649 results about "Gene expression level" patented technology

A spatial omics multi-modal fusion method under a single cell level comprises the following steps: extracting spatial morphological characteristics of differential expression genes and cell nucleuses from spatial transcriptome data, single cell sequencing data and histological images, and realizing field adaptation among different platforms by using a conditional variation auto-encoder. And based on a probability inference model, fusing spatial transcriptome expression, unicellular omics and morphological characteristics, and jointly inferring the type and gene expression level of each cell. A spatial cell network is constructed through a graph attention mechanism, and spatial diffusion and recognition of cell types in a full slice range are realized. In combination with a multi-omics enhancement module, undetected gene and protein expression is completed based on expression similarity, and prediction consistency is improved through spatial correction. According to the method, high-resolution reconstruction of single-cell multi-omics information in a three-dimensional space is realized, the information coverage and spatial resolution of spatial omics data are improved, and an efficient and low-cost solution is provided for spatial biology and precise medical research.

The invention relates to the technical field of bioinformatics, and provides a crop whole genome phenotype prediction method and system fused with an environmental indicator gene, and the method comprises the following steps: collecting re-sequencing data, and carrying out genome comparison to obtain variation site data; performing whole genome association analysis by using the variation site data to obtain phenotype association site information; carrying out gene expression quantity measurement on samples of the crop population material in different environments to obtain gene expression quantity data; performing differential expression analysis on the gene expression quantity data to screen environmental indicator genes to obtain an environmental indicator gene set; constructing a phenotype prediction model of double-branch fusion; and predicting a to-be-predicted material through the phenotype prediction model to obtain phenotype prediction results for different environments. According to the method, environmental factors are incorporated into the whole genome selection model, so that the phenotype prediction precision in different environments is improved.

The invention provides a novel human-derived II-type collagen COLII-14K-H and a coding gene thereof, the coding gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. The invention further provides a preparation method of the novel human-derived II-type collagen COLII-14K-H. The invention also provides a preparation method of the human-derived II-type collagen COLII-14K-H and application of the human-derived II-type collagen COLII-14K-H in preparation of cartilage repair drugs, skin repair drugs and skin care products. The inventor-derived II-type collagen can obviously improve the gene expression level of the collagen, so that the content of the collagen in human dermal fibroblasts is improved, and the skin aging is delayed; meanwhile, the protein fragment can show a remarkable skin repairing effect at low concentration, provides a new treatment choice for skin repairing, and can be widely applied to the fields of skin repairing and nursing.

The invention provides an application of a PavERF110 gene in regulating and controlling the ripening direction of sweet cherry fruits, and belongs to the technical field of molecular breeding. The nucleotide sequence of the PavERF110 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the PavERF110 gene is as shown in SEQ ID NO.2. The invention also provides an overexpression vector and a VIGS gene silencing vector constructed by the gene, a construction method of the overexpression vector and the VIGS gene silencing vector, and a recombinant microorganism containing the vectors. Engineering bacteria containing related carriers are injected and infected at the equator of the fruits 25 days after the sweet cherries blossom, and it is found that overexpression of the PavERF110 gene can accelerate fruit coloring and increase the expression level of the mature related genes. New technical support and theoretical basis are provided for sweet cherry fruit quality improvement and ripening regulation, market requirements can be met, and meanwhile understanding of a fruit ripening transcription regulation mechanism is deepened.

The invention discloses application of a transcription factor CgGATA8 in adjusting lignin synthesis, and belongs to the technical field of genetic engineering breeding and fruit and vegetable preservation. According to the invention, the transcription factor CgGATA8 is instantaneously transferred into the juice sac of the Majia pomelo through an agrobacterium infection method, the lignin content in the over-expressed juice sac is obviously higher than that in no-load control, and autofluorescence signals are enhanced, secondary cell walls are thickened and the juice sac granulation symptom is aggravated in phloroglucinol dyeing and paraffin section at the same time. Molecular biology function verification shows that the Marijia pomelo transcription factor CgGATA8 can be combined with and activate a CgPOD16 promoter, the expression level of the CgPOD16 gene is improved, and the accumulation amount of lignin synthesis in fruits is improved, so that the CgGATA8 gene participates in regulation and control of synthesis and deposition of lignin in Marijia pomelo fruit juice cells, and has the function of promoting maturity and aging of picked fruits.

