411 results about "Gene expression level" patented technology

A spatial omics multi-modal fusion method under a single cell level comprises the following steps: extracting spatial morphological characteristics of differential expression genes and cell nucleuses from spatial transcriptome data, single cell sequencing data and histological images, and realizing field adaptation among different platforms by using a conditional variation auto-encoder. And based on a probability inference model, fusing spatial transcriptome expression, unicellular omics and morphological characteristics, and jointly inferring the type and gene expression level of each cell. A spatial cell network is constructed through a graph attention mechanism, and spatial diffusion and recognition of cell types in a full slice range are realized. In combination with a multi-omics enhancement module, undetected gene and protein expression is completed based on expression similarity, and prediction consistency is improved through spatial correction. According to the method, high-resolution reconstruction of single-cell multi-omics information in a three-dimensional space is realized, the information coverage and spatial resolution of spatial omics data are improved, and an efficient and low-cost solution is provided for spatial biology and precise medical research.

The invention relates to the technical field of bioinformatics, and provides a crop whole genome phenotype prediction method and system fused with an environmental indicator gene, and the method comprises the following steps: collecting re-sequencing data, and carrying out genome comparison to obtain variation site data; performing whole genome association analysis by using the variation site data to obtain phenotype association site information; carrying out gene expression quantity measurement on samples of the crop population material in different environments to obtain gene expression quantity data; performing differential expression analysis on the gene expression quantity data to screen environmental indicator genes to obtain an environmental indicator gene set; constructing a phenotype prediction model of double-branch fusion; and predicting a to-be-predicted material through the phenotype prediction model to obtain phenotype prediction results for different environments. According to the method, environmental factors are incorporated into the whole genome selection model, so that the phenotype prediction precision in different environments is improved.

The invention relates to the technical field of plant genetic engineering, and particularly discloses an alfalfa salt-alkali-resistant and low-temperature composite stress-resistant gene MsNCED5 and application of encoded protein thereof in improving salt-alkali-resistant and low-temperature composite stress-resistant capability of plants. The nucleotide sequence of the gene disclosed by the invention is as shown in SEQ ID NO.1, the full length is 1833 bp, and 610 amino acids are encoded. According to the invention, an MsNCED5 overexpression vector is constructed and medicago sativa is converted, so that an MsNCED5-OE transgenic line is successfully created, and the MsNCED5 gene expression quantity of the MsNCED5-OE transgenic line is obviously increased compared with that of a wild type. Under the salt-alkali and low-temperature composite stress, the transgenic plant has stronger stress tolerance compared with the wild plant, which indicates that the overexpression of the MsNCED5 gene improves the resistance of the plant to the salt-alkali and low-temperature composite stress. The MsNCED5 gene disclosed by the invention provides an important theoretical basis for researching an alfalfa stress resistance molecular mechanism and breeding.

The invention belongs to the technical field of lake and reservoir water source risk prediction, and provides a lake and reservoir water source algae bloom risk early warning system based on a 16S / 18SrRNA gene expression quantity threshold, and the method comprises the following steps: S1, collecting a sample from a lake and reservoir water source surface layer water body; s2, using 7-gate water bloom algae specific primers for blue-green algae, green algae, diatom, euglena, dinoflagellate, chrysophyta and cryptoalga; s3, based on the qPCR standard curve, calculating the copy number of the 16SrRNA / 18SrRNA gene of the seven water bloom algae; s4, when the gene expression quantity of a certain algal bloom algae continuously reaches 106-7 copy number / mL for 7-12 days, determining that the algal bloom algae is in a window phase; according to the threshold value of the gene expression quantity of the water bloom algae 16SrRNA / 18SrRNA, the window period of water bloom algae cells can be accurately recognized, so that early warning of the algae bloom risk in the lake and reservoir water source is achieved, and early warning of algae bloom outbreak in the lake and reservoir water source is achieved one week or above in advance.

The invention provides an ovarian cancer accurate diagnosis and personalized treatment method based on data integration, and the method comprises the steps: employing a feature extraction algorithm to generate a multi-dimensional feature vector set containing a gene expression level, an image texture parameter, a symptom score and a pathological classification according to a structured data set; performing dimension reduction processing on the multi-dimensional feature vector set through a feature fusion module to obtain a low-dimensional feature representation vector; performing grouping and risk prediction on the patient data by adopting a classification algorithm according to the low-dimensional feature representation vector, and generating a patient subgroup classification and disease risk layering result; according to the patient subgroup classification and the disease risk layering result, a scoring model is adopted to generate a diagnosis scoring result and a personalized treatment recommendation scheme, and a diagnosis report is output.

