515 results about "Single-nucleotide polymorphism" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to thousand seed weight of wheat and application thereof, and belongs to the technical field of molecular marker breeding. The invention discloses a wheat thousand seed weight related SNP-783 site and application thereof, the SNP site corresponds to the 783rd basic group from the 5'tail end of a sequence shown in SEQ ID NO: 1, and when the site is C / C homozygous, the corresponding genotype is A, and when the site is C / C homozygous, the corresponding genotype is B; when the locus is T / T homozygous, the corresponding genotype is B, and the thousand seed weight of the wheat with the homozygous genotype A is greater than or candidate greater than that of the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to calving interval traits of dairy cows and application of the SNP molecular marker. The number of the SNP molecular markers is four, and the nucleotide sequences of the four markers are as shown in SEQ ID NO.1-4. Researches find that the four SNP molecular markers are significantly related to the Chinese Holstein cattle calving interval traits, and can be used for molecular marker-assisted breeding of the Chinese Holstein cattle calving interval traits to accelerate breeding of Chinese Holstein cattle with strong fecundity. The SNP molecular marker is used for identifying the calving interval character of the Chinese Holstein cattle, the four SNP loci are detected in actual breeding, seed selection and matching are guided, the breeding efficiency is improved, the non-pregnant period is shortened, the production performance of the dairy cattle can be comprehensively improved, the breeding strategy can be optimized, and the population genetic progress can be accelerated.

The invention relates to a product of an SNP (Single Nucleotide Polymorphism) molecular marker related to sow litter size character detection and application of the product. The SNP molecular marker comprises Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt, Cgt The nucleotide sequence of the gene is shown as SEQ ID No.1, and the nucleotide sequence of the gene is shown as SEQ ID No.1. The product at least comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID No.2, and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The SNP molecular marker provided by the invention can be used for breeding, breeding and evaluating multi-variety sows with high reproductive performance.

The invention belongs to the field of SNP (Single Nucleotide Polymorphism) detection, and particularly provides an SNP molecular marker related to a sheep body length character, application of the SNP molecular marker and a kit for detecting the SNP molecular marker. The kit provided by the invention is used for performing polymorphism detection on the SNP molecular genetic marker, namely the 92690768th site of the No.4 chromosome, screening and determining the sheep body length character, and selecting the body length character to breed or assist to breed a long sheep individual with the body length character, so that a Tibetan sheep variety germplasm resource with high-quality body length character can be collected, and the kit has a very good application prospect.

The invention discloses a biomarker for evaluating litchi quality and a detection method, and relates to the technical field of biology. The marker is an SNP (Single Nucleotide Polymorphism) molecular marker, and the nucleotide sequence of the marker is as shown in SEQ ID NO.1-3. The molecular marker disclosed by the invention can be used for evaluating the quality of litchis, particularly evaluating the fructose content. Meanwhile, when the gene is applied to molecular marker assisted selection (MAS) and genome selection (GS), the genetic improvement progress of the litchi variety can be accelerated.