The invention relates to the technical field of plant genetic engineering, and particularly discloses an alfalfa salt-alkali-resistant and low-temperature composite stress-resistant gene MsNCED5 and application of encoded protein thereof in improving salt-alkali-resistant and low-temperature composite stress-resistant capability of plants. The nucleotide sequence of the gene disclosed by the invention is as shown in SEQ ID NO.1, the full length is 1833 bp, and 610 amino acids are encoded. According to the invention, an MsNCED5 overexpression vector is constructed and medicago sativa is converted, so that an MsNCED5-OE transgenic line is successfully created, and the MsNCED5 gene expression quantity of the MsNCED5-OE transgenic line is obviously increased compared with that of a wild type. Under the salt-alkali and low-temperature composite stress, the transgenic plant has stronger stress tolerance compared with the wild plant, which indicates that the overexpression of the MsNCED5 gene improves the resistance of the plant to the salt-alkali and low-temperature composite stress. The MsNCED5 gene disclosed by the invention provides an important theoretical basis for researching an alfalfa stress resistance molecular mechanism and breeding.

The invention discloses a human body electrocardio R wave detection method, and relates to the technical field of electrocardio signal detection.The system comprises the following components of gene expression data and electrocardio signal collection, specifically, gene expression level information of an individual whole genome is obtained through a gene detection technology, meanwhile, electrocardio signals are collected through a multi-channel electrocardio sensor, and the gene expression data and the electrocardio signals are obtained; according to the method, by introducing the gene expression data, personalized electrocardiogram R wave detection can be carried out on an individual with the tendency of specific gene variation or family genetic cardiovascular diseases, the conventional characteristics of the electrocardiogram signals are considered, information of the gene level is combined, and the method has the advantages of being high in accuracy and high in accuracy. Therefore, R waves can be recognized more accurately, false detection and missing detection are reduced, and the method has important significance in improving the accuracy of cardiovascular disease diagnosis, finding cardiovascular abnormality in an early stage and formulating a personalized treatment scheme.

A copy number variation detection method based on high-throughput transcriptome sequencing data of a single sample. The detection method comprises: first, aligning genome sequencing data to a human reference genome, and calculating the amount of data aligned to reads on each gene, so as to obtain an expression matrix; and then inputting the expression matrix into a detection model, so as to obtain a copy number variation detection result, wherein the detection model is obtained by means of performing training on the basis of a deep neural network model and by using pre-processed known database samples as a data set. By means of the detection method, the genome structure and variation of a single individual are better understood; with lower costs and in less time, gene expression level information is provided, and copy number information of genes can also be acquired, such that functions and regulatory mechanisms of genomes and the impact of copy number variations on gene expression can be better understood; and the method is of great significance for research on genetic diseases, rare diseases and complex diseases.

The invention discloses application of a reagent for detecting GPR174 gene expression level in CD4 + T cells and / or B cells in preparation of a product for detecting toxic diffuse goiter. The invention provides a detection marker for a toxic diffuse goiter (Graves disease). The detection marker is GPR174 expressed in a CD4 + T cell or a B cell. Experiments prove that compared with healthy people, the expression of GPR174 in CD4 + T cells or B cells in the body of a Graves disease patient is remarkably up-regulated, and the expression level of GPR174 in the CD4 + T cells and the B cells is in positive correlation with the levels of Graves disease thyroid functions FT3 (free triiodothyronine), FT4 (free tetraiodothyronine) and an autoantibody TSAb (thyroid stimulating hormone receptor stimulating antibody). The expression level of the GPR174 in the CD4 + T cell and the B cell is highly related to the occurrence of the Graves disease, and the expression level of the GPR174 in the CD4 + T cell and the B cell can be used as a detection indication of the Graves disease, so that a new thought is provided for detection and treatment of the Graves disease.

