692 results about "Single-nucleotide polymorphism" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to thousand seed weight of wheat and application thereof, and belongs to the technical field of molecular marker breeding. The invention discloses a wheat thousand seed weight related SNP-783 site and application thereof, the SNP site corresponds to the 783rd basic group from the 5'tail end of a sequence shown in SEQ ID NO: 1, and when the site is C / C homozygous, the corresponding genotype is A, and when the site is C / C homozygous, the corresponding genotype is B; when the locus is T / T homozygous, the corresponding genotype is B, and the thousand seed weight of the wheat with the homozygous genotype A is greater than or candidate greater than that of the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to calving interval traits of dairy cows and application of the SNP molecular marker. The number of the SNP molecular markers is four, and the nucleotide sequences of the four markers are as shown in SEQ ID NO.1-4. Researches find that the four SNP molecular markers are significantly related to the Chinese Holstein cattle calving interval traits, and can be used for molecular marker-assisted breeding of the Chinese Holstein cattle calving interval traits to accelerate breeding of Chinese Holstein cattle with strong fecundity. The SNP molecular marker is used for identifying the calving interval character of the Chinese Holstein cattle, the four SNP loci are detected in actual breeding, seed selection and matching are guided, the breeding efficiency is improved, the non-pregnant period is shortened, the production performance of the dairy cattle can be comprehensively improved, the breeding strategy can be optimized, and the population genetic progress can be accelerated.

The invention relates to a product of an SNP (Single Nucleotide Polymorphism) molecular marker related to sow litter size character detection and application of the product. The SNP molecular marker comprises Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt The nucleotide sequence of the gene is shown as SEQ ID No.1, and the nucleotide sequence of the gene is shown as SEQ ID No.1. The product at least comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID No.2, and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The SNP molecular marker provided by the invention can be used for breeding, breeding and evaluating multi-variety sows with high reproductive performance.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention discloses a KASP molecular marker for identifying soybean protein content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82.a2.v1 genome sequence as a reference genome, is the 42148495th SNP on a soybean chromosome 9 and corresponds to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, and when the locus is TT homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, different protein contents of soybeans are screened or screened in an auxiliary mode, and the different protein contents of the soybeans are as follows: the homozygous soybeans of the genotype A are higher than or candidate to be higher than the homozygous soybeans of the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding offspring materials with high protein content.

The invention discloses a biomarker for evaluating litchi quality and a detection method, and relates to the technical field of biology. The marker is an SNP (Single Nucleotide Polymorphism) molecular marker, and the nucleotide sequence of the marker is as shown in SEQ ID NO.1-3. The molecular marker disclosed by the invention can be used for evaluating the quality of litchis, particularly evaluating the fructose content. Meanwhile, when the gene is applied to molecular marker assisted selection (MAS) and genome selection (GS), the genetic improvement progress of the litchi variety can be accelerated.

The invention discloses a KASP molecular marker for identifying the soybean oil content and application, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 6562032th SNP on a soybean 20 # chromosome, and the SNP is the 6562032th SNP on the soybean 20 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is AA homozygous, the corresponding genotype is B; according to the application, soybeans with different oil contents are screened or screened in an auxiliary mode, and the different oil contents of the soybeans are as follows: the soybean with the homozygous genotype A is higher than the soybean with the homozygous genotype B or the soybean with the homozygous genotype B in a candidate mode. The invention has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high oil content.

A molecular marker closely linked to a pepper fruit shape gene. Taking a basic version of Zhangshugang pepper as a reference gene, a single nucleotide polymorphism is present at base 162028839 of the pepper Chr02 chromosome, where a substitution from base C to A occurs. A primer and kit for identifying the molecular marker closely linked to the pepper fruit shape gene, and use thereof in identifying the pepper fruit shape or in molecular-assisted breeding. Early use of the molecular marker, the primer, and the kit in the early stage can quickly screen satisfactory plants, thereby effectively reducing the planting scale, reducing the workload of later identification, and improving the selection efficiency and accuracy. The molecular marker, the primer, and the kit are of great significance in the practice of pepper fruit shape breeding and the research on fruit shape changes and regulation mechanisms.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a single wheat spike number character and application of the SNP molecular marker, and belongs to the technical field of molecular marker breeding. The invention discloses a single wheat spike number related SNP-783 site and application thereof, the SNP site corresponds to the 783rd basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and when the site is C / C homozygous, the corresponding genotype is A, and when the site is C / C homozygous, the corresponding genotype is B, and when the site is C / C homozygous, the corresponding genotype is C / C homozygous; when the locus is T / T homozygous, the corresponding genotype is B, and the ear number of a single plant is as follows: the wheat with the homozygous genotype A is greater than or candidate to be greater than the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, the wheat with the high spike number per plant can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention belongs to the technical field of pig molecular marker-assisted selection, and particularly relates to application of an SNP molecular marker related to the character of the number of live piglets born by pigs. The SNP molecular marker is a basic group of a 32663785th site of a ninth chromosome in a pig genome Sscrofa11.1. 109, the SNP site of the molecular marker has C / T polymorphism, and T is a favorable allelic variation of the character of the number of live piglets born by a pig. The dominant alleles of the SNP are preferably selected, the frequency of the dominant alleles can be increased generation by generation, the number of live piglets of pigs is increased, and the genetic improvement progress of the pigs is accelerated, so that the breeding and production benefits of the pigs are effectively improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to the number of spikelet per ear and the number of grains per ear of wheat and application of the SNP site. The SNP site comprises a kit for identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat, primers and application of the elements in identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat. The invention provides a new method for molecular marker-assisted selective breeding of wheat, and has important significance in cultivation and research of high-yield wheat varieties.