A molecular marker closely linked to a pepper fruit shape gene. Taking a basic version of Zhangshugang pepper as a reference gene, a single nucleotide polymorphism is present at base 162028839 of the pepper Chr02 chromosome, where a substitution from base C to A occurs. A primer and kit for identifying the molecular marker closely linked to the pepper fruit shape gene, and use thereof in identifying the pepper fruit shape or in molecular-assisted breeding. Early use of the molecular marker, the primer, and the kit in the early stage can quickly screen satisfactory plants, thereby effectively reducing the planting scale, reducing the workload of later identification, and improving the selection efficiency and accuracy. The molecular marker, the primer, and the kit are of great significance in the practice of pepper fruit shape breeding and the research on fruit shape changes and regulation mechanisms.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a single wheat spike number character and application of the SNP molecular marker, and belongs to the technical field of molecular marker breeding. The invention discloses a single wheat spike number related SNP-783 site and application thereof, the SNP site corresponds to the 783rd basic group from the 5'tail end of a sequence as shown in SEQ ID NO.1, and when the site is C / C homozygous, the corresponding genotype is A, and when the site is C / C homozygous, the corresponding genotype is B, and when the site is C / C homozygous, the corresponding genotype is C / C homozygous; when the locus is T / T homozygous, the corresponding genotype is B, and the ear number of a single plant is as follows: the wheat with the homozygous genotype A is greater than or candidate to be greater than the wheat with the homozygous genotype B. The SNP has high effectiveness and potential application value, the wheat with the high spike number per plant can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention belongs to the technical field of pig molecular marker-assisted selection, and particularly relates to application of an SNP molecular marker related to the character of the number of live piglets born by pigs. The SNP molecular marker is a basic group of a 32663785th site of a ninth chromosome in a pig genome Sscrofa11.1. 109, the SNP site of the molecular marker has C / T polymorphism, and T is a favorable allelic variation of the character of the number of live piglets born by a pig. The dominant alleles of the SNP are preferably selected, the frequency of the dominant alleles can be increased generation by generation, the number of live piglets of pigs is increased, and the genetic improvement progress of the pigs is accelerated, so that the breeding and production benefits of the pigs are effectively improved.

The invention discloses a soybean saline-alkaline tolerance related SNP (Single Nucleotide Polymorphism) site and application thereof, and relates to the technical field of soybean saline-alkaline tolerance gene breeding, the key points of the technical scheme are as follows: the invention provides the SNP site based on GmRfg1 (Glyma. 20G212300), the site can divide a soybean material into E and Q amino acid residue types, and the Q amino acid residue type shows higher saline-alkaline tolerance. The breeding of the Q amino acid residue type (C nucleotide type) soybean variety has important significance for improving the saline-alkaline tolerance of the soybeans and increasing the planting area of the soybeans in saline-alkali soil.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep fecundity and application of the SNP molecular marker. The SNP molecular marker comprises an SNP1 (Single Nucleotide Polymorphism) and / or an SNP2; the SNP1 is located at a base at the 115747587 site of a chromosome 6 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP1 is T / G; the SNP2 is located at a basic group at the 41419566th site of a chromosome 22 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP2 is A / G. According to the scheme, the SNP molecular marker related to sheep fecundity is provided, sheep with the high fecundity character can be efficiently and accurately identified and screened out, and the marker is high in typing quality, single in copy and high in polymorphism and can be used for sheep fecundity molecular marker assisted breeding.

The invention belongs to the technical field of animal molecular markers, and particularly relates to an SNP molecular marker for beef cattle flavor character breeding and application of the SNP molecular marker. The invention proposes that genotype polymorphism of 84181318bp and 84181699bp on chromosome 3 of an international bovine reference genome AR-UCD1.2 version is related to the character of the content of the beef arachidonic acid for the first time, the genotype polymorphism can be used for evaluating the content of the beef arachidonic acid, and beef cattle with specific genotypes at the two sites has relatively high content of the beef arachidonic acid. The site can be used as a molecular marker for evaluating the content of the arachidonic acid in the beef cattle, early prediction and auxiliary screening of beef cattle individuals with excellent meat quality and flavor characters, and a theoretical basis and a technical support are provided for breeding of the beef cattle with high content of the arachidonic acid in the beef cattle.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing the content of porcine inosine and application of the SNP marker. The 10 SNP molecular markers closely related to the porcine inosine content are researched and determined on the basis of American-series purebred landrace, American-series purebred large white pigs and American-series purebred duroc, the dominant allele frequency can be increased generation by generation by optimizing the dominant allele of the SNP, the inosine content can be reduced, and the molecular markers can be used for identifying the porcine inosine content. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to the weight of Beijing fatty chicken and application. The SNP molecular marker has G / A base mutation at 75bp, 655bp and 498bp of the No.4 chromosome of Beijing fatty chicken; the version number of a Beijing fatty chicken reference genome where the SNP molecular marker is located is GRCg6a. The SNP molecular marker provided by the invention can be used as a molecular marker for identifying the weight character of Beijing fatty chicken. The molecular marker provided by the invention is not limited by the age, sex and the like of Beijing fatty chicken, can be used for breeding high-weight dominant Beijing fatty chicken individuals, and greatly accelerates the breeding process of Beijing fatty chicken varieties.