The invention relates to a method for rapidly detecting the cell adhesiveness of probiotics and application of the method. The method comprises the following steps: adding the probiotics into adherent culture cells, incubating, discarding liquid, digesting and collecting the cells by pancreatin, extracting total DNA (Deoxyribonucleic Acid), performing qPCR (Quantitative Polymerase Chain Reaction) amplification, and calculating the adhesion quantity of the probiotics on a single cell according to a Ct value. The invention provides a method for rapidly detecting the cell adhesion rate of probiotics by using a qPCR (quantitative polymerase chain reaction) technology, which comprises the following steps of: extracting total genome DNA (deoxyribonucleic acid) after the probiotics are adhered to cells on the basis of the gene expression difference of prokaryotic and eukaryotic cells, and detecting the specific gene expression level of the probiotics and the cells by using the qPCR technology. The cell adhesion ability of the probiotics is evaluated by calculating the ratio of the gene expression quantities of the two, and the method has the characteristics of high efficiency, rapidness, high accuracy and the like.

The invention provides a method for regulating and controlling the expression intensity of a prpB gene promoter, specifically, betaine is added into an expression system containing the prpB gene promoter, and the expression intensity of the prpB gene promoter can be regulated and controlled to be improved in the logarithmic phase and / or reduced in the stable phase of microbial growth. By utilizing the method, the expression level of a target gene in an expression system can be accurately regulated and controlled.

The invention relates to an application of bifidobacterium breve i1088 or a postbiotic thereof in improvement of cognition. The bifidobacterium breve i1088 or the afterbiogen of the bifidobacterium breve i1088 is used for preparing a product for enhancing the oxidative stress capacity of mammalian cells, improving the in-vitro free radical scavenging level of mammals and changing at least one of the expression levels of mammalian cells Nrf2 and anti-oxidation related genes. The invention aims to explore the potential of the bifidobacterium breve in the aspect of improving the Alzheimer's disease and provide a safer and more effective nutritional supplement scheme for middle-aged and elderly people.

The invention discloses a drug-resistant marker for treating prostatic cancer olaparib and application of the drug-resistant marker, and relates to the technical field of medicine, and the marker is a PCAR1 gene. Research finds that the PCAR1 gene is remarkably and highly expressed in prostate cancer Olaparil drug-resistant cells and clinical drug-resistant samples, and can be used as a key marker for judging the drug resistance of prostate cancer patients to Olaparil. By detecting the expression level of the PCAR1 gene, the treatment reaction of a patient to the olaparil can be effectively predicted, and a basis is provided for clinically and accurately selecting a treatment scheme. Meanwhile, the specific siRNA designed aiming at the PCAR1 gene can obviously inhibit the expression of the PCAR1 gene, and can effectively reduce the drug resistance of cancer cells to olaparione, promote the apoptosis of the cancer cells and inhibit the proliferation of the cancer cells when being combined with the olaparione for use. Based on the siRNA, the invention prepares a pharmaceutical composition containing the siRNA and olaparil, and provides a new drug combination scheme for the treatment of prostatic cancer.

A model construction method, a biological age prediction method and apparatus, and a storage medium, the model construction method comprising: generating a first data set, the first data set comprising gene expression level and methylation level data corresponding to a plurality of genes, selecting a plurality of candidate genes from the first data set, the gene expression level corresponding to each candidate gene is in significant negative correlation with the methylation level; a training data set and a verification data set are generated, the training data set comprises methylation level data of the multiple first genes, and the verification data set comprises methylation level data of the multiple second genes; taking an intersection of the candidate gene, the first gene and the second gene to obtain an intersection gene; and constructing a model by using methylation level data corresponding to a plurality of intersection genes in the training data set and the verification data set as input features. According to the method, the gene with biological significance is selected as the input feature of the model, so that a genetic and biological basis is added for the model, and the robustness of the model is enhanced.

