324 results about "Urease" patented technology

The invention relates to a coral sand soil multi-dimensional ecological low-carbon reinforcement method which comprises the following steps: S1, a urease solution is sprayed on a coral sand single-particle soil layer, a cementing liquid A is sprayed after standing, the urease solution is extracted from legume plants, and the cementing liquid A is obtained by mixing a urea solution and a calcium chloride solution; s2, on the basis of treatment in the step S1, a sporosarcina pasteurii bacterial solution is firstly sprayed, after standing, a cementing solution B is sprayed, the cementing solution B is alternately and circularly sprayed for multiple times, and the cementing solution B is obtained by mixing a urea solution and a calcium chloride solution; s3, a 3D printing geosynthetic material is laid on the basis treated in the step S2; and S4, laying a coral sand single-particle soil layer on the basis of treatment in the step S3, and repeating the steps S1 to S3. According to the method, a single particle size-foundation unit size-engineering size reinforcing system is constructed, comprehensive reinforcing from a microscopic single particle to a macrostructure can be achieved, and the reinforcing effect and efficiency are effectively improved.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention discloses a concrete repairing agent with a bacterium-enzyme synergistic mineralization effect and a preparation method of the concrete repairing agent. The preparation method comprises the following steps: culturing urealytic bacteria to obtain a spore bacterium suspension, and mixing the spore bacterium suspension with a urease extracting solution to obtain a mixed solution; mixing the soluble calcium salt solution and the urea solution to obtain a cementing solution; the concrete repairing agent with the bacterium-enzyme synergistic mineralization effect is obtained after the mixed solution and the cementing solution are mixed, and the concrete repairing agent with the bacterium-enzyme synergistic mineralization effect is applied to crack repairing of concrete to generate multiple effects of regulating and controlling crystal forms and enhancing the crack filling compactness and the adhesion effect.

The invention discloses a comprehensive prevention and control method for tobacco root knot nematode disease containing a biological agent. The method comprises the steps of crop rotation of tobacco and buckwheat / quinoa, host resistance induction of the microbial agent in the seedling raising period, tobacco planting soil quality improvement in the transplanting period and the like. The agronomic characters of tobacco seedlings are effectively improved, and the physical characters of soil are improved; the chemical properties of soil pH, alkali-hydrolyzable nitrogen, available phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium and the like are improved; the activity of soil urease, sucrase, acid phosphatase, catalase and peroxidase is improved; the content of water-soluble phenols, compound phenols and total phenols in soil is reduced; the disease index of the tobacco root knot nematode disease can be remarkably reduced, the survival rate of tobacco is increased, the application amount of chemical agents is reduced, and the healthy and sustainable development of the tobacco industry is promoted.

The invention discloses a continuous cropping Chinese cabbage planting method based on a microbial agent and application, and belongs to the technical field of crop cultivation. According to the planting method, Chinese cabbage and garlic are intercropped according to the ratio of 1: 1, and a trichoderma harzianum agent is applied. Results show that the planting method can significantly increase the growth indexes such as the plant height, the root length, the leaf chlorophyll content and the soluble sugar content of the Chinese cabbage; the soluble protein content, the peroxidase activity, the superoxide dismutase activity and the malondialdehyde content of the Chinese cabbage leaves are obviously reduced. Meanwhile, the content of rapidly available phosphorus, rapidly available potassium, effective calcium and other substances in the soil can be increased; the activities of urease, catalase and alkaline phosphatase in the soil are also obviously improved. In addition, the number of fungi, bacteria and actinomycetes in the soil is also remarkably increased. Therefore, according to the planting method, the quality of continuous cropping soil can be improved, Chinese cabbage growth is promoted, and theoretical support is provided for healthy and sustainable development of the Chinese cabbage industry.

The invention discloses a novel application of bacillus subtilis WJL-2, and relates to the technical field of microorganisms. The invention discloses a novel application of bacillus subtilis WJL-2. The preservation number of the bacillus subtilis WJL-2 is GDMCC65111, and the preservation number of the bacillus subtilis WJL-2 is GDMCC65111. The new application specifically comprises the following steps: improving soil, regulating and controlling the content of total nitrogen and organic carbon in the soil, regulating and controlling the activity of amylase / urease / cellulase in the soil, regulating and controlling the content of ammonium nitrate nitrogen in the soil, regulating and controlling the mineralization rate of the soil, regulating and controlling the nitration rate of the soil and regulating and controlling the ammoniation rate of the soil. After the strain WJL-2 is inoculated into the soil, the total nitrogen and organic carbon content of the soil is reduced, and the C / N is reduced; the activity of soil amylase is reduced, and the activity of urease and cellulase is increased; the content of ammonium nitrogen in the soil is in a trend of firstly increasing and then decreasing, and the content of nitrate nitrogen is continuously increased; the mineralization rate of soil organic nitrogen is accelerated, the ammoniation rate is in a trend of increasing first and then decreasing, and the nitration rate is in a trend of decreasing first and then increasing.

