54 results about "Single-nucleotide polymorphism" patented technology

The application provides a peanut germination period cold tolerance related KASP molecular marker, a primer group and an application thereof, and belongs to the technical field of molecular markers.The RIL population constructed by using the cold tolerance variety Sihelianhong and the low temperature sensitive variety Jinnonghei No.3 as materials, a SNP site closely related to the germination period cold tolerance is developed at the Chr.18:2374669 site of the peanut 18th chromosome, and the site has A / C single nucleotide polymorphism.The application also designs a primer group for detecting the SNP site, and the primer group comprises a first upstream primer, a second upstream primer and a universal downstream primer.The primer group can quickly identify the peanut germination period cold tolerance, has the advantages of accuracy, rapidness, low cost, short identification period, simple operation and the like, and provides an efficient molecular detection tool for the peanut planting industry in high latitude and low temperature areas.

This invention discloses a molecular marker for superovulation in cattle using the EXOC4 gene and its application method, belonging to the field of animal genetic engineering technology. By conducting correlation analysis between EXOC4 gene polymorphism and the effect of superovulation in cattle, the single nucleotide polymorphism at a specific site of the EXOC4 gene and its accurate identification method were clarified, and the influence of this site's genetic polymorphism on the effect of superovulation in cattle was determined. This provides an important theoretical basis and application prospect for using it as a molecular marker for auxiliary selection of bovine reproductive performance and applying it to genetic improvement in actual production.

The application discloses a wheat arabinoxylan content related molecular marker and application thereof. The application belongs to the technical field of biology, and provides a wheat genome SNP molecular marker and a substance for detecting the molecular marker, which are applied to any one of the following: (1) identifying or assisting in identifying wheat water-soluble arabinoxylan content; (2) wheat breeding; (3) preparing a product for identifying or assisting in identifying wheat water-soluble arabinoxylan content; and (4) preparing a wheat breeding product. The substance for detecting polymorphism and genotype of the above-mentioned SNP can be combined with other substances (such as a substance for detecting single nucleotide polymorphism or genotype of other wheat water-soluble arabinoxylan content related molecular markers) to prepare a product for identifying a wheat variety with wheat water-soluble arabinoxylan content, to perform early and efficient auxiliary screening on WE-AX content, and to improve breeding efficiency.

The application discloses a modified crRNA, a light-controlled nucleic acid detection system, a kit and application, and belongs to the cross field of biotechnology, intelligent sensing and molecular diagnosis. In view of the technical defects of strong target sequence dependence and high ultraviolet irradiation requirement of the existing light-controlled CRISPR technology, the application innovatively introduces a photosensitive protection group 6-nitropiperidin oxymethyl (NPOM) at a specific key node of a stem loop skeleton of crRNA maintaining conformation. In the constant temperature amplification stage, preferred double-site cooperative modification can transiently inhibit RNP complex assembly to realize target non-interference enrichment; subsequently, only 10 mW / cm 2 of extremely low intensity ultraviolet light irradiation for 30 seconds can restore the crRNA conformation and activate the trans cleavage. The preferred technical scheme of the application can eliminate the target sequence limitation, realizes a detection limit of as low as 2 copies in a single reaction tube, and the result can be obtained within 15 minutes. The system is widely applicable to rapid diagnosis of infectious agents, high-specificity typing of single nucleotide polymorphism and portable intelligent molecular diagnosis terminal.

The application provides a SNP molecular marker related to a multiple lamb trait of sheep, a detection primer set, a kit and application thereof, and belongs to the technical field of SNP molecular markers. The application provides application of the SNP molecular marker related to the multiple lamb trait of sheep in detection of the multiple lamb trait of sheep or sheep breeding. ABCA1 The application provides a method for detecting single nucleotide polymorphism of ABCA1 gene of sheep and application thereof, and provides basic data for molecular assisted marker selection breeding of sheep and accelerates improvement of Chinese sheep germplasm resources.

The present invention relates to methods for determining the risk of developing a liver disease based on single nucleotide polymorphism detection. More specifically, the present invention comprises the use of rs3859093, rs34474737, rs10893, rs2070666, rs7087728, rs55907967 and / or rs17846713. The present invention therefore relates to the field of biomedicine.

