89 results about "Ecdysone" patented technology

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, and its N-oxides, and suitable salts thereof

and a component (b) wherein the component (b) is at least one compound or agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and suitable salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

In accordance with the present invention, there are provided various methods for modulating the expression of an exogenous gene in an isolated cell and in a mammalian subject employing modified ecdysone receptors. Also provided are modified ecdysone receptors, as well as homomeric and heterodimeric receptors containing same, nucleic acids encoding invention modified ecdysone receptors, modified hormone response elements, gene transfer vectors, recombinant cells, and transgenic animals containing nucleic acids encoding invention modified ecdysone receptor.

In accordance with the present invention, there are provided various methods for modulating the expression of an exogenous gene in a mammalian subject employing modified ecdysone receptors. Also provided are modified ecdysone receptors, as well as homomeric and heterodimeric receptors containing sane, nucleic acids encoding invention modified ecdysone receptors, modified ecdysone response elements, gene transfer vectors, recombinant cells, and transgenic animals containing nucleic acids encoding invention modified ecdysone receptor.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, an N-oxide, or a salt thereof, Formula (1) and (b) at least one invertebrate pest control agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

The invention relates to a method for extracting a composition containing nano-oligopeptide from cordyceps militaris as well as the composition extracted by the method. In the method, the cordyceps militaris is hydrolyzed by a complex enzymatic method to obtain the small molecular nano-oligopeptide. The invention is characterized in that the molecular weight of the nano-oligopeptide is mainly distributed within 100-1000D; and preferably the Gaussian distribution peak area percentage of the nano-oligopeptide the molecular weight of which is 100-500 D in the composition is more than 50%. The cordyceps militaris oligopeptide extract provided by the invention reserves other active ingredients such as cordycepin, ecdysone, adenosine, cordyceps polysaccharide, cordycepic acid, trace elements, vitamins and free amino acid. The invention further relates to an application of the composition containing the nano-oligopeptide extracted from the cordyceps militaris in preparing medicines, healthcare products and cosmetics.

The invention relates to a juvenile horse crab feed formula and a processing method thereof. The feed formula includes fish meal, soybean meal, cottonseed meal, rapeseed meal, wheat middling, cassava alpha starch, squid extract, shrimp shell meal, fish oil, phospholipid, feed attractant, ecdysone, antioxidant, mildew preventive, premix, monocalcium phosphate and neutral phytase. The method for preparing the compound feed is as follows: the materials are weighed according to formula proportion, ground, uniformly mixed in a mixer and produced into pellets, the diameter of the pellet is 3mm, thelength of the pellet is larger than or equal to 3cm, and the pellets are dehydrated and cooled by cold wind. The method for adding the neutral phytase depends on the processing temperature, and the neutral phytase can be directly put into the materials before pelletization, or can be sprayed on the pellet feed after pelletization. The nutrition of the feed is rich, and palatability is good; the addition of the phytase increases the utilization rate of phytate phosphorus, the dosage of the monocalcium phosphate is reduced, consequently, the cost is reduced, the discharge of phosphorus in wateris reduced, environment pollution is reduced, and ecological equilibrium is protected.

The invention relates to water splashing compound preparation for boosting exuviation of shrimps and crabs, hardening shells, preventing diseases and resisting stresses and belongs to the technical field of aquiculture. Glucose, sodium chloride, monopotassium phosphate, magnesium sulfate, calcium chloride, calcium lactate, taurine, trimethyl glycine, vitamin C, ecdysone and Chinese herbal medicament are chosen as the compound components of the water splashing compound preparation. The compound components are weighted in proportion, and then are mixed uniformly and packaged in batch, and finally are packaged and placed in a warehouse. Various nutrient components which are safe and nontoxic to shrimps and crabs are adopted by the invention; the functions of boosting exuviation, supplementing calcium and hardening shells, preventing diseases and eliminating stresses, and the like, are integrated; the components are clear; the effect is stable; the water splashing compound preparation can be directly splashed and conveniently used; the bioavailability is high and is not influenced by environmental condition; and after the water splashing compound preparation is used, the normal physiological functions and immunologic functions of cultivated shrimps and crabs are quickly recovered, thereby achieving the functions of boosting exuviation, supplementing calcium and hardening shells, preventing diseases and eliminating stresses, and increasing the survival rate and growth rate of the cultivated shrimps and crabs.