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

The invention discloses a dialysate regeneration system and dialysis equipment, the dialysate regeneration system comprises a waste liquid passage, a slow flow box, an electromagnetic valve, a reactor and an adsorption mechanism, the waste liquid passage is in through connection with the slow flow box, the slow flow box is connected with the reactor through a pipeline, and the pipeline is provided with the electromagnetic valve. The fixing amount of a urease layer is increased through the treatment film with the three cambered surfaces, the urea decomposition efficiency is improved, the diffusion path of toxins on the treatment film is shortened, meanwhile, the treatment film with the cambered surface structure can guide dialysis waste liquid entering the reactor to generate local turbulence, and the toxins are prevented from being accumulated on the surface of the treatment film; the dialyzer is arranged, the micro horse is started to drive the hollow fiber to rotate, the material exchange efficiency of blood and dialysate is improved, and the situation that protein and cells are deposited on the surface of the hollow fiber, and the service life of the hollow fiber is shortened is avoided.

The invention relates to an integrated grouting device and an offshore wind power single pile foundation anti-scour construction method based on an indigenous microorganism precuring seabed, and the method comprises the following steps: prefabricating a special integrated grouting device according to the installation condition of an offshore wind power single pile foundation; an integrated grouting device is installed on the seabed of the to-be-constructed area through a hammering method and a negative pressure method; urease microorganisms are enriched and cultivated in in-situ seabed soil through an integrated grouting device, the microorganisms are induced to generate calcium carbonate gelatinization crystals so as to bond sandy soil particles, and the overall strength of a surface seabed is improved; and a single-pile foundation is installed on the microorganism solidification seabed. Before pile sinking construction, the surface seabed of the to-be-constructed area is intensified in advance through in-situ enrichment cultivation of indigenous urease microorganisms and induction of mineralization and crystallization of the microorganisms, scouring protection of the single pile foundation in the whole service cycle is achieved, and then the local scouring problem in the pile sinking process is solved.

The invention provides an application of a lactobacillus paracasei IOB413 metagen in prevention of helicobacter pylori infection, and relates to the technical field of microorganisms. The lactobacillus paracasei IOB413 metaplasm can competitively occupy gastric epithelial cell adhesion sites to directly block the initial colonization of helicobacter pylori, so that infection preposed interception is realized, and the lactobacillus paracasei IOB413 metaplasm is suitable for early prevention of high-incidence people. The compound has dual mechanisms of site blocking and urease inhibition, and can cooperate with antibiotics to improve the eradication rate and reduce the drug resistance. Animal experiments show that the traditional Chinese medicine composition can reduce gastric mucosa proinflammatory factors in a dose-dependent manner, improve the activity of antioxidant enzymes, reduce lipid peroxidation products, relieve mucosa lesion, promote repair and reduce the risk of complications; moreover, gastrointestinal hormone disorder can be reversed, the digestive function is maintained, and related discomfort is relieved.

The invention discloses an alcaligenes faecalis UA strain, a product and application thereof, and a method for repairing an acid mine environment by acid-resistant urease-producing bacteria, and belongs to the technical fields of industrial microorganisms, microorganism application materials and environment repairing. The UA strain CGMCC No.33976 is found for the first time to produce the acid-resistant urease, the urease is stably produced in the environment with the optimum acidity of pH 4.5-pH 6.5 and the initial pH of 3.5-pH 7.5, and the enzyme production activity is the highest in the environment with the initial pH of 5.5-pH 6.5. The UA strain can effectively reduce acidity and remove metal ions in water in an acid mine wastewater real water sample, the removal rate of aluminum, manganese, zinc and cadmium exceeds 99%, and the removal rate of calcium, iron and lead is 92%-98%. The UA strain is screened from the acid mine environment, has biological safety, has industrial purposes of AMD in-situ direct treatment and acid mine environment in-situ remediation, does not need to add chemical agents to adjust the pH value of the environment, and has ecological safety and environmental sustainability.