The application belongs to the technical field of biology and specifically relates to a Cas12a protein mutant with high cleavage activity and application thereof. The Cas12a protein mutant has high trans-cleavage activity and has a good application prospect in nucleic acid detection. The Cas12a protein mutant changes the binding of the protein and crRNA by directional evolution of the RECII domain, improves the cleavage efficiency of the target strand in double-stranded DNA, and improves the detection sensitivity, and can distinguish single nucleotide polymorphism (SNP). The Cas12a protein mutant can hardly depend on PAM sequence recognition for SNP site detection, can distinguish SNP located between positions 1 to 17, and realizes high-sensitivity and high-precision molecular detection.

The application discloses a method and application of a Cas12a sensing system based on an sgRNA blocking strategy for single nucleotide polymorphism typing. The method comprises the following steps: pre-annealing sgRNA and blocking chains to form a complex; obtaining a single-stranded DNA target by using an asymmetric recombinase polymerase amplification; constructing a detection system comprising a Cas12a protein, the complex, a target and a fluorescent substrate; activating the trans-cleavage activity of Cas12a through a strand displacement reaction to generate a fluorescent signal, so as to realize SNP typing. The method combines the sgRNA blocking strategy with the Cas12a system for the first time, does not need to depend on a PAM sequence, has the advantages of high universality, high specificity, simple operation, rapidness, low cost and the like, and is suitable for ApoE gene typing and other SNP related detection.

The present application relates to a kind of detection methods of strong putrefaction Pseudomonas lundensis, comprising extracting nucleic acid in the sample to be measured;Then with nucleic acid as template, using specific RAA primer pair is recombined enzyme-mediated amplification, and obtains amplification product;RAA primer pair targets the aprX gene of Pseudomonas lundensis comprising single nucleotide polymorphism site associated with strong putrefaction phenotype;Finally, the amplification product is added to CRISPR / Cas detection system, incubated at constant temperature, then real-time detects its fluorescence signal;Detection system includes Cas protein, specific crRNA and fluorescent reporter probe, and crRNA can recognize single nucleotide polymorphism site associated with strong putrefaction phenotype in Pseudomonas lundensis aprX gene.The present application improves the detection accuracy, and detection process is simple to operate, and reaction system is simple, not only improves detection efficiency, also reduces detection cost.

This invention belongs to the field of plant molecular biology and genetic breeding technology, specifically relating to a molecular marker related to the malic acid content of apple fruit and its application. The molecular marker is... MdNAC029 The single nucleotide polymorphism site in the gene promoter region, this site is located MdNAC029 The nucleotide sequence of the gene promoter region, starting from the 2693rd base at the 5' end, corresponds to... MdNAC029 At a position 442 bp upstream of the transcription start codon, the polymorphism is either T or C, with the C / C genotype indicating high malic acid content and the T / T genotype indicating low malic acid content. This invention, through molecular biology experiments, confirms that the T allele is the binding site for the transcriptional repressor MdMYC2, and the C allele eliminates the binding of MdMYC2, thereby relieving transcriptional repression and promoting malic acid accumulation. This provides a new molecular marker for the genetic improvement of apple fruit acidity traits, accelerating the breeding process of superior varieties.

The present invention relates to a vascular endothelial growth factor (VEGF) inhibitor for use in the treatment of wet macular degeneration, wherein the VEGF inhibitor is adapted to be administered intravitreally to a patient, wherein the patient has previously been treated intravitreally with the VEGF inhibitor for approximately one year, and has one or more genetic variants that are single nucleotide polymorphisms selected from rs2106124, rs1879796, rs12148845, rs12148100, rs17482885, and rs17629019.

The application discloses a SNP molecular marker for identifying the content of wheat arabinoxylan and application thereof. The application belongs to the technical field of biology and specifically comprises detecting the polymorphism or genotype (allele) of an SNP site in a wheat genome to be measured, and identifying or assisting in identifying the content of wheat arabinoxylan according to the genotype, wherein the SNP site is a site on a 4D chromosome of wheat, the nucleotide type of which is A or G, and the 36th nucleotide in sequence 1 in a sequence table. The application can be used for predicting the content of wheat arabinoxylan and for wheat breeding. The substance for detecting the polymorphism and genotype of the above SNP site can be combined with other substances (such as a substance for detecting a single nucleotide polymorphism or genotype of another molecular marker related to the content of wheat arabinoxylan) to prepare a product for identifying wheat arabinoxylan with high content.