Disclosed are a genomic mating method for Huaxi cattle based on whole genome single nucleotide polymorphism (SNP) information and an application thereof. The method includes the following specific steps: step 1, extracting deoxyribonucleic acid (DNA) from to-be-hybridized Huaxi cattle individuals for genotyping; step 2, performing genotype data imputation to obtain high-density chip data; step 3, calculating an additive genetic relationship matrix, utilizing genomic best linear unbiased prediction (GBLUP) to obtain genomic estimated breeding values of five important economic traits of a to-be-hybridized Huaxi cattle population, and calculating a comprehensive selection index of the individuals; and step 4, using a genetic algorithm to construct a population optimal mating combination list. In the present invention, the breeding cost is greatly saved and an inbreeding level of offspring populations is reduced.

The invention discloses a soybean saline-alkaline tolerance related SNP (Single Nucleotide Polymorphism) site and application thereof, and relates to the technical field of soybean saline-alkaline tolerance gene breeding, the key points of the technical scheme are as follows: the invention provides the SNP site based on GmRfg1 (Glyma. 20G212300), the site can divide a soybean material into E and Q amino acid residue types, and the Q amino acid residue type shows higher saline-alkaline tolerance. The breeding of the Q amino acid residue type (C nucleotide type) soybean variety has important significance for improving the saline-alkaline tolerance of the soybeans and increasing the planting area of the soybeans in saline-alkali soil.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep fecundity and application of the SNP molecular marker. The SNP molecular marker comprises an SNP1 (Single Nucleotide Polymorphism) and / or an SNP2; the SNP1 is located at a base at the 115747587 site of a chromosome 6 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP1 is T / G; the SNP2 is located at a basic group at the 41419566th site of a chromosome 22 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP2 is A / G. According to the scheme, the SNP molecular marker related to sheep fecundity is provided, sheep with the high fecundity character can be efficiently and accurately identified and screened out, and the marker is high in typing quality, single in copy and high in polymorphism and can be used for sheep fecundity molecular marker assisted breeding.

The invention belongs to the technical field of animal molecular markers, and particularly relates to an SNP molecular marker for beef cattle flavor character breeding and application of the SNP molecular marker. The invention proposes that genotype polymorphism of 84181318bp and 84181699bp on chromosome 3 of an international bovine reference genome AR-UCD1.2 version is related to the character of the content of the beef arachidonic acid for the first time, the genotype polymorphism can be used for evaluating the content of the beef arachidonic acid, and beef cattle with specific genotypes at the two sites has relatively high content of the beef arachidonic acid. The site can be used as a molecular marker for evaluating the content of the arachidonic acid in the beef cattle, early prediction and auxiliary screening of beef cattle individuals with excellent meat quality and flavor characters, and a theoretical basis and a technical support are provided for breeding of the beef cattle with high content of the arachidonic acid in the beef cattle.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing the content of porcine inosine and application of the SNP marker. The 10 SNP molecular markers closely related to the porcine inosine content are researched and determined on the basis of American-series purebred landrace, American-series purebred large white pigs and American-series purebred duroc, the dominant allele frequency can be increased generation by generation by optimizing the dominant allele of the SNP, the inosine content can be reduced, and the molecular markers can be used for identifying the porcine inosine content. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to the weight of Beijing fatty chicken and application. The SNP molecular marker has G / A base mutation at 75bp, 655bp and 498bp of the No.4 chromosome of Beijing fatty chicken; the version number of a Beijing fatty chicken reference genome where the SNP molecular marker is located is GRCg6a. The SNP molecular marker provided by the invention can be used as a molecular marker for identifying the weight character of Beijing fatty chicken. The molecular marker provided by the invention is not limited by the age, sex and the like of Beijing fatty chicken, can be used for breeding high-weight dominant Beijing fatty chicken individuals, and greatly accelerates the breeding process of Beijing fatty chicken varieties.

