1532 results about "Single-nucleotide polymorphism" patented technology

A sheep single nucleotide polymorphism (SNP) molecular marker and use thereof in detecting resistance to brucellosis in sheep are provided, relating to the technical field of molecular marker-assisted breeding. A sheep SNP molecular marker is provided, where the sheep SNP molecular marker is associated with resistance to brucellosis in sheep, and has a nucleotide sequence shown in SEQ ID NO: 1 in which a T / C polymorphism is located at 101st bp. The sheep SNP molecular marker can accurately detect a resistance level of sheep to the brucellosis, and is used for the selection of brucellosis-resistant sheep as well as molecular marker-assisted breeding of the resistance to brucellosis in sheep. The sheep SNP molecular marker effectively improves a breeding efficiency of sheep with brucellosis resistance.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the technical field of biology. The SNP molecular markers 1-3 are remarkably related to the growth traits of the litopenaeus vannamei, genomic DNA of muscular tissue of the litopenaeus vannamei to be detected is extracted and then serves as template DNA to be subjected to PCR amplification and sequencing, the genotypes of the three molecular markers are determined, and the litopenaeus vannamei with obvious growth advantages can be rapidly screened out. The molecular marker can accelerate the breeding process of the improved variety of the litopenaeus vannamei, and provides a theoretical basis for mining application of related genetic markers in litopenaeus vannamei molecular marker-assisted breeding.

The invention relates to the technical field of bee breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker and application thereof in improving bee germplasm resources. The SNP molecular marker is based on a genome version PRJNA738447, the SNP molecular marker is located at the 3613559th site of the 11 # chromosome of the bee, and the polymorphism is C / T. The SNP molecular marker provided by the invention is closely linked with the length of the tarsal node of the bee, and the detection of the length of the tarsal node of the bee can be realized through a genotype detection result. The SNP molecular marker provided by the invention can be used for breeding transgenic bees and improving bee germplasm resources, and has important application value in the field of bee breeding.

The invention discloses a litopenaeus vannamei body length growth trait associated SNP molecular marker, a detection primer and application thereof. The sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the SNP site is located at the 282 site from the 5'end of the sequence as shown in SEQ ID NO.1, and the polymorphism of the SNP molecular marker is T / C type. The SNP locus can be used for body length screening of litopenaeus vannamei in litopenaeus vannamei assisted breeding, and a primer designed according to the sequence of SEQ ID NO.1 or a kit prepared from the primer can be used for detecting the SNP locus. The SNP molecular marker Chr3711743678 provided by the invention can be used for assisting in selecting rapid growth parents, and the CC genotype in the SNP molecular marker is used for selecting the parents, so that the growth speed of breeding populations can be increased, an innovative tool is provided for molecular breeding of litopenaeus vannamei, the breeding period is shortened, and industrial development and efficiency improvement are assisted.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker site related to wheat cold resistance and application of the SNP molecular marker site. The molecular site is located at the 101st basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and the polymorphism is C / T. The molecular marker related to wheat cold resistance in the wintering period provided by the invention can be used for detecting whether a wheat variety or strain contains a cold-resistant site or not, and has very high value for cold-resistant breeding of wheat, and meanwhile, the developed KASP molecular marker can be used for greatly accelerating the breeding process of the wheat cold-resistant variety.

The invention provides a specific single nucleotide polymorphism (SNP) molecular marker for sex identification of cobia and application of the specific single nucleotide polymorphism (SNP) molecular marker. Based on whole genome high-throughput sequencing data of male and female cobia, after deep comparative analysis, the specific molecular marker related to the sex of cobia is screened for the first time, and the primer pair designed for the marker can be used independently or used in a combined mode and is high in flexibility. The method has relatively high judgment accuracy, so that the sex of cobia can be quickly and accurately identified, and the method has a very good application prospect.

The invention provides an SNP (Single Nucleotide Polymorphism) site combination for identifying streptococcicosis resistance of tilapia and application of the SNP site combination. DNAs of tilapia tail fin tissues attacked by streptococcus agalactiae are re-sequenced, resistant and sensitive individuals of a tilapia population are screened, GWAS analysis is carried out, SNPs sites related to tilapia streptococcus agalactiae disease resistance are identified, functional gene annotation and enrichment analysis are carried out, two SNP sites LG1320881657 and LG1320548718 are screened, and the SNP sites related to tilapia streptococcus agalactiae disease resistance are obtained. The GG genotype of the LG1320881657 and the TT genotype of the LG1320548718 are the dominant genotypes related to the streptococcus agalactiae disease resistance of the tilapia mossambica. The tilapia mossambica resistant to streptococcosis is successfully screened by utilizing the two SNPs sites, effective SNPs are provided for developing tilapia mossambica anti-streptococcosis molecular marker-assisted breeding, excellent characters of parents are ensured, and the breeding efficiency is improved.

