proliferating microorganisms, in the ground, that are capable of producing a biological membrane (biofilm), have urease activity, and can cause calcium ions to precipitate as calcium carbonate through decomposition of urea by the urease activity. Microorganisms may precipitate calcium carbonate on the surface of a biological membrane by incorporating carbon dioxide in the environment.

The invention relates to the field of ocean pile foundation maintenance, and discloses a construction method for reinforcing a soil body around a pile based on microbial capsules and a reinforcing structure. The construction method comprises the following steps: culturing urease-producing bacteria; the method comprises the following steps: mixing a first water-soluble capsule for encapsulating cementing powder, a second water-soluble capsule for encapsulating bacterial sludge and graded broken stone and grit to form a mixture; and the mixture is placed in an expected reinforcement area around the pile. Bacterial sludge and cementation powder are packaged through two kinds of capsules respectively and mixed with graded broken stone, then the mixture is put around a pile, the capsules are supplemented regularly, and microorganism-induced mineralization reinforcement is achieved. According to the reinforcement method combining the microbial capsules with the gravel, the anti-scouring capacity of the soil around the pile can be effectively enhanced through the calcium carbonate deposition reaction generated by microorganisms, and compared with a traditional physical protection and chemical reinforcement method, better environmental adaptability and sustainability are achieved; and salt erosion and pollution of a traditional chemical grouting material are avoided.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

According to the method for improving solidification of the aeolian sandy soil by constructing the synthetic community based on the composite urease bacteria, the composite urease bacteria are screened based on collection and treatment of local aeolian sandy soil and strains to construct the synthetic community, the composite urease bacteria liquid is sprayed on the aeolian sandy soil, and the composite flora can improve the deposition rate and quality of calcium carbonate through a synergistic effect, so that the solidification of the aeolian sandy soil is improved. Meanwhile, the adaptability to environment change is enhanced, so that the aeolian sandy soil is effectively solidified; calcium carbonate deposition and solidification of aeolian sandy soil are carried out through a composite urease bacterial solution, and the method comprises the following steps: step 1, sample collection and pretreatment; step 2, primarily screening and separating urease-producing strains; 3, performing urease activity quantitative detection and secondary screening; 4, testing the extreme environment adaptability; step 5, preparing a composite urease bacterium community; and step 6, preparing a composite urease bacterial liquid.

The invention discloses a method for enhancing the biological nitrogen fixation capacity of soil based on micro-plastics, which comprises the following steps: adding poly (adipic acid-butylene terephthalate) micro-plastics into target soil, and the addition amount is 0.2-2% of the mass of the soil. The activity of dehydrogenase and urease of rhizosphere microorganisms is activated through the degradation process of the poly (butylene adipate-co-terephthalate) micro-plastic in soil, and microorganism groups with a nitrogen fixation function are specifically enriched, so that the expression of nitrogen fixation genes is up-regulated, the biological nitrogen fixation capacity of the soil is enhanced, and the nitrogen condition of the soil is improved.

The invention provides a preparation method of a debris flow protection engineering self-repairing concrete part by using microorganisms and magnesium oxide, which comprises the following steps: S1, dipping a porous aggregate base material with the particle size distribution of 1-5mm in a urease-producing microbial agent, and enabling urease-producing microorganisms in the urease-producing microbial agent to fully enter pores of the porous aggregate base material; s2, urea and magnesium oxide are added into the urease-producing microbial agent impregnated with the porous aggregate base material to obtain a mixed solution, the mixed solution is maintained for 2-5 days, the self-repairing aggregate with the particle size distribution ranging from 2 mm to 10 mm is obtained, urea, urease-producing microorganisms, magnesium oxide and water react with one another in the maintenance period, a hardened shell wrapping the outer surface of the porous aggregate base material is generated, and the self-repairing aggregate with the particle size distribution ranging from 2 mm to 10 mm is obtained. Urea and urease-producing microorganisms are retained in pores of the porous aggregate base material, and the hardened shell comprises brucite, basic magnesium carbonate and magnesium oxide which is not completely reacted; and S3, mixing the self-repairing aggregate with the debris flow protection material, and curing to obtain the debris flow protection engineering self-repairing concrete piece utilizing the microorganisms and the magnesium oxide.

The invention relates to the technical field of urease-induced calcium carbonate precipitation grouting, in particular to an integrated SIP monitoring and electric EICP grouting device and a control method thereof.The control method comprises the steps that the polarity of a plurality of rod-shaped electrodes is determined, and EICP reactants are injected into low-permeability soil; starting a strong electric field of the plurality of rod-shaped electrodes to drive electroosmosis and electromigration to realize directional transportation of the EICP reactant in the low-permeability soil body and form an acidic migration boundary and an alkaline migration boundary; closing the strong electric fields of the plurality of rod-shaped electrodes according to a preset time-sharing work and measurement mechanism, and collecting current calcium carbonate precipitation distribution diagrams of different rod-shaped electrodes by using a plurality of SIP monitoring devices; and generating an injection control sequence according to the current calcium carbonate precipitation distribution diagram, and iteratively executing injection, acquisition and generation processes according to the injection control sequence until target calcium carbonate precipitation distribution is formed. Therefore, the problems of difficult transportation, out-of-control reaction, no monitoring and the like existing in a traditional pressure grouting method are solved.

