110results about How to "Fewer extraction steps" patented technology

The invention relates to a method for constructing a biosensor, specifically to a method for constructing a nanocellulose paper-based biosensor. The objective of the invention to overcome the problemsof low detection efficiency and poor detection accuracy of conventional paper-based biosensors caused by great internal porosity and rough surfaces in the prior art. The method comprises the following steps: 1, peparation of nanocellulose: sequentially subjecting a cellulose raw material to extraction and delignification so as to obtain total cellulose, and then carrying out mechanical pretreatment, chemical-mechanical mixed pretreatment or biomechanical mixed pretreatment to obtain aqueous hemicellulose-containing nanocellulose liquid; 2, preparation of nanopaper; and 3, construction of thenanopaper-based biosensor. Detection results show that the nanopaper prepared in the invention has no obvious voids and micron-order roughness, wherein the porosity of the nanopaper is more than 1%, and the roughness of the nanopaper is less than 1 [mu]m. The method provided by the invention is applied to the field of biosensors.

The invention relates to a method for modifying waterborne wood coating, in particular to a method for chemically modifying the waterborne wood coating by using nano-cellulose dispersed graphene, aiming at solving the problem that the existing waterborne wood coating modified by using nanometer materials is poor in nano particle dispersity, so that the waterborne paint film is poor in mechanical properties. The method comprises the following steps: 1, preparing a hemicellulose-containing nanocellulose aqueous solution enabling cellulose raw materials to be sequentially subjected to extractiontreatment and delignification treatment to obtain holocellulose; then, carrying out mechanical pretreatment or chemical and mechanical mixed pretreatment to obtain a hemicellulose-containing nanocellulose aqueous solution; 2, stably dispersing graphene by using nano-cellulose; 3, carrying out in-situ chemical modification on the waterborne wood coating by using the nano-cellulose dispersed graphene. The method can remarkably improve the mechanical properties, such as adhesion, wear resistance, hardness and impact resistance, of the waterborne wood coating, and even endows the paint film with certain functionality such as electrical conductivity, thermal conductivity, ultraviolet resistance and aging resistance. The method provided by the invention is used for the field of waterborne wood coatings.

The invention relates to a preparation method of a high concentration ultrahigh molecular weight polyethylene fiber spinning solution. The preparation method comprises the following steps: step one, subjecting polyethylene powder, a mixed solvent, and an antioxidant to pressurization, heating, stirring, and swelling to obtain suspension of swelled body; step two, subjecting the suspension obtained in the step one to reduced pressure flash evaporation, separating and storing a solvent A with a low boiling point in a buffer tank, and discharging the suspension after flash evaporation; and step three, feeding the suspension obtained in the step two into a double screw extruder to carry out high temperature dissolution to radically unwind molecular chains through mixing by double screws so as to obtain the high concentration polyethylene fiber spinning solution. Compared with the prior art, after the swelling process, the swelled suspension is rapidly cooled through flash evaporation so as to prevent excess swelling. The preparation method can prepare a uniform polyethylene spinning solution with a polyethylene mass percentage of 15 to 50%. The solvent extraction step is not needed, the production efficiency is largely improved, at the same time, the low boiling point solvent generated in flash evaporation can be condensed, recovered and reused, and the production cost is reduced greatly.

The invention relates to a detection method and a kit for thrombin based on double Aptamer sandwich structure open ring mediated isothermal amplification. The detection method comprises the followingsteps: mixing a double Aptamer sandwich structure with a LAMP (Loop-Mediated Isothermal Amplification) primary base structure; forming an LAMP base structure under the action of Nb.BsrDI incision enzyme; carrying out LAMP under the action of primers; adding heme in an amplified product; detecting the thrombin by using an ABTS-H2O2 reaction, wherein the 3' terminal of the double Aptamer sandwich structure is provided with a free single-chain sequence, and initiating the LAMP. The invention also provides a kit for the method. The detection method disclosed by the invention first combines a double Aptamer sandwich detection method, an LAMP technology and a G-quadruplex-heme DNA enzyme enzymatic reaction and is a novel detection method with high sensitivity and high specificity for the thrombin.