To provide a method for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms in a temperature-dependent fashion.SOLUTION: Provided is a method, in which an invasive oligonucleotide and a primary probe oligo (a 5' flap oligonucleotide) hybridize to a target strand nucleic acid to form an invasive cleavage structure cleavable by a FEN-1 endonuclease to release a cleaved 5' flap (primary cleaved flap); in a secondary reaction, the primary cleaved flap from the primary reaction hybridizes to a FRET cassette 1, a hairpin oligonucleotide labeled with a fluorescent dye and a quencher molecule, to form a second invasive cleavage structure that is cleavable by a FEN-1 endonuclease at a site between the fluorophore and a quencher; and cleavage of FRET cassette 1 separates the quencher from the fluorophore, such that fluorescence signal from the fluorophore can be detected.SELECTED DRAWING: Figure 1

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the testis size character of Hu sheep as well as a screening method and application of the SNP molecular marker, and belongs to the fields of genetic breeding and molecular biology. The marker is located in an intron region of a Hu sheep chromosome 3 LRRIQ1 gene and is remarkably related to the Hu sheep testis size character, and the accuracy and reliability of the SNP molecular marker are ensured through a scientific and rigorous screening method; according to the marker, Hu sheep individuals related to testis size characters can be rapidly and accurately screened out, the breeding efficiency and accuracy are improved, the breeding process is accelerated, the breeding cost is reduced, and the economic benefits are improved; the genetic diversity is protected, the accuracy of seminal fluid quality evaluation is improved, a powerful tool is provided for Hu sheep breeding work, and important basic data is also provided for researching a genetic mechanism of testis development and functions; the method can also be used as an auxiliary diagnosis tool to assist in identifying potential genetic defects or disease risks and promote progress and development of the breeding technology.

The invention relates to a pigeon individual typing detection system, a kit and application thereof. The kit comprises a pigeon individual typing detection system, the pigeon individual typing detection system comprises a primer combination and a composite amplification premix, and the primer combination comprises primers for amplifying 23 STR (short tandem repeat) microsatellite markers, bird sex detection CHD gene loci and 5 SNP (single nucleotide polymorphism) genetic markers. The pigeon individual typing detection system and the kit provided by the invention can realize single-tube detection of 29 pigeon DNA genetic markers, comprise STR genetic markers for realizing pigeon individual identification and parent-child relationship identification and single nucleotide polymorphism genetic markers for evaluating the flying ability of pigeons, and are a detection system which can be detected at present and contains the maximum number of pigeon DNA gene loci; the method has a good application prospect in the fields of pigeon identity identification, parent-child relationship identification and flight ability evaluation.

The invention discloses a rubber tree whole genome SNP molecular marker combination, a gene chip and application, and belongs to the field of molecular marker development. The rubber tree whole genome SNP molecular marker combination comprises 28923 SNP molecular markers, the 28923 SNP molecular markers comprise 28923 SNP sites, each SNP site comprises two different base variation sites and is used for detecting allelic change of the site, and the physical positions of the 28923 SNP sites are determined based on comparison of genome sequences of rubber tree wild germplasm MT / VB / 25A 57 / 8. The 28923 SNP loci adopted by the invention uniformly cover the whole genome of the rubber tree, so that the real level of genetic diversity of the rubber tree can be comprehensively revealed, and the SNP loci can be applied to genetic diversity analysis, population structure analysis, whole-genome association analysis, molecular marker-assisted breeding and whole-genome selective breeding research work of the rubber tree. The whole genome SNP site combination and the gene chip thereof are used for detection, have the advantages of simplicity, rapidness and low cost, and can realize large-scale application in hevea brasiliensis genetic breeding research.

The invention relates to the technical field of artificial intelligence, in particular to a wheat abiotic stress character SNP (Single Nucleotide Polymorphism) prediction method based on an automatic CNN (Convolutional Neural Network) model, which comprises the following steps of: collecting GWAS data related to wheat from a GWAS Atlas database, and constructing a high-quality mutation site data set; performing feature extraction on the data set by using a DNA original sequence, text-based features and structure-based features, sorting the importance of input features by using an SHAP method, assisting feature selection, and explaining the result of the prediction model; then, an automatic architecture search framework is constructed, automatic neural architecture search is adopted, the model is evolved in a specified search space, the optimal performance is achieved on a verification set, and the accuracy, the AUPR and the AUC of the model are evaluated on a test set. The invention provides a deep learning framework which is efficient, accurate and explainable, and is used for predicting the SNP related to the wheat biological stress, so that the development of wheat stress resistance breeding and genomics research is promoted.

