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SNP (Single Nucleotide Polymorphism) molecular marker for detecting sterile gene pms3 (photoperiod-sensitive male sterility 3) of paddy rice

A molecular marker and rice technology, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of unintuitive result verification, cumbersome primer design, and affecting genotyping efficiency, so as to avoid health problems. Threats, solutions to long detection cycles, and the effect of reducing investment costs

Inactive Publication Date: 2018-05-18
HUAZHI RICE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the primer design of this method is cumbersome, and the result verification is not intuitive enough, which affects the efficiency of genotyping

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker for detecting sterile gene pms3 (photoperiod-sensitive male sterility 3) of paddy rice
  • SNP (Single Nucleotide Polymorphism) molecular marker for detecting sterile gene pms3 (photoperiod-sensitive male sterility 3) of paddy rice
  • SNP (Single Nucleotide Polymorphism) molecular marker for detecting sterile gene pms3 (photoperiod-sensitive male sterility 3) of paddy rice

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 common rice variety detection

[0041] The marker K_120522 was used to verify the KASP primary screening reaction of 8 gene donor materials including rice varieties GD-1S, 7001S, and Peiai 64S containing the pms3 gene and 27 other rice varieties without the restorer gene. The results are shown in Table 3. The rice varieties containing the pms3 gene were all detected as G-type bases at the marker test sites, and the base C was detected at the test sites by 27 non-donor rice varieties, which proved that the molecular marker K_120522 and its primers provided by the present invention The group detection results are accurate and specific.

[0042] Table 3: Marker K_120522 Test Genotyping Data

[0043]

[0044]

[0045]

Embodiment 2

[0046] Embodiment 2 Natural population detection

[0047] SNP marker K_120522 was validated by natural population using 95 materials. The 95 materials include 34 common hybrid rice materials and 60 core rice breeding materials. Marked in natural group typing results such as figure 1 As shown, 95 materials were clearly classified. Except for the homozygous pms3 detected in the core rice breeding material Guangxiang 24S, 34 common rice hybrid varieties and the remaining 59 materials were tested to be free of pms3, which is in line with the application of the pms3 gene in actual breeding. The current situation that is still lacking has verified the feasibility and accuracy of the SNP molecular marker detection method developed by the present invention, which can be used for identification of pms3 gene and assisted selection breeding.

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Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker for a sterile gene pms3 (photoperiod-sensitive male sterility 3) of paddy rice. The molecular marker is K-120522, wherein the polymorphism of the K-120522 is C or G. By utilizing the molecular marker, the gene pms3 can be quickly and precisely detected; the selection efficiency, in the breeding of the paddy rice, of the gene is greatly improved. The enzyme digestion, the electrophoresis, the sequencing and the like are not needed in a detection process; the operation is simple and convenient; the commercialized high-throughput quick detection can be realized; the aerosol pollution of a PCR (Polymerase Chain Reaction) product, the pollution of EB (Ethidium Bromide) to an environment and the harm of formaldehydeto a human body are thoroughly avoided.

Description

Technical field: [0001] The invention relates to the field of molecular markers, in particular to a SNP molecular marker of a rice sterility gene. Background technique: [0002] Rice is an important food crop, and the promotion of hybrid rice is an important way to increase rice yield. In two-line hybrid rice breeding, there are mainly two types of sterile lines: temperature-sensitive and light-sensitive. The temperature-sensitive sterile line plays an important role in actual production. Nongken 58S is a photo-temperature-sensitive male sterile rice variety. It is sterile under long-day high temperature and fertile under short-day low temperature. It is a two-line rice breeding method. important germplasm resources. The traditional breeding of two-line male sterile lines observes the pollen fertility of rice and selects sterile plants in the field at the heading and flowering stage. It needs to go through pollen microscopic examination, bagging self-crossing, cutting and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 彭佩贺治洲张先文吴云天
Owner HUAZHI RICE BIO TECH CO LTD
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