Provided is an electrochemical synthetic method of 1,5-diaminonaphthalene, which includes four steps that firstly the electrochemical synthetic of diaminonaphthalene is performed in an electrolytic cell which contains a cathode chamber and an anode chamber which are separated by a cation-exchange membrane which is taken as a permeable membrane, copper sheets and a calomel electrode which are installed in the cathode chamber are respectively taken as working electrodes and a saturated reference electrode, and ruthenium-plated iron screens which are arranged inside the cathode chamber are taken as auxiliary electrodes. Secondly catholyte is mixed solution of solvent N, N-dimethyl formamide, sulfuric acid solution supporting electrolyte and substrate 1, 5-diaminonaphthalene, and anolyte is sulfuric acid solution of which the volume is equal to the volume of the catholyte. Thirdly electrolyzation is performed at room temperature and under the condition that the working electrodes are driven by a certain constant potential relative to the saturated reference electrode. Fourthly after the electrolyzation is finished, the electrolytic solution is post-processed to obtain the product of 1, 5-diaminonaphthalene. The method has the advantages of simple requirement, easy preparation of the electrodes, ready availability of raw material, greening synthetic process, normal reaction temperature, small energy consumption, high yield and suitability to industrial production.

The invention discloses a method for fast and accurate quantitative determination of circulating DNA in blood. The method comprises the following specific steps that processing of plasma or serum is performed: peripheral venous blood is taken and arranged in an EDTA anticoagulant tube for standing at room temperature, or self-clotting whole blood is taken to undergo centrifugation under 1800-2200 g of centrifugal force for 4 to 6 minutes, upper-layer plasma is taken, the same volume of diluted reagent is added, even mixing is performed, stirring is performed at the temperature of 92-97 DEG C for 6 to 10 minutes, then centrifugation is performed again under 15000-17000 g of centrifugal force for 8 to 12 minutes, and supernate is taken as a template for PCR; a quantitative PCR reaction system is configured; quantitative PCR reaction is performed. The quantitative PCR reaction can be performed by directly using the processed plasma or serum, PCR reaction is not influenced, the amplification efficiency is consistent with the amplification efficiency of purified DNA, DNA extraction steps and experimental errors are decreased, a new fluorescent dye SuperGreen which is higher in concentration, high in fluorescent signal value and response sensitivity and capable of accurately detecting plasma free DNA as low as the nanogram level can be adopted, the reagent price is low, the using cost of a DNA extraction kit is reduced, and operation is easy and convenient.

The invention discloses a method for quantitatively determining blood circulation DNA (Deoxyribonucleic Acid). The method comprises the following steps of: obtaining 1ml of peripheral venous blood of the same tumor patient and placing the peripheral venous blood into an EDTA (Ethylene Diamine Tetraacetic Acid) anti-freezing pipe; standing for 4 hours at 10-20 DEG C; centrifuging 2000g of peripheral venous blood for 5 minutes; taking 100-200 microliters of upper-layer blood plasma; adding a buffering solution with the same volume and sufficiently and uniformly mixing; standing at 95 DEG C for 5-10 minutes; centrifuging 16000g of peripheral venous blood for 10 minutes; and taking a liquid supernatant as a template to be directly used for quantitative PCR (Polymerase Chain Reaction) amplification. The method disclosed by the invention has the beneficial effects that 1. blood plasma or blood serum can be directly used for a quantitative PCR reaction through special technical process treatment and the PCR reaction is not influenced; ratios of amplification efficiencies to the purified DNA are consistent, and extraction steps and experimental errors of the DNA are reduced; and 2. an adopted novel fluorescent dye Super Green has slight inhibition effect in a PCR amplification process and high concentration can be used; and a fluorescence signal value is high and the reaction sensitivity is high, and blood plasma free DNA which is at a nanogram level can be accurately detected.

