660 results about "Urease" patented technology

A peritoneal-based (“bloodless”) artificial kidney processes peritoneal fluid without need for additional fluids (“waterless”). Fluid is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s) (“dialysate-pheresis”). It is then reconstituted with additives and returned into the peritoneal cavity, thereby reducing protein-loss and providing oncotic-pressure for ultrafiltration. The protein-free stream is used to produce free water, and an alkaline or acid fluid for optimization of the composition of the regenerated stream. The unused protein-free stream can be used to “reverse flush” the separator to maintain its patency and the excess discarded for fluid-balance regulation. Compared to prior art, immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

Methods and devices for providing dialysis treatment are provided. The device includes a resin bed including zirconium phosphate, zirconium oxide, and urease.

Apparatuses, systems, and methods for the performance of kidney replacement therapy having or using a dialyzer, control components, sorbent cartridge, and fluid reservoirs configured to be of a weight and size suitable to be worn or carried by an individual requiring treatment are disclosed. The system has a controlled compliance dialysis circuit, where a control pump controls the bi-directional movement of fluid across a dialysis membrane. A first sorbent cartridge is provided for use in a portable treatment module having activated carbon and zirconium oxide. The system also provides for the monitoring of an inlet and outlet conductivity of a sorbent cartridge containing urease to provide a facility to quantify or monitor the removal of urea by a detachable urea removal module.

A peritoneal-based (“bloodless”) artificial kidney processes peritoneal fluid without need for additional fluids (“waterless”). Fluid is separated into a protein-rich stream and a protein-free stream. The protein-rich stream is regenerated using a sorbent assembly, and its protein composition can be modified by removal of selected protein(s) (“dialysate-pheresis”). It is then reconstituted with additives and returned into the peritoneal cavity, thereby reducing protein-loss and providing oncotic-pressure for ultrafiltration. The protein-free stream is used to produce free water, and an alkaline or acid fluid for optimization of the composition of the regenerated stream. The unused protein-free stream can be used to “reverse flush” the separator to maintain its patency and the excess discarded for fluid-balance regulation. Compared to prior art, immobilization of urease allows more protein rich fluid to be regenerated and re-circulated into the peritoneal cavity for toxin removal and allows practicable development of portable and wearable artificial kidneys.

The present invention is directed to degassing devices for dialysate circuits. One embodiment has a first housing and a second housing positioned within the first housing in an annular relationship. A second embodiment comprises a dialysate regeneration system with urease, a dialyzer, and a housing with an external wall, where the external wall is exposed to atmosphere and comprises a material that passes gas but does not pass liquid and where the housing is positioned between the urease and dialyzer.

The invention discloses a microbiogenic cement and concrete and a production method thereof, which mainly mixes the Bacillus pasteurii solution for producing urease and an admixture of containing urea and mineral calcium salt and obtains the microbiogenic cement after reacting in a certain condition; adds admixture such as sand etc. into the mixture of mixing the bacterial solution and the mixture and reacts to generate the microbiogenic concrete. The microbiogenic cement or concrete of the invention can be used on protection and restoration of masonry ancient architectures and repair of cracks on concrete building or treatment of liquescent sandy soil. The invention provides a permanent method of safety, effectiveness and without environmental hazard for repair and strengthening technology of large quantity of civil architecture and structure; the invention has the advantages of the good effects, the good weatherability, the good permeability of gas and the good permeability of water and being especially suitable for treatment of the sandy soil; the invention is safe and environmental-friendly, and belongs to the environmental-friendly restoring material.

A breath test for diagnosing the presence of Helicobacter pylori in a subject is described. The method of diagnosing Helicobacter pylori is performed as follows. First, a safe and effective amount of urea, preferably appropriately labelled, is administered to the subject. Second, a plurality of the exhaled breaths of the subject is analyzed to detect the concentration of a cleavage product or products, produced when urease cleaves the substrate. The measured concentrations are then fitted to a curve, and the derivative is then calculated, to indicate the presence or absence of Helicobacter pylori infection in the subject.