Disclosed herein are systems and methods for performing secondary analysis of nucleotide sequencing data in a time-efficient manner. Some embodiments include iteratively performing secondary analysis as sequence reads are generated by a sequencing system. Secondary analysis can include alignment of sequence reads to a reference sequence (e.g., a human reference genome sequence) and use of the alignment to detect differences between a sample and the reference. Secondary analysis can be capable of detecting genetic differences, variant calling and genotyping, identifying single nucleotide polymorphisms (SNPs), small insertions and deletions (indels), and structural changes in DNA, such as copy number variations (CNVs) and chromosomal rearrangements.

This invention relates to the field of animal genetic engineering technology, providing a molecular marker for the ATP5PO gene associated with bovine sperm motility and its application. The ATP5PO gene molecular marker is located at position 203 of the bovine ATP5PO gene fragment, where an A-G base mutation occurs, resulting in a single nucleotide polymorphism (SNP). The nucleotide sequence of the bovine ATP5PO gene fragment is shown in SEQ ID NO:1. Based on this ATP5PO gene molecular marker, genotype can be determined through sequencing, and the genotype can be associated with normal and thawed bovine sperm motility. This invention utilizes a specific primer set to obtain the bovine ATP5PO gene fragment associated with normal and thawed bovine sperm motility, and uses specific SNP sites within this fragment as molecular markers, providing a theoretical basis and practical application for marker-assisted selection in cattle.

The present application provides a kind of embryo relationship determination method, device, computer equipment, storage medium and program product, the method comprises: embryo is carried out before embryo implantation aneuploid gene detection shallow sequencing, obtains embryo sequencing FASTQ data;The FASTQ data of embryo sequencing is mixed, and the FASTQ file of each pair of sample is obtained;The FASTQ file of each pair of sample is preprocessed, and single nucleotide polymorphism site information is obtained;The likelihood ratio of sibling relationship is calculated using single nucleotide polymorphism site information.The method can be realized in the scene of shallow sequencing, the likelihood ratio of sibling relationship is calculated to determine the relationship of embryo, the method can accurately determine the relationship of brother and sister of embryo, assist medical staff to select correct embryo, the method is also applicable to the relationship of embryo and parent determination.

This invention belongs to the field of molecular biology, specifically relating to specific primers for molecular markers of the fertility-related gene HIRA in Ujumqin sheep and their applications. The specific primer sequences for the molecular markers of the fertility-related gene HIRA in Ujumqin sheep are shown in SEQ ID NO.1 and SEQ ID NO.2. This invention designs specific primers to detect the presence of a G→A mutation at 1273 bp in the coding region of the HIRA gene in the Ujumqin sheep genome, determining the genotype of individual Ujumqin sheep at this locus, and realizing the detection of single nucleotide polymorphism (SNP) c.1273G>A in the HIRA gene to compare the polymorphism of HIRA gene c.1273G>A in the Ujumqin sheep breed. This invention utilizes the HIRA gene c.1273G>A polymorphism to assist in the breeding of Ujumqin sheep, increasing the number of lambs born, and can serve as an effective method to assist in improving the multiparity trait of Ujumqin sheep.

The application relates to the technical field of animal gene detection, and discloses an application method of using an IL1RAP gene as a molecular marker of a superovulation trait of a cow, which comprises the following steps: extracting a genomic DNA template from blood of a to-be-detected cow individual; using a forward primer and a reverse primer to combine the template to perform PCR amplification, obtaining an amplified fragment to obtain a PCR product; performing sequence determination on the PCR product, detecting single nucleotide polymorphism caused by a C to T base mutation existing at the 198th position of a DNA sequence to obtain a target genotype; and performing correlation analysis on the target genotype and the superovulation trait of the cow, and selecting the to-be-detected cow individual with the TT type when the target genotype is the TT type. The application realizes the application of the IL1RAP gene as the molecular marker by detecting single nucleotide polymorphism of the amplified fragment, performing correlation analysis on the target genotype and the ovulation trait, and screening the TT type individual, and the influence of the polymorphism on superovulation is determined.

The invention relates to a diagnostic microarray device comprising: a substrate, preferably formed of a glass material; wherein a plurality of probes attached or otherwise associated with the substrate, each probe being specific to one allele of a single nucleotide polymorphism (SNP), said SNR being selected from the group consisting of IL1B+3877, IL1B-511, and IL6-1363, and preferably IL10-592, CD14-260 and COX2+8473.