The invention relates to a molecular marker associated with pork intramuscular fat and application. The SNP is located at a single nucleotide Ggt occurring at the 6114468bp position of a first chromosome of a pig genome; the A polymorphic site is shown in the specification. A target sequence containing the mononucleotide mutation site is subjected to PCR (Polymerase Chain Reaction) amplification by utilizing a primer, and the PRKN genotype can be quickly identified by sequencing an amplification product, so that the difference of pork quality is detected, and guidance is provided for breeding of pig varieties.

The invention relates to the field of biological detection, in particular to the field of paternity test and detection, and particularly relates to a method for non-invasive prenatal genetic relationship judgment by adopting a two-state SNP (Single Nucleotide Polymorphism) site. According to the method, the minimum number of SNP sites required for judging the genetic relationship is calculated, the genetic relationship is judged by screening the sites containing fetal information in the free DNA sample of the pregnant woman and combining the indexes, the genotype of the pregnant woman sample does not need to be additionally detected, meanwhile, whether the parent-child relationship between a fetus and a suspected parent conforms to the parent-child relationship or not can be accurately judged, and the application range is wider.

The invention discloses a soybean nodulation number related major single nucleotide polymorphism (SNP) site and application thereof, the SNP site is positioned on a soybean chromosome 2, the physical distance of the SNP site in a soybean genome version number Glycine max Wm82.a2.v1 is Chr0243129492, the SNP site corresponds to a 20th basic group of a sequence shown as SEQ ID NO.1, when the site is T / T homozygosis, the corresponding genotype is A, when the site is T / T homozygosis, the corresponding genotype is B, and when the site is T / T homozygosis, the corresponding genotype is C; when the site is C / C homozygous, the corresponding genotype is B; the number of root nodules is as follows: the genotype A homozygous soybean is greater than or candidate to be greater than that of the genotype B homozygous soybean. By detecting the SNP, a tested soybean population can be accurately divided into a low root nodule number (C / C) and a high root nodule number (T / T). Therefore, the molecular marker can be used as a basis for preliminarily identifying the number of soybean nodules, has high efficiency and applicability such as simplicity in operation, low cost and short consumed time, and has important values for digging germplasm of high-nodulation soybeans, solving the problem of nitrogen fertilizer abuse and promoting sustainable development of agriculture.

The invention belongs to the technical field of biological chips, and particularly relates to an erasable and reusable single nucleotide polymorphism gene chip and a detection method, a solid-phase chip substrate loaded with a capture probe, and a closed probe, a primer probe and a fluorescent developing probe which are matched for use, according to the erasable and reusable single nucleotide polymorphism gene chip and the detection method provided by the invention, the gene chip detection technology overcomes the problem that the application cost is high due to the fact that a solid-phase SNP gene detection chip can only be used for one time for a long time, and erasable reuse of the SNP gene detection chip is realized; and the application cost of the solid-phase gene chip for large-scale sample genotyping can be greatly reduced.

The invention provides an SNP (Single Nucleotide Polymorphism) site of an ABO blood group variant, and an A subtype variant blood group identification kit and method, and relates to the technical field of biology. The SNP sites of the ABO blood group variant comprise the 155th c.155Ggt from an initiation codon of an ABO gene coding region, and the 155th c.155Ggt from the initiation codon of the ABO gene coding region; mutation of T. A substance for detecting the SNP site of the ABO blood group variant can be applied to preparation of an A-subtype variant blood group identification method and product so as to be effectively applied to rapid detection of patients and blood donors, the A-subtype variant blood group of an individual can be defined, and possible blood transfusion reaction can be prevented in time.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to thousand seed weight of wheat and application thereof, and belongs to the technical field of molecular marker breeding. The invention discloses a wheat thousand seed weight related SNP-783 site and application thereof, the SNP site corresponds to the 783rd basic group from the 5'tail end of a sequence shown in SEQ ID NO: 1, and when the site is C / C homozygous, the corresponding genotype is A, and when the site is C / C homozygous, the corresponding genotype is B; when the locus is T / T homozygous, the corresponding genotype is B, and the thousand seed weight of the wheat with the homozygous genotype A is greater than or candidate greater than that of the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention belongs to the field of pepper breeding and molecular biology, and discloses a KASP molecular marker closely linked with a pepper fruit color gene CaCHL1, the gene of pepper zunla-1 is used as a reference gene, and the single nucleotide polymorphism at the 13072545th basic group on a pepper chromosome 5 is subjected to basic group G-C replacement. The invention further discloses a primer and a kit for identifying the KASP molecular marker closely linked with the pepper fruit color gene CaCHL1 and application of the primer and the kit in identifying pepper fruit color types or molecular assisted breeding. The screened KASP molecular marker can be directly used for identifying the pepper fruit color, then auxiliary breeding is carried out by depending on the molecular marker, the problems that conventional breeding is long in period and prone to being influenced by the environment can be effectively solved, and the KASP molecular marker has important significance in pepper fruit color breeding practice and research on fruit color change and regulation and control mechanisms.