The invention discloses crack self-remediation regenerated concrete based on urease production microorganism mineralization deposition and a preparation method. The crack self-remediation regenerated concrete comprises components, namely, expanded perlite carried with urease production microorganisms, cement, stone, sand, silica fume, water, urea, calcium chloride, a urease production microorganism suspension and a water reducing agent. The urease production microorganisms are adopted as a concrete crack remediation agent, urease can be generated through metabolism through the urease production microorganisms, and the urea can be decomposed into NH4<+> and CO3<2+>, and furthermore the calcium carbonate can be mineralized and deposited to remedy cracks. In the preparation process, a part of the crack remediation agent is directly mixed with the concrete, then cracks and holes in regenerated crude aggregate self and weak adhesion areas between regenerated aggregate and a new cement stone base can be remedied, and thus the mechanical property of the regenerated concrete can be improved; meanwhile, the other part of the crack remediation agent is firstly adsorbed into an expanded perlite carrier and is further mixed into the concrete, then the crack self-remediation property of the regenerated concrete in the service period can be improved, and thus the anti-penetrability performance and the durability of the regenerated concrete can be improved.

Methods and devices for providing dialysis treatment are provided. The device includes a resin bed including zirconium phosphate, zirconium oxide, and urease.

The invention relates to an organic-inorganic composite fertilizer, which consists of straws, excrements and fertilizers. By weight, in the composite fertilizer, the ratio of N, P2O5 and K2O is 10 to 35:8 to 12:5 to 8, and the ratio of C and N is 10:15. The organic-inorganic composite fertilizer has very obvious effects for improving biomass of wheat and increasing readily available nutrient of soil, urease activity, microbial biomass in the carbon-nitrogen aspect.

The invention relates to algae-enhanced urea and a production method thereof. According to the invention, a fertilizer is produced through steps that: natural algae and urea are adopted as raw materials; algae is subject to microbe fermentation, and is subject to processes such as extraction and press-filtration, such that an algae enhancing liquid is prepared; molten urea solution and the algae enhancing liquid are granulated in an urea granulating tower, such that the algae-enhanced urea provided by the invention is obtained. With the algae-enhanced urea, soil urease activity can be substantially inhibited. The algae-enhanced urea has an enhanced fertilizer releasing-retarding effect. With the algae-enhanced urea, a utilization rate of fertilizer is improved by 5-10%, and agricultural cultivation can be simplified; nutrient loss can be reduced; secondary pollution can be avoided; and agricultural production cost can be reduced. The algae-enhanced urea is advantaged in wide raw material source, convenient transportation, and convenient application. With the algae-enhanced urea, mechanical working can easily be carried out. Therefore, the algae-enhanced urea has a wide prospect in production.

A disposable urea sensor has a laminated body having a fluid sample inlet end and an electrical contact end, a fluid sample inlet, a substantially flat sample chamber in communication between the fluid sample inlet and a vent opening, the sample chamber being adapted to collect a fluid sample through the fluid sample inlet, a working electrode and a reference electrode within the sample chamber, and a reagent matrix disposed on the working electrode wherein the reagent matrix contains urease.

The invention relates to a modified glutamic acid fertilizer synergist and a production method and an application thereof. The fertilizer synergist is a modified glutamic acid fertilizer synergist obtained by extracting glutamic acid with small molecular amount from weathered coal and humate serving as raw materials with diluted alkali or diluted acid and hydrogen peroxide and adding chelated state trace elements. Due to the adoption of the modified glutamic acid fertilizer synergist, the soil urease activity can be suppressed remarkably, the ammonia volatilization loss is reduced, and slow release of a fertilizer is enhanced; root growth of crops can be facilitated, root activity is increased, and the capabilities of crops of absorbing phosphorus, potassium and medium and trace elements are increased; and nutrient loss can be reduced, secondary pollution is avoided, and the production cost of a synergic fertilizer is reduced.

