276 results about "Gene expression level" patented technology

A spatial omics multi-modal fusion method under a single cell level comprises the following steps: extracting spatial morphological characteristics of differential expression genes and cell nucleuses from spatial transcriptome data, single cell sequencing data and histological images, and realizing field adaptation among different platforms by using a conditional variation auto-encoder. And based on a probability inference model, fusing spatial transcriptome expression, unicellular omics and morphological characteristics, and jointly inferring the type and gene expression level of each cell. A spatial cell network is constructed through a graph attention mechanism, and spatial diffusion and recognition of cell types in a full slice range are realized. In combination with a multi-omics enhancement module, undetected gene and protein expression is completed based on expression similarity, and prediction consistency is improved through spatial correction. According to the method, high-resolution reconstruction of single-cell multi-omics information in a three-dimensional space is realized, the information coverage and spatial resolution of spatial omics data are improved, and an efficient and low-cost solution is provided for spatial biology and precise medical research.

The invention provides an ovarian cancer accurate diagnosis and personalized treatment method based on data integration, and the method comprises the steps: employing a feature extraction algorithm to generate a multi-dimensional feature vector set containing a gene expression level, an image texture parameter, a symptom score and a pathological classification according to a structured data set; performing dimension reduction processing on the multi-dimensional feature vector set through a feature fusion module to obtain a low-dimensional feature representation vector; performing grouping and risk prediction on the patient data by adopting a classification algorithm according to the low-dimensional feature representation vector, and generating a patient subgroup classification and disease risk layering result; according to the patient subgroup classification and the disease risk layering result, a scoring model is adopted to generate a diagnosis scoring result and a personalized treatment recommendation scheme, and a diagnosis report is output.

The invention relates to the technical field of biological medicine, in particular to an ulcerative colitis disease clearance evaluation molecular marker and application thereof. By detecting the expression level of the HMGCS2 gene in a sample, the ulcerative colitis disease clearance state and disease activity period can be evaluated, and the method is used for curative effect monitoring, recurrence prediction and individualized treatment strategy formulation of ulcerative colitis and has important clinical application value and market prospect.

The invention relates to the technical field of agricultural biological genes, and discloses a method for improving citrus canker resistance based on a CsKCS2 gene, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsKCS2 gene in citrus plants. The method specifically comprises the following steps: (1) cloning a citrus CsKCS2 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsKCS2 gene expression level. By cloning the coding sequence of the citrus CsKCS2 gene, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsKCS2 overexpression transgenic plant can be reduced to 73.3% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the application prospect is broad. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The invention provides an innovative model based on the correlation between the PLEKHA4 gene expression level and the low-grade glioma radiotherapy sensitivity and a prediction method. According to the model, a multi-factor Logistic regression analysis framework is constructed, and the PLEKHA4 gene expression level and key clinical pathological parameters are organically combined, so that a radiotherapy sensitivity prediction model is established. The method comprises a series of steps of sample collection, gene expression detection, clinical information collection, model calculation, result interpretation and the like, and can realize accurate prediction of radiotherapy response of low-grade glioma patients. Compared with the prior art, the method has the remarkable advantages of simplicity and convenience in operation, high prediction accuracy, high clinical transformability and the like. The method has great potential in the aspect of guiding individualized radiotherapy scheme formulation, can significantly improve the treatment effect, and has important value for medical application. Besides, the model can be optimized through further clinical verification, so that clinical practice can be better served, and the life quality and prognosis effect of patients are improved.

The invention relates to the technical field of biological medicine, in particular to application of a creatine related gene detection reagent in preparation of breast cancer prognosis products. The creatine related gene is an IGFBP1 gene and / or a TBC1D4 gene. According to the method, the expression levels of IGFBP1 and TBC1D4 genes in breast cancer patient samples are detected, a risk score is calculated by using a random survival forest (RSF) model, and the one-year, two-year and three-year survival rates of breast cancer patients are accurately predicted according to the risk score result. Meanwhile, the invention proves that IGFBP1 and TBC1D4 genes can be used as potential targets for breast cancer treatment, the IGFBP1 and fulvestrant as well as the TBC1D4 and vinblastine have stable binding capacity, and a new basis is provided for selection of individualized treatment medicines for breast cancer.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

This invention provides a method and apparatus for splitting single-cell mixed sample sequencing data, relating to the field of biotechnology. The splitting method includes: capturing and sequencing single-cell suspensions using a single-cell platform; then performing reference genome alignment, cell identification, and gene expression level quantification on the sequencing data using Cellranger; splitting the cell data identified in step a into two groups of cell data for different sexes based on SNP locus information from the 1000 Genomes Project; and distinguishing the two groups of cell data from male or female samples based on the proportion of sex-specific genes expressed in the two groups of cell data. This splitting method eliminates the need for additional experimental operations such as protein labeling and genome sequencing, and can provide accurate and reliable data splitting even when individual SNP information is unavailable.