The invention provides an SNP molecular marker for detecting soybean quality genes and application of the SNP molecular marker, and belongs to the technical field of soybean quality detection. The sequence of the SNP molecular marker is shown as SEQ ID NO.1, an A / T polymorphic site exists at the 71bp in the SEQ ID NO.1, a G / T polymorphic site exists at the 876bp in the SEQ ID NO.1, the 71bp and the 876bp are located at the 6680029th site and the 6680836th site of an encoding gene on a chromosome 18 respectively, the genotypes of the 6680029th site comprise the genotypes of AA, TT and AT, and the genotypes of the 6680836th site comprise the genotypes of GG, TT and GT. The molecular marker identification method disclosed by the invention can be used for rapidly screening out soybean materials with high water-soluble protein content.

The invention belongs to the technical field of animal genetic breeding, and particularly discloses an SNP (Single Nucleotide Polymorphism) chip for distinguishing five kinds of Hubei native cattle (yi-ling cattle, Dabie mountain cattle, troilin cattle, Enshi cattle and delta mountain cattle). Candidate SNP loci are obtained by combining whole genome sequencing and three selection signals (Tajima's D, FST and pi ratio), then the candidate loci are subjected to random forest scoring, the number of the needed SNP loci is further reduced, finally, an SNP locus combination with variety specificity is obtained, probes are designed according to the SNP loci and 60 bp sequences before and after the SNP loci, and the variety specificity is obtained. The SNP chip for distinguishing five kinds of Hubei native cattle can be developed, technical support is provided for variety breeding of Hubei native cattle, and meanwhile, compared with a traditional pedigree and microsatellite variety identification method, the SNP chip has the advantages of being high in accuracy and easy to operate.

The invention discloses a whole-genome low-density chip for breeding high-yield and long-life characters of dairy cows and application of the whole-genome low-density chip. The invention firstly discloses a whole-genome low-density chip for breeding the high-yield and long-life character of the dairy cow, the whole-genome low-density chip comprises 10000 SNP (Single Nucleotide Polymorphism) loci, ARS-UCD1.2 is used as a reference genome, and the information of the 10000 SNP loci is shown in a table 6. The invention further discloses application of the whole-genome low-density chip. The 10,000 SNP loci contained in the whole genome low-density chip are uniformly distributed on chromosomes, cover the whole genome and important character loci, can effectively improve the longevity and the accuracy of milk yield breeding, and can be applied to gene screening, gene positioning, marker-assisted breeding and the like. In addition, the detection cost of the low-density chip is greatly reduced, and the low-density chip is more suitable for commercialized dairy farms and has important significance on dairy cow breeding and dairy cow group improvement.

The invention belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to growth traits of grass carp and application of the SNP molecular marker. The SNP molecular marker is located at the position of 23608308bp of the eleventh chromosome (SLG1123608308) of the grass carp, and is Tgt; and A basic group mutation. Experiments show that the SNP molecular marker developed by the invention has significant influence on four growth traits (Plt; 0.05), and the allele A has more excellent growth traits. The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker, an amplification primer or a kit in breeding grass carp with excellent growth traits. The SNP molecular marker provided by the invention is not limited by the age, sex and the like of the grass carp, can be used for breeding grass carp varieties with excellent growth traits, and even can be accurately screened after being hatched, so that the breeding process of the grass carp varieties with excellent growth traits is greatly accelerated.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the field of molecular marker-assisted breeding of aquatic animals. The genome DNA of the muscular tissue of the litopenaeus vannamei to be detected is extracted as a template, the gene is subjected to PCR amplification and sequencing, the genotype of the molecular marker SNP1-3 is determined, and the molecular marker SNP1-3 is used as the SNP molecular marker for seed selection, so that the aim of quickly screening litopenaeus vannamei individuals with obvious growth advantages in the early stage is fulfilled. According to the molecular marker related to the growth traits of the litopenaeus vannamei, the breeding period can be greatly shortened, the fine breed breeding process of the litopenaeus vannamei is accelerated, the breeding efficiency is improved, and the breeding cost is reduced.