The invention discloses a cucumber 1K liquid phase breeding chip and a special single nucleotide polymorphism variation site thereof. The 1K liquid phase breeding chip provided by the invention comprises a probe combination for detecting 1121 single nucleotide polymorphism variation sites in a cucumber genome, and the position information of a probe corresponding to each single nucleotide polymorphism variation site in a cucumber reference genome is shown in a table 3 in the specification. Single nucleotide polymorphism variation sites involved in the liquid phase breeding chip provided by the invention have the advantages of high polymorphism, good repeatability, stable and reliable marking, convenient statistics and the like, and a DNA fingerprint database for identifying the authenticity of cucumber varieties can be established based on the liquid phase breeding chip. The method can be used for cucumber variety authenticity, early breeding and identification of cucumber varieties and identification of cucumber seed resources, provides technical support for enrichment of cucumber germplasm resources, breeding of new varieties and protection of the new varieties, and has a wide application prospect.

The invention belongs to the field of molecular biological detection, and particularly relates to an FBXO32 gene SNP (Single Nucleotide Polymorphism) marker for rapidly detecting economic traits of Tibetan chicken as well as a detection method and application. It is found for the first time that G / A mutation at the 8916th site of the Tibetan chicken FBXO32 gene is closely related to whether the gene has an Hpa II restriction enzyme cutting site or not, and is closely related to main economic traits of Tibetan chicken. Based on the new discovery and the SNP site, the invention provides a method for rapidly detecting the SNP site and screening economic characters of Tibetan chicken. The method is simple to operate, convenient and fast, low in cost and reliable in result.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker closely linked with the peel thickness of wheat grains and application of the SNP marker, and belongs to the technical field of biology, an SNP site closely linked with the peel thickness character of the wheat grains is excavated through genome-wide association analysis, the SNP site is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat, and the SNP site is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat and is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat. The site base has A / G polymorphism, and the AA genotype or AG genotype has higher grain peel thickness than the GG genotype. Through genotype detection of the SNP site, the wheat grain pericarp thickness character can be obviously distinguished, a KASP primer is developed based on the SNP site, and molecular marker-assisted identification or assisted breeding can be performed on the wheat grain pericarp thickness character.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination for constructing a hazel DNA (Deoxyribose Nucleic Acid) fingerprint spectrum and application thereof. The SNP molecular marker combination comprises 50 SNP molecular markers, can be applied to construction of the hazel plant DNA fingerprint spectrum and further applied to variety identification of hazel plants, fills the blank of the hazel plant SNP molecular markers and the hazel plant DNA fingerprint spectrum based on the SNP molecular markers, and can be used for identifying the hazel plant variety by utilizing DNA fingerprint spectrum information of different hazel varieties. The method can accurately and efficiently distinguish and identify the germplasm resources of the hazel plants, and is of great significance to development and protection of the germplasm resources of the hazel plants.

The invention belongs to the technical field of molecular biology, and particularly discloses a cotton 10K functional site breeding chip and application thereof. The breeding chip is named as' Cotton Core No.1 ', and comprises a 10K functional site targeting capture probe group designed based on a reference genome of disease-resistant, high-quality and high-yield modern upland cotton No.8, the probe group comprises 11159 SNP (Single Nucleotide Polymorphism) loci, 3981 of the SNP loci are functional loci associated with 13 important agronomic characters, and 7178 of the SNP loci are background loci reflecting genetic diversity; the SNP site information is shown in the specification table 1. The invention discloses a cotton 10K functional site breeding chip and application thereof, the breeding chip contains functional sites with more index function genetic variation, has higher detection accuracy among different cotton varieties, is applied to cotton molecular marker-assisted selection breeding, whole genome selection breeding and whole genome correlation analysis, and has higher detection accuracy. The breeding efficiency can be obviously improved, and the practical application is wider.

Compositions, methods, kits, systems, and software are provided for non-invasive prenatal testing for autosomal recessive diseases. Next generation sequencing is used to sequence maternal and fetal DNA isolated from maternal plasma by probe capture. The fetal fraction of the sequencing reads for DNA isolated from maternal plasma is estimated by counting single nucleotide polymorphisms (SNPs) for which an allele is detected that is present in the paternal haplotype but absent in the maternal haplotype, based on the assumption that SNPs having a paternal allele belong to the fetal DNA. The fetal fraction is bioinformatically enriched by excluding sequencing reads over a specified length via in-silico size selection, which increases fetal genotype prediction accuracy. Parental haplotype information together with the read ratios observed at the linked SNPs is used to predict the fetal genotype at a site of a mutation linked to the autosomal recessive disease.