The invention relates to an application of a DIO1 gene in a liver in controlling the body color of a turbot, and belongs to the field of molecular biology, and the application method is as follows: the turbot with golden body color is obtained by overexpressing the DIO1 gene in the liver of the turbot; the amino acid sequence of the protein coded by the DIO1 gene is as shown in SEQ ID NO. 1. The invention discovers that the gene expression level change in the liver can cause the change of the blood hormone level by influencing the thyroid hormone pathway in the turbot for the first time, thereby influencing the color formation of the skin; therefore, the formation of the target body color of the scophthalmus maximus can be controlled by controlling the gene expression level, so that the market value of the scophthalmus maximus is improved.

The application relates to the technical field of biological medicine, and discloses application of a reagent for detecting CBS gene expression level in preparation of a breast cancer diagnosis and / or prognosis product. The nucleotide sequence of the CBS gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded CBS protein is shown as SEQ ID NO. 2. The experimental results of the application show that the expression of the CBS protein in breast cancer tissues is significantly higher than that in paracancerous tissues, and the CBS protein can be used as a biomarker for breast cancer diagnosis. Meanwhile, the ROC curve evaluation of the CBS protein expression amount on the prognosis results of breast cancer patients shows that the AUC value reaches 0.841, which also shows that the CBS protein can be used as a marker for the prognosis of breast cancer patients, and the CBS protein has important diagnostic significance for the prognosis of breast cancer.

The invention provides a method for merging expression values based on an MTX family and a kit for predicting prognosis of thyroid cancer, the kit detects the transcriptional level expression quantity of the MTX gene family by combining an RT-qPCR technology with a specific primer, and the expression quantity data is substituted into a prognosis prediction model, so that the prognosis of a thyroid cancer patient is realized. Particularly, the prognosis evaluation of BRAF V600E mutant thyroid cancer patients is realized. Experiments prove that the expression level of the MTX gene family is related to thyroid cancer driving gene BRAF V600E mutation, and the prognosis of a patient is influenced by influencing the electron transfer function of the BRAF V600E mutation thyroid cancer patient, so that the MTX gene expression level detection can be used as a prognosis prediction index of the BRAF V600E mutation thyroid cancer patient, and the prognosis of the BRAF V600E mutation thyroid cancer patient is influenced. And a basis is provided for selection of operation modes of thyroid cancer patients.

The present application belongs to the field of biomedical technology, and particularly relates to an axial length and growth trend evaluation method of eye axis based on PDGFRA gene and a PDGFRA enzyme activity control agent. The method comprises the following steps: 1) collecting an eye biological sample of a subject; 2) based on the expression level of PDGFRA gene of platelet-derived growth factor receptor alpha in the collected biological sample; 3) based on the expression level, calculating the axial length of the eye axis according to the quantitative relationship between the expression amount of PDGFRA and the axial length of the eye axis, and predicting the growth trend of the axial length of the eye axis through additional calculation. The technical scheme of the present application can effectively detect and evaluate the axial length of the eye axis, effectively judge the growth trend of the axial length of the eye axis, and directly and efficiently control the axial length of the eye axis and its growth trend through the existing drug components.

The invention provides an application of an Opnlrp12 gene in prevention and treatment of torus schlegeli virus and / or bacterial infection, and belongs to the technical field of gene engineering. Through targeted knockout of Opnlrp12, it is found that the expression level of a disease-resistant gene is also reduced, and therefore it is proved that Opnlrp12 can effectively inhibit infection caused by viruses and / or bacteria, and the survival rate of oplegnathus punctatus is increased. By means of the method, control over inflammatory diseases caused by iridovirus and / or vibrio harveyi can be achieved, and a target spot is provided for treatment of iridovirus infection of oplegnathus punctatus.

The invention belongs to the technical field of biology, and relates to application of a ZmCASPL11 gene in improving heat resistance of plants. The ZmCASPL11 gene encodes a protein of a membrane protein (CASPL) family related to a Kjeldahl band structure, the gene expression level of the ZmCASPL11 gene under high-temperature stress is remarkably increased, and a mutant zmcaspl11 of the gene shows a high-temperature sensitive phenotype. The ZmCASPL11 gene disclosed by the invention is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmCASPL11 gene to heat stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn heat resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new heat-resistant corn strains.