The invention discloses high-urease microbial gel, a preparation method and a method for repairing uranium-polluted soil by using the high-urease microbial gel, and belongs to the technical field of environmental pollution microbial treatment. The preparation method comprises the following steps: 1) mixing sodium alginate, urea and a water-retaining agent to obtain a carrier solution; 2) uniformly mixing the high urease bacteria solution in the logarithmic phase with the carrier solution according to a volume ratio of 5-25% to obtain a bacteria solution mixture; and (3) dropwise adding the bacterial liquid mixture into a cross-linking agent which is stirred at a constant speed for cross-linking and curing, and freeze-drying to obtain the high-urease microbial gel. The high-urease microbial gel is applied to the periphery of a root system of a uranium-enriched plant planted in uranium-contaminated soil to be repaired, and uranium-contaminated soil is repaired through combination of microorganisms and plants. Urease-producing bacteria in the high-urease microbial gel induce decomposition of urea to generate carbonate, uranyl ions in the soil are activated, the mobility of uranium in the soil is improved, and extraction of uranium in the soil by uranium-enriched plants is promoted.

The invention provides an EICP-based sandy soil curing agent, a sandy soil curing method and application thereof, and relates to the technical field of rock and soil reinforcement. The sandy soil curing agent comprises a urease solution, a cementing fluid and a hydrophobic polymer, wherein the urease solution is prepared by salting out a urease crude extract and then carrying out solid-liquid separation; the cementing liquid comprises calcium salt, magnesium salt and urea. The curing agent is convenient to use, the solidified soil body is stable in structure, and the curing agent has excellent disintegration resistance. The invention further provides a sandy soil curing method and application of the sandy soil curing agent or the sandy soil curing method.

The invention discloses a urease-producing microorganism induced calcium carbonate deposition solution for foundation reinforcement and a preparation method and application thereof, and belongs to the technical field of foundation reinforcement. The urease-producing microorganism induced calcium carbonate deposition solution comprises a bacterial solution, a cementing solution and a sodium citrate solution, the bacterial liquid comprises bacteria capable of generating urease and citrobacter freundii. Sodium citrate in the urease-producing microorganism induced calcium carbonate deposition solution can chelate free calcium ions in advance, the concentration of the free calcium ions is reduced, the risk of precipitation at an injection port in the single-phase injection process is eliminated, and the solution can smoothly permeate into a deep layer; citrobacter freundii produces acid through metabolism and utilizes citric acid radicals through metabolism to ensure gradual release of calcium ions, so that uniform deposition of calcium carbonate is triggered. Through the synergistic effect of sodium citrate chelated calcium ions and citrobacter freundii metabolism, the solution can be uniformly distributed in the soil sample, and the problem of uniformity of single-phase perfusion is successfully solved.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

To provide a soil improvement method utilizing urea and urease, allowing reduction of ammonia generated in the course of soil improvement.SOLUTION: The present invention provides a soil improvement method in which calcium carbonate is generated from carbon dioxide produced through urea and urease to solidify soil, the method characterized by further adding a phosphorus compound and a magnesium compound to produce magnesium ammonium phosphate from at least a part of the ammonia generated through urea and urease.SELECTED DRAWING: None

The invention provides a strain of Priestia megaterium M23 and application thereof, belongs to the technical field of agricultural microbial strains, and particularly provides a strain of Priestia megaterium M23 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20232384, and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384 and a strain of Priestia megaterium M23 with the preservation number of CCTCC NO: M 20232384. The strain Pristia megaterium M23 provided by the invention has high salt tolerance and drought tolerance, has the function of promoting plant growth, improves the salt tolerance and drought tolerance of plants, can improve the soluble sugar content of the plants, increases the abundance of soil microorganisms, increases the urease activity of the soil, improves the quality of crop seeds and the like, and has important application value.