The invention belongs to the technical field of biological genetic engineering, and relates to a high-risk myelodysplastic syndrome screening gene and a diagnostic kit thereof. The invention provides an application of detecting YTHDF2, METTL3 and NAT10 gene expression of bone marrow cells of high-risk MDS patients as a specific molecular marker for screening the high-risk myelodysplastic syndrome, and by detecting mRNA expression levels of the YTHDF2, METTL3 and NAT10 genes, the screening of the patients in the high-risk myelodysplastic syndrome is facilitated. Meanwhile, treatment and prognosis evaluation of patients with high-risk myelodysplastic syndromes can be better guided. Only the bone marrow fluid of the patient needs to be extracted and collected during bone marrow cytology examination of the patient, the patient compliance is good, the bone marrow fluid is selected from human bone marrow fluid mononuclear cells, the detection result is more accurate, and the method is suitable for clinical large-scale application.

The invention provides application of a CD47 gene in detection of pig litter size phenotype and application of an SNP site of the CD47 gene in gene expression regulation. It is found that the CD47 gene is regulated and controlled by the SNP site rs323354626, it is further found that the rs323354626 site and the phenotype of the total litter size of sows show the highest significance, and it is indicated that the CD47 gene and the total litter size of the sows have large correlation. In three main commercial varieties of pigs, namely Duroc, Landrace and Yorkshire, the CD47 expression level in testis tissues is negatively correlated with the litter size, which indicates that the expression mode of the CD47 gene may influence the reproductive performance of the pigs, and the litter size phenotype of the pigs can be detected by detecting the CD47 gene expression level in the testis of the breeding pigs or somatic cells of the sows. Or the litter size of the pigs is influenced by regulating and controlling the expression level of the CD47 gene through the rs323354626 site, and the discovery lays a foundation for improving the breeding efficiency of the pigs, especially for a breeding method for increasing the litter size.

The invention relates to the technical field of biological medicine, in particular to an ulcerative colitis disease clearance evaluation molecular marker and application thereof. By detecting the expression level of the HMGCS2 gene in a sample, the ulcerative colitis disease clearance state and disease activity period can be evaluated, and the method is used for curative effect monitoring, recurrence prediction and individualized treatment strategy formulation of ulcerative colitis and has important clinical application value and market prospect.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention provides application of sclareol in preparation of a medicine for preventing and treating varicella-zoster virus infection. A series of biological experiments show that the sclareol can obviously relieve the cytopathic effect caused by VZV infection under the concentration without obvious cytotoxicity; the gene expression quantity of VZV characteristic genes including an immediate early gene ORF62, an early gene ORF21 and a late gene ORF68 and the expression of an early protein IE62 and a late protein gE can be remarkably inhibited, so that the effect of resisting VZV infection is achieved. The results show that sclareol has obvious prevention and treatment effects on VZV, has small toxic and side effects, is safe and effective, and has an application prospect in preparation of drugs for treating and / or preventing VZV.

The invention relates to the technical field of genetic engineering, in particular to an agrobacterium tumefaciens-mediated radix pseudostellariae hairy root genetic transformation method and application thereof. The method comprises the following steps: preparing a radix pseudostellariae explant; activating and culturing agrobacterium rhizogenes; constructing an engineering bacterium containing a target gene recombinant vector; infecting explants and inducing hairy roots; carrying out resistance screening and sterilization on transgenic hairy roots; carrying out amplification culture on hairy roots; and carrying out RT-PCR detection. The hairy root genetic transformation method provided by the invention is simple and rapid to operate and high in gene expression level, and can provide technical support for gene function research. Meanwhile, by using the genetic transformation method provided by the invention, through overexpression of the radix pseudostellariae cyclic peptide coding gene, a transgenic material with significantly improved content of the pharmacodynamic substance cyclic peptide of the radix pseudostellariae can be obtained, and a research material is provided for researching a biosynthetic pathway of the cyclic peptide.