The invention discloses a ground object target-oriented Gaofen-6 remote sensing satellite data validity detection method, relates to the technical field of satellite remote sensing, and aims to detectthe coverage rate of various interference factors in remote sensing satellite data so as to evaluate the validity of the remote sensing data. A partitioning method is adopted for the remote sensing image, and then detection is conducted based on image blocks; Gist image features are extracted from the image blocks, and then classification is performed on an SVM classifier based on the GIST imagefeatures; for classification results obtained by SVM classification, different detection processes are selected for detection according to different conditions; according to the method, the coverage rate of interference factors such as cloud, shadow and snow can be effectively detected for the Gaofen-6 remote sensing satellite data of any size, the effectiveness of the image data is rapidly and accurately evaluated, typical ground object target extraction is carried out on the image meeting the requirement, and the target extraction efficiency is effectively improved.

The invention relates to a multifunctional solid state fermentation reactor. The reactor comprises a main tank, an auxiliary system and a control center, wherein a tank cover is fixed at the upper port of the main tank through a flange; a cylinder port is fixed on the top of the tank cover; the upper end face of the cylinder port is provided with four ports; pipelines connected with the four ports are connected with a gas purification module, a pressure meter and a gas analysis module through valves; the tail end of the pipeline of a first port is connected with a four-way pipe; and one port of the four-way pipe is connected with the main tank through a gas pipeline, and the formed gas forms a loop from a fourth port to a fifth port; and the auxiliary system consists of an aseptic inoculation part, a circulating gas part and a foreign gas part. The reactor can realize a closed solid state fermentation process without mixed bacterial pollution or pressure pulsation, can be applied to the process of quickly decomposing solid organic matters, and can monitor various parameters in the fermentation process, and a method for extracting a required fermentation product in the reactor in situ is provided.

The invention discloses a method for extracting and purifying resveratrol from polygonum cuspidatum, ethanol, iron salt and alkali are used as extraction media in the method, a multi-stage dynamic countercurrent extraction technology is adopted, and the steps of desalination, purification, acidification, enzymatic hydrolysis, concentration, impurity removal and the like are sequentially carried out extraction liquid, thereby preparing a resveratrol product. The method has the advantages of simple process, short operation time, low production cost, high extraction efficiency, no organic solvent and easy industrial production.

The invention discloses a method for rapidly detecting virus of a potato tuber. The method is characterized by comprising the following steps of: extracting the RNA of the potato tuber, performing reverse transcription-polymerase chain reaction (RT-PCR), detecting PCR products through a nucleic acid colloidal gold chromatography test strip, and the like. The method suitable for rapidly detecting the virus of the potato tuber is established by combining the RT-PCR technology with the nucleic acid colloidal gold chromatography technology on the premise of optimizing a method for extracting the RNA of the virus of the potato tuber. The method has the greatest characteristic that: an RNA extracting solution is developed, the RT-PCR detection can be directly performed after a potato sample to be detected is ground, an RNA extraction step in the conventional method is eliminated, and the problem that the RNA is difficult to extract due to high starch content of the potato tuber is solved; and moreover, the developed nucleic acid colloidal gold chromatography test strip can finish the detection of the PCR products within ten minutes, electrophoresis and the dyeing of ethidium bromide are not required, and the method is rapid, practical, safe and pollution-free.

The invention discloses a method for synthesizing (R)-2-hydroxy acid by a biological enzyme method. The method comprises the following steps of taking a wet cell mass obtained by fermenting a recombinant genetic engineering strain as a catalyst, racemization 2-hydroxy acid shown in a formula (I) as a substrate, glucose as a cosubstrate, and a buffer solution of which the pH value is 6.0 to 9.0 as a reaction medium to form a reaction system; performing a conversion reaction under the conditions that the temperature is 20 to 50DEG C and the rotating speed is 150rpm; after the reaction is complete, separating and purifying a reaction solution to obtain the (R)-2-hydroxy acid, wherein the recombinant genetic engineering strain is recombinant escherichia coli containing 2-hydroxy acid dehydrogenase (HADH) genes, carbonyl reductase KAR genes and glucose dehydrogenase (GDH) genes. According to a single-bacteria, double-plasmid and trienzyme co-expression system disclosed by the invention, the conversion between the racemization 2-hydroxy acid and single-configuration (R)-2-hydroxy acid through an engineering strain is innovatively and successfully realized, the culture of multiple bacteria is avoided, the total concentration of bacteria is reduced, the reaction process is simplified, and extraction steps of an intermediate product are reduced; in addition, the catalytic efficiency is also remarkably improved; the system is more suitable for industrial application of a cascade catalysis system.