The invention relates to a method for relieving continuous cropping obstacles of ginseng and application of the method, and belongs to the technical field of agricultural planting. The method comprises the following specific steps: planting wrinkled gianthyssop herb on a field after ginseng planting, crushing the wrinkled gianthyssop herb, returning the crushed wrinkled gianthyssop herb to the field, and improving soil for subsequent ginseng planting. The method can effectively relieve continuous cropping obstacles of ginseng, increase the content of soil organic matter, nitrogen, phosphorus and potassium, adjust the pH of soil, improve the activity of soil catalase, reduce the activity of sucrase and urease, optimize the microbial community structure of soil, reduce the abundance of pathogenic bacteria and reduce the incidence rate of root rot and rust rot of ginseng; the yield and quality of ginseng are remarkably improved, the ginsenoside content is increased, meanwhile, use of chemical fertilizers and pesticides is reduced, a green and safe planting mode is achieved, and remarkable economic benefits and ecological benefits are achieved.

The application provides a river sludge resource recycling method. First, the river sludge is pumped out by using physical equipment, then the organic matter of the river sludge is decomposed by using solid fermentation of microorganisms combined with bentonite, and heavy metals are degraded, so that the effect of eliminating odor and harmless treatment is achieved. Then, a vacuum dewatering device is arranged in the fermentation box, so that the sludge is gradually dewatered in the fermentation process, and the water content is reduced to below 10%, finally, a cementing liquid containing calcium ions is sprayed on the sludge, and urease-producing microorganisms are induced to generate calcium carbonate precipitation (MICP), so that the sludge forms a certain strength of the raw soil, which can be used on the roadbed. In this process, the bentonite can play the roles of adsorbing bacterial flora, enhancing the MICP effect, fixing heavy metals and assisting fermentation. The method is low in cost, green and environmentally friendly, solves the problem that the sludge is difficult to be applied for a long time, has certain economic value, and is a disposal method worthy of promotion.

The invention relates to a 14C urea pharmaceutical composition for helicobacter pylori diagnosis and application of the 14C urea pharmaceutical composition. The 14C urea pharmaceutical composition comprises 14C urea, 12C urea, citric acid, malic acid and mannitol. According to the invention, the 12C urea and the 14C urea are added together, so that the radioactivity can be ensured, the stability of the medicine can be improved, and the auto-radiolysis of 14C can be reduced. Besides, citric acid, malic acid and mannitol have a remarkable synergistic effect in the aspect of improving the radioactive activity and radiochemical purity of the composition, the stability of the 14C urea pharmaceutical composition can be improved, meanwhile, the activity of urease generated by helicobacter pylori can be improved, and the detection sensitivity and detection efficiency are further guaranteed.

This invention relates to a hydrogen-form ZSM-48 molecular sieve, its synthesis method, and its applications. The hydrogen-form ZSM-48 molecular sieve has an average mesopore size of 6 nm to 30 nm, a B / L acid ratio of 0.7 to 3.4, and a specific surface area of ​​100 m². 2 / g~500m 2 / g. The synthesis method of the hydrogen-form ZSM-48 molecular sieve includes the following steps: (1) Under contact conditions, silicon source, aluminum source, template agent and water are mixed evenly to obtain a gel; (2) The gel obtained in step (1) is subjected to hydrothermal crystallization treatment, and then washed, dried and calcined to obtain hydrogen-form ZSM-48 molecular sieve. The template agent in step (1) includes hexamethylammonium hydroxide and urea; preferably, the template agent also includes urease. The synthesis method controls the molecular sieve pore size and B / L acid ratio by introducing urease and using a two-stage hydrothermal treatment method, thereby realizing the control of molecular sieve properties according to the target reaction characteristics, which is beneficial to improving the yield of low-branched isoalkanes and improving the quality of lubricating oil base oil.

This invention relates to a microbial mineralization composite material for inkjet 3D printing and its preparation method. The composite material comprises powder materials and a binder. The powder materials, by weight, include: 70-85 parts magnesium phosphate cement powder, 10-25 parts fine aggregate, 0.5-3 parts a porous protective carrier loaded with urease-producing Bacillus licheniformis spores, 0.05-0.5 parts a nutrient protectant, and 1-5 parts a calcium source. The binder, by weight, includes: 94-98 parts deionized water, urea, 0.02-0.2 parts nutrients, 0.01-0.5 parts a pH adjuster, 0.5-5 parts a viscosity adjuster, and 0.01-0.2 parts a surface tension adjuster. The concentration of urea in the binder is 0.02-0.10 mol / L. This invention solves the problems of existing inkjet 3D printing materials having internal pores, microcracks, and weak interlayer interfaces after molding, limiting the improvement of the mechanical properties of printed parts in the later stages, and the difficulty in adapting the microbial mineralization system to the inkjet 3D printing process.