The invention relates to the technical field of agricultural biological genes, and discloses a method for improving citrus canker resistance based on a CsKCS2 gene, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsKCS2 gene in citrus plants. The method specifically comprises the following steps: (1) cloning a citrus CsKCS2 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsKCS2 gene expression level. By cloning the coding sequence of the citrus CsKCS2 gene, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsKCS2 overexpression transgenic plant can be reduced to 73.3% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the application prospect is broad. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The invention provides application of a CHD1L detection reagent in preparation of a drug-resistant breast cancer screening kit, and belongs to the field of biological medicine. Researches find that when the CHD1L gene expression level in cancer tissues of a breast cancer patient is high, the breast cancer patient is prone to drug resistance to paclitaxel drugs, and the treatment effect of the paclitaxel drugs is poor. The reagent for detecting the CHD1L gene expression level is used for preparing the drug-resistant breast cancer screening kit, can be used for auxiliary diagnosis of clinical breast cancer types, provides an effective basis for related treatment measures or decisions of patients, and has a good clinical application prospect.

The invention provides an innovative model based on the correlation between the PLEKHA4 gene expression level and the low-grade glioma radiotherapy sensitivity and a prediction method. According to the model, a multi-factor Logistic regression analysis framework is constructed, and the PLEKHA4 gene expression level and key clinical pathological parameters are organically combined, so that a radiotherapy sensitivity prediction model is established. The method comprises a series of steps of sample collection, gene expression detection, clinical information collection, model calculation, result interpretation and the like, and can realize accurate prediction of radiotherapy response of low-grade glioma patients. Compared with the prior art, the method has the remarkable advantages of simplicity and convenience in operation, high prediction accuracy, high clinical transformability and the like. The method has great potential in the aspect of guiding individualized radiotherapy scheme formulation, can significantly improve the treatment effect, and has important value for medical application. Besides, the model can be optimized through further clinical verification, so that clinical practice can be better served, and the life quality and prognosis effect of patients are improved.

The invention relates to the technical field of primary local hyperhidrosis, in particular to a biomarker for detecting primary local hyperhidrosis and application of the biomarker, and the biomarker comprises but is not limited to one or more of the ratio of Th17 cells to Treg cells, IL-17A, IL-6, ROR gamma t and FoxP3. According to the invention, a multi-dimensional detection system covering PFH core pathological links (immune disorder, hyperactivity of sympathetic nerves and hyperfunction of sweat gland) is constructed by integrating immune related markers such as Th17 / Treg cell ratio, IL-17A, IL-6, ROR [gamma] t, FoxP3 and the like and combining the concentrations of peripheral blood epinephrine (E) and noradrenaline (NE) and the gene expression levels of sweat gland tissues AQP5 and Cacna1c. And a potential molecular target is provided for developing a novel therapeutic strategy (such as specific blocking of an IL-17 signal channel or enhancement of a Treg function) of a targeted Th17 / Treg axis.

The invention belongs to the technical field of biological medicine, and particularly relates to diagnosis and pharmaceutical application of SMOC2. Compared with normal people, the expression level of the SMOC2 gene in the heart of a clinical heart failure patient is increased, and the SMOC2 as a marker has clinical value in the diagnosis of cardiac hypertrophy and heart failure; in addition, by inhibiting the expression of the SMOC2 gene, the cardiac hypertrophy progress of the pressure overload model mouse can be delayed, and then the occurrence of heart failure is prevented. Therefore, a reagent for detecting the expression level of the SMOC2 gene can be used for preparing a reagent for detecting cardiac hypertrophy or heart failure, and an SMOC2 gene expression inhibitor can be used for preparing a medicine for preventing, relieving and / or treating cardiac hypertrophy.

The invention relates to the technical field of biological medicine, in particular to application of a creatine related gene detection reagent in preparation of breast cancer prognosis products. The creatine related gene is an IGFBP1 gene and / or a TBC1D4 gene. According to the method, the expression levels of IGFBP1 and TBC1D4 genes in breast cancer patient samples are detected, a risk score is calculated by using a random survival forest (RSF) model, and the one-year, two-year and three-year survival rates of breast cancer patients are accurately predicted according to the risk score result. Meanwhile, the invention proves that IGFBP1 and TBC1D4 genes can be used as potential targets for breast cancer treatment, the IGFBP1 and fulvestrant as well as the TBC1D4 and vinblastine have stable binding capacity, and a new basis is provided for selection of individualized treatment medicines for breast cancer.