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the daily gain character of a pig. The SNP molecular marker is a Ggt (Glutathione Ggt) located at the position of 160773437bp of a first chromosome of a pig genome Ensembl Sscrofa11.1 version; a mutation. Through the SNP molecular marker, the SNP molecular marker can be applied to breeding of the daily weight gain character of the Duroc long commercial pigs, so that the daily weight gain character of the Duroc long commercial pigs is improved, the growth performance and the slaughter weight of the Duroc long commercial pigs are improved, and the economic value and the economic benefit of the Duroc long commercial pigs are improved. And the variety of the Duroc long commercial pigs can be improved, the daily gain character of the variety is further improved, improved variety breeding is performed, and the market competitiveness of the Duroc long commercial pigs is improved. In addition, due to the fact that the Duroc long hybrid population is adopted as a research object, the Duroc long hybrid population can also serve as a reference population of a male parent Duroc purebred population, and reference and application values are improved for breeding of male parent Duroc boars in daily gain.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to calving interval traits of dairy cows and application of the SNP molecular marker. The number of the SNP molecular markers is four, and the nucleotide sequences of the four markers are as shown in SEQ ID NO.1-4. Researches find that the four SNP molecular markers are significantly related to the Chinese Holstein cattle calving interval traits, and can be used for molecular marker-assisted breeding of the Chinese Holstein cattle calving interval traits to accelerate breeding of Chinese Holstein cattle with strong fecundity. The SNP molecular marker is used for identifying the calving interval character of the Chinese Holstein cattle, the four SNP loci are detected in actual breeding, seed selection and matching are guided, the breeding efficiency is improved, the non-pregnant period is shortened, the production performance of the dairy cattle can be comprehensively improved, the breeding strategy can be optimized, and the population genetic progress can be accelerated.

The invention provides application of a Zm00001eb378820 gene in molecular marker-assisted breeding for regulating and controlling the hundred-grain weight of corn grains, and relates to the technical field of agricultural biology. A backbone maize inbred line Ye107 is used as a male parent and hybridized with three excellent inbred lines (D39, R-2-1-1 and YML1218), a multi-parent group with remarkable hundred-grain weight difference is constructed, and SNP27712538 positioned on a chromosome 9 is a remarkable associated site by measuring hundred-grain weight phenotype data in multiple environments and combining high-density SNP (Single Nucleotide Polymorphism) markers, GWAS analysis and QTL (Quantitative Trait Loci) positioning. And the functional gene Zm00001eb378820 which is closely related to the regulation and control of the corn hundred-grain weight is excavated. A new insight is provided for a genetic regulation mechanism of the corn hundred-grain weight, and a key target spot is provided for breeding of high-yield corn varieties, so that the breeding efficiency is remarkably improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker linked with a major QTL (Quantitative Trait Loci) related to grass carp body weight traits and application of the SNP molecular marker, the SNP molecular marker is positioned on a 13428777 site basic group of a 24th chromosome of grass carp, and polymorphism of two alleles A and G appears at the site. The screened SNP molecular marker is closely related to the main effect QTL locus of the grass carp body weight character, and plays an important role in regulation and control of the grass carp body weight. When the genotype of the SNP molecular marker is AA, the average body weight and average body length of the grass carp are higher than those of individuals with genotypes of AG and GG. Therefore, the SNP molecular marker can be used for assisting in selection of grass carp breeding, and especially has a wide application prospect when being used for selecting grass carp strains with high growth speed.

The invention discloses an SNP (Single Nucleotide Polymorphism) site and a KASP primer group for identifying or assisting in identifying clematis maple and application of the SNP site and the KASP primer group. The SNP site is located at the 2688th site of SEQ ID No.1 (sequence identifier number 1). The KASP primer group is composed of single-stranded DNA (deoxyribonucleic acid) as shown in SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The method comprises the following steps: carrying out next-generation sequencing and assembling on clematis maple for the first time, taking formed ultra-long scaffold as a reference genome ultra-long fragment, carrying out simplified genome sequencing on 15 clematis plants, and comparing the simplified genome sequencing with the reference genome ultra-long fragment to obtain difference sites, finally, the KASP molecular marker CA24547 is developed on the basis of the difference sites, and the KASP molecular marker CA24547 is verified. The KASP molecular marker developed by the invention can realize accurate identification of the clematis nepalensis in the seedling stage, and has important significance for protecting the clematis nepalensis.