67 results about "Gene expression level" patented technology

This invention provides a method and apparatus for splitting single-cell mixed sample sequencing data, relating to the field of biotechnology. The splitting method includes: capturing and sequencing single-cell suspensions using a single-cell platform; then performing reference genome alignment, cell identification, and gene expression level quantification on the sequencing data using Cellranger; splitting the cell data identified in step a into two groups of cell data for different sexes based on SNP locus information from the 1000 Genomes Project; and distinguishing the two groups of cell data from male or female samples based on the proportion of sex-specific genes expressed in the two groups of cell data. This splitting method eliminates the need for additional experimental operations such as protein labeling and genome sequencing, and can provide accurate and reliable data splitting even when individual SNP information is unavailable.

The application relates to the technical field of biological medicine, and discloses application of a reagent for detecting CBS gene expression level in preparation of a breast cancer diagnosis and / or prognosis product. The nucleotide sequence of the CBS gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded CBS protein is shown as SEQ ID NO. 2. The experimental results of the application show that the expression of the CBS protein in breast cancer tissues is significantly higher than that in paracancerous tissues, and the CBS protein can be used as a biomarker for breast cancer diagnosis. Meanwhile, the ROC curve evaluation of the CBS protein expression amount on the prognosis results of breast cancer patients shows that the AUC value reaches 0.841, which also shows that the CBS protein can be used as a marker for the prognosis of breast cancer patients, and the CBS protein has important diagnostic significance for the prognosis of breast cancer.

The present application belongs to the field of biomedical technology, and particularly relates to an axial length and growth trend evaluation method of eye axis based on PDGFRA gene and a PDGFRA enzyme activity control agent. The method comprises the following steps: 1) collecting an eye biological sample of a subject; 2) based on the expression level of PDGFRA gene of platelet-derived growth factor receptor alpha in the collected biological sample; 3) based on the expression level, calculating the axial length of the eye axis according to the quantitative relationship between the expression amount of PDGFRA and the axial length of the eye axis, and predicting the growth trend of the axial length of the eye axis through additional calculation. The technical scheme of the present application can effectively detect and evaluate the axial length of the eye axis, effectively judge the growth trend of the axial length of the eye axis, and directly and efficiently control the axial length of the eye axis and its growth trend through the existing drug components.

This invention belongs to the field of molecular breeding technology and provides a method based on... YIPF1 Molecular breeding methods and applications for inhibiting egg production decline in laying hens using the eQTL gene. This invention combines molecular markers with... YIPF1 Gene expression levels have a regulatory role; the combined molecular markers are located at Chr8:24801464 and Chr8:24802450 loci in the chicken genome, respectively. This invention breaks through the traditional single-trait research paradigm, integrating multidimensional omics and phenotypic data to identify genes from a systems biology perspective. YIPF1 The gene and its regulatory site combination, Chr8:24801464 and Chr8:24802450, serve as key targets in the process of tissue functional decline in laying hens. This gene possesses cross-tissue, dynamically correlated early warning characteristics, providing a reliable tool for early assessment and precise breeding of anti-aging traits in laying hens, and has significant application value for achieving the goal of an ultra-long laying cycle.

This invention belongs to the field of agricultural biotechnology, specifically relating to a type of sisal. DREB Genes and their application in improving plant cold resistance. This invention provides sisal. DREB The gene expression level increased significantly after low-temperature stress, indicating a close relationship between its expression and the response of sisal to low-temperature stress. The study found that transgenic methods can significantly improve the expression level of sisal. DREB Genes were overexpressed, along with sisal DREB Increased gene expression levels led to improved cold resistance in transgenic Arabidopsis thaliana. Overexpression of sisal in plants also improved cold resistance. DREB The discovery that genes can enhance plant cold resistance can be used to breed new varieties or seedlings of cold-resistant plants, providing a theoretical basis and genetic resources for genetic breeding, which is of great significance to agricultural production.