The present disclosure relates to a novel technique capable of highly efficiently producing a high-concentration preparation of a target substance in a production process for the target substance utilizing a microorganism under anaerobic conditions or microaerobic conditions, wherein a necessary substrate is efficiently supplied to a microbial metabolic pathway involved in the biosynthesis of the target substance and the dilution of the target substance in a reaction solution is suppressed. Specifically, the present disclosure relates to a method for producing a target substance, the method including (q) culturing or reacting cells of a microorganism having a urease activity or a treatment product of the cells in a culture medium or reaction medium (X) under anaerobic conditions or microaerobic conditions to produce the target substance, wherein, during the step (q), a urea-containing solution (Y) is added to the culture medium or reaction medium (X).

The invention provides a phthalocyanine compound as well as a preparation method and application thereof, and relates to the technical field of medicinal chemistry. The structure of the phthalocyanine compound is A-X-B. In the formula, A is a structure containing phthalocyanine and salts thereof; x is a divalent organic structure which enables A and B to be bonded through a chemical bond; and B contains a first polypeptide structure which specifically binds and / or recognizes urease. The phthalocyanine compound disclosed by the invention combines the effects of a photosensitizer and targeting, and has good targeting and antibacterial activity on helicobacter pylori.

The invention discloses a medicine for inhibiting helicobacter pylori infection, the medicine is oridonin, and the structural formula of the medicine is as shown in formula 1. In-vitro experiments show that the oridonin has a remarkable antibacterial effect on helicobacter pylori strains SS1, ATCC700392 and ICDC11101, and can remarkably reduce the urease activity of the helicobacter pylori, inhibit the formation of a biological membrane, destroy the growth of thalli and reduce the adhesion effect of the helicobacter pylori on gastric epithelial cells, and in addition, the oridonin and antibiotics are combined for use, so that the effect of preventing and treating the gastric epithelial cells can be achieved. The compound has a synergistic antibacterial effect with metronidazole and clarithromycin, has the effect of reducing the dosage of antibiotics in clinical application, and has potential application value for improving the current drug resistance situation of the antibiotics.

The application provides a self-driven BCG-nanoparticle complex and a preparation method thereof, and belongs to the fields of biological medicine and nanomaterials. The complex is a composite of BCG and cerium oxide nanoparticles with urease activity, the cerium oxide nanoparticles are coupled to the surface of the BCG in an asymmetric distribution through modified polyethyleneimine, and specifically, the surface of the BCG is connected to several modified polyethyleneimine derivatives through chemical bonds and electrostatic adsorption, and the polyethyleneimine derivatives wrap the cerium oxide nanoparticles through electrostatic adsorption. The complex can be self-driven by using the gas release and ion concentration change caused by the catalytic decomposition of urea in the environment, significantly enhances the adhesion and retention of BCG on the bladder wall, effectively overcomes the defects of low delivery efficiency and insufficient immune activation of traditional BCG therapy, simultaneously improves the tumor immune microenvironment, and can be used for preparing high-efficiency and low-toxicity bladder cancer treatment drugs.

The invention provides a method for synchronously removing suspended matters and hardness ions in water based on biomineralization, which comprises the following steps: high-hardness wastewater and a pre-mineralized urease-producing bacterium liquid are mixed and cultured, the pre-mineralization is to form a calcium carbonate shell on the surface of the urease-producing bacterium, and the urease-producing bacterium can hydrolyze urea to generate carbonate ions and ammonium ions. A mineralized shell is formed through the pre-mineralization process to protect the strain, reduce the influence of pollutants in the environment on the urease activity of the strain, increase the self weight of the strain and promote co-sedimentation of thalli and suspended solids in wastewater, hardness ions in the wastewater are converted into carbonate solid minerals through biological mineralization, removal of the hardness ions is achieved, and the removal rate of the wastewater is increased. Meanwhile, heavy metals and organic pollutants in the wastewater can be removed in the biomineralization process of the urease-producing bacteria. According to the method, synchronous removal of hardness ions and suspended solids in the wastewater is realized based on a biomineralization treatment process, and the method is environment-friendly, low in cost and easy for large-scale application.

The present invention discloses a method for fixing heavy metals based on biochar, which comprises the following steps: (1) mixing biochar with contaminated water or contaminated soil to allow the biochar to capture heavy metals; (2) mixing a bacterial solution containing urease bacteria with the biochar with heavy metals captured in step (1) to allow the biochar to capture the urease-producing bacteria in the bacterial solution; and (3) mixing a binding liquid with the biochar with urease-producing bacteria captured in step (2), allowing the mixture to react with the binding liquid, wherein the binding liquid comprises calcium chloride and urea. The present invention improves the efficiency and long-term effectiveness of the biochar fixation of heavy metals and reduces the risk of secondary pollution after the biochar fixation of heavy metals.