The invention provides a process for preparing extract of haw flavones for injection, which is characterized by simplified procedure, low chemical residual, light contamination and low cost. The extracted haw flavones can achieve a content meeting national standard for injections.

The invention discloses a synthetic method of bis-(2-dimethylaminoethyl)ether. The method comprises the following steps: (1) chlorination is carried out by using 2-dimethylethanolamine as raw material and thionyl chloride as a chlorinating agent to obtain chloride hydrochloride; (2) 2-dimethylethanolamine and sodium are used as the raw material and reacted to obtain a sodium alkoxide solution; (3) etherification: chloride hydrochloride is added into the sodium alkoxide solution, then a catalyst is added for etherification to obtain a reaction solution; the reaction solution is filtered to obtain filtrate; (4) separation and purification: vacuum rectification of the filtrate is carried out to obtain bis-(2-dimethylaminoethyl)ether.

The invention discloses a novel method for extracting recalcitrant plant tissue DNA. In this method, an extract buffer for use is sodium laurate extract buffer. The method has the advantages that an anionic surfactant good in biological degradability, sodium laurate, is used as an extract buffer, secondary substances such as polysaccharides, pigments and polyphenols present in pear tissues can be eluted, the method does not require water bath and thereby is time efficient and simple to perform, degradation of DNA in an extraction process is maximally reduced, DNA harvest rate is high, harvested DNA is complete, and the defect that the quantity of DNA extracted with a kit is low, DNA extraction by CTAB (cetyl trimethyl ammonium bromide) extraction takes a long time with excess impurity is overcome.

The invention discloses a nocardia rubra-cell wall skeleton solvent extraction method which is characterized by including the steps: (1) ethyl acetate extraction: adding ethyl acetate in nocardia rubra-cell wall skeleton fragments acquired after enzymolysis, stirring mixture for 5-18 hours at the indoor temperature, performing centrifuging treatment, abandoning liquid supernatant, and reserving sedimentation fragments; (2) absolute ethyl alcohol stirring and cleaning: adding the absolute ethyl alcohol into the sedimentation fragments acquired in the step (1) to uniformly stir mixture, performing centrifuging treatment, abandoning liquid supernatant, and reserving sedimentation fragments; (3) absolute ethyl alcohol extraction: adding the absolute ethyl alcohol into the sedimentation fragments acquired in the step (2), stirring mixture for 5-18 hours at the indoor temperature, performing centrifuging treatment, and abandoning liquid supernatant to obtain final sedimentation fragments. The proportion of the nocardia rubra-cell wall skeleton fragments and ethyl acetate is 1g: 5-8ml, and the proportion of the sedimentation fragments acquired in the step (2) and the absolute ethyl alcohol is 1g:5-8ml.

The invention relates to a nucleotide specific to wzt of Legionella pneumophila serogroup 1 and application thereof. The nucleotide is a nucleotide shown as SEQ ID NO: 1 and / or a nucleotide shown as SEQ ID NO: 2 and is complementary with the nucleotides. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Legionella pneumophila serogroup 1, a gene chip or a micro-array. The nucleotide specific to the wzt of the Legionella pneumophila serogroup 1 provided by the invention as well as the PCR kit, the gene chip or the micro-array including the nucleotide have the advantages of strong practical applicability, simple preparation method of the PCR kit, short detection period, high speed, strong maneuverability, easiness for industrial production, low detection cost, high accuracy and high sensitivity.

The invention provides a magnetic microsphere and application thereof in hair detection. After cleaned to-be-detected hair is ground, the magnetic microsphere is added and evenly mixed, and the mixture is dried after magnetic separation; then eluant is added, and the materials are evenly mixed and then subjected to secondary magnetic separation treatment; finally, supernate is obtained for liquidchromatography mass spectrometry to detect the content of oxazepam drugs. The magnetic solid-phase extraction technology is used as a pretreatment technology, so that the influence of human factors isreduced, the extraction step of the oxazepam drugs for hair is improved, the organic solvent consumption is low, the magnetic microsphere is more environmentally friendly, and the sensitivity is high.