The invention discloses a grouting anchor rod suitable for microorganism induced mineralization reinforcement and an application method of the grouting anchor rod. The grouting anchor rod comprises a sleeve, an anchor head, a hollow stop-grouting plug, an arch-shaped base plate and a nut, and the anchor head is detachably installed at the anchoring end of the sleeve. Through combination of the sleeve and the hollow anchor rod, grouting channels which work independently and do not interfere with each other are formed, grouting materials and biological nutrient solutions containing urease-producing flora are injected into drill holes, pore water around anchor holes and the injected nutrient solutions are used for inducing calcium carbonate to precipitate in an anchoring area, CaCO3 crystals are deposited on an anchoring interface, and the anchoring effect is achieved. Pores are filled, the bonding strength of slurry and rock soil is improved, an anchoring layer is effectively prevented from being stripped, and the interface bonding performance is enhanced; a microorganism induced mineralization technology and an anchor rod physical reinforcement system are combined, and structure-biology-environment multi-scale collaborative reinforcement is achieved; and macrostructure stability and microcosmic particle consolidation are unified, and the enhanced, lasting, stable and eco-friendly slope supporting effect is achieved.

The invention relates to a microbial agent containing impatiens balsamina alcaligenes and arthrobacter nicotianae and a heavy metal passivation method, the microbial agent comprises a main microbial agent and an auxiliary microbial agent, and the main microbial agent comprises the impatiens balsamina alcaligenes; the auxiliary microbial agent comprises arthrobacter nicotianae; the impatiens balsamina alcaligenes is classified and named as impatiens balsamina alcaligenes, and the preservation number of the impatiens balsamina alcaligenes is CGMCC (China General Microbiological Culture Collection Center) No.14474; the arthrobacter nicotianae is classified and named as Arthrobacter nicotinovorans, and the preservation number of the arthrobacter nicotinovorans is CGMCC (China General Microbiological Culture Collection Center) No.33646. The method disclosed by the invention has a relatively good passivation effect on heavy metals, and can adjust the pH value of a water and soil environment and improve the content of urease-producing bacteria in soil.

A chitosan-heparin nanomotor and a method for producing same are disclosed. A STING agonist-encapsulated urease-based chitosan-heparin nanomotor delivers the STING agonist directly to bladder mucosal cells in the bladder, and thus can induce an immune response.

The invention relates to a heavy metal polluted soil repairing and reinforcing method by utilizing indigenous microorganisms in cooperation with CO2 carbonization, which comprises the following steps: firstly, measuring indexes of heavy metal polluted soil, stacking the heavy metal polluted soil in different areas, and collecting specific polluted soil to test microbial indexes; preparing a polluted soil-urease bacterium suspension, and culturing to form a suspension; preparing an indigenous urease bacterium solution; preparing an indigenous urease bacterium liquid-urea mixed solution; then polluted soil is pre-repaired, a curing agent and the mixed solution are mixed, and particles are prepared and cured; and finally, high-concentration CO2 is introduced for reinforced carbonization repair, and the improved soil meeting the requirements is obtained. According to the method, indigenous microorganisms and CO2 carbonization are fully utilized, the method is especially suitable for repairing and reinforcing heavy metal polluted soil containing lead, copper, zinc and the like, the problems that a traditional solidification and stabilization method is high in energy consumption and poor in economical efficiency, and an existing microorganism mineralization technology is poor in microorganism adaptability and poor in repairing effect are solved, and the method is suitable for large-scale popularization and application. The method has the advantages of simple operation process, economy, effectiveness, low carbon and environmental protection.

A ground property adjustment method characterized by: solidifying the ground by proliferating microorganisms, in the ground, that are capable of producing a biological membrane (biofilm), have urease activity, and can cause calcium ions to precipitate as calcium carbonate through decomposition of urea by the urease activity. Microorganisms may precipitate calcium carbonate on the surface of a biological membrane by incorporating carbon dioxide in the environment.

The invention discloses an ecological slope protection method based on EICP combined with mushroom dregs, which organically combines the EICP technology with the mushroom dregs, enhances the EICP reaction efficiency by utilizing the activity of organic matters and microorganisms in the mushroom dregs, and realizes the resource utilization of waste mushroom dregs at the same time. The method comprises the specific steps that the mushroom residues and EICP reaction liquid are mixed to be uniform on the clay slope surface, urea is promoted to be hydrolyzed through urease, calcium carbonate sediment is generated, and therefore the erosion resistance and stability of the slope surface soil body are enhanced. In addition, organic matters in the mushroom residues can improve the soil structure, promote the growth of plant roots, and further improve the ecological restoration effect of the slope. Compared with the prior art, the method has the advantages of being low in cost, environmentally friendly, easy and convenient to construct and the like, and is suitable for various slope ecological protection projects.