The present invention belongs to the field of biological genetic engineering technology and relates to high-risk myelodysplastic syndrome screening genes and diagnostic kits. The present invention provides an application of detecting the expression of YTHDF2, METTL3, and NAT10 genes in bone marrow cells of high-risk MDS patients as a specific molecular marker for screening high-risk myelodysplastic syndrome. By detecting the expression levels of YTHDF2, METTL3, and NAT10 genes mRNA, it helps to screen patients with high-risk myelodysplastic syndrome and better guide the treatment and prognosis evaluation of patients with high-risk myelodysplastic syndrome. The present invention only needs to extract the patient's bone marrow fluid and collect it together when the patient undergoes bone marrow cytology examination. The patient compliance is good, and the material is taken from human bone marrow fluid mononuclear cells. The test results are more accurate and suitable for large-scale clinical application.

Provided are a GPC1 gene and the use thereof. The GPC1 gene encodes an amino acid sequence as shown in SEQ ID NO. 2. An increase in the single-gene expression level of the GPC1 gene leads to an increase in the whole-grain protein content in rice, and knockout of the gene results in a decrease in the whole-grain protein content in rice.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

This invention provides a method and apparatus for splitting single-cell mixed sample sequencing data, relating to the field of biotechnology. The splitting method includes: capturing and sequencing single-cell suspensions using a single-cell platform; then performing reference genome alignment, cell identification, and gene expression level quantification on the sequencing data using Cellranger; splitting the cell data identified in step a into two groups of cell data for different sexes based on SNP locus information from the 1000 Genomes Project; and distinguishing the two groups of cell data from male or female samples based on the proportion of sex-specific genes expressed in the two groups of cell data. This splitting method eliminates the need for additional experimental operations such as protein labeling and genome sequencing, and can provide accurate and reliable data splitting even when individual SNP information is unavailable.

The invention relates to application of an ILF3 gene in diagnosis or treatment of sepsis, and belongs to the technical field of biological medicines. It is found for the first time that the ILF3 gene is a macrophage-related differential expression gene of sepsis, the expression level of the ILF3 gene in plasma and alveolar lavage fluid of sepsis patients is obviously higher than that of healthy people, a primer pair capable of specifically recognizing and detecting the ILF3 gene is designed, a mouse with the ILF3 gene in macrophage being specifically knocked out is constructed, and the ILF3 gene in the macrophage is specifically knocked out. The invention also designs si-ILF3 capable of specifically interfering with the expression level of the ILF3 gene. The ILF3 gene is used as a sepsis diagnosis marker and a sepsis treatment target, the application of the ILF3 gene detection reagent in preparation of sepsis diagnosis products and the application of si-ILF3 in preparation of sepsis treatment drugs are provided, and the si-ILF3 gene detection reagent is used for further diagnosis and treatment of sepsis patients.

An information processing device for detecting expression-varying genes exhibiting specific expressions with respect to cell characteristics of interest on the basis of gene expression level data of a cell population in which a plurality of subtypes are mixed, said information processing device being provided with a processor for processing the expression-varying genes in which the expression-varying genes exhibit specific expressions with respect to the cell characteristics of interest. The processor performs a process for assigning a cluster to which a cell population is estimated to belong in a gene expression amount distribution for each of a plurality of candidate genes, which are candidates for expression-varying genes, to each sample in which the cell population is divided into two groups in accordance with cell characteristics of interest, and for each of the plurality of candidate genes on the basis of the result of the assignment of the clusters, performing a process for determining the expression amount of the candidate genes. A first probability distribution suitable for the distribution of the gene expression levels of the two groups is searched for.

The invention relates to an application of a DIO1 gene in a liver in controlling the body color of a turbot, and belongs to the field of molecular biology, and the application method is as follows: the turbot with golden body color is obtained by overexpressing the DIO1 gene in the liver of the turbot; the amino acid sequence of the protein coded by the DIO1 gene is as shown in SEQ ID NO. 1. The invention discovers that the gene expression level change in the liver can cause the change of the blood hormone level by influencing the thyroid hormone pathway in the turbot for the first time, thereby influencing the color formation of the skin; therefore, the formation of the target body color of the scophthalmus maximus can be controlled by controlling the gene expression level, so that the market value of the scophthalmus maximus is improved.