The application provides a KRT31 gene knockout cell line and its application in promoting replication of viruses of the Picornaviridae family and / or production of vaccines of viruses of the Picornaviridae family, and belongs to the technical field of genetic engineering. The application provides an application of a KRT31 gene or a coded protein thereof as a target in preparation of a product for regulating replication of viruses of the Picornaviridae family and / or vaccine production. Up-regulation of the expression level of the KRT31 gene can inhibit replication of viruses of the Picornaviridae family, and down-regulation of the expression level of the KRT31 gene can promote replication of viruses of the Picornaviridae family. The application adopts sgRNA to knockout the KRT31 gene to prepare a cell line with lost function of a gene coded protein, so as to promote replication of viruses of the Picornaviridae family, improve virus titer and antigen yield, and help to efficiently prepare vaccines of viruses of the Picornaviridae family.

This invention belongs to the field of biomedicine and relates to the application of a detection reagent for a marker of mixed connective tissue diseases. This invention discloses... MEF2C Genes can serve as biomarkers for mixed connective tissue disease; they are found in the peripheral blood of patients with mixed connective tissue disease. MEF2C Gene expression levels were significantly higher in the group than in the healthy group. This invention provides a detection reagent for a mixed connective tissue disease marker, comprising an upstream primer with the nucleotide sequence shown in SEQ ID NO.3 and a downstream primer with the nucleotide sequence shown in SEQ ID NO.4, which can detect... MEF2C Gene expression levels, which can then be used to develop products for the early diagnosis of mixed connective tissue diseases.

This invention discloses the application of LNA1 protein and related biological materials in regulating the number of leaves on the ear and yield of maize. The invention first involves overexpression of LNA1 protein in wild-type maize. lna1 , received lna1 Gene-modified maize and statistical analysis of its leaf number revealed that overexpression... lna1 It can increase the number of leaves on the corn ear and the total number of leaves. To further verify the function of LNA1, this invention knocked out LNA1 in wild-type corn. lna1, get lna1 Knockout corn and statistical analysis of its leaf number and plant height revealed that: lna1 It can reduce the number of leaves on the corn ear and the plant height. Furthermore, this invention has demonstrated improved efficiency by constructing NIL-Popcorn and NIL-MS71. lna1 Gene expression levels can increase maize yield. These results indicate that the LNA1 protein and related biological materials can regulate leaf number, yield, and plant height, and will play an important role in breeding high-yielding maize varieties.

This invention belongs to the field of biological control technology for brown planthoppers, specifically relating to the application of the Tip60 gene and its encoded protein in the control of brown planthoppers. This invention is the first to discover that the Tip60 gene plays a crucial role in the control of brown planthoppers. Further experiments confirmed that reducing the expression level of the Tip60 gene significantly shortens the survival time of female brown planthoppers and reduces the number of offspring, indicating that inhibiting Tip60 gene expression can effectively control brown planthoppers, providing a theoretical basis for this strategy. Simultaneously, this invention uses RNAi technology to interfere with the expression of this gene during the adult stage of brown planthoppers, producing a significant inhibitory effect, thus providing a novel strategy and target for brown planthopper control, and demonstrating the application prospects of RNAi technology in silencing the Tip60 gene in brown planthopper control.

This invention belongs to the field of biotechnology and relates to a... ZmCASPL11 The application of genes in improving plant heat tolerance. ZmCASPL11 The gene encodes a protein belonging to the Casparian band structure family of membrane proteins (CASPL), whose expression level is significantly upregulated under high temperature stress. Mutants of this gene... ZmCASPL11 It exhibits a high-temperature sensitive phenotype. The present invention describes... ZmCASPL11 Genes were transformed into Arabidopsis thaliana and their function was validated. Compared with wild-type Arabidopsis thaliana, overexpression of [the gene] showed [significant improvement]. ZmCASPL11 The gene significantly enhances the tolerance of Arabidopsis thaliana plants to heat stress. This invention provides more theoretical basis for research on heat resistance in maize, and offers genetic resources and biotechnological means for the subsequent breeding of new heat-resistant maize lines and the innovation of germplasm resources.