The invention belongs to the technical field of lake and reservoir water source risk prediction, and provides a lake and reservoir water source algae bloom risk early warning system based on a 16S / 18SrRNA gene expression quantity threshold, and the method comprises the following steps: S1, collecting a sample from a lake and reservoir water source surface layer water body; s2, using 7-gate water bloom algae specific primers for blue-green algae, green algae, diatom, euglena, dinoflagellate, chrysophyta and cryptoalga; s3, based on the qPCR standard curve, calculating the copy number of the 16SrRNA / 18SrRNA gene of the seven water bloom algae; s4, when the gene expression quantity of a certain algal bloom algae continuously reaches 106-7 copy number / mL for 7-12 days, determining that the algal bloom algae is in a window phase; according to the threshold value of the gene expression quantity of the water bloom algae 16SrRNA / 18SrRNA, the window period of water bloom algae cells can be accurately recognized, so that early warning of the algae bloom risk in the lake and reservoir water source is achieved, and early warning of algae bloom outbreak in the lake and reservoir water source is achieved one week or above in advance.

The invention discloses an application of NFIB and a downstream target gene thereof in preparation of a hepatic fibrosis detection reagent and / or kit. The downstream target gene provided by the invention is a TEKT1 gene. Researches find that the expression of NFIB in hepatic fibrosis is reduced; after the NFIB is over-expressed, the expression level of the TEKT1 gene is obviously reduced, and the progress of hepatic fibrosis is further blocked. Therefore, the NFIB and the downstream target gene TEKT1 thereof are taken as detection targets and can be used for preparing a hepatic fibrosis detection reagent and / or kit, and hepatic fibrosis can be screened and the severity of fibrosis can be evaluated by detecting the expression levels of the NFIB and the TEKT1 in a sample to be detected.

The invention relates to armadillidiam vulgare derived carbon quantum dots as well as a preparation method and application thereof, the armadillidiam vulgare derived carbon quantum dots are characterized in that the particle size of the armadillidiam vulgare derived carbon quantum dots is 2.2-6.0 nm, and the armadillidiam vulgare derived carbon quantum dots can inhibit pain response, reduce oxidative stress level and reduce inflammatory factor gene expression level. The armadillidiam vulgare derived analgesic carbon quantum dots inhibit irritant movement and oxidative damage in a pain model, and reduce inflammatory factors and pain pathway gene expression; the oxidative stress level is reduced, and the effects of relieving pain, resisting inflammation and resisting oxidative stress are achieved.

The invention provides an ovarian cancer accurate diagnosis and personalized treatment method based on data integration, and the method comprises the steps: employing a feature extraction algorithm to generate a multi-dimensional feature vector set containing a gene expression level, an image texture parameter, a symptom score and a pathological classification according to a structured data set; performing dimension reduction processing on the multi-dimensional feature vector set through a feature fusion module to obtain a low-dimensional feature representation vector; performing grouping and risk prediction on the patient data by adopting a classification algorithm according to the low-dimensional feature representation vector, and generating a patient subgroup classification and disease risk layering result; according to the patient subgroup classification and the disease risk layering result, a scoring model is adopted to generate a diagnosis scoring result and a personalized treatment recommendation scheme, and a diagnosis report is output.

The invention provides a plant lactobacillus IOB602 for down-regulating an angiotensin receptor gene expression level and an application thereof, and relates to the technical field of microorganisms, the classification name of the plant lactobacillus IOB602 is plant lactobacillus, the plant lactobacillus IOB602 is preserved in China General Microbiological Culture Collection Center on June 29, 2018, called CGMCC for short, and the preservation number of the plant lactobacillus IOB602 is CGMCC. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.16021. By down-regulating the expression level of an angiotensin II receptor type 1 AT1R gene, a PI3K / AKT1 / eNOS / NO signal channel is activated, the activity of angiotensin converting enzyme is inhibited, vasodilation is enhanced, and blood pressure is reduced.