The invention relates to humic acid urea and a preparing method thereof. The preparing method includes: firstly preparing humic acid synergistic liquid, then adding the humic acid synergistic liquid into urea melt liquid during urea producing, and finally performing high tower cooling granulation on mixture of the humic acid synergistic liquid and the urea melt liquid to obtain the humic acid urea. The humic acid urea has the advantages of being high in nitrogen content and productivity, low in cost, good in effect, suitable for field crops, easy to popularize and use and the like. In production practice, the humic acid urea reduces ammonia volatilization loss by restraining soil urease activity, has the characteristics of being excellent in slow release performance, high in in-season nutrient use ratio, environment-friendly and the like, is simple in manufacture technology, wide in raw material sources, easy to operate, low in cost, high in productivity and environment-friendly, and has wide application prospect in specific production practice.

PCT No. PCT / SE95 / 01212 Sec. 371 Date May 29, 1997 Sec. 102(e) Date May 29, 1997 PCT Filed Oct. 17, 1995 PCT Pub. No. WO96 / 14091 PCT Pub. Date May 17, 1996A preparation for administering isotope-labelled urea together with acid in a solid form that is influenced by activity of Helicobacter pylori in the stomach only, avoiding any non-Helicobacter (unspecific) urease activity in the oral cavity and the pharynx. The preparation dissolves very quickly in the stomach. The preparation is used to show increased urease activity in the stomach in association with an ongoing Helicobacter pylori infection.

A method of forming a high strength cement in a permeable starting material, the method comprising the step of combining the starting material with effective amounts of (i) a urease producing micro-organism; (ii) urea; and (iii) calcium ions and wherein the effective amount of the urease producing organism provides a urea hydrolysis rate, under standard conditions, of 0.5-50 mM urea hydrolysed.min-1.

The present invention provides an improved method of producing a stable acid denatured soy / urea adhesive having improved wet and dry strengths, with more efficient production and lower production costs. The method comprises combining urea with soy flour that has been acid treated until denatured and substantially free from urease activity. The soy flour is preferably lowered to a pH of 2.0 to 4.0 for at least 1 minute. Optionally, the method may also include adding a crosslinking agent, diluent or both to the soy flour / urea adhesive and / or adding an emulsified or dispersed polymer. Adhesives and dispersions prepared according to the methods of this invention offer increased stability and strength properties.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis. The sorbent cartridge is configured to allow an amount of urease to be added to the sorbent cartridge. A urease solution can be injected into the sorbent cartridge to replenish the urease containing module, or solid urease can be added to the sorbent cartridge. The sorbent module can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

A sorbent dialysis cartridge is provided for removal of uremic toxins from dialysate wherein the sorbent cartridges can use non-enzymatic urea-binding materials in place of urease. The cartridge can have a first sorbent layer loaded with a polymerizable urea complexing agent and a second sorbent layer loaded with a crosslinker. The crosslinker can be crosslinkable with a soluble urea complex reaction product of the polymerizable urea complexing agent and urea when passing through the first sorbent layer to form a crosslinked polymeric urea complex which is attachable to the second sorbent layer. In another option, a sorbent layer can be used which has an insolubilized crosslinked polymeric urea-bindable complex attached thereto, wherein the crosslinked polymeric urea-bindable complex can be a reaction product of a crosslinker and polymerizable urea complexing agent. Methods and sorbent dialysis systems using the cartridge, and methods of making the sorbent material, are provided.