This application belongs to the field of microbial technology, and mainly relates to a strain of *Pediococcus lactis* BPA03 and its applications. *Pediococcus lactis* BPA03 is classified and named *Pediococcus lactis*. Pediococcus acidilactici This *Pediococcus lactis* strain BPA03 was deposited on September 28, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36109. It inhibits skin melanin deposition and improves UV photoaging, suppresses the gene expression levels of immune factors such as IL-1β, IL-6, IL-8, and TNF-α, improves the activities of superoxide dismutase, catalase, and glutathione peroxidase, inhibits vinorelbine-induced neutropenia, and improves the inhibition of immune factor expression induced by vinorelbine, thus exhibiting comprehensive anti-aging effects.

This invention provides a strain of *Lactobacillus plantarum*, and discloses the fermentation product of *Eucommia ulmoides* leaves, *Rehmannia glutinosa*, dried ginger, and *Dendrobium officinale* obtained by fermenting these herbs, along with its preparation method and applications. This fermentation product can directionally activate the phenylpropane biosynthetic pathway, inducing the generation of characteristic small molecule metabolites of polyphenols, particularly sanguranyl sulfadiazine (SSA). Moreover, compared to the raw materials, the fermentation product contains more active small molecule metabolites. Finally, experiments on zebrafish modeled with prednisone demonstrated that the fermentation product can significantly promote bone formation. The fermentation product mainly restores the expression levels of related genes to normal zebrafish gene expression levels by regulating these genes. Therefore, the fermentation product can be used to prepare foods or medicines that improve bone health.

Compositions and methods are described herein that are useful for the detection of prostate cancer progression by determining the expression levels of genes associated with metastatic PCa. Based on the expression of IL-6, SELE, FOSB, NRK, NFRB2, FOXP3, ARG1, CEBPDP, TNFα, ADAMTS4, PENK, FOSL1, DUSP1, ACTA1, AGT, ATF3, CDK1, CXCL8, SELP, VCAN, TFP12, or NR4A3 genes or any combination thereof, treatment of PCa with SELE agonists, TNFα antagonists, and / or immune checkpoint inhibitors (ICIs) are shown to be effective.

This application relates to the field of biotechnology, and more particularly to the application of a U2AF homologous motif kinase 1 in gastric cancer cells. The application includes using UHMK1 as a regulator to modulate the proliferation of gastric cancer cells by regulating the zDHHC17-mediated palmitoylation-modified AKT / mTOR signaling pathway. This application is based on previous research finding that UHMK1 is highly expressed during the proliferation of gastric cancer cells. Knocking down or interfering with UHMK1 expression can clearly observe apoptosis in gastric cancer cells. Subsequent analysis of the expression levels of genes related to the UHMK1 signaling pathway, as well as changes in zDHHC17 expression levels, suggests that UHMK1 drives gastric cancer cell proliferation by regulating the zDHHC17-mediated palmitoylation-modified AKT / mTOR signaling pathway, thus filling a gap in the correlation between UHMK1 and gastric cancer.

This invention belongs to the field of molecular breeding technology and provides a method based on... C12orf4 Molecular breeding methods and applications for genetic variations of the eQTL gene in eggshell strength and anti-aging resistance. This invention utilizes molecular marker combinations... C12orf4 Gene expression levels have a regulatory role; the molecular marker combination is located at Chr1:72995066, Chr1:73250348, and Chr1:73614612 loci in the chicken genome, respectively. This invention, through multidimensional association analysis integrating genomic, transcriptomic, and phenotypic data, identified and constructed a key gene that can prospectively and systematically reflect the process of tissue functional decline in laying hens. C12orf4 The molecular marker combinations provided by this invention offer a new technical means for the accurate assessment of aging-related traits in laying hens and for molecular breeding, and have significant application value and industrial significance.