The invention belongs to the technical field of biological genetic engineering, and relates to a high-risk myelodysplastic syndrome screening gene and a diagnostic kit thereof. The invention provides an application of detecting YTHDF2, METTL3 and NAT10 gene expression of bone marrow cells of high-risk MDS patients as a specific molecular marker for screening the high-risk myelodysplastic syndrome, and by detecting mRNA expression levels of the YTHDF2, METTL3 and NAT10 genes, the screening of the patients in the high-risk myelodysplastic syndrome is facilitated. Meanwhile, treatment and prognosis evaluation of patients with high-risk myelodysplastic syndromes can be better guided. Only the bone marrow fluid of the patient needs to be extracted and collected during bone marrow cytology examination of the patient, the patient compliance is good, the bone marrow fluid is selected from human bone marrow fluid mononuclear cells, the detection result is more accurate, and the method is suitable for clinical large-scale application.

The invention provides application of a CD47 gene in detection of pig litter size phenotype and application of an SNP site of the CD47 gene in gene expression regulation. It is found that the CD47 gene is regulated and controlled by the SNP site rs323354626, it is further found that the rs323354626 site and the phenotype of the total litter size of sows show the highest significance, and it is indicated that the CD47 gene and the total litter size of the sows have large correlation. In three main commercial varieties of pigs, namely Duroc, Landrace and Yorkshire, the CD47 expression level in testis tissues is negatively correlated with the litter size, which indicates that the expression mode of the CD47 gene may influence the reproductive performance of the pigs, and the litter size phenotype of the pigs can be detected by detecting the CD47 gene expression level in the testis of the breeding pigs or somatic cells of the sows. Or the litter size of the pigs is influenced by regulating and controlling the expression level of the CD47 gene through the rs323354626 site, and the discovery lays a foundation for improving the breeding efficiency of the pigs, especially for a breeding method for increasing the litter size.

The present invention relates to methods of predicting the response of prostate cancer subjects to neohelper androgen deprivation therapy. The method is based on prediction results based on gene expression levels identified herein. The present invention provides a method for determining whether neoadjuvant androgen deprivation therapy is useful for a subject and thus should be administered. Further provided is the use of a kit for determining the expression level for predicting the response of a prostate cancer subject to neohelper androgen deprivation therapy.

The invention discloses an application of RsNRAMP5 heavy metal transporter protein in cadmium absorption and active oxygen accumulation of radish, which comprises the following steps: (1) cloning RsNRAMP5 by using radish cDNA as a template, and the cDNA sequence of the RsNRAMP5 is as shown in SEQ ID NO.1; (2) the increase of Cd treatment time (6h, 12h, 24h) on the expression level of the RsNRAMP5 gene is obviously up-regulated; (3) overexpression of RsNRAMP5 significantly increases the absorption of Cd < 2 + > in yeast cells; (3) the activity of the RsNRAMP5 promoter in the radish cotyledons can be obviously induced through treatment of 100 [mu] M Cd; and (4) instantaneously transforming the radish cotyledons by using an agrobacterium injection method, analyzing the change of the content of H2O2, O2 <.-> and Cd in the radish cotyledons under the treatment of CdCl2 and the inhibition expression of RNAi, and discovering that the overexpressed RsNRAMP5 promotes the absorption of Cd and the accumulation of active oxygen in the radish cotyledons. The invention provides a theoretical basis for breeding low Cd accumulation radish germplasm by using a genetic engineering technology in future, and has a very high application value.