The present application relates to a kind of molecular marker set for egg duck breeding, the molecular marker set includes multiple single nucleotide polymorphism (SNP) sites, the multiple SNP sites are determined based on the physical location of egg duck reference genome ZJU1.0, and the multiple SNP sites include functional SNP site and background SNP site;Wherein, the functional SNP site is significantly related to the reproductive trait phenotype of egg duck, the background SNP site is screened based on the principle of uniform distribution of genome.The set of the present application is directly associated with important breeding target traits, improves the accuracy of genomic selection, ensures that the genetic information in the whole genome range is evenly captured, enhances the robustness of breeding value estimation and the coverage of whole genome genetic variation.

The present invention is directed to salinity and / or sodicity tolerant plants. Specifically, methods for producing plants having salinity and / or sodicity tolerance are provided, together with plants produced therefrom. The present invention is also directed to genetic markers (including single nucleotide polymorphisms and genes) associated with salinity and / or sodicity tolerance in plants, as well as methods for identifying plants using said markers. Methods for increasing the salinity and / or sodicity tolerance of a plant are also encompassed herein. Furthermore, the present invention provides wheat plants having salinity and / or sodicity tolerance, as well as salinity and / or sodicity tolerant wheat germplasm and uses thereof.

The application belongs to the technical field of animal genetic engineering, and provides a FETUB gene molecular marker related to the sperm vigor trait of a cow and application thereof, wherein the FETUB gene molecular marker is an SNP site on a FETUB gene fragment of the cow as shown in the sequence table SEQ ID NO:1, specifically, the 315th site of the FETUB gene fragment, the site has an A-G base mutation, resulting in the generation of a single nucleotide polymorphism of the gene. Based on the FETUB gene molecular marker, the genotype is determined by sequence determination, the genotype is associated with the normal and post-thawing vigor traits of the sperm of the cow, and the normal and post-thawing vigor of the sperm of individuals with different genotypes has a significant difference. The FETUB gene molecular marker provided by the application provides a theoretical basis and specific application for marker-assisted selection of the cow.

The application discloses a SNP (Single Nucleotide Polymorphism) related to soybean plant height under shade-avoidance reaction and application thereof, wherein the SNP site is located at the 248th base from the 5' end of the nucleotide sequence shown in SEQ ID NO:1; the site contains two allelic types: T type and A type; soybeans with different genotypes have different plant heights, specifically: at least in a weak light environment, the plant height of soybean with the genotype of TT homozygote is greater than or candidate greater than that of soybean with the genotype of AA homozygote. The technical research result of the application is expected to be used in molecular marker assisted breeding of soybean and related crops, and has important values in the aspects of theory and application for breeding of soybean varieties suitable for close planting, enhancement of plant resistance to lodging, increase of soybean yield and other practical hot issues.

The present application belongs to the technical field of tumor genetic detection, and particularly relates to a reagent, a kit, a chip, a system for detecting a SNP site of a CLDN18 gene and application thereof. Based on genome-wide association analysis (GWAS), the present application identifies a single nucleotide polymorphism (SNP) site related to the pathogenesis of lung cancer, and combines fine mapping, co-localization and functional experiments to first confirm that rs6804932 of the CLDN18 gene is a potential lung cancer protective variation. The constructed lung cancer risk assessment model shows that, after combining age, gender and smoking status, the inclusion of rs6804932 can make the model AUC reach 0.712, having good risk prediction performance, which indicates that the site can be used as an important genetic marker for lung cancer risk prediction. Therefore, the rs6804932 site of the CLDN18 gene has good application prospects in lung cancer genetic susceptibility detection, risk assessment and preparation of related detection products.

The present application belongs to the technical field of molecular markers, and particularly relates to a tartary buckwheat stalk strength molecular marker and application thereof in identifying tartary buckwheat stalk strength traits. The molecular marker provided by the present application is located at 35,829,660 bp on the first chromosome of tartary buckwheat, and the single nucleotide polymorphism is C / G; when the base is C, the tartary buckwheat exhibits the trait of strong stalk strength. Based on the molecular marker, the stalk strength trait of tartary buckwheat can be selected, and the identification of samples can be completed only by simple DNA extraction, PCR specific amplification and KASP genotyping detection, so that tartary buckwheat with strong stalk strength and tartary buckwheat with poor stalk strength can be distinguished, the tartary buckwheat stalk strength can be quickly screened and identified, the breeding efficiency of tartary buckwheat germplasm with strong stalk strength is improved, a basis is provided for the utilization of excellent allelic variations related to the tartary buckwheat stalk strength trait, and the breeding process is accelerated.