The application discloses a liver cancer prognosis marker and application thereof, and the liver cancer prognosis marker comprises TXNRD1, HAO1, C1S, OPTN and SNX6. The application provides a group of liver cancer prognosis markers, and a liver cancer patient prognosis prediction model is constructed based on the liver cancer prognosis markers. The liver cancer prognosis markers are obtained through comprehensive gene expression levels, single cell analysis and spatial transcriptome analysis, and the diversity of the data set and the large sample quantity improve the reliability and universality of the liver cancer prognosis markers and the liver cancer patient prognosis prediction model.

This invention discloses the application of protein GhRD21A in improving biological drought resistance. The amino acid sequence of protein GhRD21A is shown in SEQ ID No. 2. The plasmid pYES2-NTB- GhRD21A Transform the yeast strain to obtain GhRD21A Yeast BY4741-pYES2-NTB- with significantly increased gene expression levels GhRD21A The pYES2-NTB vector was transformed into a yeast strain to obtain yeast BY4741-pYES2-NTB containing the pYES2-NTB vector. GhRD21A Both *Bacillus subtilis* and yeast BY4741-pYES2-NTB can grow in medium containing PEG3350, but the former shows significantly stronger growth than the latter. Therefore, overexpression... GhRD21A GhRD21A Genes can enhance the drought resistance of yeast. This invention has significant application value.

This invention provides a hypoglycemic subspecies of Bifidobacterium animalis (Liver) and its applications. The Bifidobacterium animalis (Liver) subspecies is named Tianshan-1 and is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 35821 on September 5, 2025. Cell experiments show that Tianshan-1 significantly promotes the secretion of GLP-1 from human colorectal adenocarcinoma cells NCI-H716 and upregulates GCG gene expression levels. Animal experiments confirm that this strain significantly increases serum GLP-1 concentration in diabetic mice and reduces fasting blood glucose and glycated hemoglobin levels, with no significant difference in hypoglycemic effect compared to the positive control. This strain is a food-grade safe probiotic with a clear mechanism of action and stable effects, providing a safe, long-lasting, and industrially viable new probiotic resource for diabetes prevention and treatment, with broad application prospects.

This invention belongs to the field of biotechnology, specifically relating to the acetylcholine receptor of the root-knot nematode *Eupolyphaga sinensis*. unc38 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Eriocheir sinensis* root-knot nematode, this invention is based on the *Eriocheir sinensis* acetylcholine receptor... unc38 Gene SEQ ID NO:1 was used to design and synthesize gene fragment SEQ ID NO:5 and the corresponding dsRNA. The synthesized dsRNA was used to soak second-instar root-knot nematodes. unc38 Gene expression levels were downregulated, resulting in a mortality rate of 68.9% at 60 hours, and complete death of nematodes at 120 hours. Immersion unc38 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 57%, and the number of females, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.

In the present invention, the mRNA of the genes VTN, CXCL5, NEDD4, LCN2, SYTL4, TNFR2, and SERPING1, or the proteins encoded by these genes, exhibit differential expression levels in radioresistant lung cancer cells compared to control lung cancer cells and have a close correlation with a decrease in the survival rate of lung cancer patients. Accordingly, this preparation for measuring the expression levels of the mRNA of the genes selected from the group consisting of VTN, CXCL5, NEDD4, LCN2, SYTL4, TNFR2, and SERPING1 of the present invention or the expression levels of the proteins encoded by the genes can effectively diagnose radiation resistance in lung cancer or prognose radiation therapy for lung cancer, whereby it is possible to determine whether to perform radiation therapy and the optimal dose of radiation therapy, thus improving the efficiency and therapeutic outcomes of radiation therapy for lung cancer.