The invention belongs to the field of molecular biological detection, and particularly relates to an FBXO32 gene SNP (Single Nucleotide Polymorphism) marker for rapidly detecting economic traits of Tibetan chicken as well as a detection method and application. It is found for the first time that G / A mutation at the 8916th site of the Tibetan chicken FBXO32 gene is closely related to whether the gene has an Hpa II restriction enzyme cutting site or not, and is closely related to main economic traits of Tibetan chicken. Based on the new discovery and the SNP site, the invention provides a method for rapidly detecting the SNP site and screening economic characters of Tibetan chicken. The method is simple to operate, convenient and fast, low in cost and reliable in result.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker closely linked with the peel thickness of wheat grains and application of the SNP marker, and belongs to the technical field of biology, an SNP site closely linked with the peel thickness character of the wheat grains is excavated through genome-wide association analysis, the SNP site is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat, and the SNP site is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat and is located at the 672th, 214th and 121th positions of the 2A chromosome of the wheat. The site base has A / G polymorphism, and the AA genotype or AG genotype has higher grain peel thickness than the GG genotype. Through genotype detection of the SNP site, the wheat grain pericarp thickness character can be obviously distinguished, a KASP primer is developed based on the SNP site, and molecular marker-assisted identification or assisted breeding can be performed on the wheat grain pericarp thickness character.

Compositions, methods, kits, systems, and software are provided for non-invasive prenatal testing for autosomal recessive diseases. Next generation sequencing is used to sequence maternal and fetal DNA isolated from maternal plasma by probe capture. The fetal fraction of the sequencing reads for DNA isolated from maternal plasma is estimated by counting single nucleotide polymorphisms (SNPs) for which an allele is detected that is present in the paternal haplotype but absent in the maternal haplotype, based on the assumption that SNPs having a paternal allele belong to the fetal DNA. The fetal fraction is bioinformatically enriched by excluding sequencing reads over a specified length via in-silico size selection, which increases fetal genotype prediction accuracy. Parental haplotype information together with the read ratios observed at the linked SNPs is used to predict the fetal genotype at a site of a mutation linked to the autosomal recessive disease.

The invention belongs to the field of pepper breeding, and discloses a KASP molecular marker closely linked with pepper fruit length, a primer, a kit and application, the molecular marker takes a Ca591.0 version gene as a reference gene, the single nucleotide polymorphism at the 70238018 position of the pepper No.3 chromosome is subjected to base A-T replacement, the flanking nucleotide sequence is shown as SEQ ID No: 1, the nucleotide sequence is shown as SEQ ID No: 2, and the nucleotide sequence is shown as SEQ ID No: 3. The sequences of the primers are shown as SEQ ID No: 2-4, and the kit comprises the primers. The invention also discloses an application of the primer or the kit in identification of different length types of pepper fruits or in molecular assisted breeding of pepper fruit length. A primer and a kit are developed aiming at the molecular marker, and can be used for identifying the length of the pepper fruit, so that assistant breeding is carried out by depending on the molecular marker, and the problems that the conventional breeding period is long and is easily influenced by the environment can be effectively solved.

The invention belongs to the technical field of molecular biology, and discloses an SNP (Single Nucleotide Polymorphism) marker related to the salt tolerance character of micropterus salmoides and application of the SNP marker. According to the invention, correlation analysis is carried out on the extreme salinity tolerance of the micropterus salmoides and the salt tolerance of sensitive individuals, so that the SNP molecular marker related to the salt tolerance of the micropterus salmoides is obtained. The SNP molecular marker is shown as SEQ ID NO: 1, the SNP site is located at the 501st site, and three genotypes of TT, TG and GG exist. Wherein the survival time of the micropterus salmoides with the GG genotype under salinity stress is obviously higher than that of the micropterus salmoides with TG and TT genotypes. By detecting the genotype of the SNP molecular marker, the parent of the micropterus salmoides with strong salt tolerance can be accurately selected, and the SNP molecular marker can be effectively used for molecular marker-assisted breeding of the micropterus salmoides.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

