9 results about "Ecdysone" patented technology

The invention relates to a pesticide composition containing ecdysone compounds and application of the pesticide composition. The pesticide composition is composed of flufeniprole and methoxyfenozide or tebufenozide. The pesticide composition has an obvious synergistic effect, and the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, tryporyza incertulas, rice leaf folders, plant hoppers, prodenia litura, beet armyworms, plutella xylostella, cabbage caterpillars, aphids, aleyrodids, thrips and flea beetles.

The application discloses an information pheromone binding protein derivative peptide and a biosensor capable of effectively monitoring the sex pheromone of Hyphantria cunea, and belongs to the field of biosensors. At present, the monitoring of H. cunea is usually achieved by means of sample plot investigation, sex lures and other means, and problems such as poor timeliness, high labor cost and insufficient data continuity exist. In order to solve the above problems, the information pheromone binding protein derivative peptide provided by the application has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, single-walled carbon nanotubes are attached to the electrode, and the information pheromone binding protein derivative peptide is further connected. The sensor can specifically detect the sex pheromone of H. cunea and has no response to 17 kinds of plant volatiles, and can detect the sex pheromone released by as low as five live female H. cunea under laboratory conditions. The sensor has application potential in the fields of early-stage pest monitoring, improvement of prevention and control efficiency and guarantee of ecological safety.

The invention discloses an ecdysone nanoemulsion for improving pupation uniformity of protaetia brevitarsis as well as a preparation method and application of the ecdysone nanoemulsion, and relates to the technical field of insect breeding. The ecdysone nanoemulsion is prepared from the following raw materials: ecdysone, ultrapure water, ethanol, sodium bicarbonate, a surfactant, a cosurfactant and edible oil. According to the ecdysone nanoemulsion, 5% of 3-instar larva groups with the average weight of 2.5 g or above, or 80-day larva groups after incubation, or 3-instar larva groups in the same batch are in an aged state, the ecdysone nanoemulsion is continuously used for 7 days by being mixed with feed, meanwhile, the effective content range of ecdysone is within the mixing concentration range of 20-40 mg / kg, the pupation uniformity of the larva groups can be helped to be improved, and the pupation uniformity of the larva groups can be improved. The method is beneficial for improving the population uniformity of the protaetia brevitarsis and expanding the population of the protaetia brevitarsis within a short time, provides technical guarantee for treating agricultural and forestry wastes by applying the protaetia brevitarsis larvae, and has a good application prospect.

This invention discloses a novel plant-derived ecdysone antagonist derived from Dryopteris crassirhizoma, its preparation method, and its application, belonging to the field of biological control of agricultural pests and green pesticide technology. The novel plant-derived ecdysone antagonist disclosed in this invention has the molecular formula C0.05. 21 H 24 O8. This invention discloses a novel plant-derived ecdysone antagonist derived from Dryopteris crassirhizoma, targeting the insect's unique developmental system. It exhibits high safety for non-target organisms such as mammals and pollinating insects, and is readily degradable in the environment, perfectly meeting the dual requirements of agricultural product safety and ecological protection. This invention not only provides a novel pesticide but also establishes a complete technical system from target discovery and efficient screening to green application.

The invention discloses miR-285-3p for preventing and treating spodoptera frugiperda and application of the miR-285-3p. MiRNA, miR-285-3p targeting a key gene for growth and development of the spodoptera frugiperda is screened for the first time, and after an analogue agomiR-285-3p of the miRNA, miR-285-3p is injected, the agomiR-285-3p is found to be capable of inhibiting normal expression of ecdysone induced protein 74EF (Ecdyson-induced protein 74EF, Eip74EF) gene of the spodoptera frugiperda, and the agomiR-285-3p can be used for inhibiting the growth and development of the spodoptera frugiperda. Therefore, the growth and development of the spodoptera frugiperda larvae are slowed down, the feed intake is reduced, and the death rate is increased. In addition, by adding an analogue agomiR-285-3p into the feed, the growth of the spodoptera frugiperda can be inhibited, the food intake is reduced, and the death rate is increased. The invention provides a new target and technical support for establishing a new strategy for preventing and controlling the spodoptera frugiperda based on the miRNA.

The invention discloses a production process for crude extraction of 95% ecdysone from cyanotis arachnoidea, and particularly relates to the technical field of plant separation and extraction. The preparation process comprises the steps of raw material preparation, macroporous resin adsorption and crystallization purification. According to the method disclosed by the invention, high-purity extraction of the ecdysone and remarkable shortening of the production period are realized by adopting the steps of crushing and extracting the fresh cyanotis arachnoidea, adjusting the pH value by lime milk, adsorbing and desorbing by macroporous resin and the like. Compared with a traditional production process, the method has the advantages that the production cost is lower, the production efficiency is higher, and reliable technical support is provided for large-scale production of ecdysone. The invention also relates to an application of 55%-60% HPLC of the cyanotis arachnoidea extract. Through a solvent crystallization method, high-purity ecdysone can be further extracted from the Cyanotis arachnoidea extract. The method has the characteristics of high efficiency and practicability, and can provide a more stable raw material source for production of ecdysone.

The invention discloses a pheromone binding protein derived peptide for effectively detecting sex pheromones of fall webworms and a biosensor, and belongs to the field of biosensors. At present, methods such as sample plot survey and sex traps commonly used in fall webworm monitoring have the problems of poor timeliness, high labor cost, insufficient data continuity and the like. In order to solve the problem, the pheromone binding protein-derived peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, a single-walled carbon nanotube is attached to the electrode, and the electrode is also connected with the pheromone binding protein-derived peptide. The sensor can specifically detect sex pheromones of fall webworms, and has no response to 17 plant volatile matters. The method has application potential in the fields of early pest monitoring, prevention and control efficiency improvement, ecological safety guarantee and the like.

The invention discloses miR-2c-5p for preventing and treating spodoptera frugiperda and application of miR-2c-5p. MiRNA and miR-2c-5p targeting a key gene for growth and development of the spodoptera frugiperda are screened for the first time, and after an analogue agomiR-2c-5p of the miRNA and miR-2c-5p is injected, the agomiR-2c-5p is found to be capable of inhibiting normal expression of ecdysone-induced protein 78C (Ecdyson-induced protein 78C, Eip78C) gene of the spodoptera frugiperda, and the agomiR-2c-5p can be used for preventing and treating the spodoptera frugiperda. Therefore, the growth and development of the spodoptera frugiperda larvae are slowed down, the feed intake is reduced, and the death rate is increased. In addition, by adding an analogue agomiR-2c-5p into the feed, the growth of the spodoptera frugiperda can be inhibited, the food intake is reduced, and the death rate is increased. The invention provides a new target and technical support for establishing a new strategy for preventing and controlling the spodoptera frugiperda based on the miRNA.