This invention discloses an enhancer that regulates chicken PGC-1α gene expression and its applications, belonging to the field of bioengineering. The nucleotide sequence of the enhancer is shown in SEQ ID NO: 1. This invention further discloses biomaterials containing this enhancer and a method for preparing cell lines with the enhancer knocked out. A dual-luciferase reporter gene system confirmed its significant activity in DF1 and ICP2 cells. Knocking out this enhancer downregulates PGC-1α gene expression in chicken preadipocytes, significantly promoting cell differentiation and lipid droplet formation. This enhancer can be used to prepare biological agents that regulate PGC-1α gene expression levels and chicken preadipocyte differentiation. It can also be applied to the genetic improvement of chicken fat traits and to the preparation of models for studying related gene function and cell differentiation mechanisms. This invention provides new targets and technical support for improving chicken fat traits.

This invention belongs to the field of biological detection technology, and particularly relates to a reproductive tract HPV-mRNA-E6 / E7 detection system and method based on nanopore sequencing. The system includes a sample pretreatment module, an RNA extraction and purification module, a reverse transcription module, a nanopore sequencing module, and a bioinformatics analysis module. The method includes the following steps: (1) reproductive tract sample collection and pretreatment; (2) extraction and purification of HPV-mRNA in the sample; (3) reverse transcription of the purified HPV-mRNA into cDNA; (4) nanopore sequencing of the cDNA to obtain raw sequencing data; (5) filtering, comparing, and annotating the sequencing data through the bioinformatics analysis module to achieve HPV type identification and quantitative analysis of E6 / E7 gene expression levels. This invention utilizes the advantages of nanopore sequencing, such as long read length and real-time detection, combined with the specificity of E6 / E7 mRNA as a biomarker of active infection. It can directly detect latent and active HPV infection, improve detection sensitivity, accurately distinguish infection types, and shorten the detection cycle. It enables rapid, accurate, and high-throughput detection of active HPV infection in the genital tract, providing reliable technical support for early screening, disease monitoring, and prognostic assessment of HPV-related diseases such as cervical cancer.

This invention provides the application of FSD2 in regulating resistance to rice sheath blight and bacterial leaf blight, belonging to the field of gene breeding technology. This invention provides... FSD2 The application of genes and / or encoded proteins in regulating resistance in rice to infectious diseases caused by pathogenic microorganisms, the aforementioned FSD2 Genes are involved in regulating rice resistance to sheath blight and bacterial leaf blight. This invention obtains these genes through genetic transformation. FSD2 Overexpressing plants, compared with wild-type plants, showed that overexpressing plants... FSD2 The gene expression level was significantly higher than that of the wild type; after inoculation with *Rhizoctonia solani* and *Bacterium oxysporum*, the overexpressing plants showed significantly enhanced resistance to *Rhizoctonia solani* and *Bacterium oxysporum*. This invention provides a theoretical basis for elucidating the disease-resistant properties of plants, and can also be applied to the screening and creation of disease-resistant rice materials, laying a germplasm foundation for future variety improvement.

This invention discloses a regulatory gene BpMYB090 for the development of epidermal hairs on paper mulberry leaves, its expressed protein, and its applications, belonging to the field of plant genetic engineering technology. The nucleotide sequence of this gene, BpMYB090, is shown in SEQ ID NO.1. This gene belongs to the ninth subgroup of the R2R3 MYB transcription factor family, MIXTA class, and its expression level is significantly positively correlated with the density of epidermal hairs on paper mulberry leaves. Overexpression of BpMYB090 in Arabidopsis thaliana significantly increases the number of epidermal hairs and restores the epidermal hair-deficient phenotype of the Arabidopsis thaliana mutant gl1, confirming that BpMYB090 is a positive regulator of epidermal hair formation. This invention reveals for the first time a key regulatory gene for the development of epidermal hairs on paper mulberry leaves. Furthermore, based on the positive regulatory function of this gene, new hairless forage paper mulberry varieties can be bred through gene silencing or editing techniques, possessing significant theoretical value and application prospects.