The invention discloses a method for extracting ecdysone from common cyanotis roots and the ecdysone thereof, belonging to the technical field of the extraction and the separation of effective components of plants. The method comprises the following steps of: decocting 0.5-1kg of common cyanotis roots by 10L of water, or extracting by using 1-95 percent methanol or ethanol, and the ratio of the using amount of the methanol or the ethanol to the common cyanotis roots is 5L-10L to 1kg; filtering, decompressing and concentrating to obtain liquid with the relative density of 1.05-1.20; regulating a pH value to 6-10 by using alkali; then extracting by a mixed solvent with normal butanol and ethyl acetate, wherein the ratio of the normal butanol and the ethyl acetate is 4:6-0.5:9.5; washing, decompressing and concentrating to obtain a fluid extract with the relative density of 1.2-1.3; adding 50-95 percent methanol or ethanol for dissolving and dilution, passig through overhigh alkali type anion exchange resin, and eluting, concentrating and drying in a decompressing way to obtain the ecdysone. Compared with a conventional method, the method enhances the yield by 1.16-1.38 percent.

The invention discloses a domestic silkworm outdoor cocooning method on cocoon bed, which comprises the steps: a. setting a simple cocoon bed climbing shed and cocoon bed rack in shed, b. adding mulberry leaf sprayed with ecdysone, levomycetin water solution when 2-5% maturing silkworm occur, c.next day morning, collecting maturing cocoon in hole of grid cocoon bed, d. transferring grid cocoon bed full of maturing silkworm to cocoon bed rack of outdoor simple cocoon bed climbing shed, keeping grid sheet hanging in south-north direction e. maturing silkworm entering hole and spin and cocooning outside, transferring to inside. The special grid cocoon bed includes horizontal grid sheet and longitudinal grid sheet, the said horizontal grid sheet and longitudinal grid sheet interlock forming grid rack. Two horizontal grid sheets outside rack are connected with hanging rung longer than horizontal grid sheet. The invention possesses the advantages of low investment, good cocoon quality, low intensity of labor, high production efficiency.

The invention relates to feed, particular to feed for swimming crabs. The feed is prepared from raw materials as follows: shrimp meal, fish meal, bone meal, silkworm chrysalis meal, marine animal protein hydrolysate, cuttlefish paste, shrimp shell meal, seaweed meal, soybean meal, peanut meal, corn flour, wheat flour, sugar, squid viscera meal, fish oil, soybean oil, soybean phospholipid meal, choline chloride, decavitamin, compound minerals, monocalcium phosphate, an ecdysone premix, immunopolysaccharide, an antibacterial peptide, bacillus, lactobacillus, non-starch polysaccharide enzyme, strepogenin, a phagostimulant, an antioxidant and a mildew preventive. The feed has balanced nutrition, is antibacterial and meets feeding habits of the swimming crabs, mutual killing of the swimming crabs due to insufficient feed, molting period or overlarge group is avoided, the swimming crabs cannot cross over ponds to escape, the crab incidence rate is low, the survival rate is high, the growth speed is high, and the individual weight of the swimming crabs is higher than that of wild swimming crabs.

The invention discloses formulated hatch feed of young penaeus, which consists of the following components by weight percent: 30 percent to 65 percent of excellent white fish meal, 7 percent to 5 percent of fish oil, 10 percent to 20 percent of steam fish meal, 3 percent to 10 percent of cuttlefish visceral meal, 2 percent to 10 percent of soybean meal, 0.5 percent to 3 percent of vitamin mixture, 2 percent to 10 percent of peanut meal, 0.5 percent to 3 percent of mineral substance mixture, 10 percent to 30 percent of flour, 0.2 percent to 2 percent of astaxanthin, 0.2 percent to 2 percent of phospholipid and 0.1 percent to 2 percent of ecdysone. Aiming at the growing and physiological digestion characteristics of the young penaeus larvae during hatching period, the formulated hatch feed selects feed materials with high utilization rate, is prepared through ultramicro-pulverization technology, promotes rapid growth of the young penaeus effectively, improves survival rate of transformation of the young penaeus, and strengthens the resistance ability of the young penaeus. The formulated hatch feed has reliable material source, stable quality and low price.

The invention discloses a method for extracting ecdysone from spinacia oleracea. The method comprises the following steps: taking a to-be-detected sample; grinding liquid nitrogen into powder and adding a mixed solution of MeOH-CHCl3 in a volume ratio of 9:150ml or 100% MeOH solution until the solution is 50ml; vibrating at 37 DEG C for 2 hours and filtering by using silica gel powder; adding less NaCl and 50ml of n-Hexane to extract for two times; collecting an MeOH layer and drying by using a rotary evaporator; dissolving the sample again by using isopropanol; filtering and carrying out high performance liquid chromatography; determining the content. According to the method, an extract is subjected to the high performance liquid chromatography; the extraction speed is rapid and the extraction efficiency is high; the trace extraction of the extract can be realized.