The invention provides a composite microbial flora and an enzyme product thereof, and a method of applying the composite microbial flora and the enzyme product thereof in soil restoration. The composite microbial flora is composed of a microbial flora and a bioactive enzyme, wherein the microbial flora is composed of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, pediococcus pentosaceus, pichia farinose and pediococcus acidilactici, and the bioactive enzyme is composed of phytase, protein hydrolase, cellulose hydrolase and urease. Compared with the prior art, an effective microbial community and a microecological environment are constructed to realize biological catalysis and improve the content of organic matters and beneficial microbial flora in soil, and thus, the fertility of the soil is improved; biochemical degradation rate of soil pollutant can be substantially accelerated, digestion and biodegradation of the pollutant can be accelerated, and final inertial products like carbon dioxide, water and nitrogen are obtained; and toxic intermediate produced in the process of biochemical degradation can be rapidly removed, odor is eliminated, and emission of organic volatile matters (VOC) is reduced.

A method for producing nanoscale titanium dioxide particles which are coated with an oxide, a hydroxide or an oxide hydroxide of Al, Ce, Zr and / or Si wherein the enzymatic precipitant system urease / urea is used. Said coated titanium dioxide particles can have an average size of less than 50 nm, the coating providing effective protection against photocatalytic reactions. The coated particles are suitable, in particular, as UV absorbers in a transparent organic matrix.

The invention discloses an organic-inorganic mixed fertilizer containing alginic acid and a production method thereof. The organic-inorganic mixed fertilizer containing alginic acid comprises the following raw materials in parts by weight: 45-50 parts of carbamide, 18-20 parts of ammonium phosphate, 16-18 parts of potassium sulphate, 6-8 parts of seaweed fermentation residues, 8-10 parts of a seaweed synergist, 6-8 parts of a humic acid synergist, 3-5 parts of an EDTA chelated-state microelement fertilizer, 5-7 parts of plant ash, 3-5 parts of modified magnesia and 0.2-0.3 part of gibberellins. High-quality and high-effect synergist products are added into the technical field of value addition of the fertilizers, so that the high urease inhibition effect is achieved; the conversion from ammonium nitrogen to nitrate nitrogen is controlled; the utilization rate of the fertilizer is increased; the root growth of the crops is facilitated; the content of nutrient in the fertilizer is almost not influenced; the content of nitrogen in the value-added carbamide is not smaller than 30%; the organic-inorganic mixed fertilizer is obvious in synergistic effect and safe and effective in use; organic components are used for increasing the utilization rate of the fertilizer; inorganic components are used for improving the water-retaining property of the fertilizer; the action time of the fertilizer effect is prolonged; the absorption of nutrients in the crops is facilitated; the quality of agricultural products is improved.

The invention discloses a preparation for inhibiting soil nitrogen loss as well as a preparation method and an application thereof. The preparation is prepared from the following raw materials in percentage by mass: 1-40% of 2-chloro-6-trichloromethylpyridine, 0-5% of hydroquinone, 5-20% of accessory ingredient and 35-90% of solvent. The preparation disclosed by the invention can be evenly dispersed in an aqueous phase, and the preparation and a nitrogenous fertilizer are evenly applied into soil so as to effectively alleviate speed of urea to be hydrolyzed into ammonia under the urease action and also effectively inhibit the process of oxidizing NH<4+> into NO<3->; NO<2-> and NO<3-> leaching loss is reduced, and NOX and N2 can be further prevented from escaping because of denitrification; and the utilization rate of the nitrogen fertilizer is improved so as to improve the quality and the yield of the crops and lighten pollution to environment by the nitrogen fertilizer.

The invention relates to a humic acid synergia compound fertilizer and a preparation method thereof. The method comprises the steps of: preparing a humic acid synergia solution, preparing a granulating material, coating an enveloping layer and drying, screening, cooling and the like. A humic acid synergist disclosed by the invention has the characteristics of being trace and efficient, low in addition volume, free from influence on nutrient content of the fertilizer, and favorable for regulation on the formula, and has the advantages of being high in output, low in cost, good in effect and the like. With the coated humic acid synergia compound fertilizer, the nitrogen utilization efficiency can be improved by 5-10 percentage points by inhibiting the soil urease activity; phosphorus, potassium and medium trace elements in soil can be activated; the absorption of crops is enhanced; and the humic acid synergia compound fertilizer has the characteristics of being excellent in slow release performance, high in utilization rate of butrients in season, and environment-friendly, and is wide in application prospect in specific production practice.