The application belongs to the technical field of biological medicine, and particularly relates to application of a CLIC5 gene in preparation of a drug for preventing and / or treating intestinal inflammation. The application provides application of the CLIC5 gene in preparation of a drug for preventing and / or treating intestinal inflammation, wherein a nucleotide sequence of the CLIC5 gene is shown as SEQ ID NO:1. The application finds that intestinal epithelial cell-specific knockout of the CLIC5 can reduce a serum endotoxin level, improve intestinal epithelial barrier function, increase a content of butyric acid, reduce a serum inflammatory factor level, and also reduce a gene expression level of an inflammatory factor in an ileum and a colon. The application first discloses that intestinal epithelial cell-specific knockout of the CLIC5 can effectively prevent or treat intestinal inflammation and reduce a body inflammatory factor level, thereby providing a new target and method for treating human intestinal inflammation.

This invention discloses VvENO2 The application of genes in regulating plant fruit development belongs to the field of plant genetic engineering technology. Previous research in this invention found that hydrogen peroxide (H2O2) treatment significantly promotes grape fruit ripening. Transcriptome analysis of H2O2-treated grapes revealed… VvENO2 Gene expression levels decreased. To further clarify... VvENO2 The role of the gene in plant fruit development was investigated through subcellular localization experiments and promoter activity analysis, demonstrating its nucleus location and that its promoter can activate the GUS reporter protein. Furthermore, treatment with ABA and H2O2 enhanced its activity. In addition, transgenic Arabidopsis and transgenic grape were constructed, and phenotypic observations confirmed... VvENO2 Genes negatively regulate the ripening of plant fruits. This invention provides a new regulatory site for breeding early-maturing plant varieties.

This invention provides CaCA1 The application of genes in the resistance of chili peppers to low temperature and low light stress falls under the field of gene breeding technology. Under low temperature and low light stress... CaCA1 Transient overexpression can slow down wilting in pepper plants, increase the content of photosynthetic pigments, transpiration rate, antioxidant enzyme activity and their gene expression levels, soluble protein, free proline and mineral element content in pepper leaves, and reduce intercellular CO2 concentration, malondialdehyde and heat dissipation per unit area. Transient silencing was performed using VIGS technology. CaCA1 Genetic factors reduced the plant height, stem diameter, biomass, and root morphology parameters of chili peppers, and caused stomatal closure, resulting in a decrease in overall fluorescence intensity, photosynthetic performance index, and element content. CaCA1 Transient overexpression of the gene in peppers can alleviate plant wilting and improve resistance to low temperature and low light stress, while silencing the gene... CaCA1 Genetic modification can weaken its resistance.

This invention proposes a pre-trained paradigm for genome characterization and gene regulation analysis, including: acquiring gene expression, genome sequence, and regulatory factor expression data from biological samples; standardizing gene expression data; segmenting and encoding genome sequence and regulatory factor expression data; using masked self-supervised learning to encode two types of sequence feature representations separately through a sequence coding model; aligning and fusing features using an attention fusion mechanism to generate cell type-specific genome region representations, predicting gene expression levels, and optimizing model parameters; and performing gene regulatory network inference or genome function prediction based on the optimized model's genome region representations to obtain gene regulatory relationship analysis results. This invention achieves precise fusion of gene features and accurate prediction of expression levels, improving the accuracy of gene regulatory relationship analysis and providing an efficient method for genome function research.

This invention relates to the field of plant molecular breeding technology, and discloses a tomato NOR‑like1 The application of genes in breeding tomato varieties that are easy to deseed and process, the aforementioned NOR‑like1 The gene is an encoding a NAC transcription factor, the coding sequence of which is shown in SEQ ID NO: 1, or a nucleic acid sequence that has at least 90% sequence identity with the sequence shown in SEQ ID NO: 1 and has the function of regulating the development of the trichomes of tomato seed coat; by reducing or eliminating the above... NOR‑like1 Breeding goals are achieved by adjusting gene expression levels or protein function in tomato plants. The resulting tomato plants exhibit the absence of seed coat hairs on the seed surface, preventing adhesion between adjacent seeds, and producing large fruits. This invention fundamentally eliminates seed coat hairs on tomato seeds at the genetic level, and has broad application potential in breeding tomato varieties with easily separable seeds from the pulp and easily separable seeds.