The present invention addresses the problem of providing: a method for producing useful substances in animal cells, the method increasing the expression of a target gene; animal cells in which the expression of the target gene is increased; a method for producing such animal cells; and a kit for expressing the target gene in animal cells. The present invention provides a method for producing useful substances, the method comprising: introducing into animal cells (A) a nucleic acid containing (a) an exogenous target gene encoding the useful substance, (b) an exogenous constitutive promoter, and (c) a recognition sequence to which a binding substance can bind, and (B) a complex of three or more transcriptional activators and a binding substance, or a nucleic acid encoding the complex; and culturing the resulting animal cells.

This invention belongs to the field of molecular breeding technology and provides a method based on... YIPF1 Molecular breeding methods and applications for inhibiting egg production decline in laying hens using the eQTL gene. This invention combines molecular markers with... YIPF1 Gene expression levels have a regulatory role; the combined molecular markers are located at Chr8:24801464 and Chr8:24802450 loci in the chicken genome, respectively. This invention breaks through the traditional single-trait research paradigm, integrating multidimensional omics and phenotypic data to identify genes from a systems biology perspective. YIPF1 The gene and its regulatory site combination, Chr8:24801464 and Chr8:24802450, serve as key targets in the process of tissue functional decline in laying hens. This gene possesses cross-tissue, dynamically correlated early warning characteristics, providing a reliable tool for early assessment and precise breeding of anti-aging traits in laying hens, and has significant application value for achieving the goal of an ultra-long laying cycle.

This invention belongs to the field of intelligent medicine, specifically relating to a method, device, medium, and program product for predicting molecular subtypes of lung adenocarcinoma. The method includes acquiring gene data of a sample to be tested; processing the gene data of the sample to obtain key characteristic genes; the key characteristic genes include NDNF, CCNA2, or RNASE1; inputting the key characteristic genes into a classifier to obtain classification results of LUAD-C2 subtype or non-LUAD-C2 subtype; the LUAD-C2 subtype has high apoptosis; the non-LUAD-C2 subtype has low apoptosis. This application has obtained two different molecular subtypes through research on lung adenocarcinoma and has achieved subtype prediction through gene expression levels; the two different subtypes correspond to different apoptosis rates.

An optimized AAV vector for gene therapy of muscular dystrophy is provided. The optimized AAV vector includes a plurality of mutant AAV9 vectors and a microdystrophin transgene (p.AAV-CBA-kozak-μDys). The optimized AAV vector demonstrate increased transduction efficiency, gene expression levels, and can potentially achieve optimal therapeutic efficacy in humans at lower vector doses. The optimized AAV vector also demonstrate improved transduction and dystrophin gene expression in a mice model of Duchenne muscular dystrophy.

The application discloses an OsDAXX1 protein and application of an encoding gene thereof in regulating plant growth and development. The application provides application of the OsDAXX1 protein or the OsDAXX1 gene in regulating plant height and / or growth period and / or fertility. The regulation means that the content of the OsDAXX1 protein / OsDAXX1 gene expression amount is reduced, the plant height is reduced and / or the growth period is increased and / or the fertility is reduced. The application can cause the rice to exhibit the following phenotypes: the plant height is reduced and / or the growth period is increased and / or the fertility is reduced by introducing a gene editing vector to down-regulate the expression level of the OsDAXX1 protein and the OsDAXX1 gene in the rice. The application lays a foundation for finding a simpler idea and method for creating a product resistant to lodging and male sterile.

This invention discloses a method for delaying leaf senescence in Paspalum notatum under salt stress. The method includes the following steps: plant material culture and salt stress treatment; H2O2 content determination; DAB staining; chlorophyll content and Fv / Fm value determination; lipidomics data determination and analysis; endogenous hormone level analysis; real-time quantitative PCR analysis of gene expression levels; and transcriptomics data analysis. This invention discovers that root-derived H2O2 in Paspalum notatum under salt-driven conditions delays leaf senescence by participating in the regulation of jasmonic acid. The role of root-derived H2O2 was studied using diphenylthioiodine (DPI), a specific inhibitor of NADPH oxidase. Furthermore, the influence of the presence or absence of root-derived H2O2 on the expression of genes related to jasmonic acid synthesis precursors was investigated at the gene expression level. Mass spectrometry-based omics technology and data analysis are the first key technologies for implementing this project. Lipidomics, as one of the most important branches of metabolomics, can perform qualitative and quantitative analysis at the level of individual lipid species.

The invention discloses a solid tumor taxane / immunotherapy response evaluation system based on integrated machine learning and a working method thereof, and belongs to the technical field of bioinformatics and artificial intelligence crossing. The method comprises the following steps of: 1, preprocessing acquired solid tumor taxane chemotherapy / immunotherapy response transcriptome data or a source data set, and dividing a training set and a verification set; step 2, extracting overlapped genes as candidate genes from the gene expression level based on the preprocessed data; step 3, carrying out reprocessing based on the candidate genes; 4, constructing a consensus prediction model; 5, training the constructed model by adopting the training set in the step 3; and 6, verifying and screening the trained model to obtain an optimal scheme. According to the method, a stable and generalizable prediction tool for solving the problem of curative effect heterogeneity of complex combined treatment and how to accurately screen potential benefit people and avoid excessive treatment is filled.