The invention belongs to the field of molecular biological detection, and particularly relates to a PLS3 gene SNP marker for rapidly detecting economic traits of Tibetan chicken, a detection method and application. It is found for the first time that G / A mutation at the 28469th site of the Tibetan chicken PLS3 gene is closely related to whether the gene has a BstZ17 I restriction enzyme cutting site or not, and is closely related to main economic traits of Tibetan chicken. Based on the new discovery and the SNP site, the invention provides a method for rapidly detecting the SNP site and screening economic characters of Tibetan chicken. The method is simple to operate, convenient and fast, low in cost and reliable in result.

The invention discloses an SNP molecular marker for increasing the milk yield of Holstein cattle and application of the SNP molecular marker, the SNP molecular marker is located at a mutation site of a Holstein cattle genome Leptin gene, the gene number of the Leptin gene in an NCBI database is NC037333.1, the SNP molecular marker is located at the 1761th basic group of a cDNA sequence of the Leptin gene, and the mutation basic group is A or G. When the basic group of the SNP molecular marker is A, the genotype is AA or AG, and the milk fat rate and the milk protein rate of Holstein cow milk production are high; when the basic group of the SNP molecular marker is G, the genotype is GG, the milk yield of Holstein cattle in 305 days is high, and the milk quality is not obviously reduced. The marker is applied to genetic improvement of dairy cows, and the frequency of dominant alleles can be improved generation by generation by selecting individuals carrying the dominant alleles, so that the lactation performance of the dairy cows is effectively improved, the breeding efficiency is optimized, and the competitive advantage in the field of dairy cow breeding is enhanced.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to pork quality traits. Basic groups at 208bp and 217bp from the 5'end of the sequence have synchronous C / T mutation, the sequence shows three genotypes of CC, CT or TT, T allele is dominant allele, and the intramuscular fat content of the TT type pig is greater than that of the CC type pig. The invention further develops a reagent for detecting the SNP molecular marker, the reagent can be used for pig assisted breeding, pig breeding, pork quality character identification or pork quality character screening, and a method for identifying the intramuscular fat content of pork and screening pork with rich intramuscular fat is developed.

The invention belongs to the field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker combination for genetic relationship identification of wuzhu cattle and application of the SNP molecular marker combination. The SNP marker combination is composed of 140 high-polymorphism SNP sites distributed on a plurality of chromosomes of a bovine whole genome, the average distance between the sites is reasonable, and the SNP marker combination has good genome coverage. The selected SNP site has high allele frequency (MAF), expected heterozygosity (He) and polymorphic information content (PIC), and the cumulative exclusion probability is 0.9999. King and Plink software are combined to deduce a genetic relationship coefficient (Kinship) and an IBD shared value (PIHAT) between samples, and a result shows that the marker combination can realize a genetic relationship recognition effect equivalent to that of whole genome SNP data in Wuzhu white cattle (grassland white cattle), and the scientificity and practicability of the marker combination are verified. The SNP molecular marker combination can be widely applied to the processes of germplasm resource management, pedigree correction and cattle breeding, and has good popularization prospects and economic benefits.

The invention relates to the technical field of biological information, and provides a whole genome selection method, device and equipment of a graph neural network and a storage medium, and the whole genome selection method of the graph neural network comprises the following steps: mapping single nucleotide polymorphism (SNP) to a gene level, and converting the SNP into a gene embedding vector; constructing a gene interaction network based on preset multi-source biological priori knowledge; inputting the target character into a graph neural network model to obtain a predicted value of the target character output by the graph neural network model; wherein the graph neural network model is determined based on a gene embedding vector and a gene interaction network. According to the method, the SNP is mapped to the gene level, and the gene interaction network is combined, so that the prediction result has clear biological significance; and the gene interaction network is constructed based on the preset multi-source biological priori knowledge, so that interaction information between genes can be fully utilized, and the accuracy of target character prediction is improved.