The invention provides an excrement nucleic acid methylation purification method. The method includes the following steps that 100 ul of an excrement solution is provided into a centrifugal tube; 1 mL of lysate is added into the centrifugal tube, wherein the lysate is prepared from 0.3N sodium hydroxide, 0.4M potassium chloride, 0.025%N sodium lauroyl sarcosine, 2mM EDTA, 0.4M Tris HCL and 1.5% triton X-100; 100 ul of a conversion solution with the ph value being 5.5 is added into the centrifugal tube, and the centrifugal tube is put in an environment at the constant temperature of 75 DEG C for 90 Min; after the material is cooled to room temperature, 10 ul of a magnetic bead solution is added, and the material is mixed to be uniform and put for 15 Min; the centrifugal tube is put on a magnetic frame for 5 Min, and clean liquid is removed; the second step to the six step are repeated till magnetic beads are separated, and supernate is transferred into another centrifugal tube.

The invention discloses a method for efficiently and quickly extracting crop genome DNA (DeoxyriboNucleic Acid). The method specifically comprises the following steps: putting a root system, a seedling or a leaf which grows after the single seed of a crop sprouts into a container, and adding an alkaline solution and steel balls to carry out grinding pyrolysis so as to obtain homogenate; adding Tris buffered solution into obtained lysate, and collecting upper-layer clear solution. Compared with a traditional alkaline pyrolysis method, the method disclosed by the invention is characterized in that TritonX-100 solution or NP40 solution are added in an alkaline grinding pyrolysis process to more simply and quickly extract DNA, extraction quality is higher, and an extraction effect is better. Since the Tris buffered solution is added, an influence for subsequent PCR (Polymerase Chain Reaction) since the pH (Potential of Hydrogen) value of the extracted DNA solution is overhigh or overlow can be avoided, and only two solutions need to be added to extract high-quality DNA; a high-temperature water bath process is omitted, and the efficient extraction of the DNA can be realized only through two steps. The method established by the invention is suitable for the quick detection of the purity, the authenticity and the transgenic ingredients of the single seed of the crop and the auxiliary selection research of the key character molecular marker of the crop.

A process for extracting the water-soluble components from red-sage root and purifying it features the combination of water-extracting and alcohol deposition method, acidic deposition method, and alcohol solution pH-regulating method. Its advantages are low cost, high speed and high purity effect.

The invention involves the veterinary medicine which takes heartleaf houttuynia herb as raw materials and has the function of clearing away the heat-evil and expelling superficial evils, detumescence and purulent discharge and its preparation method. After refining the heartleaf houttuynia herb medicinal substances, wash and cut it into pieces, then extract the ground heartleaf houttuynia herb: extract it with eight times the weight of water of the medicine, the extraction temperature is 95 +- 3DEG C, accommodate its pH to 2-5 with 10% acid for the first extraction, decocte for 2 hours; still add the water to eight times the weight of the medicine for the second extraction and accommodate its pH to 8-9 with 10% alkali, 1.5 hours extraction. Merge the physic liquor after they are filtrated respectively, after drying, pulverization heartleaf houttuynia herb refined powder is obtained.

The invention relates to a composition medicine used for curing senile dementia and a preparation method thereof, which uses ginkgo leaves and salvia miltiorrhiza as raw materials. The raw materials are processed by soaking for extraction at low temperature, refining of column absorption, cooling and drying for obtaining extracts; then appropriate amount of pharmaceutic adjuvant are added for preparing pharmaceutical preparations of formulations allowed by various pharmaceutics by the common technique. The invention has explicit pharmic composition, advanced technique and definite curative effects; the medicine prepared by the invention and used as the medicine for providing nutrition to protect cranial nerves and preventing degenerative brain aging has obvious effects; the effects of the medicine are obviously stronger than using the ginkgo leaves or the salvia miltiorrrhiza alone; the compatible medication has addition and synergetic effect; the medicine has excellent effects on curing the senile dementia, learning and memory dysfunction and the brain aging, etc.