The invention belongs to the field of tumor medicine and the technical field of molecular biology and gene detection, and relates to application of rs17771861 in preparation of a nasopharyngeal carcinoma prognosis prediction marker, and the sequence of the SNP site rs17771861 is as shown in SEQ ID NO.1. The invention also relates to application of the SNP site rs17771861 in preparation of a nasopharyngeal carcinoma prognosis prediction marker. The invention provides an SNP (Single Nucleotide Polymorphism) marker rs17771861 related to nasopharyngeal carcinoma prognosis, application of the SNP marker rs17771861 and a detection kit capable of being used for nasopharyngeal carcinoma prognosis prediction, and the SNP marker rs17771861 is used for assisting in guiding individualized treatment and improving prognosis of tumor patients. After the kit disclosed by the invention is applied to clinical detection, prognosis of a patient can be evaluated before treatment, and a more positive and effective treatment scheme is formulated for the patient with poor prognosis, so that individualized treatment of the patient is realized, and the survival rate is increased. In addition, the kit only needs to detect peripheral blood, and has the characteristics of convenience in sampling, simplicity in operation, high timeliness and the like.

Methods for identifying a modulator of angiogenesis and methods for modulating angiogenesis in a mammalian subject are described herein. In some embodiments, the methods include obtaining data sets from a model for angiogenesis and generating a causal relationship network model based on the obtained data. In some embodiments, the methods include identifying, from the causal relationship network model, a causal relationship unique in angiogenesis, where a gene, lipid, protein, metabolite, transcript, or SNP associated with the unique causal relationship is identified as a modulator of angiogenesis.

A gene chip sensor for single nucleotide polymorphism detection and its application belong to the field of biosensor detection technology. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the existing single nucleotide polymorphism detection methods, the present invention provides a gene chip sensor for single nucleotide polymorphism detection, wherein the gene chip sensor comprises WT wild-type and SNP mutant nucleotide sequences, LSL probe, and CRISPR / Cas 12a system. LSL probe is used as a recognition intermediate to activate the CRISPR / Cas12a system, iron-cobalt nanomaterials are used to provide a stable signal source for sample detection, and screen-printed electrodes are combined with electrochemical sensors; the application of CRISPR / Cas12a and nanomaterial technology in molecular breeding is expanded, and technical support is provided for economical and efficient SNP detection.

The invention belongs to the field of pepper breeding, and discloses a KASP molecular marker closely linked with pepper fruit length, a primer, a kit and application, the molecular marker takes a Ca591.0 version gene as a reference gene, the single nucleotide polymorphism at the 70238018 position of the pepper No.3 chromosome is subjected to base A-T replacement, the flanking nucleotide sequence is shown as SEQ ID No: 1, the nucleotide sequence is shown as SEQ ID No: 2, and the nucleotide sequence is shown as SEQ ID No: 3. The sequences of the primers are shown as SEQ ID No: 2-4, and the kit comprises the primers. The invention also discloses an application of the primer or the kit in identification of different length types of pepper fruits or in molecular assisted breeding of pepper fruit length. A primer and a kit are developed aiming at the molecular marker, and can be used for identifying the length of the pepper fruit, so that assistant breeding is carried out by depending on the molecular marker, and the problems that the conventional breeding period is long and is easily influenced by the environment can be effectively solved.

The invention belongs to the technical field of molecular biology, and discloses an SNP (Single Nucleotide Polymorphism) marker related to the salt tolerance character of micropterus salmoides and application of the SNP marker. According to the invention, correlation analysis is carried out on the extreme salinity tolerance of the micropterus salmoides and the salt tolerance of sensitive individuals, so that the SNP molecular marker related to the salt tolerance of the micropterus salmoides is obtained. The SNP molecular marker is shown as SEQ ID NO: 1, the SNP site is located at the 501st site, and three genotypes of TT, TG and GG exist. Wherein the survival time of the micropterus salmoides with the GG genotype under salinity stress is obviously higher than that of the micropterus salmoides with TG and TT genotypes. By detecting the genotype of the SNP molecular marker, the parent of the micropterus salmoides with strong salt tolerance can be accurately selected, and the SNP molecular marker can be effectively used for molecular marker-assisted breeding of the micropterus salmoides.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.