The invention relates to coated carbamide, in particular to a totally biodegradable coated carbamide and a preparation method thereof. The coated carbamide consists of a coated layer and a carbamide core. The main body of the coated layer is polylactic acid and / or polybutylene succinate. The coating process adopts fluidized bed spraying-coated technology, and comprises the following steps: dissolving polylactic acid and / or polybutylene succinate in trichloromethane, adding carboxymethylcellulose, organic and / or inorganic conditioner, urease and / or nitrification inhibitor in the solution, spraying and coating the surfaces of the fluidized carbamide after uniformly mixing the mixture under the stirring of a stirrer to form the uniform and complete organic polymeric membrane layer. The process has the advantages of easy implementation, and capacity of effectively slowing down the release of the nutrient to the outside and the conversion rate of nitrogen in soils, effectively controlling the release of the nitrogen nutrient in soils, and reducing the pressure on environment caused by the rapid release of nutrient. The coated material can be totally biodegradable, and the degradation products have no secondary pollution to the environment.

A process for producing an antibody enzyme which involves an antibody structure analysis step of confirming the presence of a catalyst triplet residue structure wherein a serine residue, an aspartate residue and a histidine residue or a glutamate residue are located stereostructurally close to each other in the stereostructure of an antibldy anticipated based on its amino acid sequence. Since the above-described catalyst triplet residue structure is a structure specific to an antibody enzyme, an antibody enzyme can be efficiently screened by using the same. Examples of the antibody enzyme as described above include an antibody enzyme against Helicobacter pylori urease and an antibody enzyme against chemokine receptor CCR-5.

The invention relates to a microorganism slab grouting device and a microorganism slab grouting method used for reinforcing a liquefiable foundation. The device comprises a grouting panel, a pipeline switching device, an extended panel and grouting pipes. The grouting method comprises the following steps: a. vertically ramming microorganism slab grouting devices arranged in I and II forms into a to-be-reinforced stratum; b. carrying out butt joint on one ends of the first and second grouting pipes with first and second slurry passages in the microorganism slab grouting devices, connecting the other end of the first grouting pipe with a valve, a grouting pump and urease-producing microorganism bacterial liquid storage pond in sequence, and connecting the other end of the second grouting pipe with the valve, the grouting pump and a cementing solution storage pond in sequence; and c. opening the valve and the slurry injection pump to grout for the stratum, wherein the microorganism slab grouting devices are lifted uniformly during grouting, and after grouting is completed, all microorganism slab grouting devices in the field are pulled out and stand for 12h, and finally, underground water is pumped out for sewage treatment. Compared with the prior art, the microorganism slab grouting device and method can simplify the procedures, shorten the duration and eliminate the problem of size effect.

The present invention provides one kind of genetic engineering multivalent subunit vaccine for preventing and treating human helicobacter pylori infection and its preparation process. The vaccine consists of active helicobacter pylori UreB fragment UreB414 as the central antigen component, other combined protective antigens, and intramolecular or extramolecular adjuvant. Compared with univalent vaccine, the multivalent combined vaccine can stimulate the body to generate more powerful and more comprehensive helicobacter pylori resisting specific immune reaction.

The present invention provides a kind of efficient enzyme combining stabilizer with high stabilizing effect on enzymes for clinical diagnosis reagent. The enzyme combining stabilizer consists of bovine serum albumin, EGTA, 1, 2-dithio threitol, potassium gluconate, sodium chloride, proclin300, magnesium acetate, etc. It has powerful stabilizing effect on lactate dehydrogenase, sarcosie oxidase, urease, creatinine enzyme, creatine hydrolase, etc.