The invention specifically discloses three SNP (Single Nucleotide Polymorphism) molecular markers obviously related to Chinese Holstein cattle somatic cell scoring (SCS) and application thereof. The SNP is located in the following aspects: 1) in a No.8 chromosome 15818198bp (LINGO2 gene, Ggt; t), genotype effect GGlt; gTlt; GTlt; tT; 2), in the 17th chromosome 16535314bp (Ggt in the ZNF330 gene; a), effect GGlt; aA1t; AA1t; gA; 3) in the 69672126bp (in the PACS2 gene, Ggt; a), effect GGlt; gAlt; aA. SCS is an index of mastitis, and high SCS corresponds to high morbidity rate of mastitis. By detecting the genotypes of the sites, individuals with low SCS (namely, low mastitis morbidity) can be accurately bred in the early stage, the disease-resistant breeding efficiency and accuracy are remarkably improved, the reduction of the mastitis morbidity of groups is accelerated, and the production performance and economic benefits are improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker positioned on a No.15 chromosome of a sow and related to the lactation ability of the sow. The SNP site of the SNP molecular marker corresponds to the Agt at the 31884611bp position on the No.15 chromosome of an international pig reference genome version 11.1; g mutation; the genotype of the gene is AA, AG or GG. The SNP molecular marker provided by the invention is remarkably related to the sow lactation ability character, the identification of the sow lactation ability character can be realized by identifying the single nucleotide polymorphism of the SNP molecular marker and / or the genotype of the SNP molecular marker, and the dominant allele frequency can be increased generation by generation and the sow lactation ability can be improved by preferably selecting the dominant allele G of the SNP provided by the invention. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention relates to the technical field of molecular detection, discloses a KASP molecular marker related to a sheep backfat thickness character, a detection primer group and application of the KASP molecular marker and the detection primer group, and aims to provide a KASP molecular marker for detecting a sheep Chr15: g.12672188 site Tgt; the invention discloses a specific primer group for C variation, which consists of the following three primers: a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 1, and a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 2, the nucleotide sequence of the locus C allele typing primer VIC is as shown in SEQ ID NO: 2. The invention discovers and discloses Tgt of Chr15: g.12672188 site in a sheep genome for the first time; the C single nucleotide polymorphism (SNP) is obviously associated with the backfat thickness character. Association analysis of 118 Auodurau lake rams proves that the back fat thickness of CC and TT genotype individuals is obviously higher than that of TC genotype individuals. The discovery reveals that the two homozygous genotypes of the site are unique genetic patterns of dominant genotypes, and a direct and reliable molecular target is provided for genetic improvement of the sheep backfat thickness.

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to the risk of liver injury caused by methotrexate and application of the SNP site. The SNP is located at 31, 356 and 966bp positions of a No.6 chromosome of a human GRCh38 genome, and the basic group of the SNP is C or G. According to the scheme, the SNP site related to the methotrexate liver injury is provided, the risk of the liver injury caused by methotrexate can be effectively predicted, and the SNP site serves as a marker for predicting the liver injury caused by methotrexate.

The invention relates to an SNP molecular marker related to litchi fruit weight and application thereof, and belongs to the technical field of molecular biology. The SNP molecular marker related to the litchi fruit weight is based on a seedless litchi reference genome, the SNP molecular marker is located in a litchi SL05G0138630 gene coding region, and is located at the 27564818th basic group of a litchi chromosome 5, and the basic group is A or G. The application finds that a non-synonymous SNP site in a coding region of the litchi SL05G0138630 gene is closely associated with the litchi fruit weight phenotype, the variety with the weight of the litchi fruit can be efficiently identified by detecting the polymorphic site, the invention further discloses an identification method for the molecular level of the variety with the weight of the litchi fruit, and the molecular level of the variety with the weight of the litchi fruit can be identified by an SNP-based typing method. And cheap and accurate gene SNP typing can be achieved, so that the purpose of efficiently and accurately identifying the weight variety of the litchi fruit is achieved.