10 results about "Ethidium bromide" patented technology

Methods are provided for the isolation and analysis of circular DNA from complex samples, based on the topology of the DNA molecule. A sample comprising DNA species is combined with a chaotropic dense salt solution. A fraction containing the circular DNA of interest is isolated and dialyzed to remove excess salt. In some embodiments salt gradients are generated by ultracentrifugation in the absence of intercalating dyes, e.g. ethidium bromide; and in the absence of protease digestion. The circular DNA thus isolated is substantially pure, e.g. greater than about 75%, greater than about 80%, greater than about 90%, greater than about 95% of DNA in the isolated fraction is comprised of circular DNA.

The application discloses an application of a light-cured material in safety indication or metal flaw detection and a safety indication and metal flaw detection method, and the light-cured material is prepared by uniformly mixing raw materials including an organic fluorescent dye and a light-cured glue; the organic fluorescent dye is a mixture of one or more of quinacridone, acridine yellow, acridine yellow G, acridine yellow hydrochloride, sulfuric acid proflavine, acridine orange, acridone, ethidium bromide and 9-aminoacridine; the light-cured glue is a mixture of one or more of UV drop glue D-606, ultraviolet curing adhesive 3491, ultraviolet curing adhesive YY1181, V3218 and ultraviolet curing adhesive 3492; or, the organic fluorescent dye is a mixture of one or more of acridine yellow, sulfuric acid proflavine, acridine orange and 9-aminoacridine, and the light-cured glue is V3018. The light-cured material can emit afterglow under white light excitation after curing, and can be applied to large-area safety indication and metal flaw detection.

The application discloses application of a zwitterionic covalent organic framework solid-phase microextraction fiber in neurotransmitter analysis, wherein the zwitterionic covalent organic framework material is prepared by Schiff base reaction condensation with ethidium bromide, 2,5-diaminobenzoic acid and triformylphloroglucin as functional monomers and acetic acid as a catalyst to obtain the zwitterionic covalent organic framework material. The coating of the zwitterionic covalent organic framework solid-phase microextraction fiber prepared in the application has good hydrophilicity, and the structure is rich in functional groups such as hydroxyl groups, carboxyl groups, phenyl groups and quaternary ammonium groups, which can interact with target analytes through ion exchange, pi-pi stacking, hydrogen bonding and hydrophilic-hydrophobic interaction, thereby showing strong specific adsorption and high enrichment capacity for neurotransmitters. The zwitterionic covalent organic framework solid-phase microextraction fiber prepared in the application realizes efficient enrichment and extraction analysis of neurotransmitters with different polarities in complex biological sample matrices.

The present invention discloses a two-dimensional monolayer ionic COF and its application in osmotic energy conversion. The two-dimensional monolayer ionic COF membrane is obtained by polymerizing 5,10,15,20-tetrakis (4-formaldehyde benzene)-21H, 23H-porphyrin and ethidium bromide to form an imine covalent bond through Schiff base reaction; the thickness is 0.9-1.1nm and the pore size is 1.6-1.8nm. The two-dimensional s-iCOF material of the present invention is formed by covalently linking 5,10,15,20-tetrakis (4-formaldehyde benzene)-21H, 23H-porphyrin and ethidium bromide, with larger porosity, good integrity, regular pore arrangement and positive charge. In osmotic energy conversion, larger porosity and regular pore arrangement ensure considerable ion flux, good integrity and the properties of the monolayer ensure its high energy conversion rate, and the positive charge surface indicates that ions can be selectively transmitted in order to seek a larger output potential. In summary, extremely excellent output power can be achieved by osmotic energy conversion using two-dimensional s-iCOF.

The application discloses a Toxoplasma SRS29C and ROP16 double-gene deletion strain and a construction method and application thereof, relates to the technical field of parasite genetic engineering and molecular biology. The Toxoplasma SRS29C and ROP16 double-gene deletion strain is obtained by knocking out SRS29C and ROP16 genes of the ME49 strain through a CRISPR / Cas9 system. The double-gene deletion strain constructed by the application has drug sensitivity change (sensitivity to ethidium bromide), growth defect phenotype, and only carries a single screening marker, and under in-vitro culture conditions, the double-gene deletion strain presents a significant slow growth trend, and the number of worm-containing bubbles is obviously less than that of the wild type and single-gene deletion strain. The double-gene deletion strain substantially influences the proliferation of the worm body, and provides key data support for exploring the molecular basis of the double-gene deletion leading to the increase of the drug sensitivity of the worm body and for analyzing the regulation mechanism of the drug tolerance related path of the Toxoplasma.