The invention discloses a high-sensitivity visual detection kit for the activity of human structure-specific nuclease FEN1 and application of the high-sensitivity visual detection kit. A turnup flap structure in FDP is specifically cut by FEN1 to generate a dumbbell-shaped DNA probe with a notch, the notch of the probe is connected under enzyme catalysis to generate a closed annular dumbbell-shaped DNA probe, rolling circle amplification is carried out in the presence of an amplification primer and enzyme, a urease modified signal probe is added into an amplification product, and the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows. An amplification product and a signal probe complement are separated by virtue of magnetic microspheres and are added into a urease substrate solution, urea in the solution is efficiently catalyzed by urease in the complement to cause change of a pH value, so that the color of a phenol red indicator is changed, and the FEN1 activity is detected. The kit is high in sensitivity, simple and convenient to operate, free of additional instruments and equipment, low in cost and large in application potential, results can be observed by naked eyes, and the kit is used for screening FEN1 inhibitor candidate drugs, and the drug discovery efficiency is remarkably improved.

The invention relates to the field of ocean pile foundation maintenance, and discloses a construction method for reinforcing a soil body around a pile based on microbial capsules and a reinforcing structure. The construction method comprises the following steps: culturing urease-producing bacteria; the method comprises the following steps: mixing a first water-soluble capsule for encapsulating cementing powder, a second water-soluble capsule for encapsulating bacterial sludge and graded broken stone and grit to form a mixture; and the mixture is placed in an expected reinforcement area around the pile. Bacterial sludge and cementation powder are packaged through two kinds of capsules respectively and mixed with graded broken stone, then the mixture is put around a pile, the capsules are supplemented regularly, and microorganism-induced mineralization reinforcement is achieved. According to the reinforcement method combining the microbial capsules with the gravel, the anti-scouring capacity of the soil around the pile can be effectively enhanced through the calcium carbonate deposition reaction generated by microorganisms, and compared with a traditional physical protection and chemical reinforcement method, better environmental adaptability and sustainability are achieved; and salt erosion and pollution of a traditional chemical grouting material are avoided.

The invention discloses a method for preparing fermented soybean meal through synergistic fermentation of saccharomyces cerevisiae FNFHSc18 and bacillus subtilis FNFHBS08 and application of the fermented soybean meal, combined fermentation of the saccharomyces cerevisiae FNFHSc18 and the bacillus subtilis FNFHBS08 is utilized, the method has the advantages of being short in fermentation period, high in efficiency and low in cost, the prepared fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is increased. The contents of crude protein and soluble protein are obviously improved, anti-nutritional factors such as antigen protein, oligosaccharide and urease are greatly reduced, and the nutritional value of the soybean meal is comprehensively improved; the invention also provides an application of the fermented soybean meal feed in breeding, and the fermented soybean meal is used for replacing 30% of fish meal protein, so that the final weight, the weight gain rate and the specific growth rate of the largemouth bass can be obviously improved, and the growth performance of bred animals is enhanced.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

According to the method for improving solidification of the aeolian sandy soil by constructing the synthetic community based on the composite urease bacteria, the composite urease bacteria are screened based on collection and treatment of local aeolian sandy soil and strains to construct the synthetic community, the composite urease bacteria liquid is sprayed on the aeolian sandy soil, and the composite flora can improve the deposition rate and quality of calcium carbonate through a synergistic effect, so that the solidification of the aeolian sandy soil is improved. Meanwhile, the adaptability to environment change is enhanced, so that the aeolian sandy soil is effectively solidified; calcium carbonate deposition and solidification of aeolian sandy soil are carried out through a composite urease bacterial solution, and the method comprises the following steps: step 1, sample collection and pretreatment; step 2, primarily screening and separating urease-producing strains; 3, performing urease activity quantitative detection and secondary screening; 4, testing the extreme environment adaptability; step 5, preparing a composite urease bacterium community; and step 6, preparing a composite urease bacterial liquid.

The invention discloses a method for enhancing the biological nitrogen fixation capacity of soil based on micro-plastics, which comprises the following steps: adding poly (adipic acid-butylene terephthalate) micro-plastics into target soil, and the addition amount is 0.2-2% of the mass of the soil. The activity of dehydrogenase and urease of rhizosphere microorganisms is activated through the degradation process of the poly (butylene adipate-co-terephthalate) micro-plastic in soil, and microorganism groups with a nitrogen fixation function are specifically enriched, so that the expression of nitrogen fixation genes is up-regulated, the biological nitrogen fixation capacity of the soil is enhanced, and the nitrogen condition of the soil is improved.