This invention belongs to the field of agricultural biotechnology, specifically relating to a type of sisal. DREB Genes and their application in improving plant cold resistance. This invention provides sisal. DREB The gene expression level increased significantly after low-temperature stress, indicating a close relationship between its expression and the response of sisal to low-temperature stress. The study found that transgenic methods can significantly improve the expression level of sisal. DREB Genes were overexpressed, along with sisal DREB Increased gene expression levels led to improved cold resistance in transgenic Arabidopsis thaliana. Overexpression of sisal in plants also improved cold resistance. DREB The discovery that genes can enhance plant cold resistance can be used to breed new varieties or seedlings of cold-resistant plants, providing a theoretical basis and genetic resources for genetic breeding, which is of great significance to agricultural production.

The invention provides an ethylene biosynthesis RhACO4 gene for regulating and controlling the aging of Chinese rose flowers and application of the ethylene biosynthesis RhACO4 gene, and belongs to the technical field of gene engineering. The coding nucleotide sequence of the RhACO4 gene is as shown in SEQ ID NO. 1. According to the invention, the fact that two genotypes (ProRhACO4A and ProRhACO4B) exist in an RhACO4 promoter region in 358 tetraploid Chinese rose populations is found for the first time, and the difference is that whether a section of GA dinucleotide tandem repeat sequence (GA-peak) which is 11 times is contained or not. The ProRhACO4A type carrying GA-peak is significantly related to a lower gene expression level, a lower endogenous ethylene synthesis amount and a longer vase life. The invention provides a key gene target, a molecular marker and a technical path for cultivating a new variety of rosa chinensis with a long flowering period through molecular marker-assisted selection or genetic engineering means.

The application provides a KRT31 gene knockout cell line and its application in promoting replication of viruses of the Picornaviridae family and / or production of vaccines of viruses of the Picornaviridae family, and belongs to the technical field of genetic engineering. The application provides an application of a KRT31 gene or a coded protein thereof as a target in preparation of a product for regulating replication of viruses of the Picornaviridae family and / or vaccine production. Up-regulation of the expression level of the KRT31 gene can inhibit replication of viruses of the Picornaviridae family, and down-regulation of the expression level of the KRT31 gene can promote replication of viruses of the Picornaviridae family. The application adopts sgRNA to knockout the KRT31 gene to prepare a cell line with lost function of a gene coded protein, so as to promote replication of viruses of the Picornaviridae family, improve virus titer and antigen yield, and help to efficiently prepare vaccines of viruses of the Picornaviridae family.

Provided herein are compositions and methods for treatment of Amyotrophic Lateral Sclerosis as well as Spinocerebellar Ataxia Type 2 by way of reducing levels of the ATXN2 gene expression. More specifically. miRNA compositions that target ATXN2 are able to reduce ATXN2 levels.

This invention belongs to the field of biomedicine and relates to the application of a detection reagent for a marker of mixed connective tissue diseases. This invention discloses... MEF2C Genes can serve as biomarkers for mixed connective tissue disease; they are found in the peripheral blood of patients with mixed connective tissue disease. MEF2C Gene expression levels were significantly higher in the group than in the healthy group. This invention provides a detection reagent for a mixed connective tissue disease marker, comprising an upstream primer with the nucleotide sequence shown in SEQ ID NO.3 and a downstream primer with the nucleotide sequence shown in SEQ ID NO.4, which can detect... MEF2C Gene expression levels, which can then be used to develop products for the early diagnosis of mixed connective tissue diseases.

The invention discloses a circular non-coding RNA (Ribonucleic Acid) molecule circSENP6 and application thereof. The nucleotide sequence of the circSENP6 is shown as SEQ ID NO. 1. The invention also discloses a preparation method of the circular non-coding RNA molecule circSENP6. The circular non-coding RNA molecule circSENP6 can be used as a diagnostic biomarker for coronary atherosclerosis, is used for biological identification and clinical detection of coronary atherosclerotic heart disease, and can be used for early discovering coronary heart disease patients, reducing the death rate of coronary heart disease and improving the prognosis of patients by using the expression level of the gene for diagnosis. At present, the main diagnosis method for coronary atherosclerotic heart disease has the risks of high operation danger coefficient, large radiation and the like, and by collecting peripheral blood of a patient for detection, the method has the advantages of small trauma, good repeatability and the like, and is easier to accept.