The application discloses a kind of covalent organic framework material solid-phase microextraction fiber and its application in alkaloid metabolism and neurotransmitter analysis.The covalent organic framework material (COFs) includes the repeating structural unit of 4,4',4"-(1,3,5-triazine-2,4,6-triazyl) tri[benzaldehyde] connected by imine bond, ethidium bromide and 2,5-diaminobenzenesulfonic acid.The COFs material is used as the adsorbent of solid-phase microextraction fiber surface coating, and the COFs is coated on the surface of stainless steel fiber by polyacrylonitrile glue.The COFs solid-phase microextraction fiber of the application can be used to extract rhizoma coptidis alkaloids and neurotransmitters in the brain and blood of living rats, and after sampling extraction, it is directly combined with LC-MS / MS for mass spectrometry analysis, with very high sensitivity, and can be used for rapid, accurate and sensitive analysis of trace rhizoma coptidis alkaloid metabolites and neurotransmitters in vivo.

The invention provides a method and equipment for extracting alkali metal from lithium precipitation waste. Relates to the field of alkali metal extraction, 0.0003 mol of ethidium bromide and 0.0002 mol of 1, 3, 5-triformyl phloroglucinol are respectively added into 7.5 mL of dimethyl sulfoxide, and ultrasonic treatment is performed for 10 minutes to obtain an aldehyde monomer solution and an amine monomer solution; and S2, slowly dropwise adding the aldehyde monomer solution into the amine monomer solution, stirring until the aldehyde monomer solution becomes dark red, and standing for 24 hours to obtain the porous organic framework nanosheets, the artificial cation-chloride ion co-transporter membrane has a porous organic framework with weak surface charges and narrow pores and high selectivity, and can be used for preparing the artificial cation-chloride ion co-transporter membrane. Basic ions and halogen ions Cl <-> can be identified, other cations and anions are repelled, and the size-induced ion selectivity is Cl <-> approximately equal to K + gt; na + gt; li + gt; gt; ca < 2 + > gt; mg < 2 + > has low permeability to Rb and Cs, the lithium ion permeability and the lithium extraction performance are improved by utilizing rich chloride ions in the lithium precipitation mother liquor, excessive waste liquid is not easy to generate, the selectivity is good, the process is fast, and the cost is low.

The invention relates to the technical field of antitumor drugs, in particular to application of a TFAM inhibitor in preparation of antitumor drugs. It is found for the first time that all reagents which take TFAM as a target spot and can inhibit TFAM gene or protein expression or inhibit TFAM gene or protein functions can affect expression of mitochondrial genes in cells, the mitochondrial functions are remarkably reduced, then cancer cell apoptosis is selectively induced, and tumor growth and migration are effectively inhibited; meanwhile, the ethidium bromide targeting TFAM can effectively remove tumors (such as colorectal tumor tissues) in vivo, and also has a very good inhibition effect on cancer cell proliferation and migration (such as migration of pancreatic cancer cells in pancreas, liver and lung tissues); more importantly, the invention finds that the side effect of the ethidium bromide targeting the TFAM is very small. Therefore, TFAM serving as a target for development of antitumor drugs has extremely high clinical application value, and clinical experiments and application thereof are expected to be promoted.

The application discloses a kind of monovalent selective cation exchange membrane containing covalent organic framework modification layer and preparation method and application thereof.The preparation method comprises the following steps: (1) ethidium bromide monomer, catalyst is dissolved in deionized water to obtain aqueous solution;(2) aldehyde monomer is dissolved in organic solvent, to obtain organic phase solution;(3) sulfonated polyether ether ketone-based film is immersed into aqueous solution, after taking out, film surface water droplet is blown dry, then immersed into organic phase solution, take out and air dry, obtain monovalent selective cation exchange membrane containing covalent organic framework modification layer.The application provides the application of the monovalent selective cation exchange membrane containing covalent organic framework modification layer in electrodialysis separation single multivalent cation.Under the condition of ensuring that the monovalent ion of the membrane has higher permeation flux, the membrane surface resistance is small, the tensile strength of the membrane is improved, the water absorption of the membrane is reduced, and the single multivalent selection performance of the membrane is significantly improved.

The invention provides a fungus multi-fluorescent dyeing preparation and a preparation method and application thereof.The dyeing preparation comprises a solution A and a solution B. The solution A is mainly prepared from, by mass, 0.6-1 part of CYP51 fluorescent dye, 5-10 parts of fluorescent whitening agent, 3-4 parts of potassium hydroxide, 0.2-1 part of dimethyl sulfoxide and a proper amount of glycerin; the liquid B is mainly prepared from the following components in parts by mass: 0.05 to 0.2 part of ethidium bromide, 0.05 to 0.2 part of acridine orange, 0.5 to 2 parts of calcium fluorescent white, 7 to 10 parts of propidium iodide, 0.3 to 1 part of dimethyl sulfoxide and a proper amount of glycerol. According to the fluorescent staining preparation, the staining accuracy is improved, and dead bacteria and drug-resistant bacteria in fungi can be better stained.