The present invention relates to an animal Chinese medicine loquat leaf powder with the functions of removing heat from the lung and dissolving phlegm, regulating the stomach and downbearing fire for curing animal diseases. It is made up by using Chinese medicinal material loquat leaf as raw material through a certain preparation process. Besides, said invention also provides the concrete steps of said preparation process.

The invention provides a method for preparing chiral alpha-hydroxy acid by double bacterium coupled catalysis of racemic alpha-hydroxy acid, comprising the following steps: using an alpha-hydroxy acid raceme of formula (I) as a substrate and using (S)-alpha-hydroxy acid dehydrogenase and (R)-keto acid reductase as a catalyst, reacting in a buffer with the pH value of 6.0-9.0 at 20-50 DEG C for 10-48 h to prepare the corresponding (R)-alpha-hydroxy acid. According to the invention, the chiral alpha-hydroxy acid is synthesized by using the racemic alpha-hydroxy acid as the substrate and carrying out deracemization through biotransformation, thus the reaction conditions are mild, the environmental pollution is low, the extraction step of intermediates is omitted, and the yield and enantiomerexcess are high, wherein the yield reaches more than 90% and the enantiomer excess reaches more than 99%.

The invention discloses a method for processing sesame oil by a stone mill. The method comprises the following steps: selecting raw material sesame seeds, and immersing and washing the selected raw material sesame seeds; feeding the immersed and washed sesame seeds into a vacuum device, carrying out vacuum drying, and carrying out hot air low-temperature drying on the sesame seeds; and feeding thedried sesame seeds into an ultrasonic crusher, crushing the sesame seeds, and heating the crushed sesame seeds. The method for processing sesame oil by the stone mill can extract sesame oil at a lowtemperature, and can avoid denaturation of sesame proteins, caused by excessive temperature, so no loss of the nutrient components of the sesame oil is ensured, and the original extraction step of sesame oil is reduced; the method can reasonably control the temperature of the sesame seeds during grinding of the stone mill, so the oil yield of the sesame seeds is high; and the ultrasonic crushing is combined to reduce the difficulty in the grinding of the stone mill and prolong the service life of the abrasion pattern of the stone mill, so the method brings a good use prospect.

A veterinary medicine with the functions of stypsis, haemostasis, treating dycentery and detoxication for treating the epidemic diseases of domestic animals and fowls is prepared from grinony root through pre-treating, decocting in water for 2 hr while using acid to regulate pH=2-5, filtering, decocting again for 1.5 hr while using alkali to regulate pH=8-9, filtering, collecting the decoction, drying and pulverizing.

The invention relates to the technical field of virus detection. The invention provides a rapid detection technology of high-sensitivity pathogenic nucleic acid for non-diagnosis and treatment purposes. The rapid detection technology comprises the following steps of (1) carrying out isothermal amplification on recombinase; and (2) carrying out color developing by colloidal gold. According to the rapid detection technology for the high-sensitivity pathogenic nucleic acid, isothermal nucleic acid amplification-colloidal gold color development is used for pathogen field detection, special equipment and reagent are not needed, meanwhile, the pathogen extraction step is omitted, pathogen nucleic acid signals can be captured and amplified to the maximum extent, sputum, blood, excrement and othersamples of a patient can be directly detected, and the technology is very suitable for field detection, bedside diagnosis of hospitals, crowd gathering and living scenes such as schools and airportsand application under the condition of few other resources, and is particularly suitable for scenes where results need to be obtained rapidly. The defects that in the prior art, pathogen nucleic aciddetection depends on large equipment and reagent, the detection period is long, the sensitivity is low, and pathogen needs to be extracted can be overcome.

The present invention relates to the preparation process and refining method of solano bromide. Solano bromide is prepared with solanesol as initial material and the preparation process includes the main steps of bromination with phosphorus tribromide in non-polar solvent and subsequent direct washing with polar solvent to refine. The said refining method has no extraction, drying, chromatographic column refining and other steps and thus has no emulsification, decomposition, so that the present invention has high product yield and purity and lowered cost.