The invention discloses a preparation method and an application of an ecdysone-rich plant standardized extract. The preparation method comprises the steps of: extracting ecdysone-rich plant materials using ethanol under reflux, filtering, concentrating and centrifuing; decolorizing the centrifuged supernatant, purifying by macroporous resin column chromatography, concentrating and drying to obtain the standardized extract. The method is simple and feasible, short in production period, and stable and controllable in quality, and is easy for preparation and industrial production in a standardized and batch manner; and the similarity of the extract obtained by the method is more than 99% by HPLC (high performance liquid chromatography) fingerprint spectrum detection. The extract can be applied in preparing fixed preparations serving as drugs for reducing blood sugar and resisting rheumatoid arthritis, can be used as a raw material for natural cosmetics and can be applied in animal breeding industry.

The present invention provides a screening method of an ecdysone analogue. The method comprises the steps of: 1) determining an active pocket; S2) screening SPECS library according to a receptor-ligand pharmacophore model and a ligand pharmacophore model, and establishing a database 1; S3) performing clustering and drug-likeness analysis of compounds in the database 1, and establishing a database2; S4) performing docking of compounds in the database 2 and the receptor to establish a database 3, screening the receptor binding activity of the compounds in the database 3, and obtaining virtual screening compounds; S5) screening binding activity of the compounds and the receptor in the database 2, and obtaining virtual screening compounds; S6) performing determination of ecdyson receptor binding activity of the virtual screening compounds, and obtaining a hit lead compound; and S7) performing similarity retrieval of the hit lead compound. The virtual screening method can reduce the numberof the experiment screening compounds, can shorten the research and development period and can reduce the economic cost.

The invention relates to ecological feed for factory-cultivated Penaeus vannamei. The feed comprises, by weight, 60 to 70% of a protein source raw material, 6 to 9% of a fat source raw material, 20 to25% of a carbohydrate raw material, 1.0 to 3.0% of vitamins, 1.0 to 3.0% of a mineral salt, 0.1 to 0.2% of an antioxidant, 0.1 to 0.2% of ecdysone and 0.05 to 0.1% of an anti-stress substance. The ecological feed for factory-cultivated Penaeus vannamei ensures the normal growth of Penaeus vannamei, significantly increases the survival rate and weight gain rate of Penaeus vannamei, improves the anti-stress ability and disease resistance of Penaeus vannamei, solves the serious problems of over high nitrogen and phosphorus content and environmental pollution in Penaeus vannamei cultivation, significantly reduces nitrogen and phosphorus discharge of aquaculture water and has good ecological benefits.

The invention provides a method for making cultivated silkworm climb the fascicle itself which comprises, when ripe silkworm accounts to 5-10% of a batch of silkworms for uniform astogeny, mounting ripe silkworms separately under temperature of 24+-2 deg. C, feeding ecdysone to the large amount of residual silkworms at 20-21 clock, after 10-12 hours, ripening the whole batch of silkworms collectively, spreading a layer of cordate houttuynia in a silkworm stand, placing the mounting unit on the silkworm stand, depositing the mounting unit after 20 minutes, and collectively mounting the small number of ripe silkworms.

The invention discloses a method for establishing an ecdysone-enriched ajuga multiflora bunge suspension cell line. The method comprises the following steps: 1, placing an ajuga multiflora bunge explant on an induction culture medium for induction culture to obtain a first callus; 2, placing the first callus on a subculture medium for carrying out subculture for more than 5 times to obtain a second callus; 3, placing the second callus on a suspension culture medium for suspension shake culture; and 4, harvesting cells and extracting ecdysone. By the method disclosed by the invention, the ajuga multiflora bunge suspension cell line is firstly established and haploid plants having a full set of parental genes can be quickly and efficiently obtained. The method is conductive to deeply analyzing the mechanism for synthesizing ecdysone from ajuga multiflora bunge from the perspective of cytology and lays the technical foundation for the genetic transformation and genetic modification of ajuga multiflora bunge.

The invention relates to feed, in particular to feed for mixed breeding of crabs and shrimps. The feed is prepared from the following raw materials in parts by weight: 11-42 parts of snail meat, 9-28 parts of soya bean meal, 20-30 parts of lean pork, 25-35 parts of corn flour, 5-10 parts of edible oil, 8-29 parts of rice bran, 5-21 parts of peanut meal, 6-18 parts of fish meal, 5-9 parts of silkworm chrysalis, 3-9 parts of medical stone, 5-15 parts of wheat bran, 0.3-0.6 part of radix bupleuri, 0.2-0.5 part of astragalus, 1-2 parts of table salt, 5-7 parts of sugar, 0.05-0.1 part of ecdysone and 0.03-0.05 part of a mildew preventive. The feed disclosed by the invention is comprehensive and balanced in nutrition and achieves the effects of solving the problem of mismatching of the feed for mixed breeding of crabs and shrimps, improving the utilization ratio of the feed and reducing aquaculture water pollution caused by excessive feed; moreover, the feed disclosed by the invention has the effects of killing insects, inhibiting bacteria and purifying water, the survival conditions of the crabs and shrimps are improved, and the yield, quality and breeding benefits are improved.