The invention discloses a method for evaluating the detection effect of a fusion gene detection method, and belongs to the technical field of evaluation based on the detection effect. According to the invention, a detection reference substance I and a detection reference substance II are constructed, and the detection reference substances are connected with a reference gene segment in equal molecular number proportion, so that the detection condition under the condition of equivalent reference gene expression level can be realized, and whether the sample quality reaches the detection standard or not can be deduced; the measurement of the lowest detection quantity of the fusion gene relative to the expression quantity of the reference gene is realized; the first detection reference is used for detecting sensitivity analysis of the fusion type by the detection method of the fusion gene to be detected, and the second detection reference is used for verifying whether a detection result of the detection method of the fusion gene to be detected is a false negative result or not. And the method has a very good application prospect.

The invention relates to application of CXCL4 derived from serum exosome in colorectal cancer diagnosis, treatment selection and prognosis evaluation, and belongs to the technical field of tumor treatment. According to the invention, the expression level of the CXCL4 gene derived from the serum exosome for immunotherapy of patients suffering from colorectal cancer is obviously higher than that of the CXCL4 gene derived from the serum exosome for immunotherapy of the patients suffering from colorectal cancer, and the CXCL4 gene can be used as a marker for distinguishing the non-sensitive type and the sensitive type for immunotherapy of the patients suffering from colorectal cancer and is used for guiding a subsequent treatment scheme.

The invention relates to the technical field of biological medicine, in particular to an ulcerative colitis disease clearance evaluation molecular marker and application thereof. By detecting the expression level of the HMGCS2 gene in a sample, the ulcerative colitis disease clearance state and disease activity period can be evaluated, and the method is used for curative effect monitoring, recurrence prediction and individualized treatment strategy formulation of ulcerative colitis and has important clinical application value and market prospect.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention discloses a spatial transcriptome data deconvolution method, which comprises the following steps of: (1) filtering spatial transcriptome data and single cell data, and reserving a part containing common gene expression information; (2) counting gene expression of each cell type based on the filtered single cell data and corresponding single cell annotation data, calculating the average gene expression level of each cell type, and constructing a reference matrix; (3) constructing an optimization model by taking space communication and space cell composition as decision variables; and (4) solving the optimization model by using an optimization algorithm with the aim of minimizing the difference between the predicted gene expression value and the real gene expression value to obtain joint inference of space deconvolution and a space communication mode. According to the method and the device, the deconvolution task of the space transcriptome data can be finished with high precision, and meanwhile, accurate inference of space communication is realized.

The invention discloses siRNA (small interfering Ribonucleic Acid) of a targeted urate transporter 1 and application of the siRNA. According to the invention, siRNA drug design is carried out on the URAT1 gene to obtain siRNA composed of positive-sense strand RNA and antisense strand RNA, siRNA capable of effectively silencing the gene expression level of URAT1 is screened from the siRNA, and part of siRNA sequences even silence the gene expression level of URAT1 to about 20%. The invention further discloses the modified siRNA which is obtained by chemically modifying the siRNA sequence and can effectively silence the gene expression level of URAT1, and a recombinant vector containing the siRNA or the modified siRNA. The siRNA, the modified siRNA and the recombinant vector containing the siRNA or the modified siRNA provided by the invention have the application in the aspects of preparation of gene therapy medicines for treating hyperuricemia or gout and the like.

The invention provides a VvYUC10 gene, the nucleotide sequence of the VvYUC10 gene is as shown in SEQ ID No.1, or the nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function; the invention also provides an application thereof. The grape VvYUC10 gene has the advantages that the function of the grape VvYUC10 gene is verified, it is found that the content of auxin in calluses of overexpressed VvYUC10 arabidopsis thaliana and grapes under salt stress is increased, the oxidation resistance is enhanced, and it is further found that the expression quantities of the VvRD22 gene, the VvDREB2A gene, the VvRD29A gene, the VvP5CS gene, the VvCAT1 gene and the VvSOD gene are increased; finally, it shows that the VvYUC10 gene positively regulates and controls the auxin signal channel to positively resist external adverse environmental factor salinization, and a theoretical reference basis is provided for grape salt-tolerant gene mining and breeding.