An information processing device executes processing of detecting a differential expressed gene that exhibits a specific expression with respect to a cell characteristic of interest, based on gene expression level data of a cell population in which a plurality of subtypes are mixed, and the information processing device includes a processor in which the processor assigns a cluster to which each sample of two groups obtained by dividing the cell population in accordance with the cell characteristic of interest is estimated to belong in a distribution of gene expression levels, to each sample, for each of a plurality of candidate genes that are candidates for the differential expressed gene, and searches for a first probability distribution that fits the distributions of the gene expression levels of the two groups for each of the plurality of candidate genes, based on an assignment result of the clusters.

The application relates to the technical field of biological medicine, and discloses application of a reagent for detecting CBS gene expression level in preparation of a breast cancer diagnosis and / or prognosis product. The nucleotide sequence of the CBS gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded CBS protein is shown as SEQ ID NO. 2. The experimental results of the application show that the expression of the CBS protein in breast cancer tissues is significantly higher than that in paracancerous tissues, and the CBS protein can be used as a biomarker for breast cancer diagnosis. Meanwhile, the ROC curve evaluation of the CBS protein expression amount on the prognosis results of breast cancer patients shows that the AUC value reaches 0.841, which also shows that the CBS protein can be used as a marker for the prognosis of breast cancer patients, and the CBS protein has important diagnostic significance for the prognosis of breast cancer.

The invention provides a method for merging expression values based on an MTX family and a kit for predicting prognosis of thyroid cancer, the kit detects the transcriptional level expression quantity of the MTX gene family by combining an RT-qPCR technology with a specific primer, and the expression quantity data is substituted into a prognosis prediction model, so that the prognosis of a thyroid cancer patient is realized. Particularly, the prognosis evaluation of BRAF V600E mutant thyroid cancer patients is realized. Experiments prove that the expression level of the MTX gene family is related to thyroid cancer driving gene BRAF V600E mutation, and the prognosis of a patient is influenced by influencing the electron transfer function of the BRAF V600E mutation thyroid cancer patient, so that the MTX gene expression level detection can be used as a prognosis prediction index of the BRAF V600E mutation thyroid cancer patient, and the prognosis of the BRAF V600E mutation thyroid cancer patient is influenced. And a basis is provided for selection of operation modes of thyroid cancer patients.

The present application belongs to the field of biomedical technology, and particularly relates to an axial length and growth trend evaluation method of eye axis based on PDGFRA gene and a PDGFRA enzyme activity control agent. The method comprises the following steps: 1) collecting an eye biological sample of a subject; 2) based on the expression level of PDGFRA gene of platelet-derived growth factor receptor alpha in the collected biological sample; 3) based on the expression level, calculating the axial length of the eye axis according to the quantitative relationship between the expression amount of PDGFRA and the axial length of the eye axis, and predicting the growth trend of the axial length of the eye axis through additional calculation. The technical scheme of the present application can effectively detect and evaluate the axial length of the eye axis, effectively judge the growth trend of the axial length of the eye axis, and directly and efficiently control the axial length of the eye axis and its growth trend through the existing drug components.

The invention provides an application of an Opnlrp12 gene in prevention and treatment of torus schlegeli virus and / or bacterial infection, and belongs to the technical field of gene engineering. Through targeted knockout of Opnlrp12, it is found that the expression level of a disease-resistant gene is also reduced, and therefore it is proved that Opnlrp12 can effectively inhibit infection caused by viruses and / or bacteria, and the survival rate of oplegnathus punctatus is increased. By means of the method, control over inflammatory diseases caused by iridovirus and / or vibrio harveyi can be achieved, and a target spot is provided for treatment of iridovirus infection of oplegnathus punctatus.

The invention belongs to the technical field of biology, and relates to application of a ZmCASPL11 gene in improving heat resistance of plants. The ZmCASPL11 gene encodes a protein of a membrane protein (CASPL) family related to a Kjeldahl band structure, the gene expression level of the ZmCASPL11 gene under high-temperature stress is remarkably increased, and a mutant zmcaspl11 of the gene shows a high-temperature sensitive phenotype. The ZmCASPL11 gene disclosed by the invention is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmCASPL11 gene to heat stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn heat resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new heat-resistant corn strains.