The invention provides an azadirachta indica cell suspension culture medium and a cell suspension culture method. According to the azadirachta indica cell suspension culture medium, based on a basic culture medium, component matching is optimized, and a certain amount of sodium vanadate, L-cysteine, tyrosine, nicotinamide, dithiothreitol, beta-D-galactose, cyclohexanol phosphate, diethylaminoethylhexanoate, 6-BA, ecdysone, sodium nitroprusside, docusate sodium, flaccid knotweed herb juice and henry munronia herb juice is added; the cell suspension culture method comprises the following stepsof 1) obtaining of sterile test-tube plantlets; 2) induction and subculture for calluses; 3) cell suspension culture. By adopting the culture medium and the culture method, not only can the stable azadirachta indica cell suspension culture system be obtained, but also the biomass of suspension cells and the content of azadirachtin in the suspension cells can be significantly improved, the technical support is provided for azadirachtin production through large-scale azadirachta indica cell culture, and important application value is achieved.

The invention discloses a monoclonal antibody capable of being specifically bound with apolygus lucorum AlEcR-A protein. The invention also discloses a mouse hybridoma cell line with a preservation number of CCTCC NO: C2016206. The monoclonal antibody provided by the invention can be specifically bound with apolygus lucorum total protein and AlEcR-A recombinant protein. The invention also discloses an application of the monoclonal antibody in comprehensive treatment of apolygus lucorum. The invention also discloses a detection kit comprising the abovementioned monoclonal antibody.

The invention belongs to the technical field of phytochemistry. The invention discloses a method for simultaneously separating schaftoside, vichnine-2 and ecdysone and application. Elsholtzia rugulosais used as an extraction raw material, 90% ethanol is used as an extraction solvent, the extracting solution is concentrated under reduced pressure, and the extracting solution is extracted with petroleum ether, ethyl acetate and n-butyl alcohol in sequence; the concentrated n-butyl alcohol extraction part is coarsely separated by using AB-8 macroporous resin to obtain a 50% ethanol elution part;and ODS column chromatography separation is performed on the eluted part to obtain an ecdysone monomer, impurity-containing schaftoside and impurity-containing vichnine-2, and silica gel column chromatography separation and purification are respectively performed on the impurity-containing schaftoside and impurity-containing vichnine-2 to respectively obtain a schaftoside monomer and a vichnine-2monomer. The method is short in separation and purification process, simple to operate and easy to implement, and plant resources and extraction and separation cost are greatly saved; and great economic benefits can be generated, and the development of industries in related fields can be promoted.

The invention discloses a unilaterally-substituted phenyl urea compound (as shown in formula I) and an application thereof. The structural formula of the compound is as shown in the formula I in description, wherein R1, R2 and R3 in the formula I represent hydrogen, C1-C4 alkyl, C1-C4 halo-alkyl, C1-C4 alkoxy, C1-C4 alkyl sulfenyl, halogen, nitryl, cyan and hydroxyl. The compound VS01 disclosed by the invention has an obvious ecdysone antagonistic activity on 20E and also has a certain killing activity on lepidopteron; the compound is simple in structure and large in modification space, and can be used for in-depth study as an ecdysone antagonist type lead compound with a totally new structure.

The invention discloses a method for extracting ecdysone from decumbent bugle herb. The method comprises the following steps of: (1) refluxing and leaching the decumbent bugle herb for 2 to 3 times by using water in an amount which is 6 to 10 times that of the decumbent bugle herb, leaching for 1 to 3 hours every time, filtering, mixing filtrate and concentrating under reduced pressure; (2) centrifuging, and reserving supernate; (3) adding ethanol precipitate impurities into the supernate; (4) centrifuging, reserving the supernate, and concentrating under reduced pressure; (5) loading on CAD-40 macroporous resin; (6) eluting by using 5 percent ethanol and 35 percent ethanol sequentially, and reserving a desorption liquid of 35 percent ethanol; (7) concentrating under reduced pressure until the desorption liquid does not have alcohol taste, extracting for 2 times by using equal amount of ethyl acetate, and reserving an ethyl acetate layer; (8) evaporating and crystallizing under reduced pressure; and (9) drying crystals to obtain the ecdysone of the finished product. The invention provides the method for extracting the ecdysone from the mature decumbent bugle herb, and due to the adoption of water leaching, the using amount of the ethanol is small, and the cost is saved; and the impurities are removed in modes of ethanol precipitation and the elution of low-concentration ethanol to ensure the purity of the ecdysone of the finished product.